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© Thrive Bioscience, Inc. 2021

Analytics.

Automation.

BetterBiology.

ThriveBioscience

IntroductiontoCompany&Products

February22,2021

“50FastestGrowingCompaniesof2020”-- SiliconReview“20BestBiotechCompaniesof2019”-- CIOReview

2© Thrive Bioscience, Inc. 2021

IntroductiontoThrive


1952- 2020

2020+

CurrentStatusofCellCulture:• Cellcultureisatthecenterofbio-medicalresearch--$22billionperyearmarket.

• Cellcultureisconductedsimilarlyto65yearsago--significantunsolvedpainpointsandlimitedinnovation.

• Cellsvarysignificantlyinqualityacrosslabs&experiments--highratesofmislabeling,cross-contaminationandstress.

Result:Billionsofdollarsarespenteachyearonassaysandinstrumentstostudycellsofvariableandoftenpoorquality.

ThriveBioscience’sSolution:Instrumentsandsoftwarethatprovideimaging,analytics,andautomationforreproduciblecellculture.

ThriveInstruments

Goodscienceneedsgoodcells!


ü CommercializationofFamilyofInstrumentsandSoftware-- forsmall,medium&largelabswithcommondatabaseandanalyticso CellAssistlaunchedlate2020(12sitesinUS,Canada,JapanandKorea)o CellAssist50startedshippingDecember(2sites)o HiredCommercialTeamAugusttoSeptember

ü StrategicCollaborations --o Collaborationwith$18billionbigpharmafordevelopmentofversionsofThriveinstrumentsforbioprocessing&celltherapies

o AcademiccollaborationsforsoftwareapplicationswithHarvardStemCellInstitute,MassachusettsGeneralHospitalCenterforGenomicsMedicineandUniversityofTexasMedicalGalveston

ü LimitedCompetition/ExtensiveIPPortfolio -- 64applications(ofwhich14issued)coveringcell,tissue&pathogenculture,IoT,blockchain,andAIforresearchandbioprocessing

ü $25.4MillionofFinancingRaisedTo-date--o Familyoffices(LauderPartners,MilsteinFamily,ParkerBrosFamily)o Angelgroups(LandmarkAngels,LifeScienceAngels,SideCar Angels)o

o Industryexecutives(formerCEO’sofNovartis,Cytyc,ExactSciences,BrammerBio,Patehon)

ü Financing --SeriesB-- $12Mtotal,startinginitiallywith$2MconvertiblenotetocloseinApril;useofproceedsforsalesexpansion,developmentofsoftwareapplications,and3rd instrumentengineering

ThriveBioscience-- KeyPoints

CellAssist(2020)

CellAssist50(2021)

CellCultureManagementSystem

(2023)


LeadershipTeam

ThomasForestFarb-HorchPresident,Co-FounderandBoardDirector

o PresidentandCOOofIndevusPharmaceuticals(acquiredbyEndo)

o VPofCorporateDevelopmentofCytyc(acquiredbyHologic)

o Founder/BoardMemberofHNCSoftware(neuralnetworks/acquiredbyOracleandFairIsaac),ExactSciences(NASD:EXAS)

Alan-PhilippeBlanchardPh.D.,CSOCo-FounderandBoardDirector

o FoundingMemberofRosettaInpharmatics(acquiredbyMerck)

o ResearchFellow,LifeTechnologies/Thermo Fisher

o SeniorFellow,LeroyHoodLaboratory,UniversityofWashington

TakaKiyoizumi,M.D.,Ph.D.EVPofInternational

o FounderandCEO,MediciNova(NASDAQ:MNOV)

o PresidentandCEO,TanabeResearchLaboratories,USA,USaffiliateofMitsubishiTanabePharma

HerbKenny,EVPofCommercialization

o VPofCommercialOperations,ScientificInstruments,Thermo Fisher($2billionofsales)

o 34yearsinglobalsales,serviceandmarketingexperience

JeffGoldman,M.S.VPofEngineeringandManufacturing

o R&DManager,SeahorseBioscience(acquiredbyAgilent)

o EngineeringDirector,454LifeSciences(acquiredbyRoche)

o ProductDevelopmentDirectorofMJResearch(acquiredbyBio-Rad)


GuyBroadbentChairofBoard

o FormerPresident,ThermoFisherScientific,LaboratoryProductsDivision

o FormerCEO,XcellereX(acquiredbyGELifeSciences)nsArgotec(acquiredby

Schweitzer-Mauduit)

o BoardMemberatAdvancedInstruments,AquapakPolymers(Chair),Aurora

PlasticsandClockSpring|NRI(Chair)

MichaelFinney,Ph.D.(MIT,Biology)

o ManagingDirectorofFinneyCapitalandactiveMemberofLifeScienceAngels

o FormerCSO,MJResearch(acquiredbyBio-Rad)andCEO,Vaxart

o BoardMemberatTheGenomePartnership,InnerscopeResearch,OrionGenomics,SageScience(Chair)andVaxart

BrockReeve,MPhil(Yale),MBA(Harvard)

o ExecutiveDirector,HarvardStemCellInstitute

o CEOofIVIVAMedical

o FormerManagingDirectorandCOOofLifeScienceInsights,anIDCcompany

o FormerHealthcarePracticeDirectorofViantandPrincipalofSRIConsulting

OutsideBoardofDirectors


Thrive’sSolution-- FamilyofCellCultureInstruments&Software

CellAssistOnMarket

Bench-topimaging&analytics

• 12Customer&CollaboratorSites

• $95,000list(U.S.)

CellCultureSystemAvailable2023

Walk-awayintelligent100platecellculture

• Inalphatest

• 1,050,000list(U.S.

CellAssist50EarlyAccessOnMarket

24/7AutomatedCellAssistfor50plates

• 2Customer&CollaboratorSites• IntegratestheCellAssist,robotics,&anautomatedincubator

• $275,000list(U.S.)

7mx.7mx1.7m 2.4mx1.0mx1.8m

Integratesimaging,robotics,fluidics&machinelearningtogrowhighqualityadherentcellsconsistently

o Commondatabase,imageprocessing&modulesacrossproductso NoFDAclearanceorapprovalrequired

(.5mx.5mx.6m)

SoftwareModulesAvailable2021- 2023

Stand-AloneApplicationPackagesSolvingMajorProblemsinCellCulture

• Organoids&3DCulture

• SingleCell&SingleColonyTracking

• InfectiousDiseaseRapidViralPlaqueAssays

• StemCellDifferentiationDetection&Monitoring


CellAssist Family-- TheRightDataandLotsofDataforCellBiology

AnalyticWorkstationinLaborOffice

CellAssistImagerinLab

• 6-wellto96-wellplates• 4x&10xmagnification• Phasecontrastandbright-fieldmodes

• Singleand50-plateversions

ImageCollectionExample6-WellPlateat4x

• 1,470imagesin10minsü 7z-heights@20µmincrementsü 35imagetilesperwellateachz-height

ü 6wells

• ImageRegistrationü 15micronsbetweenscans(afterremovingandreloadingplate)

•GenomicsQuantityofDataNowAvailableforCellBiologyü 7.4Gbdata-setperplatescanü 1.0TBpermonthformanylabs

Data.Analytics.

Documentation.ReproducibleCellCulture.


ExampleSoftwareApplicationsforCellAssist Family

Examplesofsoftwaremodulestobesoldinadditiontotheinstrumentsinclude:

Ø Viralplaqueassays(plaquesvisiblein24to36hours)

Ø Organoid/spheroidimagingØ Automaticdeterminationofmonoclonalityorpolyclonality ofstemcellcolonies

Ø Automatictrackingofsinglecoloniesorcells

ViralPlaqueAssays:

Organoid/SpheroidImaging:

#1: 24-Dec-2018 #2: 31-Dec-2018 #3: 07-Jan-2019 #4) 14-Jan-2019 #5) 22-Jan-2019

Day2 Day4 Day6

ClonalityDetermination:

SingleColony&SingleCellTracking:


CellAssist50ScreenShot(exampleofimagingeveryfourhours)


BasicPre-ClinicalPilotProcessYield&QualityResearchDevelopmentProductionOptimizationCost

CellAssist significantlyimprovesmanyworkflowsfromresearchtobioprocessing

Analytics. Automation. Better Biology.

ü Fitsintocurrentdailylabworkflowwhenexaminingcells

ü Morethoroughlyimages&inspectscells&identifiesproblems

ü Automaticallydocumentswhatisrarelycapturedcurrently-- who,what,when,where,how

ü Buildsadatabaseofcells,experimentsandresults

ü Enableslabstoreview&verifywithin&acrosslabs

CellAssist FamilyCapabilities&Benefits


CellCulture-- $18.6BillionMarketPerYearin2020Worldwide• 72%Pharma/Biotech/Cosmetics/Industrial• 28%Research/AcademicLabs

CellCultureMarketSizeandApplicationsofThriveInstruments

CancerResearch

VaccineResearch

VirusResearch(CoV,RSV, HIV, Zika)

DrugDiscovery

StemCellResearch

ArtificialOrgans

RegenerativeMedicine

ScreeningonCellLines

Immuno-therapy

CellAssist Enables• Precisemeasurementsofcellcharacteristics

• Engineeredcells• Autologoustherapies• Buildingmulti-cellstructures

RelevantFieldsofLifeSciences RelevantUsesinLifeSciences


ExampleCustomerApplications


CellAssistimprovesqualityanddocumentationofcells

(statistical anomaly highlighted)

MislabeledCellCulturePlates

CustomerApplication:AnalysisofOutlierstoImproveQuality

Problem:Currenttoolsdonotcollectimagesanddataalongwithtoolsthatenableuserstovisualizeoutliers.Outliersareimportantbecausetheymayindicateproblemsinqualitythatrequireinvestigation.Objective:Documentandanalyzeimagesanddataoncellsovertimetoimprovequalityandreproducibilityofcellculture.CellAssistSolution:CellAssistprovidescomparabledataovertimethatallowsforidentificationofoutliers.(Inthisexample,uponfurtherinvestigation,thecustomerfoundcasesofmislabeling.)


CellAssist providesobjectivemetricsandimagesforconsistentdecision-making

Problem:Individualsvaryintheirdeterminationofconfluence.Further,particularlywhenusingbright-field,theymayignoretheouterringofthewells.Objective:Standardizemeasurementofconfluence.CellAssistSolution:CellAssistobjectivelymeasuresconfluenceinthecenterandmoredifficulttoreadouterringofthewell.

CustomerApplication:ConfluenceinCenterandOuterRings


Problem:Geneeditingtransfectionratescanbepoor,soitisimportantthatmonoclonalcoloniesbeselectedforpassaging.Objective:Accuratelydeterminewhetheracolonyismonoclonalbycapturingseriesoftimelapseimageswithexcellentregistrationacrossscans.CellAssistSolution:TheCellAssisttime-lapseimagingenablesthelineageofcandidatecoloniestobetrackedtoasinglestartingcell.

Day2 Day4 Day6

Seevideooftimelapseimagesthatallowresearcherstoselectmonoclonalorpolyclonalcolonies:https://vimeo.com/348386015/dc51c9038c

CellAssistimprovesqualityandincreasesyieldsbyusingdatatogradeandpickcolonies

CustomerApplication:SingleColonyTracking&MonoclonalityProbabilityDetermination

https://vimeo.com/348386015/dc51c9038c


CellAssist analysesprovideoptimization,yieldenhancement,andcostsavings

Problem:(a)Thecoatingofplatescanprofoundlyaffectthesurvivabilityandgrowthofcolonies(inthiscasegeneeditedonesstartingwithverysmallquantities). (b)Somecoatingsarealsosignificantlymoreexpensive.

Objectives:Comparetwocoatingstochoosetheoptimalonebycharacterizingandcomparingthecellsunderbothconditionsovertime.(b)Tailor,testanddirectlycomparetheimpactsofnewprotocolstoselectthebestcoatingANDprotocol.

CellAssistSolution:TheCellAssist’s documentation,colonycountingcurvesandexaminationofimagesareusedtocharacterizethenumberofcoloniesforeachcondition,andtheresultsarecollatedandeasilycomparedacrossconditions.

CustomerApplication:ComparisonofCellGrowthonVariousPlateSurfaceCoatings


CustomerApplication:Time-StampedBarcodeDocumentationforReproducibleCellCulture

CellAssist automaticallytrackscells,metrics,platesandreagentsthroughoutthelifecycleofaproject


Analytics.Automation.BetterBiology.

Contact:ThomasForestFarb-Horch,CEO

[email protected]

ThriveBioscience,Inc.,11AudubonRoad,Wakefield,MA01880USA

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