uva-dare (digital academic repository) genomic diversity within … › ws › files › 25338315...

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UvA-DARE is a service provided by the library of the University of Amsterdam (http://dare.uva.nl) UvA-DARE (Digital Academic Repository) Genomic diversity within the haloalkaliphilic genus Thioalkalivibrio Ahn, A.-C.; Meier-Kolthoff, J.P.; Overmars, L.; Richter, M.; Woyke, T.; Sorokin, D.Y.; Muyzer, G. Published in: PLoS ONE DOI: 10.1371/journal.pone.0173517 Link to publication Citation for published version (APA): Ahn, A-C., Meier-Kolthoff, J. P., Overmars, L., Richter, M., Woyke, T., Sorokin, D. Y., & Muyzer, G. (2017). Genomic diversity within the haloalkaliphilic genus Thioalkalivibrio. PLoS ONE, 12(3), [e0173517]. https://doi.org/10.1371/journal.pone.0173517 General rights It is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), other than for strictly personal, individual use, unless the work is under an open content license (like Creative Commons). Disclaimer/Complaints regulations If you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: https://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. Download date: 06 Jul 2020

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Page 1: UvA-DARE (Digital Academic Repository) Genomic diversity within … › ws › files › 25338315 › journal.pone.0173517.s01… · ARh1T ALEN2T Trs.sibiricaA12T E. haloalkaliphila

UvA-DARE is a service provided by the library of the University of Amsterdam (http://dare.uva.nl)

UvA-DARE (Digital Academic Repository)

Genomic diversity within the haloalkaliphilic genus Thioalkalivibrio

Ahn, A.-C.; Meier-Kolthoff, J.P.; Overmars, L.; Richter, M.; Woyke, T.; Sorokin, D.Y.; Muyzer,G.Published in:PLoS ONE

DOI:10.1371/journal.pone.0173517

Link to publication

Citation for published version (APA):Ahn, A-C., Meier-Kolthoff, J. P., Overmars, L., Richter, M., Woyke, T., Sorokin, D. Y., & Muyzer, G. (2017).Genomic diversity within the haloalkaliphilic genus Thioalkalivibrio. PLoS ONE, 12(3), [e0173517].https://doi.org/10.1371/journal.pone.0173517

General rightsIt is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s),other than for strictly personal, individual use, unless the work is under an open content license (like Creative Commons).

Disclaimer/Complaints regulationsIf you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, statingyour reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Askthe Library: https://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam,The Netherlands. You will be contacted as soon as possible.

Download date: 06 Jul 2020

Page 2: UvA-DARE (Digital Academic Repository) Genomic diversity within … › ws › files › 25338315 › journal.pone.0173517.s01… · ARh1T ALEN2T Trs.sibiricaA12T E. haloalkaliphila

99

99

99

99

99

99

99

99

9987

83

99

99

79

92

69

97

99

99

99

97

99

97

92

98

99

99

99

99

99

99

99

99

99

99

8298

86

6099

99

63

82

82

94

79

9995

0.05

ALJ11ALJ2ALJ8ALJ12T

ALgr1ALRhARh4ALJ4ALJ5AL5ALJ9

ALJ15K90mix ALJ20 ALJ21AL21HL-Eb18ARh3

ARh2T

ARh5ALJ3 ALD1ALJ10

ALJTALE25ALE21 ALE12ALE28ALE30ALE31ALE17ALE16ALE27ALE9ALE22

ALJ16ALJ1

ALJ6ALJ7

AKL11ALE14ALE6

AKL19ALMg11ALM2T

AKL9AKL10AKL3ALMg2ALMg13-2

AKL12AKL6AKL7AKL8AL2T

ALMg3ALMg9

AKL17ALgr5

ALR17-21HL17T

ALJ24ALSr1

ALE18ALE10

ALE11ALE20ALE23ALgr3ALE19

ARh 1T

ALEN2T

Trs. sibirica A12T

E. haloalkaliphila ATCC 51935T

ALJ17HL-EbGr7 T

ARhD 1T

ALJDT

Ths. halophila HL3T

H. halophila SL1T A. ehrlichii MLHE-1T

atpD

Page 3: UvA-DARE (Digital Academic Repository) Genomic diversity within … › ws › files › 25338315 › journal.pone.0173517.s01… · ARh1T ALEN2T Trs.sibiricaA12T E. haloalkaliphila

ALE27ALE22ALE28ALE25ALE21ALE30ALE12ALE17ALE9ALE16ALE31ALJT

AKL19ALE14ALE6ALMg11AKL6AKL7AKL10ALM2T

ALMg13-2AKL12AKL3AKL9ALMg2ALMg3AKL8ALMg9AL2T

ALJ16ALJ1ALJ6ALJ7

AKL11ALD1ALJ10ALJ3ARh3ARh2T

ARh5ALJ21AL21ALJ20HL-Eb18 K90mix

AL5ALJ9ALJ5ALJ4ARh4ALgr1 ALJ2ALRhALJ12T

ALJ8 ALJ11ALJ15

ALSr1ALJ24

ALE23ALE19 ALgr3ALE18ALE10ALE20ALR17-21HL17T

ALE11AKL17ALgr5

ARh 1T

ALEN2T

Trs. sibirica A12T

E. haloalkaliphila ATCC 51935T

ALJ17HL-EbGr7T

ARhD 1T

ALJDT Ths. halophila HL3T

H. halophila SL1T

A. ehrlichii MLHE-1 T

100

100

100

100

100

100

68100

100

99

95

10092

10099

100

97100

100

100

80100

100

10081

100

79

78

100

100

100

100

100

88

72

91

7269

92

10098

66

99

66

98

100

100

100

100

100 67

66

82

100

100

6399

0.05

clpA

Page 4: UvA-DARE (Digital Academic Repository) Genomic diversity within … › ws › files › 25338315 › journal.pone.0173517.s01… · ARh1T ALEN2T Trs.sibiricaA12T E. haloalkaliphila

ALMg9AKL7ALMg3AL2T

ALMg2AKL3AKL9AKL8ALMg13-2AKL12AKL10AKL6

ALM2T

ALMg11ALE14ALE6AKL19

ALE27ALE22ALE17ALE16ALE30ALE12ALE31ALE9ALE28ALE25ALE21

ALJTALJ16

ALJ1ALJ6ALJ7

AKL11ARh2T

ARh5ARh3ALD1ALJ10ALJ3HL-Eb18K90mixALJ21AL21ALJ20ALJ5AL5ALJ4ARh4

ALgr1ALJ2ALJ9ALJ15ALRhALJ12T

ALJ8ALJ11

ALJ24ALSr1

AKL17ALgr5

ALR17-21ALE11HL17T

ALE19ALgr3ALE20ALE18ALE23ALE10

ARh 1T

ALEN2T

E. haloalkaliphila ATCC 51935T

Trs. sibirica A12T

ALJDT

ARhD 1T

ALJ17HL-EbGr7T

Ths. halophila HL3T

H. halophila SL1T A. ehrlichii MLHE 1T

10054

100

10085

100

100

10098

8572

77

100

90

71

69

99

82

100

100100

100

100

100

100

99

99

7796

95

98

100

100

99

100

83

81

80

97

97

99

616181

100

100

99

100

87

100

99

96

7278

81

6073

0.1

dnaJ

Page 5: UvA-DARE (Digital Academic Repository) Genomic diversity within … › ws › files › 25338315 › journal.pone.0173517.s01… · ARh1T ALEN2T Trs.sibiricaA12T E. haloalkaliphila

AKL8AL2T

ALMg3ALMg9AKL10AKL6AKL7AKL12AKL3AKL9ALMg13-2ALMg2ALM2T

ALMg11AKL19ALE12

ALE31ALE30ALE17

ALE28ALE16ALE27ALE22ALE9ALE25ALE21

ALE14ALE6

ALJ1ALJ6ALJ7ALJT

ALJ16AKL11 ALD1ALJ10ALJ3

ARh3ARh2T

ARh5ALJ21AL21ALJ20HL-Eb18K90mix ALJ5AL5ALJ4ARh4ALJ9ALRhALgr1ALJ15ALJ2ALJ12T

ALJ8ALJ11ALJ24ALSr1

AKL17ALgr5ALR17-21HL17T

ALE23ALE19ALE18ALE10ALE11ALE20 ALgr3

ARh 1T

ALENT

Trs. sibirica A12T

E. haloalkaliphila ATCC 51935T

ALJDT ARhD 1T

ALJ17HL-EbGr7T

Ths. halophila HL3T

H. halophila SL1T

A. ehrlichii MLHE-1T

10077

98

100

62100

100

97

100100

7398

100

100

659295

100100

100

100

100100

100

100

100

9494

65

80

66

100

100

100

100100

100

100

10060

86

88

60

100

91

99

95

100

97

100

77

67

100

100

100

72100

100

61

94

0.1

gyrB

Page 6: UvA-DARE (Digital Academic Repository) Genomic diversity within … › ws › files › 25338315 › journal.pone.0173517.s01… · ARh1T ALEN2T Trs.sibiricaA12T E. haloalkaliphila

AKL6AKL7AL2T

ALMg9ALMg3AKL8AKL12ALMg13-2ALMg2AKL10AKL3AKL9

ALM2T

ALMg11AKL19ALE14ALE6ALE9ALE17ALE25ALE21ALE31ALE30ALE28ALE12ALE16ALE27ALE22

ALJ10ALD1ALJ3HL-Eb18K90mixARh3ARh2T

ARh5ALJ20ALJ21 AL21

AKL11ALgr1 ALRh ALJ2 ALJ15ALJ8ALJ11ALJ12T

ARh4ALJ4ALJ5AL5ALJ9ALJ16ALJTALJ1ALJ6ALJ7

ALSr1ALJ24ALR17-21AKL17ALgr5HL17T

ALgr3ALE19ALE11ALE23ALE20ALE18ALE10

ARh 1T

ALEN2T

Trs. sibirica A12T

E. haloalkaliphila ATCC 51935T

ALJDT

ARhD 1T

ALJ17HL-EbGr7T

Ths. halophila HL3T H. halophila SL1T

A. ehrlichii MLHE-1T

10063

100

100

94

100

100

10095

99

75

94

96

98

100100

100

100

100

100

100

99

97

96100

100

100

100

100

65

65

90

99

81

100

97

100

75

72

83

69

100

99

100

89

97

0.1

rpoD

Page 7: UvA-DARE (Digital Academic Repository) Genomic diversity within … › ws › files › 25338315 › journal.pone.0173517.s01… · ARh1T ALEN2T Trs.sibiricaA12T E. haloalkaliphila

ALE31ALE22ALE27ALE25ALE21ALE17ALE28ALE30ALE16ALE9ALE12

ALJ1ALJ6ALJ7

AKL19ALE14ALE6ALMg11

AKL8AL2T

ALMg9ALM2T

AKL7AKL6ALMg2AKL10ALMg13-2AKL3AKL12ALMg3AKL9ALJT

ALJ16AKL11

ARh2T

ARh5ARh3

ALJ21AL21ALJ20ALJ15K90mix HL-Eb18ALJ3ALD1ALJ10AL5ALJ9ALJ5ALJ4ARh4ALJ8ALJ11ALJ12T

ALgr1ALJ2ALRh

ALJ24ALSr1

AKL17ALE21HL17T

ALR17-21ALE19

ALE20ALE23ALgr3ALE11ALE18ALE10ARh 1T

ALEN2T

Trs. sibirica A12 T

E. haloalkaliphila ATCC 51935T

ARhD 1T

ALJDT

ALJ17HL-EbGr7T

H. halophila SL1T

A. ehrlichii MLHE-1T

Ths. halophila HL3T

100100

100

6299

99

100

100

9599

89

75

64

81

77

100

100

100

95100

100

89

96

100

100

100

89

9990

96

100

100

100

89

98

98

100

100

100

7484

6688

100

99

100

9894

75

100

77

0.1

rpoH

Page 8: UvA-DARE (Digital Academic Repository) Genomic diversity within … › ws › files › 25338315 › journal.pone.0173517.s01… · ARh1T ALEN2T Trs.sibiricaA12T E. haloalkaliphila

AKL10AL2T

ALMg13-2AKL12ALMg2AKL3AKL9ALMg3ALM2T

AKL6ALMg9AKL8AKL7

ALMg11ALJ1ALJ6ALJ7

ALE14ALE6

AKL19ALE9ALE28ALE17ALE30ALE12ALE27ALE22 ALE31ALE25ALE21ALE16

ALJ16ALJT

ARh2T

ARh5ARh3

ALJ3ALD1ALJ10

AKL11ALJ21AL21ALJ20HL-Eb18K90mix ALJ2AL5ALJ9ALJ5ALJ4ARh4ALgr1ALJ12T

ALJ8ALJ11ALRhALJ15

ALJ24ALSr1ALR17-21AKL17ALgr5HL17T

ALE18ALE10ALE11ALE20ALE23ALE19ALgr3ARh 1T

ALEN2T

Trs. sibirica A12T

E. haloalkaliphila ATCC 51935T

ALJDT

ARhD 1T

ALJ17HL-EbGr7T

Ths. halophila HL3T

A. ehrlichii MLHE-1T

H. halophila SL1T

100

95

9899

100

100

74100

100

76100

68

100

65

100

100

8599

100

100

6186

100

100

78

98

100

99

70

99

100

6999

100

99

93

99

92

79

100100

100

100

77

63

0.2

rpoS

Page 9: UvA-DARE (Digital Academic Repository) Genomic diversity within … › ws › files › 25338315 › journal.pone.0173517.s01… · ARh1T ALEN2T Trs.sibiricaA12T E. haloalkaliphila

ALMg2AKL9AKL3ALMg3AKL10 AKL12ALMg13-2ALMg9AL2T

ALM2T

AKL6AKL8AKL7ALMg11

AKL19ALE14ALE6

ALJ1ALJ7ALJ6

ALE27ALE22ALE28ALE25ALE21ALE16ALE12ALE17ALE9

ALE31ALE30

ALJ16ALJTAKL11

ARh2T

ARh5ARh3ALD1ALJ10ALJ3

ALJ21AL21ALJ20HL-Eb18K90mix

AL5ALJ9ALJ5 ALJ4ARh4ALJ2ALgr1ALRhALJ12T

ALJ8ALJ11ALJ15

ALJ24ALgr5ALSr1AKL17ALE18ALE10ALgr3ALE20ALR17-21ALE11HL17T

ALE23ALE19

ARh 1T

ALEN2T

Trs. sibirica A12T

E. haloalkaliphila ATCC 51935T

ALJ17HL-EbGr7T

ARhD 1T

ALJDT Ths. halophila HL3T

H. halophila SL1T

A. ehrlichii MLHE-1T

99

99

100

91100

100

908696

77

67

100

99

99

100

100

67100

100

100

61

83100

100

71

100

67

62

76

87

100

100

100

100

97

100

100

100

64

75

100

99

98

93

100

64

64

100

99

9960

87

100

0.1

secF