What invention helped with many discoveries in science?

Agenda for Friday Sept 18th 1.Microscope notes2.Microscope lab

What’s my power?To calculate the power of magnification, multiply the power of the ocular lens by the power of the objective.

Eyepiece is 10x and objective is 10x what is total magnificaion?

Basic Rules and Tips

• Carry using arm and base• Flat Surface• Adjust light using diaphragm • Use stage clips• Focus with course adjustment on low and

medium power only!– High Power use fine adjustment only

How to make a wet-mount slide …

1 – Get a clean slide and coverslip from your teacher.

2 – Place ONE drop of water in the middle of the slide. Don’t use too much or the water will run off the edge and make a mess!

3 – Place the edge of the cover slip on one side of the water drop.

4 - Slowly lower the cover slip on top of the drop.

Cover Slip

Lower slowly

Comparing Powers of Magnification

We can see better details with higher the powers of magnification, but we cannot see as much of the image.

Which of these images would be viewed at a higher power

of magnification?

How do you calculate total magnification?

Agenda for Monday Sept 21st 1.Measure with microscope notes2.Labs

Worm lab due tomorrowQuiz TuesdayTest Thursday

Calculate Field of View

• Clear Ruler• Determine how many millimeters across• Convert to micrometers• 1mm=1000μm• So: if 1.5 mm across then field of view is1.5mm x 1000 μm = 1500 μm

1mm

one millimeter is the distance from the middle of one mark to the middle of the next mark.

Calculate Size of Specimen

• Figure out how many will fit across the field of view (estimate)

• Divide field of view length by how many fit across

Example:*About 8 fit across

– field of view is 800 μm – 800 μm/8 = 100 μm

Remember!

• Carry using arm and base• Flat Surface• Adjust light using diaphragm • Use stage clips• ONLY USE FINE ADJUSTMENT ON HIGH POWER!

How many microns(micrometers) is 3.2 mm?

Agenda for Tuesday Sept 22nd 1.Quiz2.What is life notes3.Finish labs

TEST Thursday

• Low power = 4 mm or 4000 μm• Medium power = 1.5 mm or 1500 μm• High power = .5 mm or 500 μm

Lab Questions

1. a. If I measure the low power field of view to be 7 mm, how many μm is that?b. If I estimate that 9 of my specimen could fit across the field of view, how big is each one in mm, and μm?c. How big would the high power field of view be if the low power objective is 4x and the high power is 50x?

2 a. If the high power field of view is 425 μm, how many mm is that?

b. If I estimated that 2.5 specimens could fit across the field of view, how big is my specimen, in mm and μm?

c. If each specimen is 30 μm, and 10 can fit across, how big is the field of view?

d. If each specimen is 120 μm and the field of view is 630 mm, how many can fit across?