Vanderbilt Research Shared Resources and Core Facilities

Susan MeynDirector – Research Resources

VUMC Office of Research

Agenda

• Role of the Office of Research• VUMC Strategic Plan and Core Facilities• VUMC Core Facilities – Overview• How VUMC Core Facilities can support your

Research Efforts• VUMC Core Facilities – Highlights• Resources

ROLE OF THE OFFICE OF RESEARCH

Office of Research:General resources and support for research

• Point of contact for the research community both on campus and outside the institution

• Funds and coordinates development of specialized infrastructure (equipment, facilities) to support multiple research efforts

• Provides grant-writing resources including grant-ready text

• Coordinates limited submission opportunities and institutional support for selected grant programs

contact email: s.meyn@vanderbilt.edu

website: http://vanderbilt.edu/oor/

Associate Vice Chancellor for

Research

Office of Research

Shared Resources Oversight

Committee

Core Advisory Committee

Home Center or Department

Core Facility

Office of Research

Office of Research:Core Facilities and Shared Resources

• Supports scientific oversight and development of new shared resources, continued development of existing cores

• Works with research centers and programs to keep core facilities on the cutting edge by providing new equipment, recruiting technical staff.

• Coordinates with VICTR funding program to provide research support.

• Oversees and administers the Scholarship program in conjunction with NIH-funded centers to provide assistance with core service fees.

contact email: s.meyn@vanderbilt.edu

website: http://vanderbilt.edu/oor/

VUMC STRATEGIC PLAN AND CORE FACILITIES

2005–2010 VANDERBILT UNIVERSITY MEDICAL CENTER

RESEARCH ENTERPRISESTRATEGIC PLAN

http://www.vanderbilt.edu/oor/about/strategic-plan12-2005

Vanderbilt’s Research Enterprise Strategic Plan and Core Facilities

• Personalized Health & Healthcare

• Therapeutic Discovery & Translation

• Public Health & Healthcare

• Foundations of Basic Science

Leverages network of shared core facilities in Centers developed through expansion of space, faculty, and $100M investment.

2005–2010 VANDERBILT UNIVERSITY MEDICAL CENTER

RESEARCH ENTERPRISESTRATEGIC PLAN

http://www.vanderbilt.edu/oor/about/strategic-plan12-2005

• Personalized Health & Healthcare

• Therapeutic Discovery & Translation

• Public Health & Healthcare

• Foundations of Basic Science

Leverages network of shared core facilities in Centers developed through expansion of space, faculty, and $100m liquidation of quasi-endowment (2000-2005).

Vanderbilt’s Research Enterprise Strategic Plan and Core Facilities

Translation

Discovery

Implementation

Personalized Medicine• Disease susceptibility• Early detection & prevention• Variable drug actions

IntegrativescienceDisease pathwaysDrug targetsModel systems

Individual biomarkersGenomicsProteomicsMetabolomicsImaging

Research cores/shared resources

VUMC CORE FACILITIES – OVERVIEW

Medical Research Building III (MRBIII)Mass Spectrometry Resource Center Neurochemistry Core Laboratory Biomolecular Crystallography Facility Biophysical Instrumentation Core Facility Cell Imaging Shared Resource

Medical Center North (MCN)CRC–Clinical Research Center Division of Animal Care Comparative Pathology Biostatistics Collaboration Center Flow Cytometry and Immunology Cores Medical Center North (MCN)

Sleep Research Core Murine Neurobehavioral Laboratory Rat Neurobehavioral Laboratory Vanderbilt University Institute of Imaging Science

Relocating soon (VANTAGE): Genome Sciences Resource & BioVUDNA Resources

Medical Research Building IV (MRBIV)Transgenic Mouse/ESC Shared Resource VICB Chemical Synthesis Core Cell Imaging Shared Resource Light Hall

Cell Imaging Shared Resource Molecular Cell Biology Resource Cores Mouse Metabolic Phenotyping Center ZCORE - Zebrafish Aquatic Facility

Robinson Research BuildingVICB High-Throughput Screening Facility

Preston Research BuildingCancer Biostatistics Center Epidemiology Biospecimen Core Hormone Assay and Analytical Services Core VICB Protein and Antibody Resource

The Vanderbilt Clinic (TVC)Human Tissue Acquisition and Pathology Investigational Drug Services

Not shown:Computational Genomics Genetic Studies Ascertainment Survey Research Shared Resource ACCRE - Advanced Computing CenterBiomolecular NMR Facility

Overview – Vanderbilt Institutional Cores

Research core or shared resource:

• Is a research laboratory accessible to multiple investigators across disciplines.

• Provides specialized or technologically advanced laboratory services, equipment and expertise.

• Often based in an NIH funded center, e.g. the Cancer Center.

• Dedicated personnel include scientific manager/director and technical staff.

• Supported by chargebacks to investigator grants.

Typical Core Structure

Research Center or Department

Faculty – Core Scientific Director

Core Manager or Operations

Director

Core research technicians

Senior Technical Specialists

HOW VUMC CORE FACILITIES CAN SUPPORT YOUR RESEARCH EFFORTS

More than 30 institutional cores and shared resources – Whatever your research interest, there is a core facility that can

offer high end equipment access, specialized technology or advanced scientific expertise.

• $1 of every $10 funded by NIH goes to cores at Vanderbilt.• Vanderbilt is nationally recognized for the quality and

efficiency of its core facilities.• Vanderbilt is a leader in core technology development.

Scientists at all levels come to Vanderbilt because of our cores – make these valuable resources part of your research plan.

VUMC Core Facilities support your Research Efforts

Grant applications – collaborate with a core: • Plan your project and define the scope of services• Request core service fees in your budget• Do not request personnel costs – i.e. no % effort

Reviewers are very positive about using core facilities to accomplish research aims.

VUMC Core Facilities support your Research Efforts –Grant Funding

Experimental design, planning, troubleshooting: Not sure how to start?• Take a specific core training session• Attend a core seminar• Stop by the core to talk with core technical staff• Not finding the technology that you need? Contact the Office of

Research, or search the core listings.• Core facilities help you achieve your research goals.

VUMC Core Facilities support your Research Efforts –Technical Support

Unfortunately, use of research core facilities is not free! BUT there are sources of funding that can help you pay the bill:

• Join a research center - for example: Cancer Center Diabetes Center Vision Research Center Digestive Disease Research Center

• Members of research centers are eligible for core scholarships – credit vouchers that can be used to pay core service charges in supported core facilities.

• Apply for pilot project funding or micro-grant vouchers from VICTR. Investigators may receive up to $2000 every six months to pay for core services.

Support to pay core service chargesVUMC Core Facilities support your Research Efforts -

VUMC CORE FACILITIES – HIGHLIGHTS

ImagingCenter for Small Animal Imaging – MRI, PET, SPECT, CT, Ultrasound

Center for Human Studies – MRI and full range of ancillary equipment

Cell Imaging Shared Resource – Confocal laser scanning microscopes, TEM, image processing workstations

Cell Imaging Shared Resource

Cell Imaging Shared Resource

ProteomicsMass Spectrometry Resource Center – Mass Spec, Proteomics Tissue Imaging

Center for Structural Biology – NMR, X-ray crystallography, Biophysical instrumentation

Institute for Chemical Biology – High Throughput Screening and Chemical Synthesis

Olanzapine m/z 313256

N-desmethyl-m/z 299256

2-hydroxymethyl-m/z 329272

Optical image

N

N S

H

CH3

N

N

CH3

N

N S

H

CH3

N

NH

N

N S

H

N

N

CH3

OH

Khatib-Shahidi, S. et al., Anal Chem, 2006, 78, 6448http://pubs.acs.org/doi/pdf/10.1021/ac060788p

in rat, 2 hr post-dose

Mass Spectrometry Resource Center

Cell-based Assays, Animal ModelsFlow Cytometry & Immunology cores – cell surface and organelle markers, cytokine assays

Transgenic Mouse – Embryonic Stem Cell Shared Resource

Mouse and Rat Neurobehavior cores

Flow Cytometry Core

Figure 2: Distribution of memory, homing, activation and exhaustion markers. Cells from CSF (A) and blood (B) of a HIV+ patient were directly stained with a 14-colour antibody panel. Flow cytometric plots of total CD8+ T cells (in blue) are shown with HIV-specific CD8+ tetramer+ (B7-TL10) T cells on the Y axis (in green) and memory maturation markers (CD45RO, CD62L and CD127), homing markers (CCR5, VLA-4), activation markers (CD38) or markers of T cell exhaustion (PD-1 and CD57) on the X-axis. Percentages on bottom and top of each plot represent frequencies of markers on total CD8+ T cells and HIV-specific T cells respectively.

Enhanced PD-1 expression by T cells in cerebrospinal fluid does not reflect functional exhaustion during chronic human immunodeficiency virus type 1 infection.Sadagopal S, Lorey SL, Barnett L, Sutherland D, Basham R, Erdem H, Kalams SA, Haas DW.

J Virol. 2010 Jan;84(1):131-40. Epub .

CD57 PD-1

CD45RO CD62L CD127

CCR5 VLA4

CD38

MemoryMarkers

HomingMarkers

ActivationMarker

ExhaustionMarkers

CSF

37%

Blood

24%

100%

74%

100%

88%

70%

68%

50%

52%

37%

28%

4%

14%

7%

30%

CD57 PD-1

CD45RO CD62L CD127

CCR5 VLA4

CD3836%

23%

100%

39%

100%

57%

8%

7%

39%

33%

16%

12%

4%

42%

4%

45%

Tetramer+ H

IV-specific CD

8+T cellsA B

CD57 PD-1

CD45RO CD62L CD127

CCR5 VLA4

CD38

MemoryMarkers

HomingMarkers

ActivationMarker

ExhaustionMarkers

CSF

37%

Blood

24%

100%

74%

100%

88%

70%

68%

50%

52%

37%

28%

4%

14%

7%

30%

CD57 PD-1

CD45RO CD62L CD127

CCR5 VLA4

CD3836%

23%

100%

39%

100%

57%

8%

7%

39%

33%

16%

12%

4%

42%

4%

45%

Tetramer+ H

IV-specific CD

8+T cells

CD57 PD-1

CD45RO CD62L CD127

CCR5 VLA4

CD38

MemoryMarkers

HomingMarkers

ActivationMarker

ExhaustionMarkers

CSF

37%

Blood

24%

100%

74%

100%

88%

70%

68%

50%

52%

37%

28%

4%

14%

7%

30%

CD57 PD-1

CD45RO CD62L CD127

CCR5 VLA4

CD3836%

23%

100%

39%

100%

57%

8%

7%

39%

33%

16%

12%

4%

42%

4%

45%

Tetramer+ H

IV-specific CD

8+T cellsA B

GenomicsDNA Resources – extract, bank and genotype DNA

BioVU - DNA and de-identified clinical databank

Genome Sciences Resource – microarrays, next-gen sequencing (HiSeq, MiSeq)

RESOURCES

Vanderbilt University Postdoctoral Research and Shared Resources Symposium

Wednesday, May 2nd, 2012 Light Hall North Lobby and Langford Lobby

Invited Presentations: 10:30 am - 1:00 pm, LH 208* Lunch tickets provided for first 50 attendees prior to 10:30 am *

10:30 am Dr. David Piston

Louis B. McGavock Chair, Professor of Molecular Physiology & Biophysics, Physics, & Chemical & Biomolecular Engineering

11:00 am Dr. Kevin Schey Director, VVRC Proteomics Core Facility

11:15 am Dr. Daniel FosterVanderbilt Center for Neuroscience Drug Discovery “Elucidation of M5-muscarinic receptor neurophysiology in midbrain dopamine neurons using the novel allosteric modulator VU0238429”

11:30 am Dr. Christopher ColdrenOperations Director, Genomic Sciences Resource

11:45 am Dr. Xiaojing Wang Department of Biomedical Informatics “customProDB: an R package to generate customized protein database from RNA-Seq data for proteomics search”

12:00 pm 15 minute breakLunch provided for first 50 attendees in LH 208

12:15 pm Dr. Sam WellsDirector, Cell Imaging Shared Resource

12:30 pm Dr. Thomas Kehl-FieDepartment of Pathology, Microbiology & Immunology“Manganese sequestration by the host is an essential component of innate immunity”

12:45 pm 15 minute break

Poster Sessions: 1:00 pm - 3:00 pm, LH Lobby & Langford Lobby * Light refreshments will be provided in Langford Lobby*Session A, LH Lobby Session B, Langford Lobby1:00 - 2:00 pm 2:00 - 3:00 pmAnimal/Clinical Models Cell Biology Biochemistry Microbiology/ImmunologyBiological Sciences Molecular Physiology & BiophysicsCancer Biology Neuroscience

Pharmacology

Keynote Address: 3:30 pm - 4:30 pm, LH 208Dr. Gregory S. Kopf

Associate Vice Chancellor of Research Administration, University of Kansas Medical Center

Former Assistant Vice President at Wyeth Pharmaceuticals “Weathering the “Perfect Storm”: Charting the Course for a Career

in the Life Sciences ”

Awards Ceremony: 4:30 pm, LH 208 Dr. Richard Peek, 2011 Mentor of the Year

“Perspectives & Philosophies on Mentoring”Presentation of “Mentor of the Year” AwardPresentation of “Postdoc of the Year” AwardPoster awards: “Best in Category” “Best Use of Shared Resources”

Many thanks to our generous Vanderbilt sponsors!BRET Office of Postdoctoral Affairs & Roger Chalkley

Office of Research & Shared ResourcesVanderbilt Medical Alumni Association

Postdoctoral T32 Training Grant Directors

http://vanderbilt.edu/oor/

Core Listings

http://vanderbilt.edu/oor/

Grant ready text, etc

https://starbrite.vanderbilt.edu/

C.O.R.E.S.Core Ordering and Reporting Enterprise System

www.cfuis.mc.vanderbilt.edu

• Web-interface• Place orders directly with core facilities• Reserve time on core equipment• Review and act on charges made against your grants or VICTR

awards

Questions?