UNIVERSITY OF SOUTH FLORIDA

Division of Allergy and Immunology

Department of Internal Medicine

Joy McCann Culverhouse Airway Disease Research Center

and The James A. Haley V.A. Medical Center

Tampa, Florida

13000 Bruce B. Downs Blvd., (111D)Tampa, Florida 33612

Phone: (813) 972-7631Fax: (813) 910-4010

Annual Report

http://health.usf.edu/medicine/internalmedicine/allergy/index.htm

2012 - 2013

Contents

I. Greetings

II. Faculty, Staff and Fellows-in-Training

III. Joy McCann Culverhouse Airway Disease Research Center

A. Basic Research ProjectsB. Clinical Research ProjectsC. Clinical Research Unit

IV. Basic and Clinical Research Support

V. Publications

VI. Faculty and Staff Awards

VII. Visiting Professor Educational Program

I. GREETINGS!

The late Samuel C. Bukantz, MD, founded the University of South Florida College of Medicine, Department of Internal Medicine, Division of Allergy and Immunology in 1972. Richard F. Lockey, M.D. succeeded Dr. Bukantz in 1983 and is the current Director of the Division. Mrs. Joy McCann Culverhouse endowed the Division in 1997 and The Joy McCann Culverhouse Airway Disease Research Center was dedicated in February 1998. In 1998, Mabel and Ellsworth Simmons endowed the Division with a grant for education and research.

The goals of the Division are: first, to provide care to patients with allergic and immunologic diseases at the University of South Florida College of Medicine, Tampa General Hospital, James A. Haley V.A. Medical Center, All Children’s Hospital, and H. Lee Moffitt Cancer Center; second, to train students, residents, and fellows in the subspecialty of allergy and immunology; and third, to conduct basic and clinical research in allergy, asthma, and immunology.

Individuals interested in collaborating with members of the Division may contact Richard F. Lockey, M.D. or any faculty member at (813) 972-.7631 (email: rlockey@health.usf.edu).

Mark Ballow, M.D., Professor, Department of Pediatrics and Internal Medicine, Division of Allergy & Immunology, may be contacted at (727)-553-3533 (email: mballow@health.usf.edu)

Richard F. Lockey, MDDistinguished University Health ProfessorProfessor of Medicine, Pediatrics & Public HealthJoy McCann Culverhouse Chair of Allergy and ImmunologyDirector, Division of Allergy and ImmunologyUniversity of South Florida Morsani College of MedicineDepartment of Internal MedicineandChief, Section of Allergy/ImmunologyJames A. Haley Veterans’ HospitalTampa, Florida

THE UNIVERSITY OF SOUTH FLORIDA IS AN AFFIRMATIVE ACTION EQUAL OPPORTUNITY INSTITUTION

MORSANI COLLEGE OF MEDICINEUNIVERSITY OF SOUTH FLORIDA

DIVISION OF ALLERGY AND CLINICAL IMMUNOLOGY (A/I)DEPARTMENT OF INTERNAL MEDICINE

V.A. MEDICAL CENTER13000 BRUCE B. DOWNS BOULEVARD (111D)

TAMPA, FLORIDA 33612(813) 972-7631

FAX: (813) 910-4041

FACULTY

RICHARD F. LOCKEY, M.D.Professor; Medicine, Pediatrics & Public HealthDivision Director, Internal Medicine A/IJoy McCann Culverhouse Chair

JOHN W. SLEASMAN, M.D.Professor; Pediatrics & MedicineChief, Pediatric A/I & Rheumatology

NOORBIBI K. DAY-GOOD, Ph.D.Professor Emer; Pediatrics, Medicine & Public Health

ROGER W. FOX, M.D.Professor; Medicine, Pediatrics & Public Health

DENNIS K. LEDFORD, M.D.Professor; Medicine & PediatricsMabel & Ellsworth Simmons Professor

GARY LITMAN, Ph.D.Professor; Pediatrics & Medicine

MARK C. GLAUM, M.D., Ph.D.Associate Professor; Medicine & Pediatrics

MITCHEL J. SELEZNICK, M.D.Associate Professor; Medicine

SANDRA G. GOMPF, M.D.Associate Professor; Medicine

NARASAIAH KOLLIPUTI, Ph.D.Associate Professor; Medicine & Pediatrics

MICHAEL TENG, Ph.D.Associate Professor; Medicine & Pediatrics

MARK BALLOW, M.D. Professor; Pediatrics & Medicine

PANIDA SRIAROON, M.D.Assistant Professor; Pediatrics & Medicine

JENNIFER LEIDING, M.D.Assistant Professor; Pediatrics & Medicine

JIA-WANG WANG, Ph.D.Assistant Professor; Medicine & Pediatrics

GLENN WHELAN, Pharm.D.Clinical Assistant Professor; Medicine

ENRIQUE FERNANDEZ-CALDAS, Ph.D.Clinical Professor; Medicine

MANDEL SHER, M.D.Professor; Pediatrics & Medicine

NATHAN TANG, M.D.Associate Professor; Pediatrics & Medicine

MONROE J. KING, D.O.Adjunct Clinical Associate Professor; Medicine

BRETT E. STANALAND, M.D.Clinical Associate Professor; Medicine

G. EDWARD STEWART II, M.D.Clinical Associate Professor; Medicine

HUGH H. WINDOM, M.D.Clinical Associate Professor; Medicine

BLANCA CAMARETTI-MERCADO, Ph.D.Ass’t Professor; Personalized Med & Medicine

ROSA CODINA, Ph.D.Clinical Assistant Professor; Medicine

MARY L. JELKS, M.D.Clinical Assistant Professor; Medicine

RONALD T. PURCELL, M.D.Clinical Assistant Professor; Medicine

2

3

II. FACULTY AND STAFF

Core Faculty

* Richard F. Lockey, M.D., University Distinguished Health Professor; Professor of Medicine, Pediatrics, and Public Health; Division Director; Joy McCann Culverhouse Chair of Allergy and Immunology

Thomas B. Casale, M.D., Professor of Medicine

* Roger W. Fox, M.D., Professor of Medicine, Pediatrics and Public Health

* Mark C. Glaum, M.D., Ph.D., Associate Professor of Medicine and Pediatrics

* Dennis K. Ledford, M.D., Professor of Medicine and Pediatrics; Mabel & Ellsworth Simmons Professor

Narasaiah Kolliputi, Ph.D., Associate Professor of Medicine & Pediatrics

Michael Teng, Ph.D., Associate Professor of Medicine & Pediatrics

Jia-Wang Wang, Ph.D., Assistant Professor of Medicine & Pediatrics

Joint Faculty

Mark Ballow, M.D., Professor of Pediatrics and Internal Medicine; Director, Pediatric Allergy & Immunology

Blanca Camoretti-Mercado, Ph.D., Assistant Professor of Personalized Medicine and Medicine

* Sandra G. Gompf, M.D., Associate Professor of Medicine

Jennifer Leiding, M.D., Assistant Professor of Pediatrics and Medicine

Gary W. Litman, Ph.D., Professor of Pediatrics and Medicine; University Distinguished Health Professor; Andrew and Ann Hines Chair in Pediatrics

Mandel R. Sher, M.D., Clinical Professor of Pediatrics and Medicine

Panida Sriaroon, M.D., Assistant Professor of Pediatrics and Medicine

Nathan Tang, M.D., Associate Professor of Pediatrics and Medicine

Clinical Faculty

Enrique Fernandez-Caldas, Ph.D., Clinical Professor of Medicine

* Monroe J. King, D.O., Adjunct Clinical Associate Professor of Medicine

* Brett E. Stanaland, M.D., Clinical Associate Professor of Medicine

* G. Edward Stewart II, M.D., Clinical Associate Professor of Medicine

* Hugh H. Windom, M.D., Clinical Associate Professor of Medicine

Rosa Codina, Ph.D., Clinical Assistant Professor of Medicine

Mary L. Jelks, M.D., Clinical Assistant Professor of Medicine

* Ronald T. Purcell, M.D., Clinical Assistant Professor of Medicine

Glenn Whelan, Pharm D, Clinical Assistant Professor of Medicine* Have joint appointments in the Section of Allergy and Immunology, Department of Internal Medicine, James A. Haley

Veterans’ Hospital, Tampa, Florida

4

Richard F. Lockey, M.D., M.S.

Dr. Richard F. Lockey received his B.S. degree from Haverford College, Haverford, Pennsylvania; M.D. from Temple University, Philadelphia, Pennsylvania (Alpha Omega Alpha); M.S. from the University of Michigan in Ann Arbor, Michigan where he trained in Internal Medicine and Allergy/Immunology (A/I) and was a Major and Chief of A/I at Carswell Air Force Base, Fort Worth, Texas, from 1970-1972. He received a medal from the Florida Academy of Sciences, Tallahassee, Florida, in 2000, for his dedication and work to improve the health and well-being of the community and citizens of Florida. He was also the recipient of the Southern Medical Society, Dr. Robert D. and Alma W. Moreton Orginal Research Award in 2012. The American Academy of Allergy Asthma and Immunology presented him with a Special Recognition Award in 1993, Distinguished Service Award in 1999, and Distinguished Clinician Award in 2008. He has the honor of authoring, co-authoring or editing over 600 publications and 35 books or monographs with colleagues and has lectured on numerous occasions nationally and internationally. He is the co-editor of two books and an encyclopedia of allergy/immunology, with Dennis K. Ledford, MD, published in 2014: Asthma, Comorbidities, Co-Existing Conditions, and Differential Diagnoses, Oxford University Press; Allergens and Allergen Immunotherapy: Subcutaneous, Sublingual and Oral, 5th edition, CRC Press/Taylor & Francis Group; and Encyclopedia of Infection and Immunity, Springer, Inc. Professional honors include President of the American Academy of Allergy Asthma and Immunology (1992), past Director of the American Board of Allergy and Immunology (1993-1998) and President of the World Allergy Organization (2010-2012). He has served as co-editor or participant of two WHO reports and served on many journal editorial boards.

Over 90 physician specialists and 50 international post-graduate PhDs or MDs in basic and clinical research and medicine, many of whom have assumed leadership positions in medicine throughout the world, have been trained in the Division. The Division’s staff consists of 5 clinicians, 3 basic scientists, and approximately 60 other healthcare professionals including physicians, support, and laboratory personnel.

Areas of expertise and research: insect allergy; allergen immunotherapy; asthma; inflammatory lung diseases; pulmonary fibrosis; co-morbid conditions of asthma; and respiratory syncytial virus vaccine development.

5

Thomas B. Casale, M.D.

Before joining USF in October 2013, as Professor of Medicine and Chief of Clinical and Translational Research, Dr. Thomas Casale was Professor of Medicine and Medical Microbiology and Immunology and Chief of Allergy/Immunology at Creighton University, Omaha, Nebraska. He did an allergy/immunology fellowship at the National Institutes of Health, Bethesda, MD, where he was chief medical staff fellow. From 1984 to 1996 he was at the University of Iowa where he attained the rank of Professor of Medicine and Director of Allergy/Immunology.

Dr. Casale is a member of the American Thoracic Society and served on their Board of Directors; American Society for Clinical Investigation; and a Fellow of the American College of Physicians and both the American College and American Academy of Allergy Asthma and Immunology. He is a Past President of the American Academy of Allergy Asthma and Immunology and the current Executive Vice President. He is a past member of the Board of Directors of the World Allergy Organization. He also served on the American Board of Allergy and Immunology and was Chair from 2005-2006.

Dr. Casale’s clinical and basic research interests are directed toward the determination and treatment of the pathophysiologic mechanisms involved in asthma and allergic diseases. He has published over 300 scientific papers, reviews and chapters on these topics.

6

Roger W. Fox, M.D.

After receiving his medical degree from St. Louis University School of Medicine, Dr. Roger W. Fox completed his 3 years of internal medicine and 2 years in allergy and immunology at the University of South Florida, Morsani College of Medicine, Tampa, FL. He joined the Division’s faculty in July, 1980. He is a Fellow of the American College of Physicians and the American Academy of Allergy Asthma and Immunology.

He has been elected to “The Best Doctors in America” for the past decade. He serves as the Director of Education of the allergy/immunology fellowship training program and has helped train over 75 physicians in this specialty. He has published extensively and presented at local, national and international medical meetings and has served on various boards, including the Hillsborough County Medical Association, the Florida Allergy Asthma and Immunology Society of which he has been president, and numerous committees in the American Academy of Allergy Asthma and Immunology.

Dr. Fox enjoys being a clinician and mentor and in that capacity, sees patients at the University of South Florida Morsani Medical Clinics, the Veterans’ Administration Hospital Allergy Clinic, as well as the other clinics affiliated with the University of South Florida. Dr. Fox is an attending physician at the James A. Haley Tampa VA Hospital and he has staff privileges at Tampa Genera Hospital, Moffitt Cancer Hospital, and Florida Hospital.

His research interests include vocal cord dysfunction, urticaria and angioedema, comorbid conditions of asthma, allergic drug reactions and atopic eczema.

7

Mark C. Glaum, M.D., Ph.D.

Dr. Mark Glaum was born and raised in the Philadelphia area of southeastern Pennsylvania. He earned his B.A. in psychology from Fordham University, New York City. Following graduation, he returned to Philadelphia, PA, and enrolled in the Graduate School at Hahnemann University where he was awarded a Master of Science in Physiology. Immediately following graduate school, he enrolled in Hahnemann Medical School, Philadelphia, PA, and was accepted into their combined MD/PhD program, where he was awarded an MD degree as well as a PhD in immunology. He stayed at Hahnemann Hospital to complete his internship and residency in internal medicine and then completed a fellowship in allergy and immunology at the University of Pennsylvania, Philadelphia, PA, where he received the Stanley E. Bradley Award for Bench Research in Internal Medicine. Dr. Glaum is board certified in both internal medicine and allergy and immunology and is a fellow of the American Academy of Allergy Asthma and Immunology.

Dr. Glaum is an associate professor of medicine and pediatrics at the Morsani College of Medicine, University of South Florida. As a faculty member in the Division of Allergy and Immunology at the College of Medicine, Dr. Glaum is interested in research and education with teaching responsibilities for medical students, residents and fellows who are training to become allergy and immunology specialists.

In addition to teaching, Dr. Glaum’s research interests include mast cell biology, the study of chronic sinusitis with nasal polyps and pollen identification using novel molecular techniques.

8

Dennis K. Ledford, M.D.

Dr. Dennis Ledford received his medical degree from the University of Tennessee Center for Health Sciences, Nashville, Tennessee. He completed his internal medicine residency there and served as chief medical resident for Dr. Gene Stollerman, M.D., Chairman of Internal Medicine at this same university. A fellowship in rheumatology and immunology followed at New York University and Bellevue Hospital in New York as well as a fellowship in allergy and immunology at the University of South Florida. He joined the faculty at USF Morsani College of Medicine and achieved the rank of professor of medicine in 2000.

Local and regional activities include past service as President of the USF Medical Faculty for the Morsani College of Medicine and President of the Florida Allergy Asthma and Immunology Society. He is current Head of the Allergy/Immunology Section, Florida Hospital,Tampa, FL. National contributions include current service as an associate editor of the Journal of Allergy and Clinical Immunology and chair of the Steering Committee for the Allergy Asthma and Immunology Education and Research Trust Fund. He also served as President of the American Academy of Allergy Asthma and Immunology, Co-Chair of the American Council of Graduate Medical Education (ACGME), Allergy/Immunology Residency Review Committee and Director of the American Board of Allergy and Immunology.

Clinical responsibilities and student and resident teaching are combined with research interests in severe, glucocorticoid-dependent asthma, allergen characterization, the association of gastroesophageal reflux and upper airway disease, and eosinophilic esophagitis.

9

Narasaiah Kolliputi, Ph.D.Dr. Narasaiah Kolliputi is an associate professor (tenured) and Division Director of Research Education for the Division of Allergy and Immunology, Department of Internal Medicine, at the USF Morsani College of Medicine, Tampa, FL. He received his postdoctoral training at Massachusetts General Hospital, Harvard Medical School, Boston, MA. Prior to that time, he received his BS in biology and chemistry in 1997 followed by an MS in biochemistry in 1999 at Sri Venkateswara University, Tirupati, India. He then went on to do his PhD in biochemistry at Osmania University, Hyderabad, India, which he completed in 2004.

Dr. Kolliputi has published 25 papers, including a paper in Circulation Research and Immunology. He currently serves as a grant reviewer for the National Institutes of Health, VA Merit Grants, USA Department of Defense and the American Heart Association. He is an associate editor for Frontiers in Pharmacology, Frontiers in Physiology, and Frontiers in Genetics, and is a guest associate editor for Frontiers in Mitochondrial Physiology. He is also an editorial board member for Translational Medicine, Virology & Mycology and Journal of Biocatalysis & Biotransformation. Dr. Kolliputi’s research is funded by an NIH RO1 and an American Heart Association Scientist Developmental grant.

He is working on translational strategies to attenuate oxidative stress mediated acute lung injury (ALI), pulmonary fibrosis and pulmonary arterial hypertension.

10

Michael Teng, Ph.D.

Dr. Michael Teng received his Ph.D. in immunology from the University of Chicago in 1993. He trained as a postdoctoral fellow studying viral pathogenesis at The Scripps Research Institute in La Jolla, CA. Subsequently, he became a research fellow at the National Institute of Allergy and Infectious Diseases, investigating the molecular biology of respiratory syncytial virus (RSV) and RSV vaccine development. In 2002, he accepted a faculty appointment in the Department of Biochemistry and Molecular Biology at the Pennsylvania State University, University Park, PA, where his laboratory continued studies on RSV pathogenesis.

Dr. Teng joined the faculty of the Division of Allergy and Immunology at USF Morsani College of Medicine in 2010 and is director of the basic research program in the Division. He holds joint appointments in the Departments of Pediatrics, Molecular Medicine, and Pharmaceutical Sciences (College of Pharmacy). Dr. Teng currently serves as a grant reviewer for the National Institutes of Health and the American Heart Association. His past and present research funding includes grants from the National Institutes of Health, the American Heart Association, and contracts with pharmaceutical companies.

Dr. Teng’s research focuses on the host-virus interactions important for RSV pathogenesis. In particular, he is interested in the mechanisms by which RSV inhibits innate immune responses to enhance viral replication. Understanding the interplay between RSV proteins and innate immunity may lead to the development of more immunogenic RSV vaccine candidates. Additionally, Dr. Teng studies the interactions between cellular signal transduction machinery and viral proteins, with a view to discovering potential targets for antiviral therapy.

11

Jia-Wang Wang, Ph.D.

Dr. Jia-Wang Wang received an M.S. degree from Sichuan University, Chengdu, China and a Ph.D. degree from Wuhan University, Wuhan, China. He conducted postdoctoral research at the Chinese Academy of Medical Sciences, Beijing, China and at the Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA. He then worked as a research associate at the H. Lee Moffitt Cancer Center & Research Institute, Tampa, FL, before he joined the Division of Allergy and Immunology. Dr. Wang is a member of the American Academy of Allergy, Asthma & Immunology (AAAAI) and an editorial board member of the Austin Journal of Clinical Immunology.

He has a strong background in genetics, immunology and molecular biology, discovered the lipopolysaccharide-responsive beige-like anchor (LRBA) gene, mutation of which causes immunodeficiency and autoimmunity, and contributed fifteen GenBank sequence entries. He has published more than 24 papers, some of which are in high profile journals including Science. He has more than fourteen conference abstracts including featured posters and oral presentations. He has three approved patents, one copyright computer program and three patents pending. Most of these intellectual properties are on microRNAs (miRNA), which have great promise as biomarkers and therapeutics for human diseases.

He also has extensive experience working with mouse models to study human diseases. He has successfully generated SHIP gene null and conditional knockout mouse models, two LRBA knockout mouse models, and four miRNA transgenic overexpression mouse models.

12

DIVISION OF ALLERGY AND IMMUNOLOGY

FELLOWS-IN-TRAINING (2012 to 2015)

Adam Updegraff, DO, 1st year fellow, is scheduled to graduate in June, 2015. Dr. Updegraff received his medical degree from Michigan State University College of Osteopathic Medicine in East Lansing, Michigan. He completed a combined residency in internal medicine and pediatrics from William Beaumont Hospital, Royal Oak, Michigan. Dr. Updegraff’s research interestsinclude asthma, atopic dermatitis, and food allergy.

Michael Balduzzi, MD, 1st year fellow, is scheduled to graduate in June 2015. Dr. Balduzzi received his medical degree from New York Medical College, Valhalla, NY. He completed his residency in internal medicine at the University of South Florida Morsani College of Medicine,Tampa, FL where he also spent time in his final year of residency as one of the program’s chief resident. Dr. Balduzzi has research interest in local mucosal factors playing a role in chronic allergic and nonallergic rhinitis and improving asthma outcomes by controlling upper airway comorbid conditions.

Gregory Cowan, MD, 2nd year fellow, is scheduled to graduate in June, 2014. Dr. Cowan received his medical degree from the University of South Florida Morsani College of Medicine, Tampa, Florida. He completed his residency in pediatrics at Phoenix Children’s Hospital/Maricopa Medical Center, Phoenix, Arizona. His research interest in allergy & immunology involves primary immune deficiencies and asthma in children. His long term goal is to improve patient and parent understanding of how to manage asthma and improve long-term outcomes.

Nauman Salim, MD, 2nd year and chief fellow, is scheduled to graduate in June, 2014. Dr. Salim received his medical degree from Allama Iqbal Medical College, Lahore, Pakistan. He completed his residency in internal medicine and pediatrics from Indiana University School of Medicine, Indianapolis, Indiana. His research interest includes blood and bone marrow transplantation with emphasis on graft versus host disease. His long term goal is to practice allergy and immunology and provide immunologic expertise for patients undergoing stem cell transplantation.

From left to right: Adam Updegraff, DO, Michael Balduzzi, MD, Nauman Salim, MD, Gregory Cowan, MD

13

RESEARCH STAFF MEMBERS

Lakshmi Galam, Ph.D., Research Instructor

Jutaro Fukumoto, M.D., Ph.D., Postdoctoral Fellow

Kunyu Li, Biological Scientist

Kim Teng, Senior Biological Scientist

Michelle Reiser, M.S., Research Technician

Eileen Rifkin, Research Technician

STUDENTS AND VISITING RESEARCH SCHOLARS

Ruan Cox, Graduate Student

Sara Garcia, Research Student

Matthew Ho, Research Student

Amanda Hodgkins, Research Assistant/Student

Bao Huynh, Research Assistant/Student

Michelle Kaminsky, Research Assistant/Student

Tucker Maute, Research Student

Anu Stephen, Research Student

Tran Tran, Graduate Student

Jillian Whelan, Graduate Student

Olivia Smith, Volunteer Research Student

Bangmei Wang, Volunteer Research Student

Rebekah Cook, Volunteer Research Student

ADMINISTRATIVE PERSONNEL

* Peggy Hales, Program Assistant

* Rebecca Carter, Administrative Secretary

* Geeta Gehi, Administrative Secretary

* Also James A. Haley Veterans’ Hospital, Tampa, FL

14

CLINICAL RESEARCH UNIT

Carlotta Riner, CRC, Clinical Research Administrator

Shawna Ogilbee, CRC, Unit Manager

Jeaneen Ahmad, BA, ALA Coordinator

Sarah Croker, RN, CRC, BA, Clinical Research Coordinator

Rebecca McCrery, AS, Regulatory Specialist, Coordinator

ALL CHILDREN’S HOSPITAL

Amy Baldwin, Administrative Assistant to Dr. Mark Ballow

Marjorie Peak, Pediatric Allergy and Immunology Fellowship Coordinator

15

III. JOY MCCANN CULVERHOUSE AIRWAY DISEASE RESEARCH CENTER

A. Basic Research Projects1. Akt as a therapeutic target for respiratory syncytial virus (PI:

Teng)

Two aspects of Akt activity in RSV infection are being explored. First, RSV phosphoprotein has been identified as a target for Akt phosphorylation and the role of this phosphorylation on viral replication is under investigation. Second, the process of understanding the viral factors responsible for Akt activation during RSV infection is being researched. These studies are being done in collaboration with Dr. Biao He (University of Georgia, Athens, Georgia).

2. Structural determinants of NS2 for pathogenic functions (PI: Teng)

We have previously published that NS2 blocks interferon induction by binding to RIG-I. In addition NS2 appears to have additional functions associated with viral pathogenesis, including NFkB induction and STAT2 degradation. Trying to separate these activities by mutagenesis to understand how NS2 accomplishes each function is under investigation. The focus is on differentially altering the functions to develop an attenuated RSV vaccine candidate that maintains its immunogenicity.

3. Enhancing immunogenicity of RSV vaccines by altering NS1 function (PI: Teng)

This is part of a program project in collaboration with Dr. Mark Peeples (Nationwide Children’s Hospital, Columbus, OH). The hypothesis that decreasing the ability of NS1 to inhibit interferon responses can enhance the immunogenicity of RSV vaccine candidates is being researched.

4. Mechanism of RSV temperature sensitivity due to cis-acting sequences (PI: Teng)

Previous studies show that a single nucleotide change in the M2 transcription start sequence is sufficient to confer temperature sensitivity to recombinant RSV. The mechanism is being investigated by which this mutation affects RSV replication and transcription at non-permissive temperatures.

5. RSV M protein trafficking and virus assembly (PI: Teng)

This is a long-term collaboration with Drs. David Jans and Reena Ghildyal (Monash University, Melbourne, AUS) to determine the role of M protein trafficking in RSV morphogenesis and the importance of nuclear translocation in M function.

16

6. Effect of NALP-3 inflammasome on epithelial permeability (PI: Kolliputi)

Our previous reports demonstrate the inflammasome, a poinflammatory cytokine processing complex, plays an important role in the production of early inflammatory cytokines associated with edema. Ceramide is a critical mediator of pulmonary edema, however the ability of ceramide to activate the inflammasome has not been elucidated. Utilizing macrophages in vitro, we discovered that ceramide induced inflammasome activation results in significant cytokine secretion. Genetic silencing of inflammasome components abolished the ability of ceramide to induce inflammasome activation, and a rescue of the barrier integrity of alveolar epithelial cell (AEC) in co-culture was observed. These novel results reveal that ceramide induced cytokine secretion and AEC permeability occurs through an inflammasome dependant mechanism

7. Effect of microRNA 16 on epithelial sodium channel in human alveolar epithelial cells (PI: Kolliputi)

Removal of edema from the air spaces of the lung is a critical function of the epithelial sodium channel (ENaC) and also involves the serotonin (5HT) transport system. Recent studies suggest that microRNA-16 (miR-16) targets the serotonin transporter (SERT). However, the role of miR-16 on its targets SERT and ENaC have not been studied. The expression patterns of miR-16, SERT, ENaC and serotonin are being investigated in mice exposed to room air and hyperoxia. The effects of miR-16 overexpression are being observed in vitro. MiR-16 and ENaC down regulation in mice exposed to hyperoxia correlates with an increase in SERT expression and pulmonary edema. Overexpression of miR-16 in alveolar epithelial cells suppresses SERT and increases ENaCβ levels. These data suggest that miR-16 upregulates ENaC, a major sodium channel involved in resolution of pulmonary edema in acute lung injury (ALI).

8. Role of enhancer of zeste homolog 2 on pulmonary artery smooth muscle cell proliferation (PI: Kolliputi)

Pulmonary arterial hypertension (PAH) is characterized by excessive proliferation of the pulmonary arterial smooth muscle cells (PASMCs). EZH2 regulates cancer cell proliferation; however, the role of EZH2 in the proliferation of PASMCs is not clear. Therefore, the expression of EZH2 is being investigated in normal and hypertensive mouse PASMCs. The effects of EZH2 overexpression on the proliferation of human PASMCs also are being tested. EZH2 protein expression in mouse PASMCs correlates with decreased right ventricular function. The overexpression of EZH2 in human PASMCs enhances proliferation and migration and decreases the rate of apoptosis. EZH2 transfected cells demonstrated an increase in proliferation and a significant decrease in apoptosis. These findings show that EZH2 plays a role in the migration and proliferation of PASMCs. More importantly, EZH2 may serve as a potential target for new therapies for PAH.

17

9. Effect of mir-206 on pulmonary artery smooth muscle cell proliferation and differentiation (PI: Kolliputi)

Pulmonary arterial hypertension (PAH) is a progressive devastating disease characterized by excessive proliferation of the pulmonary arterial smooth muscle cells (PASMCs). MicroRNA-206 (miR-206) is known to regulate proliferation however, the role of miR-206 in PAH has not been studied. Therefore, the expression patterns of miR-206 are being investigated in normal and hypertensive mouse PASMCs. The effects of miR-206 on cell proliferation, apoptosis and smooth muscle cell marker expression in human PASMCs also are being measured. MiR-206 expression in mouse PASMCs correlates with an increase in right ventricular systolic pressure. Reduction of miR-206 levels in hPASMCs causes increased proliferation and reduced apoptosis. These results suggest that miR-206 is a potential regulator of proliferation, apoptosis and differentiation of PASMCs, which could yield a novel treatment strategy in PAH.

10. MicroRNAs as biomarkers and therapeutics for asthma (PI: Wang)

MicroRNAs (miRs) are ~22 nucleotides long non-coding RNAs that inhibit mRNA translation by the base pairing rule at the accuracy of one base. It is believed that most human genes and the entire spectrum of biological pathways are tightly and delicately controlled by the miRNome. Deregulation of miRs may contribute to various diseases. The mechanism underlying miR regulations of immunity is under investigation. Developing miR biomarkers and therapeutics for inflammatory diseases, such as asthma, using cell culture and mouse models are goals of this research.

11. LRBA, a novel regulator of immune disorders (PI: Wang)

Lipopolysaccharide (LPS)-responsive beige-like anchor (LRBA) is a novel gene essential for the normal function of the immune system. It is the eighth common variable immunodeficiency (CVID) gene, mutation of which causes CVID and autoimmunity. LRBA is involved in some critical cellular processes such as cell proliferation, apoptosis and autophagy. It may interact with multiple important signal transduction pathways. The molecular mechanism by which LRBA regulates the immune system is being explored.

B. Clinical Research Projects 1. Effects of pine cone extract on IgE levels in patients with allergic

rhinitis (Pl: Ledford)

Pine cones and their aqueous extracts (PCE) were thought to have medicinal properties as far back as 2000 years ago in Japanese populations. Anecdotal reports suggest that the use of PCE improves allergic rhinitis symptoms; it significantly reduces serum IgE levels in mouse models. The purpose of this study is to determine if oral PCE extract administered in a double-blind fashion significantly reduces IgE levels in patients with evidence of perennial allergic rhinitis.

18

2. Pollen and mold counts and immunochemical quantification of outdoor allergens (Pl: Glaum)

Particles other than pollen which transport aeroallergens have been described. The Division, which houses the Pollen and Mold Counting Station for Tampa, has two collectors adapted to collect both pollen and pollen aeroallergens. The collectors are located on the roof of the James A. Haley V.A. Medical Center Research Building. Pollen counts are performed twice weekly, disseminated to local media once weekly and to the Internet twice weekly. Dr. Mary Jelks reads and interprets the slides. The purpose of this study is to determine whether or not there are molecular methods by which aeroallergens can be quantitated and compare them to present day standards assessing pollen counts.

3. Prevalence of food allergy in adult patients with eosinophilic esophagitis (Pl: Ledford)

Food allergies are known to play a significant role in children with eosinophilic esophagitis. Little is known about the prevalence of food allergies in adult patients with this disease. The purpose of this study is to determine the prevalence of food allergies in a cohort of adult patients with eosinophilic esophagitis, results of which will be compared to findings in adult patients with gastroesophageal reflux disease.

4. Effect of oxymetazoline hydrochloride in combination with nasal glucocorticoid on the apnea hypopnea index (AHI) (Pl: Lockey)

“Nocturnal Oxyhemoglobin Saturation, Snoring, and Sleep Quality in Subjects with Persistent Nasal Congestion. A Double Blinded, Placebo Control, Cross Over Prospective Trial” is designed to evaluate the effectiveness on the apnea/hypopnea index (AHI) of adding oxymetazoline to intranasal mometasone, and on other sleep parameters. The study will be carried out in subjects with persistent nasal congestion secondary to allergic or non-allergic rhinitis despite treatment with the highest recommended doses of intranasal mometasone.

5. A study of function of respiration and cognition in the elderly (The FORCE study) (Pl: King)

The purpose of this study is first, to determine which diagnostic tool, impulse oscillometry (IOS), a measurement of respiratory impedance using sound impulses or spirometry, is more feasible for the elderly population to measure pulmonary function; second, to determine if cognitive performance is related to the ability to perform spirometry and IOS; and third, to determine the relationship between biomarkers, cognitive performance, and asthma diagnosis.

6. Evaluation of calcium and vitamin D intake in children on inhaled or intranasal corticosteroids compared to normal children

(Pl: Lockey)

The specific aims of this project are to evaluate the dietary calcium intake of asthmatic children (4-17 years) who are receiving long-term treatment with inhaled or intranasal corticosteroids versus healthy controls using a validated food frequency questionnaire.

19

7. Identification of plasma miRNAs as potential biomarkers in asthma exacerbation (Pl: Lockey)

It is hypothesized that there is a statistically significant difference in miRNA profiling and expression in subjects with asthma exacerbation compared to a baseline level or following effective treatment of an exacerbation of asthma. Therefore, plasma miRNA profiling may provide highly specific and sensitive biomarkers for asthma exacerbation detection and treatment follow-up.

8. Differences in mold counts from January 1995 to December 2011 (Pl: Lockey)

Allergic diseases are due to complex interactions between genetic and environmental factors. Airborne mold and pollens are known to trigger allergic respiratory disease in sensitive individuals. Yet little is known about possible changes as related to climate change in pollen and mold counts over the last 16 years. Daily pollen and fungal spores sample data between January 1995 to December 2010 are available for Sarasota, FL. These data will be compared to weather data for Sarasota available from the National Climatic Data Center. The objectives of this study are to determine if changes in pollen/mold counts can be correlated with climate changes.

C. Clinical Research Unit (CRU)The University of South Florida, Asthma, Allergy and Immunology CRU was established in 1977 to improve the treatment of patients who suffer from asthma, allergic and immunologic diseases. The CRU provides quality research in a variety of clinical areas which include the following: allergic conjunctivitis; allergen immunotherapy; allergen skin testing; allergic rhinitis; asthma; atopic eczema; bronchitis, acute and chronic; contact dermatitis; chronic obstructive pulmonary disease; exercise induced asthma; headache (migraine and tension); hereditary angioedema; immunodeficiency diseases; insect allergy; intravenous immunoglobulin; nasal polyps; sinusitis, acute and chronic; temporomandibular joint disease; urticaria and vasomotor rhinitis. Studies funded by pharmaceutical companies are conducted at the Division’s CRU. The CRU is also a member of the American Lung Association’s Asthma Clinical Research Center network, one of 19 centers throughout the United States.

20

21

IV. BASIC AND CLINICAL RESEARCH SUPPORT

ENDOWMENTSJoy McCann Culverhouse Endowment and Chair in Allergy and Immunology

Mabel and Ellsworth Simmons Professorship for Asthma Research

EXTRAMURAL FUNDINGGovernment Funding

National Institutes of Health (NIAID)National Heart, Lung and Blood Institute

Non-Profit Funding

American Lung AssociationAmerican Heart AssociationAmerican Academy of Allergy Asthma and Immunology, ARTrust

Pharmaceutical Funding (past or present)

Ablynx, NVAlmirall Pharmaceuticals CSL BehringCytosDyax Corporation Forest LaboratoriesGenentech Inc GlaxoSmithKline Jerini, US Merck and Co., Inc.MedImmune Novartis PharmaceuticalsPfizerPharming Inc.RocheSanofi-Aventis Pharmaceuticals Schering-Plough CorporationShireTeva Pharmaceuticals Viropharma 

22

23

V. PUBLICATIONS FROM THE DIVISION

BOOKS PUBLISHED OR IN PRESS

Adelman DC, Casale TB, Corren J (eds): Manual of Allergy and Immunology (5th edition). Lippincott Williams & Wilkins, 2012.

Lockey RF, Ledford DK (eds), in collaboration with the World Allergy Organization: Asthma, Comorbidities, Co-Existing Conditions, and Differential Diagnoses. Oxford University Press, 2014.

Lockey RF, Ledford DK (eds): Allergens and Allergen Immunotherapy: Subcutaneous, Sublingual,and Oral, 5th edition. CRC Press/Taylor & Francis Group, New York, NY, 2014.

Ledford DK, Lockey RF (volume eds: Allergic Diseases): Encyclopedia of Infection and Immunity, (eds, Rose N, Mackay I), Springer, Inc., New York, NY, in press.

BOOK CHAPTERS PUBLISHED OR IN PRESS

Church M, Casale TB: Principles of Pharmacotherapy. In: Allergy, 4th ed. Holgate ST, Church MK, Broide DH, Martinez FD (eds). Elsevier Saunders, pp147-169, 2012.

Lockey RF: Immunotherapy for Venom Allergy Comes of Age. In: Landmark Papers in Allergy. Sheikh A, Platts-Mills T, Worth A (eds). Oxford University Press, Oxford, UK, pp 159-161, 2013.

Lockey RF: Gastroesophageal Reflux Disease and Asthma. In: Global Atlas of Asthma. Akdis CA, Agache I (eds). European Academy of Allergy and Clinical Immunology (EAACI), Section B, Chapter 5, pp 95-99, 2013.

Glaum MC, Lockey RF: Allergen Specific Immunotherapy and Systemic Reactions. In: Anaphylaxis – Principles and Practice. Mamidipudi KT (ed). Nova Science Publishers, New York, 2013.

Dixit N, Glaum M, Kumar A, Cameron DF: Biological Systems for the Delivery of Nanoparticles. In: Nanomedicine in Drug Delivery. Kumar A, Mansour HM, Friedman A, Blough ER (eds). CRC Press/Taylor and Francis Group, 2013.

Casale TB: Severe Asthma. In: Global Atlas of Asthma. European Academy of Allergy and Clinical Immunology (EAACI). Akdis C, Agache J (eds), 2013.

Stokes J, Casale TB: Anti-IgE Therapy . In: Middleton’s Allergy: Principles and Practice, Eighth Edition. Adkinson NF, Jr, Bochner BS, Burks W, Busse WW, Holgate ST, Lemanske RF, Jr, O’Hehir RE (eds). Elsevier, Inc., 2013.

Pesek R, Lockey RF: Diagnosis and Management of Allergy to Hymenoptera (and other insects) Stings in Adolescents and Children. In: Clinical Decision Support: Pediatrics. McMillan JM, Barrett DJ, Boney CM, Jones MD (eds). Decision Support in Medicine, LLC. Wilmington, DE, 2013.

24

Fernández-Caldas E, Puerta L, Caraballo L, Lockey RF: Mite Allergens. In: Allergens and Allergen Immunotherapy: Subcutaneous, Sublingual and Oral, 5th ed. Lockey RF, Ledford DK (eds). CRC Press/Taylor & Francis Group, Boca Raton, FL, pp 181-201, 2014.

Müller U, Golden DBK, Lockey RF, Shin B: Immunotherapy for Hymenoptera Venom Hypersensitivity. In: Allergens and Allergen Immunotherapy: Subcutaneous, Sublingual and Oral, 5th ed. Lockey RF, Ledford DK (eds). CRC Press/Taylor & Francis Group, Boca Raton, FL, pp 401-15, 2014.

Bagg AS, Lockey RF: Adverse Effects and Fatalities Associated with Allergen Skin Testing and Subcutaneous Allergen Immunotherapy. In: Allergens and Allergen Immunotherapy: Subcutaneous, Sublingual and Oral, 5th ed. Lockey RF, Ledford DK (eds). CRC Press/Taylor & Francis Group, Boca Raton, FL, pp 447-59, 2014.

Cox L, Lockey RF: Instruction and Consent Forms for Subcutaneous Allergen Immunotherapy. In: Allergens and Allergen Immunotherapy: Subcutaneous, Sublingual and Oral, 5th ed. Lockey RF, Ledford DK (eds). CRC Press/Taylor & Francis Group, Boca Raton, FL, pp 497-512, 2014.

Fenny N, Dimov VV, Casale TB:. Recombinant and Modified Vaccines and Adjuvants Used for Allergen Immunotherapy. In: Allergens and Allergen Immunotherapy: Subcutaneous, Sublingual,and Oral, 5th ed. Lockey RF, Ledford DK (eds). CRC Press/Taylor & Francis Group, Boca Raton, FL, pp 419-426, 2014.

Rosenwasser LJ, Ledford DK: Antineutrophil Cytoplasmic Antibody – Positive Vasculitis – Comorbid and Coexisting. In: Asthma, Comorbidities, Co-Existing Conditions, and Differential Diagnoses. Lockey RF, Ledford DK (eds), in collaboration with the World Allergy Organization. Oxford University Press, New York, NY, pp 12 - 20, 2014.

Fox RW, Glaum MC: Vocal Cord Dysfunction and Paradoxical Vocal Fold Motion Disorder. In: Asthma, Comorbidities, Co-Existing Conditions, and Differential Diagnoses. Lockey RF, Ledford DK (eds), in collaboration with the World Allergy Organization. Oxford University Press, New York, NY, pp 288-296, 2014.

Pope C, deShazo D, King, MK: Asthma Over 65 – A Disease About Which Little is Known. In: Asthma, Comorbidities, Co-Existing Conditions, and Differential Diagnoses. Lockey RF, Ledford DK (eds), in collaboration with the World Allergy Organization. Oxford University Press, New York, NY, pp 441-52, 2014

Bellamkonda P, Casale TB: Management of Adult Asthma, In: Clinical Asthma: Theory and Practice. Bernstein J, Levy M (eds). CRC Press, 2014.

SCIENTIFIC ARTICLES PUBLISHED OR IN PRESS

Lockey RF (Contributor): Writing Committee for the American Lung Association Asthma Clinical Research Centers: Lansoprazole for children with poorly controlled asthma. JAMA 2012; 307(4): 373-381.

Aljubran SA, Cox R, Parthasarathy PT, Ramanathan GK, Rajanbabu V, Bao H, Mohapatra SS, Lockey RF, Kolliputi N: Enhancer of Zeste Homolog 2 induces pulmonary artery smooth muscle cell proliferation. PLoS ONE 2012; 7(5):e37712.

25

Westermann-Clark E, Fitzhugh D, Lockey RF: Increasing cost of epinephrine autoinjectors. JACI 2012; 130(3): 822-823.

Jalali S, Ramanathan GK, Parthasarathy PT, Aljubran S, Galam L, Yunus A, Garcia S, Cox RR Jr, Lockey RF, Kolliputi N: Mir-206 regulates pulmonary artery smooth muscle cell proliferation and differentiation. PLoS ONE 2012;7(10): e46808.

Elizalde A, Perez EE, Sriaroon P, Nguyen D, Lockey RF, Dorsey MJ: Intensive educational course in allergy and immunology. Allergy 2012; 67(9): 1085-6.

Bousquet J, Heinzerling L, Bachert C, Papadopoulos NG, Bousquet PJ, Burney PG, Canonica W, Carlsen KH, Cox L, Haahtela T, Lodrup Carlsen KC, Price D, Samolinski B, Simons FE, Wickman M, Annesi-Maesano I, Baena-Cagnani CE, Bergmann KC, Bindslev-Jensen C , Casale TB, et al: Practical guide to skin prick tests in allergy to aeroallergens. Allergy 2012;67(1):18-24.

Calderon MA, Cox L, Casale TB, Moingeon P, Demoly P: Multiple-allergen and single-allergen immunotherapy strategies in polysensitized patients: looking at the published evidence. J Allergy Clin Immunol 2012; 129(4):929-934.

Lewis E, Fernandez C, Nella K, Hopp R, Casale T, Gallagher C: Relationship of 25-hydroxyvitamin D and asthma control in children. Ann Allergy Asthma Immunol 2012;108(4):281-282.

Xie Y, Jiang H, Nguyen H, Jia S, Berro A, Panettieri RA, Wolff DW, Abel PW, Casale TB, Tu Y: Regulator of G-protein signaling 2 is a key modulator of airway hyperresponsiveness. J Allergy Clin Immunol 2012; 130(4): 968-976.

Jiang H, Xie Y, Abel PW, Toews ML, Townley RG, Casale TB, Tu Y: Targeting phosphoinositide 3-kinase y [gamma] in airway smooth muscle cells to suppress interleukin-13-induced mouse airway hyperresponsiveness. J Pharmacol Exp Ther 2012; 342(2): 305-311.

Brozek JL Kraft M, Krishnan, JA. Cloutier MM, Lazarus, SC. Li JT, Santesso N, Strunk, RC, CasaleTB: Long- acting beta agonist step-off in patients with controlled asthma – systematic review with meta-analysis. Arch Internal Med 2012; 27: 1-11.

Cox L, Casale TB, Nayak A, Bernstein D, Creticos P, Ambroisine L, Melac, M. Zeldin R: Clinical efficacy of 300IR 5-grass pollen sublingual tablet in a US study: The importance of allergen-specific serum IgE. J Allergy Clin Immunol. 2012;130(6):1327-1334.e1.

Prasanna TP, Phillips O, Kolliputi. N: Thioredoxin catalysis and inflammasome regulation: directions for future research. J Biocatal Biotransformation 2012, 1:2.

Parthasarathy PT, Galam L, Huynh B, Yunus A, Abuelenen T, Castillo A, Kollongod RG, Cox R Jr, Kolliputi N: MicroRNA 16 modulates epithelial sodium channel in human alveolar epithelial cells. Biochem Biophys Res Commun 2012; 426(2): 203–208.

Cox RR Jr, Phillips O, Kolliputi N: Putting the brakes on acute lung injury: can resolvins suppress acute lung injury? Front Physiol 2012; 3:445.

Kolliputi N, Galam L, Parthasarathy PT, Tipparaju SM, Lockey RF: NALP-3 inflammasome silencing attenuates ceramide-induced transepithelial permeability. J Cell Physiol 2012; 227(9):3310-6.

26

Boyapalle S, Wong T, Garay J, Teng M, San Juan-Vergara H, Mohapatra S, Mohapatra S: Respiratory syncytial virus NS1 protein colocalizes with mitochondrial antiviral signaling protein MAVS following infection. PLoS One 2012;7:e29386

Bakre A, Mitchell P, Coleman JK, Jones LP, Saavedra G, Teng M, Tompkins SM, Tripp RA: Respiratory syncytial virus (RSV) modifies microRNAs regulating host genes which affect virus replication. J Gen Virol 2012; 93:2346-56.

Wu W, Tran KC, Teng MN, Heesom KJ, Matthews DA, Barr JN, Hiscox JA: Solution of the human respiratory syncytial virus NS1 protein interactome highlights multiple effects on host cell biology. J Virol 2012: 86:7777-7789.

Hotard AL, Shaikh FY, Lee S, Yan D, Teng MN, Plemper RK, Crowe Jr. JE, Moore ML: A stabilized RNA virus reverse genetics system amenable to recombination mediated mutagenesis. Virology 2012; 434:129-36.

Lang JE, Mougey EB, Allayee H, Blake KV, Lockey R, Gong Y, Hossain J, Killen K, Lima JJ: Nemours Network for Asthma Research: Nutrigenetic response to omega-3 fatty acids in obese asthmatics (NOOA): rationale and methods. Contemporary Clinical Trials 2013; 34(2): 326-35.

Riedl MA, Levy RJ, Suez D, Lockey RF, Baker JW, Relan A, Zuraw BL: Efficacy and safety of recombinant C1 inhibitor for the treatment of hereditary angioedema attacks: a North American open-label study. Annals of Allergy, Asthma and Immunology 2013; 110(4): 295-9.

Berstein JA, Fox,RW, Martin VT, Lockey RF: Headache and facial pain: differential diagnosis and treatment. J Allergy Clin Immunol: In Practice 2013; 1:242-51.

Demoly P, Calderon M, Casale TB, Scadding G, Annesi-Maesano I, Braun, J-J, Delaisi B, Haddad T, Malard O, Trébuchon F, Serrano E: Assessments of disease control in allergic rhinitis. Clin Translational Allergy 2013; 3(1):7.

Maurer M, Rosen K, Hsieh HJ, Saini S, Grattan C, Gimenez-Arnau A, Agarwal S, Doyle R, Canvin J, Kaplan A, Casale TB: Omalizumab for the treatment of chronic idiopathic/spontaneous urticaria. New Engl J Med 2013; 368(10):924-35.

Olsen J, DelasAlas HC, Casale TB: Aquagenic urticaria. J Allergy Clin Immunol:In Practice 2013; 1(3): 295-296.

Calderón MA, Casale TB, Cox L, Akdis AC, Burks AW, Nelson H, Jutel M, Demoly P: Allergen immunotherapy: a new semantic framework from the European Academy of Allergy and Clinical Immunology/American Academy of Allergy, Asthma and Immunology/PRACTALL consensus report. Allergy 2013; 68(7):825-8.

Creticos P, Maloney J, Bernstein DI, Casale TB, Kaur A, Fisher R, Liu N, Murphy K, Nékám K, Nolte H: Randomized controlled trial of a ragweed allergy immunotherapy tablet in North American and European adults. J Allergy Clin Immunol. 2013; 131(5):1342-1349.

Vashisht P, Casale TB: Omalizumab for treatment of allergic rhinitis. Expert Opinion On Biological Therapy 2013; 13(6):933-945.

27

Burks AW, Calderon MA, Casale T, Cox L, Demoly P, Jutel M, Nelson H, Akdis CA: Update on allergy immunotherapy: American Academy of Allergy, Asthma and Immunology/European Allergy and Clinical Immunology/PRACTALL consensus report. J Allergy Clin Immunol 2013;131(5):1288-1296.

Cruz C, Casale TB: Anaphylaxis to cat in a child with exclusive sensitivity to Fel d 1. J Allergy Clin Immunol In Practice 2013;1(4):416-417.

Cox L, Aaronson D, Casale TB, Honsinger R, Weber R: Allergy immunotherapy safety: location matters! J Allergy Clinical Immunology: In Practice 2013:1(5): 455-457.

Lane T, Flam B, Lockey R, Kolliputi N: TXNIP shuttling: missing link between oxidative stress and inflammasome activation. Front Physiol 2013;4:50.

Fukumoto J, Fukumoto I, Parthasarathy PT, Cox R, Huynh B, Ramanathan GK, Venugopal RB, Allen-Gipson DS, Lockey RF, Kolliputi N: NLRP3 deletion protects from hyperoxia-induced acute lung injury. Am J Physiol Cell Physiol 2013; 305(2): C182-9.

Bandyopadhyay S, Lane T, Venugopal R, Parthasarathy PT, Cho Y, Galam L, Lockey R, Kolliputi N: MicroRNA-133a-1 regulates inflammasome activation through uncoupling protein-2. Biochem Biophys Res Commun 2013; 439(3):407-12.

Qazi O, Parthasarathy PT, Lockey R, Kolliputi N: Can microRNAs keep inflammasomes in check? Front Genet 2013;4:30.

Panguluri SK, Tur J, Fukomoto J, Deng W, Sneed KB, Kolliputi N, Bennett ES, Tipparaju SM: Hyperoxia induced hypertrophy and ion channel remodeling in left ventricle. Am J Physiol Heart Circ Physiol 2013; 304(12): H1651-61.

Desai P, Tamarapu Parthasarathy P, Galam L, Lockey R, Kolliputi N: A new role for inflammasomes: sensing the disturbances in non-alcoholic fatty liver disease. Front Physiol 2013; 4:156.

Fukumoto J, Kolliputi N: Human lung on a chip: innovative approach for understanding disease processes and effective drug testing. Front Pharmacol 2013; 3:205.

Grams D, Lockey R, Kolliputi N: Is isoprenylcysteine carboxyl methyltransferase the key to reverse ageing? Front Aging Neurosci 2013; 5:40.

Tian B, Zhao Y, Kalita M, Edeh CB, Paessler S, Casola A, Teng MN, Garofalo RP, Brasier AR: CDK9-dependent transcriptional elongation in the innate ISG response to RSV infection in airway epithelial cells. J Virol 2013; 87:7075-92.

Fuentes S, Crim RL, Beeler J, Teng MN, Golding H, Khurana S: Development of a simple, rapid, sensitive, high-throughput luciferase reporter based microneutralization assay for respiratory syncytial virus. Vaccine 2013; 31:3987-94.

Kolliputi N, Lockey RF: Inflammasome inhibition suppresses alveolar cell permeability through retention of NRG-1. J Immunology, in press.

28

Sandhu MA, Dimov V, Romero FA, Wichman T, Casale TB: Effects of rosiglitazone on airway hyperresponsiveness and obstruction in asthma. Annals Allergy Asthma Immunol, in press.

Jonsson-Razdan P, Casale TB: Sublingual Immunotherapy (SLIT). Ann Resp Med, in press.

REVIEW ARTICLES/EDITORIALS PUBLISHED OR IN PRESS

Butt A, Rashid D, Lockey RF: Do hypoallergenic cats and dogs exist? Ann Allergy Asthma Immunol 2012;108(2): 74-76.

Ring J, Akdis C, Behrendt H, Lauener RP, Schappi G, Akdis M, Ammann W, Lockey RF, et al: Davos Declaration: Allergy as a global problem. Allergy 2012; 67: 141-143.

Calderon MA, Simons FER, Malling HJ, Lockey RF, Moingeon P, Demoly P: Sublingual allergen immunotherapy: mode of action and its relationship with the safety profile. Allergy 2012; 67: 302–311.

Lockey RF: History of the World Allergy Organization: Reflective recollections of the WAO Presidency, 2010 – 2011. WAO Journal 2012; 5(2): 28-35.

Lotvall J, Pawankar R, Wallace DC, Ajdus CA, Rosenwasser LJ, Weber RW, Wesley Burks A, Casale TB, et al: American Academy of Allergy, Asthma & Immunology (AAAA&I) The American College of Allergy, Asthma & Imunology (ACAAI), the European Academy of Allergy and Clinical Immunology, and the World Allergy Organization (WAO): We call for iCAALL: international collaboration for asthma, allergy and immunology. Ann Allergy Asthma Immunol 2012; 108(4):215-216; Allergy 2012; 67(4):449-450; JACI 2012; 129(4):904-5; World Allergy Org J 2012; 5(3):39-40.

Simons FER, Ardusso LRF, Bilo MB, Dimov V, Ebisawa M, El-Gamal YM, Ledford DK, Lockey RF, et al: 2012 Update: World Allergy Organization guidelines for the assessment and management of anaphylaxis. Current Opinion in Allergy & Clin Immunol 2012; 12(4): 389-99.

Lockey RF: The myth of hypoallergenic dogs (and cats). JACI 2012; 130(4):910-11.

Lockey RF (contributor), Sumino K, Holbrook JT, Wise RA, Rogers L: Asthma clinical trials of American Lung Association/Asthma Clinical Research Centers (ALA-ACRC): What have we learned in 12 years? Curr Respir Care Rep 2012; 1(4): 251-258.

Craig T, Pursun EA, Bork K, Bowen T, Boysen H, Farkas H, Grumach AS, Katelaris C, Lockey RF, et al: WAO guideline for the management of hereditary angioedema. WAO Journal 2012, 5:182-199.

Bousquet J, Schunemann HJ, Samolinski B, Lockey RF, et al: ARIA (Allergic rhinitis and its impact on asthma) – achievements in 10 years and future needs. JACI 2012; 130(5):1049-62.

Pawankar R, Canonica W, Holgate S, Lockey RF: Editorial: Allergic diseases and asthma: a major global health concern. Current Opinion in Allergy & Clinical Immunology 2012;12(1):39-41.

29

Ledford DK, Burks AW, Ballow M, Cox L, Wood RA, Casale TB. Statement regarding “The public health benefits of air pollution control”. J Allergy Clin Immunol 2012;130(1):24.

WHO collaborating Center for Asthma and Rhinitis, Bousquet J, Anto JM, Demoly P, Schunemann JH, Toaiga A, Casale TB, et al: Severe chronic allergy (and related) diseases: a uniform approach– a MedALL-GA(2)LEN –ARIA position paper. Int Arch Allergy Immunol 2012; 158(3):216-231.

Ballow M, Akdis CA, Casale TB, Wardlaw A, Wenzel S, Ballas Z, Lötvall J: Immune response modifiers in the treatment of asthma: A Practall document of the AAAAI and EAACI. J Allergy Clin Immunol 2012; 130(2):311-324.

Yorgancıoğlu A, Ozdemir C, Kalaycu O, Kalyocu AF, Bachert C, Baena-Cagnani CE, Casale TB, Chen YZ, Cruz AA, Demoly P, Fokkens WJ, Lodrup Carlsen KC, Mohammad Y, Mullol J, Ohta K, Papadopoulos NG, Pawankar R, Samolinski B, Schünemann HJ, Yusuf OM, Zuberbier T, Bousquet J: ARIA (Allergic rhinitis and its impact on asthma) Achievements in 10 years and future needs. Tuberk Toraks 2012; 60(1):92-7.

Busse WW, Ballas Z, Casale T, Cox L, Dimov V, Honsinger R, Jones C, Tracey J, Whalen K: Future of Allergy: Task Force Report. J Allergy Clin Immunol 2012; 130(4):1009-1010.

Papadopoulos NG, Arakawa H, Carlsen K.-H, Custovic A, Gern J, Lemanske R, Le Souef P, Mäkelä M, Roberts G, Wong G, Zar H, Akdis CA, Bacharier LB, Baraldi E, van Bever HP, de Blic J, Boner A, Burks W, Casale TB, Castro-Rodriguez JA, Chen YZ, El-Gamal YM, Everard ML, Frischer T, Geller M, Gereda J, Goh DY, Guilbert TW, Hedlin G, Heymann PW, Hong SJ, Hossny EM., Huang JL, Jackson DJ., de Jongste JC, Kalayci O, Aït-Khaled N, Kling S, Kuna P, Lau S, Ledford DK, Lee SI, Liu AH, Lockey RF, Lødrup-Carlsen K, Lötvall J, Morikawa A, Nieto A, Paramesh H, Pawankar R, Pohunek P, Pongracic J, Price D, Robertson C, Rosario N, Rossenwasser LJ, Sly PD, Stein R, Stick S, Szefler S, Taussig LM, Valovirta E, Vichyanond P, Wallace D, Weinberg E, Wennergren G, Wildhaber J, Zeiger RS: [ICON] Pediatric Asthma. Allergy 2012; 67(8): 976-997.

Simons FE, Ardusso LR, Bilò MB, Dimov V, Ebisawa M, El-Gamal YM, Ledford DK, Lockey RF, Ring J, Sanchez-Borges M, Senna GE, Sheikh A, Thong BY, Worm M; World Allergy Organization. 2012 Update: World Allergy Organization Guidelines for the assessment and management of anaphylaxis. Curr Opin Allergy Clin Immunol. 2012;12(4):389-99.

Teng MN: The non-structural proteins of RSV: targeting interferon antagonists for vaccine development. Infectious Disorders-Drug Targets 2012; 12:129-37.

Ledford DK, Lockey RF: Asthma and Comorbidities. Current Opinion in Allergy and Clin Immunol 2013; 13(1): 78-86.

Pesek RD, Lockey RF: Management of insect sting hypersensitivity: an update. Allergy, Asthma & Immunology Research 2013; 5(3): 129.

Heinzerling L, Mari A, Bergmannn KC, Bresciani M, Burbach G, Darsow U, Durham S, Fokkens W, Gjomarkaj M, Haahtela T, Bom AT, Wohrl S, Maibach H, Lockey RF: The skin prick test. – European standards. Clinical and Translational Allergy 2013; 3:3.

30

WAO Task Force: Passalacqua G, Baena-Cagnani CE, Bousquet J, Canonica GW, Casale T, Cox L, Durham S, Larenas-Linnemann D, Ledford D, Pawankar R, Potter P, Rosario N, Wallace D, Lockey RF: Grading local side effects of sublingual immunotherapy for respiratory allergy: speaking the same language. JACI 2013;132(1):93-8.

Talreja N, Lockey RF: Case of recurrent exophthalmos: angioedema versus idiopathic orbital pseudotumor. JACI: In Practice 2013; 1(5): 518-19.

Simons FER, Ardusso LRF, Dimov V, Ebisawa M, El-Gamal YM, Lockey RF, Sanchez-Borges M, Genna GE, Sheikh A, Thong BY, Worm M: World Allergy Organization anaphylaxis guidelines: 2013 update of the evidence base. Int Arch Allergy Immunol 2013; 162: 193-204.

Bernstein J, Fox RW, Martin VT, Lockey RF: Headache and facial pain: differential diagnosis and treatment. JACI: In Practice 2013; 1(3): 242-51.

Desai P, Parthasarathy PT, Galam L, Lockey RF, Kolliputi N: A new role for inflammasomes: sensing the disturbances in non-alcoholic fatty liver disease. Frontiers in Physiology 2013; 4:156.

Lane T, Flam B, Lockey RF, Kollipputi N: TXNIP Shuttling: Missing link between oxidative stress and inflammasome activation. Frontiers in Oxidant Physiology 2013; 4:50.

Fukumoto J, Lockey R, Kolliputi N: New hope for Nutlin-3a therapy for pulmonary arterial hypertension. Frontiers in Pharmacology 2013; 4: 87.

Mehta J, Parthasarathy PT, Lockey RF, Kolliputi N: New Hope for a MicroRNA therapy for pulmonary arterial hypertension. Frontiers in Genetics 2013; 4: 137.

Grams D, Lockey RF, Kolliputi N: Is isoprenylcysteine carboxyl methyltransferase the key to reverse ageing? Frontiers in Aging Neuroscience 2013; 5:40.

Qazi O, Tamarapu P, Lockey RF, Kolliputi N: Can microRNAs keep inflammasomes in check? Front Physiol 2013; 4:30.

Stokes J, Casale TB: The relationship between IgE and allergic disease. Up To Date, in press.

INTERNET PUBLICATIONS

Cowan GM, Pesek R: A 3-month old with persistent fever, lymphadenopathy, and hepatosplenomegaly. Interactive case report available at: http://www.worldallergy.org/interactive_case_reviews/. Posted March, 2012.

Fergeson J, Fitzhugh D, Lockey RF: A child with chronic cough. Interactive case report available at http://www.worldallergy.org/interactive_case_reviews. Posted July, 2012.

Butt A, Rashid D, Lockey RF: A persistent rash with urticaria and angioedema following penicillin treatment. Interactive case report available at http://www.worldallergy.org/interactive_case_reviews. Posted August, 2012.

Lockey RF: Disease Summaries – Anaphylaxis: Synopsis. World Allergy Organization website: http://www.worldallergy.org/professional/allergic_diseases_center/anaphylaxis/anaphylaxissynopsis.php. Posted July, 2004. Updated September, 2012.

31

Aljubran SA, Lockey RF: Indoor Aeroallergens. eMedicine from WebMD. Updated November 20, 2012 and December 16, 2013. Available at: http://emedicine.medscape.com/article/137911-overview.

Aljubran SA, Lockey RF: Cold Agglutinin Disease. eMedicine from WebMD. Updated January 25, 2013. Available at: http://emedicine.medscape.com/article/135327-overview

Pepper AN, Aljubran SA, Lockey RF: Recurrent attacks of anaphylaxis. Interactive case report available at: http://www.worldallergy.org/interactive_case_reviews/ Posted May, 2013.

Talreja N, Lockey RF: A case of recurrent exophthalmos. Interactive case report available at: http://www.worldallergy.org/interactive_case_reviews/. Posted December, 2013.

32

33

VI. FACULTY AND STAFF AWARDS

Richard F. Lockey, MD“Member of Honor” award, presented at the Adjuvant & Allergen Vaccines 2012 International Conference by the Cuban Society of Immunology, Havana, Cuba, May 10, 2012

Richard F. Lockey, MDSouthern Medical Society, Dr. Robert D and Alma W. Moreton Orginal Research Award, 2012.

Dennis K. Ledford, MDMabel & Ellsworth Simmons Professor of Allergy and Immunology, 2012

Dennis K. Ledford, MDListed as “Most Compassionate Doctor” – Vitals, 2012

Kolliputi, Narasaiah, PhD WAO Outstanding Abstract Award XXIII World Allergy Organization International Scientific Congress (WISC), Hyderabad, India, December 6– 9, 2012

Kolliputi, Narasaiah, PhDWorld Allergy Organization (WAO) Junior Member Travel AwardHyderabad, India, December, 2012

Dennis K. Ledford, MDElected to the Board of Directors, 2013Joint Council of Allergy Asthma and Immunology

Dennis K. Ledford, MDPresident of Medical Faculty, 2012-2013University of South Florida Morsani College of Medicine

Dennis K. Ledford, MD“25 Years of Service” Award Tampa General Hospital, 2013

Mark C. Glaum, MDAmerican Academy of Allergy Asthma and Immunology (AAAAI)ARTrust Mini Grant, February 25, 2013.

34

35

VII. VISITING PROFESSOR EDUCATIONAL PROGRAM

Adam Wanner, MD, Joseph Weintraub Professor of Medicine, University of Miami Miller School of Medicine, Division of Pulmonary & Critical Care Medicine, Department of Medicine, Miami, Florida. “The Airway Circulation in Asthma”, February 16, 2012

Blanca Camoretti-Mercado, PhD, Department of Medicine, Section of Pulmonary & Critical Care, University of Chicago, Chicago, IL. “Airway Smooth Muscle and Inflammation: Beyond Contraction”, August 2, 2012

Prof. Cezmi A. Akdis, MD, Director, Swiss Institute of Allergy and Asthma Research, Davos, Switzerland. “Tissue Factors that Play a Role in Chronicity and Immune Regulation in Asthma”, September 24, 2012

Hendrik Nolte, MD, PhD, Senior Director, Respiratory and Immunology, Merck Research Laboratories, Kenilworth, New Jersey. “What is the Future of Sublingual Immunotherapy In North America?”, July 25, 2012

Lewis J. Smith, MD, Professor, Pulmonary and Critical Care Medicine, Northwestern University Feinberg School of Medicine, Chicago, Illinois. “The American Lung Association Asthma Clinical Research Network – Accomplishments and Future Studies”, November 15, 2012

Rosa Codina, PhD, Senior Research Scientist , Greer Laboratories, Inc. Lenoir, NC; Clinical Assistant Professor of Medicine, University of South Florida Morsani College of Medicine, Division of Allergy and Clinical Immunology, Department of Internal Medicine, Tampa, FL. “An Introduction to Fungal Allergenic Extracts” and “A Review of a Few Questions to Prepare for the Board Exam”, November 29, 2012

Robert Q. Lanier, MD, Executive Medical Director, American College of Allergy, Asthma & Immunology; Clinical Professor of Pediatrics, University of North Texas Health Science Center, Fort Worth, Texas. “Steroid Footprint: the Cumulative Effects of Steroids in Atopic Patients”, October 10, 2013.

Bruce D. Levy, MD, Associate Professor of Medicine, Harvard Medical School; Associate Physician, Brigham and Women’s Hospital, Boston, Massachusetts. “Pre-resolving mediators in acute lung injury and inflammation”, October 11, 2013.

Dr. Jose Laerte Junior Boechat Morandi, Adjunct Professor, Section of Clinical Immunology, Department of Internal Medicine, Universidade Federal Fliuminense, Rio de Janeiro, Brazil. “Eosinophilic Esophagitis”, November 14, 2013.

David P. Skoner, MD, Children’s Hospital of Pittsburgh, Pittsburgh, PA. “Insights into the Risk Factors, Diagnosis, and Immunologic Mechanisms of Allergic Rhinitis”, December 19, 2013.