what is immunofluorescence? - union college is immunofluorescence? •technique allowing the...

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What is Immunofluorescence? Technique allowing the visualization of a specific protein or antigen in cells or tissue sections by binding a specific antibody chemically conjugated with a fluorescent dye Immunofluorescence staining can be performed on cells fixed on slides and tissue sections. Immunofluorescence stained samples are examined under a fluorescence microscope or confocal microscope. Source: http://www.protocol-online.org/prot/Immunology/Immunofluorescence/

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Page 1: What is Immunofluorescence? - Union College is Immunofluorescence? •Technique allowing the visualization of a specific protein or antigen in cells or tissue ... IF.ppt Author: Jennifer

What is Immunofluorescence?

• Technique allowing the visualization of aspecific protein or antigen in cells or tissuesections by binding a specific antibodychemically conjugated with a fluorescent dye

• Immunofluorescence staining can beperformed on cells fixed on slides and tissuesections.

• Immunofluorescence stained samples areexamined under a fluorescence microscopeor confocal microscope.

Source: http://www.protocol-online.org/prot/Immunology/Immunofluorescence/

Page 2: What is Immunofluorescence? - Union College is Immunofluorescence? •Technique allowing the visualization of a specific protein or antigen in cells or tissue ... IF.ppt Author: Jennifer

Sample Image

Immunofluorescence of Anti-nuclear antibody

Source: http://clinicalchemistry.stanford.edu/allergy_autoimmune.html