Vitamin C Content

By Ramanan Sivasundaram and James

Shevelin

Prelab

• Vitamin C is required for good health and it prevents SCURVY

Vitamin C (C6H8O6)also prevents potentially harmful reactions that are involved in the transfer of electrons.Chemical Equation for the Titration: C6H8O6 + I2 C6H6O6 + 2I + 2H⁺ �

Well, What's the Problem?

• Of Simply Apple, Orange, and Lemonade, in what order, from least to greatest, is the Vitamin C content?

Hypothesis: 1. Simply Orange, 2. Simply Lemonade, 3. Simply Apple

Materials

• Simply Lemonade, Apple, and Orange

• 250 mL Erlenmeyer Flask• Iodine• Starch Solution• Buret• Distilled H2O

• Electronic Scale• Pipet

Procedure

1. Pour 50 mL of juice into 250 mL Erlenmeyer flask.

2. Add 15 drops of starch indicator solution.3. Dissolve in 25 mL of Distilled H2O

4. Record initial level reading of iodine solution in the buret.

5. Add iodine solution from the buret to the Vitamin C solution until a deep-blue color is obtained, or any color change is obtained.

6. Record the final level reading of the iodine solution in the buret.

7. Repeat for Trial 2

Data Table (Trial 1)

Simply Apple

Simply Orange

Simply Lemonade

Initial Level Reading of Iodine Solution

.3 mL 5.9 mL 33.2 mL

Final level Reading of Iodine Solution

2.8 mL 33.2 mL 34.9 mL

Data Table (Trial 2)

Simply Apple

Simply Orange

Simply Lemonade

Initial Level Reading of Iodine Solution

2.8 mL 1.2 mL 34.9 mL

Final level Reading of Iodine Solution

5.9 mL 28.7 mL 36.3 mL

Results TableJuice Trial Vitamin C

Simply Lemonade 1 1.8 mg

Simply Orange 1 29.5 mg

Simply Apple 1 2.7 mg

Simply Lemonade 2 1.5mg

Simply Orange 2 29.7 mg

Simply Apple 2 3.4 mg

Conclusion

• Our hypothesis stated that Simply Orange would have the most Vitamin C, with lemonade and apple following, but after our trials, we have come to the conclusion that Simply Orange has the most Vitamin C with apple and lemonade following. Lemonade had about 1.65 mg of Vitamin C while Orange juice had 29.6 mg of Vitamin C and Apple juice had 3.05 mg of Vitamin C in a 50 mL sample of each juice.

• Some sources of error are the color change and the method of stirring the solution. Determining a color change varies per person, which means that the amount of Iodine let out of the buret is not exact, since each group will let out a different amount based on their decision of a color change. If the solution is not stirred well enough, then the starch solution and the iodine will not dilute into the whole solution, causing the user to disperse more Iodine than needed into the juice.

Conclusion pt. 2

• More of the starch solution could have been added to the juice, so the amount of Iodine used could be more exact.

• A mechanical stirrer could be used so that the stirring would be more efficient and the juice, starch, and Iodine would all be mixed evenly.

• A greater amount of trials would make the results more accurate.

• 101 mL of Simply Orange, 1,800 mL of Simply Lemonade, and 1,000 mL of Simply Apple would fufill the U.S. RDA’s requirement of Vitamin C intake.