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  • Genotipos/Dinmica: EC70124 EC70124

    P-S6 (S235/236)P-Akt(S473)P-Akt(T308)P-p65 (S536)P-Stat3 (T705)P-PDK1 (S241)GAPDHwt0,511,50,511,5myrAkt;Trp53DCE

    Trp53DCEmyrAkt

    00ba0011

    69.psd

    70.psd

    71.psd

    72.psd

    73.psd

    74.psd

    75.psd

    76.psd

    77.psd

    78.psd

    79.psd

    80.psd

    81.psd

    82.psd

  • Genotipos/Dinmica: Prodigiosina 6h

    P-S6 (S235/236)P-Akt(S473)P-Akt(T308)P-p65 (S536)P-Stat3 (Y705)P-PDK1 (S241)GAPDHTrp53DCEmyrAktwt15101510myrAkt;Trp53DCE

    00ba0055

    83.psd

    84.psd

    85.psd

    86.psd

    87.psd

    88.psd

    89.psd

    90.psd

    91.psd

    92.psd

    93.psd

    94.psd

    95.psd

    96.psd

  • a

    LC3 IICaspasa 3 actGAPDHwtTrp53DCEmyrAkt-+-+-+-+myrAkt;Trp53DCEwtTrp53DCEmyrAkt-+-+-+-+myrAkt;Trp53DCEba

    Caspasa 3 actGAPDH15151010wtmyrAkt;Trp53DCE0,510,511,51,5wtmyrAkt;Trp53DCEba0000

    97.psd

  • Tumores tratados con EC70124ACTINAP-PDK1P-Akt(S473)CTRL+EC70124P-Akt(T308)LC3bP-S6Cycl BP-p65P-Stat3AKT total?

    P-S6 (S235/236)P-Akt(S473)P-Akt(T308)P-p65 (S536)P-Stat3 (T705)P-PDK1 (S241)GAPDH+Vehculo+EC70124 15591563302117093378355635603564337417253370171317173552+Vehculo+Prodigiosina 9801980499572039923995499209836200980499179926IPIVba

    107.psd

    Grfico1

    150.796447372392.6597045213139.486713819456.69946421275.9218224618127.234502470455.396750458371.994831644855.4176944093

    255.1831936707176.976386152296.275154066847.105040247978.5398163397209.439510239325.656340004336.291678334383.7758040957

    316.0505041364160.849543863870.958106069152.072945430868.9186888381193.019452636623.090706003929.389075675695.0331777711

    634.9944151068230.9070600389430.406571122286.3644764423116.037866253178.128303458527.95546222844.888646630891.8654051787

    855.2985999398603.4371169015509.6495806176184.7256480311356.6807219253158.524765300116.604887970552.6468096889155.8622655262

    983.2923206348855.015856601740.6786391711468.0721726436549.1017011614207.921073790116.604887970579.2854209962196.9354478793

    1642.00576027631153.5928223982858.8328916751412.1643897804709.5810606908354.22085487768.181230868745.5023043958340.0978251033

    2061.67017891831432.63327068021317.0017170771318.2621853646796.3282878381392.54200206616.604887970516.6211195326513.1791599639

    1559

    1563

    1725

    1721

    1717

    1709

    1733

    1729

    1713

    EC_IP.11

    Animal(b)(h)(V)V paperFechaObservaciones

    NA??00

    NA68151144NO tratado

    NA7.48.92552441/27/12(Lo "encontr" de ese tamao)

    NA8.19.23163021/31/12

    NA10.511.06356062/2/12

    NA11.013.58558172/5/12

    NA11.514.29839392/8/12

    NA14.016.0164215682/12/12

    NA15.017.5206219692/15/12

    NA16.518.5263725182/18/12

    RIP

    2D3.03.014141/27/12

    2D3.03.516161/31/12

    2D5.36.393882/2/12NO tratado

    2D6.58.01771692/5/12

    2D6.47.51611542/8/12

    2D7.09.02312212/12/12

    2D9.812.06035762/15/12

    2D10.814.08558162/18/12

    2D12.015.3115411022/21/12

    12.816.714331368RIP

    2I-TNDND001/27/12

    2I-TNDND001/31/12

    2I-TNDND002/2/12

    2I-TNDND002/5/12

    2I-T3.03.014142/8/12

    2I-T3.54.026252/12/1203691215182124

    2I-T4.84.757542/15/12EC!

    2I-T4.25.147452/18/12

    2I-T4.64.752502/21/12

    2I-T5.26.186822/24/12

    2I-T7.07.21851762/27/12

    2I-T9.69.74684473/1/12

    2I-T8.710.44123943/4/12

    2I-T7.411.13183043/7/12

    2D-TNDND001/27/12

    2D-TNDND001/31/12

    2D-T3.03.014142/2/12

    2D-T3.54.630282/5/12

    2D-T4.25.652492/8/12

    2D-T5.05.876732/12/12EC!

    2D-T5.06.079752/15/12

    2D-T4.56.569662/18/12

    2D-T5.47.61161112/21/12

    2D-T8.79.03573412/24/12

    2D-T9.910.75495242/27/12

    2D-T11.011.27106783/1/12

    2D-T11.511.57967603/4/12

    1I-TNDND001/27/12

    1I-TNDND001/31/12

    1I-TNDND002/2/12

    1I-TNDND002/5/12

    1I-T9.03.01271222/8/12EC!

    1I-T10.04.02092002/12/12(pequeo pero alargado)

    1I-T9.64.01931842/15/12

    1I-T9.04.21781702/18/12

    1I-T8.74.01591512/21/12

    1I-T9.54.42081992/24/12

    1I-T10.85.83543382/27/12

    1I-T10.56.83933753/1/12

    RIP

    2INDND001/27/12

    2INDND001/31/12

    2INDND002/2/12

    2INDND002/5/12

    2I3.74.230292/8/12

    2I4.26.055532/12/12NO tratado

    2I5.06.484802/15/12

    2I5.56.095912/18/12

    2I5.55.892882/21/12

    2I6.37.51561492/24/12

    2I6.97.91971882/27/12

    2I8.19.93403253/1/12

    2I9.012.15134903/4/12

    1D-TNDND001/27/12

    1D-TNDND001/31/12

    1D-TNDND002/2/12

    1D-TNDND002/5/12

    1D-TNDND002/8/12

    1D-T3.04.019182/12/12

    1D-T4.65.055532/15/12EC!

    1D-T3.54.026252/18/12

    1D-T3.53.623222/21/12

    1D-T3.73.928272/24/12

    1D-T3.13.317162/27/12

    1D-T3.13.317163/1/12

    1D-T2.52.5883/4/12

    1D-T3.13.317163/7/12(No hay tumor, solo bolita de matrigel!)

    1DNDND001/27/12

    1DNDND001/31/12

    1DNDND002/2/12

    1DNDND002/5/12

    1DNDND002/8/12

    1D3.24.323222/12/12

    1D6.07.41391332/15/12NO tratado

    1D5.26.896922/18/12

    1D4.47.071682/21/12

    1D10.47.64304112/24/12(Se alarga, se fusiona otro tumor?)

    1D11.17.95104872/27/12

    1D12.49.27417073/1/12

    1D13.59.08598203/4/12

    1D14.611.8131712583/7/12

    NA-TNDND001/27/12

    NA-TNDND001/31/12

    NA-TNDND002/2/12

    NA-TNDND002/5/12

    NA-TNDND002/8/12

    NA-T3.03.516162/12/12

    NA-T5.05.572692/15/12EC!

    NA-T3.84.836352/18/12

    NA-T3.74.129282/21/12

    NA-T4.15.145432/24/12

    NA-T4.25.753502/27/12

    NA-T5.25.679763/1/12

    NA-T4.34.746433/4/12

    NA-T3.23.117163/7/12(No hay tumor, solo bolita de matrigel!)

    EC_IP.11

    EC_IP.12

    Inyeccin Kc. Orales DB(10nu/un): 26.04.2012Tratamiento IP EC70124

    Animal(b)(h)(V)V paperFechaObservaciones

    N/AN/A006/5/12

    N/A4434326/8/12

    N/A5.04.052506/11/12Tratados ECMediaSDSE

    N/A7.54.01181136/14/12EC!112.5126102.851081129.9252.7526997238

    N/A7.84.71501436/17/12142.974171.5150.28171.515914.66691227454.0678695583

    N/A7.04.0103986/20/1298159.25115.43472.611136.473143964710.1158300571

    N/A8.05.51841766/23/12176164.28134.09687.46414039.494980744410.9539367844

    N/A9.05.52332236/26/12222.75208138.72113.417152.935500611614.6816662883

    N/A9.55.02362266/29/12225.625317.4166.6151.2321575.294180316820.8828482982

    N/A7.35.51531477/2/12146.5475372.438168.75166.6214106.373664441629.5027462695

    N/A8.05.01681607/5/12160421.35152.1196.875233127.349711056835.3204548562

    N/A8.55.01891817/8/12180.625476.672228.15223.8925277134.614670696537.3353921251

    N/A9.05.82462357/11/12234.9648208361363201.381567759655.8531975762

    N/A10.06.03143007/14/12300911.25364.5496.125518274.596870714676.1594690341

    N/A10.86.64033857/17/12384.9121090.936482.7095625646312.644487279486.7119792213

    N/A10.98.04984757/20/12475.241201.25976.5625760.5853309.73322873285.9045413775

    N/A11.79.46746437/23/12

    M1I4.54.548466/5/12126272147126MediaSD

    M1I6.07.01321266/8/12EC!126320171.519620382.983306554223.0154282145

    M1I7.07.01801726/11/12171.5405224260.1265100.07858578827.7568055873

    M1I7.06.51671596/14/12196.875358.9335242.064316.8427972.868830394520.2101772593

    M1I7.46.01721646/17/12359.72475383.5625720485164.629794138945.6600895567

    M1I8.06.52182086/20/12424.175818.62561.8684622168.584406671946.7569017307

    M1I9.27.53323176/23/12561.81008.0625612.36756735200.035120774455.4797603727

    M1I9.97.63903726/26/12672.81285.47870.25820.3125912262.58076077872.8267997643

    M1I10.67.54414216/29/12820.31251681.758641002.3751,092400.6809840466111.1289102372

    M1I11.27.64994777/2/12765.6252009.61211.96251095.031,271527.6943318515146.3560747125

    M1I12.09.06796487/5/121093.5137210781,181165.4481288345.8870547635

    M1I13.510.09549117/8/1213231681.751685.071,563208.089431815657.7136243303

    M1I15.49.2114210917/11/121512.632021.251,767359.648651047199.748588654

    M1I15.510.0125812017/14/12

    M1I17.013.0196718797/17/12

    7/20/12RIP

    M1DN/A006/5/12

    M1DN/A006/8/12

    M1D3.03.014146/11/12

    M1D4.54.548466/14/12

    M1D5.56.81081036/17/12EC!

    M1D6.86.51571506/20/12

    M1D6.65.31211156/23/12

    M1D6.85.81401346/26/12

    M1D6.86.01451396/29/12

    M1D7.06.81741677/2/12

    M1D7.56.01771697/5/12

    M1D7.85.01591527/8/12

    M1D7.87.52392287/11/12

    M1D8.06.52182087/14/12

    M1D9.09.03823657/17/12

    M1D10.39.15054837/20/12

    M1D12.512.510239777/23/12

    A1D4.54.548466/5/12

    A1D6.06.01131086/8/12EC!

    A1D7.07.01801726/11/12

    A1D5.54.876736/14/12

    A1D5.85.292876/17/12

    A1D6.06.31191136/20/12

    A1D7.16.01581516/23/12

    A1D7.06.81741676/26/12

    A1D7.57.02061976/29/12

    A1D8.36.52342247/2/12

    A1D9.58.03783617/5/12

    A1D10.59.05204967/8/12

    A1D12.58.06546257/11/12RIP

    A1D13.09.07967617/14/12

    A1D15.013.0153214637/17/12

    A1D

    2MI6.07.01321266/5/12NO tratado

    2MI6.07.01321266/8/12

    2MI7.07.01801726/11/12

    2MI7.57.02061976/14/12

    2MI9.28.53773606/17/12

    2MI9.59.44444246/20/12

    2MI10.610.05885626/23/12

    2MI11.610.07056736/26/12

    2MI12.510.58598206/29/12

    2MI12.59.88027667/2/12

    2MI13.512.0114510947/5/12

    2MI14.013.5138513237/8/12

    2MI14.714.0158415137/11/12

    2MI16.017.3231922147/14/12RIP

    2MD8.08.52852726/5/12NO tratado

    2MD8.010.03353206/8/12

    2MD9.010.04244056/11/12

    2MD9.38.33763596/14/12

    2MD10.09.54974756/17/12

    2MD12.211.08578196/20/12

    2MD12.712.5105610086/23/12

    2MD13.813.5134612856/26/12

    2MD15.514.0176116826/29/12