the antibacterial powers of honey elliot young. honey
TRANSCRIPT
The antibacterial powers of honey
Elliot Young
Honey
Background
Honey’s antibacterial properties
High Sugar Concentration
MethylglyoxalHydrogen Peroxide
Background
Honey’s antibacterial properties
High Sugar Concentration
MethylglyoxalHydrogen Peroxide
Background
Honey’s antibacterial properties
High Sugar Concentration
MethylglyoxalHydrogen Peroxide
Background
Background
+
Background
Honey’s antibacterial properties
High Sugar Concentration
MethylglyoxalHydrogen Peroxide
Methodology• 3 honeys compared:
• Processed• Unprocessed• 10+ UMF Manuka
• Honey’s antibacterial qualities tested against escherichia coli and bacillus subtilis
Hypothesis: As I increase the concentration of each honey, the antibacterial effect of the honey will increase (reflected by the diameter of clear zone). Manuka honey should also have a stronger antibacterial quality compared to the other honeys due to the significant levels of methylglyoxal (MGO) in the manuka.
Methodology
Clear zone diameters measured & recorded
Agar plates incubated for 42 hours at 25oC
5 different concentrations of honey plated
0.09ml honey sample placed in each hole
Four holes taken out of each agar plate with a sterile cork borer
Agar plates inoculated with 0.5ml bacteria
Results & Analysis
0 10 20 30 40 50 60 70 80 90 1000
5
10
15
20
25
concentration of honey /%
Mea
n di
amet
er o
f cle
ar z
one
/mm
0 10 20 30 40 50 60 70 80 90 1000
5
10
15
20
25
Processed HoneyLinear (Processed Honey)Unprocessed Honey
concentration of honey /%
Mea
n di
amet
er o
f cle
ar z
one
/mm
Honeys antibacterial qualities against two bacteria
Escherichia coli Bacillus subtilis
r>0.8All 3 honeys had antibacterial properties
Results & AnalysisHoneys antibacterial qualities at 100%
Concentration against two bacteria
Escherichia coli Bacillus subtilis
0
5
10
15
20
25
Honey Type
Dia
met
er o
f cle
ar
zone
/m
m0
5
10
15
20
25
Honey Type
Dia
met
er o
f cle
ar
zone
/m
m
Processed Unprocessed Manuka Processed Unprocessed Manuka
10+ UMF manuka honey had over twice the antibacterial effect as processed honey at 100% concentration
Difference between processed and unprocessed relatively insignificant
Applications & Further Research
Applications
Antibiotic Resistant Bacterial
Strains
Throat Infections
UlcersFood
Poisoning
Wound Patches
