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Page 1: Vinod Kumar, Ph.D. - University of Nebraska Medical … Kumar, Ph.D. 1. Address (Laboratory) Permanent Postdoctoral Research Associate P. H. C. Babhanjot, (Post) Department of Pharmaceutical

Vinod Kumar, Ph.D.

1. Address (Laboratory) Permanent

Postdoctoral Research Associate P. H. C. Babhanjot, (Post)

Department of Pharmaceutical Sciences Gaurachowki Market

University of Nebraska Medical Center Gonda (District)

Omaha, Nebraska 68198 Uttar Pradesh, India 271312

Tel (cell): (402)906-9876

E-mail: [email protected]

2. Education

Ph.D. (Biochemistry) CFTRI, Mysore (India) 2009-2014

Master’s (Biotechnology) University of Mysore (India) 2004-2006

Bachelors (Zoology, Botany and Chemistry) University of Lucknow (India) 1999-2002

3. Professional Appointments

University of Nebraska Medical Center Omaha, NE (USA)

Postdoctoral Research Associate Dec 2014-present

4. Honors and Awards

UNeMed Research and Innovation Recognition Award

Nebraska Medical Center Omaha, (NE) USA 2017

Senior Research Fellowship (SRF), Government of India 2009-2012

Best poster presentation (2nd) 2012

Selected for student oral presentation 2011

Best poster presentation award 2007

Graduate Aptitude Test for Engineering (Life Science) , Government of India 2006

CSIR & UGC-National Eligibility Test, Government of India 2005

All India combined Biotechnology Entrance Examination, Government of India 2004

5. Technical Expertise

Cancer biology, cell culture techniques: Maintenance and handling of human pancreatic cancer

cell lines (MiaPaCa-2, Hpaff-2, BxPC3, PANC-1, HPDE), medulloblastoma cell lines (Daoy, HDMB03),

liver cell lines (HSC, LX2), human lens epithelial cell lines, Cell viability assay, ROS assay, Cell cycle,

Apoptosis, Clonogenic, Invasion, Migration, IHC, generation of stable and resistance cell lines, stem

cell, transfection (siRNA, miRNA), miRNA profiling, RNA sequencing etc.

Animal handling: Maintenance and handling of NSG mice, generation of sub-cutaneous and

orthotopic medulloblastoma tumor in mice, stereotaxic injection in brain, i. v. injection, in vivo, in vitro,

and ex-vivo bioluminescence imaging, general surgery of mice.

Molecular biology techniques: DNA and RNA isolation, Primer designing, cDNA synthesis, PCR and

Agarose gel electrophoresis, Real-Time PCR, cloning, competent cell preparation, transformation,

restriction digestion, ligation, blue/white colony screening, plasmid isolation, DNA sequencing,

recombinant protein expression (E. coli), site-directed mutagenesis etc.

Protein biology techniques: Isolation and purification of proteins by chromatographic techniques

(HPLC, FPLC, affinity, ion-exchange, size-exclusion chromatography), SDS-PAGE and Native PAGE,

Western blot, heterologous protein expression, protein sequencing, enzyme kinetics, spectroscopy,

ESI-MS, circular dichroism (CD), fluorescence spectroscopy, differential scanning calorimetry (DSC)

etc.

Computer and Bioinformatics: Protein modeling (ExPASy, PyMOL), multiple and pair wise alignments,

phylogenetic tree analysis, secondary structure prediction, MS office, data interpretation and

manuscript writing.

Page 2: Vinod Kumar, Ph.D. - University of Nebraska Medical … Kumar, Ph.D. 1. Address (Laboratory) Permanent Postdoctoral Research Associate P. H. C. Babhanjot, (Post) Department of Pharmaceutical

6. Professional Research Experience

Postdoctoral at Department of Pharmaceutical Sciences, University of Nebraska Medical Center,

(March 1, 2016- to date). Working on a project entitled “micro RNA and Hedgehog inhibitor-

based therapy for treatment of Pancreatic Cancer and Medulloblastoma. (Mentor: Prof Ram I

Mahato).

Postdoctoral at Department of Ophthalmology and Visual Sciences, University of Nebraska

Medical Center, Omaha, Nebraska, USA (December 9, 2014, to February 28, 2016). Worked on a

project entitled “Role of epigenetic and Sumoylation signaling on cellular homeostasis during

oxidative stress and aging with special reference to cell survival molecules, Prdx6 and LEDGF in

human lens epithelial cells.” (Mentor: Prof D. P. Singh).

Ph.D. in Biochemistry (2009-2014) from Council of Scientific and Industrial Research (CSIR)-Central

Food Technological Research Institute (CFTRI), Mysore, INDIA. Title of the Ph.D. thesis: “Molecular

Engineering of the horsegram (Dolichos biflorus) Seed Bowman-Birk Inhibitor: Implications of the

disulfide framework on functionality.” (Mentor: Dr. Lalitha R. Gowda).

Project Assistant at Department of Protein Chemistry and Technology, CSIR-CFTRI, Mysore.

(August 2006 to June 2009). Worked on the project entitled “Cyclic peptide as proteinase

inhibitor: purification, molecular cloning, and expression.” (Mentor: Dr. Lalitha R. Gowda).

Project work for master's programme at Department of Biophysics, All India Institute of Medical

Sciences, New Delhi, INDIA (January 2006 to April 2006). Worked on “Isolation, Purification, and

Crystallization of C-lobe of Bovine Lactoferrin.” (Mentor: Prof. T. P. Singh).

7. Publications

Journal Publications:

1. Vinod Kumar, Amit K Chaudhary, Yuxiang Dong, Haizhen A. Zhong, Goutam Mondal, Feng Lin,

Virender Kumar, and Ram I Mahato. Design, Synthesis and Biological Evaluation of novel

Hedgehog inhibitors for treating Pancreatic Cancer. Scientific Reports. 2017, 7:1665.

2. Vinod Kumar, Virender Kumar, Timothy McGuire, Donald W. Coulter, John G. Sharp and Ram I.

Mahato. Challenges and recent advances in medulloblastoma therapy. Trends in

Pharmacological Sciences. 2017, 38: 1061-1084.

3. Vinod Kumar, Saravanan Murugeson, Neha Vithani, Balaji Prakash and Lalitha R Gowda. A salt

bridge stabilized C-terminal hook is critical for the dimerization of a Bowman-Birk inhibitor.

Archives of Biochemistry and Biophysics. 566 (2015) pp 15-25.

4. Vinod Kumar, and Lalitha R. Gowda. The contribution of two disulfide bonds in the trypsin binding

domain of horsegram (Dolichos biflorus) Bowman-Birk inhibitor to thermal stability and

functionality. Archives of Biochemistry and Biophysics. 537 (2013), pp 49-61.

5. Vinod Kumar, and Lalitha R. Gowda. Production of horsegram (Dolichos biflorus) Bowman-Birk

Inhibitor by an intein mediated protein purification system. Protein Expression and Purification. 89

(2013) pp 16-24.

6. Vinod Kumar, Virender Kumar, Amit Kumar Chaudhary, Donald W. Coulter, Timothy McGuire,

and Ram I. Mahato. Impact of miRNA-mRNA Profiling and Their Correlation on Medulloblastoma

Tumorigenesis. Nat Comm (Under Review).

8. Poster/Oral Presentations

1. Design, Synthesis and Biological Evaluation of Potent Analogues of GDC-0449 for the Treatment

of Pancreatic Cancer. 4th Biopharmaceutical Research and Development Symposium at UNMC,

NE (USA). Oral presentation, 2017

2. Multi-pronged Treatment Strategies for Medulloblastoma. 15th Annual Pediatric Research Forum

at Children Hospital and Medical Center, Omaha, NE (USA). Poster, 2017

Page 3: Vinod Kumar, Ph.D. - University of Nebraska Medical … Kumar, Ph.D. 1. Address (Laboratory) Permanent Postdoctoral Research Associate P. H. C. Babhanjot, (Post) Department of Pharmaceutical

3. Synthesis and Nano-formulation of Potent Analogues of GDC-0449, a Hedgehog inhibitor for the

treatment of Pancreatic Cancer. 3rd Biopharmaceutical Research and Development Symposium

at UNMC, NE (USA). Poster, 2016

4. Association of Microbiologists of INDIA, Mysore chapter, CFTRI campus. Oral, 2014

5. Dimerization of Horsegram Bowman Birk Inhibitor is guided by an intramolecular interaction in the

monomer”. Recent Advances in computational Drug Design at IISc, India. Poster, 2013

6. Horsegram Bowman-Birk inhibitor: The contribution of interdomain disulfides to thermal and

proteolytic stability”. XXII ICFoST Meet CFTRI, India. Poster, 2012

(Note: Received second best poster presentation award)

7. Molecular engineering of Bowman-Birk inhibitor (BBI) of horsegram (Dolichos biflorus) seed: Effect

of interdomain disulfide bond deletion”. 80th Annual meeting of Society of Biological Chemists at

CIMAP, INDIA. Selected for oral presentation, 2011

8. Facile production of functional recombinant Bowman-Birk Inhibitor (BBI) using Ssp dnaB derived

self cleaving mini intein tag. Recent Trends in Biophysics at BHU, India. Poster 2010

9. Functional expression of horse gram (Dolichos biflorus) Bowman- Birk inhibitor deletion mutant

using modified self- splicing inteins. 77th Annual session & symposium of The National Academy of

Sciences (NASI), INDIA. Poster, 2007

(Note: Received best poster presentation award).

10 International symposium on Cardiovascular Diseases, Stroke and Diabetes, at AIIMS, New Delhi,

India. 2006

9. Workshops Attended

1. Bioinformatics in Genomics, Proteomics and Metabolomics at Indian Institute of Technology,

Kharagpur, India September 23-24, 2011

2. Emerging Techniques of Biophysics at Banaras Hindu University (BHU), Varanasi, INDIA.

February 13-16, 2010

10. References

Dr. Ram I. Mahato, Ph.D.

Professor and Chair,

Department of Pharmaceutical Sciences

University of Nebraska Medical Center, Omaha NE 68198 Email: [email protected]; Tel (office): (402) 559-5422

Dr. Kishore B. Challagundla, Ph.D.

Assistant Professor,

Department of Biochemistry and Molecular Biology

University of Nebraska Medical Center, Omaha NE 68198

Email: [email protected]; Tel (office): (402) 559-3441

Dr. Lalitha R. Gowda, Ph.D.

Chief Scientist (Retd),

Department of Protein Chemistry and Technology

Central Food Technological Research Institute (CFTRI), Mysore, India

E-mail: [email protected]; Tel (cell): +91-9482717138