using the microscope rules for using the microscope binocular head
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Using the MicroscopeRules for Using the
Microscope1. Use two hands to
carry a microscope,one hand holding thearm, the other holdingthe base.
2. Only use lens paperand water to clean thelenses.
Parts of theMicroscope
eyepieces
bodyrevolvingnosepiecemechanical stage
light source
base
Parts of theMicroscope
fine focusadjustment knob
arm
coarse focusadjustment knob
Parts of theMicroscope
light switch
mechanical stagepositioning knobs
objective lenseson revolvingnosepiece
binocular headwith eyepieces
Binocular Headinterpupillary scale window
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Setting theCalibrated
Diopter Ring
diopter ring
set line
Revolving Nosepiece
Objectives
Scanning Objective: 4X
Low Power Objective: 10X
High Power Objective: 40X
Oil Immersion Objective: 100X
Rules for Using theMicroscope1. Always start off with
the lowest magnificationobjective and work upto higher power oneobjective at a time.
2. Always observe theobjective as it is rotatedinto place to ensure itdoesn’t crash into the slide.
Mechanical Stagemicroscope slide
Rules for Using theMicroscope1. Lower the stage to its
lowest point beforeplacing a slide on thestage.
2. Be sure the scanningobjective is in place.
3. Raise the stage to itshighest point withoutcrashing the slide intothe objective.
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Rules for Using theMicroscope4. Focus initially by
lowering the stageusing coarse focusadjustment knob.
5. After getting a closefocus with the coarsefocus adjustmentknob, improve it usingthe fine focusadjustment knob.
Substage Condenseriris
diaphragmlever
condenseradjustment
Rules for Putting Away theMicroscope1. Rotate scanning
objective into place.2. Lower the stage to its
lowest point.3. Clean lenses, stage,
body, and base ifneeded.
4. Replace protectivecover overmicroscope.
Lab ActivitiesGo to the Virtual Microscopewebsite and view theinstructional video online.
URL:http://www.udel.edu/Biology/ketcham/microscope/
Lab ActivitiesMake observations using thesimulated slides at the VirtualMicroscope website. Drawlabeled diagrams of vieweditems as described in theassignment.
Lab Activities
Complete the “try this” activities(see assignment for details).
Complete other tasks asdescribed in the assignment.