topic: relationships and biodiversity lab aim : how do we gather evidences to determine the species...
TRANSCRIPT
Topic: RELATIONSHIPS AND BIODIVERSITY LAB Aim : How do we gather evidences to determine the species related to Botana curus?
DO NOW: (5 MINUTES) NOTE: READ AND ANSWER THE QUESTIONS BELOW.
Botana curus is a valuable plant because it produces CUROL, a substance used to treat certain type of cancer. Curol is not produced in the laboratory. Botana curus rows very slowly and is on the endangered species List. Species that are most closely related to Botana curus might produce Curol. These 3 similar plant species are SPECIES X, SPECIES Y andSPECIES Z. One of them is closely related to Botana curus and could able to produce curol.
QUESTIONS: 1. Why is Botana curus considered as a valuable plant?2. What is curol?3. What are the problems about Botana curus
AGENDA 03/04/09 DO NOW: • Silent ReadingMINI LESSON:• Gathering structural Evidences to prove relationships• Formulating hypothesis ACTIVITY: • Observing structural characteristics of Botana curus and species XYZ REFLECTIONHOMEWORKCastle Learning
Botana curus is a valuable plant because it produces Curol, a compound used for treating certain kinds of cancer. Curol can not be produced in the laboratory.
Botana curus grows very slowly and is on the endangered species list, so its ability to provide curol in large quantities is limited.
Topic: RELATIONSHIPS AND BIODIVERSITY LAB Aim : How do we gather evidences to determine the species related to Botana curus? AGENDA 12/18/0 DO NOW: • Silent ReadingMINI LESSON:• Gathering structural Evidences to prove relationships• Formulating hypothesis ACTIVITY: • Observing structural characteristics of Botana curus and species XYZ REFLECTIONHOMEWORKCastle Learning
MINI LESSON: (10 to 15 mins ) AGENDA 03/04/09 DO NOW: • Silent ReadingMINI LESSON:• Gathering structural Evidences to prove relationships• Formulating hypothesis ACTIVITY: • Observing structural characteristics of Botana curus and species XYZ
REFLECTIONHOMEWORK
Castle Learning
Topic: RELATIONSHIPS AND BIODIVERSITY LAB Aim : How do we gather evidences to determine the species related to Botana curus? AGENDA 12/18/08 DO NOW: • Silent ReadingMINI LESSON:• Gathering structural Evidences to prove relationships• Formulating hypothesis ACTIVITY: • Observing structural characteristics of Botana curus and species XYZ REFLECTIONHOMEWORKCastle Learning
AGENDA 03/04/09 DO NOW: • Silent ReadingMINI LESSON:• Gathering structural Evidences to prove relationships• Formulating hypothesis ACTIVITY: • Observing structural characteristics of Botana curus and species XYZ
REFLECTIONHOMEWORK
Castle Learning
TABLE 1: STRUCTURAL EVIDENCES SPECIES Structural
Characteristics of Plant(color of leaf&petals)
Structural Characteristics of Seeds(color and shape of seeds,)
MicroscopicStem Structure(scattered or circular)
BOTANA CURUS
SPECIES X
SPECIES Y
SPECIES Z
Topic: RELATIONSHIPS AND BIODIVERSITY LAB Aim : How do we gather evidences to determine the species related to Botana curus? AGENDA 12/18/08 DO NOW: • Silent ReadingMINI LESSON:• Gathering structural Evidences to prove relationships• Formulating hypothesis ACTIVITY: • Observing structural characteristics of Botana curus and species XYZ REFLECTIONHOMEWORKCastle Learning
AGENDA 03/04/09 DO NOW: • Silent ReadingMINI LESSON:• Gathering structural Evidences to prove relationships• Formulating hypothesis ACTIVITY: • Observing structural characteristics of Botana curus and species XYZ
REFLECTIONHOMEWORK
Castle Learning
Topic: RELATIONSHIPS AND BIODIVERSITY LAB Aim : How do we gather evidences to determine the species related to Botana curus? AGENDA 12/18/08 DO NOW: • Silent ReadingMINI LESSON:• Gathering structural Evidences to prove relationships• Formulating hypothesis ACTIVITY: • Observing structural characteristics of Botana curus and species XYZ REFLECTIONHOMEWORKCastle Learning
Structural Characteristic of Plants AGENDA 03/04/09 DO NOW: • Silent ReadingMINI LESSON:• Gathering structural Evidences to prove relationships• Formulating hypothesis ACTIVITY: • Observing structural characteristics of Botana curus and species XYZ
REFLECTIONHOMEWORK
Castle Learning
Topic: RELATIONSHIPS AND BIODIVERSITY LAB Aim : How do we gather evidences to determine the species related to Botana curus? AGENDA 12/18/08 DO NOW: • Silent ReadingMINI LESSON:• Gathering structural Evidences to prove relationships• Formulating hypothesis ACTIVITY: • Observing structural characteristics of Botana curus and species XYZ REFLECTIONHOMEWORKCastle Learning
Structural Characteristic of Seeds AGENDA 03/04/09 DO NOW: • Silent ReadingMINI LESSON:• Gathering structural Evidences to prove relationships• Formulating hypothesis ACTIVITY: • Observing structural characteristics of Botana curus and species XYZ
REFLECTIONHOMEWORK
Castle Learning
Compare Stem Structures
Botana curus
Species X
Species Y Species Z
Topic: RELATIONSHIPS AND BIODIVERSITY LAB Aim : How do we gather evidences to determine the species related to Botana curus?
AGENDA 12/18/08 DO NOW: • Silent ReadingMINI LESSON:• Gathering structural Evidences to prove relationships• Formulating hypothesis ACTIVITY: • Observing structural characteristics of Botana curus and species XYZ REFLECTIONHOMEWORKCastle Learning
Microscopic Stem Structure- SCATERRED OR CIRCULAR?
AGENDA 03/04/09 DO NOW: • Silent ReadingMINI LESSON:• Gathering structural Evidences to prove relationships• Formulating hypothesis ACTIVITY: • Observing structural characteristics of Botana curus and species XYZ
REFLECTIONHOMEWORK
Castle Learning
HYPOTHESIZE: TESTS 1-3
A. Base on your data for structural relationships, which species ( X,Y,Z) would you hypothesize is most likely to produce CUROL? __________
B. Explain how the evidence from your data table supports your hypothesis.
Topic: RELATIONSHIPS AND BIODIVERSITY LAB Aim : How do we gather evidences to determine the species related to Botana curus?
AGENDA 12/18/08 DO NOW: • Silent ReadingMINI LESSON:• Gathering structural Evidences to prove relationships• Formulating hypothesis ACTIVITY: • Observing structural characteristics of Botana curus and species XYZ REFLECTIONHOMEWORKCastle Learning
Formulating Hypothesis AGENDA 03/04/09 DO NOW: • Silent ReadingMINI LESSON:• Gathering structural Evidences to prove relationships• Formulating hypothesis ACTIVITY: • Observing structural characteristics of Botana curus and species XYZ
REFLECTIONHOMEWORK
Castle Learning
TABLE 1: MOLECULAR EVIDENCES
SPECIES Paper Chromatography(color)
Test for ENZYME M (present / absent)
Differences in Amino Acid Sequences
Gel Electrophoresis Banding Pattern
B C
SPECIES X
SPECIES Y
SPECIES Z
Topic: RELATIONSHIPS AND BIODIVERSITY LAB Aim : How do we gather evidences to determine the species related to Botana curus? AGENDA 12/18/08 DO NOW: • Silent ReadingMINI LESSON:• Gathering structural Evidences to prove relationships• Formulating hypothesis ACTIVITY: • Observing structural characteristics of Botana curus and species XYZ REFLECTIONHOMEWORKCastle Learning
AGENDA 03/04/09 DO NOW: • Silent ReadingMINI LESSON:• Gathering structural Evidences to prove relationships• Formulating hypothesis ACTIVITY: • Observing structural characteristics of Botana curus and species XYZ
REFLECTIONHOMEWORK
Castle Learning
TEST # 4: Paper Chromatography
Topic: RELATIONSHIPS AND BIODIVERSITY LAB Aim : How do we gather evidences to determine the species related to Botana curus?
AGENDA 03/04/09 DO NOW: • Silent ReadingMINI LESSON:• Gathering structural Evidences to prove relationships• Formulating hypothesis ACTIVITY: • Observing structural characteristics of Botana curus and species XYZ
REFLECTIONHOMEWORK
Castle Learning
Testing for “M”
Record the results of your tests for enzyme “M”
(either a Positive or Negative result) in Table 1
Botana curis
Species X
Species Y
Species Z
Topic: RELATIONSHIPS AND BIODIVERSITY LAB Aim : How do we gather evidences to determine the species related to Botana curus?
Base Pair Rule
Topic: RELATIONSHIPS AND BIODIVERSITY LAB Aim : How do we gather evidences to determine the species related to Botana curus?
DNA : GCAT GAA GGA CTCCTT CCT GAG
RNA : GCAU CAC GTG GTA
GUG CAC CAU
Translating DNA Code to Make Proteins
BOTANA CURUS CAC GTG GAC TGA GGA CTC CTC
Sequence of bases in mRna Produced ______________________________
Sequence of amino Acids in the protein _______________________________
GUGCAC CUG ACU CCU GAG GAG
VAL HIS LEU THR PRO GLU GLU
Topic: RELATIONSHIPS AND BIODIVERSITY LAB Aim : How do we gather evidences to determine the species related to Botana curus?
Topic: RELATIONSHIPS AND BIODIVERSITY LAB Aim : How do we gather evidences to determine the species related to Botana curus?
Gel Electrophoresis
Topic: RELATIONSHIPS AND BIODIVERSITY LAB Aim : How do we gather evidences to determine the species related to Botana curus?
Separation of DNA Fragments
GEL ELECTROHORESIS BANDING PATTERN
BC: ATTCCGGATCGATCGCCGGATATACTCCGGTTATATC
5- 12- 11- 9
X : ATTGTACCGGGATCCGGACGTCGCGACTAATATAGCA
8- 7- 22 Y : ACCGGTCCGGGATCGCACCCGGTACTCCTGTAATATC
3 5 12- 17 Z : ATTCCGGATCGATCGCCGGATATACTCCGGTTATATC
5- 12- 11- 9