The most highly cited works in Web of Science as of January 2006

January2006

Year Author Publication Title Volume

282,778 1951LOWRY OH,ROSEBROUGHNJ, FARR ALL,RANDALL RJ

JOURNAL OFBIOLOGICALCHEMISTRY

PROTEIN MEASUREMENTWITH THE FOLIN PHENOLREAGENT

193

193,046 1970 LAEMMLI UK NATURE CLEAVAGE OFSTRUCTURAL PROTEINSDURING ASSEMBLY OFHEAD OFBACTERIOPHAGE-T4

227

117,410 1976 BRADFORD MM ANALYTICALBIOCHEMISTRY

RAPID AND SENSITIVEMETHOD FORQUANTITATION OFMICROGRAM QUANTITIESOF PROTEIN UTILIZINGPRINCIPLE OF PROTEIN-DYE BINDING

72

68,998 1989 SAMBROOK J MOLECULARCLONINGLABORATORYMANUAL

62,532 1977 SANGER F,NICKLEN S,COULSON AR

PROCEEDINGS OFTHE NATIONALACADEMY OFSCIENCES OF THE

DNA SEQUENCING WITHCHAIN-TERMINATINGINHIBITORS

74

UNITED STATES OFAMERICA

54,765 1987 CHOMCZYNSKIP, SACCHI N

ANALYTICALBIOCHEMISTRY

SINGLE-STEP METHOD OFRNA ISOLATION BY ACIDGUANIDINIUMTHIOCYNANATE PHENOLCHLOROFORMEXTRACTION

162

47,932 1979 TOWBIN H,STAEHELIN T,GORDON J

PROCEEDINGS OFTHE NATIONALACADEMY OFSCIENCES OF THEUNITED STATES OFAMERICA

ELECTROPHORETICTRANSFER OF PROTEINSFROM POLYACRYLAMIDEGELS TONITROCELLULOSE SHEETS- PROCEDURE AND SOMEAPPLICATIONS

76

36,371 1982 MANIATIS T MOLECULARCLONING

34,869 1957 FOLCH J, LEESM, STANLEYGHS

JOURNAL OFBIOLOGICALCHEMISTRY

A SIMPLE METHOD FORTHE ISOLATION ANDPURIFICATION OF TOTALLIPIDES FROM ANIMALTISSUES

226

33,861 1989 SAMBROOK J MOLECULARCLONINGLABORATORYMANUAL

31,067 1975 SOUTHERN EM JOURNAL OFMOLECULAR BIOLOGY

DETECTION OF SPECIFICSEQUENCES AMONG DNAFRAGMENTS SEPARATEDBY GEL-ELECTROPHORESIS

98

29,403 1994 AM PSYCHASSN

DIAGN STAT MANMENT

29,319 1958 KAPLAN EL,MEIER P

JOURNAL OF THEAMERICANSTATISTICALASSOCIATION

NONPARAMETRIC-ESTIMATION FROMINCOMPLETEOBSERVATIONS

53

23,269 1969 WEBER K,OSBORN M

JOURNAL OFBIOLOGICALCHEMISTRY

RELIABILITY OFMOLECULAR WEIGHTDETERMINATIONS BYDODECYL SULFATE-POLYACRYLAMINDE GELELECTROPHORESIS

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23,223 1959 BLIGH EG,DYER WJ

CANADIAN JOURNALOF BIOCHEMISTRYAND PHYSIOLOGY

A RAPID METHOD OFTOTAL LIBID EXTRACTIONAND PURIFICATION

37

22,976 1962 MURASHIGE T,SKOOG F

PHYSIOLOGIAPLANTARUM

A REVISED MEDIUM FORRAPID GROWTH AND BIOASSAYS WITH TOBACCOTISSUE CULTURES

15

22,918 1979 CHIRGWIN JM,PRZYBLYLA AE,MACDONALDRJ, RUTTER WJ

BIOCHEMISTRY ISOLATION OFBIOLOGICALLY-ACTIVERIBONUCLEIC-ACID FROMSOURCES ENRICHED INRIBONUCLEASE

18

22,565 1963 REYNOLDS ES JOURNAL OF CELLBIOLOGY

USE OF LEAD CITRATE ATHIGH PH AS ANELECTRON-OPAQUESTAIN IN ELECTRONMICROSCOPY

17

22,407 1949 SCATCHARD G ANNALS OF THE NEWYORK ACADEMY OFSCIENCES

THE ATTRACTIONS OFPROTEINS FOR SMALLMOLECULES AND IONS

51

22,375 1956 SIEGEL S NONPARAMETRICSTATISTICS

21,326 1964 DAVIS BJ ANNALS OF THE NEWYORK ACADEMY OFSCIENCES

DISCELECTROPHORESIS.2.METHOD ANDAPPLICATION TO HUMANSERUM PROTEINS

121

21,300 1925 FISKE CH JOURNAL OFBIOLOGICALCHEMISTRY

66

20,992 1983 FEINBERG AP,VOGELSTEIN B

ANALYTICALBIOCHEMISTRY

A TECHNIQUE FORRADIOLABELING DNARESTRICTIONENDONUCLEASEFRAGMENTS TO HIGHSPECIFIC ACTIVITY

132

20,738 1972 COX DR J ROY STAT SOC BMET

34

20,715 1987 AM PSYCHASSN

DIAGN STAT MANMENT

19,709 1990 ALTSCHUL SF,GISH W,MILLER W,MYERS EW,LIPMAN DJ

JOURNAL OFMOLECULAR BIOLOGY

BASIC LOCAL ALIGNMENTSEARCH TOOL

215

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19,107 1981 SOKAL RR,ROHLF FJ

BIOMETRY

19,054 1956 DUBOIS M,GILLES KA,HAMILTON JK,REBERS PA,SMITH F

ANALYTICALCHEMISTRY

COLORIMETRIC METHODFOR DETERMINATION OFSUGARS AND RELATEDSUBSTANCES

28

18,827 1967 SNEDECORGW,COCHRAN, WG

STATISTICALMETHODS

18,254 1975 FOLSTEIN MF,FOLSTEIN SE,MCHUGH PR

JOURNAL OFPSYCHIATRICRESEARCH

MINI-MENTAL STATE -PRACTICAL METHOD FORGRADING COGNITIVESTATE OF PATIENTS FORCLINICIAN

12

17,501 1994 THOMPSONJD, HIGGINS,GD, GIBSONTJ

NUCLEIC ACIDSRESEARCH

CLUSTAL-W - IMPROVINGTHE SENSITIVITY OFPROGRESSIVE MULTIPLESEQUENCE ALIGNMENTTHROUGH SEQUENCEWEIGHTING, POSITION-SPECIFIC GAP PENALTIESAND WEIGHT MATRIXCHOICE

22

17,093 1986 PAXINOS G,WATSON C

THE RAT BRAIN INSTEREOTAXICCOORDINATES

16,801 1975 OFARRELL PH JOURNAL OFBIOLOGICALCHEMISTRY

HIGH-RESOLUTION 2-DIMENSIONALELECTROPHORESIS OFPROTEINS

250

16,748 1956 BURTON K BIOCHEMICALJOURNAL

STUDY OF THECONDITIONS ANDMECHANISM OF THEDIPHENYLAMINEREACTION FOR THECHOLORIMETRICESTIMATION OFDEOXYRIBONUCLEIC

62

ACID

15,998 1985 GRYNKIEWICZG, POENIE M,TSIEN RY

JOURNAL OFBIOLOGICALCHEMISTRY

A NEW GENERATION OFCA-2+ INDICATORS WITHGREATLY IMPROVEDFLUORESCENCEPROPERTIES

260

15,686 1997 ALTSCHULl SF,MADDEN TL,SCHAFFER AA,ZHANG JH,ZHANG Z,MILLER W,LIPMAN DJ

NUCLEIC ACIDSRESEARCH GAPPED BLAST AND PSI-

BLAST; A NEWGENERATION OFPROTEIN DATABASESEARCH PROGRAMS

25

15,387 1993BECKE AD

JOURNAL OFCHEMICAL PHYSICS

DENSITY-FUNCTIONALTHERMOCHEMISTRY .3.THE ROLE OF EXACTEXCHANGE

98

15,164 1960 STEEL RGD, JHTORRIE

PRINCIPLES ANDPROCEDURES OFSTATISTICS

January Author Publication Title Volume2006

Year

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15,013 1972 MILLER JH EXPERIMENTS INMOLECULARGENETICS

14,880 1974 INTERNATIONALTABLES FOR XRAYCRYSTALLOGRAPHY

4

14,820 1981 HAMILL OP,MARTY A,NEWHER E,SAKMANN B,SIGWORTH FJ

PFLUGERS ARCHIV-EUROPEAN JOURNALOF PHYSIOLOGY

IMPROVED PATCH-CLAMPTECHNIQUES FOR HIGH-RESOLUTION CURRENTRECORDING FROM CELLSAND CELL-FREEMEMBRANE PATCHES

391

14,813 1949 GORNALL AG,BARDAWILLCY, DAVID MM

JOURNAL OFBIOLOGICALCHEMISTRY

DETERMINATION OFSERUM PROTEINS BYMEANS OF THE BIURETREACTION

177

14,718 1968 BOYUM A SCANDINAVIANJOURNAL OFCLINICAL &LABORATORYINVESTIGATION

ISOLATION OFMONONUCLEAR CELLSAND GRANULOCYTESFROM HUMAN BLOOD-ISOLATION OFMONONUCLEAR CELLS BYONE CENTRIFUGATIONAND OF GRANULOCYTESBY COMBININGCENTRIFUGATION ANDSEDIMENTATION AT LG

21

14,604 1988 LEE CT, YANGWT, PARR RG

PHYSICAL REVIEW B DEVELOPMENT OF THECOLLE-SALVETTICORRELATION-ENERGYFORMULA INTO AFUNCTIONAL OF THEELECTRON-DENSITY

37

14,546 1984 ZAR, JH BIOSTATISTICALANALYSIS

14,438 1982 MANIATIS T MOLECULARCLONINGLABORATORYMANUAL

14,413 1976 SHANNON RD ACTACRYSTALLOGRAPHICASECTION A

REVISED EFFECTIVEIONIC-RADII ANDSYSTEMATIC STUDIES OFINTERATOMIC DISTANCESIN HALIDES AND

32

CHALCOGENIDES

14,308 1980 MAXAM AM,GILBERT W

METHODS INENZYMOLOGY

SEQUENCING END-LABELED DNA WITHBASE-SPECIFICCHEMICAL CLEAVAGES

65

14,014 1984 DEVEREUX J,HAEBERLI P,SMITHIES O

NUCLEIC ACIDSRESEARCH

A COMPREHENSIVE SETOF SEQUENCE-ANALYSESPROGRAMS FOR THE VAX

12

13,947 1988 SAIKI RK,GELFAND DH,STOFFEL S,SCHARF SJ,HIGUCHI R,HORN GT,MULLIS KB,ERLICH HA

SCIENCE PRIMER-DIRECTEDENZYMATICAMPLIFICATION OF DNAWITH A THERMOSTABLEDNA-POLYMERASE

239

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The most highly cited works in Web of Science as of January 2006 (continued - Page 4)