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TRANSCRIPT
Trichoderma Production and Use
in India
Dr. S. Nakkeeran Assoc. Professor
Dept. of Plant Pathology Tamil Nadu Agricultural University
Coimbatore – 641 003
Precision Farming – Brinjal
90 Days old
Profuse bearing
Precision Farming – Bitter gourd Yield :14 t/ha
Income:Rs.1,50,000
Turmeric inter crop onion
Soil Borne Diseases
Wilt
Diverse Microbial community
Discovery in the dirt – Weindling (1932) – Trichoderma lignorum
Biocontrol in India – Introduced - Punjab Mukhopadyay et al. (1980) – Trichoderma
Biocontrol– Introduced – Tamil Nadu - Jeyarajan et al. (1990) – Trichoderma
Trichoderma – The Micro fungi Doing a Macro Role
Trichoderma – Cosmopolitan saprophyte Promote plant health Promote soil health Induce polygeneic resistance Bioremediation Phytobiation
Antagonism – P. aphanidermatum
Antagonism – S. rolfsii
Associated Antimicrobials in Plant Health Alkyl pyrones Pentyl analogues Dermadin Isonitriles Polyketides Peptaibols Steroids Diketo piperazines
Multi hydrolytic enzymes… Cellulase Esterase Proteases Chitinases 1,6 Glucanases 1,3 Glucanases Chitobiosidases N acetyl D glucosaminidase
T.viride T.harzianum
Bio Reactors
Talc pumping unit
Blending unit
Sieving unit
Packing unit
Starter Culture
Blending unit
Packing unit
Growing in TC Bottles
Incubating in racks
Low Cost Technology – Bottle culture technique
Low Cost Technology – Tray culture technique
Low Cost Technology – Bottle culture technique
Use of Trichoderma in India Crop Diseases and Nematodes Biocontrol
Agent
Pigeon pea Fusarium oxysporum f.sp. udum
Macrophomina phaseolina
T. viride (10g/Kg)
Cotton Rotylenchulus reniformis+
M.phaseolina complex
T. viride
(ST + SA)
Red gram
Black gram
Green gram
Heterodera cajani Consortia of Pseudomonas and T. viride (ST)
Black gram Rhizoctonia bataticola T. viride (ST) Green gram Rhizoctonia bataticola T. viride (ST) Cowpea Macrophomina phaseolina
Fusarium oxysporum f.sp. tracheiphilum
T. viride (ST)
Crop Diseases and Nematodes Biocontrol Agent
Chick-pea Fusarium oxysporum f.sp. ciceri Rhizoctonia solani
T. viride
Groundnut Rhizoctonia bataticola
Macrophomina phaseolina
T. viride
Gingelly Macrophomina phaseolina
Fusarium oxysporum f.sp. sesami T. viride
Sunflower Rhizoctonia bataticola T. viride
Cotton Rhizoctonia bataticola
Fusarium oxysporum f.sp. vasinfectum
T. viride
Mulberry Fusarium moniliformae
Macrophomina phaseolina
T. viride
Use of Trichoderma in India
Crop Diseases and Nematodes Biocontrol Agent
Sugarcane Colletotrichum falcatum
Ceratocystis paradoxa
T. harzianum – Sett treatment (10g/Litre)
Tobacco Pythium aphanidermatum T. viride (4g/Kg) Tomato Pythium aphanidermatum
Fusarium oxysporum f.sp. lycopersici
T. viride (10g/Kg) Soil – 2.5 Kg/ha Flowering, fruit formation
Brinjal Pythium aphanidermatum
Rhizoctonia bataticola
T. viride (10g/Kg) Biohardening
Chillies Pythium aphanidermatum
Colletotrichum capsici T. viride (10g/Kg) Biohardening
Betelvine Phytophthora parasitica T. viride (50g/vine in Sorghum based)
Coconut Ganoderma lucidum T. viride (100g/tree)
Use of Trichoderma in India
Crop Disease and Delivery Sys. Dosage
Fenugreek Root rot – R.solani Seed Trt Soil Application
4g/Kg (106 cfu/g) 1.5Kg/ha with 250Kg NC
Black Pepper – Foot rot
Phytophthora capsici – Nursery Pre Plant/pit Field Application
T. harzianum
0.2% drenching 1 Kg NC+ T.V 50g/Pit 50g/vine – Twice
Coconut Ganoderma lucidum Soil Application
100g/tree
Pulses and Oil seeds
Root rot – Seed Trt. Soil Application
4g/Kg 2.5 Kg/ha in 250Kg FYM
Delivery system of Trichoderma
Crop Disease and Delivery Sys. Dosage
Ginger Dry rot – M.phaseolina Rhizome rot - Pythium Soil Application
TH 50g/Plant (Thrice – Multiplied in Sorghum grains)
Cardomum Phytophthora meadii – Field Application
TH or TV 50g/vine – Twice (Sorghum grains)
Turmeric Rhizome rot Soil Application
TH or TV 2.5 Kg/ha + 250 Kg FYM on 3, 5, and 7th month
Cumin and Fenugreek
Root rot – Seed Trt. Soil Application
TH or TV 10g/Kg 2.5 Kg/ha in 250Kg FYM
Delivery system of Trichoderma
Bio-hardening – Trichoderma – Protected Cultivation Mixing in Nursery mix: 1Kg/100 Kg of DCP. Soil Drenching: 5g/Litre
Bio-Fertigation – 1Kg of Trichoderma/1000 sq.ft. Frequency : At monthly intervals. Delivery sysytem : Through Drips
Soil Application – Trichoderma – 2.5 Kg/ha for Annual crops Coconut and Arecanut – 100g/tree Betelvine – 50g/vine Noni – 100g
Training on Biopesticide production and its Impact
Preparation of selective media
Inoculation and culture maintenance
Impact on Biopesticide production
Establishment of Green World Agri Clinic Centre
Preparation of media
Development of Mother culture
Horizontal Spread of technology…
Trichy Kuzhithalai Pettavathalai Cuddalore Ramapuram Chellancherry Theni Madurai Dindigul
Banana Vegetables Paddy Sugarcane Groundnut Sesame
15000 Kg 1000 Km Radius 7900 acres
covered
Registration requirements of biopesticides:
FORMULATION A. Biological Characteristics and Chemistry Systematic name (Genus and species, strain if any) Common name Source and origin Specification of the product Composition of the product Manufacturing process
B. Bioefficacy Results of two trials in two agro climatic conditions
C. Toxicity
a. For primary culture and Formulation - Mammalian Toxicity Testing of Formulations
D. Environmental Safety Testing for formulations: Details on Infection and pathogenicity in mammals i. Non-target vertebrates: Birds (two species), Fresh water fish
ii. Non-target invertebrates Terrestrial invertebrates Soil invertebrates E. Processing, packaging and labeling
Province Place of Production
Kerala Kottayam, Manarcadu, Moonar, Trivandrum, Palghat
Karnataka Mysore, Hyderabad, Secuderabad, Guntar Maharashtra Yavatmal, Pune, Mumbai, Nashik, AP Vihjayawada, Vishalapatnam, Nellore, Rajasthan Rajasthan, Nohar, Jaipur MP Mandasur, Indore West Bengal Medinipur, Bollygunje, Calcutta Gujarat Gujarat Tamil Nadu Coimbatore, Chennai, Theni, Others Varnasi, Ghaziabad, Allahabad
Total Firms
86 – Production capacity: 17200 tonnes/annum
Role of private sectors in spread of technology in India through TNAU
Crop Irrigated Area (ha)
Area under dry land (ha)
Requirement of Trichoderma (Tonnes) Irrigated Dry land
Paddy 1906530 143925 - - Jowar 29818 286456 - 744.78
Bajra 6873 75052 195.14
Ragi 13608 85941 223.45
Maize 59911 142919 371.59
Other cereals
159 40208 104.54
Pulses 29032 496205 81.29 1389.37
Chilies 19826 1270706 99.13 6353.53
Requirement of Trichoderma / annum in Tamil Nadu
Crop Irrigated Area (ha)
Area under dry land (ha)
Requirement of Trichoderma (Tonnes)
Irrigated Dry land
Sugarcane 335382 29207 - - Other food crops
331079 15 0.039
Cotton 43985 65757 - 329.0
Groundnut 189705 429130 - 2574.78
Gingelly 21678 43440 - 217.2
Other Hort.crops
409114 431442 - 2588.65
Total 3396700 969769 180.42 15797
Requirement of Trichoderma / annum in Tamil Nadu
Trichoderma viride: 15977 tonnes/annum
Average Requirement of Trichoderma spp. in India: Four Lakh tonnes
Government Support for the Spread of Technology
Department of Biotechnology Ministry of Science and Technology National Horticultural Mission Mini Mission in Cotton KVK Agricultural Departments NGO SHG NABARD
TOT issues …. Stringent registration formalities should be
relaxed. Provisions should be made for the registration of
consortia based formulations. Village level iPM should be promoted Liquid formulations suited to meet precision
farming systems has to be developed and popularized.
Awareness should be made among the extension functionaries and farming community.
Public private partnership has to be strenthened for the rapid spread of the technology.
Acknowledgement
Support by the Agriculture Office within the Bureau for
Economic Growth, Agriculture, and Trade (EGAT) of the
U.S. Agency for International Development, under the
terms of the Integrated Pest Management Collaborative
Research Support Program (IPM CRSP).