testing on soybean - ista · 4th ista proficiency test on gmo testing on soybean ista proficiency...

4th ISTA Proficiency Test on GMO Testing on Soybean

ISTA Proficiency Working Groupof the GMO TF

B. Kahlert, S. Grégoire, C. Haldemann

ISTA Ordinary Meeting – Results of the ISTA GMO Proificiency Tests

Outline

• IntroductionDesign of the 4th Proficiency TestWhat is new in this test round?

• ResultsQualitative TestSub-sampling QuantificationQuantitative Test

• Summary


Design of the 4th Proficiency Test

• crop: Glycine max (L.) Merr.• # of samples per lab: 12• # of negative samples: 3• # of positive samples: 9

0.1%: 30.5%: 31.0%: 3

• event: GTS-40-3-2• sample size: 3000 seeds• qualitative, sub-sampling quantification and

quantitative test


What was new in this test round?

• crop: soybean• event was known by the lab• information about source of reference material was

provided• seeds for each sample were counted• conventional seed and GM seed part were weighted • Qualitative and quantitative test were obligatory


Submitted Test Results

qual. & sub-sampl.

11 (18%)

qual., sub-sampl. & quant.

1 (2%)

qual.3 (5%)

no results9 (15%)

qual. & quant.36 (60%)


Performed tests by the laboratories...

PT01 PT02 PT03 PT04no results 11% 10% 17% 15%qual. 89% 29% 23% 5%qual. & sub-sampl. - 23% 15% 18%qual. & quant. - 30% 45% 60%qual. & sub-sampl. & quant. - 8% 0% 2%

Total # of laboratories 43 52 48 60

Qualitative Test

Qualitative Result:

• The detection of GM seeds in conventional seed lots.

• The laboratory classified each of the 12 samples as either positive or negative.

ISTA Ordinary Meeting – Results of the ISTA GMO Proficiency Tests

Qualitative Test

correct results42 (82%)

false results9 (18%) false negative

results5 (10%)

false positive results3 (6%)

both false neg. and

pos. results 1 (2%)


Laboratories which reported...

PT01 PT02 PT03 PT04Correct results 70% 85% 81% 82%False results 30% 15% 19% 18%False negative 19% 4% 12% 12%False positive 7% 4% 5% 8%Both false pos & neg 5% 6% 2% 2%

Qualitative Test

153

153

153

459

153

612

3

0

7

10

8

18

0% 20% 40% 60% 80% 100%

1.0% GM seeds

0.5% GM seeds

0.1% GM seeds

Positive samples

Negative samples

All samples

% of samples tested# of samples reported as false

2.9%5.2%2.2%4.6%0.0%2.0%


Samples classified as...

PT01 PT02 PT03 PT04Correctly reported results 95.4% 96.4% 95.9% 97.1%False negative 6.5% 3.0% 4.3% 2.2%False positive 1.7% 5.0% 3.3% 5.2%

Sub-sampling Quantification

The laboratory reported the test plan...• the size of sub-samples (in number of seeds)• the number of sub-samples tested• the number of positive sub-samples

and the results...• whether the % of GM seeds is ≤ 1% or >1%• the estimate of the percentage of GM seeds per

sample (in number of seeds)



Tools: Seedcalc6 and % gm estimate.xls


Sub-sampling QuantificationISTA Ordinary Meeting – Results of the ISTA GMO Proficiency Tests

Laboratory’s estimated results and its mean

Labs# of sub-samples

size ofsub-sample

(1), 2, 3, 4 30 1005, 6 20 150

7 15 2008, 9 10 300

10 6 50011 2 to 6 100 to 300

Estim

ate

of %

GM

see

ds

0

0.2

0.4

0.6

0.8

1

1.2

1.4

lab1

lab2

lab3

lab4

lab5

lab6

lab7

lab8

lab9

lab1

0

lab1

1

0

0.2

0.4

0.6

0.8

1

1.2

1.4

lab1

lab2

lab3

lab4

lab5

lab6

lab7

lab8

lab9

lab1

0

lab1

1

0

0.2

0.4

0.6

0.8

1

1.2

1.4

lab1

lab2

lab3

lab4

lab5

lab6

lab7

lab8

lab9

lab1

0

lab1

1

Spiking level 0.1% Spiking level 0.5% Spiking level 1.0%


Spiking level

0.1% 0.5% 1.0%

Test

resu

lts (%

)

0.0

0.2

0.4

0.6

0.8

1.0

1.2

1.4


and PT03 (5 labs)

Spiking level

0.2% 2.0% 4.0%

Test

resu

lts (%

)0.0

1.0

2.0

3.0

4.0

5.0

Results of PT04 (11 labs)

Quantitative Test

Quantitative Result:

• The laboratory reported the GM content of thepositive test samples.

• The result should be given in % of GM seed by mass or % seed by number.

• The result could be given in another unit chosen bythe laboratory.

36 laboratories reported in 39 test series


Quantitative TestISTA Ordinary Meeting – Results of the ISTA GMO Proficiency Tests

Protocol of Results


Quantitative Test

Spiking level 0.1%

0.0

0.2

0.4

0.6

0.8

1.0

all

lab1

2

lab1

3

lab1

4

lab1

5

lab1

6

lab1

7

lab1

8

lab1

9

lab2

0

lab2

1

lab2

2

lab2

3

lab2

4

lab2

5

lab2

6

lab2

7

lab2

8

lab2

9

lab3

0

lab3

1

lab3

2

lab3

3

lab3

4

lab3

5

lab3

6a

lab3

7

lab3

8

lab3

9

lab4

0

lab4

1

lab4

2

lab4

3a

lab4

4

lab4

5

lab4

6

lab4

7

lab3

6b

lab4

3b

lab4

8

Mea

n pe

rcen

tage

GM

see

ds +

/- SD

(%)

NB : laboratory number has been re-coded


Quantitative Test

Spiking level 0.5%

0.0

0.2

0.4

0.6

0.8

1.0

1.2

1.4

1.6

1.8

2.0

2.2

2.4

all

lab2

6

lab1

2

lab1

3

lab2

2

lab2

1

lab1

4

lab2

3

lab2

4

lab4

2

lab4

7

lab2

9

lab1

5

lab3

0

lab2

7

lab1

6

lab3

6a

lab3

1

lab1

7

lab1

8

lab3

9

lab3

4

lab3

3

lab2

8

lab1

9

lab4

1

lab4

0

lab3

5

lab4

6

lab2

5

lab3

7

lab3

8

lab3

6b

lab4

8

lab2

0

lab4

4

lab4

3b

lab3

2

lab4

5

lab4

3a

Mea

n pe

rcen

tage

GM

see

ds +

/- SD

(%)


Quantitative Test

Spiking level 1.0%

0.00.20.40.60.81.01.21.41.61.82.02.22.42.62.83.03.23.43.63.84.04.2

all

lab1

2

lab1

3

lab2

6

lab2

1

lab3

2

lab2

3

lab2

2

lab1

5

lab4

2

lab2

4

lab4

7

lab2

8

lab1

7

lab3

0

lab1

4

lab3

6a

lab2

5

lab3

3

lab3

9

lab3

5

lab1

9

lab2

0

lab1

6

lab3

7

lab4

1

lab3

8

lab2

7

lab4

0

lab3

1

lab1

8

lab4

4

lab4

8

lab4

6

lab3

6b

lab4

5

lab2

9

lab3

4

lab4

3b

lab4

3a

Mea

n pe

rcen

tage

GM

see

ds +

/- SD

(%)


Quantitative Test

0.0

0.5

1.0

1.5

2.0

2.5

3.0

lab1

2

lab1

3

lab1

4

lab1

5

lab1

6

lab1

7

lab1

8

lab1

9

lab2

0

lab2

1

lab2

2

lab2

3

lab2

4

lab2

5

lab2

6

lab2

7

lab2

8

lab2

9

lab3

0

lab3

1

lab3

2

lab3

3

lab3

4

lab3

5

lab3

6a

lab3

6b

lab3

7

lab3

8

lab3

9

lab4

0

lab4

1

lab4

2

lab4

3a

lab4

3b

lab4

4

lab4

5

lab4

6

lab4

7

lab4

8

0.1% 0.5% 1.0%

Mean per GMO spiking level and laboratory

Results of PT04

TestSpiking level (%) Mean (%)

Standarddeviation

Variationcoeff.

PT04 0.1 0.13 0.11 87%PT04 0.5 0.47 0.29 61%PT04 1.0 0.87 0.32 37%PT03 0.2 0.19 0.10 51%PT03 2.0 1.62 0.92 57%PT03 4.0 3.37 1.68 50%PT02 0.7 0.73 0.37 50%PT02 1.4 1.47 0.91 62%

Spiking level

0.1% 0.5% 1.0%

Test

resu

lts (%

)

0.0

0.5

1.0

1.5

2.0

2.5

3.0


Quantitative Test


Summary/ previous testsPT04 - Qualitative Results:• The percentage of laboratories reporting correct

results for all samples is similar to the previous tests• >80% of labs made no missclassification, • >95% of samples are reported correctlyPT04 - Quantitative Results:• The results show variation increasing along with the

spiking level as in other tests.• A higher number of laboratories reported results that

were close to the true value (spiking level). Theinformation about event and the availability of reference material might have helped the laboratoriesto achieve better results in this round.


5th ISTA Proficiency Teston GMO Testing

• Crop: Soybean• 12 samples of 3000 seeds• Qualitative Test• Sub-sampling quantification or quantitative test• Material Transfer Agreement

• Register now!


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testing on soybean - ista · 4th ista proficiency test on gmo testing on soybean ista proficiency...

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