MEDICINE OF THE FUTURE STEM CELLS AND GENE THERAPY

IN DIABETES

byProf Morsi Arab

University of Alexandria

The mature B-cells as a source (B-cell

transplantation ) is not satisfactory

because they are :

-Limited in number,

- have very limited proliferation capacity.

- and stimulating their growth by growth factors

diminishes their secretory power.

:Stages of Beta cell development and differentiation

- Fetal Entoderm. cell ( high growth potentiality)

- Panc. Duct epith. Cell

- Committed Endoc. precursor cell

- Growth Stimulated B-cell

- Mature B- cell (V. low division capacity)

GENE THERAPY and STEM CELLSin DIABETES

Gene Therapy is achieved in two steps

i. Prepare The Genetic Material +

ii. Delivery system

Non Embryonic Sources of Stem CellsAdult Bone Marrow Umbilical cord Stem Cells Amniotic Fluid Stem Cells Adult Mesenchymal Stem Cells*….etc

REPROGRAMMING ADULT CELLS BACK TO THE STEM

CELL STATE i.e. Inducing Pluropotency ))

The production of induced PluripotentStem (Cells ( iPSC

By genetic reprogramming of mature adult cells to go back to the pluripotent state (adding genes + transcription factors (TF)

New Developments in Stem Cells Research in Diabetes 2015

- In type 1 Diabetes pancreatic celldestruction results from inflammation + immune reaction

- Adult Mesenchymal Stem Cells ( MSCs) have potent anti- inflammatory and

immune suppressive power .

“Trafficking of Adult Mesenchyme Stem Cells and using Adhesive Molecules towards the Threatened

Pancreatic β cells

saves them from immune destruction”

Thank You