updated list of experiment for biochem lab sy 2012-2013

7/30/2019 Updated List of Experiment for BIOCHEM LAB SY 2012-2013

1/4

Experimen

t no.

Title ExpectedDate to

perform

the

experimen

t

Reagent Equipment

( Per group)

6 groups

To be

provided by

the

students

4 COLLOID AND

THEIR

PROPERTIES

January

12, 2013

0.1 M CuSO4,

10% skim milk

mixture, 3 M

HAc, 0.2

K2Cr2O7, 0.04 M

AgNO3, 0.02 M

K2Cr2O7 1%

gelatin, 0.01 M

AgNO3, dialysis

bag

9 test tubes Longanisa

skin,

distilled

water,

gelatin

powder

5 LIPID January19, 2013

KHSO4, glycerol,coconut oil,

stearic acid, olive

oil, oleic acid,

stearic acid, Br2,

lecithin, 5%

(NH4)2 MoO4 (

freshly

prepared), conc.

HNO3, 1% bile

salt solution, 1%

lecithin solution,

cholesterol,

dichloromethane

, acetic

anhydride, conc.

H2SO4, 0.05% -

tocopherol in

CHCl3, n-butyl

alcohol

TEST TUBE(50,BEAKER (1),

BUNSEN

BURNER,

PORCELAIN

CRUCIBLE,

THERMOMETE

R, TEST TUBE

RACK, HOT

PLATE,

SPATULA,

DROPPERS,

FILTER PAPER,

TRIPOD,

TONGS,

FUNNEL, 10 ml

pipette

DISTILLEDWATER (1

L)

6 ANALYSIS AND

DENATURATION

OF PROTEINS

January

26

5% ALBUMIN, 5%

GELATIN, CONC.

HNO3, conc.

NH4OH, 5%phenol, tyrosine,

Millons reagent

conc. H2SO4,

10% NaOH, 5%

lead acetate

solution,

peptone, 0.1 %

TEST TUBES

(15), TEST TUBE

HOLDER, TEST

TUBE BRUSH,DROPPER,

PIPETTE,

ASPIRATOR,

SPATULA,

STIRRING ROD,

BUNSEN

BURNER,

albumin (

from fresh

egg)


2/4

ninhydrin

reagent,

ammonia water,

0.2% urea, 95%

ethanol, 2%muriatic

chloride, 2%

silver nitrate,

picric acid, tannic

acid conc. HCl,

phenylalanine

TRIPOD, WIRE

GAUZE, WATCH

GLASS, WATER

BATH,

GRADUATEDCYLINDER,

CORK, egg

7 PROTEIN &

AMINO ACID

February

2

1% albumin, 0.1%

valine, unknown,

10% NaOH, 0.1 %

CuSO4, 0.1%

ninhydrin, 0.1%

tyrosine, 0.1 %

tryptophan, conc.

HNO3, 20%

NaOH, Millon-

Nasse, 0.1%

NaNO3, glyoxalic

acid, conc.

H2SO4,0.1 %

arginine, dilute -

naphthol

solution, alcohol,

NaOBr, 0.1 %cysteine, 10%

albumin, 5 M

HOAc, ethanol,

picric acid, TCA,

2% CuSO4, 2%

FeCl3, 10%

Pb(OAc)2,

skimmed milk,

0.05 M HCl, 1 M

HCl, 1 M NaOH,

(NH4)2SO4(s),NaCl(s), n-

butanol:HOAc:H2O ( 100:22:50),

0.1 % ninhydrin

SMALL TEST

TUBE(20),

BUNSEN

BURNER, pH

meter, filter

paper ( 5 X 4),

test tube rack,

250 ml beaker,

hot plate,

marble, 50 ml

beaker, stirring

rod, spatula,

TLC plate

DISTILLE

WATER,

SKIMMED

MILK,

RULER,

PENCIL,

hair,

8 ENZYME

DIGESTION

February

9

.10 ml HCl, 2%

pepsin, 2% starch

solution, KI

TEST TUBE (7),

THERMOMETER

, BEAKER,

BOLIED EGG

( WHITE

ONLY),


3/4

solution, 0.5 %

Na2CO3, 0.2%

HCl, Fehlings A

and B

BUNSEN

BURNER,10 ml

pipette, 50-ml

beaker, hot

plate

DISTILLED

WATER

9 ENZYMES AND

FACTORS THAT

AFFECT ENZYME

ACTIVITY

Februar

y 16

6% H2O2guaiac

solution, starch

solution,

acidified iodine

solution, 0.1 M

NaCl, 0.05 N HCl,

0.05 N NaOH,

Blender, funnel,

wooden splint ,

test tube (10), ,

filter paper or

cotton/gauze,

pH meter,

Bunsen burner,

thermometer,

pH paper,

1 PC.

SMALL

POTATO

WITH PEEL,

saliva,

cheesecloth

, distilled

water

10 ISOLATION AND

CHARATERIZATIO

N OF DNA

Feb. 23 0.10 M

phosphate buffer

( pH=7), 1% SDS (

sodium dodecyl

sulfate), 1.0 M

NaCl, chloroform:

isoamyl alcohol (

24:1), cold

ethanol, 10%

NaOH, 0.5%

CuSO4, 10%

H2SO4, 10%

NH4OH, 10%

HNO3,hydrolyzate,

AgNO3 solution,

Bials orcinol

reagent, 0.1%

ribose, 0.1 %

glucose,

diphenylamine,

5% (NH4)2MoO4

10 ml pipette,

centrifuge,hot

water bath, ice

bath, Pasteur

pipette, test

tube holder,

stirring rod,

glass rod,

dropper

THERMOMETER

, ICE,

WEIGHING

SCALE,

GRADUATEDCYLINDER, pH

meter,

MORTAR AND

PESTLE, SMALL

TEST TUBE (3),

BIG TEST TUBE

(3)

CHICKEN

LIVER

11 NUCLEIC ACID MARCH

2

5% ammonia

molybdate, 0.1%

ribose solution,0.1 % glucose,

Bial ornicol

reagent, 10%

NH4OH, 5%

AgNO3, yeast,

white sand, 0.2%

NaOH, 1% CuSO4,

MORTAR AND

PESTLE, 4

BEAKERS ( 250mL), graduated

cylinder, pipet,

aspirator,

watch glass,

tripod, Bunsen

burner, test

tubes (8), test

Cheesecloth

,


4/4

10% H2SO4, 10%

HNO3

tube rack ,

filter paper

12 ANALYSIS OF

URINE

MARCH

9

5% sucrose,

concentratedH2SO4,

pathological

urine sample (

containing 5%

glucose, 5%

albumin,

acetone, bile,

blood solution)

Concentrated

NaOH, bromine

water,

concentrated

HCl, picric acid,

10% NaOH, (NH4)

2SO4-NH3

Reagent, 3%

H2O2, benzidine

in glacial HAc,

sodium

nitroprusside,

Benedicts

reagent,

concentratedHNO3, 2% acetic

acid

Pipette,

aspirator, testtube (10), test

tube holder,

test tube rack,

graduated

cylinder,

beaker 250 mL,

stirring rod,

litmus paper,

Bunsen burner,

tripod, water

bath, pH paper

If there are classes beyond this date, well let you know for additional listing of materials,

equipment and reagents.

updated list of experiment for biochem lab sy 2012-2013

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