uv / vis spectrophotometer - emclab.de · uv / vis spectrophotometer emc-11-uv. emc-11-uv...

EMCLAB Instruments GmbH

A Professional Manufacturer

UV / VIS Spectrophotometer

E-Mail: [email protected] | www.emc-lab.de

EMCLAB Instruments GmbH Bismarckstrasse 120

47057 Duisburg | Germany Phone: +49 203 3064042

Fax: +49 203 3064044

E-mail: [email protected] Web: www.emc-lab.de

Contact: Mrs. Anke Rehmann, Managing Director

mailto:[email protected]

http://www.emc-lab.de/

E-Mail: [email protected] | www.emc-lab.de 3

TRACEABLE

EMCLAB Spectrophotometer

Selection guide

Model

Optical System

Wavelength Range

Bandwidth

EMC-11D-V

Single Beam

325-1000 nm

4 nm

EMC-11-UV

Single Beam

200-1000 nm

4 nm

EMC-16PC-V

Single Beam

320-1100 nm

4 nm

EMC-16PC-UV

Single Beam

190-1100 nm

4 nm

EMC-31PC-UV

Single Beam

190-1100 nm

2 nm

EMC-32PCS-UV

Single Beam Scanning function

190-1100 nm

0.5/1/2/4/5 nm

EMC-61PC-UV

Double Beam

190-1100 nm

1.8 nm

EMC-61PCS-UV

Double Beam Scanning function

190-1100 nm

0.5/1/2/4/5 nm

EMC-NANO-UV

Single Beam 0.2 ~ 2.0 µl

190-1100 nm

4 nm

“PC” means with PC software, Standard central beam height: 15 mm M. Wave Professional PC-Control Software included in: EMC-16PC-V, EMC-16PC-UV UV/VIS Analyst Software included in: EMC-31PC-UV, EMC-31PCS-UV EMC-61PC-UV, EMC-61PCS-UV EMCLAB Spectrophotometers are tested with DAkkS* certified UV/VIS Reference Materials NIST traceable:

Photometric Accuracy

Wavelength Accuracy

Spectral Resolution

Stray Light *Accreditation body for the Federal Republic of Germany

4 E-Mail: [email protected] | www.emc-lab.de

Visible Spectrophotometer EMC-11D-V

EMC-11D-V is the only model of manually setting wavelength, but precise design and high quality components ensures excellent performance. It is widely used in high schools and colleges for general analysis and experiments. Features:

Large LCD screen (128*64 Dots)

Wavelength can be read out from the screen directly

Auto Zero and Blank

Parallel port, printed directly

Large sample compartment, it can be accommodate 5-100 mm path length cuvettes with optional holders

Pre-aligned design ensures the user can change lamp conveniently

The optional PC software M. Wave Basic based on Windows can be expand the applications to Standard Curve & Kinetics

High quality silicon photometric diode detector and 1200 lines/mm grating ensure high accuracy and precision

Model

EMC-11D-V

Wavelength Range 325-1000 nm Spectral Bandwidth 4 nm Optical System Singe Beam, grating 1200 lines/mm Wavelength Accuracy ±2 nm Wavelength Repeatability 1 nm Photometric Accuracy ≤±0.5 % T or ±0.003A@1A Photometric Range 0-200 % T, -0.3 - 3A, 0-1999 Conc Stray Light 0.3 % T Stability ±0.004A/h @500 nm Display 128*64 LCD Photometric Mode T, A, C, F Detector Silicon Photodiode Standard Cell Holder 4-position 10 mm cell changer Sample compartment Standard 10 mm path length cuvette Light Source Tungsten lamp Output USB Port & Parallel Port (Printer) Power Requirement AC 85V~265V 50/60 Hz Dimensions (LxWxH) 489 x 387 x 180 mm Weight 8 kg


UV / VIS Spectrophotometer EMC-11-UV

EMC-11-UV spectrophotometer is the ideal instrument for education and QC laboratories. Using your standard sample solutions, you can get a standard curve on the large LCD screen. It is widely used in colleges and enterprises for general quantitative analysis and experiments. Features:


Can display total 50 groups of data, 3 groups per screen. Can display standard curve and the curve equation

System can also save the test results. Total groups of data and 200 standard curves can be saved; it is convenient for check and reload.

Data can be restored after a sudden power cut

Auto setting wavelength

Tungsten lamp & Deuterium lamp can be turned on/off individually to extend lifetime

Pre-aligned design makes it convenient to change lamps

Large sample compartment, it can be accommodate 5-100 mm path length cuvettes with optional holders. A variety of optional accessories are available

The optional application software M. Wave Professional provides complete control of the spectrophotometer through the Built-in USB port. You can achieve the following functions: I. Quantitative II. Kinetics III. Wavelength Scan IV. Multi Wavelength V. DNA/Protein

Model

EMC-11UV

Wavelength Range 200-1000 nm Spectral Bandwidth 4 nm Optical System Singe Beam, grating 1200 lines/mm Wavelength Accuracy ±2 nm Wavelength Repeatability 1 nm Photometric Accuracy ≤±0.5 % T or ±0.003A@1A Photometric Range 0-200 % T, -0.3 - 3A, 0-9999 Conc. Stray Light 0.3 % T Stability ±0.002A/h @500 nm Detector Silicon Photodiode Standard Cell Holder 4-position 10 mm cell changer Sample compartment Standard 10 mm path length cuvette Light Source Tungsten & Deuterium lamp Output USB Port & Parallel Port (Printer) Power Requirement AC 110/220V 50/60 Hz Dimensions (LxWxH) 489 x 387 x 180 mm Weight 14 kg


UV / VIS Spectrophotometer EMC-16 series

EMC-16 series are simple-to-use instruments with advanced performance, its stray light is only 0.05%T. The local stand-alone software provides functions of Photometry, Quantitative Test, Kinetics and System Utilities functions. Features:


System can also save the test results. Total 200 groups of data 200 standard curves can be saved in the RAM. Convenient for check and reload.

Data can be restored after a sudden power cut

Auto setting wavelength

Tungsten lamp & Deuterium lamp can be turned on/off individually to extend lifetime

Pre-aligned design makes it convenient to change lamps

Large sample compartment, it can be accommodate 5-100 mm path length cuvettes with optional holders. A variety of optional accessories are available

The application software M. Wave Professional provides complete control of the spectrophotometer from a computer through the Built-in USB port. It can expand to the following functions: I. Quantitative, II. Kinetics, III. Wavelength Scan, IV. Multi Wavelength, V. DNA/Protein

Functions: 1. Photometry, 2. Quantitative, 3. Kinetics, 4. System Utilities Model

EMC-16PC-V

EMC-16PC-UV

Wavelength Range 320-1100 nm 190-1100 nm Spectral Bandwidth 4 nm Optical System Singe Beam, grating 1200 lines/mm Wavelength Accuracy ±0.5 nm Wavelength Repeatability 0.3 nm Photometric Accuracy ≤±0.5 % T or ±0.003A@1A Photometric Range 0-200 % T, -0.3 - 3A, 0-9999 Conc. Stray Light ≤0.05 % T@360 nm ≤0.05 % T@260, 360 nm Stability ±0.002A/h @500 nm Display Graphic LCD (128+64 dots) Keyboard 22 Membrane keypad Standard Cell Holder 4-position 10 mm cell changer Sample compartment Standard 10 mm path length cuvette Light Source Tungsten lamp Tungsten & Deuterium lamp Output USB Port & Parallel Port (Printer) Power Requirement AC 110/220V 50/60 Hz Dimensions (LxWxH) 470 x 373 x 187 mm Weight 12 kg 14 kg



Series Local Control Software Main Menu Move the cursor on the function menu you want, then press ENTER key to go into the corresponding interface

1. Basic Mode Absorbance, Transmittance or Concentration measurements. It can display and save 200 groups of data, 5 groups per screen

2. Quantitative 2.1 Standard Curve A most 9 standard sample can be used to establish a standard curve. The curve and its equation will be displayed on the screen simultaneously. You can measure your unknown concentration solutions by the curve. Total 200 curves can be saved in the RAM 2.2 Coefficient Method If the coefficient k & b in the formula C=k*a + b is known, you can input them directly by the button, and then test your unknown solutions.

3. Kinetics This mode may be used for time course scanning or reaction rate calculation. Abs. VS Time graphs is displayed on the screen in real time. It can most record 1000 data.

4. System Utilities Lamp management, time & data set, obtain dark current, calibrate wavelength, default system, and some other system functions.



The EMC-3 series are advanced single beam spectrophotometers. They provide excellent performance for measurements in the range of 190 to 1100 nm. The memory is 32K. They are suitable for pharmaceutical, biochemical and clinical lab applications as well as routine applications such as quantitative analysis, kinetics, wavelength scan, multi-wavelength and DNA/Protein analysis. The UV/VIS Analyst software based on Microsoft Windows® makes the instruments versatile. The instruments provide excellent performance for measurements. Features:

Fixed or variable slits (Bandwidth)

Sealed, solvent-resistant tactile keypad with alpha-numeric entry for file names and units

Pre-aligned deuterium lamp for easy lamp replacement. The status of the lamp may be monitored

Online software upgrading via internet helps to keep it updated

Data Download-to-PC software expands the data storage to unlimited

The PC model comes standard with Windows® based application software UV-VIS Analyst

Data can be saved by USB memory device directly Model

EMC-31PC-UV

EMC-32PCS-UV

Wavelength Range 190-1100 nm Spectral Bandwidth 2 nm 0.5/1/2/4/5 nm Optical System Single Beam, grating 1200 lines/mm Wavelength Accuracy ±0.5 nm ±0.3 nm Wavelength Repeatability 0.3 nm 0.2 nm Scan Speed Hi, MED, LOW, MAX. 3000 nm/min Photometric Accuracy ≤±0.5 % T or ±0.005A@1A Photometric Range 0-200 % T, -0.3 - 3A, 0-9999Conc. Stray Light 0.05 % T@220, 340 nm Stability ±0.002A/h @500 nm Display 5 inches LCD (320*240 dots) Baseline Flatness ±0.002A (200-1000 nm) Standard Cell Holder 4-position 10 mm cell changer Sample compartment Standard 10 mm path length cuvette Light Source Tungsten & Deuterium lamp (Pre-aligned)

Output

USB type A port for USB memory device (Right side)

USB type B port for optional computer connectivity (Back)

Parallel port for printer

Power Requirement AC 110/220V 50/60 Hz Dimensions (LxWxH) 491 x 365 x 180 mm 579 x 428 x 198 mm Weight 14 kg 20 kg



The EMC-6 series are advanced double beam spectrophotometers. The two detectors measure sample and reference respectively and simultaneous for optimizing measurement accuracy. They provide excellent performance for measurements in the range of 190 to 1100 nm. The memory is 32K. They are suitable for pharmaceutical, biochemical and clinical lab applications as well as routine applications such as quantitative analysis, kinetics, wavelength scan, multi-wavelength and DNA/Protein analysis. The UV/VIS Analyst software based on Microsoft Windows® makes the instruments versatile. The instruments provide excellent performance for measurements. Features:

Fixed or variable slits (Bandwidth)

Sealed, solvent-resistant tactile keypad with alpha-numeric entry for file names and units

Pre-aligned deuterium lamp for easy lamp replacement. The status of the lamp may be monitored

Online software upgrading via internet helps to keep it updated

Data Download-to-PC software expands the data storage to unlimited

The PC model comes standard with Windows® based application software UV-VIS Analyst

Data can be saved by USB memory device directly Model

EMC-61PC-UV

EMC-61PCS-UV

Wavelength Range 190-1100 nm Spectral Bandwidth 1.8 nm 0.5/1/2/4/5 nm Optical System Double Beam, grating 1200 lines/mm Wavelength Accuracy ±0.3 nm Wavelength Repeatability 0.2 nm Scan Speed Hi, MED, LOW, MAX. 3000 nm/min Photometric Accuracy ≤±0.5 % T or ±0.005A@1A Photometric Range 0-200 % T, -0.3 - 3A Stray Light 0.05 % T@220, 340 nm Stability ±0.001A/h @500 nm Display 5 inches LCD (320*240 dots) Baseline Flatness ±0.001A Standard Cell Holder Standard 10 mm single cell holder (2 pcs.) Sample compartment Standard 10 mm path length cuvette Light Source Tungsten & Deuterium lamp (Pre-aligned) Output

USB type A port for USB memory device (Right side)

USB type B port for optional computer connectivity (Back)

Parallel port for printer

Power Requirement AC 110/220V 50/60 Hz Dimensions (LxWxH) 589 x 428 x 200 mm Weight 22 kg


NANO Spectrophotometer EMC-NANO-UV

EMC-NANO-UV the new model for fast, easy, accurate and economic measuring of minimum volumes of 0.2 µl to 2 µl with high quality components ensures excellent performance. It is used in DNA, PCR analyzing within the range from UV to VIS range. Features:

Large 5’’ LCD screen (320*240 Dots)

0.2 µl sample volume for DNA, PCR, protein …

One step – one movement drop close turn and measure

Wavelength from 190-1100 nm

Pre-aligned design ensures the user can change lamps conveniently

Extended output USB A and USB ports for software and printer

High quality silicon photometric diode detector and 1200 lines/mm grating ensure high accuracy and precision

Model

EMC-NANO-UV

Wavelength Range 190-1100 nm Spectral Bandwidth 4 nm Optical System Singe Beam, grating 1200 lines/mm Wavelength Accuracy ±0.5 nm Wavelength Repeatability 0.3 nm Photometric Accuracy ≤±0.5 % T or ±0.004A@1A Photometric Range 0-200 % T, -0.3 - 3A, 0-9999 Conc. Stray Light 0.05 % T@220, 340 nm Stability ±0.0008A/h @500 nm Detector Silicon Photodiode Multifunctional Cell Unit 0.5 – 2 µl Volume Scan speed Hi, MED, LOW – MAX: 3000nm/min Light Source Tungsten & Deuterium lamp Output 2 USB Port & Parallel Port (Printer) Power Requirement AC 110/220V 50/60 Hz Dimensions (LxWxH) 491 x 365 x 180 mm Weight 14 kg

Recommended Pipette Article No. Volume (µl) Increment (µl) EMC-5720005 0.1-2.5 0.05


UV / VIS Spectrophotometer EMC-3 & EMC-6 series

Series Local Control Software

All methods are included as built-in standard; this eliminates the need of software. Online software update via internet. The local control software includes functions such as: Photometry, Quantitative, Kinetics, Wavelength Scan, Multi-wavelength Test and System Utilities

Standard Curve Up to 10 standard solutions may be used to establish calibration equation curve. There is a choice of four methods for fitting curve through the calibration points: linear fit, Linear fit through zero, square fit and cubic fit.

Wavelength Scan The wavelength Scan intervals are 0.1, 0.2, 0.5, 1, 2, 5 nm and High, Medium and Low scan speeds are available. Scan speeds vary from 100 to 3000 nm/min. Wavelengths are scanned fro high to low so that the instrument stand-by at high wavelength. This minimizes the degradation of UV sensitive samples. Precise control of filter and lamp changes means that their effects are not seen on the final can. Post-run manipulation includes re-scaling axes, curve tracking and peak picking.

Kinetics This mode may be used for scanning time course or reacting rate calculations. Abs. VS time graphs is displayed on the screen in real time. Wait time and measurement time up to 12 hours may be entered with time intervals of 0.5, 1, 2.5, 10, 30 seconds and 1 min. Post-run manipulation includes re-scaling, curve tracking and selection of the part of the curve required for the rate calculation. Rate is calculated using a linear regression algorithm before multiplying by the entered factor.

Multi-Wavelength Up to 10 wavelengths may be entered, allowing the measurement of multiple wavelengths on a series of samples.

DNA/Protein Test Concentration and DNA purity are calculated by Absorbance ratios 260 nm/280 nm or 260 nm/230 nm with optional subtracted absorbance at 320 nm DNA concentration = 62.9*A260-36.0*A280 or 49.1*A260-3.48*A230 Protein concentration = 1552*A260-757.3*A280 or 183*A260-75.8*A230 Other wavelengths and factors may be entered.


Spectrophotometer Software

M. Wave Professional PC-Control Software for EMC-16 series

M. Wave Professional application software is based Microsoft Windows, the instrument can be controlled by PC software through built-in USB communication port, which makes the UV/VIS series with more functions and easy to control. Quantitative Use up to 20 standards to establish standard curve. Three methods for fitting a curve:

1. Linear fit 2. Linear through zero 3. Square fit

Kinetics The Kinetics mode may be used for scanning time course or reacting rate calculations. Abs. VS time graphs is displayed.

Wavelength Scan Automatically records peaks and valleys. The quantity of the curves stored is unlimited. Post-run manipulation and processing includes

1. Re-scaling axes, curve 2. Smoothing, combination, zooming, overlap… 3. 1

st to 4

th derivative

Multi-wavelength Test You can set up to 20 wavelengths to measure a sample

DNA/Protein Test Optional two formulas: DNA concentration = 62.9*A260-36.0*A280 or 49.1*A260-75.8*A230 You can also enter other wavelengths and factors to calculate.



UV/VIS Analyst Software for EMC-3 series & EMC-6 series

The PC application software UV/VIS Analyst takes the features of the stand-alone version plus more powerful data processing, expanded data collecting, and storage capability. It comes standard with EMC-3 and 6 series. The PC application software offers:

1. Photometric Mode 2. Quantitative Test (Standard curve) 3. Wavelength Scan 4. Kinetics 5. DNA/Protein 6. Multi-Wavelength 7. System Utility

Quantitative Test Use up to 20 standards to establish standard curve. Four methods for fitting a curve:

1. Linear fit 2. Linear through zero 3. Square fit 4. Cubic fit

Wavelength Scan Automatically records peaks and valleys. The quantity of channels is unlimited. You can simultaneously store as many as desired. Post-run manipulation and processing includes:

1. Re-scaling axes, curve 2. Smoothing, combination, zooming, overlap… 3. 1

st to 4

th derivative

Kinetics (Abs. VS time) The Kinetics mode may be used for scanning time course or reacting rate calculations. Abs. VS time graphs are displayed on the screen in real time. Wait time, measurement time and time intervals may be entered. Post-run manipulation includes re-scaling, curve tracking and selection of the part of the curve required for the rate calculation. Rate is calculated using a linear regression algorithm before multiplying by the entered factor.



DNA/Protein Test Concentration and DNA purity are quickly and easily calculated. Absorbance ratios 260 nm/280 nm with optional subtracted absorbance at 320 nm DNA concentration = 62.9*A260-36.0*A280 Protein concentration = 1552*A260-757.3*A280 Other wavelengths and factors may be entered.

Multi-wavelength Up to 20 wavelengths can be selected and multiple samples can be measured. (auto cell changer is required to run multiple samples automatically)


Spectrophotometer Cuvettes

Cuvettes Standard Premium

Square

Glass 10 mm

EMC-S0001

EMC-100OS

Glass 50 mm

EMC-S0002

EMC-105OS

Quartz glass 10 mm

EMC-S0003

EMC-100QS

Quartz glass 50 mm

EMC-S0004

EMC-105QS

Micro

Semi-micro cell glass 1000 µl

EMC-S0005

EMC-108OS

Semi-micro cell quartz glass 1000 µl

EMC-S0006

EMC-108QS

Semi-micro cell quartz glass 1000 µl (black side walls and base)

n/a

EMC-108QSB

Micro cell quartz glass 500 µl

EMC-S0007

EMC-108QSM

Micro cell quartz glass 500 µl (black side walls and base)

n/a

EMC-108QSMB

Ultra-micro cell quartz glass 100 µl

EMC-S0008

EMC-108QSM15

Flow-through

Flow-through cell glass 10 mm

EMC-S0009

EMC-175OS

Flow-through cell quartz glass 10 mm

EMC-S0010

EMC-175QS


Spectrophotometer Reference Materials

UV/VIS certified reference materials

EMC-SET-667UV Complete liquid filter set for checking the spectrophotometer acc. to European Pharmacopeia

Absorbance

Wavelength accuracy

Stray light

Spectral resolution The set consists of eight liquid filters. They are designed for use with the standard 100 mm cell holder provided with spectrophotometers. The set is supplied in a Pelicase. For identification purpose a number is engraved on each filter. The absorbance values and/or peak position wavelengths of every Filter are quoted in the accompanying calibration certificate. ____________________________________________________________________________________ EMC-SET-666 Complete glass filter set for testing Absorbance Wavelength accuracy The set consists of five glass filters and is supplied in a Pelicase Holmium Oxide Filter Neutral Density Glass Filter 0.25 Abs. Neutral Density Glass Filter 0.5 Abs. Neutral Density Glass Filter 1.0 Abs. Empty Filter Mount, Aluminium frame ____________________________________________________________________________________ EMC-SET-666-01 Complete glass filter set for testing Absorbance Wavelength accuracy The set consists of three glass filters and is supplied in a Pelicase Didymium Glass Filter Neutral Density Glass Filter 0.5 Abs. Neutral Density Glass Filter 1.0 Abs. ____________________________________________________________________________________ EMC-SET-666-02 Complete glass filter set for testing Absorbance Wavelength accuracy The set consists of three glass filters and is supplied in a Pelicase Holmium Oxide Filter Neutral Density Glass Filter 0.5 Abs. Neutral Density Glass Filter 1.0 Abs.

EMC-SET-667UV EMC-SET-666

TRACEABLE


Spectrophotometer Accessories

Cell Holder 4-cell holder for 10 mm square cuvette, standard 4-cell holder for up to 50 mm square cuvette Cell holder for micro cells, central beam height: 15 mm Single cell holder Single cell holder, water-jacketed 4-cell holder, water-jacketed

Peltier Systems

Peltier System, Constant-Temperature-System, holder beam height: 15 mm Sipper System, holder beam height: 15 mm Peltier /Sipper System, Constant-Temperature System, holder beam height: 15 mm

Lamps

Halogen lamp 6V10W (only for EMC-11D-V) Halogen lamp 12V20W Halogen lamp 12V20W (only for EMC-6 series) Deuterium lamp for UV Other types on request | Technical changes reserved


Index

Product Description Beam Page

EMC-11D-V VIS Single 4

EMC-11-UV UV/VIS Single 5

EMC-16PC-V VIS Single 6

EMC-16PC-UV UV/VIS Single 6

EMC-31PC-UV UV/VIS Single 8

EMC-32PC-UV UV/VIS Single /Scanning 8

EMC-61PC-UV UV/VIS Double 9

EMC-61PCS-UV UV/VIS Double/Scanning 9

EMC-NANO-UV UV/VIS Single 10

EMC-M.Wave Software PC Professional For EMC-16 series 12

EMC-UV/VIS Analyst Software PC For EMC-3 and EMC-6 series 13

EMC-cuvettes Glass and Quartz 10 mm, 50 mm, Standard/Premium 15

EMC-SET/UV/VIS Certified reference materials DAkkS certified/ NIST traceable 16

EMC-Spectrophotometer Accessories 17


EMC-Spectrophotometers will be delivered with Accuracy Calibration Certificate. Reference Materials for this calibration are DAkkS (German body for accreditation) calibrated and NIST traceable.

EMCLAB Instruments GmbH Bismarckstrasse 120 47057 Duisburg | Germany Fon: +49 203 3064042 Fax: +49 203 3064044 E-mail: [email protected] Web: www.emc-lab.de

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