vanadate activation of bromoperoxidase from corallina officinalis

336 Abstracts LO84 VANADATE ACTIVATION OF BROMOPEROXIDASE FROM Corallina officinalis. H. Yu and J.W. Whittaker, Dept. of Chemistry, Carnegie Mellon University, 4400 Fifth Ave., Pittsburgh, PA 15213, USA A nonheme bromoperoxidase has been purified to homogeneity from the red seaweed Corallina officinalis. This enzyme has similar kinetic parameters to the corresponding bromoperoxidase from C. pilulifera, and similarly contains a significant amount of nonheme iron. However, - vanadate ion rather than iron activates the enzyme, and maximal activity is achieved with stoichiometric vanadium incorporation. A cor- relation between vanadium content and catalytic activity indicates that vanadium is essential to the bromoperoxidase activity. EPR spectra characteristic of V(IV) are observed on reduction, suggesting that the V is bound in a higher oxidation state complex. This enzyme thus appears to be a member of the growing class of vanadium haloperoxidases.

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Page 1: Vanadate activation of bromoperoxidase from Corallina officinalis

336 Abstracts

LO84 VANADATE ACTIVATION OF BROMOPEROXIDASE FROM Corallina officinalis. H. Yu and J.W. Whittaker, Dept. of Chemistry,

Carnegie Mellon University, 4400 Fifth Ave., Pittsburgh, PA 15213, USA A nonheme bromoperoxidase has been purified to homogeneity from

the red seaweed Corallina officinalis. This enzyme has similar kinetic parameters to the corresponding bromoperoxidase from C. pilulifera, and similarly contains a significant amount of nonheme iron. However,

vanadate ion rather than iron activates the enzyme, and maximal activity is achieved with stoichiometric vanadium incorporation. A cor- relation between vanadium content and catalytic activity indicates that vanadium is essential to the bromoperoxidase activity. EPR spectra characteristic of V(IV) are observed on reduction, suggesting that the V is bound in a higher oxidation state complex. This enzyme thus appears to be a member of the growing class of vanadium haloperoxidases.

Hyssopus officinalis

Valeriana Officinalis Valeriana Officinalis

Corallina Officinalis Alba - Campo€¦ · LABELLING: (EC REGULATION NO#1272/2008 AMENDED NO#790/2009) and Compliant to the GHS. PICTOGRAM SIGNAL WORD CODE(s): HAZARD STATEMENT CODE(s):

Calendula officinalis

Chara corallina

Australia grande barriera corallina

COLOR CREAM WITH CORALLINA OFFICINALIS · structure, by protecting it during treatment. La formulation innovante de la Crème Colorante à l’Algue Corallina, Aloe vera et theobroma

{Application of the redox system of Nocardia corallina B

Endogenous metabolism of Nocardia corallina · The Genus Nocardia The Species N. corallina The Chemical Composition of Nocardia Species Endogenous Metabolism and bActerial Survival

Vanadate increases glucocorticoid receptor-mediated gene

MarLIN · Sargassum muticum and the turfs of Corallina officinalis are considered the key characterizing and structuring species within the biotope and the sensitivity assessments

From Supramolecular Vanadate Receptors to …From Supramolecular Vanadate Receptors to Enzyme Models of Vanadium Haloperoxidase INAUGURALDISSERTATION zur Erlangung der Würde eines