tissue culture of nephentes mirabilis and nephentes ampullaria
TRANSCRIPT
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Kultur Jaringan Kantong SemarNephentes mirabilis dan Nephentes
ampullaria
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Kelompok 3Destri Apriani (13222025)Dewi Sundari (13222029)Evitia Yuliani (13222039)Rapida Juliana (12222086)Rika Damayanti (12222090)Roris Agafta (12222093)
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Jurnal Kultur Jaringan
Kultur Jaringan Kantong Semar (Nepenthes mirabilis) Oleh : Dini Dinarti DKK
Induksi Pembentukan Kantong dan Pertumbuhan Dua Spesies Tanaman Kantong Semar ( Nepenthes Spp.) Pada Berbagai Konsentrasi Media Ms Secara In Vitro Oleh : Fitri Yelli
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Klasifikasi Kantong SemarRegnum : PlantaeDivisio : SpermatophytaKelas : DikotilOrdo : CaryophyllalesFamilia : NepenthaceaeGenus : NepenthesSpesies : Nepenthes mirabilis, Nepenthes
ampullaria
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Nephentes mirabilis
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Penampang Biji dan Kantong Nephentes mirabilis
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Nephentes ampullaria
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Penampang Biji dan Kantong Nephentes ampullaria
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Perkecambahan Nephentes mirabilis
Percobaan 1Perbedaan jenis media &
konsentrasi
Percobaan 2Perlakuan dalam faktor
tunggal
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Bahan yang Digunakan pada Percobaan 1
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Percobaan 1Perbedaan jenis Media & Konsentrasi
Murashige and Skoog (MS) dan Knudson C (KC). 4 taraf konsentrasi yaitu 1, ¾, ½ dan ¼ konsentrasi garam
media MS atau KC.
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8 Kali Kombinasi dan 3 Kali Pengulangan Untuk Setiap Media dengan 10 Biji
M 1
M 1
M 1
M 3/4 M 1/2 M 1/4
M 3/4
M 3/4
M 1/2
M 1/2
M 1/4
M 1/4
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Murashige and Skoog (MS)
Konsentrasi Murashige and Skoog (MS) 1
Konsentrasi Murashige and Skoog (MS) 3/4
Konsentrasi Murashige and Skoog (MS) 1/2
Konsentrasi Murashige and Skoog (MS) 1/4
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Knudson C (KC)
KonsentrasiKnudson C (KC)
1
Konsentrasi Knudson C
(KC) 3/4
Konsentrasi Knudson C
(KC) 1/2
Konsentrasi Knudson C
(KC) 1/4
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8 Kali Kombinasi Dan 3 Kali Pengulangan Untuk Setiap Media Dengan 10 Biji
K 1
K 1
K 1
K 3/4 K 1/2 K 1/4
K 3/4
K 3/4
K 1/2
K 1/2
K 1/4
K 1/4
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Percobaan 2perlakuan dalam faktor tunggal
½ MS
¼ KC
½ MS + 0.01 ppm
Thidiazuron (TDZ)
¼ KC + 0.01 ppm
TDZ
½ MS + 0.1 ppm
IAA
¼ KC + 0.1 ppm
IAA
½ MS +10 ppm
GA3
KC+10 ppm GA3
½ MS +150 ml
air kelapa
¼ KC + 150 ml air
kelapa
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Sterilisasi pada Percobaan Pertama dan Kedua• Merendam biji ke dalam isopropil alkohol
selama 5 menit .• Dibilas air steril. • Biji direndam dalam clorox 10% selama 2-
4 menit lalu dibilas sampai bersih. • Biji direndam dalam H2O2 3% selama 1
menit tanpa dibilas air steril.• Ditanam sebanyak 10 biji per botol kultur.
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Biji Kantong Semar
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Foto Tahapan Setrilisasi
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Tahapan Pembuatan Media dan Penyimpanan
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Pertumbuhan Nephentes mirabilis
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Pengaruh Konsentrasi Media terhadap Persentase Biji Berkecambah Nephentes mirabilis
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Pengaruh Jenis Media terhadap Persentase Biji Berkecambah Nephentes mirabilis
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Interaksi jenis dan Konsentrasi Media terhadap Waktu Berkecambah Biji Nephentes mirabilis
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Dari Percobaan Dapat Diketahui
Perkecambahan Nephentes
mirabilis
TDZ pada ¼ KC dan GA3 pada ½ MS
persentase benih Nepenthes mirabilis
(93–97%)
Media KC terbaik dalam menginduksi persentase kultur
Nepenthes mirabilis berkecambah (64%)
Media ½ atau ¼ konsentrasi
meningkatkan persentase biji Nepenthes mirabilis
berkecambah (67–78%).
Pemberian TDZ pada ½ MS dan ¼ KC, GA3
pada ¼ KC mempercepat waktu berkecambah (27 –
33 hari setelah semai).
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Induksi Pembentukan Kantong Dan Pertumbuhan
Dua Spesies Tanaman Kantong Semar (Nepenthes Spp.) Pada Berbagai Konsentrasi
Media Ms Secara In Vitro
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Penampilan baik
Pertumbuhan seragam
Ditanam pada media perlakuan
Satu eksplan untuk 1 botol kultur
Bahan yang Digunakan
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Tunas Kantong Semar
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MediaGaram mineral
Bahan-bahan
organik
(MS)
Vitamin
Sukrosa (30 g/l)
HCL (1N) atau
NaOH (1N).
Agar swallow
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Bahan yang Digunakan
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Langkah Kerja• Dilarutkan dengan pengadukan dan
pemanasan.• Dituang ke dalam botol kultur berkapasitas
300 ml.• Ditutup dengan plastik bening.• Botol kultur disterilkan selama 7 menit
dalam autoklaf.
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Perlakuan Media MS0,500 MS yaitu media MS dengan ½ unsur makro.
0,250 MS yaitu media MS dengan ¼ unsur makro.
0,125 MS yaitu media MS dengan 1/8 unsur makro.
0,0625 MS yaitu media MS dengan 1/16 unsur makro.
0,000 MS yaitu media yang hanya terdiri atas air, gula dan agar.
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Media setiap Botol Kultur
0,500 MS 0,250 MS 0,125 MS
0,000 MS 0,0625 MS
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Perlakuan yang Dilakukan pada Nephentes mirabilis dan Nephentes ampullaria
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Fokus Pengamatan selama 2 minggu – 12 minggu
Jumlah Daun
Jumlah Kantung
Tinggi Tanama
n
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Hasil Pengamatan• Kantong mulai terbentuk pada saat umur tanaman
mencapai 4 minggu.• Hampir semua daun memiliki tendril pada ujungnya,
namun tidak semua tendril mampu membentuk kantong.
• Proses pembentukan kantong pada tanaman Nepenthes berbeda-beda.
• Daun tanaman N. mirabilis berbeda dengan N. ampullaria.
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Gambar Tendril dan Kantong pada Kantong Semar
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Perbedaan Morfologi Daun N. mirabilis dengan N. ampullaria.
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Jumlah Daun• Minggu 1-12, jenis nepenthes mempengaruhi
jumlah daun.• Pengaruh dari berbagai konsentrasi media MS
baru terlihat pada umur 4 minggu.• Nepenthes mirabilis menghasilkan 9,63
daun/tanaman.• Nepenthes ampullaria sebesar 7,80
daun/tanaman.
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Tabel Pertumbuhan Jumlah Daun
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Tabel Pertumbuhan Jumlah Kantong
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Dapat Diketahui
Pertumbuahan Ksntong
Tidak ada interaksi antara jenis nepenthes
dengan konsentrasi media tumbuh.
Jenis Nepenthes mempengaruhi jumlah
kantong tanaman
Pada 12 MST jumlah kantong pada kedua
spesies tidak berbeda.Jumlah kantong tertinggi di peroleh pada umur 12 MST
yaitu 4,37 kantong/ tanaman pada N. ampullaria4,50 kantong/
tanaman pada N. mirabilis
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Penampilan Kantong N. ampullaria Setiap Media Tanaman
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Penampilan Kantong N. mirabilisSetiap Media Tanaman
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• Warna kantong pada media 0,00 MS menguning mengikuti warna daunnya.
Keadaan Daun pada Media 0,00 Ms
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Pengaruh Jenis Kantung Semar dan Konsentrasi terhadap Tinggi Tanaman
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Terima Kasih