· web viewappendix 1. steps followed during preparation of permanent slides (slides a – m)...
TRANSCRIPT
![Page 1: · Web viewAppendix 1. Steps followed during preparation of permanent slides (Slides a – m) Slide a. Collected parasites in petridish Slide b. Relaxation of collected parasites](https://reader035.vdocuments.mx/reader035/viewer/2022070620/5e2e6b3fa043ac7ba93acb5d/html5/thumbnails/1.jpg)
Appendix 1. Steps followed during preparation of permanent slides (Slides a – m)
Slide a. Collected parasites in petridish
Slide b. Relaxation of collected parasites in physiological saline for 1 hr
![Page 2: · Web viewAppendix 1. Steps followed during preparation of permanent slides (Slides a – m) Slide a. Collected parasites in petridish Slide b. Relaxation of collected parasites](https://reader035.vdocuments.mx/reader035/viewer/2022070620/5e2e6b3fa043ac7ba93acb5d/html5/thumbnails/2.jpg)
Slide c. Fixation of parasites in 10% formalin
Slide d . Washing of parasites in running water for 6 hrs
![Page 3: · Web viewAppendix 1. Steps followed during preparation of permanent slides (Slides a – m) Slide a. Collected parasites in petridish Slide b. Relaxation of collected parasites](https://reader035.vdocuments.mx/reader035/viewer/2022070620/5e2e6b3fa043ac7ba93acb5d/html5/thumbnails/3.jpg)
Slide e. Staining the parasites with Borax Carmine for overnight
Slide f. De-stain the parasites in acid alcohol until the stain removed
![Page 4: · Web viewAppendix 1. Steps followed during preparation of permanent slides (Slides a – m) Slide a. Collected parasites in petridish Slide b. Relaxation of collected parasites](https://reader035.vdocuments.mx/reader035/viewer/2022070620/5e2e6b3fa043ac7ba93acb5d/html5/thumbnails/4.jpg)
Slide g. Dehydration in 90% ethanol for 15 minutes
Slide h. Dehydration in 95% ethanol for 15 minutes
![Page 5: · Web viewAppendix 1. Steps followed during preparation of permanent slides (Slides a – m) Slide a. Collected parasites in petridish Slide b. Relaxation of collected parasites](https://reader035.vdocuments.mx/reader035/viewer/2022070620/5e2e6b3fa043ac7ba93acb5d/html5/thumbnails/5.jpg)
Slide i. Dehydration in absolute alcohol for 30 minutes
Slide j. Dehydration in acid alcohol for 30 minutes
![Page 6: · Web viewAppendix 1. Steps followed during preparation of permanent slides (Slides a – m) Slide a. Collected parasites in petridish Slide b. Relaxation of collected parasites](https://reader035.vdocuments.mx/reader035/viewer/2022070620/5e2e6b3fa043ac7ba93acb5d/html5/thumbnails/6.jpg)
Slide k. Cleared in Lectophenol for 15 minutes
Slide l. Mounted in Canada Balsam Slide m. Prepared permanent slides