vilber fusion imaging catalog 2013

C A P A B I L I T I E S4 I The range6 I Performance

and innovations

8 I Advanced technologies10 I Applications12 I Software

S Y S T E M S16 I Fusion Spectra18 I Fusion FX20 I Fusion SL22 I Fusion Solo

I N D E P T H26 I Fusion in details26 I DARQ camera26 I Glossary

The speed of light• Outstanding sensitivity• Lowest noise floor• Deepest cooling• Superior resolution• Largest range of application• Largest field of view• Fastest acquisition process• Best image clarity• Widest dynamic range• Best raw data protection• Quantitative stability• High Sensitivity Reading (HSR) technology• Widest build-in application library• Best band revelation• Best signal response linearity• Clarity ™ technology• Best and unrivalled V.084 optical lens• Exclusive Apps Studio library• 3D Dynamic Scan technology

The Fusion is a high-end ultra-sensitive scientific camera platform, designed to extract thelowest level of detection from your protein or DNA sample. Whether you need a dedicatedchemiluminescence system or a multimodal platform with chemiluminescence andmultispectral imaging, we can provide you the best solution.

Our superior sensitivity, resolution and dynamics provide optimal performance across a largearray of applications. There is no need to compromise. The Fusion system is ready to workfor your most demanding chemiluminescence, bioluminescence, multiplexing or fluorescenceapplications.

Our advanced set of performance is ideal to image with high fidelity and to allow quantitativescientific measurements. The Fusion systems’ cameras use the latest generation of sensorsand semi-conductors. Combined with our unique High Sensitivity Reading (HSR) technologyand our V.084 lens, the Fusion is ideal for publication, quantification and documentation gradeimaging.

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Western blot Protein gel RGB multiplexingDNA/RNA gel

Best in class

5

The Fusion Spectra is a multimodalimaging platform ideal forchemiluminescence, bioluminescence,and multispectral fluorescence.

• Chemiluminescence• Bioluminescence• Visible imaging• UV fluorescence• Multiplexing fluorescence• Choice of visible RGB, NIR

or IR excitation source

Apps Studio3D Dynamic ScanSpectral UnmixingEasyPlex

Chemiluminescence Western blot,fluorescence Western blot, plants, smallanimal, DNA gel, protein gel, Petri dish,microtitration plate imaging.

The Fusion FX is designed forchemiluminescence, UV fluorescenceand visible applications.

• Chemiluminescence• Bioluminescence• Visible imaging• UV fluorescence

Apps Studio3D Dynamic Scan

Western blot, plants, small animal, DNAgel, protein gel, Petri dish, microtitrationplate imaging.

In vivo imaging IR/NIR multiplexing IR Western blotGFP

The Fusion SL is designed forchemiluminescence application, withoptional capabilities for UVfluorescence.

• Chemiluminescence• Bioluminescence• Visible imaging• Optional UV fluorescence


Western blot, plants, small animalimaging.Optional DNA and protein gel imaging.

The Fusion Solo is a dedicatedchemiluminescence system.

• Chemiluminescence• Bioluminescence• Visible imaging

Apps Studio

Western blot, plants and small animalimaging.

Chemiluminescence blot Protein gel Luciferase on ArabidopsisWestern blot

Ultra sensitive imaging

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Fig.2: Comparative low lightimages taken with Fusion versus

competitive systems withcomparable imaging settings.

The images are displayed withsame relative intensity scaling.

FUSION

Competitor 1

Competitor 2

Competitor 3

The Fusion is an extensive portfolio of ultra-sensitive imaging platform, widely considered tobe ‘best in class’. Using the most advanced large pixel CCD technology to collect more light,in combination with our unique state of the art V.084 motorized optical lens, the Fusion FX7offers the best sensitivity and speed, and reaches the lowest limits of detection.

The Fusion’s imaging performance is automatically optimized for a wide variety of applicationrequirements:

• For chemiluminescence, the time to get the image is dramatically reduced and preciousantibody can be saved.

• For fluorescence, ultra-sensitive detection capability facilitates the use of shorter excitationexposure, thereby reducing photobleaching and phototoxicity and lowering dyeconcentrations.

Linearity

The signal response linearity describes therelationship between the detected signal andthe sample quantity within the camera dynamicrange. Linearity is critical for accuratequantification. The greater the linearity, the more preciselyproteins can be quantitated over that range.The Fusion linear range is enhanced by ourunique High Sensitivity Reading (HSR) technologywhich minimizes the noise and increases theimage dynamic. The Fusion is then extremelylinear over its wide dynamic range and caneasily detect large intensity difference betweenbright and faint bands before reachingsaturation.

Fig.1: Protein quantity versus detected signalPrecise detection and quantitation of strong as wellas weak signals using the Fusion High SensitivityReading (HSR) technology

16ng 8ng 4ng 2ng 1ng 2pg 1pg

Ethidium bromide GelGreen GelRedSYBR Safe

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FUSIONFilm

Band revelation with ClarityTM

Signal to noise

With the Fusion, dive into the layers of your samples tocapture images from the deep inside of your blot or gel. Our unique ClarityTM technology combined with our razorsharp V.084 lens enhances the revelation of your bands,even under low light condition. The image is always at itsbest, clear and sharp. The bands appear clearly and aredetached from their surroundings. The very faint bands arevisible and distinguishable.The Fusion combines the best optical lens and imagingtechnology to reach unrivalled faint bands detection.

Competitors FUSION

The aim of the Fusion is to maximize the signal and tominimize the noise to achieve the best signal to noise ratiofor the lowest limit of detection.Thanks to our High Sensitivity Reading (HSR) technology,the Fusion reduces the various sources of noise to thelowest floor and the signal can stand out from thesurrounding background. The figures 5 and 6 are acomparison of weak chemiluminescence signal imagesshowing the sensitivity benefit of the Fusion HSR technologyface to a competitive system. The HSR technology allowsto reach the lowest limit of sensitivity.

Fig.5: Competitive system. A two-fold dilution series of transferrin was

transferred to a PVDF membrane. The faint signal cannot stand out from

the surrounding noise level

Fig.6: The Fusion combined with the HighSensitivity Reading (HSR) technology provide a

better signal to noise and more bands aredetected. Fusion FX7, cooling at -42°c.

FUSIONCompetitors

Fig.3: Without ClarityTM display Fig.4: With ClarityTM display

Spectral Unmixing

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Numerous fluorescent dyes are available for multicolor fluorescence imaging. However,interpreting multicolor images can be challenging as the spectral overlap of the excitationand emission characteristics often limits the possibilities to combine these fluorescentmarkers within one sample.

The Fusion Spectral Unmixing is a powerful algorithm designed to correct the spectralcrosstalk between different color channels such as for instance, to separate the Cy2™ signalfrom the Cy3™ crosstalk. With this powerful technique it becomes possible to ascertain thecontribution of different fluorochromes to the total signal and, by chromatic redistribution ofthe intensity, to restore a clear signal for each color channel undisturbed by emission fromthe other fluorochromes. Spectral Unmixing does not create artificially embellished images.The chromatic information inherent to the data is used to redistribute the fluorescenceintensity pixel by pixel while the total pixel intensity is maintained. Thus, quantitative analysisis not only still possible but delivers more significant data.

Fig.9: Fluorescent images were acquired using a Fusion Spectra system. The sample contains Cy2™,AlexaFluor 488, Cy3™ and AlexaFluor 594. The Spectral Unmixing removed the crosstalk.

Competitor 1. Aperture f:0.95



16ng 8ng 4ng 2ng 1ng

16ng 8ng 4ng 2ng

16ng 8ng

Fig.8: At same exposure time, the V.084 lens hasunrivalled sensitivity

ECL Super Signal IR/NIR multiplexing Ethidium bromide

The V.084 lens

The Fusion custom made V.084 lens combinessensitivity and optical performance for veryfaint light conditions. The optical systemincludes ultra-low dispersion components toenhance the sensitivity and aspheric elementsto deliver consistently sharp images. The V.084lens has a focusing distance of only 25cm toprovide unrivalled sensitivity, clarity and imagequality.

Channel 1470nm excitation Overlayed

Channel 2540nm excitation

Unmixed

Raw

Cy2 AF488 Cross-talk Cross-talk Cy3 AF594

Cy3 AF594Cy2 AF488

Fusion V.084 lens. Aperture f:0.84

16ng 8ng 4ng 2ng 1ng 2pg 1pg

9

High Sensitivity Reading (HSR) technlogy

The SuperResolution with no pixel size compromise

Fusion’s High Sensitivity Reading (HSR) is a unique technologywhich delivers ultra-low noise and sensitivity without alteringthe raw image data. Thanks to a camera modular design, a dualamplifier architecture and a complete control of the electroniccomponents, the camera noise is reduced to a fraction.The HSR technology is a camera “on-head” built-in technology.During the light exposure, the non-necessary cameracomponents are shut down. Once the exposure time is over,the camera components are automatically turned back. Theshut-down process during the image exposition reduced thecamera noise, resulting in a better chip capacity to collect thesignal.

The Fusion FX7 SuperResolution technology providesunparalleled 10 megapixels resolution withoutcompromising read noise, dynamic range, field of view andsensitivity. This means up to 5 times more accurate andmore quantitative data compared to competitors.Our Fusion scientific grade CCD camera has beenspecifically designed for scientific imaging and signalmeasurement. The Fusion cameras are based on largesensor format and unrivalled pixel size combined with microlens technology. Given two sensors with equal numbers ofpixels, the sensor with the larger pixel size collects morephotons resulting in more sensitivity.

Texas Red SybrGreen SyproRubySyproRed

Fig.10: At same exposure time with aLED plate standard, the HSRtechnology provides a better

sensitivity by reducing the noiseground level. By keeping the

background to its lowest floor, thevery faint signal can easily be

detected and quantified.

Fig.11: The FusionSuperResolution

combined with ourscientific grade CCDcameras offers high

resolution withoutpixelisation and unrivalled

sensitivity.

Competitor 1 -10°c

Competitor 2 -20°c

Competitor 3 -30°c

FUSIONHSR technology

Competitor 1,4 megapixels

Competitors’ cameraschip size

FUSION10 megapixels

FUSION cameras chip size

Competitor2/3 of inch

Competitor1/2 inch

FUSION FX71,2 inch

FUSION FX51 inch

The Apps Studio

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The Fusion Apps Studio is a revolutionary approach to imaging. The Studio is an innovativelibrary of applications which contains more than 40 different protocols for your blot, gel andother bioluminescence samples. The protocols are easily accessible and gathered into fourmaster protocols: DNA/RNA gels, Protein gels, Protein blots, Bioluminescence and others.

The Studio contains the excitation and the emission spectra of the main fluorophores usedin modern molecular biology laboratory. It also suggests the best possible systemconfiguration in terms of excitation light source, emission filter and sensitivity level. The AppsStudio ensures reproducibility and one click image acquisition for the best ease of use.

Fig.12: Apps Studio contains more than 40 different protocols for your blot, gel and otherbioluminescence sample.

Multimodal platform

Western blot Western blot Microtitration plateDNA/RNA gel

Chemiluminescence

Bioluminescence

Fluorescence

Biofluorescence

Multiplexing fluorescence

The Fusion is the first multimodal imagingplatform designed for molecular biologylaboratories. It can cover chemiluminescence,bioluminescence, fluorescence, multiplexingfluorescence, multispectral imaging andchemifluorescence applications.

The Fusion can accommodate up to 7 excitations channels in the IR, NIR, visible andUV area and is ideal for a large array ofapplications such as Western blot chemi-luminescence, Western blot fluorescence, 1D DNA gel, 1D protein colorimetric samples,stain free gel and blot…

The modularity design of the Fusion makes thesystem fully upgradable. You can simply startwith a dedicated chemiluminescence systemand upgrade your system as your number ofapplications grows.

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Chemiluminescence and bioluminescence

Fluorescence and multi-fluorescence

Chemiluminescence is the main method used to detectproteins in Western blotting. The chemiluminescentreaction occurs when an enzyme such as horseradishperoxidase (HRP) or alkaline phosphatase reacts with achemiluminescent substrate (such as luminol or dioxetane)to produce a weak signal. The emitted light has awavelength between 400 and 500 nm.The Fusion system is the chemiluminescence imagingmaster thanks to its unrivalled sensitivity and lowest noise.This makes the system ideal not only for Western blot, butalso for luciferase on plants or small animals, and any otherbioluminescence sample.

Fig.13: Luciferase lightencapsulatedin a PCR tube

Fluorescence is the main method used for gene expressionand protein detection. It results from a process that occurs inmolecules known as fluorophores. The fluorophore absorbsthe excitation light, reaching a higher energy state. Byreturning to its former state, it emits fluorescent light. The aimof the imaging system is to separate the emitted light from theexcitation light in order to obtain an optimum sample image.The Fusion can accommodate up to 7 excitation and emissionchannels in the IR, NIR, visible and UV area and is ideal for alarge array of applications such as Western blotfluorescence, 1D DNA gel, 1D protein colorimetric samples,multiplexing, stain free gel and blot…

Fig.14: The Fusion can accommodate up to 7 excitation and emission channels in

the IR, NIR, visible and UV area

Bacteria imaging Small animal imaging 2D gelDot blot

Precise and intuitive Fusion 3D ScanFusion has an outstanding ease of use.The system and software are ergonomicallydesigned. The simple and self-understandableinterface is very user friendly.

Combined with the Apps Studio, the protocoldriven image acquisition is as quick as intuitive:adjust your exposure, save, print or quantify.

Thanks to the auto-exposure mode, the systemwill automatically capture the best image withjust one click. There is no need for exposuretime guessing and image stacking. The Fusionautoexposure mode automatically provides youthe best possible image from your sample.

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A New Direction for Imaging Technology

The Fusion 3D scan is a revolutionary approach in the field of molecular imaging. The Fusion3D Scan images your sample in real time reconstructs the data to create live threedimensional model. The 3D reconstruction provides direct information regarding the imagedynamic, background level and protein or DNA quantity. A little change of exposure time willrefresh the 3D view automatically. The saturation effect can be controlled live before theimage is freezed.

The Fusion 3D Scan opens a new frontier for protein or DNA imaging. 3D modeling will changethe way you see your 1D blot or gel image into 3D results.

Fig.15: The Fusion interface is well organized andintuitive Fig.16: The 3D scan is performed while the image is acquired by the camera opening new area for

molecular biology

Western blot DNA/RNA gel PCR control Macro array

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Image Master Assistant

Our exclusive Image Master helps you to obtain the optimum image at aglance. It automatically monitors the maximum and the minimum intensityobtained on the image, indicates it’s dynamic, signal to noise ratio, and warnsyou about pixel saturation.The Image Master informs you when the raw data are affected by a post-processing function such as background subtraction or image addition.The Image Master is simply perfect for quantification and publication as ithelps you to keep the control on the image, making sure your image is alwaysappropriate whatever its use.

Superior quantitative results

Protein gel Protein gel IR/NIR Western blot GFP

The Fusion system is directly integrated with the Bio-1D software forquantification and normalization. Bio-1D is a sophisticated and intuitiveanalysis software designed for virtually any fluorescence orchemiluminescence sample. The software combines the power of acomprehensive set of analytical tools and automatic functions in anincredible ease of use environment. It includes 8 different analysis modulesfrom molecular weight calculation to volume quantification, throughmicrotitration, colony counting, distance calculation and proteinquantification.The analysis can be saved as a template and re-used for further analysis tofacilitate routine analysis. All result tables and graphics can be printed orexported in a Windows® compatible format.

Fig.18: The ImageMaster Assistant isa powerfull tool to

control your sampleimage quality. A

modification of theimage display does

not modify theImage Master data.

Fig.17:The Bio-1D

Quantificationsoftware

is smoothlyintegrated

in the Fusioninterface

C A P A B I L I T I E S4 I The range6 I Performance

and innovations

8 I Advanced technologies10 I Applications12 I Software

S Y S T E M SI Fusion SpectraI Fusion FXI Fusion SLI Fusion Solo

I N D E P T H26 I Fusion in details26 I DARQ camera26 I Glossary

Spectra inside Multimodal imaging platform The Spectra LED light module is designed to add a wide range of applications to your system. With Fusion Spectra, you have 7 excitation wavelengths at your fingertips,ranging from UV to infrared. Up to 4 NET-LEDmodules can be installed simultaneously in thesystem in addition to several UV excitationsources.

The very tight LED spectrum is additionallyconstrained with a very narrow excitation filter.

This means less background in the images ofyour sample and a higher signal to noise ratioto detect the weakest signals.

The FUSION FX Spectra is the first multimodal imaging platform designed for molecular biologylaboratories. It covers chemiluminescence, bioluminescene, fluorescence, multiplexingfluorescence and chemifluorescence applications. Thanks to the Spectra LED modules, thesystem can accommodate up to 7 excitation channels in the IR, NIR, visible RGB and UV area.

The FUSION FX Spectra is ideal for a large array of applications such as Western blotchemiluminescence, Western blot fluorescence, 1D DNA gel, 1D protein colorimetric samples,stain free gel and blot… You can for instance detect two proteins on the same blot usingfluorescence labeling, protein expression can be quantitated relative to a housekeepingprotein without the need for stripping and reprobing. Protein signals can be overlayed so thatboth signals can be visualized simultaneously. Triplexed detection can also be performed forinstance for the detection of two proteins of interest, for example, a protein with and onewithout post translational modification (PTM), normalized to a housekeeping protein on thesame blot.

Each individual light source delivers only a precisely defined range of the spectrum. As aresult, you benefit from extremely high-contrast images – images that will enable you toachieve meaningful scientific results in tasks ranging from simple routine applications tocomplex multiplex applications. The spectral overlap is minimized with the 100nm separationgap of the excitation sources and narrow optical filtering of the emission source. The LEDSpectra modules can be easily changed, meaning that Fusion can be adapted simply as therequirements of your applications evolve.

Fig.19: The large Spectra LED Array (right) provides auniform illumination over a large surface compared tocompetitive spot of light solution (left)

Fig.20: Fusion Spectra excitation and emission sources in the UV, Visible, NIR and IR ranges.

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Western blot Luciferase assay IR imaging DNA/RNA gel

Competitors FUSION

Key features Configurations

7 excitations channels options in the IR, NIR,visible RGB and UV area10 Spectra narrow band filters available from440 to 850nm40 fluorescence and chemiluminescencepredefined imaging protocols at your fingertipsSame or better sensitivity than a filmUnique V.084 lens performanceSuper speed image acquisition3D Dynamic Scan TechnologyNetLed Spectra technology for a large andoptimum uniform illumination areaLong life lasting LED for thousands of hours of useIntuitive Apps Studio interface Spectral Unmixing for signal cross talk separationInstant cooling for fast availability of the systemHigh Sensitivity Reading™ Technology for abetter signal to noise ratioCFR21 Part 11 readyFull GLP complianceClarity™ technology for razor sharp bandrevelation

SuperResolution™ technology for ultimatemegapixel resolution

Large sensor DARQ scientific CCD camera

Large pixel size

Pure image integrity and raw data protection

ImageMaster™ technology to obtain theoptimum image at a glance

Free software for image acquisition and imageanalysis

Fast and easy quantification using housekeepingprotein

EasyPlex™ technology for fast and easy imageacquisition of several dyes on the same sample

Stainless steel, aluminium and steel darkroom forthe best robustness

Reproducible and comparable quantification data

Scientific TIFF file or proprietary file format

UV, SuperBright or SkyLight transilluminatorincluded

Interchangeable transilluminator

Choice of filtered EPI UV 254nm, 365nm and blue470nm

Spectra rangeChoice between three different configurations:

• Spectra IR/NIR/G: three channels, green, N IR, IR• Spectra RGB: three channels, blue, green and red • Spectra One: one channel either blue, green, NIR or IR

Spectra emission filters not included. Optional EPI blueTransilluminator and F590 included for standard gel applications

FUSION FX Spectra series

Fully automatic. Motorized filter wheel. Motorized V.084 lens• FUSION FX7 Spectra with the DARQ7 camera• FUSION FX5 Spectra with the DARQ5 camera

Fusion SL Spectra series

Semi-automatic. Motorized filter wheel. Manual V.084 lens• FUSION SL4 Spectra with the DARQ7 camera• FUSION SL2 Spectra with the DARQ5 camera

FUSION-Capt Spectra software

Apps Studio - 3D Dynamic Scan - Spectral Unmixing - EasyPlex

Options

Choice of 10 different Spectra emission filtersSkyLight transilluminator or Super-Bright MX transilluminatorWhite light or blue light conversion screenEPI UV 254nm, 365nm and blue 470nm

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RGB multiplexing NIR imaging In vivo imagingProtein gel & blot

Spectra ready A complete integrated system for Western blot and gel imagingThe Fusion FX can image both chemi-

luminescence and fluorescence applications.For fluorescence, the Fusion FX system is easilyupgradeable to the LED Spectra module forblue, green, near infrared or infraredillumination.

The Spectra module is ideal for a large array ofapplications such as Western blot chemi-luminescence, Western blot fluorescence, 1D DNA gel, 1D protein colorimetric samples,stain free gel and blot…

The unrivalled Fusion FX is ideal for chemiluminescence, bioluminescence and UVfluorescence applications. With the FX system, you can see your blot in the new light ofultrasensitive imaging at speeds never achieved before.

The FX is based on an advanced set of performance ideal to high fidelity and quantitativescientific measurement. The camera modular electronic design and the dual amplifierarchitecture deliver raw data with virtually no loss of information, no image distortion, noimage processing, no pixelization, and with ultimate sensitivity and dynamic range.

The massive metal made Fusion FX darkroom has been designed for long durability and lighttightness. With the aid of its integrated processor technology, the intelligent darkroomsupervises monitors and controls all system functions. As an example, sensors identify thedistance of the object, the intensity of the LED illumination is optimally chosen, and automaticfocus can be used.

18

SuperSignal ECL SybrSafe Ethidium bromide

19

GelRed SybrGreen FITCGFP

Features & benefits Configurations

Numerous fluorescence and chemiluminescencepredefined imaging protocols at your fingertipsSame or better sensitivity than a filmUnique V.084 lens performanceSuper speed image acquisition3D Dynamic Scan TechnologyLong life lasting white light LED for thousands ofhours of useIntuitive Apps Studio interface Instant cooling for fast availability of the systemHigh Sensitivity Reading™ Technology for abetter signal to noise ratioCFR21 Part 11 readyFull GLP complianceClarity™ technology for razor sharp bandrevelationSuperResolution™ technology for ultimatemegapixel resolutionLarge sensor DARQ scientific CCD cameraLarge pixel sizePure image integrity and raw data protectionImageMaster™ technology to obtain the

optimum image at a glance



Intensity of the white light LED illuminationautomatically adjusted


Smart sensors to identify the distance of theobject


Autofocus

Autoexposure

Motorized filter wheel


UV, SuperBright or SkyLight transilluminatorincluded

Interchangeable transilluminator


FUSION FX seriesFully automatic systems with a motorized filter wheel and amotorized V.084 lens

• FUSION FX7 Spectra with the CX7 camera• FUSION FX5 Spectra with the CX5 camera

Transilluminator and F590 included for standard gel applications

FUSION Capt FX software


Options

SkyLight transilluminatorSuper-Bright MX transilluminatorWhite light or blue light conversion screenEPI UV 254nm, 365nm and blue 470nmSpectra modules and Spectra emission filters

Performance for your blot Modular and upgradeable chemiluminescence systemThe FUSION SL is the ideal system for

laboratories that need to find an alternative totheir film for Western blot imaging usingchemiluminescence protocol. It can also beused for any applications using luminescentsubstrate such as luciferase, luminol or ECLequivalent.The system does not compromise in terms ofimaging performance. The SL DARQ7 cameraoffers the deepest Peltier cooling availablefrom any Western blot imaging system on themarket, critical for minimization of both noiselevel and sensitivity.In standard, the Fusion SL is equipped with theV.084 lens. The V.084 has unrivalled f/0.84sensitivity and allows the sample to be at 25 cmfrom the camera.

The Fusion SL system is a modular system with extendable capabilities. The built-in capabilityof the system is preliminary chemiluminescence and bioluminescene applications. You canthen upgrade the system if you need UV fluorescence for your gel or Epi-light module for thefluorescence of your blot. The system can grow according to your laboratory need.

The system is also equipped with a 6 positions motorized filter wheel and comes with the AppsStudio and the 3D scan features. Thanks to its modular concept, different options can be addedto the system:

- UV Transilluminator - White light plate- LED Spectra module - Blue light conversion screen- Fluorescent emission filter

The DARQ7 camera offers the deepest Peltier cooling available from any Western blot imagingsystem on the market, critical for minimization of both noise level and sensitivity. Deep coolingmeans the low noise advantage can be maintained under all exposure conditions, thus forboth chemiluminescence and fluorescence applications.

The massive metal made Fusion SL darkroom has been designed for long durability and lighttightness. With the aid of its integrated processor technology, the intelligent darkroomsupervises monitors and controls all system functions. As an example, sensors identify thedistance of the object, the intensity of the LED illumination is optimally chosen, and the filterposition is automatically selected according to your application.

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Western blot DNA/RNA gel Luciferase assay Southern blot

21

Western blot 1D gel Protein gel Chemiluminescence blotting


Numerous chemiluminescence predefinedimaging protocols at your fingertipsSame or better sensitivity than a filmUnique V.084 lens performanceSuper speed image acquisition3D Dynamic Scan TechnologyLong life lasting white light LED for thousands ofhours of useIntuitive Apps Studio interface Instant cooling for fast availability of the systemHigh Sensitivity Reading™ Technology for abetter signal to noise ratioCFR21 Part 11 readyFull GLP complianceClarity™ technology for razor sharp bandrevelationSuperResolution™ technology for ultimatemegapixel resolutionLarge sensor DARQ scientific CCD cameraLarge pixel sizePure image integrity and raw data protectionImageMaster™ technology to obtain the

optimum image at a glance



Intensity of the white light LED illuminationautomatically adjusted


Smart sensors to identify the distance of theobject


Autofocus

Autoexposure

Motorized filter wheel


Optional UV, SuperBright or SkyLighttransilluminator


Fusion SL seriesSemi-automatic systems with a motorized filter wheel and amanual V.084 lens

• FUSION SL4 with the DARQ7 camera• FUSION SL2 with the DARQ5 camera

Transilluminator and F590 for standard gel applications can beoptionally added to the SL system

FUSION-Capt SL software


Options

Standard UV transilluminatorSuper-Bright MX UV transilluminatorSkyLight transilluminatorWhite light or blue light conversion screenEPI UV 254nm, 365nm and blue 470nm

Maxi signal Dedicated chemiluminescence imaging system

4 tray positionsThe Fusion Solo has a removable tray and 4 positioningoptions according to the sample size. The sample canbe positioned as close as possible from the camera inorder to maximize the sensitivity and several samplescan be imaged at the same time.

The Fusion Solo is the ideal system for laboratories that need to find an alternative to their film for Western blot imaging using chemiluminescence protocol. It can also be used for any applications using luminescent substrate such as luciferase, luminol or ECL equivalent.

The blot visible marker can be easily imaged thanks to the white light LED and merged to theblot signal. Thanks to our exclusive paste marker function, the merged image can be quantifiedwith no loss of the raw data.

The Fusion Solo 4 has the largest field of view available on the market for a product of itsrange and provides images for accurate quantification and publication.

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Southern blot Plant imaging Small animal imaging Western blot

f/0.84 lensAs standard, the Fusion Solo is equippedwith the V.084 lens. The V.084 has unrivalledf/0.84 sensitivity and allows the sample tobe at 25cm from the camera.

Same or better sensitivity than a film

The Fusion Solo is the ideal system for usersthat need to find an alternative to their filmwhich are more and more discontinued insidelaboratories. The Solo is ideal for accuratequantification and publication grade images.

Unrivalled ease of use

The Fusion Solo software is the easiestsoftware to take an image. Place your blot onthe tray; click on “auto exposure” and thesystem will deliver an image ready to bequantified.

Razor sharp image

Thanks to its unique lens and cameratechnologies, the Fusion Solo delivers raw datawith no loss of information, no image distortion,no image processing, no pixelization, and nobackground noise.

Bench-top design

Compact and elegant, the Fusion Solo wouldperfectly fit in any laboratory looking for spacesaving.

F/0.84 lens

The V.084 has unrivalled f/0.84 sensitivity andallows the sample to be at 25cm from thecamera.

The largest field of view

The Fusion Solo 4 has the largest field of viewavailable on the market for a product of its range.

Robust hardware

Steel, stainless steel, aluminium are the maincomponents of the Fusion Solo. The system isdesigned to last.

-67°C cooling

The Fusion Solo 4 camera is cooled to minus67°C. The images have naturally nobackground. There is no need to post processthe images with the risk of affecting the rawdata and of losing some bands.

White light for marker imaging

The blot visible marker can be easily imagedthanks to the white light LED.Printer sizeThe Fusion Solo is a bench top instrument. Itcould easily find its place in a busy laboratory.

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Blot normalisation Dot blot MacroarrayQuantitative Western blotting


Fusion Solo seriesDedicated systems with a manual V.084 lens

• FUSION Solo 4 with the CX7 camera• FUSION Solo 2 with the CX5 camera

FUSION-Capt SL software

Apps Studio

26

Intelligent Darkroom conceptFully-automatic system• Motorized optical lens• Motorized filter wheel• Software control of the lighting• Automatic recognition of the sample

position• Automatic visible lighting adjustment• Autofocus• Auto-exposure

Choice between the DARQ7 or theDARQ5 cameraMotorized V.084 lens

Fusion-Capt software for imageacquisition and image analysis• Apps Studio• 3D Dynamic Scan

Intelligent Darkroom conceptFully-automatic system• Motorized optical lens• Motorized filter wheel• Software control of the lighting• Automatic recognition of the sample

position• Automatic visible lighting adjustment• Autofocus• Auto-exposure

Choice between the DARQ7 or theDARQ5 cameraMotorized V.084 lens


Intelligent Darkroom conceptSemi-automatic system• Motorized filter wheel• Software control of the lighting • Automatic recognition of the sample

position• Automatic visible lighting adjustment• Auto-exposure

Choice between the DARQ7 or theDARQ5 cameraManual V.084 lens Fusion SL2 Xpress:Possibility to equip the DARQ5 camerawith a motorized zoom lens withaperture f:1.2 and optical zoom 6 times


Manual control• Auto-exposure

Choice between the DARQ7 or theDARQ5 cameraManual V.084 lens

Fusion-Capt software for imageacquisition and image analysis• Apps Studio

IR imaging GelGreen Macro array Ethidium bromide

27

Glossary

Background level: Some degree of noise is always presentin any electronic CCD cameras. Eventhough noise is unavoidable, it canbecome so small relative to the signalthat it appears to be nonexistent. Thedanger is to have the background levelhigher than the signal. In that case, theprotein signal cannot be seen.

CCD technology: CCD cameras are made of sensorstransfer every collected photon into anelectron, namely into digital information.The photons are transferred into pixels.Those pixels will enable thequantification of data.

Excitation filter: Our Spectra LED module (RGB and IR) ismade of a narrow excitation filter. Whenyou excite your sample stained with aspecific fluorophore it is important to usea narrow band pass in order to get asclose as possible to the excitation peakrequired by the fluorophore.

High Sensitivity HSR is a proprietary technology which Reading (HSR) reduces the various source of noise to technology: the lowest floor level so that the lowest

signals can stand out from thesurrounding background.

Multiplexing The multiplexing is the multiple analysis(or multispectral of different type of protein on a single blot fluorescence): or gel. Each protein is stained by one

specific fluorophore that will react withone specific excitation source. The aim isto localize and see the interactionbetween different proteins.

Quadruple Peltier A thermoelectric Peltier transforms an stages: electrical current into a temperature

difference. Four stages enable to achievea fast, deep and uniform cooling.

Spectral overlap The spectral overlap is an undesirable(or cross-talk): effect of excitation of 2 different dyes

sharing the same excitation range. Theoverlap could be compensated using theFusion spectral unmixing algorithm.

DARQ cameras

DARQ7Scientific grade CCD cameraGrade 0, zero defectImage resolution: 10 megapixels Native resolution: 2048x2048

1.3 inch sensor7.4 μm square pixels

-67° C maximum cooling differentialfrom ambient-42°C absolute and regulated cooling via four stage Peltier thermoelectriccooler.

High Sensitivity reading (HSR)technologyUSB connection

DARQ5Scientific grade CCD cameraGrade 0, zero defectImage resolution:7.6 megapixelsNative resolution: 1600x1200

1 inch sensor7.4 μm square pixels

-55° C maximum cooling differentialfrom ambient-30°C absolute and regulated cooling via three stage Peltier thermoelectriccooler.

High Sensitivity reading (HSR)technologyFireWire connection

Western blot Small animal imaging Texas RedLuciferase on Arabidopsis

TURN YOUR SEARCH INTO ANSWERSVILBER is the leading European providerof molecular imaging systems, analysis softwareand UV fluorescence equipment. Founded over 50 years ago to serve the research,VILBER has pioneered the post electrophoresis marketand introduced breakthrough products such asstand alone gel-documentation, Bio-1D imaging software, Super-Bright UV technology,dedicated chemiluminescence imaging system and 3D approach to 1D gel analysis.Through a network of owned subsidiary officesand local distributors located in over 60 countries around the world, VILBER offers a broad range of products.For more information about VILBER, visit our website at www.vilber.com

HEADQUARTERVilber LourmatBP-66 – ZI Sud TorcyF-77202 Marne-la-Vallée cedex 1FranceT.: 33 (0) 1 60 06 07 71F.:33 (0) 1 64 80 48 [email protected]

GERMANY OFFICEVilber Lourmat Deutschland GmbHWielandstrasse 2D-88436 EberhardzellDeutschlandT.: 49 (0) 7355 931 380F.: 49 (0) 7355 931 [email protected]

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vilber fusion imaging catalog 2013

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