thermo scientifi c lc accessories lc columns and...
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Viper Fingertight FittingsProvides ease of use and dead-volume free plumbing of every conventional HPLC and UHPLC system
• Provides virtually zero-dead volume fi ngertight connections
• Supports operating pressures up to 1250 bar (18,130 psi)
• Available in different lengths: 65mm and from 150 to 950mm in 100mm steps
• Available in different inner diameters: 0.1mm (0.004in), 0.13mm (0.005in) or 0.18mm (0.007in)
• Easy to use due to stainless steel or biocompatible MP35N™ capillaries (1/32in OD) and fi ngertight design
• Works with virtually any valve and column from any manufacturer
• Fits narrow connections such as 10-port valves and enables mixed use with different designs
The Thermo Scientific™ Dionex™ Viper™ fingertight fitting system provides ease of use and virtually dead-volume free plumbing of every conventional HPLC and modern UHPLC system. Together with flexible stainless steel (SST) or biocompatible MP35N® capillaries, it opens a new dimension in liquid chromatography. The Viper system improves chromatographic results, independent of various different connection geometries and system backpressures. Connecting LC modules,
valves, and columns quickly and easily without tools is simple with the Viper system.
Extra column volumes in HPLC have the most detrimental effects on the separation efficiency of an LC system and must be minimized. Conventional fittings tightened by hand or using tools have considerable drawbacks which can compromise efficiency. The Viper fitting system overcomes these
drawbacks by design, working without ferrules to reduce the dead volume of any fluidic connection to virtually zero. The Viper system unifies robust performance, ease of use, acceptable lifetime, and universal compatibility with virtually all different valves and columns for HPLC system users. All Thermo Scientific™ Dionex™ UltiMate™ 3000 XRS, RS, BioRS and SD systems are equipped with Viper fingertight fitting system as a standard.
Thermo Scientifi c LC Accessories
Viper Accessories
Description Cat. No. Quantity
Plug, SST 6040.2303 1 Each
Union, SST 6040.2304 1 Each
Viper Fingertight Fitting Systems – SST and Biocompatible MP35N
Length (mm) 0.1mm ID, SST 0.1mm ID, MP35N 0.13mm ID, SST 0.18mm ID, SST 0.18mm ID, MP35N
65 6040.2207 6042.2306 6040.2307 6040.2357 –
150 6040.2215 6042.2320 6040.2315 6040.2360 –
250 6040.2225 6042.2330 6040.2325 6040.2385 6042.2315
350 6040.2235 6042.2340 6040.2335 6040.2375 6042.2327
450 6040.2245 6042.2350 6040.2345 6040.2365 6042.2337
550 6040.2255 6042.2360 6040.2305 6040.2355 6042.2365
650 6040.2265 – 6040.2310 6040.2395 –
750 – – 6040.2320 6040.2370 6042.2355
850 – – 6040.2330 6040.2380 –
950 – – 6040.2350 6040.2390 6042.2375
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Viper Fingertight Fitting Kits for UltiMate 3000 systems
Description SD Systems RS Systems
Viper Capillary Kit for ISO, LPG or DGP pumps 6040.2302 6040.2301
Viper Capillary Kit for HPG pumps 6040.2309 6040.2308
Viper Capillary Kit for biocompatible RSLC systems – 6841.2301
On-line SPE Solution Kit for x2 Dual Standard or RSLC systems 6040.2802 6040.2801
Tandem Operation Solution Kit for x2 Dual Standard or RSLC systems 6040.2804 6040.2803
Application Switching Solution Kit for x2 Dual Standard or RSLC systems 6040.2806 6040.2805
Parallel Setup Solution Kit for x2 Dual Standard or RSLC systems 6040.2810 6040.2809
Inverse Gradient Kit for x2 Dual Standard or RSLC systems 6040.2819 6040.2820
Automated Method Scouting Solution Kit for Standard or RSLC systems 6040.2808 6040.2807
MS Connection Kit for MS-frontends with WPS autosampler, excluding UV detection 6720.0355 6720.0370
MS Connection Kit for MS-frontends with WPS autosampler, including UV detection 6720.0365 6720.0375
MS Connection Kit for MS-frontends with HPG-RS pump and OAS autosampler excluding UV detection – 6720.0372
MS Connection Kit for MS-frontends with HPG-RS pump and OAS autosampler including UV detection – 6720.0377
Description XRS Systems
Viper Capillary Kit for standalone system with with WPS autosampler 6043.2301
Viper Capillary Kit for standalone system with with OAS autosampler 6845.2301A
MS Connection Kit for MS-frontends with WPS autosampler excluding UV detection 6720.0380
MS Connection Kit for MS-frontends with WPS autosampler including UV detection 6720.0385
MS Connection Kit for MS-frontends with LPG-XRS pump and OAS autosampler excluding UV detection 6720.0372
MS Connection Kit for MS-frontends with LPG-XRS pump and OAS autosampler including UV detection 6720.0377
Viper Fingertight Fittings Kits for UltiMate 3000 Systems
An overview of all UltiMate 3000 systems can be found in the Instruments spare parts section on 4-209.
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nanoViper Fingertight Fittings The Thermo Scientific™ Dionex™ nanoViper™ fingertight UHPLC fitting system is virtually dead volume-free by design. It offers nano LC connections that never fail and brings a peace of mind appreciated by novices, as well as the most experienced users.
• Provides virtually zero-dead-volume connections
• Compatible with backpressures up to 1000 bar (14,500 psi)
• Suitable for temperatures up to 80°C
• Easy to use 1/32" PEEK sheathed fused silica and fi ngertight design
• Works with virtually any valve, and any column, from any manufacturer
• Paves the way for the easy, tool-free nano, capillary and micro LC setup of any application
• nanoViper fi ngertight fi ttings allow you to confi gure your nano, capillary, and micro LC application completely tool free.
Classical connections in nano LC involve either PEEKsil™ or fused silica in PEEK™ sleeves. These connections consist of a nut, ferrule, sleeve, and fused silica, which all need to be assembled correctly. The sealing is at the ferrule, which theoretically leaves a large potential dead volume. Apart from the risk of dead volume (an extreme case indicated above), there is also a risk of damaging the fused silica tubing or column at the point where the ferrule is compressed. The ferrule has to hold the tubing in the sleeve and seal of the connection.
The nanoViper connections come preassembled and high pressure tested. The fittings do not “grab” the tubing at a single point. It seals at the tip and not with a ferrule. Nor does it “grab” the tubing at a single point. It is this feature that gives nanoViper fittings their UHPLC, virtually dead-volume-free capabilities. The theoretical performance effect between the two example chromatograms below is about 20% increase in peak capacity.
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nanoViper Fingertight Fittings
Length (mm) Orange20μm ID
Brown50μm ID
Black75μm ID
Red100μm ID
Purple150μm ID
70 6041.5120 6041.5123 6041.5126 6041.5820 6041.5817
150 6041.5121 6041.5124 6041.5127 6041.5811 6041.5818
250 – – 6041.5730 6041.5812 6041.5819
350 6041.5240 6041.5540 6041.5735 6041.5813 6041.5820
450 – – – 6041.5814 6041.5821
550 6041.5260 6041.5560 6041.5760 6041.5815 6041.5822
650 6041.5275 6041.5575 6041.5775 – –
750 6041.5280 6041.5580 6041.5780 6041.5816 6041.5823
950 6041.5122 6041.5125 6041.5128 – –
Trap Column Cartridges Holders with nanoViper Fittings
Description Cartridge Length (mm) Fitting ID (μm) Cat. No.
μ-Precolumn holder for trap cartridges with 2 x 100mm length nanoViper fi ttings
5 30 164649
15 75 164650
Sample Loops with nanoViper Fittings
Volume (μL) Cat. No. Quantity
1 6826.2401 1 Each
5 6826.2405 1 Each
10 6826.2410 1 Each
20 6826.2420 1 Each
50 6826.2450 1 Each
125 6826.2412 1 Each
nanoViper Fitting Kits for UltiMate 3000 RSLCnano Systems
Description Tubing Samples Trap Column Separation Column RSLCnano Systems
Direct Injection nano LC Kit Y Y – Y 6720.0300
Direct Injection capillary LC Kit Y Y – Y 6720.0305
Preconcentration nano LC kit Y Y Y Y 6720.0310
Preconcentration capillary LC kit Y Y Y Y 6720.0315
Preconcentration monolithic LC kit Y Y Y Y 6720.0320
2D salt plugs kit Y Y Y Y 6720.0325
Automated off line SCX-RP peptides kit Y Y Y Y 6720.0330
Automated off line RP-RP peptides kit Y Y Y Y 6720.0340
Tandem nano LC kit Y Y Y Y 6720.0335
FLM nanoViper tubing kit (All tubing for nano LC preconcentration)
Y – – – 6041.5100
MS connection kit Y – – – 6720.0345
EASY-Spray connection kit (Supports direct injection as well as preconcetration)
Y Y Y – 6720.0395
nanoViper Application KitsnanoViper fittings are also included in tubing and application kits designed for use with the UltiMate 3000 RSLCnano systems.
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SLIPFREE Connectors Universal self-adjusting connections
• Void-free and leak-free by pushing tubing and ferrule into the end-fi tting
• Compatible with all column end-fi ttings
• Stainless-steel threads
• Fingertight connections to 10,000psi
SLIPFREE Connectors, Single
Length (cm) 0.005in ID 0.010in ID 0.020in ID
Single6 30106 31106 3210610 30110 31110 3211020 30120 31120 3212030 30130 31130 3213050 30150 – –
Single Flexible10.5 30111-FLEX 39111-FLEX –15 30115-FLEX 39115-FLEX –28 30128-FLEX 39128-FLEX –40 30140-FLEX 39140-FLEX –50 30150-FLEX – –60 30160-FLEX – –
Single PEEK Collared6 30306 31306 3230610 30310 31310 3231020 30320 31320 32320
Single Long-neck10 30510 31510 –20 30520 31520 32520
SLIPFREE Connectors, Double
Length (cm) 0.005in ID 0.010in ID 0.020in ID
Double6 30206 31206 3220610 30210 31210 3221020 30220 31220 3222030 30230 31230 3223040 30240 – –
Double Flexible10.5 30211-FLEX 39211-FLEX –15 30215-FLEX 39215-FLEX –28 30228-FLEX 39228-FLEX –40 30240-FLEX 39240-FLEX –
Double PEEK Collared6 30406 31406 3240610 30410 31410 3241020 30420 31420 32420
Double Long-neck10 31710 32710 –20 31720 32720 –
SLIPFREE Ferrules
Material Cat. No. Quantity
PEEK 36023 1 Each
Vespel 36024 1 Each
Kel-F 36025 1 Each
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PTFE One-Piece Column Connector Excellent for high-throughput screening and quick connection
• Fingertight, leak-free connection of analytical and guard columns with 10-32 threads
• Minimizes dead volume
• Inert and biocompatible material
Solvent Inlet Filters Feature a large surface area for a long lifetime
• 10μm inlet fi lters for longer lifetime
• No tools required for replacement
Solvent Filters for HPLC Systems
Type For Use with Cat. No. Quantity
Stainless Steel Fit 1/16in OD tube to 1/8in OD plastic tubing A-302 1 Each
Stainless Steel Fit to 1/8in OD plastic tubing using 1/8in PP nut A-302A 1 Each
Bottom-of-the-Bottle 3/16in OD plastic tubing A-436 1 Each
Bottom-of-the-Bottle 1/8in OD tubing A-437 1 Each
PEEK One-Piece Column Connector
Description Cat. No. Quantity
One Piece Coupler 60170-370 1 Each
Solvent Inlet Filters for UltiMate 3000 Systems
Description Model Cat. No. Quantity
Filter frit, porosity 10μm, V4A stainless steel
UltiMate 3000 pumps 6268.0110 10 Each
Filter frit, porosity 10μm, titanium UltiMate 3000 pumps 6268.0111 10 Each
Filter holder UltiMate 3000 pumps 6268.0115 6 Each
Solvent supply line, 1.5 x 3.0 x 1000mm
UltiMate 3000 pumps 6030.2548 1 Each
Bottom-of-the-Bottle solvent fi lters• Effi cient draw
• 100% PTFE polymer, including 2μm fi lters
• Built-in helium sparge port and frit
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High Pressure Stainless Steel Nuts and Ferrules Accommodate a wide range of configurations
• Designed for 10-32 port confi gurations
• Burr and contaminant free
Reducing Union for Preparative Columns Connects 30 to 50mm ID preparative columns to 1/16in tubing
• Stainless steel construction
• 1.0mm bore
• Without frit
Reducing Union for Preparative Column
Description Cat. No. Quantity
1/8in to 1/16in Reducing Union for Preparative Column 60182-357 1 Each
Thermo Scientifi c High Pressure Stainless Steel Nuts and Ferrules
Type Cat. No. Quantity
10-32 thread nut with ferrule F-190 1 Each
Replacement PEEK Ferrules F-192x 10 Pack
Male hex nut U-400x 10 Pack
Universal ferrules, 0.625in U-401x 10 Pack
Valco male hex nut, 10-32 thread U-320x 10 Pack
Valco ferrules, 0.625in U-321x 10 Pack
Male hex nut, Waters compatible U-410x 10 Pack
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PEEK Fingertight Fittings Machined for reliability and ease of use
• Resist cracking, breaking, thread stripping and leaking in both low and high pressure applications
• Biocompatible for a broad range of applications
Stainless Steel Unions, Tees and Crosses Well-suited to high pressure applications
• Absolute zero or low dead volume formats
• Includes two stainless steel nuts and ferrules
Stainless Steel Unions, Tees and Crosses
Description Through Hole (in) Swept Volume (μL) Cat. No. Quantity
Union, stainless steel, Upchurch Scientifi c/Parker fi ttings compatible, includes 2 stainless steel nuts and ferrules 0.010 0.025 U-435 1 Each
Union, stainless steel, Upchurch Scientifi c/Parker fi ttings compatible, includes 2 stainless steel nuts and ferrules 0.020 0.134 U-402 1 Each
Union, stainless steel, Upchurch Scientifi c/Parker fi ttings compatible, includes 2 stainless steel nuts and ferrules 0.050 0.836 U-437 1 Each
Union, stainless steel, Upchurch Scientifi c/Parker fi ttings compatible, includes 2 stainless steel nuts and ferrules 0.062 ~0.0 U-438 1 Each
Union, stainless steel, Waters fi ttings compatible, includes 2 stainless steel nuts and ferrules 0.020 0.129 U-412 1 Each
Union, stainless steel, Valco fi ttings compatible, includes 2 stainless steel nuts and ferrules 0.020 0.103 U-322 1 Each
Tee, stainless steel, 10-32 fi ttings for use with 1/16in OD tubing 0.020 0.57 U-428 1 Each
Cross, stainless steel, 10-32 fi ttings for use with 1/16in OD tubing 0.020 0.72 U-430 1 Each
Description Cat. No. Quantity
Viper union, stainless steel 6040.2304 1 Each
Viper UnionsSuitable only for direct connection of two Viper capillaries
PEEK Fingertight Fittings
Type Cat. No. Quantity
One-piece Fingertight Fitting, 1/16in, 0.37in head F-120x 10 Pack
One-Piece Long Fingertight Fitting, 1/16in, 0.37in head F-130x 10 Pack
One-Piece PEEK Fingertight Fitting, 1/32in, 0.25in head M-645x 10 Pack
Two-Piece Fingertight Wing Nut with Ferrule, 1/16in F-300x 10 Pack
Replacement PEEK Ferrules F-142x 10 Pack
Column End Plugs, 1/16in, 10-32 coned, Delrin, Black U-467BLKx 10 Pack
Column End Plugs, 1/16in, 10-32 coned, Delrin, Red U-467Rx 10 Pack
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PEEK Unions, Tees and Crosses Well-suited to high pressure applications
• Absolute zero or low dead volume formats
• Biocompatible
PEEK and PEEK Lined Unions, Tees and Crosses
Description Through Hole (in) Swept Volume (μL) Cat. No. Quantity
Union, PEEK polymer, includes two PEEK 2-piece fi ttings 0.010 0.070 P-742 1 Each
Union, PEEK polymer, includes two PEEK 2-piece fi ttings 0.020 0.28 P-704 1 Each
Tee, PEEK, 10-32 fi ttings for use with 1/16in OD tubing, includes three 10-32 PEEK double-winged nuts 0.020 0.57 P-727 1 Each
PEEK, 10-32 fi ttings for use with 1/16in OD tubing, includes four 10-32 PEEK double-winged nuts 0.020 0.72 P-729 1 Each
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PEEKsil Capillary Tubing Excellent chemical compatibility and very low carryover
• Precision-bore fused silica tubing coated with 1/16in OD PEEK covering
• Usable in most standard chromatography systems
• Withstands high pressures
• Smooth internal surface for excellent fl ow characteristics
• Tubing is stiff: not recommended for uses requiring tubing bends
• Precut lengths only: cutting in the lab may damage tubing
Applications:
• HPLC
• LC-MS
PEEK Sleeves for Fused Silica Capillary Tubing Withstands high pressures
PEEKsil Capillary Tubing
ID (in) Length (cm) Cat. No. Quantity
0.002 10 60182-500 5 Pack
20 60182-501 5 Pack
50 60182-502 2 Pack
0.004 10 60182-503 5 Pack
20 60182-504 5 Pack
50 60182-505 2 Pack
0.007 10 60182-506 5 Pack
20 60182-507 5 Pack
50 60182-508 2 Pack
1/16in OD PEEK Sleeves for Fused Silica Capillary Tubing
ID (in) Color Cat. No. Quantity
0.008 Yellow F-227 1 Each
0.010 Blue F-228 1 Each
0.012 Natural F-229 1 Each
0.015 Orange F-230 1 Each
0.021 Natural F-231 1 Each
0.030 Natural F-232 1 Each
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316 Stainless Steel Capillary Tubing Cleaned, polished, passivated and ready-to-use
• Suitable for ultra high pressure applications
• Wide chemical compatibilty
• Prefi nished, square, burr-free ends and interiors to minimize dead volume connections
• Not recommended for biological samples
• Rough internal surface may lead to sample carryover
316 Stainless Steel Capillary Tubing
ID (in) Length (cm) Color Cat. No. Quantity
1/16in OD Precut Tubing
0.005 5 Red U-152 1 Each
10 Red U-153 1 Each
20 Red U-154 1 Each
30 Red U-155 1 Each
50 Red U-156 1 Each
100 Red U-157 1 Each
0.007 5 Black U-126 1 Each
10 Black U-127 1 Each
20 Black U-128 1 Each
30 Black U-129 1 Each
50 Black U-130 1 Each
100 Black U-131 1 Each
0.010 5 Blue U-111 1 Each
10 Blue U-112 1 Each
20 Blue U-113 1 Each
30 Blue U-114 1 Each
50 Blue U-132 1 Each
100 Blue U-133 1 Each
1/32in OD Precut Tubing with 1/16in Sleeves
0.005 10.5 Red 30011-FLEX 1 Each
15 Red 30015-FLEX 1 Each
28 Red 30028-FLEX 1 Each
40 Red 30040-FLEX 1 Each
0.007 10.5 Yellow 39011-FLEX 1 Each
15 Yellow 39015-FLEX 1 Each
28 Yellow 39028-FLEX 1 Each
40 Yellow 39040-FLEX 1 Each
1/16in 316 Stainless Steel Tubing, 5-Foot Coil
ID (in) Cat. No. Quantity
0.005 U-158 1 Each
0.007 U-108 1 Each
0.010 U-106 1 Each
0.020 U-105 1 Each
0.030 U-107 1 Each
0.040 U-144 1 Each
0.046 U-151 1 Each
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PEEK Capillary Tubing Pre-cut and color-coded for easy identification and use
• Broad chemical compatibility
• Biocompatible
• Easily cut to desired length
• Appropriate for many HPLC applications
• Resistant to most organic solvents, but nitric acid, sulfuric acid, dichloromethane, THF and DMSO are not recommended
1/16in OD Precut PEEK Tubing
ID (in) Length (cm) Color Cat. No. Quantity
0.003 5 Natural 37003-5 1 Each
10 Natural 37003-10 1 Each
20 Natural 37003-20 1 Each
30 Natural 37003-30 1 Each
50 Natural 37003-50 1 Each
100 Natural 37003-100 1 Each
0.005 5 Red 37005-5 1 Each
10 Red 37005-10 1 Each
20 Red 37005-20 1 Each
30 Red 37005-30 1 Each
50 Red 37005-50 1 Each
100 Red 37005-100 1 Each
0.007 5 Yellow 37007-5 1 Each
10 Yellow 37007-10 1 Each
20 Yellow 37007-20 1 Each
30 Yellow 37007-30 1 Each
50 Yellow 37007-50 1 Each
100 Yellow 37007-100 1 Each
0.010 5 Blue 37010-5 1 Each
10 Blue 37010-10 1 Each
20 Blue 37010-20 1 Each
30 Blue 37010-30 1 Each
50 Blue 37010-50 1 Each
100 Blue 37010-100 1 Each
0.020 5 Orange 37020-5 1 Each
10 Orange 37020-10 1 Each
20 Orange 37020-20 1 Each
30 Orange 37020-30 1 Each
50 Orange 37020-50 1 Each
100 Orange 37020-100 1 Each
1/16in OD PEEK Tubing, 5-Foot Coil
ID (in) Cat. No. Quantity
0.003 37003 1 Each
0.005 37005 1 Each
0.007 37007 1 Each
0.010 37010 1 Each
0.020 37020 1 Each
0.030 37030 1 Each
0.040 37040 1 Each
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Polymer Tubing Cutter Produces a flat, 90°, burr-free end
• Compatible with rigid polymeric tubing
• Guide holes for 1/16in and 1/8in tubing
Terry Tool Tubing Cutters Produce clean, 90° cuts of stainless steel tubing
Fused Silica Cutter
Polymer Tubing Cutter
Description Cat. No. Quantity
Polymeric Tubing Cutter A-327 1 Each
Replacement blades A-328 5 Pack
Terry Tool Tubing Cutters
Description Cat. No. Quantity
1/16in stainless steel tubing 60182-509 1 Each
1/8in stainless steel tubing 60182-510 1 Each
Fused Silica Cutter
Description Cat. No. Quantity
Cutter for fused silica capillaries 6720.0016 1 Each