vce vet laboratory skills · 2016 vet lab skills exam 4 question 11 sterilised saline solutions are...

VCE VET LABORATORY SKILLSWritten examination

Wednesday 16 November 2016 Reading time: 9.00 am to 9.15 am (15 minutes) Writing time: 9.15 am to 10.45 am (1 hour 30 minutes)

QUESTION AND ANSWER BOOK

Structure of bookSection Number of

questionsNumber of questions

to be answeredNumber of

marks

A 20 20 20B 17 17 80

Total 100

• Studentsarepermittedtobringintotheexaminationroom:pens,pencils,highlighters,erasers,sharpeners,rulersandonescientificcalculator.

• StudentsareNOTpermittedtobringintotheexaminationroom:blanksheetsofpaperand/orcorrectionfluid/tape.

Materials supplied• Questionandanswerbookof19pages.• Answersheetformultiple-choicequestions.

Instructions• Writeyourstudent numberinthespaceprovidedaboveonthispage.• Checkthatyournameandstudent numberasprintedonyouranswersheetformultiple-choice

questionsarecorrect,andsignyournameinthespaceprovidedtoverifythis.• AllwrittenresponsesmustbeinEnglish.

At the end of the examination• Placetheanswersheetformultiple-choicequestionsinsidethefrontcoverofthisbook.

Students are NOT permitted to bring mobile phones and/or any other unauthorised electronic devices into the examination room.

©VICTORIANCURRICULUMANDASSESSMENTAUTHORITY2016

SUPERVISOR TO ATTACH PROCESSING LABEL HEREVictorian Certificate of Education 2016

STUDENT NUMBER

Letter

2016VETLABSKILLSEXAM 2

Question 1Whilealaboratorytechnicianischeckingsomemicroscopes,shenoticesthetagsontheelectricalleadsareoutofdate.Whatshouldshedo?A. Informthelaboratorysupervisor.B. Replacethemicroscopeswithnewones.C. Testtheelectricalleadsherselfwithamultimeter.D. Cleantheelectricalleadswithethanoltoremoveanyoil.

Question 2Whichoneofthefollowingpiecesofequipmentinalaboratorydoesnotregularlyrequireacalibrationoftemperaturecheck?A. dryingovenB. microwaveovenC. refrigerationunitsD. autoclaveforsterilising

Question 3Whenperforminganasepticsamplingtechniqueonadairyfoodproductionline,asidefromalaboratorycoat,whatwouldbethemostappropriateprotectiveclothingtouse?A. acapforthehair,closedfootwearandsafetyglassesB. acapforthehair,gloves,closedfootwearandsafetyglassesC. latexgloves,afacemask,acapforthehairandsafetyglassesD. afacemask,acapforthehair,closedfootwear,glovesandsafetyglasses

Question 4Toprepareasolutionofapproximately0.1Msodiumhydroxide(NaOH),therelevantstandardmethodistoweigh2.00gofNaOHpellets,thenA. placea500mLstandardflaskinanicebath,add200mLofdistilledwaterandtheNaOHpellets,

agitatetodissolve,andmakeuptothemarkwithdistilledwater.B. add200mLofdistilledwatertoa500mLstandardflask,addtheNaOHpellets,dissolveandthenmake

uptothemarkwithdistilledwater.C. placea500mLstandardflaskinanicebath,addtheNaOHpelletstotheflask,thenadddistilledwater

andmakeuptothemark.D. placetheNaOHpelletsina500mLstandardflask,dissolveindistilledwaterandthenmakeuptothe

mark.

SECTION A – Multiple-choice questions

Instructions for Section AAnswerall questionsinpencilontheanswersheetprovidedformultiple-choicequestions.Choosetheresponsethatiscorrect forthequestion.Acorrectanswerscores1;anincorrectanswerscores0.Markswillnotbedeductedforincorrectanswers.Nomarkswillbegivenifmorethanoneansweriscompletedforanyquestion.

SECTION A – continued

3 2016VETLABSKILLSEXAM

Question 5ThebestwaytocleananddisinfectaninoculatingloopbeforereturningittotheequipmentstoreistoA. holdtheloopintheblueflameofaBunsenburneruntilitturnsorange.B. holdtheloopintheyellowpartofaBunsenburnerflameforthreeseconds.C. soaktheloopindetergentandwaterbeforeallowingittodrynaturally.D. washtheloopindetergentandwater,anddryitwithapapertowel.

Question 6WhatdoesSOPstandfor?A. safeoperatingprotocolB. safeoperatingprocedureC. standardoperatingprotocolD. standardoperatingprocedures

Question 7Whenperforminganasepticsamplingtechniqueonafoodsample,themostappropriateequipmenttousewouldbealaminarflowcabinetaswellasA. aBunsenburner,aninoculantloop,sterileglasswareandagrowingmedium.B. ethanol,aninoculantloop,sterilecleanglasswareandagrowingmedium.C. aBunsenburner,aninoculantloop,glasswareandagrowingmedium.D. aBunsenburner,aninoculantloopandsterilecleanglassware.

Question 8ItisnecessarytokeeptheworkingareanearamicroscopecleaninordertoA. createapleasantworkenvironment.B. preventcontaminationbyoilordust.C. stopamessbeingmade.D. helporganisethearea.

Question 9Whichofthefollowingorganellesareincludedinabasicanimalcell?A. cellmembrane,chloroplastandmitochondriaB. cellmembrane,nucleusandmitochondriaC. largevacuole,nucleusandmitochondriaD. cellwall,nucleusandmitochondria

Question 10Whichofthefollowingwouldbeleast likelytobeincludedinanemployee’scompanyhandbookforservicingcustomerrequirements?A. makingtestresultsreadilyavailablewhenrequested,beforebeingapprovedbyasupervisorB. followingenterprisepolicyandproceduresC. workingdiligentlyandresponsiblyD. behavinghonestlyandopenly

SECTION A – continuedTURN OVER


Question 11Sterilisedsalinesolutionsareusedasanemergencyeyewashinalaboratory.Asterilisedsalinesolutionismadebyadding9.5gofsodiumchloride,NaCl(s),towaterandthenmakingthevolumeupto100mLwithwater.Inadditiontothechemicalname‘sodiumchloride,NaCl(aq)’,whichoneofthefollowingconcentrationswillthelabeldisplay?A. 0.95%m/vB. 9.5%v/vC. 9.5%m/vD. 95%m/v

Question 12Whatisthepurposeofswabbingaworksurfaceimmediatelyaftercleaningwithethanol?A. tomaintainsterilityB. topreventcross-infectionC. topreventcross-contaminationD. tocheckformicrobiologicalgrowth

Question 13Whatisthemostappropriatewaytocleanupamicroscopeimmersionoilspillonalaboratorybench?A. Wetdownwithwaterandwipeupthespillwithapapertowel.B. Putanacidsolutionontheoilandwipeupthespillwithapapertowel.C. Putastrongbasesolutionontheoilandwipeupthespillwithapapertowel.D. Wetdownwithadetergentsolutionandthenwipeupthespillwithapapertowel.

Question 14Pyrexglasswarewouldnotbesuitableforwhichoneofthefollowingprocedures?A. forstoringculturemediainacupboardB. whenheatingsolutionsaboveaBunsenburnerC. forstoringstrongcausticsolutionsinacupboardD. whenheatingsolutionsbyplacingthemstraightontoahotplate

Question 15SustainableenergyworkpracticeswouldnotincludeA. examiningworkpracticesthatuseexcessiveelectricity.B. liftingmanuallyinsteadofusingmachines.C. switchingoffequipmentwhennotinuse.D. minimisingprocesswaste.

Question 16Thesafestandmostappropriatemethodofhandlingcoolglassware,includingflasks,bottlesandbeakers,istoholditA. bythesidesonly.B. bythesidesandthebottomonlyandnotbythetoportherim.C. atthetoparoundtherim,whileusingtheotherhandtosupportthebottom.D. atthetoparoundtherimofabeakerandaroundtheneckofabottleandflask.

SECTION A – continued


Question 17Alaboratorytechnicianhasthetaskofcleaningoutthechemicalstoreroom.Duringthistask,hefindsasmall,sealedbottle.Thelabelonthebottleishardtoreadandthetechniciancanunderstandonlyasmallpartofit.Whatshouldhedo?A. Placethebottleinthechemicalrubbishbin.B. Tipthecontentsdownthesinksothebottlecanbere-used.C. Informthelaboratorysupervisorandplacethebottleinadesignatedareaforsafedisposallater.D. Obtainanewlabel,includetheinformationthatcanbeunderstoodandmakealogicalguessaboutthe

restoftheinformation.

Question 18Ethanolsolutionisveryusefulinthelaboratoryasasolventandforcleaningsurfaces.Asolutionofethanolhasaconcentrationof10%v/v,butaworkingsolutionof2%v/visrequired.Howmuchwaterneedstobeaddedto50mLofthe10%v/vtomaketheconcentration2%v/v?A. 5mLB. 50mLC. 200mLD. 250mL

Question 19Aclientcallsandasksatechniciantochangesometestresultsastheclientbelievesthemtobeincorrect.Oncheckingthetestresults,thetechniciandiscoversatransfererror.Whatactionshouldthetechniciantake?A. Changethetestresultsandreissuethereportaftercheckingwiththelaboratorymanager.B. Changethetestresultsandreissuethereportbeforecheckingwiththelaboratorymanager.C. Changethetestresultsandreissuethereportaftercheckingwithacolleague.D. Changethetestresults,reissuethereportandapologisetotheclient.

Question 20Whenusingglassware,whichoneofthefollowingfactorsaffectstheaccuracyoftheresults?A. achipontherimofabeakerB. ahairlinecrackonthesideofabeakerC. atextamarkontheoutsideofabeakerD. alabelplacedontheoutsideofabeaker

END OF SECTION ATURN OVER


Question 1 (6marks)Identifythehazardwarningindicatedforeachofthesafetysignsbelow.Listtheassociatedpersonalprotectiveequipment(PPE)thatwouldberequiredwhenworkingwithsolutionsandmaterialsinvolvingeachhazardinalaboratorysetting.

1.

Hazardwarning

PPErequired

2.

Hazardwarning

PPErequired

3.

Hazardwarning

PPErequired

SECTION B – Short-answer questions

Instructions for Section BAnswerall questionsinthespacesprovided.

SECTION B – continued


Question 2 (3marks)Listthreegoodpracticesthatsupportasustainableenergyapproachintheworkplace.

1.

2.

3.

SECTION B – continuedTURN OVER


Question 3 (7marks)AtechnicianworkinginapathologylaboratoryhasbeenaskedtocarryoutaGramstaintestonasamplesuspectedtobeSalmonella enterica.Theresultsseenontheslideareshownbelow.Theresultsappearpinkincolour.

a. Describethetestresultsthatwouldberecordedforthisslide. 1mark

b. Givetwo reasonsthatexplainthetestresultsdescribedinpart a. 2marks

c. TherearetwocoloursthatindicatetheresultsofaGramstaintest.Oneoftheresultsisindicatedbythecolourpink.

Identifythecolouroftheotherresult. 1mark

d. Explainhowthetestcausesthedifferencesincolour. 3marks



Question 4 (6marks)a. Explainthedifferencebetweencross-infectionandcross-contamination. 2marks

b. Severalsampleswereacceptedbyalaboratoryformicrobiologicaltesting.

Givetwotechniquesthatshouldbeusedtotransferthesamplesasepticallyinordertopreventcross-contamination.Provideanexampleforeachtechnique. 4marks

Technique1

Example

Technique2

Example

Question 5 (3marks)a. Givetworeasonsforalaboratorytechniciantohaveanunderstandingofthebusinessgoals,

productsandservicesofthecompanytheyworkfor. 2marks

1.

2.

b. Giveoneexampleofhowalaboratorytechnician’ssuggestionstoimproveproductsandservicescouldassistinsupportingthecompany’sgoals. 1mark



Question 6 (5marks)Alaboratorytechnicianhasbeenaskedtoexamineaslideunderamicroscope.Animageoftheslideisshownbelow.Theslideshowsasampleofanimalcells.

a. Twoofthesecellslookverydifferentfromtheotherfive.

Giveanexplanationofwhatmightbehappeningtothesetwocells. 2marks

b. Whatisthebiologicaltermforthisprocess? 1mark

c. Hasthisslidebeencontaminated?Giveonereasonforyouranswer. 2marks



Question 7 (2marks)Thediagrambelowshowsaclose-upofasectionofthesideofa100mLmeasuringcylinder.Thescaleshownonthesideofthemeasuringcylinderislabelledinincrementsof10mL,withsmallerincrementsinbetween.

60

50

40

a. Atechnicianusesameasuringcylindertomeasure50mLofasolution.

Onthemeasuringcylinderabove,indicatethepositionofthemeniscusthatwouldgivethemostaccuratereadingfor50mLofasolution. 1mark

b. Anothertechnicianinthesamelaboratoryhasassistedinmeasuring50mLofthesamesolutionineachoffourothermeasuringcylinders.Thesehavebeenleftonthebench,readytobeusedinalaboratoryprocedure.

Thetechnicianchecksthesemeasuresbeforecontinuingwiththeprocedureandfindsthatoneofthecylindershas52mLofthesolution.

Whatshouldthetechniciandonext? 1mark



Question 8 (6marks)Atypicallightmicroscopeisshownbelow.

a. Labelthefollowingpartsofthelightmicroscopeonthediagramabove:• stage• eyepiecelens• objectivelens• condenser 4marks

b. Whatisthepurposeofeachofthefollowingpartsofthelightmicroscope? 2marks

• Objectivelens

• Condenser



Question 9 (2marks)Describehowtheenvironmentalimpactofwastegeneratedinalaboratoryorproductionlinecouldbeminimisedintheworkplace.Givetwoexamplestosupportyouranswer.

Question 10 (5marks)Thefollowingquestionsrefertomakingaprimarystandardsolutionofsodiumcarbonate,Na2CO3.

a. GivetworeasonswhyNa2CO3(s)isanexcellentprimarystandard. 2marks

b. AspartoftheprocedurewhenmakingaprimaryNa2CO3standardsolution,thechemicalisdried.

i. WhyistheNa2CO3dried? 1mark

ii. WhatistheoptimumtemperatureatwhichtodryNa2CO3(s)? 1mark

iii. Afterdrying,howwouldtheNa2CO3(s)bestoredtokeepitdry? 1mark



Question 11 (4marks)Theresultsontheagarplatesbelowindicatethatfouroutof10platesarecontaminated.

a. Identify twopossiblecausesofcontamination. 2marks

1.

2.

b. Apartfromswabbingtheworksurface,suggesttwowaysofapplyinglaboratorypracticesthatwouldassistineliminatingthecausesofcontaminationidentifiedinpart a. 2marks



Question 12 (4marks)a. Whatismeantbytheterm‘samplingplan’inasepticanalysis? 1mark

b. Whyisitimportanttofollowasamplingplan? 1mark

c. Withregardtocollectingsamples,suggesttwoplaceswheredetailedsamplinginformationcanberecorded. 2marks

1.

2.

Question 13 (4marks)Anewlogbookneedstobesetupforalaboratory’sworkingsolutions.Informationaboutallthesolutionsmustbeincludedinthelaboratorylogbook.

a. Listtwocomponentsthatmustbeincludedinthelaboratorylogbookforaworking solution. 2marks

1.

2.

b. Astocksolutionof10Mhydrochloricacidwaspurchasedfromthelocalhardwarestore.

i. Whereshouldthecontainerbestored? 1mark

ii. Whatotherpieceofinformationfromthelabelmustberecordedinthelaboratorylogbook?(Youmaynotuseanswersfrompart a.) 1mark



Question 14 (7marks)a. Whatisa‘controlblank’inmicrobiologicalanalysisandwhyisitrequired? 2marks

b. Wherecouldthecriteriaforacceptanceoftheresultsfromacontrolblankbefound? 1mark

c. Someresultsobtainedfromacontrolblankwereattheouterlimitoftheacceptableguidelines.

Suggesttwofactorsthatmayhaveresultedinthishappening.Explainhoweachfactorwouldcausetheresultsthatwereobserved. 4marks

Factor1

Explanation

Factor2

Explanation



Question 15 (4marks)Suggestwhatiscommonlytreatedbyeachofthefollowingdisposalmethods.

• Add0.5%hypochloritesolution.

• Soakin80%ethanolforfiveminutes.

• Exposetoultravioletlightinabiosafetycabinet.

• Autoclaveatthecorrecttemperatureandpressurefortherecommendedtime.

Question 16 (3marks)Maxisalaboratoryassistantwhohasjustreturnedtoworkafterbeingonsickleaveforacold.Heassumedhewouldbewellenoughtoreturntowork,despitehisoccasionalboutsofsneezing.Maxhasbeenaskedtotransferaclient’sthroatswabsamplesontomicroscopeslides.Maxhastheworkingareasetupcorrectlywiththeappropriateequipmentfortheprocedure.Heisalsowearingsafetyclothing,buthasforgottentowearafacemask.

a. WhatcouldhappentothesamplesontheslideswhenMaxpreparesthem? 1mark

b. GivenMax’sproceduraltechnique,wouldtheslidesstillbeviablesamples?Why? 1mark

c. Ifthesampleswerenotviable,whatshouldbedone? 1mark



Question 17 (9marks)Duringascheduledcheckofthelaboratorysolutions,itisfoundthatthestocksolutionof1.00Msodiumhydroxide(NaOH)usedforpreparingthesecondarystandardsolutionsisabouttoexpire.

a. Whatismeantbytheterm‘stocksolution’? 1mark

A1.00MNaOHsolutionhasbeenpreparedandatechnicianstandardisesthesolutionbytitrating 4×20mLaliquotsof1.00Mpotassiumhydrogenpthalate(KC8H5O4),aprimarystandard,withtheNaOH.NaOHreactswithKC8H5O4ina1:1ratio.Thetablebelowshowstheresultsobtainedfromthetitrationofthe1.00MKC8H5O4withthe1.00MNaOHsolution.

Titre number Titre volume (mL)

1 19.45

2 19.35

3 19.60

4 19.40

b. Calculatetheaveragetitrevalueusingconcordanttitrevalues.Showyourworking. 2marks

SECTION B – Question 17–continued


c. Usingthevaluecalculatedinpart b.,determinetheconcentration,inmol/L,oftheNaOHstocksolutiontothreesignificantfigures.Showyourworking. 3marks

d. Thelaboratoryspecificationsstatethatthestandardstocksolutionshouldbewithinarangeof5%oftheexpectedvalueof1.00MNaOH.

WouldthestandardstocksolutionofNaOHmadeupbythetechnicianbeaccepted?Justifyyouranswerusingyourresponsetopart c. 3marks

END OF QUESTION AND ANSWER BOOK

vce vet laboratory skills · 2016 vet lab skills exam 4 question 11 sterilised saline solutions are...

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