using the assay builder evolution, customer version)

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Using The Bio-Plex Assay Builder

How to find the Assay Builder:

www.bio-rad.com Select Life Science Research Catalog Index Drop-Down:

Select Multiplex Suspension Array System

Select Bio-Plex Express and x-Plex Assays Select Assay Builder

Or

www.bio-rad.com/bio-plex/x-plex Select Assay Builder

Page 1

Catalog # Naming Scheme:

Z Products ordered through the Assay Builder Expressoptions (e.g. Z50005KMTI).

M Products ordered through the Assay Builder x-Plexoptions (e.g. M5000B6AVC).

X Products ordered for rat cytokines, phosphoprotein, andtotal targets (e.g. X70IQQDQHR).

171- Products ordered for phosphoprotein and total targetswhen only one target and one plate is ordered

(e.g. 171-V27145).

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Using The Bio-Plex Assay Builder (step-by-step)

Catalog Number(Youre not done yet. Must continue to Step 8)

5. obtain pricing

Page 2

3. Make target selectionsNotes:1. For human and mouse cytokines, selecting

only one target will automatically direct theuser to the Express option.

2.For phospho and total targets, selecting onlyone target will automatically direct the user to

the Off-The-Shelf Singleplex assay option.

2. Select package size1. Select group

4. view catalog #

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6. Fill in contactinformation

7. I plan to orderI plan to order will ensure the catalog # is available the next day. Generally speaking, the catalog #

will be available next day if the submit button is pressed before 2pm.

Just looking will eventually generate the catalog # with considerable delay.

8. Submit

Page 3

Two Bullet Screen (Step 7) Express Phosphoprotein Phosphoprotein Total

Rat Cytokine

Four Bullet Screen (Step 7) Human Cytokine Group I (two to ten targets selected) Human Cytokine Group II (two to ten targets selected)

Mouse Cytokine Group I (two to ten targets selected) Mouse Cytokine Group II (two to ten targets selected)

Or

6. Contact

7. I plan to orderCustom blend (x-Plex, pre-mixed) or Express (non-mixed)

8. Submit

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Final Screen(no action needed on this screen)

10. Next day - Call Customer Service to place order.Refer to the catalog # on the email when placing the order next day. If the Assay Builder wascompleted late in the day (generally speaking >2pm), may need to wait another day. Youre finished!

See Pages 5, 6, and 7 for example emails.

9. Auto-generated email shortly after pressing submit.The email will arrive shortly after pressing submit.

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Anatomy of the auto-generated email(example from an Express or x-Plex order)

Catalog #For current orderx if x-Plex order

PriceFor current order

Shipping Info

ExpressPrice list for 1-10 targets whenExpress ordering

Optional ProductsOrdering info for extra

filter plates and standards

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Anatomy of the auto-generated email(example from a Rat order)

Catalog #For current order


Shipping Info

RequiredOrdering info for additional

products required to run anassay

Optional ProductsOrdering info for Diluent Kit

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Anatomy of the auto-generated email(example from a phosphoprotein or total targets order)

Catalog #For current order


Shipping Info

RequiredOrdering info for additional

products required to run anassay

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Whats Needed To Run An Assay

xxStandards

xxAssay Buffer

xxDiluent (Sample)

xxDiluent (Standard)

xxSealing Tape

xxFilter Plate

xxStreptavidin-PE

xxDiluent (det Ab)

xxWash Buffer

xxxDetection Ab

xxxBead (magnetic)

Included inSingleplex Sets

Included inAssay BuilderExpress*

Included inAssay Builderx-Plex*

Needed to runan assay

Bio-Plex Pro Cytokines (human, mouse)

* All-in-one kit formatBlack Components of the panels listed below (as well as x-Plex and Express

Panels and Singleplex Sets Assays):Human: 8-. 17-. 21-, 27-, Th1/Th2Mouse: 8-, 9-, 23-, Th1/Th2

Blue Components of Cytokine Reagent Kit (diluents included)

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xStandards

Wash Buffer

Assay Buffer

Diluent (Sample)

Diluent (Standard)

Sealing Tape

Filter Plate

Streptavidin-PE

Diluent (det Ab)

xDetection Ab

xBead (non-magnetic)


Needed to run anassay

Bio-Plex Cytokines (rat)

* Rat cytokines is not offered in ExpressBlack Components of the 9-Plex A and x-Plex Panels and Singleplex

Sets AssaysBlue Components of Cytokine Reagent KitGreen Components of Diluent Kit

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Resuspension Buffer

Wash Buffer

Assay Buffer

xxControls

Factor 2

Factor 1

Cell Wash Buffer

Cell Lysis Buffer

Sealing Tape

Filter Plate

Streptavidin-PE

Diluent (det Ab)

xxDetection Ab

xxBead (non-magnetic)

Included inSingleplex Assays



Bio-Plex Phosphoprotein

* Phosphoprotein is not offered in ExpressBlack Components of Phospho 3- and 5- Plex and x-Plex Panels andSingleplex Assays

Blue Components of Phosphoprotein Detection Reagent KitGreen Components of Cell Lysis Kit

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Resuspension Buffer

Wash Buffer

Assay Buffer

xxControls

Factor 2

Factor 1

Cell Wash Buffer

Cell Lysis Buffer

Sealing Tape

Filter Plate

Streptavidin-PE

Diluent (det Ab)

xxDetection Ab

xxBead (non-magnetic)




Bio-Plex Total Targets

* Total Targets are not offered in ExpressBlack Components of Total Targets 5-Plex and x-Plex Panels and Singleplex

AssaysBlue Components of Phosphoprotein Detection Reagent KitGreen Components of Cell Lysis Kit

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xxControls

xDiluent (det Ab)

xSealing Tape

xFilter Plate

xStreptavidin-PE

xWash Buffer

xAssay Buffer

xxStandards

xDiluent (standard)

xDiluent (sample)

xxDetection Ab

xxBead (magnetic)

Included inAssay Kits*

Included inComplete Kits*


Bio-Plex Pro Human Acute Phase

* Disease research assays are not ordered through the Assay BuilderBlack Components of 4- and 5-Plex PanelsBlue Components of Acute Phase Pro Reagent KitGreen Components of Acute Phase Diluent Kit

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xxControls

xWash Buffer

xAssay Buffer

xxStandards

xDiluent (standard)

xDiluent (sample)

xSealing Tape

xFilter Plate

xStreptavidin-PE

xDiluent (det Ab)

xxDetection Ab

xxBead (magnetic)

Included inAngiogenesis Panel*



Bio-Plex Pro Human Angiogenesis

* Disease research assays are not ordered through the Assay BuilderBlack Components of 9-Plex PanelBlue Components of Pro Angiogenesis Reagent KitGreen Components of Human Serum Diluent Kit

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xxxxControls

x

x

x

Included in

(2-Plex Panel)

x

x

x

Included in(12-Plex Panel)

x

x

x


(Adiponectin, Adipsin)

xWash Buffer

xAssay Buffer

xStandards

xDiluent (serum)

xDiluent (serum-free)

xSealing Tape

xFilter Plate

xStreptavidin-PE

xDiluent (det Ab)

xDetection Ab

xBead (magnetic)

Included inComplete Kits

(12- + 2-Plex)


Bio-Plex Pro Human Diabetes

* Disease research assays are not ordered through the Assay BuilderBlack Components of 12- and 2-Plex Panels and Singleplex AssaysBlue Components of Pro Diabetes Reagent KitGreen Components of Pro Diabetes Diluent Kit

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xxControls

xDiluent (det Ab)

xWash Buffer

xAssay Buffer

xxStandards

xDiluent (serum)

xSealing Tape

xFilter Plate

xStreptavidin-PE

xxDetection Ab

xxBead (magnetic)

Included in

7-Plex Panel*



Bio-Plex Pro Human Isotyping

* Disease research assays are not ordered through the Assay BuilderBlack Components of 7-Plex PanelBlue Components of Pro Isotyping Reagent KitGreen Components of Pro Isotyping Diluent Kit

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xWash Buffer

xAssay BufferxControls

xStandards

xDiluent (plasmastandard)

xDiluent (serumstandard)

xDiluent (sample)

xSealing Tape

xFilter Plate

xStreptavidin-PE

xDiluent (det Ab)

xDetection Ab

xBead (magnetic)

Included inAll-In-One*


Bio-Plex Precision Pro Human Cytokine 10-Plex

* Precision Pro is not ordered through the Assay BuilderBlack All components included in all-in-one kit format

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using the assay builder evolution, customer version)

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