vinod kumar, ph.d. - university of nebraska medical … kumar, ph.d. 1. address (laboratory)...
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Vinod Kumar, Ph.D.
1. Address (Laboratory) Permanent
Postdoctoral Research Associate P. H. C. Babhanjot, (Post)
Department of Pharmaceutical Sciences Gaurachowki Market
University of Nebraska Medical Center Gonda (District)
Omaha, Nebraska 68198 Uttar Pradesh, India 271312
Tel (cell): (402)906-9876
E-mail: [email protected]
2. Education
Ph.D. (Biochemistry) CFTRI, Mysore (India) 2009-2014
Master’s (Biotechnology) University of Mysore (India) 2004-2006
Bachelors (Zoology, Botany and Chemistry) University of Lucknow (India) 1999-2002
3. Professional Appointments
University of Nebraska Medical Center Omaha, NE (USA)
Postdoctoral Research Associate Dec 2014-present
4. Honors and Awards
UNeMed Research and Innovation Recognition Award
Nebraska Medical Center Omaha, (NE) USA 2017
Senior Research Fellowship (SRF), Government of India 2009-2012
Best poster presentation (2nd) 2012
Selected for student oral presentation 2011
Best poster presentation award 2007
Graduate Aptitude Test for Engineering (Life Science) , Government of India 2006
CSIR & UGC-National Eligibility Test, Government of India 2005
All India combined Biotechnology Entrance Examination, Government of India 2004
5. Technical Expertise
Cancer biology, cell culture techniques: Maintenance and handling of human pancreatic cancer
cell lines (MiaPaCa-2, Hpaff-2, BxPC3, PANC-1, HPDE), medulloblastoma cell lines (Daoy, HDMB03),
liver cell lines (HSC, LX2), human lens epithelial cell lines, Cell viability assay, ROS assay, Cell cycle,
Apoptosis, Clonogenic, Invasion, Migration, IHC, generation of stable and resistance cell lines, stem
cell, transfection (siRNA, miRNA), miRNA profiling, RNA sequencing etc.
Animal handling: Maintenance and handling of NSG mice, generation of sub-cutaneous and
orthotopic medulloblastoma tumor in mice, stereotaxic injection in brain, i. v. injection, in vivo, in vitro,
and ex-vivo bioluminescence imaging, general surgery of mice.
Molecular biology techniques: DNA and RNA isolation, Primer designing, cDNA synthesis, PCR and
Agarose gel electrophoresis, Real-Time PCR, cloning, competent cell preparation, transformation,
restriction digestion, ligation, blue/white colony screening, plasmid isolation, DNA sequencing,
recombinant protein expression (E. coli), site-directed mutagenesis etc.
Protein biology techniques: Isolation and purification of proteins by chromatographic techniques
(HPLC, FPLC, affinity, ion-exchange, size-exclusion chromatography), SDS-PAGE and Native PAGE,
Western blot, heterologous protein expression, protein sequencing, enzyme kinetics, spectroscopy,
ESI-MS, circular dichroism (CD), fluorescence spectroscopy, differential scanning calorimetry (DSC)
etc.
Computer and Bioinformatics: Protein modeling (ExPASy, PyMOL), multiple and pair wise alignments,
phylogenetic tree analysis, secondary structure prediction, MS office, data interpretation and
manuscript writing.
6. Professional Research Experience
Postdoctoral at Department of Pharmaceutical Sciences, University of Nebraska Medical Center,
(March 1, 2016- to date). Working on a project entitled “micro RNA and Hedgehog inhibitor-
based therapy for treatment of Pancreatic Cancer and Medulloblastoma. (Mentor: Prof Ram I
Mahato).
Postdoctoral at Department of Ophthalmology and Visual Sciences, University of Nebraska
Medical Center, Omaha, Nebraska, USA (December 9, 2014, to February 28, 2016). Worked on a
project entitled “Role of epigenetic and Sumoylation signaling on cellular homeostasis during
oxidative stress and aging with special reference to cell survival molecules, Prdx6 and LEDGF in
human lens epithelial cells.” (Mentor: Prof D. P. Singh).
Ph.D. in Biochemistry (2009-2014) from Council of Scientific and Industrial Research (CSIR)-Central
Food Technological Research Institute (CFTRI), Mysore, INDIA. Title of the Ph.D. thesis: “Molecular
Engineering of the horsegram (Dolichos biflorus) Seed Bowman-Birk Inhibitor: Implications of the
disulfide framework on functionality.” (Mentor: Dr. Lalitha R. Gowda).
Project Assistant at Department of Protein Chemistry and Technology, CSIR-CFTRI, Mysore.
(August 2006 to June 2009). Worked on the project entitled “Cyclic peptide as proteinase
inhibitor: purification, molecular cloning, and expression.” (Mentor: Dr. Lalitha R. Gowda).
Project work for master's programme at Department of Biophysics, All India Institute of Medical
Sciences, New Delhi, INDIA (January 2006 to April 2006). Worked on “Isolation, Purification, and
Crystallization of C-lobe of Bovine Lactoferrin.” (Mentor: Prof. T. P. Singh).
7. Publications
Journal Publications:
1. Vinod Kumar, Amit K Chaudhary, Yuxiang Dong, Haizhen A. Zhong, Goutam Mondal, Feng Lin,
Virender Kumar, and Ram I Mahato. Design, Synthesis and Biological Evaluation of novel
Hedgehog inhibitors for treating Pancreatic Cancer. Scientific Reports. 2017, 7:1665.
2. Vinod Kumar, Virender Kumar, Timothy McGuire, Donald W. Coulter, John G. Sharp and Ram I.
Mahato. Challenges and recent advances in medulloblastoma therapy. Trends in
Pharmacological Sciences. 2017, 38: 1061-1084.
3. Vinod Kumar, Saravanan Murugeson, Neha Vithani, Balaji Prakash and Lalitha R Gowda. A salt
bridge stabilized C-terminal hook is critical for the dimerization of a Bowman-Birk inhibitor.
Archives of Biochemistry and Biophysics. 566 (2015) pp 15-25.
4. Vinod Kumar, and Lalitha R. Gowda. The contribution of two disulfide bonds in the trypsin binding
domain of horsegram (Dolichos biflorus) Bowman-Birk inhibitor to thermal stability and
functionality. Archives of Biochemistry and Biophysics. 537 (2013), pp 49-61.
5. Vinod Kumar, and Lalitha R. Gowda. Production of horsegram (Dolichos biflorus) Bowman-Birk
Inhibitor by an intein mediated protein purification system. Protein Expression and Purification. 89
(2013) pp 16-24.
6. Vinod Kumar, Virender Kumar, Amit Kumar Chaudhary, Donald W. Coulter, Timothy McGuire,
and Ram I. Mahato. Impact of miRNA-mRNA Profiling and Their Correlation on Medulloblastoma
Tumorigenesis. Nat Comm (Under Review).
8. Poster/Oral Presentations
1. Design, Synthesis and Biological Evaluation of Potent Analogues of GDC-0449 for the Treatment
of Pancreatic Cancer. 4th Biopharmaceutical Research and Development Symposium at UNMC,
NE (USA). Oral presentation, 2017
2. Multi-pronged Treatment Strategies for Medulloblastoma. 15th Annual Pediatric Research Forum
at Children Hospital and Medical Center, Omaha, NE (USA). Poster, 2017
3. Synthesis and Nano-formulation of Potent Analogues of GDC-0449, a Hedgehog inhibitor for the
treatment of Pancreatic Cancer. 3rd Biopharmaceutical Research and Development Symposium
at UNMC, NE (USA). Poster, 2016
4. Association of Microbiologists of INDIA, Mysore chapter, CFTRI campus. Oral, 2014
5. Dimerization of Horsegram Bowman Birk Inhibitor is guided by an intramolecular interaction in the
monomer”. Recent Advances in computational Drug Design at IISc, India. Poster, 2013
6. Horsegram Bowman-Birk inhibitor: The contribution of interdomain disulfides to thermal and
proteolytic stability”. XXII ICFoST Meet CFTRI, India. Poster, 2012
(Note: Received second best poster presentation award)
7. Molecular engineering of Bowman-Birk inhibitor (BBI) of horsegram (Dolichos biflorus) seed: Effect
of interdomain disulfide bond deletion”. 80th Annual meeting of Society of Biological Chemists at
CIMAP, INDIA. Selected for oral presentation, 2011
8. Facile production of functional recombinant Bowman-Birk Inhibitor (BBI) using Ssp dnaB derived
self cleaving mini intein tag. Recent Trends in Biophysics at BHU, India. Poster 2010
9. Functional expression of horse gram (Dolichos biflorus) Bowman- Birk inhibitor deletion mutant
using modified self- splicing inteins. 77th Annual session & symposium of The National Academy of
Sciences (NASI), INDIA. Poster, 2007
(Note: Received best poster presentation award).
10 International symposium on Cardiovascular Diseases, Stroke and Diabetes, at AIIMS, New Delhi,
India. 2006
9. Workshops Attended
1. Bioinformatics in Genomics, Proteomics and Metabolomics at Indian Institute of Technology,
Kharagpur, India September 23-24, 2011
2. Emerging Techniques of Biophysics at Banaras Hindu University (BHU), Varanasi, INDIA.
February 13-16, 2010
10. References
Dr. Ram I. Mahato, Ph.D.
Professor and Chair,
Department of Pharmaceutical Sciences
University of Nebraska Medical Center, Omaha NE 68198 Email: [email protected]; Tel (office): (402) 559-5422
Dr. Kishore B. Challagundla, Ph.D.
Assistant Professor,
Department of Biochemistry and Molecular Biology
University of Nebraska Medical Center, Omaha NE 68198
Email: [email protected]; Tel (office): (402) 559-3441
Dr. Lalitha R. Gowda, Ph.D.
Chief Scientist (Retd),
Department of Protein Chemistry and Technology
Central Food Technological Research Institute (CFTRI), Mysore, India
E-mail: [email protected]; Tel (cell): +91-9482717138