track info 23-3-2009 tu/e 1 master of applied physics physical instrumentation/medical physics track...
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1Track info 23-3-2009
TU/e
Master of Applied Physics
Physical Instrumentation/Medical Physics
Track (specialisatie):
• Ontwikkeling van fysische meetmethoden en -instrumenten voor laboratoria, industrie en biomedische toepassingen
2Track info 23-3-2009
TU/e
Physical Instrumentation/Medical Physics
TPM Transport in Permeable MediaMBx Molecular Biosensors for medical diagnostics
Medical Physics (Klinische Fysica)
3Track info 23-3-2009
TU/e
TPM Transport in Permeable Media
• Meettechniek (vooral op NMR/MRI gebaseerd)
• Vocht- en ionentransport in permeabele media, inclusief diffusie-processen op microscopische en macroscopische schaal
4Track info 23-3-2009
TU/e
Permeabele Media
• Biologische of bio-compatibele weefsels
• (Kunstmatige) membranen, coatings
• Poreuze materialen
Bouwmaterialen Geologische gesteenten, zand, klei Kool, hout, gel Chemische reactoren
5Track info 23-3-2009
TU/e
moisture
mechanical stress
fracture
+ ions
fire freezing drying
boiling crystallization phasetransition
crystal
parameters<- damp water ->
permeabilityhyg
ric
exp
an
sion
6Track info 23-3-2009
TU/e
EPMA
SEM
Invloed magnetische verontreinigingen (vooral Fe)
Korte T2, snel NMR signaalverval
7Track info 23-3-2009
TU/e
NMR/MRI scanners
1.5 Tesla
4.7 Tesla
0.7 Tesla
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TU/e
Relaxatie en diffusie: porie-structuur
porewall
porefluid
t
1t
2
0 1 2 3 4 5 6 7 8 9 10 11 1210
100
1000
10000 bulk water
noise level
sig
nal (
a.u.
)
time (ms)
water in mortar
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80
70
60
50
40
30
20
10
0
101
100
drying time (h
ours)
pore size (nm)
Verdeling vocht over poriën in mortel
0 20 40 60 800.0
0.2
0.4
0.6
0.8
1.0
Sig
nal (
a.u.
)
drying time (hours)
Total gel capillary
10Track info 23-3-2009
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Cryoporometry + NMR relaxation
0.1
1
10
-30 -20 -10 0 10
T2
(ms)
T (°C)fc
mclm Ha
TT
4
pore radius
S
VT 2
large pores
small pores
11Track info 23-3-2009
TU/e
Schade door zout-kristallisatie (Na2CO3)
r crystalΘ
p
clc rP
cos2
PRL 94, 075503 (2005)
0
lnC
C
v
RTP
cc
pore
bulk
12Track info 23-3-2009
TU/e
Dun laagje verf
Paint
Paint
~ 200 µm
~ 100 µm
Top view
Side view
Evaporation
NMR visible
NMR invisible
Sensitive region
Cover glass
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TU/e
High resolution MRI of coatings
0 20 40 60 80 100 120 1400
5
10
15
20
25
30
Sig
na
l In
ten
sity
(a
.u.)
Position (µm)
HardeningEvaporation
& shrinkage
Mobility decrease due to cross-linking yields a faster
NMR signal decay
field gradient 36 T/m
depth resolution 6 μm
14Track info 23-3-2009
TU/e
High resolution MRI of coatings(x
- x
0)2
[μ
m2]
(t - t0) [h]
Air AirArgon
curing process is limited by
oxygen diffusion
)(2)( 000 ttDftf
oxygen density at the surfacefront positionAPL 86, 134105 (2005)
15Track info 23-3-2009
TU/e
Medical Physics / Klinische Fysica
• Vakgebied waar de natuurkundige samen met medische specialisten in een ziekenhuis werkt aan de patiëntenzorg
• Stage- en afstudeerplaatsen binnen ziekenhuizen en de medische industrie
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TU/e
Diffusie- en relaxatie-gewogen hersenscans
diffusie T2
T.g.v. een infarct is de structuur van het weefsel veranderd
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TU/e
Fiber tracking
tibialis anterior muscle
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TU/e
Fiber tracking resultaten
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TU/e
Diffusie gewogen NMR/MRI
0 months0 months 3 months3 months
Neonate with brain injury
fiber tracking results
Rat hippocampus
verification of brain damage model
20Track info 23-3-2009
TU/e
Stage- en afstudeerprojecten
theoriemodelvorming
constructietests
meteninterpreteren
prof.dr.ir. Klaas Kopinga (TPM/KF)prof.dr.ir. Pieter Wijn (KF)dr.ir. Leo Pel (TPM)dr.ir. Henk Huinink (TPM)
Informatie:
Molecular Biosensors for medical diagnostics (MBx)
Research on new sensor principles for the in vitro detection of both
concentration and function of molecules in biological samples
proteins are messengers
in biological systems
biomarkers
give insight in health / disease
10-12 -10-15 M (!)
requires new detection schemes
with low background
probe molecular
binding strength
- relates to affinity
- key to biological function
usebiologicalrecognition
toolbox ofphysics
signal
Biosensors: a system challenge
immuno-assay with or without labels
Research topics:- optical and magnetic detection techniques- particle manipulation with magnetic fields
microfluidic flow
Group investigates magnetic particle labels:
low biological background and possibility for particle manipulation
probing affinities in biochemical systems
example: on-chip manipulation and characterization of magnetic particles
- properties of superparamagnetic particles from Brownian motion in a magnetic potential well
van Ommering et al APL 89 (2006) 142511
optical particle tracking magnetic
potential
particle distributions magnetic susceptibility
current wire
Microscope
Magnetic system
Sample Holder
Force up to 100 pN
Force uniform within 2% over 150 m x 150 m
Setup for pulling
(home build, external magnet)
30
example: affinity probing by magnetic forces
Experiments on model system: Anti-Biotin - biotinylated-BSA
Fm
constant force
quantification
work in progress
proteinantibody from immune system
MBx students usually:
- work at the interface of physics and biochemistry
- apply sensitive optical detection techniques
- develop and realize ideas to manipulate magnetic particles
- develop quantitative models to describe
biochemical processes
For information: dr. Leo van IJzendoorn (N-laag f 1.06)
dr. ir. Arthur de Jong (N-laag f 1.04)
27Track info 23-3-2009
TU/e
PROFIELVAKKEN blok E
3F190 Clinical MRI Wijn E+F 3
3F200 Medical physics Wijn * 3
3F210 Safety and risk analysis Van Kleffens E+F 3
3F220 Radiation protection and radiation dosimetry
Huyskens E+F 3
3F230 Measuring and monitoring of vital functions
Wijn A+B 3
3F240 NMR/MRI Kopinga/Strijkers B+C 3
3F250 Transport in porous media Pel E+F 3
3S100 Magnetism and Magnetic Materials Swagten A+B 3
3S300 Spintronics and nanomagnetism Koopmans E+F 3
3S250 Molecular Photophysics Janssen/Meskers A+B 3
28Track info 23-3-2009
TU/e
PROFIELVAKKEN blok E
3S170 Introduction Nanotechnology Koenraad A+B 3
3S190 Biosensors for medical diagnostics Van IJzendoorn E+F 3
8H010 New developments in fluorescence microscopy on living tissues
Slaaf B+C 3
8N010 Physiological NMR Nicolay D 3
8S070 Clinical Chemistry Vader A+B 3
8S080 Protein engineering Merkx B+C 3
8W090 Cardiovascular fluid mechanics Van de Vosse D 3
8W120 Cell mechanics Bouten ? 3
8W150 Multifluid dynamics Van de Vosse E+F 3