uc davis/nih neufacility romabneuromab.ucdavis.edu/datasheet/n39b_8.pdfno cross-reactivity against...

Anti-GIT1 scaffold protein, NeuroMab clone N39B/8 Immunogen: Fusion protein amino acids 375-770 (C-terminus) of rat GIT1 (also known as G protein-coupled receptor kinase-interactor 1, GRK-interacting protein 1, Cool-associated and tyrosine- phosphorylated protein 1 and Cat-1, accession number Q9Z272) Human: 93% identity (372/396 amino acids identical) Mouse: 97% identity (385/396 amino acids identical) ~50% identity with GIT2 Monoclonal antibody info: Mouse strain: Balb/C Myeloma cell: SP2/0 Mouse Ig Isotype: IgG1 NeuroMab Applications: Immunoblotting, Immunohistochemistry and Immunoprecipitation Species Reactivity: human, mouse, rat No cross-reactivity against GIT2 MW: 90 kD Images Top. Left: immunoblot versus lysates of HEK293T cells untransfected and transfected with Flag-tagged GIT1 and GIT2 plasmids and probed with N39B/8 (top) and anti-Flag (bottom) antibodies. Data courtesy of Eun-Kyoung Hahm, Jaewon Ko, and Eunjoon Kim, Korea Advanced Institute of Science and Technology. Right: adult rat brain membrane immunoblot Bottom. Left: Adult rat hippocampus immunohistochemistry. Right: Immunoblots on brain membranes prepared from whole rat (RBM) and mouse (MBM) brain, and from human cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)]. UC Davis/NIH NeuroMab Facility Department of Physiology and Membrane Biology, UC Davis, Davis CA 95616 http://neuromab.ucdavis.edu [email protected]u © 2020 The Regents of the University of California All Rights Reserved Available as TC supe (RRID: AB_10672300) & Pure IgG (RRID: AB_2109991)

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Page 1: UC Davis/NIH NeuFacility roMabneuromab.ucdavis.edu/datasheet/N39B_8.pdfNo cross-reactivity against GIT2 MW: 90 kD Images Top. Left: immunoblot versus lysates of HEK293T cells untransfected

Anti-GIT1 scaffold protein, NeuroMab clone N39B/8

Immunogen: Fusion protein amino acids 375-770 (C-terminus) of rat GIT1 (also known as G protein-coupled receptor kinase-interactor 1, GRK-interacting protein 1, Cool-associated and tyrosine-phosphorylated protein 1 and Cat-1, accession number Q9Z272) Human: 93% identity (372/396 amino acids identical) Mouse: 97% identity (385/396 amino acids identical) ~50% identity with GIT2

Monoclonal antibody info: Mouse strain: Balb/C Myeloma cell: SP2/0 Mouse Ig Isotype: IgG1

NeuroMab Applications: Immunoblotting, Immunohistochemistry and

Immunoprecipitation Species Reactivity: human, mouse, rat No cross-reactivity against GIT2 MW: 90 kD

Images Top. Left: immunoblot versus lysates of HEK293T cells untransfected and transfected with Flag-tagged GIT1 and GIT2 plasmids and probed with N39B/8 (top) and anti-Flag (bottom) antibodies. Data courtesy of Eun-Kyoung Hahm,Jaewon Ko, and Eunjoon Kim, Korea Advanced Institute of Science and Technology. Right: adult rat brain membrane immunoblot

Bottom. Left: Adult rat hippocampus immunohistochemistry. Right: Immunoblots on brain membranes prepared from whole rat (RBM) and mouse (MBM) brain, and from human cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)].

UC Davis/NIH NeuroMab Facility Department of Physiology and Membrane Biology, UC Davis, Davis CA 95616http://neuromab.ucdavis.edu [email protected]

Available as TC supe (RRID: AB_10672300) & Pure IgG (RRID: AB_2109991)

List of Supplementary Figures and Tables Supplementary Material, Fig. S1: Confirmation that YY1 and NLRP7 directly interact in HEK293T cells. Supplementary

Supporting Information - PNAS · 16/12/2013 · HeLa-S3, HEK293T, human MDA-MB-231 breast adenocar-cinoma, and human MDA-MB-435 breast ... control reporter pTK (Thymidine kinase)-Renilla

A CRISPR-Cas9 gene engineering work- flow: generating ... · CRISPR-Cas9 Gene Engineering platform to knock out PPIB in HEK293T cells. First, co-transfection of the mKate2-Cas9 expression

Supplemental Materials and Methods august corrigé · interaction, HEK293T cells stably expressing the AP2-YFP construct3 were co-transfected with - ... Supplemental Materials and

Supplementary Figure 1 - media.nature.com · Supplementary Figure 1 Activities of ABEs using extended sgRNAs in HEK293T cells. Base editing efficiencies of ABEs with extended sgRNAs

Git branches and remotesdb.cs.duke.edu/.../notes/feb2/git2-spring17.pdf · Merging branches • Sometimes merges fail due to conﬂicts • Git cannot create the diffs to move one

Research Paper Mesenchymal glioblastoma constitutes a ... · PPARG, ACSL1, GIT2 and RAP1B) that interacted with each other in both a ceRNArelated manner and in - terms of their protein

UC Davis/NIH NeuFacility roMab · 2019. 9. 13. · courtesy of Dr. Albert Hung, Picower Institute, MIT. Bottom. Left: Adult rat hippocampus immunohistochemistry. Right. Immunoblots

The cell adhesion-associated protein Git2 regulates ... · Git2 was expressed at relatively high levels at the dome stage, and then exhibited a modest decrease between 50%-90% epiboly

SUPPLEMENTARY MATERIALS Supplemental Tables€¦ · Figure. S2 GIT2 genomic depletion affects functional T cell development and circulating white cell status.WT and GIT2KO total cell