what is the growing mechanism of filamentous fungi, such as n. crassa? project by thomas lew mentor:...
Post on 19-Dec-2015
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What is the growing mechanism of filamentous fungi, such as N. crassa?
Project by Thomas Lew
Mentor: Dr. Michael Freitag
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Why this important?
Advances knowledge of fungal biology
Could lead to a way to stop growth of infectious fungi (i.e. selective targeting)
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Examples of Infectious Fungal Diseases
Aspergillosis
Fungal pneumonia
http://www4.umdnj.edu/cswaweb/rad_teach/allasper.html
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Why N. Crassa?
One of the oldest genetic model
Filamentous
Easier to manipulate
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QuickTime™ and aYUV420 codec decompressor
are needed to see this picture.
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What we think happens:
Microtubule Organization Center (at nuclei)
Apical Growth Body
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Methods
• PCR – subcloning and N. crassa transformation
Replace Mal3 MT end binding protein with GFP-tagged Mal3 MT
Replace Tip1 MT binding protein with GFP-tagged Tip1 MT
• Microscopy
Track growth and direction
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Polymerase Chain Reaction (PCR)
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GFP
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http://www.nih.gov/science/models/neurospora/image.html
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Results
![Page 14: What is the growing mechanism of filamentous fungi, such as N. crassa? Project by Thomas Lew Mentor: Dr. Michael Freitag](https://reader030.vdocuments.mx/reader030/viewer/2022032704/56649d3e5503460f94a177a7/html5/thumbnails/14.jpg)
QuickTime™ and aH.264 decompressor
are needed to see this picture.
QuickTime™ and aYUV420 codec decompressor
are needed to see this picture.
![Page 15: What is the growing mechanism of filamentous fungi, such as N. crassa? Project by Thomas Lew Mentor: Dr. Michael Freitag](https://reader030.vdocuments.mx/reader030/viewer/2022032704/56649d3e5503460f94a177a7/html5/thumbnails/15.jpg)
Acknowledgements
Howard Hughes Medical Institute
Dr. Kevin Ahern
Dr. Michael Freitag
Dr. Michael Gross
Grant Farr
All the wonderful people at OSU that helped us out