tenrnessee valley authority. post office box 2000, soddy-daisy ... · dhee0n orupeision h ardieh...

Tenrnessee Valley Authority. Post Office Box 2000, Soddy-Daisy, Temessee 37379-2000

August 12, 2004

State of TennesseeDepartment of Environment and ConservationDivision of Water Pollution ControlEnforcement & Compliance Section6e Floor, L & C Annex401 Church StreetNashville, Tennessee 37243-1534

Attention: Mr. Chip Hannah

Dear Mr. Hannah:

SEQUOYAH NUCLEAR PLANT - DISCHARGE MONITORING REPORT FORJULY 2004

Enclosed is the July 2004 Discharge Monitoring Report for Sequoyah Nuclear Plant.Please contact me at (423) 843-6700 if you have any questions or comments.

Sincerely,

)kd&k.aWcSStephanie A. HowardPrincipal Environmental EngineerSignatory Authority forJ. Randy DouetSite Vice PresidentSequoyah Nuclear Plant

Enclosurecc (Enclosure):

Chattanooga Environmental Assistance CenterDivision of Water Pollution ControlState Office Building, Suite 550540 McCallie AvenueChattanooga, Tennessee 37402-2013

U.S. Nuclear Regulatory CommissionATTN: Document Control DeskWashington, D.C. 20555

PERMITTEENAMEIADDRESS ncude FecafftV NamAAottn ifDffDnOName TWA - SEQUOYAH NUCLEAR PLANTAddress P.O. BOX 2000__ ___ _ _L -fQ_%0)L0& - - - -n2e - - - - - - -

fac&_ TV -SUOAHgNUCODLEA ff.____Lgcaon HAMILTOCUM2NTY - - - - - - - - - _

NATIONAL POLLUTANT DISCHARGE ELIINATION SYSTEM (NPDES} MAJORDISCHARGE MONITORING REPORT (DM (SUBR 01)

I TN026450 101 G F - FINAL

I PERMITNUMBER I DISCHARGE NUMBER DIFFUSER DISCHARGE

I __,_MONIRING PIO EFFLUENT

Fonn Approved.OMB No. 2040-0004

YEAR I MO I Fo TIFrom 04 | 07 | 011 To 04 | 07 |31|ATTN: Stephanie A. Howard

NO DISCHARGE DNOTE: Read Insrtueflons blarrm k nm this fm_

PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE_ _ _ _ _ _ _ _ _ __ _ _ _ _ _ _ _ _ _ _ _ _ _ _EX OF TYPE

AVERAGE MAX" UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS

TEMPERATURE, WATER DEG. SAMPLE 30.2 04 0 31/31 MODELDCENTIGRADE MEASUREMENT

0010 Z 0 0 - re DEG. C. 7711"N 901 7KrEINSTREAM MONITORING _ ____ C Ž _ _

TEMPERATURE, WATER DEG. SAMPLE __ a. 42.7 0 31/31 RCORDRCENTIGRADE MEASUREMENT 04

00010 1 0 0 to" 27 pDEG.CEFFLUENTGROSS VALWE _ DEG. C. _ i MPH SAMPLE *.** * 7.3 7.4 12 0 8131 GRAB

EFFLUENTGROSS VALUE _ 9.J7 SU 4MEASUREMENT 1200530 1 0 0 Su*-'MI *7

EFFLUENT GROSS VALUE _ MLtL _ _ mOILIAND OREASE SAMPLE 9***n* _5 <5 9 O 4/31 GRAB

MEASUREMENT 19 0

00550 1 0 0 ElIRt< a-<w½ MOGV a 3/3 COD

EFFLUENT GROSS VALUEFLOW, IN CONDUIT OR THRU SAMPLE a<5 1609 03 4/31 GRATREATMENT PLANT MEASUREMENT

505 0 0 WT*******-,M & .rh~

EFFLUENT GROSS VALUE MG _ _ . _

CHLORINE. TOTAL RESIDUAL SAMPLE <0.016 0.030 1 31/31 GRAB

TREATENT LANTMEASUREMENT

5006 1 0 0 -1 - - 5 -MG-L

EFFLUENT GROSS VALUE N,_ MtL _ Y

NAMlrTLE PRINCIPAL EXECUTIVE OFFICER I CrUfytmup dw Ityoflawlutdocumw trd aN tltantawwe.ed dw my . TELEPHONE DATEdhee0n oruPeIsion h ardieh asyat dsied pt 6 u ut qLid ^pwu _ A

J. Randy Douet p randevaluatfoenlt nted.Based on krquryo tien a or A.SiekePrsin Mm t lobelPrincipal Environmental EngIneer 423 843-8700 04 08 12

Site Vic PresIdent ptlie ln tbsbttb false SIGNATURE OF PRINCIPAL EXECUTIVE

TYPED OR PRINTED S ihe poq d lif ne and n l h r b OFFICER OR AUTHORIZED AGENT AREA MB DAY'.4MMUIY>t 50 MIIU tArL1wv' I Iful LFI AN T VIIULA I JNS (Reftfec aN A dfctef at)

No dosed mode operation. The following Injections occured: 1. PCL-222 (max calc. conc. was 0.058mg/L-limt 0.100mg/L) 2. CL-363 (max. cal. cono. was 0.OO9mg/L4fmlt 0.lOOnmgL) 3. PCL-222/PCL-401 (max. cabc. conc. was 0.O20mg/L-4lrnft 0.100mg/L) 4. H-130M (max calc. conc. was 0.031mglr -L-nft 0.050mg/L) S. H-130M (low detection level analytical method was -0.020mg/LA-irnft 0.O50mg/L))

EPAruf XarmI IRLYe M)v rnoumIa Wuwnfm Hmy DU uses Page I of 2

PERMITTEE NAMEIADDRESS flncude Fad/htvName.caWn fDifferentName TVA . SEQUOYAH NUCLEAR PLANT

dud QP.OX B OO _ _ _ _ _ _W_ n_ _ NEROFQ 2900 .B3@ . . _

____ODDY-YE TN 37384.E!fJ!q TV -SEOUOYAH NJILcEA -f-E- --Locaonj __ECT.NTY CUNT________

ATTN: Stephanie A. Howard

NATIONAL POLLUTANT DISCKARGE ELIMINATION SYSTEM (NPDES) MAJORDISCHARGE MONITORING REPORT (DMRR

(SUBR 01)I TN0026450 101 G F - FINAL

I PERMIT NUMBER [IS G NUMBR | DIFFUSER DISCHARGE

Form Approved.OMB No. 204040004

l| -- D - - |

I u&MnnluM nco DIltfl IFrom TDY YoAR MD

From 1 04 1 07 | 01 ITo 04 | 07 1 31|

EFFLUENT

NO DISCHARGE j ]NOTE. Read hsbuctn befbre comoleiln this fom

PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE________ ________________EX ANLY TYPE

AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS |iALYSIS

TEMPERATURE - C, RATE OF SAMPLE 0.1 62 0 31 /31 CALCTDCHANGE MEASUREMENT

8223 1 0 0 ,77- DEG CEFFLUENTGROSS VALUE -D WHRTEMP. DIFF. BETWEEN SAMP. & SAMPLE .. ** 2.1 0 0 31/31 CALCTDUPSTRM DEG.C MEASUREMENT00016 1 S 0 -DEW. 7 D WCEFFLUENT GROSS VALUE tUIMW7'>uos.BORON, TOTAL SAMPLE <0.2 <0.2 <0.2 19 0 1/31 GRAB

EFFLUENT GROSS VALUE ' _ Illwt ; = 1

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NAMEITLE PRINCIPAL EXECUTIVE OFFICER I C undwir ty of lawt I th doastwriid iltWli wS ipWd uiKS _ I TELEPHONE DATEdxdbnrhhmddbmualh-- edwhhIhyWD ^ W ., tK",. ' d~

J. Randy Douet J0cblraP.w.Ion In W moarduMb i a witudw cmd y to .sy. M f PewSie VRad President _ m m Mu iotn *ft ef n Prlncdpal Environmental EngIneer 423 843-6700 04 08 12

SiteV, sd acm I m Wdn M brP eomftqfl SIGNATURE OF PRINCIPAL EXECUTIVETYPDI O PRidNTnE Mu hd a y p u flmwtydofn arid h 1Iiww h w b OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAYTYPED OR PRINTED i I________________________ CODE____________

COMMENTS AND EXPLANATION OF ANY VIOLATIONS p~ence 1 aacments here

CCW data for July 2004 Is attached.

FPA,-F. vr 110 0C Page 2 o 2- F-- .0- %.- -. 1 rV-nw ruwwa meay UV wem Page 2 of 2

CCW TRENCH

Date/Time Collected Extractable Petroleum Hydrocarbons Analysis Date/Time Analyst Method

07/01/2004 0820 <0.5 mg/L 07/02/2004 1813 CVS EPA 8015B

CCW CHANNEL

Date/Time Collected Extractable Petroleum Hydrocarbons Analysis Date/Time Analyst Method

07/01/2004 0815 <0.5 mg/L 07/02/2004 1736 CVS EPA 8015B

PERMITTEE NAME/ADDRESS (Incdude Feo7itvNam7aFocaton ifDietnfName __A - SEQUOYAH NUCLEAR PLANTAddress P.O. BOX 2000

SODOY - DAISY TN 37384 _______fedjly _ _A-S2U AHNqCLEAR PLANT.LcaL HAMILN OQUN T_


NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM O(PDES) MAJOR Fonn Approved.DISCHARGE MONITORING REPORT (DMRJ (SUBR 01) OMB No. 2040-0004

TN0026450 101T F - FINALPERMIT NUMBER DISCHARGE NUMBER BIOMONITORING FOR OUTFALL 101

a~fLrrfM2L ~ _ _ _ _~ I EFFLUENT1 MW1,41 1 %.-FWII%.7- r- I

From O I DAt1 YEAR MO | NO DISCHARGE DNOTE Reed IehictoM before Complet this hrm

PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE__ _ _ _ _ ___ _ _ _ _ _- EX OF TYPE

AVERAGE MAXIMUIM UNITS MINIMM AVERAGE MAXIMUM UNITS ANALYSIS

IC25 STATRE 7DAY CHR SAMPLE >100 23 0 1/0 COMPOSCERIODAPHNIA MEASUREMENT

TP35 1 0 0 , M-MP-- 043EREN ~EFFLUENT GROSS VALUE. ERNT _ _ zIC25 STATRE 7DAY CHR SAMPLE >100 23 0 110 COMPOSPIMEPHALES MEASUREMENT 23

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who menag Do sn, -th Prsnsdre ndyi ogem ,oS Principal Environmental Engineer 43 8370 0 8 1SKe Vioe President ftcnftN Calwnhr SIGNATURE OF PRINCIPAL EXECUTIVE I

TYPED OR PRINTED i f kc rto;ii I IhYofisN a fond W fr bhig violatins. OFFICER OR AUTHORIZED AGENT AREA I NUMEER YiEAR MO DAY

COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Refrm so effheonwt heme)

Toxicity was sample July 5-10 2004. Report is attached.

_ _ _ME~I

EPA Form 3320-1 (REV 31") Pmvfous editions may be used Page I of I

August 9, 2004

Ruth Ann Hurt, SB 2A-SQN

SEQUOYAH NUCLEAR PLANT (SQN) TOXICITY BIOMONITORING, NPDES PERMITNO. TN0026450, OUTFALL 101, JULY, 2004

Attached are two copies of the subject report for submission to the state of Tennessee and acopy of the report for your records. The report provides results of compliance testing usingfathead minnows and daphnids. Outfall 101 samples collected July 5-10, showed no toxiceffects to fathead minnows or daphnids. The resulting IC2s values for both species were> 100 percent. Exposure of fathead minnows and daphnids to intake samples resulted inno significant differences from controls during this study period.

In addition to the routine compliance test, fathead minnows were also tested in Outfall 101and intake samples which were treated using UV exposure for pathogen removal prior tointroduction of test organisms. Fish pathogens present in intake water have been thesuspected cause of anomalous dose responses and high variability among replicates inprevious toxicity testing at Sequoyah. At the time this study was conducted, mortalitywhich occurred in minnows exposed to routine compliance samples and UV treatedsamples was not sufficient to jeopardize statistical validity.

Call me at (256) 386-2755 if you have any questions or comments following your review of thereport.

Cynthia L. RussellBiologistEnvironmental Engineering Services- WestCEB 3A-M

Attachmentcc (Attachment):

Files, ER&TA, CEB IB-M

SQN July 2004M

TENNESSEE VALLEY AUTUORITYTOXICITY TEST REPORT

INTRODUCTION / EXECUTIVE SUMMARYReport Date: August 9.2004

1. Facility I Discharger: Seguovah Nuclear Plant / TVA

2. County / State: Hamilton / Tennessee

3. NPDES Permit#: TN0026450

4. Type of Facility: Nuclear-Fueled Electric Generating Plant

5. Design Flow (MGD): 3.266

6. Receiving Stream: Tennessee River (TRM 483.6)

7. 1Q10: 2.992.4

8. Outfall Tested: 101

9. Dates Sampled: July 5-10. 2004

10. Average Flow on Days Sampled (MGD): 1596. 1592. 1595. 1591. 1592. 1591

11. Pertinent Site Conditions: H-130M molluscicide was injected from July 6-7, 2004. Thedates and times for the H-130M injection are in the following table. See Appendix B forcomplete additional chemical application information during the sample collectionperiod.

injection .ocation Date/Start Time Date/Ending Time

Raw Cooling Water 07/06/2004 1010 07/07/2004 1009

12. Test Dates: July 7-14. 2004

13. Test Type: Short-term Chronic Definitive

14. Test Species: Fathead Minnows (Pimephales promelas)Daphnids (Ceriodaphnia dubia)

15. Concentrations Tested (%/6): Outfall 101: 10.98. 22.0.43.9. 72.0. 100.0Intake: 100.0

Pimephales promelas: UV treated Outfall 101: 10.98. 22.0. 43.9. 72.0. 100.0TV treated Intake: 100.0

16. Permit Limit Endpoint (%/): Outfall 101: ICMs = 43.9%

17. Test Results: Outfall 101: Pimephalespromelas: IC25> 100%Ceriodaphnia dubia: IC > 100%

UV treated Outfall 101: Pimephalespromelas: ICQ, > 100%

18. Facility Contact: Stehanie Howard Phone #: (423) 843-6713

19. Consulting / Testing Lab: Environmental Testing Solutions. Inc.

20. Lab Contact: Jim Sumner Phone #: (828) 350-9364

21. TVA Contact: Cynthia L. Russell Phone #: (256) 386-2755 j

22. Notes: Outfall 101 samples collected July 5-10, 2004, showed no toxic effects tofathead minnows or daphnids. The resulting IC25 values, for both species, were >100 percent. Exposure of fathead minnows and daphnids to intake samples resultedin no significant differences from controls during this study period.

Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fishpathogens present in intake water have been the suspected cause of interference (anomalousdose response and high variability among replicates) in previous toxicity testing atSequoyah. At the time this study was conducted, insignificant mortality occurred inminnows exposed to non-treated and UV treated samples, suggesting that pathogenicbacteria were not present.

2

METHODS SUMMARY

Samples:

1. Sampling Point: Outfall 101. Intake

2. Sample Type: Composite

3. Sample Information:

Date Date Date(MM/DD[YY)/ (MDD/YY)/ Arrival Initial (MMIDD/YY)/

Sample Time (ET) Time (ET) Temp. TRC* Time (ET)ID Collected Received .. mL Used By

07/05/04 0746 to 07/07/04 143511 07/06/04 0646 070/413 .~ <.0 07/08/04 1409

Intake 07/05/04 0803 to 07/06/04 1338 1.6 <0.10 07/07/04 1435_____ 07/06/04 0703 _________ 07/08/04 1409

101 07/07/04 0737 to 07/08/04 1300 2.4 <010 07/09/04 1400_____ 07/08/04 0637 0/8413007/10/04 1351

Intake 07/07/04 0717 to 07/08/04 1300 2.3 <0.10 07/09/04 1400_____ 07/08/04 0617 ____ __ 07/10/04 1351

07109/04 0740 to 07/11/04 1346101 07/09/04 0740 to 07/10/04 1232 2.4 <0.10 07/12/04 1400

07/10/4064007/13/04 141307/0904 078 to07/11/04 1346

Intakce 07/109/04 0718 07/10/04 1232 3.1 <0.10 07/12/04 140007/1/04 61807/13/04 1413

TRC -Total Residual ChlorinetSamples were collected in two 2.5 gallon cubitainers. Temperature was measured in each cubitainer upon arrival

4. Sample Manipulation: Samples from Outfall 101 and intake were warmed to test temperature(25.0 k 1.0LC) in a warm water bath.

Aliquots of Outfall 101 and Intake samples were UV-treated through a40-watt Smart UV Sterilizer (manufactured by Emperor Aquatics.Inc.! for 2 minutes.

3

Pimephales promelas Cerlodaphnia dulbiaTest Organisms:

1. Source: Aquatic BioSystems. Inc. In-house Cultures

2. Age: 23.5-25.5 hours old <24-hours old

Test Method Summary:

1. Test Conditions: Static. Renewal Static. Renewal

2. Test Duration: 7 days Until at least 60% of controlfemales have 3 broods

Moderately Hard Synthetic3. Control / Dilution Water: Moderately Hard Synthetic

4. Number of Replicates:. 4

5. Organisms per Replicate: 10

10

I

6. Test Initiation: (Date/Time)Outfall 101

IV Treated Outfall 101

7. Test Termination: (Date/Time)Outfall 101

UV Treated Outfall 101

07/07/04 - 1435 ET07/07/04 - 1415 ET

07/14/04 - 1409 ET07/14/04 - 1352 ET

07/07/04 - 1104 ET

07/14/04 - 1023ET

8. Test Temperature: Outfall 101: Mean = 24.80C(24.0 - 25.50C)

Test Temperature: UV-Treated Outfall 101: I

Mean = 24.90C(24.4 - 25.4LC)

Mean = 24.80C'24.0 - 25.50C)

9. Physical / ChemicMeasurements:

alAlkalinity. hardness, total residual chlorine. and conductivity weremeasured at the laboratory in each 100% sample. Daily temperatures weremeasured in one reolicate for each test concentration. Pre- and post-exposure test solutions were analyzed daily for DH and dissolved oxygen.

10. Statistics: Statistics were performed according to methods prescribed by EPAusing ToxCalc version 5.0 statistical software (Tidepool ScientificSoftware. McKinnevville. CA).

4

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

1. Results of a Pimephales promelas Chronic/ 7-day Toxicity Test.(Genus species) (Type / Duration)

Conducted July 7-14. 2004 using effluent from Outfall 101.

Test Percent SurvivingSolutions (time interval used - days)

(% Effluent) 1 2 3 4 5 6 7Control 100 100 100 100 100 100 10010.98% 100 100 100 100 100 100 10022.0% 100 100 100 100 100 98 9843.9% 100 100 100 98 98 98 9872.0% 100 100 100 100 100 100 100

100.0% 100 100 100 100 100 100Intake 100 100 1100 00 100 100 100

Test Solutions Mean Dry Weight (mg)(% Effluent) pcate number)(%Efun)12 3 4 Mean

Control 0.705 0.806 0.641 0.660 0.70310.98% 0.672 0.677 0.717 0.746 0.70322.0% 0.642 0.654 0.844 0.682 0.70643.9% 0.779 0.762 0.762 0.746 0.76272.0% 0.795 0.848 0.780 0.779 0.801100.0% 0.679 0.760 0.695 0.752 0.722Intake 0.847 0.735 0.794 0.689 0.766

IC25 Value: > 100% Calculated TU Estimates: < 1.0 TUc*Permit Limit: 43.9%

Permit Limit: 2.3 TUc95% Confidence Limits:

Upper Limit: NALower Limit: NA

*TUa = 100/LCso: TUc = 100/ IC25

5


2. Results of a Ceriodaphnia dubia Chronic/ 7Zda Toxicity Test.(Genus species) (Type / Duration)

Conducted July 7-14. 2004 using effluent from Outfall 101.

Percent SurvivingTest (time interval used - days)

Solutions 2 3 4 5 67(/oEffluent) 1 2 3 4 5 6 7

Control 100 100 100 100 100 100 10010.98% 100 100 100 100 100 100 100

22.0% 100 100 100 100 100 100 100

43.9% 100 100 100 100 100 100 10072.0% 100 100 100 100 100 100 100

100.0% 100 100 100 100 100 100 100

Test Solutions Reproduction (#young/female/7 days)(% Effluent) Data (realite number)

1. 2 3 4 5 6 7 8 9 | 10 Mean

Control 35 35 35 33 32 35 29 36 33 34 33.710.98% 30 35 35 34 30 34 36 30 31 34 32.9

22.0% 32 39 30 36 32 38 32 38 35 35 34.7

43.9% 32 35 34 39 41 36 39 32 34 35 35.772.0% 33 35 39 36 33 35 40 35 34 32 35.2

100.0% 41 40 43 33 41 36 35 40 36 41 38.6

IC25 Value: > 100% Calculated TU Estimates: < 1.0 TUc*Permit Limit: 43.9%

Permit Limit: 2.3 TLJc95% Confidence Limits:


*TUa = 100/LCso: TUc = 100/ IC25

6


2. Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test.(Genus species) (Type / Duration)

Conducted July 7-14, 2004 using water from Intake

Percent SurvivingTest (time interval used -__as_

Solutions 1(%MoEffluent) 1 2 |3 |4 |5 | 6|7

Control 100 100 100 100 100 100 100

Intake 100 100 I 100 I 100 I 100 100 I1

Test Solutions Reproduction (#young/female/7 days)Efflu Data (replicate number)

(%______ent) 1 2 3 4 5 6 7 8 9 10 Mean

Control 36 35 36 30 34 34 29 35 34 34 33.7

Intake 39 44 33 40 37 33 42 39 36 41 38.4

IC2 Value: > 100% Calculated TU Estimates: <1.0 TUc*Permit Limit: N/A

Permit Limit: N/A95% Confidence Limits:


*TUa = IOO/LCso: TUc = 100/ IC25

7

TOXICITY TEST RESULTS. UV-TREATED (see Appendix C for Bench Sheets)

3. Results of a Pimephales promelas Chronic/ 7-day Toxicity Test.(Genus species) (Type / Duration)

Conducted July 7-14. 2004 using effluent from UV Treated Outfall 101.

Test Percent SurvivingSolutions (time interval u - days

(% Effluent) 1 2 3 4 - 6 _ 7

Control 100 100 100 100 98 98 98

10.98% 100 100 100 100 100 100 100

22.0% 100 100 100 100 100 100 100

43.9% 100 100 100 100 100 100 10072.0% 100 100 100 100 98 98 98

100.0% 100 100 100 100 100 100 100

Intake 100 100 100 100 95 95 95

Test Solutions Mean Dry Weight (mg)(% Efsluent) (replicate number) Mean(%Efun) 1 2 3 4 Mean____

Control 0.771 0.628 0.607 0.775 0.69510.98% 0.644 0.701 0.695 0.606 0.66222.0% 0.739 0.728 0.640 0.722 0.70743.9% 0.784 0.667 0.661 0.685 0.69972.0% 0.641 0.642 0.702 0.682 0.667100.0% 0.791 0.796 0.728 0.706 0.755Intake 0.687 0.724 0.556 0.656 0.656

IC25 Value: > 100% Calculated TU Estimates: <1.0 TUc*

95% Confidence Limits:Upper Limit: NALower Limit: NA

*TUa = l00/LCso: TUc = 100/ IC25

REFERENCE TOXICANT TEST RESULTS (see Appendix A and D)Species Date Time I Duration I Toxicant R Results (QC25)Pimephalespromelas | July 7-14,2004 1500 7-days KCI | 536.9 mg/LCeriodaphnia dubia July 7-14, 2004 1125 | 7-ays I NaCl | 1055.1 mg/L

8

PHYSICALCHEMICALESMfMYWater Chemistry Mean Values and Ranges forPimephalesppmmelas and Cerlodphnfia dubia Tests, Sequoya NuclearPlant Effluent (SQN), Outfal 101, July 7-14,2004.

Test Sample D Tempetr CC) | Dissolved O.ygen(mgL) pH |Conductance Alkalinity Hardness Total Residual_ Initial Final Initial Final Initial Final (junhoslcm) (mg/L CaCO3 ) (ng/L CaC%3) Chlorine (mgIL)

Control 25.1 24A4 7.9 7.5 7.99 7.81 285 61 84Coto 24.4 -25.4 24.1 - 24.9 7.7 - 82 7.1 -7.9 7.41 - 823 7.33 . 8.06 258 - 302 61 - 61 84 - U10.98% 24.1 24.5 7.8 7.4 7.99 7.79 276 -

24.5 - 25.4 24.1 - 24.9 7.6 . 8.1 6.8 - 7.9 7.58 - 8.23 7.32 - 8.03 261 - 288 - . . .2 25.1 24.4 7.8 7.3 7.97 7.79 263 - -22% 24.5 - 25.3 24.1 - 24.7 7.6 - 8.1 6.7 - 7.8 7.58 - 822 729 - 8.06 246 - 280 - . . .

4 25.1 24.4 7.9 7.3 7.95 7.81 248 _24.4 - 25.3 24.2 - 24.7 7.6 . 8.2 6.8 - 7.7 7.55 8.19 7.27 - 8.08 238 - 261 - . .

7 25.1 24.3 7.9 7.3 7.94 7.83 22424.4 - 25.4 24.0 - 24.8 7.6 - 8.2 6.8 - 7.8 7.54 - 8.20 7.32 - 8.12 214 - 236 .

1% 25.1 24.4 7.9 7.4 7.91 7.91 202 78 89 <0.1024.3 - 25.5 24.0 - 24.8 7.6 - 8.2 7.0 - 7.8 7.49 - 8.18 7.33 . 8.16 195 - 214 77 - 79 88 - 90 <0.10 <0.10

Intake 25.1 24.5 8.1 7.4 8.13 7.89 200 80 91 <0.1024.7 - 25.3 24.1 - 24.9 7.7 - 8.7 7.0 - 7.9 7.55 - 8.54 7.33 - 8.17 194 - 215 79 - 81 90 - 92 <0.10 .<0.10Control 25.0 24.8 7.9 7.8 7.99 8.02 285 61 84

24.5 - 25.4 24.6 - 252 7.7 .8.2 7.6 -8.1 7.41 - 823 7.41 - 824 258 - 302 61 - 61 U4 -8410.98% 24.9 24.8 7.8 7.8 7.99 8.04 276 -10.98% 24.4 - 25.2 24.6 - 25.1 7.6 - 8.1 7.5 - 8.1 7.58 - 8.23 7.46 - 8.24 261 - 288 - . . .

2 24.9 24.8 7.8 7.8 7.97 8.05 263 -22% 24.5 - 25.2 24.5 - 253 7.6 - 8.1 7.5 - 8.1 7.58 - 822 7.47 - 8.27 246 - 280 -

24.9 24.8 7.9 7.8 7.95 8.06 248 -43.9% 24.4 - 253 24.5 - 25.2 7.6 - 8.2 7.5 - 8.1 7.55 - 8.19 7.47 . 828 238 - 261 . . .72% 25.0 24.8 7.9 7.8 7.94 8.10 224 -

_ 24.6 - 25.3 24.5 - 25.3 7.6 - 8.2 7.5 - 8.1 7.54 - 8.20 7.47 - 8.32 214 - 236 - . . . .100% 24.0 24.8 7.9 7.8 7.91 8.13 202 78 89 <0.1024A - 25.4 24.6 - 25.3 7.6 - 8.2 7.5 - 8.1 7.49 - 8.18 7.51 - 8.34 195 - 214 77 - 79 go - 92 <0.10 - <0.10

Intake 25.0 24.8 8.1 7.8 8.13 8.11 200 80 91 <0.1024.6 - 253 24.6 - 25.3 7.7 - 8.7 7.5 - 8.1 7.55 - 8.54 7.49 . 8.33 194 - 215 79 - 81 90 - 92 <0.10 - <0.10

Overall temperature CC)PimephalespwomelasCerlodaphnia dubia

Average24.824.9

Minimum24.024.4

Maximum25.525.4

9

PHYSICAL/CHEMICAL SUlMMARYWater Chemistry Mean Values and Ranges for the Pimephales promelas Test, Sequoyah Nuclear Plant Effluent (SQN), UV Treated

Outfall 101, July 7 - 14, 2004.

Test Sample ID Temperature (OC) Dissolved Oxygen (mg/L) (HS.U.) ConductanceInitial Final Initial Final Initial Final (Rmhos/cm)

Control 25.1 24.5 7.9 7.4 7.96 7.75 27924.5 - 25.4 24.2 - 25.1 7.6 - 8A 6.8 - 8.0 7.44 - 8.24 7.28 - 8.02 265 - 299

10.98% 25.1 24.5 7.9 7.4 7.93 7.77 27724.6 - 25.4 24.2 - 25.1 7.6 - 8.4 6.9 - 8.0 7.45 - 8.24 7.30 - 8.05 260 - 291

25.1 24.5 7.9 7.3 7.93 7.81 26622a 24.6 - 25.4 24.1 - 25.1 7.6 - 8.4 6.7 - 8.0 7.44 - 8.24 7.32 - 8.06 243 - 283

25.1 24.5 7.9 7.3 7.93 7.80 24743.9% 24.5 - 25.3 24.0 - 25.2 7.7 - 8.4 6.7 - 8.0 7.44 - 8.27 7.33 - 8.07 217 - 265

72% 25.1 24.5 7.9 7.3 7.92 7.88 225.__ 24.4 - 25.4 24.1 - 25.1 7.7 - 8.4 6.8 - 8.0 7.44 - 8.25 7.34 - 8.14 207 - 239

100% 25.1 24.5 7.9 7.4 7.92 7.87 20424.4 - 25.5 24.2 - 25.0 7.7 - 8.4 7.0 - 8.1 7.44 - 8.25 7.34 - 8.13 194 - 216n 25.1 24.5 8.0 7.3 8.01 7.87 201

Intake 24.8 - 25.3 24.1 - 25.1 7.7 - 8.4 6.6 - 8.1 7.47 - 8.35 7.36 - 8.13 192 - 211

Overall temperature (CC)

Pimephales promelas

Average

24.8

Minimum

24.0

Maximum

25.5

10

SUMMARY I CONCLUSIONS

Outfall 101 samples collected July 5-10, 2004, showed no toxic effects to fatheadminnows or daphnids. The resulting IC25 values, for both species, were > 100 percent.Exposure of fathead minnows and daphnids to intake samples resulted in no significantdifferences from controls during this study period.

Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fishpathogens present in intake water have been the suspected cause of interference (anomalous doseresponse and high variability among replicates) in previous toxicity testing at Sequoyah. At thetime this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples, suggesting that pathogenic bacteria were not present.

11

Appendix A

ADDITIONAL TOXICITY TEST INFORMATION

SUMMARY OF METHODS

1. Pimephales promelas

Tests were conducted according to EPA-821-R-02-013 (October 2002) using fourreplicates, each containing ten test organisms, per treatment. Test vessels consisted of 400-mL polypropylene beakers, each containing 250-mL of test solution.

2. Ceriodaphnia dubia

Tests were conducted according to EPA-821-R-02-013 (October 2002) using ten replicates,each containing one test organism, per treatment. Test vessels consisted of 30-mLpolypropylene cups, each containing 15-mL of test solution.

DEVIATIONS / MODIFICATIONS TO TEST PROTOCOL

1. Pimevhales promelas

None


None

DEVIATIONS / MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TESTORGANISMS

1. Pimephales promelas

None


None

12

PHYSICAL AND CHEMICAL METHODS

1. Regents, Titrants, Buffers, etc.: All chemicals were certified products used before expirationdates (where applicable).

2. Instruments: All identification, service, and calibration information pertaining tolaboratory instruments is recorded in calibration and maintenance logbooks.

3. Temperature was measured using EPA Method 170.1.

4. Dissolved oxygen was measured using EPA Method 360.1.

5. The pH was measured EPA Method 150.1.

6. Conductance was measured EPA Method 120.1.

7. Alkalinity was measured using EPA Method 310.1.

8. Total Hardness was measured EPA Method 130.2.

9. Total residual chlorine was measured using EPA Method 330.5.

OUALITY ASSURANCE

Toxicity Test Methods: All phases of the study including, but not limited to, samplecollection, handling and storage, glassware preparation, test organismculturing/acquisition and acclimation, test organism handling during test, andmaintaining appropriate test conditions were conducted according to the protocol asdescribed in this report and EPA-821-R-02-013. Any known deviations were notedduring the study and are reported herein.

REFERENCE TOXICANT TESTS (See Appendix D for control chart information)

1. Test Type: 7-day chronic tests with results expressed as IC25 values in mg/L KCI or NaCl.

2. Standard Toxicant: Potassium Chloride (KCl crystalline) for Pimephaks promelas.Sodium Chloride (NaCi crystalline) for Ceriodaphnia dubia.

3. Dilution Water Used: Moderately hard synthetic water.

4. Statistics: ToxCalc software Version 5.0 was used for statistical analyses.

13

REFERENCES

1. NPDES Permit No. TN0026450.

2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents andReceiving Waters to Freshwater Organisms, EPA-821-R-02-013 (October 2002).

3. Methods for Chemical Analnsis of Water and Wastes. EPA/600/4-79/020 (March 1983).

14

Sequoyah Nuclear Plant BiomonitoringJuly 7 - 14, 2004

Appendix B

Diffuser Discharge Concentrations of Total Residual Chlorine,

Diffuser Discharge Concentrations of Chemicals Used to Control Growthof Microbiologically Induced Bacteria and Asiatic Clams,

During Toxicity Test Sampling,

Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations ofChemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During

Toxicity Test Sampling,March 12, 1998-July 9, 2004

2

Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrationsof Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks,

During Toxicity Test Sampling,March 12, 1998-July 9, 2004

Towerbrom CL-222 PCL-401 CIA363; Cuprostat- H 130MmgAL 'w~~L$ mg/L 91gL, PF mlTRC X Copolymer mgF t

Azole< 0.002 o9 026" 0.009 - -

0.011 006 0.028 ~0.028 O42 ~ .009 .O6 -

0.008 4O)2> 0.009-0.006 g 0671 0.009 0.0, 0.109

< 0.002 <~2 ~ 0.009 -

< 0.002 :02~~ 0.009 -

< 0.00570.0190.0088

< 0.0088< 0.0076< 0.0152< 0.0141

.055

�� O055�

0.055�

0.05•

0.0190.0190.0180.0190.0190.0190.019

0004~""j"0.108

0.0143 '0050.020 05-~ 0.0092 'O02~ 0.020 0.'005 -."

< 00120 <Q.025.<k 0.020 -

iK<i < 0.0087 :.2 0.020 -

0.0120 : .05 0.020 .0-< 00036 '025 0.020 -

< <00036 025 0.020-

08/27/200108/28/2001029/200108/30/200108/31/2001

<>'<s 1<

0.017<0.0096<0.0085<0.0094<0.0123<0.005

O.O6�

V O�06�0.0s�

0.0210.0210.0210.0200.0210.020

!`0b.006`' o 0: 1

0021

1 1/2 5/2 0 0 1 ~< 0 0 04 4 ,-,

11/26/2001 -<001 19 >'A040.02 00-11/27/2001 0.0137 ~'~.930.019 O0711/28/2001 , <D00089 j 0 20.019 T.0

11/29/2001 0.0132 0Q40.020. 7-11/30/2001 < 00043 >0040.02 - L12/09/2001 ~ t' <0.0042 A -M

12/O12001 <001042 ~12/10/2001 ~ ' <00104212/12/2001 0.0128 0.024 .2 ~ .012/13/2001 <0.0088 ~ 9040.02 -

12 1 / 0 1 ~0.0134 > b 0 40.02 . 7-

3

Table B-I (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrationsof Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks,


PCL-401mg/L

Copolymer

0.020.0140.014

0.014

0.0230.0230.0230.008

4

Table B-I (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrationsof Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks,


Date LSum Towerbrom XPCL-!222>i PCL-401 VL33Cuprostat- I1439MInyoh~rt g/L txtft 3 mg/L ¼fgL PF mgL

mgU RC Phpbt Copolymer DA.MA/ tut'

06/15/2003 , < 0.0045 ~06/16/2003 <0.037 0057 0.020 - Kt0 306/17/2003 <008 > Ai1 0.014--

0618203c 0.0048 t0.40.014 - %9406/19/2003 < <00085 > O5S0.020 -t- 02506/20/2003 < <0.0048 <IOst 0.020 ___________

08/03/2003 It <0 0050 t~~-08/04/2003 ~ ~ <0.0050 j~00.020-08/05/2003 <0.0051 §< 057>Y\r 0.020 - 0208/06/2003 <0.0084 ~ ~5~~ 0.020 TY - -Th2

08/07/2003 ~ -0.0129 fOt 0.020 ~ .208/08/003 0.0153 K .S- 0.020 00

10/05/2003 j<0.0043 015> 0.020 r- -

10/06/2003 <0.0043 "O {057,<2 0.020-10/07/2003 - 0090 ctS; 0.020 - 00510/08/2003 .<0.0106 .073 0.020- 0.210/09/2003 ~ 0.0181 Kv.2t 0.022- 1210/10/2003 0.0183 O02< 0.024 00

02/01/2004 0.0093 <,.070.009 -

00124/2004 .0200340.009 0090203204<0.0034 K 96 0.009 '-t -

02/06/2004 0.010524)2 0.009 00105/05/2004 <00014492 0.009 -- 42

02062026.0050.014090905/06/2004 <0.0146 4026O37' 0.0139 4205/07/2004 <0.02210454 0.0201 0094 .205/08/2004 0D0146 G00 0.0213 i-05/09/2004 <'<0.010227 '.0S 0.020-

07/04/2004 0.02176 oos 0.021907/05/2004 M<0.010854 O 0.020 00

07/06/2004 <D000785 O~ 0.020 - 03

07/07/2004 ~ ~ 0.0252 7056V 0.019 - <0307/08/2004 ;37 0.0223 7: 7 0.0 19 0007/09/2004 70.0182 0.020 00

5

Sequoyah Nuclear Plant BiomonitmingJuly 7 - 14, 2004

Appendix C

Chain of Custody Records andToxicity Test Bench Sheets

BIOMONITORING CHAIN OF CUSTODY RECORD Page _1 of 3_

Client TVA Environmental Testing Solution, Inc. Deliveed By (Circle One):

Project Name: Seqoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client

P.O. Nmnber N/A Asheville, NC ot (specify): Expre comier

Facility Sample Sequoyah NP 28801 General Comments:

NPDISNumber TN0026450 Phone: 828-350-9364 At &0 <CollectedB yWanda Alen Fax 828-350-9368

Field Identification I C (ib/Camp. e): D R cine Flow goo.Sample Description Niumber & (MOD Reain Event? .. . -

volume )(Mark as Appropriate) ...

Collece w.d .7/0..t"1.DeTWOe Yes NfYe. No Truce ~ 4 .e~

SQN-0-TOX Comp 7/5/04 - 7/6/04 92(2.5pl) MA

SQN.INT-TOX Comp 7/5/04 -7/6/04 09qA/0V 1..5 1.N

sample Ctoy-FMf In From Top Down ____________

Relinquishted By (Signature): Daterhme Received By (Signature): Date/rimne

Wanda Allen 7/6/2004 7/6/2 004 _

Express Couder 7/6/2004 7/6/2004

._ l "tBructions- Clients should fill n all area except those in the 11aboaty Use" block Biomo ng samples are preservd by stoing them at 6C and spping than in ice. The hold time for ech

sample is 36 hours fiom the time of. cllection Theadore, please collect and ship in aud a way that the laboratoxy will receive the samples wiYt ample time to initiate testing witbin that time fimeSamples shipped ovught an Friday via FedEx or UPS most be mared for Saturday delivety or ty will not arrve until the following Monday.

Environmental Testing Solutions, TIM

panIWhole Effluent Toxicity Sample Receipt Log

Date *Um Red Retieyd e SOWle Projet Sample Smple none Ead desripteio Comests

Ide'eted eeds by f tep c (C) e bee.

_-06-4 1356 1. Slmner _ 5.0 120 040706.01 Euta DtvisNC 8Sapl Red w 4-h of colon

07-0604 1338 J. Sori 1. 1321 040706.l TVA- Sqdeh NuclearPlnt. Oual 101 N

01.06-04 1338 1. S3ayer Comw L6 1321 040706.03 TVA - S oh N arPlant- Witk TN

07-06-04 1630 J. S.umnr D. Ellkd IA 1.322 040706.04 _ro Caroknss - _heb4ib Ash Pend NC

0740 GE

BIOMONITORING CHAIN OF CUSTODY RECORD Page _2_ of _3_

Clien TVA Environmental Testing Solution, Inc. Dmroed By (Cirrle One):

Proect Name: Sequoyah NP Todcity 351 Depot Street. Fod1tx UPS Bw Client

P.O. Namben. N/A Asheville, NC oti (sp*): Express Corier

Facility Sample& Soquyah NP 28801 General Comme:Cu.40e(i5.kd.S-g~LiA~ t

NPDES Nwmb TN0026450 Phone: 828-350-9364 f

Collected By Wanda Allen Fax: 828-350-9368

Field Identification Grab/Cmp. Conllction Daterime A tner FlowSamiple Description, Number & (MOD) R~nEvmVt

.Mr K as Appropriate) A Z .

- -Ti me . ........ es Y% NO To =

SQN-101-TOX Cbmp 7n104- 7M04 o qh3 2(2.5gOl NA_

SQN-lNT-TOX Comp 7/04 - 7/8/04 51,5 gl.) NA

Sample Cu - Fil In From Top Down

Relinquished By (Signature): Dateim Received By (Signature): DateTime

Wanda Allen 72004 Expss Cu 7M22004

Express Counier 7/2004 E' m ETSu 5, 7M200 1007 .

Instuctions: Clients should fin in all ams except those in the 'Laboratory Use block Biumonitoring amples we preserved by storing tham at 60C and shipping them in ice, The hold time for eahsample is 36 hoirs ftom the time of collection Therefore, please collect and ship in muh a way tht the laboraoty will receive the samples with ample time to i tie testing withinthat time fimeSamnples shipped overnight on Friday via FedEx or UPS must be marted for Saturday delivery or they will not arrive until the following Monday.

EmlromnentlTeatng Solutions, Inm.

Path

Whole Effluent Toxcity Sample Receipt Log

lnedv aw *7Ir maOIr10 I0"J s n 14 3mh rjmtSape apha"dudp IN"074044 0941 - J. - S a- El 132 04070 101 _ _- _M "IN

4 i 2300 *'evOum b -_ 07"4 US 10 K K 040702.03-WVA-:5yubNUckuPkli .o b

07e 4

BIOMONITORING CHAIN OF CUSTODY RECORD Page _3_ of 3_

Client: TVA Environmental Testing Solution, Inc. Die By (CrcD One):

Prect Name: Sequoyah NP Todcity 351 Depot Street FedEx UPS Bus Client

P.O. Numbei N/A Ashville, NC o ( Express Courier

Facility Sampled: Sequoyah NP 28801 General Coments:

NPDES Number. T40026450 Phone: 828-350-9364 4 omr SeQ Recerez

CollectedByrWandaAllen Fax: 828-350-9368

Field Identification I Orab~Cmp. iCollection E~eErnt? 94PW'nf .n.wNumbes cMidtion ON (MOD) Rain Even

. .. .... ....

SQN-101-TOX Comp 7/9/04- 7110/04 2(2.5g) NASga ) NA

SQN-MT-TOX 7/9o4 -7/10/04 YD.... ... '. _

_ Saple Custody - Fill In From Top Down _

Relinquished By (Signatu): DateTme Receiv By (Signatur): Datefllie

Wanda Allen 7/10/2004 Express Courier 7/10/2004

Express u [70 H'1 711t2Express Counier. 7/=0 12az Erm r-4 L"P7nno4 7110/2004 132

.

...

lnsuctions: Clients should fill m all areas ecept thse in the "Laboratory Use" block Biomonit g saTples are preserved by torg them at 69C and shipping tm ioe. The hold time for eachsamnple is 36 hours frm the time of collection. Theoroe, please collect and ship in such a way that the laboratoiy will receive the smles with ample tine to iniiate testng within that time flume.Sapes shipped overnight on Friday via FedEx or UPS must be markd for Saturday delivey or they will not arrive util the following Monday.

Envirenneidi Testin Sdu6lem I'm

Whole Effluent Toxidty Sample Recelpt Log

Dm _h l Ro Ivd Reird . mpG. P;;3 m.... Smpk m d dinuCpd..

07-1004 0942 0K73 H_1_OI M Ei1__. StMRPNC0-100 -6-2 2.3 1 04a7 10.02 C -_. Ste_ S C

07.10o04 0942 .r Ke Fed- E 3.7 1325 04071M.01 Hawhy ePadMcCmo*etwuehyitd= N _____C

07.10.04 0942 K 1 Cm M. Ex 3.6 1326 04010Q04 Duhe w Cmcrslon - Fb4 ud SftmSIo lie

01-10.04 0953 UPS Q7 1329 0M47EL.03 T2M be. - P C_ W8A NC

07-10404 e953 05S Q4 1040710.06 mid 1 le - ML 0o p l_ Com

07-1004 1232 L _nm 2.4 1321 04Q7100 TVAVA- - Nb Sa. Om lol 01

.10 C4 1232 1. L W e i 3.1 1311 047 1f011 TVA -Se Nuce SW=. Nugk ST oN

07.104W

. +

Environmental Testing Solutions. Inc. Page I of 6

Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0)Species: Pimephalespromelas

Client: IVAFaciity: Seauovah Nuclear Plant - Non-treatedNPDES #: TN 0026450Project#: 13231

Dilution vreparat In ffo rmation: |Comments:Dihition pV (%) . 10.98 22 43.9 72 100Effluent volum (niL) 274.5 550 10975 1800 2500 -

Diluent volume (nfL) 2225.5 1950 1402.5 700 0Total volume (niL) 2500 2500 2500 2500 2500

Test organsm Information: Test Information:Orgnism aMe: I 25.5 ro 2-5. - A4S OA b Randomizing tenylate: uelln4Date and times organisms 01 0.OL4 J3 lb I6 EST Incubator number:were born between: oOrganism source: 01. nO-Otk AS% 6fTCA Alremla lot numbern erIO6sSTransfer bowl information: pH - Temperature - C Total drying time: Z4-cs

I.-S_ 2'.S Date/ Time in: C-a-13-c 1L4&0Average tinsfer volum: Date / Time out 61" rA!5o_ Oven temperature: W0' C

Daflyfeeding and renewal Information:

Day Date Mornalng Afternoon Test Initiation, Control water Sample numbers Analystfeeding feeding time renewal, or batch used used

time termination time0 pD-0 4 a 43sO0OzI-O

___DQ S &SS sao J40Q Oi-ow-0o' Dq~oiczFi .0o A(2 O 2S rsb Y4MO 01- ot3O"OO.M 1.05 AZ

a Ol S .25&n. I 0S C-OW-04 3NMOe.OzI. 03

494 t-- >t SCo 1346 0:V 4Nio,V 0>w~ta.r .e

SP INL Ob i;S2 NCO 0106tA 6YO-M.01 /.00oe KEY6 Isis 9 lS2S I4S O-ID.o4 0- I.06 V

7 X-k X,,,,,.;;K,, aLXq ORX

Control information: AI ptancc c Summary of t en dpolnts:%Mortlitr. I _ 7 SW% 7-day l>o 1ŽAverage weight per initial larvae: 6.105 1. f NOEC ItblAverage weight per surviving larvae: O.,101 2 0.25 m5e LOEC >

CbV >h MMcfIC2 j >: I El

Environmental Testing Solutions. Inc. Page 2 of 6

Species: Pimephalespromelas

Client: TVA I Sequoyah Nuclear Plant - Non-treated

Date: O? t) O

Survival and Growth DataDay CONTROL 10.98% 22% -

A B C D E F G H I | K L

1 o ,1 10 10 101to 1to I ,o t o to 11O 10 10 101 to 10t to 10 10 (ID

.210 IOg Io ) 10 j0 1 O 10 IC 10 /O

34_ 1_ to to /( 0 /O /0 10 1O /0 /O

_____ /O to O 1/ to . /O t o to to /O 10

cto 1/ /O/ 10 /81_16 /8/c / 0/O

6o JO /O /O /0 'o 'o 10 q /O /C

7-~sd~( -… ___ _ 1010 t0IC) 10 °)/o, q ,,0 ,A - Pan weIght (mg)

B - Pan + Larva weight

__ _ __ _ _ _ _. _ _' _ _ _, _~" ' i~Lasr v a cw ve lgb (mg) - A - 2 I eWegt per Initila nmber of 6 4A 0* 0

mCIInItlaI umbereflarvae O O'U _ 0 0* -0Average weight per Initialaumber flame (mg) O.a705 0:105 O.70&

Calculations and data reviewed:

Comments:

Environmental Testing Solutions, Inc. Page 3 of 6Environmental Testing Solutions, Inc. Page 3 of 6


Client: TVA J Sequoyah Nuclear Plant - Non-treated Date: f70t2

Survival and Growth DataDay - 43.9% - 72% - 100% -

M N | OT |.R S T U V W X

/0IO 10 it) /to 10 10 /o t /0 ,o1 10. 10 lO J0 10 lo l0o 0 ID 10 10 /02

t1 tO /1t (c) /a /0 O 1_

3_ 0 to /O -D /01t o0/0 _0 _0/0 _I

S_ / 91 10 /' 1 10 /0 /0 /0lO IL

___ 61 LA/0 I 10 10 1o 10 10 (0 - o _

7 /0I I> to lO 1o 10 _ 10 10 _ _

o 1- la ig ( ) _ IL) too -- 1 _ _ I_A - Pan weight (_m

3mIazi+ Lmarveweight

Larvaeweight (mg) -A- B ~ ~ , ~ ~

Weight per Iaittal aszmber of , ,~L L ,**e f, Sj.arwae ((g %UlV-ClIntlala*mbereflarvae 0' 0O O' _ O 0 'Aveage weight per hItilanniberdsbre(mg) OW1L 0 t

Calculations and data reviewed:I .III .

I Z, .- .. . .

Comments:

II -

ElEnvironmental Testing Solutintn TInr Page 4 of 6

Species: Pimephalespromegas

Client: TVA / Sequoyah Nuclear Plant - Non-treated Date: M -nIn 4

Survival and Growth DataDay 150% Intake

_ Z AA BB

0 . .1 /0 /O

1 /0 /O lo lO

2 /I /0 16 /0

3 /O to 1O 1t4 _1010 /0 (O

.S / 10 16 16

6 to to 10 /0

A - Pan weight (mg)

_ _ _ _ _ _ _ _ _ _ _ _ o 1b

B - Fan + Larvae weight

(mg) O tx O,-* Z

Larvae weight (mg) - AD- B ox I th

Weight per Initial number of 4 A $larvae (ng) 4A-4- C 1 lnitial number ot larvae OO 0 O 0Average weight per initialnumber or larva (me) O.-o)

Calculations and datarviewed

Comments:

I

TVA / Sequoyah Nuclear Plant - Outfall 101Non-treated

Chirnfe Whole Effamat Teft Test WEA41-R.02-013, Metid 1090.0)Spedys: PImhahspremday

Quslity CentralVerfftion of Data Entry, Calvaisffens, and StntWIraAnslym

TVAISsq.syS� Bud's? P1ud-Wu's�ut.(

Twtis-u 1*144,2604

_rqw : mm 1321 ltw~br4wqmtEe��- - - -

-gh - . _- ht _- -t4_

A 10 10 14.95 22.00 7.05 0.705 0.705Co B 10 10 15.00 23.06 3.06 0.06 103t.5 0.06° 166. 9763 tQS l6yC 10 10 15.10 21.51 6.41 0.641 0.703 0 0.641 ie* 073I eap~o

D 10 10 15.30 1.90 6.60 Q660B 10 1O 14.3t 21.9 6.72 0.672 0672:

I9,9F 110 0 14.53 21.30 6.77 0.677 0.703 5.0 0.677 1IIQ 10" .o 10 10 1 14.53 21.75 7.17 0.717 0.717H 10 10 IS.09 22SS 7.46 0.746 0.746I 10 9 14J86 M1.2 6.42 0.713 0.642_

K 1 10 14.39 23.33 3.44 OU4 0723 11.6 0.63544 976 3.10 14.S3 6.t2 0.62 0.632

M 10 10 14.U4 22.63 7 .7 0.77 Q779-o&% I 10 I t 14.51 22l15 7.62 1 0.476 0.733 5.7 0.762 7 &6 i

lo 1 0 1 0S31 2213 7.62 O o 7 0.762 7. 9.762 LS 44_ P 10 10 _15.04 _22 7A46 Q146 0.746

10 10 1 4 S 2251 7.95 0.795 0.7957 R 10 10 14.76 253. BAB 4.1 OS4S.

8 10 10 14.76 22.56 7.80 0.730 410.340T 10 10 15.24 23.Q3 7.90.779 0.77~9

U 10 10 159 017 6.7S 0.619 _ 0.679- - -U-9- -1% V .10 10 143 224 .0 0.760 o5 .s 0.760 lots 6.722 5. 4.A

W 10 10 15.06 -226.9 0.695 0.695X10 14.932 0. 7.52752 - _5__

I _ __ _ _0.347Y 10 10 148 23.32 *.47 O.U47OU

to10 10 14 22.13 - 7.35 0.735 Q 766 9.0 0.735 69.6 9.74 99. -9.AA 10 10 1.74 22.44 7.94 0.694 0.19_PR2 20- 10 14.74 21.63 1 6.39 0.3 ______ 0.659

DMs 1D Pl

PM . -13.p

3.D- M S Dtp3.D Po fiSp D

maimmumoff '.bis . htbrm d b. bdclammumspipmk ff d Ownfosa ms h e 14.3%rn 9sst mOnmeoreIttoeIId_LmwPlMbvesdd~uaktbytslMA(10&pwsosstI) -94%UpWrIDbaddsi byUMPA(90tpw-rnI.)-35%

a IzeroieA g2 0 tu gd u h 9 iLe Ht

USEPA 2000. tkdudi W kgssd Aese 1i g V4eC V an Zfhb Ta T b icthD B IP B 43- 3. US _c 1a Chcad C

TVA / Sequoyah Nuclear Plant, Outfall 101Non-treated

Statistical Analyses

L1r1 Ish Gnrwth and 9urvIm Twt-7 Dy GrothStait Date: 7/7/ZoO4 Test 1D Pp.RCR Saile 1> TVA / Sequeyh Nuclear Mlat - Outfall 101End Date: 7/1004 Lab MD ETS-Envir. Testing SoL Smvle Type: DMR-Divchage Monitoring ReportSanVle Date: PtotoooL CHRONIC-EPA-821402.013 Test Species PP-Pirpbles promelasComnfn entm ON-TRRATED

Conc-% 1 2 3 4D-Control 0.7050 0.8060 0.6410 0.6600

10.98 0.6720 0.6770 0.7170 0.746022 0.6420 0.6540 0.8440 0.6820

43.9 0.7790 0.7620 0.7620 0.746072 0.7950 0.8480 0.7800 0.7790

100 0.6790 0.7600 0.6950 0.7520

-

Transtfm: UntraufornedcOne-% Mea" N-Mean Mean Mltn Max CV% ND-Control 0.7030 1.0000 0.7030 0.6410 0.8060 10.487 4

10.98 0.7030 1.O000 0.7030 0.6720 0.7460 4.983 422 0.7055 1.0036 0.7055 0.6420 0.8440 13.301 4

43.9 0.7623 1.0843 0.7623 0.7460 0.7790 1.768 472 0.8005 1.1387 0.8005 0.7790 0.8480 4.060 4

100 0.7215 1.0263 0.7215 0.6790 0.7600 5.613 4

1-Tit-stat CrH

0.000-0.064-1.515-2494.0.473

iBed 0oftlnteal MSD Mean N-Mean

0.7349 1.00002.410 0.0942 0.7349 1.00002.410 0.0942 0.7349 1.00002.410 0.0942 0.7349 1.00002.410 0.0942 0.7349 1.00002.410 0.0942 0.7215 0.9818

AMdMM Teats Statitm Critical skew Ku8tSbphFo-WiMk Tes indicates omuI distribution (p > 0.01) 0.90164709 0.884 1.27209847 1.88498869Batleftt Test indicates equal variance ( - 0.07) 10.0637131 15.0862722b dHypotheds Teat (1-tf, O.05) NOFXC LOEC ChV TU MM)Q mMbDannett's Test 100 >100 1 0.09423493 0.13404685 0.00647958 0.00305788 0.10993567 5,18Treatments vs D-Control

sqn_07-07-O4data

TVA / SCquoyah Nuclear Plant, Outfall 101Non-treated

Statisdcal AnalysesJbwJb*"riffftuI"Rumnkv)

_ , D AS% CaThO) sow

MU

1200ICUXMO05

>100>100>100>100>100>100>100

0 20 40 60 so 100 120Do" %

D..e-Reqm lt

no .I

0.7&7 T 1

.. .... . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . . . . .0

1-WL 0.05 WmOf fnfw"iI 1

0 2

0.1

a,I9 U

sqn_07-07.04data

TVA / Sequoyah Nuclear Plant, IntakeNon-treated


Larval Fish Growth and Suvil Test-7 D" GrowthStart Date: 7/7/2004 Test Mt PpFRCR sanmle MD TVA I Seqoah Nuclear Plant - IntakeEnd Date: 711U2004 Lab Mt SEnvr. Testing SoL Sanvle T1e: DMR-icharg. Monitoring RepotSanple Date: Prolcol: CHRONC-WA-821-R-022013 Test Spedes PP-Pinopbalos promelasCommnents NON-TRIATED

Csne-% *1 2 3 4D-Control 0.7050 0.8060 0.6410 0.6600

100 0.8470 0.7350 0.7940 0.6890

-

-

Transformu UntransformsedCORe-% Mean N-Mean Mean Mh Max CV% ND.Control 0.7030 1.0000 0.7030 0.6410 0.8060 10.487 4

100 0.7663 1.0900 0.7663 0.6890 0.8470 8.990 4

1-Tot-Stat CON

-1254

fled ISOtimhleat MSD Mean N-Mean

0.7346 1.00001.943 0.0980 0.7346 1.0000

AnuOHary Tests Statistie Crieal Skew Kuirt

ShapliO-Vrkb Test i"cates nonrml ditnbution (p > 0.01) 0.92910469 0.749 0.54944252 -1.1099798F-Test ediates eqnsl variances (p -0.91) 1.14550662 * 47.4672279Hypotheds Test (1-ftt 0.05) MSDO mgDP MSD MSE F-Prob dfHoemocedastc t Test inicates no gnificant differences 0.09803075 0.1394463 0.00800112 0.00509013 0.2565693 1,6Trembments vs D-Control

sqnO7-07-O4data

TVA / Sequoyah Nuclear Plant, IntakeNon-treated

Statistical AnalysesUmWktv,,, ms (l R-any)

SByvl.h % gm 9% CbSS a.,

mcsYIcOMCSIC15J,20

X50

>100>100>100>100>100>100>100

4 n.

- _

as0.80.7

a 0.4

CK0.2-

0.1-

0.041

.2 Z

I . . .

0 20 40 so s0 100 120Doe %

D-'R P e Im

no -

0.7

I 0.310.2

0.1

&I d

14. 0.05 lewoCfsikwm

01

I

sqn_7-07-04data

EnirnenalTstigSoltos Inc . Page I of 7Environmental Testl�g Solutions, Inc. Page lof 7

Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1002.0)Species: Ceriodaphnla dubla

Client: fVAFacility: SequoYah Nuclear Plant - Non-treatedNPDES #: TN 0026450Project#; 1521

Dilution Irearation fnormation: Comments:Dilutionpep(%) 10.98 _ 22 _ 43.9 _ 72 _ 100Effluent Wolu=e (mL) 274.5 550 1097.5 1300 2500

DiiUntvolume(mL) 2225. I 1950 1402.5 700 0

Total vohme (E)L) 250 2500 200| 2500 2500

Test organism Information: Test Informaton: |Organism age: & * £ s- nmizing template: G 6 1Date and times organisms on.Cn.ej csnL4O -M itl Incubator number andwere born between: shelf location: 2 BOrganism source: Mr-- t- CYCTbatch: 1e5 -cki-iTransfer bowl information: 2.pH -1.2 Temperature - V.'I 1 Selenstrum batchAAs W.z< tH

Daily renewal Information:

Day Date Test Initiation, Control water Sample numbers Analystrenewal, or batch used used

termination time20 n qp m.q _ lgC O4 VOi.OZ. | As

3 f. I O j (1 lCt.oq oD706.OZ;. 1.03 ,

4 ___1__ I OO 0 .o-OIWCf 01 iot .1 0T A3

* 5 O?.IZ-Dq 111 . 0 0-6-01 C 10 . I co.t8 1 _6 __1___ I0 m10.1.0.m J F7 c. A

L . .. _

Control Inform ation: Acceptimce crtefa Summar of stendpoints:% of Male Adults: T V. I CffZ s20A 7-dayI.C50 L>CS %% Adults having 3d Broods: /1 _ z 50% NOEC 1007.,

Mean Ofspring/Female: 2 .1 :11 a 15.0 offisrngea ChV > =%

Y. CV: _ _ _ 7.a74 <40.0% IC25 > I Z.

Environmental Testing Solutlons. Inc. Page 2 of 7

,i

II.

I1.

I1.

1 ,. .

I -

I"1:.Ii.i

Species: Ceriodaphnla dublaClient: Seauovah Nuclear Plant - Non-treated Date: 0J; -3-4 ...CONTROL Surnival and Reproducdon Data

_. Replicate numberDay 1.. 2 . .3 4 5 6 7 8 9 lo

I Young produced 0) Q .Adult mortality U- %- U- %- U U L W U

2 Young produced j O C Q l =) j Q 0

Adult mortality 1 L _ - L __r

3 Young produced [| C _ _ __ p no I c- totUt tlIy %-- U e | -

4 Young produced ' H S S Lt _ _ 4 4Adult mortaUty -- t. . L- C _. L-. L.

5 Youngproduced I ' -Z- I 10 I 3 | lZ LBAdult mortality t U U. I L. U.- L L- - L. L.-Youqg produced 5 6 O C) O 4_ I _ 0 I O ,Adult mortality _U * U- U. U. -

7 Youngproduced *_ 1 IT l i TIq i's5 L 1 In 1''Totalyoungproduced -SS T5 b a 7T 3S z9 |36 33 34Fnnal Adult Mortality L 7 * I > I -7 ? | t * u

X for 3' Broods t r X @ L § zC 1 > >

Concentraton: |% Mortality | O7, |MeOn O ngAFernale:m _ 1

CONC: 10.98% Survival and Reproducdon DataI Repllcate uumber

Day | I 2 3 4 5 6 7 8 9 1T1 Young produced | - -

Adult mortality 4U. .U2 Young produced

2 Adult mortaity L L I(

-m- I.- L L3 Young produced I ) 0 L 0_

Adultmortaliy - L L L LL- r - -- - -

41 Young produced LIC) . .,~ .( JIL L14 toAdult mortality ~ L JL L L- L- t-

s Young produced lo 9..7) A.L c..). -0 . 0

S - - -* - a - - -. -Adult mortality I L U L - L- U. -U U. U -U - t ?- U.7 Young produced ICAjj 1 1 ,J1j 8 FItF 1

Total young produced ;M laS I ssIso a31 3b1 q & 3o013\ I 'I t'- I C I C I 7. [ I C I =, 1!> 7 C 1 -L I C.

IConcentraon I%Mortality I I.

|Mean Offspring/Female: I 32A I% Reduction from Control: Z. V1. 1

Environmental Testinty Solutions. Inc. Page 3 of 7

Species: Ceriodaphnla dublaClient: Secuovah Nuclear-Plant - Non-treated Date: -CONs 22% Survival and Reproduction Data

Replicate number_ _ _ I 2 3 4 5 6 7 3 9 101 Young produced 0 £ ) f C) O OE3EElm __

Adult mortality L- t-~L...~ L- ongpodcd ) UYoungproduced f) l. ) c)Adult mortality L t- L L (

3 Youngproduced CI C I OOAdult mortality r L. I L.. .- .

4 Young produced S11 Is Is .. Y. 5 SAdult mortality 1- | W - L -X L

S Young produced I to to lS lO I E I ZI OAdult mortty I _W.

- .- - --- 1.. 6 Young produced . O I 0 Q

Adult mor~trlt k. | . %- L |

7 Young produced P K 11° 20) - IC I' It 11Total young produced 3 2 A 60 afo az 43 ;!?. 68 i S 5 5SFial Adult Mortailty 1 , 1 -t

i Concentration:%Mortality:

| Mean Offspring/Female: | 1.-1% Reduction from Control: - S.07.

CONC: 43.9% Survival and Reproduction DaIRepltcte number

Day 1 2 3 4 5 6 7 a 9 10I Young produced Z) -n a I7o

Adult mortality | - L|- L L- L. |

- -_ _ -_ _L -XL2 Youngproduced | f__-0 0- Adult mortality | L L L.. |- L | | L |

3 AdlYortlt J~ ung produced - - -

Adult mortality L. L || | LW | | |

| Youngproduced 10 j3 |J - - - .L 0___

Adult mortality L L- L L- L |- L L| L- LS Yonng produced |0 I3Z 16 85- I I ||

Adult mortallty L- | L- | | L L- U |. -| | |7 Young produced 1 ( J~i I,20 11

Total young produced aZ 3 a'! a, q 1~ 31 I, -9 2~. jq B53Final Adult Mortl ET I ~ I L.= L ~I L-

Cncentraton:% Mortali t:

|MeanOffsprnw/Femnale: | 35.11I % Reduction from Control: I -SA7I I

Environmental Testine Solutions, Inc. Page 4 of 7

Species: Cerlodaphnla dblaClient: Sequovah Nuclear Plant - Non-treated Date: Oif . L.. .CONC: 72% Survival and Reprodudion Data

_ |Replicate number_D2y __ 2 779 1

Youngproduced 0 O 0 O | 0 0 a

Adult mortality C. I-__- V.- -l

2 Young produced 0 0 ) C)Adult mortllty L (, 1 ( .L L L- .

3 Young t produced. -CL>. Adultmort-ty - - - L - _ - :E

4 Young produced 4 bQ |) S s S SlAdult mortaity LL. TJ IU t1 .I I

4 Youngproduced 4 _ _3 I I to 1$ lb Is1 10Adult mortality %.- U -L. * I L- L- L- . .- -

Adult -ortaty -- L--- - %-7 Young produced I 6 2 0 19 .I zO 1. i 18

Total young produced 25 5 wS 5 - 3 so b as 1 O S'S _Final Adult Mortality_ _ . U U

rConcenradon : .I % Mortality U U .|-M-an---rng eale: -S. 7

coNC: 100% ' Surrvival and I 0produd1tion DataI 11 Repllelte numberI

IDay 2 3 4 2 | 73 9 1

IoaYoung produced 7 a n s

|ial Adult Mo rtali ty L| L | |L

2 YConcgntration:

_IAdult ort lit|

| 3 Meu Yng producedO|f|O: O 2 l

AdutortatY L L LReuI L - l - L-I . 1

4OC Yo00% purvdvdead Repr-d'sion Dat

1 Young produced J)pq I' 1 ' I' CL- I.

Adult mortality L| L I L I L L I2 Young produced ~U

Adult mortality L-7

7 1Young produced - I -0I2 1JqIo 2 -

Toa young produced *~(

AlulAdultMortality |L| L L 1- | U. | * |-

wxUA .veC

i

I.I

Concentration:% Mortality: - oMean OffpnngFemale: Iat-'Y Reduction from Control: - 14.52

Environmental Testing Solutions, Inc. Page S of 7

Species: Ceriodaphnia dubiaClient: Senupoyh Nuclear Plant - Non-treated Date: ... .n 7-IDLCONTROL Survival and Reproducdon Data

lie Ilcate sobe

Day 1 2 3 4 5 6 7 a 9 10

1 Young produced _L C) O 0OAdult mortality .. L__1

2 Young produced Q V 0 0 I O O

Adult mortallty I J s L L C L3 Young produced _ ; C

Adult zortallty 1 L L . ' I - '

4 Young produced %W - S - - to Sc - .

Adult mortality I L. l -1U C.. -1 LS Young produced It I2 to 1VI ta

Adult mortality -L- L '

6 Young produced -Q -_Adult mortality - %-- C . L 1 L i | >

7 Young produced IrT r I S Zo - -

Totalyoung produced - A 5 _ 34 ZL( z _35 __ a

Final Adult Mortality _ t L , _ -I C. _

| Concentrad on:% Mortalii 0Mean Offspring/Female: 33.1% Reduction from Control: _P,

orNc: 100% Intake Survival and Reprodaction Data- - _ i- teumbe

|_Day II 2 3 4 5 6 7 * 9 10I Young produced c|.= = L. C|) £ ||C

| |Adult norhality L UL 4 - L- L-w1e1W2 Young produced = { mm.. - -C °°|Adult mortality L . j .. C.LIC

_3 _Yung preduceda 0 ) To O- Adultmortality I tL ,t L | L I L -L1 _

Young produced LILI -I-Adult mortality L

s Young produced | S | -t- 7oIAdult mortality L -_ | L C L-S Young produced C)I fl vs 0 .. -IAdult mortality t - % - %.- %... %-.. L t

6 Young produced _. . . . I 1 19 0 0 .. % . .I

I'CA'IMa<

Total young produced z°q I qq1 .5 q I 3 -1 I a3 b IN &LZI si I 5i. I q- I- - 1 eJ - -IL-I I C I C-1 L - 11 CI -J CI C

I Concentration: I% Mortaliw. 1 1.Mean Offsprin emale: I I% Reduction firm Control: I - I5.I;


Verification of Cerlodaphnla Reproduction Totals

Control-i

Dby 1 2 3 T4 1 S 7 8 1 9 1 TlO U O O O O O O O O O -1 0 0 0 0 0 0 0 0 0 0

2 0 0 00 @ 0 T i ~ T o3 0 0 0 00 0 0 0 0 0 04 T5 4 5 54 5 4 44 45 1 15 12 12 11 10 10 13 12 13 119

02 ±2 0 10 00 17_ _ .i 186 _1 19 1917 - 1 73

Tel.! 35 3535 33 32 3523633 34 337

10.98%R- *hme -- S -

Day 12 3 45l

lO " O O O U 0 1 a 1 ° 00 0 0 0 0 0 0 0 0 0 02 0 TT O TO T TT 6- 0 0

4 5 5- 5 6 5 5 4 5 5 3 5010 0 01 11 14 o 115. ~ s T T T so OO°7°1°°

sT0 0 oi 0 0-00 07 15 is 17 18 14 18 18 15 14 17 164

T33 3 35 35 34 30 3433| 31 34 329

22%

Day 2 3 4 6 7 TTW '11 '

1 0 0 0 0 0 0 0 0 0 10 02 0 O 0 00 0 0 0 0 0 03 0 0 0 00 0 0 0 0 0 04 5 5 4 5 5 6 5 5 5 6 51

12 13 10 11_L10 14 121 10 10 119- - 0 0 ± 0 ± 0 0 07 1s 19 16 20 17 113 18 i 20 19 177

TOWm 32 39 0 3 32 38 31 38 35 35 347

43.9% __ _ _ _

-a *S 6 7 8 9 S I o

1 0 0 0 0 0 0 0 0 0 0 02 0 0 0 00 0 -TTTT 03 000 00 0 * O4 56 4 6S- 5 5 5 4 6 515 10 13 14 14 16 12 IS 11 10 12 1276 0 0 0 0 0 0 0 -if-i--i 0

7 17 16 16 19 20 19 19 116 20 17 179Tel. 32 35 r34 39T 4-1 -34 3 ~32 34 35 357

72%

" 1 2 3 4 ±5 7 I a T10e !1 0 0 0 0 O OI ° I O

2 00 0 0 0 0 0 0 0 0 0 03 0 0 0 0 0 0 0 0 0 0 04 4 6 5 5 4 5 4 5 4 4 465 11 13 14 12 15 10 16 13 131 12

01 0 0 0 0 2 0 0 0 0 27 W16 20 19 14 18 20 F 1 17 18 177

TOW. 33 35 39 36 33 35 40 35 34 32 352

100%

7D A 5 6 7 is S T|I - - -- -0 - -0 0 -

21 0 0 0 0 0 0 0 0 0 0 02 0 0 0 0 0 0 0 0 0 0 04 4 6 6 4 5 5 4 5 6 3 30S 116 14 15 10 17 15 13 15 12 15 142

6 0 0 0 0 0 0 0 07 121 20 22 1 19 16 1i 20l 1 194

TOtW |41 40 43 33 41 3| 35 40 34 41 306

Control-2

SM 1 1 2 3 5 4 7 a 9 le1 0 0 0 0 0 0 0 0 0 0

2 0 0 0 0 0 0 0 o 0 0 03 0 0 0 0 -0 0T T T 0 0 04 4 5 5 5 4 6 T T 7 4 475 14 11 12 10 10 11 9 0 13 11 101' 0 0 0 0 0 0 130 0 137 19 19 13 20 1713 17 17 191 176

Ted| 34| 35 34 30 34 34 29 35 34 34 337

100i/ Intke- .-. a S - S - TOW.-

1 2 3 4 5 6 I7 a 9 TlteI 0 1 ° I o I o I o0 o l 0 ~1 0 0 0 0 0 0 0 0 0 0 03 000 0 0-0 0 00 04 4 -6 4 3 S 4 6 5 5 5 495 I15 17 13 1 12 13 15 0 13 16 115

1 0 0 0 1 0 317 120 21 16 19 20 16 21 11 18 20 189

TOW. 1 39 44 33 46 37 33I 4 39 34 41 3d4


Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0)Species: Ccriodaphnia dubla

Quality ControlVerification of Data Entry, Calculations, and Statistical Analyses

Clien TVI Seueyuh Nuclear Plant - Nonoveated

Teft datea July 7-14,204

Project nubern: 1321 Reted byO ______ __ ___10 6

R- --e ._ Snunival Average repMutlo C-Ddwf d Pee aruef -f

(%) 1 2 3 4 5 6 7 S 9 le () (Offa"fe"mae) I lb (%) POW gteg CA)

cntrol-I 35 35 35 33 32 3S 29 36 33 34 100 33.1 6.1 N_ tappicable

10."% 30 35 35 34 30 34 36 30 31 34 lo0 32 7.2 2.

22% 32 39 30 36 32 38 32 38 35 35 100 34.7 .9 -3.0

43.9% 32 35 34 39 41 36 39 32 34 35 lee 35.7 89

72% 33 35 39 36 33 35 40 35 34 32 100 35.2 73 4,5

100% 41 40 43 33 41 36 35 40 36 41 100 39.6 S.6 44

Control - 2 36 35 36 30 34 34 29 35 34 34 100 33.7 7.0 Not applcable

100%Intak. 39 44 33 40 37 33 42 39 36 41 100 384 9.5 -13.

Dwvmetta MSDvahe.

Dwmst MSD valuce

PMSD:

2742

7.1

MSD- bMinium SignifantDifferencePMSD - Prcentm Minimn Snficant iffaerce

PM SD is a easwereof fepercistpi Tb. P4SD is em m pecen diffrecn the cnitrol and W_ tmtnt tat ca be decared atatiaticalbyintficktin ha whole effleenjt 0nfty s.-O averge a ugwrfl tdiffamuneoocmu for Eivromnmtal Tening Solutioms In chonic todcity Wtea wen

a ocantnreucs CelrodaphWa reproductionby 10.1% from the control.Lower PMSD bound dee by USEP A (10* perentfte) - I 1%Upper PMSD bound dermed by USEPA (90 percentile) - 37%The lower and upper bounds were calculated by the USEPA osfng 393 tes conducted from 33 labotteres for Clodphenkreproduwton in chroncrdeence tndcantl

USEPA. 2000. Undebanding and Accounting fbr Method Varnbity in Whole Effluent Toxicity Applicaions Under the National Poltant Discdhge Eniination ProgranL EPA-833-R0OM03. US Environnental fPctionAgenc, Ccnai OI.


Statistical AnalysesCedodaphuIla Survival and Reoductio t-R

Start Date: 7i7/2004 Test 1D CdFRCR S ple MD : TVA I Sequoty Nulear Plant - OutfNll 101EBd Date: 7/1412004 lab 1D BTS-Bnvir. Testing SoL Sarple Tye: DMRI,- eige Monitoring lRepotSaune Date: Potocol CHRONl-EPA821-R.02-013 Test Species CDCeriodapia dubliCo menbt NON-TIATEAD

C;eu-% 1 2 3 4 5 6 7 a 9 10D-Control 35.000 35.000 35.000 33.000 32.000 35.000 29.000 36.000 33.000 34.000

10.98 30.000 35.000 35.000 34.000 30.000 34.000 36.000 30.000 31.000 34.00022 32.000 39.000 30.000 36.000 32.000 38.000 32.000 38.000 35.000 35.000

43.9 32.000 35.000 34.000 39.000 41.000 36.000 39.000 32.000 34.000 35.00072 33.000 35.000 39.000 36.000 33.000 35.000 40.000 35.000 34.000 32.000

100 ; 41.000 40.000 43.000 33.000 41.000 36.000 35.000 40.000 36.000 41.000

Transfrm : Untransfarmned 1-Tailed IsotonleCoeC-% Mean N-Mean Mean Mnn Max CV% N t-Stat Critical MmD Mean N-MeanD-Controi 33.700 1.0000 33.700 29.000 36.000 6.105 10 35.133 1.0000

10.98 32.900 0.9763 32.900 30.000 36.000 7.228 10 0.644 2.287 2.842 35.133 1.000022 34.700 1.0297 34.700 30.000 39.000 8.914 10 -0.804 2.287 2.842 35.133 1.0000

43.9 35.700 l.OS93 35.700 32.000 41.000 8.563 10 -1.609 2.287 2.842 35.133 1.000072 35.200 1.0445 35.200 32D000 40.000 7.311 10 -1.207 2.287 2.842 35.133 1.0000

100 38.600 1.1454 38.600 33.000 43.000 8.566 10 -3.942 2.287 2.842 35.133 1.0000

AvnlIry Tests Statistic Criticl Skew KIrtKoMhgmvD Tes indicates norm, distrlbunon (p > 0.01) 0.73571324 1.035 -0.0294286 .0.8460054Bartlett's Tes indites eqmd variances (p - 0.74) 2.73092866 15.0862722HNOhe-s Test (1-tal, O.05 NOW hVDEC CllV T MSDt MSIp M8B MSE F-Prob dfDunnetits Tes 100 >100 1 2.84245189 0.08434575 39.1466667 7.72592593 7.0E-04 5,54Treatmets va DwControl

sqn_07-07-04data


Statistical AnalysesU bzwphThx (2Op Remd_)

MMwR FD 9% CL

i005

r0XROlaOX=#

mEn0

>.100 I

>100>100

>100

O.L

0.8

0.3

0.1

0.0.0.1-2A -.0,0

- b . t @

.............................

0 20 40 0o so 100 120

Dome %

-R_ nut

45.

40

35.

g 30.

a8201 5

Is

10

S

0o

.I-= I 14.1,0.05 Iveofewfldm

I di '.4

S.-

qn_p7-O7-X4data

TVA / Sequoyah Nuclear Plant - IntakeNon-treated


Cnlodsphnia Survival mnd Reprodyetion Te t--Reprodve~Stat Date: 7t7/2004 Ted D.l CdMRCR Sanupbl D: A I Sequsy Nucler Plant - IntakeEnd Date: 7/142004 Lab MD IB-Envr. Testing SoL Savle Type: DMR-Disohgp Monitong Report

Sa&mV Date: Protol: CHRON I-EPA-821-R-02.013 Test Species CD-Ceriodaplnia dubiaConments. NON-TIhAThD

Cone-% 1 2 3 4 5 6 7 8 9 10

D><antrol 36.000 35.000 36.000 30.000 34.000 34.000 29.000 35.000 34.000 34.000100 39.000 44.000 33.000 40.000 37.000 33.000 42.000 39.000 36.000 41.000

Transfom: -UntransformedCone-% Mean 1-Mean Man Min Max CV% N

_

_

1-Tolledt-Stat criticl MIM

Isntonl-Mean N M.

D-Cbntrol 33.700 1.0000 33.700 29.000 36.000 7.001 10 36.050 1.0000

100 38.400 1.1395 38.400 33.000 44.000 9.525 10 -3.415 1.734 2.387 36.050 1.0000

Avm!r Tesht statismf CQicel Skew Kurt

Shapiro-Wik Teot idicates nmnml distribution (p > 0.01) 0.93153244 0.868 -0.4628473 -0.2391617

F-Teo hyficates equal vinces (p 0.21) 2.40319371 6.54108953Hypothesis Test 0.0) MSfu MSB MS -F-Prb df

Homooedastic t Tesd indicates no significant differences 2.38674469 0.07082328 110.45 9.47222222 0.00308902 1, 18Treatments vs D-Control

sqnO7-07-O4data

TVA / Sequoyah Nuclear Plant - IntakeNon-treated

Statistcal Analyes

obwPrM % RD n% CLICs

l106T2S

Tr

>100

>100

>100>100>1o0

>100

*I.g I

0.90.8A.7

[0.6

a OA*

0.2Q.1-nnl -

-Q1*.0Z r.

0 20 40 60 so 100 120

DM*

Dmus-Rexp~Met

�qj j

ff

40

35

030~

16

10

5.

I......................................................................I I 14b.60.05 kemOfstnleance

WI.

Isqn O7O07-04data

Envlronmental Testing Solutions. Inc. Page I of 6F�nvironmental Testin! Solutions. Inc. Page bE 6

Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0)Species: Pimephalespromelas

Client: TVAFacility: Seguovah Nuclear Plant - UV-treatedNPDES #: TN 0026450Project#: 13?ZI

Dfludon preparation Information: Comments:'Dilution pe (%) | 10.9S | 22 1 43.9 721 100 r-tAl 5 4AUP -A&fiArrOTJAJ1JAPS45

Effihent vWlume (mL) 274.5 f 550 1097.5 1800 250 IT L 2A9tuIDluevlz (iL) 2225.5 1950 1402.5 70 0 tn O Q A l C

Total volume (mL) 2500 2500 2500 2500 200 rreA e e s,

Test organIsm information: Test lnformation. |Organism age: 21S.fl rs mo Randomizing template: RDate and times organisms C-o6-tq %bctl ' %S 4tT Incubator number:were born between: . , _ __

Organism source: AS_ 6 IS 61-M'4* Anrtemia lot number. BberlS5Transfer bowl information: pH Temperature - 'c Total drying time:

1.Ss Zq.S Date/Time in: av-i4 IAverage transfer volume: Date / Time out al 1fl ait 0 v

* Oven temperature: V'C

Dailyfeeding and renewal Information:

Day Date Morning Afternoon Test Initiation, Control water Sample numbers Analystfeeding feeding time renewal, or batch used used

- time termination time

I 0-4- *O 0&'Sb _lSC> 113SOM 01-%O>LbJ 04CU.04,3;o2 ____-_ 15 s6 O1-btoW0 4 0 00, I

3 101.1o ONI AS6 I -a&3 tn.A-Oq Oq 01'-.-02-I1 .07 At4. Dn. 110 _ _ _q_ O 3. 07-otc4 4O o.o 1.I6

65c 07. is rss4 IS _ ____6 ___ _ e Io. 0 -

0-4 B. O&$ t6 I's5 -Zs 144M 10n 10 *el/Ai KEl7 __I;I2v .@. .X :'3t I.,- A -I-

Control Information: | Accptncenti Summaryof testendpoints:% Mortai . Z.S7 l s20% V 7-day LCm _ _I

Average weight per initial larvae: 6. 0S5 . 3 NOEC _____

Average weight Per surviving larvae: 0.112: 2 0.25 LOEC ) I mfl

ChVIC25 -- )I 7.

Environmental TestinL Solutions. Inc. Page 2 of 6Environmental Testln� Solutions. Inc. Paze2of6


Client: TVA I Sequoyah Nuclear Plant - UV-treated

Date: 01-04C

SurvI 0and GrowthData _

Day CONTROL _ 10.98% 22%A B C D E F G H I J K L

0 /0 /0 /o1o1 10 10 ,0 10 to to

/0 /0 /C 1 ) _ 10/0 - - to -/ /O

2 IC C > lo CIL) 10 lb I 0 /O /0 i) to

/D/ /0 /t /0 to /0/0 /0 /t /0 /0

/0/0/0 10 / 0 _ /C)/0 -/ 10 / to

s_0 g) 10 V 10 /d d0 /6

6 -0 -t -°-/ - °/ t t{)1lo______ lo / l o lo010/0 /0 /0 /0/to 10

_ _1_ IL) 9 q o #0 JO , toIO 10 10 10A-Pan weight (mg)

B - Pan + Larvae weight

Lare weight (mg) - A - B 2 1 pa s _ e<: ,¢ * \'

Weight per iultal number or A 4

- Cl Inltal numberoflarvae O b 0- O- 6 -Average weight per Initialnumber oflarvae (mg) 0. 0. L, 0.101

Calculations and data iewed:4

Comments:

Environmental Testing Solutions. Inc. Page 3 of 6Environmental Testin! Solutions, Inc. Page 3 of 6

Species: Pimephaespromdas

Client: TVA I Sequoyah Nuclear Plant - UV-treated Date: o01-01oct

SurlsvaI and Growth DataDay 43 % 72% l-1%

__ _ MN 0P R S T U ---

0 /0 lo 10 l0o / to 10 1o /o 10 10 /OI__ __ ID /O /O o rIC)to O oto /0/10 to

2 I'D lbD 10 /t la lI /D -0//6/C) /O

3 /0 D /O O _ O / tot /6 104___ _ //0 / /0 /0/0/O _0 _0 / O / 0

_ _ _ 10o la0 Idoq 1') /O-° 1 o lo6 I / /6 100 to " 6 to _ / 1./0 _c1

IL_ ) lo lo0) _ J lo io 0J A/OA - Pan weight (mg) _ , _ _

B - Pan + Larae weight

____ __ _ _ _V~ __ _~ _ _(Rig)tperlultlalaumnberef A a aJA.Larnce weight (md ) A - B +U'% o tw> /5>5 n 8

.CIIltfal number of rae Q, O _ O ___ O' C) O _Average weight per initial _ _number of lme (mug) O.Ls

alculations and data reviewed:

Comments:

,f��

I....Environmental Testing Solutions, Inc. Page 4 of 6-


Client: TVA / Sequoyah Nuclear Plant - U-treated Date: C -O'?-c

Survival and Growtk DataDay I- 100% Intake -

Y Z AA BB

0 10 10 /0 /0

o( tob t0 /

S0 (_ /6 /

6 IC ) ;R 107_

_ _ _ _ _ _ _ _ _ _l a 1 0 g0& /A - Pan weigbt (mg)

B -rPan + Larvae weight

Larvaewelgbt((mg)-A-B B

U .__ _ _ _ _ 4 ,. ; ,

Weight per Initial number of A * - -

lame (mig) e- C I nitial number oflamse 'QAverage weight per Iltialnumber of larve (ng) 0. 6O6tv

Calculations and data reviewed:

'Comments.-

TVA I Sequoyah Nuclear Plant - Outfall 101U -treated

Chronic Whwe Effluent Tor"ity Test (EFA-U21-R013, Method 1000)Speder- Pmephakuspremda

Quality ControlVedtfleatlo f Data Entry, Calculations, and Statistkal Analye

JAIlqm rstVf

Te aft m.1 aty

h I 2

2006A

Rm I " " b r _-Am k 4 9 �_ - - ^- . - -tL-- -S-"opns -- U *,qEmmIm bagd 0U-mp-"~ acb.) smmbs Twbkt~wd ind (V.)

A 30 10 15.04 275 7.71 0.771 o.a 10 10 14.73 21.01 6.23 0.6207 3 5 IH 3eC to 9 35.00 21.07 0.61 "4 0.607 10.51* 04apa3D 10 10 14.5 22.33 7.75 0.775 0.775E to 10 10 34. 21.40 664 0O44 0.A44

F 10 t0 15.01 22.02 701 0.701 Q701to t0 14.70 21.65 695 0.65 0.01 1A" s sn . 10 le 14JS 20.6 S.06 _OA6 Oe60

I 1 15.07 2L46 7.39 Q739 o0.739

J2 o 10 30 15.02 -22.30 7.23 0.7213 0.707 GA 0.723 O .07G LK 10 10 14s4 21.24 6.0 0640 1 07 66 -L7

L t0 10 SAS 7t L_0 t22 0Q722 Q

M . 10 10 15.16 23. 0 7J4 0.747

74 10 30 15.02 2L. 6.67 tAll A6m7 .0 10 14A2 3.03 6A= 0.661 t0.61r 10 10 14.64 2IA9 6.5 0.635 0.635= 10 10 15.00 21.41 64 0.41 0.641

72% R 30 o9 1497 21.39 62 0.713 Gm 4. 0642 97 6A67* 4. 4.1

5 toto -4.7 21.7 702 07020.702T 10 10 14.70 21.52 6J2 Q652 0.62 _

U 10 30 14.60 22.5 791 0.791 0.791

10% v 10 30 15.33 23.29 0.796 0.755 6.0 0.796 133 40. Le 4.6W 10 30 14.72 22.0 .2 0.723 0.728X 0 10 . 14.74 210 0706 0.706 - .

t o lo 14.43 213 67 0fi7 efi7IOM z 30 10 14391 22.35 7.24 0.724 0.10.724 .63657

AA__ 33 30 14.7 23.32 .56 0I65O 0_91 _ 0 0.656 "A S 1- -

BB to It 14.76 21.32 6 X OAX oA|6

Qah" 1M1DemeiaSDwIme:

webshowmgnopMSDO

11.694126

U.12236.3

POD- Mh ~~iSDIlecFM.D ?eft bfibsma~dmluetDiffeuw

awea_,lmld_ dtfieum hcawrciiaTua Sand Iuebee..hddyeeuhatocawuupsAu awnb 34.% Doe eI(dei..dae

LowerPMSDbo_ _wAee **m0) -9Uppr9UDi dpe00 tUMEpA(0&V-snfl)-35S%albee uduyapusa s we eale4 bWy the USeP les k 205 Add _l u ml ow meke bWhetucief

USWA. 200 aunt m Ao t Mei Vo Wole E Ap U u Do E EPA.334.003. US w a o Alm A hed. 01.

TVA / Sequoyah Nuclear Plant - Outfall 101UV-treated


Larval Fish Growth and Survival Test-7 Day GrowthStat Date: 772004 Test 11 PpFRCR Smple Dt TVA / Seqioyh Nuclear Plant - Outfall 101End Date: 7/14/2004 Lab mD: ET-nvir. Testing Sol SMplo Type: DMR-Dirge Monitorg R rtSample Dete: Piutool: CHRONIC-EPA-821-R-02-013 Test Species: PP4' aes pmelasCm :miat WV-TEATD.

Come- 1 2 3 4D-Contral 0.7710 0.6280 0.6070 0.7750

10.98 0.6440 0.7010 0.6950 0.606022 0.7390 0.7280 0.6400 0.7220

43.9 0.7840 0.6670 0,6610 0.685072 0.6410 0.6420 0.7020 0.6820

100 0.7910 0.7960 0.7280 0.7060

-

Transform: UntransformedCOW% Mean N-Mean Mean Mh Max CV% ND-Contral 0.6953 1.0000 0.6953 0.6070 0.7750 12.974 4

1O.98 0.6615 0.9515 0.6615 0.6060 0.7010 6.799 422 0.7073 1.0173 0.7073 0.6400 0.7390 6.417 4

43.9 0.6993 1.0058 0.6993 0.6610 0.7840 8.211 472 0.6668 0.9590 0.6668 0.6410 0.7020 4.542 4

100 0.7553 1.0863 0.7553 0.7060 0.7960 5.974 4

I-TOt-Stat CrM4

0.860-0.306.0.1020.726

-1.529

ifed Iotolcicad MSD Mean N-MeOn

0.6975 1.00002.410 0.0945 0.6975 1.00002.410 0.0945. 0.6975 1.00002.410 0.0945 0.6975 1.00002.410 0.0945 0.6975 1.00002.410 0.0945 0.6975 1.0000

Auli Test statistic Critieal Skew KurtShepiro-Wilkb Te indiates noml distibtion (p > 0.01) 0.95757478 0.884 0.0475234 .0.9287226BartlettI Teat indicates equal vrianc (p = 0.59) 3.73211074 15.0862722HpThesis Test (1-tal, O.O) NORC LOEC ChV TU MS~u MSDp MSB MSI F-Prob dfDumnett's Test 100 >100 1 0.09454345 0.13598483 0.00454384 0.00307793 0.2462927 5, 18Treatmnts " DiControl

sqn_07-07-O4data

TVA / Sequoyah Nuclear Plant - Outfall 101UV-treated

Statistical AnalysesxJX,,w-bXeem(2"0 Resuiyhs)

1 an 593% CAEx1)_ _ __ _ __ -s _ .

ICIO

IC'Sid20RU25

IC30nco

>100>100>100>100

>100>100>100

- i.0 .a...

0.7

as Q5

! 0.4 'Q46

0.20.1

ULU - - - --. e - -- - - e

-Qt

... ,

0 20 40 80 80 100Dote %

120

Imt-R-pm

0.9 -

0.7

p?0.4,0

Q.2

0.3

............. .. ......................................................... 1-ut 0.0SofugimlN

n.

2

sqn07.07-OMdata

TVA / Sequoyah Nuclear Plant - IntakeUV-treated


Larval Fsh Grwth and Survvl Tet-7 Da GrowthStal D&te 7/7/2004 Test lD PpFRCR Smle ID, TVA / Sequph Nuclear Plant - Intake

End Dat: 7/4004 Lab ID ETSnvir. Testing SoL. Sanle Type: DMR-Di shap Monitodng RepodSwnph Date: PmooL CHRONC-EPA-821-R.0o2013 Teat Spesx PP-Pimophales pomelasCommentE V-TPATED

CGn 1 2 3 4D4Contol 0.7710 0.62R0 0.6070 0.7750

100 0.6870 0.7240 0.5560 0.6560

-

Transform: UntransformedConM-% Mean N-Mean Mean Mil Mal CV% NDwContn 0.6953 1.0000 0.6953 0.6070 0.7750 12.974 4

100 0.6558 0.9432 0.6558 0.5560 0.7240 10.991 . 4

1-Tailed Isetnoct-Stat Citical MSD Mean N-Mean

0.6953 1.00000.684 1.943 0.1122 0.6558 0.9432

Aurdiary Teds Statistie Citical Skew KurtShapiro-lVfk Tet idcates nmrn diftribution (p > 0.01) 0.86392242 0.749 40.3059472 -1.9996062F-Tes incates eqmul vmices (p - 0.72) 1.56619453 47.4672279Hypothesis Test O1.Msl 05)MSfu MSIpp MSBI MSE F-Prob dfHonwaoedastio t Tet indicates no significant differences. 0.11218045 0.16135268 0.0031205 0.00666558 0.51937759 1,6

Treatents vs D.CcotnW

sqnO7-07-04data

TVA / Sequoyah Nuclear Plant - IntakeUV-treated

Statistical AnalysesMWd RHe

ridt % mD 9% CIAM"ie1D5*

IC10

r:20=0rAOW!o

MON>100>100>100>100>100>100

in -.

*oivtl B fin lk s cO. lowes 0@ Usc G us

0.9'

0.7

E QE0.6-

S n

0.3-

OL2-

M.1

nn -...- .

0 20 40 60 O0 100 120Dose %

U.9 .0.9

0.7

80.6

tQ4

0.2

01

of s8gnifican

n.Ii

sqn_07-07.04datb

Environmental Testing Solutions, Inc.

Chronic Whole Effluent Toxdcty Test (EPA-821-R-02-013, Method 1000.0)Species: Pimephalespromelas

Daily Chemical Analyses

TVA/Se hNwdewrbd, Not' ed

Test dr. Jly 97 14 20

P 3ectnmwer 1321--

-

I d6.1 k ToI a To.UL- I TL_. I

F-cefteo ign

Cn UIS

C-DO (Mi/)

4-COuN&WTjfty (H~nbsdfln)

cam" - .. 'U-em- ong IC&

A v _

-4- S

rffinewaeMs enx4.�777 -' 4�- I-�--$ -t -�t �!I !ton min

;N , -- -, ,nDb il)10.8%

rfa.. u f (- - l - -T C) 25.2 249 25.11 24.81 25.21 24.5 25.2 243 25.41 2421 25.11 24.41 24.5 24.1

wH 7.64 72 758 7.81 8.04 7.81 8.16 7.74 8.02 8.06 8.16 8.02 1.22 7.8122 = 7.8 7.5 7.6 731 7.61 6.9 7.8 6.7 8.0 7.91 7.61 8.1 7.5

22% C ( _) 246 254 228 280 274

_ _ _ m 252 247 25.1 24.7 253 24.5 25.1 24.1 252 24. 25.1 24. 24.5 24.1v1_ 7.63 7.7 7.55 7.8 8.03 7.85 8.15 7.80 7.96 8.0 8.15 8 8.19 7.79

D ni+L) 7.8 7A 7.6 7 7.6 6.9 7.9 6.8 79 .7 8.0 7 82 734&9* 2~ 381 24 2 - -24z5a Z43 261 255

25.0 24.7 25.0 24.7 253 242 253 242 25.2 2.2 253 24s 244 2437.60 732 7.54 75 8.02 7.86 8.16 7.83 7.91 8.14 8.04 8.20 7.82

7.8 7.5 7.6 72. 7.7 6. 7.9 68 -8.0 8.1 7.6 8.2 7.4729 i t ~*t~l6"nwd~n) -214 2Z0 - 2200 22 220 236 233S

T C) 25.1 24.6 25.2 24.8 25.4 ?4. 25.1 242 25.2 24 25.1 24 24.4 24.07.591 7.33 7.49 7.90 7.99 7.96 8.14 8.01 7.84 8.16 1.13 8.12 8.18 7.9

7DF.L) 7.8 7.5 7.6 7.2 7.6 7.0 8.0 7.4 7.8 7.8 8.1 7.7 82 739=kn =!fLM~es d19 -191120 19' 5 2r

100 OBLCKW 781 g

TOW ReddNl aCwhsne O"A.1)TIpewrprnC) 25.0 24.6 252 24. 8 25.5l 24.5 2 25.1 24.0 25.1 243 243 2PR (U) 7.671 7331 7SS551 t 8.121- 793.0 .91 .541 8.171 8.361 8.141 8.471 .6DO t ) 7.91 7.51 77 . 7.81 7.01 82 . 8.71 7.91 S.11 7.71 8.3 7gn±1563t !!2_ 197 i 19415 0

100% bJune Afkfbtty (ni/L COC0) 79 81R__ _On 9t0L 90 9R2 1

Tea ReuInduae Q </L) O.

Te-pwtms (C) 2531 24.91 25.0 24.7 25.21 24.51 25225.1 2431 25.2 2431 24.71 24.1

I ILI.

Environmc

I ' Spec

Daff

I=Conce

I coi

I

.ntal Testing Solutlons, Inc. Page 5 of 6

les: Pimepholspromelasit: TVA / Sequoyah Nuclear Plant - Non-treated

Chaemst:

Date: 6 O1.oa

I ICAwFI4P^s~

I Day II 0 I 1 I 2 I

alyst

ertrtioo IParameterFTROLI pH (S.U.)

_~~~ _ _ _- _ I.rDO (m/L)

r --

Conductivity(umhos/cim),=_ _ _Alkalinity(ma CaCOL)z �v �-� � ir -Hardness(me CaCO,/L)x--D Z _E

Temperature4. -

pH (S.U.)10.98% DO (mg/L)

ConductivitytuFmhos/cm1

Temperature (C

pH (S.U.)22% DO (mn/L)

Conductivityflmh/em

Temoerature (°C)

pH (S.U.)43.9% DO (mgtL)

Conductivity(pnhos/cm)Temperature (C)

oH (S.U.172% DO (mmIL) T. B

Conductivity(Amhoslem) I L1qTemerature (C)

4 .9' ----

pH (S.U.) .51 s I100%

*

DO (mg1L) _Conductivity 2(Amhoslcm)Alkalinity 7¢(mg Ca4COYL)

Hardness e(mg CaCO3/L)

TR chlorine

(mg/L1 0.10

_w.

- 2S0 1-

I _

\ L

2S.t _

2A.L6 ZS-

'fs l

214 ZS. 3 'Z

6.S t.cz -q

14.2 7.6 _

2A m

24.-4 _5.3 _

If4 8.tY I

Temnerature I'C) 75.0,nT {s ii V:_-. . _-- - 17 fl I I

_ 5100% Intake DO (mOIL) if.). lef

Conductivity . .(ga1.(wnhcm) ' 1 I t 1 .Alkalinity 4(mg CaC03/L)Hardness O(mg C8CO3 /L) . 0TR chlorine ZD. ISk

(Mg__

_ 1

. = F4

_ .4-4 Zi2 _Temnersture (°C) I . Z5*U 9:m. - -

Intial _ i- . Initial I -Fina-Initial I Floal- � - h - -

r�"_T_7- '11,, , ., :., .7

I Environmental Tesdng Solutions, Inc. Page 6 of 6,

Species: PimephakspromekzsClient: TVA / Sequoyah Nuclear Plant - Non-treated

Date: la-.C4 14

II Day I

I 3 I 4 I S

4I -- , rConcen- Parametertraton

'ICONTROL 1pH (SMU)

DO (mg/L)Conductivity(umhos/cml- \ I

Alkalinity(mr CaCOIL)-- 4 -I

Hardness1wc C&COJL)t~~ -_ _, ,.Teanerature (MC)

I II - -

OH (S.U.)10.98% DO (mgWLU

Conductivity(umhos/cm)Temperature (MC)

pH (S.U.)22% DO (ml/L)

Conductivity(uimhasdm)Temperature (CpH MS.UM.

43.9% DO (mk/L)Conductivityformhtm~rm)

Temperature.U IC t1f

72% DO (mg/) I ., eConductivity _(pimhos/cm) I I

- -- 1

8.l ,*.Ii-~ -2 3 M-

Temnerature ('Cl ZT2s.7 7S.1 2'. a Zq.OI-- -

pH (S.U.) (A.14

100% DO L(n^)j | 6.0Conductivity(pmhoscm) ZOOAlkalinity(mg COCO3AL)Hardness

TR Oiorlone (nmgIL)Temnerature _, -

pH (S.U.) 3.ZO100% DO (MgL) 8.4.Intake O_ _ _ _ ._._

Conductivity qq(pwcyn)I tAlkalinity(mg CaCOL)Hardness(mgCaCO 1/L)TR chlorine (fmgL)Temperature ("C) IS. .

Initial I


Chronic Whole Effluent Toidelty Test (EPA-821-R-02.013, Method 1002.0)Species: Ceriodaphnia dubia


cmlent TVA /SeqOe IN

Tat datw. Jl 07 -14, 204

hPchT ]M, Nontrmfted

-

"jetW vmba 1321 Rerk t rdyby- 9 fi&2!.* .-I -

csaCtsom

I - - t.

D ayl I Day 2 | D "3 | DRy4 1

41 741 8.09 8.091 8.12 8.21 8.23 8.1576 7.8 7.6 7.8 7.7 7.8 7.9 7.7 7.8

11I IstI

DO (mi/L) 7-9Tfndutmtvftim Wa-rM)Albifty (*WL CRCO,)fflsn(mwLCwC03) I

C)e C 25.4 24.9 25.1 24.8 25.2 , 24.9 25.2 24.7 24.9 24.6 2.0 24.7 245 25.2pH (SU) 7.65 7.46 7.581 8.06 8.0 8.11 8.15 8.21 8.08 8.16 8.17 8.24 8.23 8.04

M1 *% DO hML) 7.7 7.5 7.6 7.6 7.6 7.8 7.9 7.9 8.1 8.1 7.9Cebdwedy" 2U 261 266 28E 268 287 2 8 1 ~_repe C) 25.2 24.9 25.1 24.8 25.2 24.9 25.0 24.7 24.8 4.6 24.9 24.7 24.4 25.1pH(SU) 7.64 7.47 7.58 8.07 8.04 8.12 8.16 8.2 8.02 8.16 8.16 8.27 8.22 8.05Do (DO L L) 7.8 75 7.6 7.7 7.6 .7 f 8 7. 8.0 7.8 7.9 8.1 8.1 7.8

22% _ _m) 246 25 261 26E 5E 280 274

Temp e ( 25.2 24.9 25.0 24 25.2 24.9 255.0 24.71 24.8 24.7 24.7 24.5 253Oa11) 7.63 7.47 7.55 8. 08.0 8.13 8.15 8.22 7.96 8.19 8.15 8. 8.19 8.0

DO (mg/L)7. J 7.8 7.' 7.6 7.6 8 7.9 8.0 7.9 8 .2843%238 _2; _244 _ 250 243 261 _25 _

T% -h"e C 25.0 24.9 25.0 24.9 25.3 24.8 25.1 24.7 24.9 4.7 24.7 24.4 252I _(__ 7.60 7.54 8.10 8.02 8.19 8.16 8.27 7.91 8.23 8.14 8132 8.2012

72% Oi Lu) 7.8 7.5 7.6 7 7.7 7.8 7.9 8.0 8. 7.8 8.1 8.2 7.8ondu""I b ) 214_22(0 221 _ 221 _ 220 236 _233

___ _ CC) 24.9 25.0 25.2 2 25.3 24.8 25.1 24.5 25.1 24.6 24.7 24.6 25.3H(SU) 7.59 7.51 7.49 8.1 7.99 8.23 8.14 8.29 7.8 8. 8.13 834 8.18 8.14

DO (mdL) 7.81 7.5 7. 6 7.6 8.01 8.1 7.8 7.8 8.1 8.21 7.9. 1I~tmmLm 204 _12_1 0 9 1 0

100% ALkal CRt) 77 71 _ 77dn (Mg/L CC) 388 8E 90|

T&t RaldeaChline (n wL) . 0 ._ . 0 cO.10La"____ != CC -- 25.01 24.81 24.91 24.81 25A 24.9 253 4. 2.14. 2.447 2.753pRl(SU) __DO SML 7.67 7.4 7.551 8.13 8.121 82.0 82 85.14 824 84.31 84.33 84.471 2.11Do('L) 7.91 7.51 7.71 7.6 7.8 8.01 8.2 7.8 8.7 7.71 8.1 8.11 8.3 7.9

100% Intel Alality (n/L CpCO,) 79 8flrdM (M9LC" 3 90902Total Refdm rle (wiL) <.

_ _ T"peatur _c) 25.31 24.81 24.9 24.9 25.01 24.8 Z5.1 24.7 25.0. 246 24.6 24.7 25.0 253

Environmental Testing Solutions. Inc. Page 6 of 7Environmental Testin! Solutions. Inc. Page 6 of?

Species: Ceriodaphnaa dubtaClient: Seauovah Nuclear Plant - Non-treated

DaftA ChenLtry:

Date: O4 ' °. Co

d t f A "aROcs)i~~0 (eese o fe-Day

I _-= . _ _ _ 2Analyst bs I 7c8 IYCD IY' B I t.6 U

Concentration ParameterCONTROL OH (S.U.) 1A5 &vd ?A11 .1 1 ILM I $. I Ai

4 - 2�� IDO (mgVL) ,I.., IConductivity Z(gmhos/cm) _ _3

Alkalinity(mg CaOO3/L) (elHardness(mgCaC03 /L) I

If \

N. -_2s.t1_ -M

I

6.d

Temnerature ICpH (S.U.) .65

10.98% DO (nmAL) -IConductivity(nmhos/cm)Temperature("C) 2ZpH (S.U.) 7. EA

22% DO (mg/L) SConductivity(Irnmhos/cm)Temperature1°C) 25 .

_ .6 7.6 7.6*

i 26 e* 8.01 2S. I z.BZ 5Zs.Z

= w.s M &V 0.047.6

Z 942S.O

_ .6

_ zzI

- I

I Zy4pH (S.U.) ?. 6E = I.sST j ECs 1 GV - B.1_ _ _

43.9% DO (mWL) _ .

Conductivity 1 39 2s0(;Lmho I - MN ZiI .6 iI 2Y.

Bl91Temperature MC) 7.s. 0a - - - -b.pH (S.U.) . 4o 7.}s

72% DO (mg/L) 7.

Conductivity 2*1(Pimhos/r) 2Temperature (QC) 2qw.

'S 9��pH (S.U.) I.514 - -�

100% DO (mglL)

4

Conductivity Zoq(Pmbos/cm)Alkalinity(mg CaCO3lL) _7___

Hardness 6(mg C8C03/L)TR chlorine_mAL _ _ __

_ .7

Is wZZZ53

= W~tI V.,6

M Jq

'1 451 85

I 40.10_ Asn

2.8

Temperature (°C) ZS. UpH (S.U.) I . Vf

100% Intake DO (mgfL) ).tConductivity

Alkalinity.(mR CaCO/L) 9Hardness q g(mg CaCO1/LPTR chlorine -I F

a Temperature (C) I 3I

S.Iz

14.4!I i i

initialfinInitial I I

Environmental Testins! Solutions. Inc. Page 7 of 7Eniomna _ etn!Sltos n.Pg of 7

Species: Ccrlodaphnia dublaClient: Seauovah Nuclear Plant - Non-treated Date: WI.DJ1.-b

U

I Day III .3 I 4 I

Analyst_Concen- Parameter etntlon 1I

CONTROL pH (S.U.)DO (mgIL)Conductivity(p~nhos/cm)_Alkalinity(mg CaCO3/L)Hardness(mg CaODVL)Temperature (MC)

10.98% DO (mA/) I 6 - -V - +q9

Conductivity Iv 9ITemperature M') 25.0 9 Ii

*1,W .

Ip

ie

\2.

6a.1

Ze. C

B7P

PH (S.U.) e-.to A It.3- - - -

22% DO (mg/L) 8.1 142Conductivity zco -M a i I Zi14Temperature (C) -2.S .0 ZWXpH (S.U.) B is a

43.9% DO (mg/L) _ .9Conductivity mm(pLmhos/cmn)vTemperature ('C) .pH (S.U.) ex" 1 .2 q i

72% DO(mg/L) W .E O - 6OnConductivity

Temperature 'C) 2JS.

pH (S.U.)100% DO (mg/L)

Coductivity(iunhos/mi) ZooAlkalinity

(tgCBC03/L)Hardness \ 4(mg CBC03/L)

TR Chlorine (mgL)Temperature('C) .-3 VJ 1 Z 3pH (S.U.) ism

100% DO (mAL)intake

Conductivity(ymhocm) IqI9 Li

Alkalinity(mg CaCO3BL)Hardness

TR cklorine (mglL) .10OTemperature (C) _ZS. * 7

Z' .4 . 19 . _ _ _ _ _ _ f l. 3q

_._ fi _ __ | _ * g

1.1 I R.-I -i a

als

FM=

_" Nf-ZT. L_%.

7'I4.1_- -. ,-5-u I Zb..

I F11--iInItW us,1nitial Msa1 Initlal Iia Initial Final- _ a


Chronic Whole Eluent Toxicity Test (1PA-821-R-02-013, Method 1000.0)Species: Pimephalespromelas


CWLe TVA

Tatidaft: July

NoPe mmike 1321

I ShN"ader t, UVN.kTd

07-14.24

-_ IDV Fn I D" 1IF I jd"a. II.

'.55 7.28 7.441 7.78 8.091 7.777.81 7.5 7.6 7A 7.7 7.01

,H (iSLL _

DO (mrlL)

C- fl-e~Arb hMLNw wS

[frdRenXCA.%25.21 25.11 25.01 25.01 2531 24A1 2531 24.2 25.41 24.2 25.11 24. 24.51 24.2.. ...... 7 .q1 -1 d 7 7 .0.1 7 - a ..- taA. A a tap 7i1 S1 . I,,H (HU) |141 ,1,. 11 .4jI 1.1l 1-01 1.101 .YOI I.11 f.I o.wI o.v: D.&ol I."F Ob.,1 1.01

10.%I 7.8! 731 7.61 721 7.71 7.91 7.81 6.91 7.71 8.01 8.11 72 8.41 7.2= 23 260 270 277273 291 28 283

r_ _ do^ 25.4 Al 5.q11 27.11 25.1- 25.11 24.2 25.21 24.2 25.01 24A 24.61 24.'| fnn-

nut % . -- , _ _-71 7 - 7______________ 7.4 .2 741.5

vn0 £u.fl. R.0 7.3 7.6 7.322% - t-�iini .

S ,,,_.^_\

iwu~y~ liuru : ~ . -. 5 I ITrm.4me 1f* 2S.41 25.01 25.11 25.1pH (SU) 7.531 733 7.441 7.85

4DO nL) 7.8 7 - 7.7 7.3c4mdwlm (% l) 254j_--I:25.01 237

Temp"n' CD __ 253 - 25.2 . 25.0.. 1 -.. -. df i7 7d

8.111 7.76 8.031 8.06 8.16 8.0 8.24 7.797.8 6.7 7.8 8.0 8.0 7.7 8 .4 72

269 261 283 27925.! 24.! 25.2 24 25.1 24. 24.6 24.38.10 7.76 8.02 8.07 8.17 8.0 8.27 7

7.8 6.7 7.8 80 8.1 7. 8A 72249 2*4 265 211

25.2 241 25.2 24.0 25.1 24.' 24.5 24A8.05 7.95 7.99 8.14 8.18 8.08 8.25 7.787.9 6.9 7.7 8.0 8.1 7.6 8.4 772

2220 23 235

"H ENI 1 -1.3 1 J 1.1* ."I ,.71

72%DO (m74L)- 7.7 7.'1codfu~y (nbem)h 228 2138

ITeffmverture-Or

^ l

.. I Wil2i.1 25.27.3 7.44

7.6 7.7

25.07.88

2SA1 24.18.07 7.91 7.94 8.13 8.19 8.09 _

25.01 24.2 25.21 24A 24.37.89-1-1J

,..MI iO Of M7f a I

100%A02fl (I) CC

T9RedwCb] ln(mL)

DO (su/) I

hdkifit aceC)-100% Wk

Solutions. Inc. Page S of 6.- - -

Xc-ei-^Ziiiephdispromelasientr TYS I Sequoyah Nuclear Plant - Vtreated

Date: C R - 07f * oAey f A4, fai\ ewe

. I

- -

w

\ (exceed A" {q And

I Day I

I 0 1 11--x -11 -as n own oal 0A --A |

Analyst I

ParameterAla w w-^

s

E:,^-> -i!-';

St.'"",'''',e''.s,A,''',

An-.'.,% 1.,,An;-',I':',act: *;

.tt .: . ' - .

At

'_'-i-t, . . .

JW

'}ids fA.,.{-{. :

,$.s,: *

C':'*A..I: i I

At. '-' , ':<.';e .

A....r...',,

A;;! ,>

I Jo

:S.;,-..

bp

at

An'

J.i

i�"IA f

i-. :,

.K,

�tl

I� r

pH (S.U.)DO (mglL)Conduletivltyhtnnhnndem%

r. __

--AAnd He - l

M= zipS 1 w.

AHainets m lG R S \;

Hardnessn l, E

er0s -S& f - I WJ- la I -- a I

:;, i.}(S.U.) If5 AO 14AS F.76 qn.7e

*r;E., A-'S',Couductiviqty ~ ~

Temperatore (°C) 2SA t t5. 0 S 25- g. 1 Z45 3 ZA.7-

O. : H (SMU) ML .; 5,} ~Q .s A

-22%139e DO (mgL 7.> 7 477. .t t

- . . . ~Conductivlty 5 g2 B

; _Tempereiurt (OC) Z5A 2S S 3 Z5 0 Z,

2 . 7 ~~~pH (S.U.) 7}5 .jy X,5 ,sz 6 X R~

.- 3.972 DO (mg/L) 7S . t77.'s t o1

.; . ~~~Conductivity2 3 l 0

.Temper ture (eC) 2,351SZs.O Z5. 2 Zl*

pH(.U)TA .4 A 5$6 I

OR (S.U.) Y. $7.9 I sq

ISO% DO0(mgAl)

Conductivity(Ndlos/cm)A.lkalinity(mg CaC03/L)

Hardness(mg CaCO3/L)TR chlorine(mg/L)Temperature (°C)

_||| _ § � � |

nN MMu.

100% inakierig---- I ,--# {x ,.. | ., SDO (mg/L)Conductivity

(mg CRC0O/L) 3Hardness(mg CBCOVfL)TR chlorine(mg/L)Tempver ture (°C)

Environmental Testing Solutions, Inc. Page 6 of 6


Species: PimephakspromelasClient: TVA I Sequoyah Nuclear Plant - UV-treated

Date: nZn4b4

qI Day I .I - - - -

I4. _ .Analyst I

Concen- ParametertratlonI

CONTROL 81H (S.U.),_ \_._ ., . .,

DO (mg/L)_ _ \_ _, .

Conductivity(pmhos/cm)Alkalinity(mg CaCOIL)

Hardness(me CaCOi1L)_

Temperature (C)- 6. -

OR (S.U.1,_~~4 -_._.-

10.98% DO (mglL)Conductivity I

(pmoscm 1Z-44 IM Z4I I aZ4 I

1o 7- _

Is Z86 @

M v~Temnerature ("C) ZS.A 7ZI.7- 2S.7

-(S.U.) I .16 *22% DO (mgtL)I --. =

Conductivity 7I.O Zw I

- Temperature 7C' X . \ .'l. Z.S- 2..

- - n

7.65 ME Z'7'q1 2_5. % I 1.4 I 'Ld I( 21 .(o z

-

H (sMU.)-- .- -- ._. v*- w

43.9% no (mOL)Conductivity I(plimho s /ci m) I2Temnerature (C)

pH (S.U.) 4.Dd472% DO (mg1L) i

Conductivity I H(eemhostcm) I"3Lemperature (°C) 25. 1 L

_ a0om 1.0 S. 1'7

-4. qq 8,Q8.16 _

. -Z2z 2 ZsI *5 - 6 zZ 28- _

oz I *

= 6.z- 1.4I= 6.4 J. P

L 2.5. zY.4

=8.z Z.7

Z1a55

_ 8Z5 7T-MA.L 4 *14

011 (S.U) -,_ \_._., _ _ . .

100% DO (mglL)_ _ \ _, _ _ .

Conductivity(pmhonMcmAlkalinity(mgCaCHardness -.

(mg CaCO/L) ITRChlorine m i

Temperature (C),

100%intake

pR (S.U.) I 0l5

DO (mV7g) . Z

4.1 W0 Z C.a . cF.cI a. C- I kw-C

k645 Q9.2fz Ws 6.1B. fI .0to :1.0 Be 8z'

I l_ 00o 21i 15

8.4--Conductivity

mhoslcm)Alkalinity(mg CaCO3/L)

Hardness(mg CaCOVL)

TR chlorine ni

Temperature (C)__ ._ , _

I I Il I 'ZS _I Final I Initial

IIIIII

Page 41Pate I of Z -Environmental Testinn Solutions. Inc.

Alkalinity(EPA Method 310)1)

Matrix: Water, MDL -1.0 mg CaCO3/LAnalyst [ 57 ]

Date analyzed _ O1 A I Titrate samples to pH = 4.50 S.U.

Titant no and mdtiplier deetrmiadon: -pH of Normality Normality (AM of H2SO4 pH Factor or Multiplier

Delonized Titrant check Begin End Total (5 ml Na 3CO3 x O .O5)E - (Ni 50000)x 100 ml sample-water reference standard ml ml ml -0251E -Nx 500

* 4.5 S.U. number number (E) (acceptable range - 0.013 -0.022)

5b3- Itojll - 1 IMIC ° 5 I.1 IZ Ue % SaZz -Vo ~ ~ 0.1 a 4 5

Lzborato control standard: _

Referene True value Sample AIkalinfly(MV) %RS-MVITVx 10stard number (TV) volume Begin End Total Multiplier (mg CCO3,/L) (aceptabl ringe

(mg CaCOVL) (ml) ml ml ml -S0to 11%)

W_ 615 s 6-5 100 100 u o _L c.o too toy

Duplicate recision:Sample Alkalinity %RPD

Sample Sample ED volume Begin End Total Multiplier (mg CnCOjL) H(S -D) I(S+DY21) x 100

number (ml) ml ml mil (acceptable range - k 1 %Y.)

Duplicate 4 _________oz__________l- __

Matrix spke recov e.,Reference Spike value Sample Spike alkalinity (A)

standard number (SV) volume Begin End Total Multiplier (mg CaCOCWL)(mg CsCO)JL) (nl) ml |ml ml |_|_I

U S 6 5 1 1__ _ _ _ .0 MM_ _ _ _ _ __._ _ _ 1ZI

- | Sanple alkalinity (B) |Measured spike value (MV) % R MV I SV x 100

(mg CeCO,/L) MV-A-B (aceptble rngeI (mg CsCOj/L) 75 to 125%)

Z50Sample measurements:-

* Sample volume Begin End Total AlkalinitySample number Sample ID (Ull) ml ml ml Multiplier a(mg CCO0IL)

&-2A-0L 12ALA i T o to (4.1 AO.o T ____ to I

- LL O te0 &' i 55

MRS 16zaA *' _1_

I?.1 Ja-1 1To (I

VID-4i)D>|fi 3 ll| '|1 -7|1't| 1 | l

I14 1__1I M£~~~ 2at reviewed:- _ _ _

Reviewed by: I| 1z Dat e dDate reviewed: ro-l-e-04 71.

I

I -I ,iI'

Page 4zPaVP 7 of 2-Environmental Testing Solutions. Inc.

Alkalinity(EPA Method 310.1).

Matrix: Water, MDL - 1.0 mg CaCO3/LAnalyst CP

Date analyzed [^ 1 ji,64 Titrate samples to pH = 4.50 S.U.

ZXfrant ormsalityand uldtplier deferatination:pH Nz,- ormallty _ -Normality (A) of l2SO41 pH Factor or Multiplier

Deionized Tltrnat cla,-en End Total (5 ml Na2COj x 0.05)yE (I [H S0000)/ 100 ml samplewater reference standard ml -- Mnl - 0.2S/E - Nx S004.5 SMU number number 0t i~ .e018 - 0.022)

Laboratory control standard:Reference True value Sample Alkainity(MV) %RS-MYi1Vx 10

ctandard number t) volume Begin End Total Multiplier (mg CaCOVtL) (acceptable range(mg CSCO,/L) mX1) nl ml ml 090 to 10%)

X35S IK 100 100 10.2 Zo i .o,

DuPlicate sam Il precision:Sample _ Alkalinity %RPD =

Sample Sample WD volume Begin End Total Multiplier (m&g CCO,/L) t(S -D) Ii(S+D)12) 1x100number (Ml) ml ml ml (acceptable range IO 10%)

Li bO I f too zi [r n q 0Om s Zq cDuplicatc D

___Reference Spike value Sample | l t (A)

standard number| (SV) | volume |Bein End Total Multpljer (mgCRCWL)(mg CaCOAL) ..(ml) m l a niml

Sample kalinity (B) Measured spike value (MV) lye R - MV/ SV s 100l I CaCOWL) I MV m A * B (acceptable rangeI (mg CaCOzIL) i75 to 125%)

Sample measurements: _____

| Sample volume Begin End Total AlkalinitySimple number Sample ID (ml) ml ml ml Multiplier (mg CaCO>tL)

IOO0OD+1 SD5 5t 1~ -3 I C L1131t 1D 1 tI001W.oI01 LA , Lft, | 13 | 141 |5A |9 - | SB

D o 'ti.D 1 C I I .Q l154to 10 .5 1_10___

q-io&.o soJ-0 ~A2.o ____ ________ID

IonooW .CNQ £ I ISB 3061 q 1 1 1 rz,I

Reviewed by: 1 Dae rDate reviewed: I 01-1-C-OHI

.

kPage I of 2.Environmental Testinl Solutions. Inc.

IIIIIIII

A

C.. ,. -

I.'-S..

I

Total Hardness(EPA Method 130.2)

Matrix: Water, MDL - 1.0 mg CaCO3/LAnalyst [ A*AA|

Date analyzed [ i1 l2 II

rtrant nor alit and intl dier determination:

Titrant Normality check Begin End Totl Normality (M) of EDTA pH Factor or Multiplierreference standard ml ml ml - 0.21E - (N SOO0)1 S0 ml samplenumber number _(E) (acceptable rnage -O Olt -0 .022) -Nx 1000

Itab I 15$ IS 41 Z .O 0l 0.0

Laboratory control standard:_

Reference True value Sample Hardness (MV) % RS - MV / TV x 100standard number (TV) volume Begin End Total Multiplier (Mg CaCOIL) (acceptable range

(mg CaCOIL) () ml ml ml *90 to 110%)

M5- llot40 so C>- ^ sw 112i'

Duplicate son Pk precision:Sample Hardness %RPD -

Sample Sample ID volume Begin End Total Multiplier (mg CaCOIL) (S - D) I[(S+DY2JI 1 100number (mI) ml nl ml (acceptable range 1* 0%)

Co4, " A 11,It. tSAq 4,1 7-M s ::t-£,.,Duplicaw 6 A L. _ D 84

Matrix spike recovery:Reference I Spike value Sample Spike hardness (A)

standard number (SV) volume Begin | End | Total Multiplier (mg CaCO31L)(mg CaCO,L) ml) ml ml ml

1"SS 000 40 to', l54 2zo l t0. Z04 % 24

| Sample hardness (B) Measured spike value (MV) % R - MV ISV z 100(mg CaCO31L) | MV - A -B (acceptable range

| (mg CaCO3/L) | -75 to 12S%)

1 _34 _ I+ 1:021,Sampl measurements:

Sample volume Begin End Total HardnessSample number Sample ID | (l) ml ml ml Multiplier (mg CaCOAL)

I Blank 5sNO (should be - 0 mg CaCO)| On %-% |O b || hD

W A -L (01AS th- _A__ -ZQ ( &.1 AL19* lOts 12.0 -2 _ _ _ 1 __. . 1

L Im o A - -?. I is 4t I 84

S56 - 6 ?. - 1C 4 I 1 _ 84

1: I b L.. S Q -T_ T- _ _ -1

tD-1e Io 1 1.5 I- 14ht Ptt i b 6 .0 _ 23 _ _ a t A h

>l ~ .a I | _ _ 'I Ct. 1e414 ; |

Note: If >5ml ortitrant is used sample must be diluted.by:

Reviewed I E3,_ Z Datcreviewedj ; * _ I_

Environmental Testing Solutions, Inc. Page Z. of &

Total Hardness(EPA Method 130.2)

Matrix: Water, MDL - 1.0 mg CaCO3/LAnalyst [I amZ--

Date analyzed I on 1 ii 4

Titrantnormalfi ann i ermfnadon:Tltrant Normality check Begin En Normality (N of EDTA pH Factor or Multil

reference standard ml ml l - (Kr 50000)SO0 ml s:number (E) I cephtable rngee i -Ni 1000

Laboratory control standard:.Reference True value Sample Hardness (MV) % RS - MV/ 1

shndard number cm volume Begin End Total Multiplier (mg CaC0u/L) (acceptable m(mg CAC03/L (ml) mnl ml ml-S0t X5

______________________40 50 ____ ___ ___ ____ ___ __

Duplicate san PIc precisbon:Sample J Hardness %RPD

Sample Sample D) volume Begin End Total Multiplier (mg CcC0i/L) t(S-*D) /l(S+Dy/2jjnumber (ml) ml ml ml (acceptable range i

iA4oS L^ W Q 0 n4 ej 2 4 0;-! -. CIODuplicate 4 D264 [46 - 1 D 0

arix secove: _ _ _ _

Reference Spike value Sample 1 Spike hardness (A)standard number (SV) volume BegIn End Total Multiplier (ji CSCo 3IL)

(mg CaCC,/L) (ml)| m! ml ImlII

Sample hardness (B) Measured spike value (MV) %R - MV ISV x 100(mg CsC0/LI | MV-A - B (acceptable range

(mrg CaCOL) 75 to 125%)

Sapk Snasmprelents _l! -- -

Sample volume Begin End Total HardnessS le number Sample ID (ml) ml ml ml Multiplier (me CaCOIl

Blarnk - - -|____________ (should be 0mg CaCO3/L) _ _ _ _ _ _ - - - _ _ _ _ _

z .

NO |I0fbD | zQ, I 3 5I 5 MIJzIII Date°[

be - oe~tb Tm Cd CU | 3.|7o|i ; |

. .-.Note- It >15moftitrant is used, sample must be diluted. Reviewed | x| Datereviewed

by: 0

1100C 10%)

.Ii

Environmental Testing Solutions. Inc. *1.

Page J :.Page I of Z

Total Residual Chlorine(EPA Method 330.5)

Matrix: Water, MDL - 0.10 mg/LMeter: Accumet Model AR2S pH/Ion Meter

Analyst LPADate analyzed [ .I I1L]

lodidereagent: !11lAcidragent: IIN 1u]

Coibradon:

I Reference- itandard number l- -AKAle: For samples with a residual chlorine of= 1.0 mga, die calibration range must be austed t bracket the chlorine levels of the samples.

Laborarog control standard.Referencerstandard True valeTV | Measured value (MV) %RS-MVITVzl00

number (mg/L) (mg/L) (acceptable rne- 90 to 110YeI "Ss 1q7 0.5 __ 05.'

Dupicate sampieprecislon: _ _Sampk Sample ID Sample characteristics Residual chlorine YRPD - ItS - D) [(S+D)1211 z 100number I _ _ _ _ _ (acceptable range - A 10%)

{YD401 .04 Asku...... ls YtGe cv, Oaz 4;Jor pS k.a......... 3tI Duplicatek D.0 15_

Sampe Measuurements:Sample Sample ID Sample characteristics Residual chlorinenbmber__

1 Blank (should be" - 0.10 mg/L) <.! zO Iobm vQA - 74- M o 44

._ _ . __ I 6.s 0

. O4>1tiaO I 0 k __!__iCoCA 2+ 1. Q oi' <-e ____ (-__R _ _ _ _ _ ____ _ <_ ._0_ St_

Now All saples wi alyzed in excess ofEPA tecommnd lolding tme (t5 nminutes) unless atherwise noted.

0 aep?-JOS~I-of

Reviewed byDate reviewed

_ _Page Z of Z.

Analyst Wanalyzed 0-1-6-7-0E�


Matrix: Water, MDL -010 Img/LMeter: Accumet Model AR25 pH/Ion Meter

Iodide reagent [ 14 e t 77 1Acid reagent [ I , I ,>

.the 1.0g mgeL - - I

3'L, the calibaion inge must be adjusted Z bracket die chlorine levels ofthe umles.

samlyle Dlem~uremen&s:

Sample Sample ID Sample characteristics Residual chlorinenumber (mdL)

stt,^,gt Blank (should be < 0.10mglL) m j

o h.t~v tc5 oo cdte n AeV cLs co-oozP'9

Dolmbz 5bs lo t * ~ :~ &-s .,,PJ I46fb> {)0 oltibD~oirIWA& pW .b& 4z l <O.O0tVL4.

Note: AlU saleswcre alyzed In excessofEPA recommendedholding tme(15 minutes)unless othevwisw noted.

Laboratory control standard:Refereuce standard True value (TV) Measured value NV) % RS - MV/ TV x 100

number (mgL) I (mgL) (acceptable range - 90ob 1101%)

loss I -- 0.50 0.4b q7. /Reviewed by I. ZZ]

Date teviewed I 7D 1


Page I16Page I of


Matrix: Water, MDL - 0.10 mglLMeter. Accumet Model AR2S pH/Ion Meter

Iodide reagent: [jjiqI. ]Acidreagent: | 1FjV17 _ 1

Analyst E ftoDate analyzed la . o9 . 0i

Calibration:I r . -, - 1 i0.10 I 1.00 m I

I Reference standard number _ 11j5S ;q7 | I j5s III I

NAote: Forsamples with a residual chlorine of > 1.0 gL, de calibrtion tange ust be adjusted to backet the chlorine lvels of the samplc

Laboraory control standard:Reference standard True value (TC) Measured value (MV) %RS - MV ITV z 100

number . (OgWL) (mg L) | (acceptable range - 90 to 110%)

It 0.50 0. 51 i 103. o%

Drlicate sample prcislon:

Sample Sample ID Sample characteristics Residual chlorine %RPD - ((S - D) /[(S+D)j211 x 100number 4 L) (acceptable range - a 10%)

2i N 0 1Asy4. 1J otol e( Ci S_ I Duplicate D .NO_

Sample measurements:Sample Sample 11 Sample characteristics Residual chlorinenumber (In__L)

- Blank (shouldbe-cQ10m0 L) < 0o.oo9l

401OG. Oz 50Q? 10 do Col06r, ia *.Do 7 ;oC5 g.o 6

Note.: AU! samples w.r analyzed in excess of EPA itcommended holding tisne (U5 ainates) unless ofthwise noted.

Laboratory control standard:-I Reference standard True value CMV Measured value Om) %RS-MV/TVx1OO

number I(U19L) (MgQL (acceptable range - 90 to 110%)

I .o 0.50 qq 5 ,

Reviewed by I KwDate reviewed rfl3njJ

If

Environmental Testing Solutions, Inc. 583

Page I1Page I of I


Matrix: Water, MDL - 0.10 mg/LMeter Accumet Model AR2S pH/Ion Meter

Iodide reagent: [l) JRlISAcid reagent: | 1 _lO

I

Analyst _ _

Date analyzed (-O. jjl C

I,

I �'�4.

1j.I

I�

� *?-I �I -� �

I *�*.;'I

I .,4*'t' 4'-.

��;

Calibration:I -': :-' ' !-; *. 1 ; ss'- "~wgL t l~g

Reference standard number I 0.1sslq0 + I I.0 bGAI INote: For sunples with a residual chlorine of > 1.0 mg/L the calibration nmpge must be rusted to bracket the chlorine kvels of the swmples.

Laboratory controlstandard:Reference standard True value (TV) Measured value (M) % RS- MV/TIV x100number (mgwL) (uL) _ (acceptable range - 90 to 110%)

_ 0.50 n s 1. 11

Duii cte sample recilon:Sample Sample ID Sample eharacteristlcs Residual chlorine %RPD - ((S - D) II(S+D)12l) x 100number (mL) (acceptable range -J 10%)

~ L Q I . ~ Z ~ b A ~ r ~ ~ ~J a . I S £ 0 . C ~ 1 O _ _ _ _ _ _ _ _ _

Duplicate ` _D 4____ _ jSampl mJeasurements:Sample Sample ID Sample characteristics Residual chlorinenumber (mgVL)

0 Blank (should be - < 0.10 mglL) 02 Omi I:o % -io .o ~ a-~~r . 0 .0 0 524

O ~t4 0t r f b M ' a # LO A i c . £ O . 0 3bLIO- io. 03 rrC6V Fx- n L) W,>*zu pjsx1i)40 o C IV~~ '?NeA SS f loC. 010 Cuwa i0,005V1o'Jo 0'7.OZ- P oli o no c.14&v. CUOLO 0 53

DqO?10 p 1 IV A r , r ocHwy- ________

0qon ac.o r _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ j j j q j jL 0 .Oc qc9

Note: AUl samples were analyzed in ecess ofEPA recomended hg( m s

Laboratory control standard:.Reference standard True value (TV) Measured value (MV) %RSMVITVZ 100

number (mgtL) (mgL) (acceptable range - 90 to 110%)_ _ s JS5 0.50 O. 9O I 7.

Reviewed by I ff :1Date reviewed I CV4Q-11 .)

Sequoyah Nuclear Plant BiomonitoringJuly 7 - 14, 2004

Appendix DReference Toxicant Test and

Control Chart


Potassium Chloride Chronic Reference Toxicant Control Chartfor Piinephales promelas

using Moderately Hard Synthetic Water

1.0

0.9

0.8

I I I I I I I I I I I I I I I I I TI I

State and USEPA Mandated Control LimitsLimits Set According to ± 2 Standard Deviations

0.7

0.6

9iq

14,19I 0I.

ti.Po

I I I I I I I I I I I I I I I I I I I I

0.5

0.4 F,t.1

- - - - - - - - - - - - - - - - - -1.0

0.9

0.8

0.7

0.6

0.5

0.4

USEPA Warning and Control LimitsLimits Set According to I e and 2? Percentile CVs

.................. I........................................................................................................................... ......

..................................................................................................................................

11, %.,- ;NO 001 01

Test date

0 7-day IC7,s = 25% inhibition concentration. An estimation of the concentration of potassium chloridethat would cause a 25% reduction in Pimephates growth for the test population.

- - Central Tendency (mean IC.)Warn1ngLWts(meanIC25-+SA.j0)

.......... Control Units (mean IC2, ± SAM or 2 Standard Deviations)


Potassium Chloride Chronic Reference Toxicant Control Chartfor Pimephelexpremde

adig Moderately Hard Synthetic Water

Sht41JdUSA 1sb..'t" l 1bfwtM WIJ A UlBPATut _"dMI Twt ask 7I-d"C, CT S CaUrd Lbt 9a WIu limb SAN Cst l Unit SA Won" lmts 83 A1 C I41bnts CV

(fL KCO (IL :CO CT-20 S CT+2- CT-O CT Cr+SA CTS-AS CTr+JiA,, CT-SAM Cr+B, CT-8As CrT+ SAM

1 11-11403 0.532 124903 0.51 035 0.03 0.49 0.62 0.07 0.48 0.62 0.12 044 0.67 0.21 0.34 0.76 25 030 0.Q0 0.063 01-0604 0.66 0.9 0.07 0.45 072 0.07 0.2 0.66 0.2 0.6 .71 0.22 036 0.81 026 0.32 0.85 0.114 01-304 0.64 0.60 0O. .48 Q72 0.07 03 0.67 0.13 .47 073 023 0.37 0.33 0.27 0.33 S.7 0.10S 01-20404 0.3 Q62 0.06 0.49 0.74 007 O.S4 0.69 .13 O9 075 0.23 0.33 03S 2t 0.34 .39 .106 02Q0304 0.66 0.62 0.06 051 0.74 0.07 035 0.70 0.13 049 0.75 0.24 039 0.36 0.2 0.34 0.90 0.097 03-0204 066 0.63 006 052 0.74 0.O 0.5 0.70 .13 0.0 Q76 0.24 0.39 0.7 0.28 m35 Qn9 0.098 03-1644 0.64 0.63 0.05 0.53 0.3 0.03 05 0.70 0.13 0.5 0.76 0.24 0.39 0.37 023 0.3 0.91 0.039 04K-064 0.60 .63 0.05 O 0.72 0.08 0.5 0.70 0.13 0.49 0.76 0.24 0.39 0.36 .2 0.34 0.91 0.0810 04-13-0 0.64 063 0.5 O 0.72 0.0 0.55 Q0 0.13 0.50 0.76 0.24 0.39 0.37 0.23 0.3S 0.91 0.0711 04-20.04 Q63 0.63 0.04 0.54 0.72 0.0Q 0.55 0.70 013 0.50 0.76 0.24 0.39 0.31 0.28 03S 0.91 0.0712 05-06-04 0.63 0.63 0.04 0S4 071 0.0" 0.5 0. 0.13 0.50 0.76 .24 039 0.87 23 0.35 0.91 0.0713 05-114 0."64 0.63 004 0.55 J0.1 O0. 0.55 0.70 0.13 0.50 0.76 0.24 039 0.37 0m 0.35 0.91 0614 05-13.04 0.56 0.62 0.04 0.54 0.Q1 0.07 0.QS Q0 0.13 049 0.76 .24 0.39 036 0.23 034 0.91 0.0715 05-1344 0.58 0.62 0.04 0.54 0.t1 0.07 0.S5 0.70 0.13 0.49 0.75 0.24 0.39 6 0.23 0.34 0.90 0.0716 06044 0.61 0.62 0.04 04 0.70 0.07 0.5S 0.70 0.13 0.49 075 0.24 0.39 0.86 0.28 0.34 0.90 0.0717 06-2244 0.57 0.62 0.04 0.3 070 0.07 0.54 69 0.13 0.49 075 0.23 0.38 0.5 .21 034 .90 .0713 07-07-04 .54 0.61 0.04 0.52 0.W 0.07 OS4 0.Q6 0.13 043 0.74 0m 0.38 0.35 023 0Q34 .39 0.0719 07-144 0.55 0.6 0.05 0.52 0.70 0.07 0.54 68 .13 0.43 0.74 0.23 03t QU4 027 0.34 0.33 00820 07-2014 052 0.61 0.S 0.51 Q70 0.07 .53 0.3 0.13 0.43 0.73 0.23 0.33 0.54 0.27 0.33 03 0.03

NOW 7-4 IC,.- 7d 251 4-a emoh Autsthm .t . tat 25% m l hPie_ g fitbe ttpCT- 1kmwdnlba7J:..O.

8 - 81nrdd doibt fffie,. vnhe.

1.tcy wu ng wbW~fft04dUhIdwitdw diitn d w tefthe uleqa4tOdi 10.mXd2Muein C..Th.. pa- sihatfi.. iemmr mdadhgusueinedeU S !PAf@edi t..tineo l d mde4.

SA8d- 3ffaVGbft9 srpbg 0Ipat&CV. (01-an)

pM 1t~dwdd~pbn O"pOMigfo fie a 2 CV. (8M-0.21)UPAC.tudWa Ina ft

SA ,,- t.dwdn aetI ew-potglew 7pA , CV.(8A -033)

SM !tininfld & QW"en PMudag9b 0epennlkCV'. (NA.O-OAS)CY - roem utrM. at fwc, Vakw.

tfA. 0 tlMdfmdAiw.Ekk tletlW ndf gbd h t l3tpbxbPA4033&-MM UN fIti WoaA ~ ahI OI

hindrk dja 07-2


Precision of Endpoint Measurements

Potassium Chloride Chronic Reference Toxicant Datafor Pimephalespromdlas


Teat aumr Test dateControl Control MeanSurviv GoWth Cr CV cr IMS PMSD CT

(X)(MZ~fM) for Contrul Ckow&h

(invurve) M%) Orow&hCV (%) (%6) for PMSD (%)

1234567891011121314151617181920

11-11-0312-09-0301-06-0401-13-0401-20-0402-03-0403-02-0403-16-0404-06-0404-13-0404-20-0405-06-0405-11-040548-0405-18-0406-08-0406-22-0407-07-0407-13-0407-20-04

10010097.510010010097.510010010010010097.5100100100100100100100

0.9S30.7440.8160.8070.7700.847O.S660.6860.7080.7800.7840.8320.6SS0.7180.681O.8S90.7000.6100.8050.727

0.8480.8380.8300.8180.8230.7860.7740.7660.7680.7690.7740.76S0.7620.7560.7630.7590.7510.7540.752

11.62.24.63.316.55.24.87.312.09.85.55.513.14.49.31.315.111.110.215.6

6.96.15.47.67.26.96.97.57.77.5.7.37.87.57.77.37.77.98.08A

0.200.180.130.100.130.110.080.080.140.110.080.100.110.080.120.080.120.080.120.17

21.324.215.512.517.013.014.812219.313.910.212217.110.517.89.616.813.615.423.4

22.720.318.418.117.216.916.316.616.415.815.515.615.31S.415.11S.215.115.115.5

Note: CV coefficeat of "nration for control grwfdL.

On rage, Me CV for control growlh i 8IA% in Environmental Te ing Solbtions, Inc. PFm4hilks chronic toxicity test.

Lower cV bound determined by USEPA (l0e percenile) - 3.5%A

Uppa CV bound determined by USEPA (90' peeanile) - 20%

MSD - Minimun Sigafficant DiffercePFMSD Percent Minimum Signiant Difference

PMSD is a measue of test pciso The PMSD Is the ninim percent difference betwen the control and treatment dat can bedeclared statstically significant *na whole effluent xicw test On avrage, a signifcant differcnce occun for En-miental Testing

Solutions, Inc. chronic toxicity tess when a toxicant reduces PFwhalar growthby 1S.5% firom fg controL

/ Lower PMSD bound datennined by USEPA (10t percenfie) - 9.4%.

Upper PMSD bound detemined by USEPA (90' p benle) - 35%

CT Central Tendancy (me Control Growth, CV, or PMSD)

The lower and upper bounds weiu calculated by dle USEPA wing 205 eIc conducted fro 19 laboetrics for Phnephals growfliin chronic reference txicant tests.

USEPA. 2000. Understandin andAcunitg for Meod Vably inWhol Effu=tToxicityAppcat s Undr Ie Naonal PollutatDisage Blitinn Progam

EPA-433-R4O003. US E hvimnnentl Protection Agency, Cincmiat, OR

Orgaiztrm otb~zhedfrom Aqua&c BioSyst=Am Inc.0-00 07-20-04

Environmental Testing Solutions, Inc.Potassium Chloride Chronic Reference Toxicant Control Chart

for Pimephales promelasusing Moderately Hard Synthetic Water

1.0

0.9

0.8,ON... 4

U

X

9inC4

U

PC

,it

0.7

0.6

0.5

0.4

. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

. . . . . . . . . . . . . . . .. .. _...-. _............................................................................................................... _... _... _... _... _..._.ow.. * * _* _..

.. .. ... ................................................................................................. .

- ------- - -_ a

I , , , , , , , ,

0.3

0.2

Test date

7-day IC25= 25% inhibition concentration. An estimation of the concentration of potassium chloridethat would cause a 25% reduction in Pimephales growth for the test population.

- - Central Tendency (mean IC2.)

Laboratory Warning Limits (mean IC25 i S.,I0, S, = 0.12)

.Laboratory Control Limits (mean IC5 SAM, SAM= 0.21)_ _ USEPA Warning Limits (mean IC2. + S, 7 5 , SA.7S = 0.38)

- USEPA Control Limits (mean IC2. S% , S% = 0.45)


Pimephalespromelas Control Growth and Coefficient of Variation

in Potassium Chloride Chronic Reference Toxicant Tests

1.50

1.25

. . . . . . . . . . . . . . . . . . . .II III III I I I I I$ I I I I I IT I.

I

TF

1-1

1.00

0.75

0.50

. . .........................................................................................................

.................. ................ ............................................

0.25 _.._i._.m A_.._t c Criteri (0_. 2 _ per .. _..ng _. . _. ._ ... _.Minimum Acceptance Criteria (0.25 mg per sunviving larvae)

I I I I I I I I I I I II I I I I I I

I I I I I I I I I I I I I I I I I I I I

m0 .

ar 3O 9

g

40 _.................._._._._._._._._._._._._._._._._._.North Carolina Acceptance Limit (< 40.0%)

Kentucky Acceptance Limit (< 30.0%)

20

e0

Test date

a Control Growfth or Coefficient of Variation (CV)

- - Central Tendency (mean Control Growth or CV)

. .. Control Limits (mean Control Growth or CV M 2 Standard Deviations)

Environmental Testing Solutions, Inc.Precision of Endpoint Measurements

Potassium Chloride Chronic Reference Toxicant Control Chartfor Pimephales promelas


40

30 1

00I

a

1 1 1 I I I I I I I I I I I I I I_ I

.................................................................................................................................................................................................

............................................................................................................................................................ ........................

20

10

0&-L -r

Test date-e- PMSD - percent minimum significant difference. PMSD is the minimum significant difference

between the oontrol and treatment that can be declared statistically significant- - Central Tendency (mean PMSD)......... .Lower and Upper PM SD Bounds

Lower PMSD Bound (1 0̂ percentile) - 9A'4, Upper PMSD Bound (90e percentile) 35%(LAwer and upper PMSD bounds were determined by USEPA for the method and endpoint)

Environmental Testine Solutlons, Inc. Page I of 5Environmental Testln2 Solutions. Inc. Page lof 5

Potassium Chloride Chronic Reference Toxicant Test(EPA-821-R-02-013 Method 1000.0)

Species: Piinephaekspromelas

PpKCICR Test Number: AS

Diludon preparation Informaon: Comments:KCICHMnumber: CIAM l'42Stock preparaion 50 g KC/L Dissolve 50 KCIa in l-L

Deionized water _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

Dihtion prep (mt/L) 300 450 600 750 900

Stockvolume (mL) 6 9 12 15 is

Diluentvolu wniL) 994 991 983 985 982

Total volu=e(ML) 1000 1000 1000 1000 1000

Test organism Infornaton: Test Information:Organism ge: 2L.Sm 2a t 0 : Randomizing tplate: 6Zj

Dateandtimesorganisns 01 ot-Atq -MW WD I i Incubatornumberandwere born between: Mao mIO MOe r shelf location:Organism source: ASS B 0- c -ol4 Anemia lot number: A I OSSTransfer bowl informationm pH - SU Temperature - C Total drying time: s

_.__q 24.5 Date/ TIme in: 01rL- V 14N5

Average transfer volume: Date / Time out el=&_ ,_ _ Oven perature: '

Dailyfeeding and renewal Information:

Day Date Morning Afternoon Test InItIatIon, Control water Analystfeeding feeding time renewal, or batch used

time termination time H4lZo 01c204 *M-q Ioc 1-6 l CILI

1 l -Zl- mQ0os ISt; 19. 01nX~ - 6

2 u2ivoq t>1S I'S 0 t1tsb= 0o1- Iq -t1

3 23 O&S iSil o V4 0-cS.4 01-2 o&l t0516 VS2.2 1t6-1 01-11-C!tA

01- z$ o t'Sco J0L Z 15C , '6 _.

al21-V * S% r s ____ ____ __ __ _

Control Information: Acceptan e Summcry of test endpoints:% Mortalitpr. OI) s 20% 7-day LCsq 331f.lAverage weight per initial larvae: 1 e.12b NOEC -q50Average weight per surviving larve: 0.1 2 0.25 .me LOEC 6 00

ClVIC2s I 5z.

I Environmental Testing Solutions, Inc.


I:.

Page 2 of S

PpKCICR Test Number. q

Survlrd and Growth Data

I111'

I

.1IIiIIII

Day CONTROL 300 w KCUL - 450 m KCUL -

. A B C D E F I G H I J K L

0 to JO 1_ t0 1o 10 10 10 10 1O 10 to

I 10 to IQ to /0 (0 Io /0 /6 /0 /6 /0

/2 /Q jo Io Io lo 5o S1o lo /0 D A

3 lo / _ Ito 1 _ I 1 to to l /

4 JO io/ 10 10 do 1O6 It J O

S 1D / a jo I 10 1 1t/0 1o

6 J /0 /0 10 10 lo 10 lo /0 /0 JO /0_ _ _ _ /0 /0 10 10 / 16 0 o to0 t /0 /60.

7 ,0 8t 0 _6 to _ -( 1_ _ It o o t 1 o'A - Pan weight (_g)

TAAY el isx 6*. r oo s %6AX, 401% "Jq 14 16.0f w*w 6%B-Pan + Lame weght(MO ZZIS4 21.5 .2Z 2t.65 11.61 tZ.80 Ul.o 2s-I 11.15 ZZ.11 Zk28 ZoK

C-Larvaeweigbt(mg) @-A-B 'lb

Wedght per Intal number of _ _ A _ _e _ l _ _larvaefmg) ~~V ~ ~ .~- Cl InIialm umber eflarvae Ir t O f' O. OtAverage weight per initalt _sumberof larvae (mg) . .17 VI o.516

_ .- _ ,_ . _. a .

Calcutations and Atar rvlewed. 6 \

_

Comments:

II ..i I

I _

IIII

1.

IIIIIII

l

;nvironmental Testing Solutions, Inc. Page 3 of 5

Species: Pimephalespromdas PpKCICR Test Number: Ao

Comments:

I.

I - . -


Chroni Whle Adft To*It Tet (EPA4214t041X Metod 10dSpey:rn Pbepwespvfmdw

Q112ifty COtrlMVel'fICatleR Of Dta EnRy, Calculationst, and StaIdtial Anal"e

TatmOu_ DpKofseetwnftwEL

Tat ak = Ay l-27, 1 Jtfwlw#dbp=%-.01 I0 - - - z ~" bw l#-i_ - -

- ^. S- - - - a - -

to 0 1489 _ 22Ss 7.96 _ .794 0.7_60. 80 10 14.93 21.38 63 0.63 0726 15.6 0.638 IOU s7i SAS NM *"snbatC 8 t IS."0 2.2 62 0.622 0.422

-D 10 10 1i.s6 2363 80.49 o _0.549

1 80 10 15.13 21.87 6.74 0.674 0674to i s3.21 22.80 759 0.7t7 00. e759 ^c14 _ 21-7 634 1 0.634 d634 In I_ 14n 2297 7." 3 0.74 079

I. * J 104.73 19.95 527 0 07 4.274" H 80 o 4.4 227 799 0-504 0575 22_ __ 0.799 __ 2AZN

L to 0 84.68 It I 5.02 0 -. 27 0.500-t3.04 21.24 63. 0.620 e 620

OV l ow 14.70 20.13 5.43 ea1 s.l - 0571 - 1.IO 4 X t 2#o- l * 14.59 22.1 76 0.69 0.49"IF t . 9 14.32 _ _n0.442 0399

802 14.26 83.32 8.26 0.630 0.1267I t0 4 14.76 16.11 8 13 S 1-- St 25 1 0.33 nJU g6 31 7T

-S= t0 4 13.32 1735 223 0.3 o2310 5 41A6 1624 1.39 0.460 O_ .3_ _ _

U 10 o o.oo 0.03 0.000 0.000t V _ L. 2 .47 I4." o1 807 0.485 0.650 20.6 0.097 iRs IIn" "A

W o e. 000 000 0o00 o.00 0.0330x 1 814.62 8527 065 _ 0.650 0.065

- - - -- -SD -_ D

r111% 2ISA 9MS- Po Mepwmi6D88hsw.ss

Lo198bw4uwhbyWA(00,pw_)h 9.4%(JNwSDft4S 4 0,W elile)33%

Thalwumiqipaeid vwa-IduulbydeUWAtwft205 4.nemluui Wt 89 N~uI kelrra.qlid pw6,h imm .fowukwconwiL,

use& 2800.Ux.044.4UA~mlMU- *r~Oud &Vubhfd8tyW hlE~uMtTediyA99 1.mUmbvtmfaif P. Maut.idm. thedookah~,. FPA833*.C0402. U515 U.wmid wonhApqC.duwu4OK

D t A Okmiw.A'w*DbwMm00 eat0-0

Environmental Testing Solutions, Inc.Statistical Analyses

LArval Fish Growth end Survival Test-7 Da Survival

Start Dat: 7/20/2004 Test D. PpKCICR SanO1lD): REF-Ref Twicont

End Dat: 7=/20 Iab 1D. ETS4nvir. Testing Sol. Snple Type: DMR-Dicgo Monitorng ReportSanpe Date ProfltE CHRONE43PA-821-R-02-013 Test Speciee PP-Pinepbales prwnelasConmynb:

Caue-iL 1 2 3 4DCol 1.0000 1.0000 1.0000, 1.0000

300 1.0000 1.0000 1.0000 1.0000450 1.0000 1.0000 1.0000 1.0000600 1.0000 0.9000 0.8000 0.9000750 0.M00 0.4000 0.4000 0.3000900 0.0000 0.2000 0.0000 0.1000

Transform: Aresn Square Reot Rnk i-Tailed Number Total

Cone"eglIL Mean N-Mean Mean Min Max CV% N Sunm CrfitCal Resp Number

D.Cono l 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 0 40300 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 18.00 10.00 0 40

450 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 18.00 10.00 0 40600 0.9000 0.9000 1.2543 1.1071 1.4120 9.935 4 12.00 10.00 4 40

0750 0.3250 0.3250 0.6032 0.4636 0.6847 17.472 4 10.00 10.00 27 40*900 0.0750 0.0750 0.2757 0.1588 0.4636 53.294 4 10.00 10.00 37 40

Auxiliry Tests Statistic Citical Skew KurtShaphro-Wift Ted idaod"s non-nonnal distnbution (p <- 0.01) 0.84628701 0.884 0.2491888 1.10337554

EBqaity of vtrin cannot be confirmedHypotheds Test (1-tfl, 0.05) NORC L4DZC ChV TUSteels Many-One Rank Tes 600 750 670.820393Trmtentfs vs D4C3otrol

Organisms obtafnedfiom Aquaic BioSystems, Inc. 07-20-04


C"Am aq C~promefew Vd. St 9% MWMUelt P.,.w Mu Mr" 11w-

1-5.84661 1.12162 152952 2.92-402mo8 6.32972 52.69 -2l.M"424

0 0.72310589 7.814727 087 133966 0.0630331 3

Pu _ blt RngL 95% FIuiwIECOl 2.674 5".717081 U3.04463 554.15391PaMs 3355 6216" 5.17121U 60.64266ECtO 3.718 593.174 542.93189 627.549BCU 3.964 614.6633 568.M336 648 2PCM 4.158 632287 50.0 66860PCMS 4326 647.8582 601589006 67292264PM4 4.747 68647563 65.87 6 717.21549DM0 5.000 714.440M66 63061874 744.57094BC60 5.253 74L200262 71.532092 77494BC75 5.674 787.91892 755.3615 833.036LCMO 5.842 807.265421 772355443 85.644309BCJI 6.036 830A6525 10082 301007BC90 6.282 860491423 816.737752 932130IPC95 6.645 907.0173 8579508 99933615ECM9 7.326 101.39037 92."6377 114L1109

1.0

0.9'

0.8

GL7*

aQ4

03

0.2

Q.1

U.U .. _ . .

low 1loo01 10 100

Do. nVL

D Fit

Org-anf obtaftedfi Aquak BoSt Inc.70 07-20fi04


Larval Fish Growth and Survival Tet-7 May CGowthStatt Dte: 7/20/204 Test H. PpKCICR Sanmple D: PIF-Ref TwdoantEnd Date: 7Z7/204 lab Mt ETS-Env*r. Testing SoL Sbnple Type: DMR-Dchasnrge Mowoitng ReprtSample Date: ProtoaL CHRONCY-EPA-821-R.02-013 Tes Species PPPimephales premela

COM4C/L 1 2 3 4D.Control 0.7960 0.6390 0.6220 0.8490

300 0.6740 0.7590 0.6340 0.7990450 0.5270 0.5040 0.7690. 0.5000600 0.6200 0.5430 0.4950 0.3980750 0.1260 0.1350 02230 0.1380900 0.0000 0.0970 0.0000 0.0650

TransforNm Untransformed 1-Tafled Isoto"nCCoue-yL Mean N-Mean Mean Mh Max CVe N t-Stat Critel MSD Mean N-Mean

D-Control 0.7265 1.0000 0.7265 0.6220 0.8490 15.576 4 0.7265 1.0000

300 0.7165 0.9862 0.7165 0.6340 0.7990 10.576 4 0.135 2.290 0.1699 0.7165 0.9862450 0.5750 0.7915 0.5750 0.5000 0.7690 22.588 4 2.042 2.290 0.1699 0.5750 0.7915

*600 0.5140 0.7075 0.5140 0.3980 0.6200 18.075 4 2.864 2.290 0.1699 0.5140 0.7075

750 0.1555 0.2140 0.1555 0.1260 0.2230 29.124 4 0.1555 0.2140

900 0.0405 0.0557 0.0405 0.0000 0.0970 119.891 4 0.0405 0.0557

AurIary Tests Statiste Crtlical Skew KurtShapifo-ilk-s Test indicates nonal dibntrbution (p > 0.01) 0.9246071 0.844 0.59174542 -0.7397034Bartets Tes indicates equa variance.s p - 0.84) 0.83600086 11.3448668Hypothesh Test (1-hal, 0.05) NORC LORC ChV TU MSfu MSDp MSB MSm F-Prob dfDrknet's Test 450 600 519.615242 0.16991969 0.23388809 0.04431933 0.0110115 0.03399318 3,12

Treatments va DCOntrol

Organ0sms obtafnedfrom Aquatic BloSystems, Inc 07-20-04


1ww MOL SD 93% Ca ) Sbw1M 327.91 074 0.00 495.27 -07679

1010 346641 67.17 67.59 M5.19 439171015 40.2 59.8 1.20 "Ca67 o.O t.0

04433 6664 2%063 695.99 0.34151X:2 524M0N MU34 "5 .42 .0.1493 09

040 6316 23. 516.02 66&01 -17043 0.KlC50 663.01 1332 613.12 692.39 .70 0

0.6

0:0.4,

0.3

.2:

01./

0.00 200 400 600 800 1000

Do. neL

0.8

0.7 I-MR, , | M.. . . . . . .. .. . . . ... . . . . . .. .. . .. .I of 1k ~ ec

Orgnm- -bt Trwdfi- Aq-d-*Btogyan Ilna 07-20-04

Envronmental Testing Solutions, Inc.

. .. 3w

.P . .g * e.Page 4 of S - -1. >:

Species: Pimephakspromdas PpKCICR Test Number: O

Daily Chemistry:, _1

I Day II 0 I 1 I 2 I

Concentratlo. .

Parameter

_~. -CONTROL on (S.U.), z _ , _

DO (mztL)s _

Conductivity(umhos/cm)

- .5

AlkalinIty(mg CaCO3/L)

-7. l 1 7-5 175 7.7Y 0-1|fh

0-14 R1 zsq Z;14

SA9 < S6el ! -4;

25.\ 4^ a S. zi. ZS.0 ?A.(

ftardness(m_ CCOAU

4-Temperature(-0

pH (S.U.) § w st.,DO (mglL) 865 . 7.0 l 7TV

300 mg KCIIL Conductivity(Thos/cm) 7 3 |Temperature 24.s

-0 - - 21

nCH (S.U.) A.o I qz I fa.Io v I 4.7 I i.oq I V.B3 IDO (mg/L)

450 mg KCIIL Conductivity _(mhoscm) JI

6L4 j pk .oI ' 6.q1 7-It I 1.3

~1110

?ws M.'l Zq.16

.

Temperature(Pa

pH (S.U.) 8.l ;.Io 7.f o.1eDO (mgAL) B1,7. .9 ?5

600 mg KCI/L Conductivity 3 MC(nmhos/cm) 1~OTemperature Zq .9. .Z4.!

PH(S.U.) .o 7.7I6 _._ _.78.

DO(mg/L) 8.4 .I6 7,q .0 7.s750 mg KCiJL Conductivity 167O

(wihos/cm) oTemperature 25. 2 . q.. z.i zq.o

'aS- - - -_ 6ci (S.U.) .10o I 7.Y.5 T10.W I 7145 I V-c3 I -4.IY I

F - * 4 - - I? �3��II�UI UDO (mg/L) e. I-. 6 Ifq 0 1 -. , 1 .1 0

900 mg KCIL Conductivity(gmhocm) _

Al

Temperature(0C)

aqoo A1 I15 ~

tS.OW~ zq .O 2'. S *4Zg

Inial it Fi00aInitial l a Initial I al IInitial la

STOCK ' tConductlvlty _I (Anhos/cm.) '

Environmental Testins! Solutions, Inc. Page S of SE-iomna -etn S-uios Inc PaeSo

Species: Pimepholesprometas PpKCICR Test Number: _

3 4 5 6An alyst rcS I 15 fe is |o ?C1 % Om

Concentration P arameter_CONTROL jpH (S.U.) 414t.WX°L18 ~ ~

DO (m2/L) 7 l .5 M '7.Conductivity AZe

Hardness

TemperatureDO Ag/l) IT. 2 z'.I ZSSA 2 6

300 mg KCa/L Conductivity

Temperature Z 2s. \

_ p(S.U.) 8o- T 7.5 . 0 0 1.9

DO VAp. L. , o 1 B .O I 1. 11450 mg KCI/L Conductivity 1 103

Temperature .z,.tit 4.2 'ZA 2. . 2q 250M5 mg KCLCnutrA 13° O O3o - 13

p}I (S.U.) 6g S .? Ro . ,05 I., - a-s.o -1.615600 mg KCI/L DO (mg4L7. 7 M

Conductivity

Temperature Z5. I Z.'z. 2Z. 2 25.0 4.j

PR (S.U.) 0._ 7!7 8.01 -LB 615bs-010 78DO (ngWL) 7. Ir 7. .77Z 6 7 B -,

750 mg KCI/L Conductivity

Tempenture t . 2. Z Z.O 2B 2.1 2(M)pH (S.U.) 74.1 - 8.00 I 1.&h I g.oq I '7.18 1 A-in 11.89 I

*6- - - a - *I*�.-�--=---1I- - - -

DO (mail) 7A 1 *1.0 - I 7.?L LI - 1-- 7 1-8 I900 mg KCU/L Conductivity

(tmhos/cm).i

TemperatureM

203c Mt>660 q&mSt 24.G -2.Z z .z ZS. 1 Zsl-t 2. 0 Z.

7390 1S.o ;900Itlfal Final Intial Final Initial Final -Initial I Final

STOCK Conductivity_ I nffmhslcm)


Sodium Chloride Chronic Reference Toxicant Control Chartfor Ceriodaphnla dubia


1.20

1.15

1.10

1.05

. . . . . . . . . . . . . . . . . . .*I I I I I I I I I I I I I I I I I

State and USEPA Mandated Control Limits.Limits Set According to 4 2 Standard D ev ai s ...............................

.. " l,..... ........................ ... f i

(UV

z

tU

%%00

1.00

0.9S

1.5

1.4

1.3

1.2

1.1

1.0

0.9

0.8

w ........................................................................................

I I . .

, I I I I , I I I I , I I I , r

Laboratory Warning and Control LimitsLimits Set According to eh and 25 Percentile CVs

_.............................................._.._. _.._ .-.--..-. _.._ ._...._. _ ._ .._ .._ .. _.. _.. _.._.....

................................. ............................................................................................. ........., , , , , ,.

Jt o ZS Jt" otSA 0 20 JO 0 ! .0o0T date

Test date

-* 7-day IC, = 25% inhibition concentration. An estimation of the concentration of sodium chloridethat would cause a 25% reduction in Ceriodaphna reproduction for the test population.

- - Central Tendency (mean IC2.)

------ Warning Limits (mean IC25 k SAd1o)

.Control LmItS (mean IC25 4 SAM or 2 Standard Deviations)


Sodium Chloride Chronic Reference Toicant Control Chartfor Ceriedapbrni dabia


Tet wme Test t 7-dy WCm CT(AMM~ btLlqo

8hh1. NMUMAB CSewtsLfni

CT-28 CT+28WaCT n8Lhit T+ M

CT -SOA Cr +SAA* CT - PSA CTr+ 8AMOA" Wm 3 1.%ft

CS -SIM C + SAM,

8" C..1i b

CT - 81.P cr + a8,,CV

2234SS7a91011121314151617is1920

M1234201-0740302440303405030441-0303.0-030644C0307103085403096104031047031144031244-030146440200403Q304044060405-4-0406-0407414

L040.6Q.9L051.03L05L07L03L04L051.02L08LOS1.061.06L071.07L07L06L06

1.00 0.06 0.9 L2 O.OS 0.92LO0 0.04 0.92 LOS 0.0Q 0.92LOI 0.04 O."3 10 0.0 0.93LO1 0.04 0.94 LO9 0.01 0.3L02 0.04 095 L09 0.0 0.4L03 0.04 0.95 .10 0.08 0S4L03 0.04 096 10 0.03 0.94L03 0.03 0.96 L09 0.08 0.951.03 0.03 0.97 L09 0.03 0.951.03 0.03 0.97 L09 0.08 0.935L03 0.03 0.97 .10 0.0 0.9351.04 0.03 0.97 .10 0.01 0.935L04 0.03 0.97 1.10 0.0 0.6L04 0.03 O.S .10 0.0Q 0.96L04 0.03 0.9" 1.11 0.03 0.L04 0.03 0.9 1.11 0.08 0.96L05 0.03 O.3 1.11 0.08 OKL05 0.03 039S 1.11 0.01 06L05 0.03 039 Lll 0.08 0.96

1.03LOSL09

10.10

1.11Lll1.111.111.11

12L12L12L121.13

13131313

0.170.170.170.170.170.170.170.170.130.170.1S0.180.1I0.180.130.180.130.1S0.1S

0.330.330.840.840.850.350.850.350.340.350.340.860.360.860.870.870.310.870.87

117 0.45 0.55L17 0.45 05LIS 0.46O 0.56

B19 G.6 036L19 0.46 0.561.20 0.46 0.56L20 0.46 0.56L20 0.46 0.57L21 0.46 0.57L20 0.46 0.571.21 0.47 0.57L21 0.47 0.57L22 0.41 0.57L22 0.47 0.57.22 0.47 0.57

L22 0.47 0.571.22 0.47 0.57L22 0.4? 0.58L22 0.4? 0.53

L451.45L47L411.481.49L49L49L49L49L501.501.511.511.511.51L52L521.52

0.62 0.380.62 0330.63 0380.63 0390.63 0390.64 0390.64 0390.64 0390.64 0.390.64 0.390.64 0390.64 0390.64 0390.64 0.400.65 0.400.65 0.400.65 0.400.65 0.400.65 0.40

L62 0.06L62 0.04L64 0.04.64 0.04

L65 0.04L66 0.04

L66 0.03L67 0.031.67 .031.67 0.03L67 0.031.63 0.031.68 0.03L69 0.031.69 0.03L69 0.03L69 0.03169 0.03L70 0.03

Nets. 7 .4 JC ,, 7 .d u 2 S% h N t m m e n r t u A ae d u t ff l u c .e mtbu e e f se d l e M ~ O t ht w u ~ e 2 5 % e s u i h ( C *w o e p a f f u u c i o m , es tp o p.1 " a mCr- CeftIl ft.t y(sM MC)

3- ftwida doqiul affluh w1tm&

I~ st mi arhib d a~ 4 at b _ otllf t fl u K w l u e m w o e g 1. f 1 0 & m 4n2 5 & p w w m til . C V u. h m i s a i n e ald a e t f in e a n o d w a i u n g1hib.a f.byU9m'bArfld t mtaOldpeit

HM 1- 8Sldd dVbht;" OMOT0860d9 to f IO*paueffh% CV. (SAN - 0.03)SAM I SAudard deAlatlom onmsdg to e ba 25&pmeft CV. (AS - 017)

US ~lA^ IhlW uIn.tnutSA91 -ta1d l dA .ee _ wmvaagb toG75*p ee CV. (SA", 0-45)

S,- Stadurd eeaepu di90'pe h CV. ^(SA. -0.62)CV - Coo cimit fof tl orf r lwaLues.

UBPA 2000.U.dudt.d jeehg As MefATysNalPe htDrmiomrm IEA-33R40403.US hubbltPtAob Am4y.Ckdui O}L

7074,f


Precision of Endpoint Measurements

Sodium Chloride Chronic Reference Toxicant Datafor Ceriodaphnia dubla


Test dab Control Control Mean Canumber T6mydae ReproducUon

fDr Control Man() (offSpdngefmat) Relrdaction

(O Dfepa) I

CV CT MSD PMSD Crfir Control

('6) ReroductionCV(%)

(-A) forPMSDDeK)

I

.. . 234567891011121314is1617181920

12-18-02 10001.07-03 10002-04-03 10003-05-03 10004-11-03 10005-06-03 10006-04-3 10007-08-03 10008-05-03 10009-10-03 10010-07-03 100114-03 100124-03 10001-06-04 10002-03-04 10003-03-04 10004-06404 10005-04-04 10006-08-04 10007407:04 100

30.533.232.328.726.327.625.929.033.329.333.431.030.430.630.729.028.132.532.933.3

31.932.031.230.229.829.229.229.629.630.030.030.130.130.130.130.030.130.230.4

7.47.08.15.16.210.85.911.66.64.38.07.39.74.47.27.86.26.46.86.3

7.27.56.96.77.47.27.77.67.37.47.47.57.37.37.37.37.27.27.1

2.92.92.73.52.53.22.63.24.73.13.22.63.63.52.73.02.83.33.02.6

9.48.68.412.19.611.510.110.914.110.79.58.311.811.58.8

10.310.010.19.07.8

9.08.89.69.69.99.910.110.510.510.410.310.410.510.310.310.310.310.210.1

Note. CV- CoefdEniont o atdionfrisconkol prodction.On rage, twe CV for control uproduction Is 7.1% in Eviourncntal Testing Solutions, c= Cadodaphnda ronic. xidtylests.Lawr CVbound deteminedbytUEPA (10 percentile) - 89Y.

Upper CV bound dtuminedby USEPA (Cepercentile) - 42%MSD - Dinnm Significant Difne

PMSD - PercentM mSignificantD i=cM is azne of6:strcision. The PMSD hie n pe t diffErerce betwen e Control and utnen tdia can

be declaredutaisaflsignificantina ueeffluent iodtyts Onavcge, asigificantdiferene occursforEnLvroen Testing Solutons, In. c ionic toxidtyts swhn e olocant educes Corodaphnfa reprodactionby l0.1%flneote controLLowerPMSDbounddete inedbyUSEfPA(10 pcrcentilc) - II.per MSD bound de edbyUsePA(9'patil) - 37

CT - Central Tmndancy (Me Control Reproduction. CV, or PMSD)

The lorand pperbounds wr calculated by di USEPA sng 393 tests conducted ftom33 lboaories ftrCwitodaphnia ieproductionin chronicvtcrc= twicaut tests

USEPA 2000. Und ndig and AAccunig fr oMethod Vaiaity inWhole Effluent Todry ApplctionUnder& the Ntioad PofluntDiaclargeEimiiation PropL m EPA-33-R-O4.003. US Evonm==entd Protectiona Ancy, icinnati, OHL

07-07-04

1.8

1.6 .

0u)

U

t..

1.4

12

1.0

0.8

Environmental Testing Solutions, Inc.Sodium Chloride Chronic Reference Toxicant Control Chart

for Ceriodaphnia dubiausing Moderately Hard Synthetic Water

.

..

--------------

.................................. .............................................. .... ..................... .......................... ... .. .........................................................

.. _._...._._._...._._...._._._...._._...._._._...._.._.._.._.._..

..... . ..._ ......... ... - -.-...........-........................_..... .. ... ......... _..

............. ................ . . I .. I. . . .............................

0.6 1

0.4

02

Test date

7-day IC2s= 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a25% reduction in Ceniodaphnia reproduction for the test population.

-- Central Tendency (mean IC2.)

Laboratory Warning Limits (mean IC2. * S. 10 ,I SALIO= 0.08)

.Laboratory Control Limits (mean IC2, " SAM I Sj, - 0.17)

_- USEPA Warning Limits (mean IC2. 1 SA. 75 . SA 7 = 0.45)

- USEPA Control Limits (mean IC5 t SAM 9, SA.90= 0.62)


Ceriodaphnia dubia Control Reproduction and Coefficient of Variation

In Sodium Chloride Chronic Reference Toxicant Tests

40

35C

4J

4

C

E'h-

a

.I

- - - - , , ...... -.. , , , ......... . ...

........................... ............................................. ............

......... ........30

25

20 _

15 _ . _.. . _ . . . . .._. _.. _ ._. _.. . _.. _ . _. _ ._ . .. _ .. _ .. .. .. _ ._ .. _ .. _.. _ _-Minimum Acceptance Criteria (15.0 offspring per surviving female)

I I I I I I I I I I I I I I I I I I I

0

fC 0

o ,0 -

*c.q =

40

30

20

10

North Carolina Acceptance Limit (< 40.0°%)

Kentuky Acceptance Limit (C 30.0%)

.................................... ..........................

.......... .......... M.......................... ................... .........................................

I' I. - - -

seI °slgy0~~~fi%¢3oX:~tts436 sp

Test date

Control Reproduction or Coefficient of Variation (CV)

- - Central Tendency (mean Control Reproduction or CV)

... Control Limits (mean Control Reproduction or CV + 2 Standard Deviations)

Environmental Testing Solutions, Inc.Precision of Endpoint Measurements

Sodium Chloride Chronic Reference Toxicant Control Chartfor Ceriodapihnia dubia

using Moderately Hard Synthetic Water50

45

40

35

0

1C,,a

30

25

20

15

10

5

0tA.00%.0

Test date- PMSD - percent minimum significant difference. PMSD is teM minimum significant difference

between the control and treatment that can be declared statistically significant,- - Central Tendency (mean PMD)

. Lower and Upper PMSD BoundsLower PMSD Bound (10' percentile) - 1I1%S, Upper PMSD Bound (90' percentile) - 37X%(Lower and upper PMSD bounds were determined by USEPA for the method and endpoint)

1�I1�.

II12I.L[tI:.:LI

I

Environmental Tesilng Solutions, Inc. e TPage 1 of 6-

* Sodium Chloride Chronic Reference Tozicant Test(EPA*821-R-02-013 Method 1002.0)

Species: Cerlodaphnia dubla

CdNaCLCR #: -Z'L

Dilution preparation Informoton: Comments:Naa CHM Imbw. CK I tO__Stockppamio 10 ICOgN aIL/(dissolve50gNaCIin 500S

ddonlzed war)Diution m fiL) 600 g00 1000 1200 1400Stockvolume (mL) 9 12 15 1S 21Diluent volue(mL) 1491 1483 1415 1482 1479Total vohme (mL IS00 1500 1 1500 500 1500

Test organism Informatlo : Tet nformation:Organism age: < 24-hours old Randomizing template: P2PDate and times organisms a- OV'-0 O C T Of-( Incubator number andwere born between: elfloca[on: Z C.2Organism source: o042A 04 A-E YCT batch: ABS 7 TTransfer bowl information: pH -120SU Temperate 'Z'C Selenastrum batch: ABS ob

Daffy renewal Information:

Day Date Test Initiation, Control water batch Analystrenewal, or used

_termination time .

0 _ _ _ _ _ __4 iI25~ Aic o~1 on.e.o'i 110 onOo.oq2 I Io 0*o40 0 A

2 Dl.Nm*# 10,41 l -d

3 (7.10-0 103NR ai .(a4 CnII .o4 10____ 0o4o0 o' 4

-, -

As 5 oA,8Z - +I\Z .04t6 bR-FIB4t los'sff

Control nfzsornaton: AccW=critcd Summar of ett ndpoints:% of Male Adults: f, o s20% 7-day LC50 )'IqcOY. Adults having 3u Broods: /0017 a: so% NOEC 100n% Mortalityr Of s 20% LOEC 1200Mean OffspringiFemale: I33 -.3 15.0 offspring/fema1e Ch V JI .q

.CV: <40.0% j IC25 1035.1

I>.;: .Envirl

I:I .,t:.'.'

I.

I'

onmentul Testing Solutions, Inc.

Species: Ceriodaphnia dubia

CONTROL

Page 2 of 6

CdNaCLCR #: 2

Survival and Reproductdon DataReplicate number

Day I 1 2 | 3 41 S 6 7 a 9 10I Young produced 0C I O I Cb I ).I O I.OC| C)

Adult mortality L. '| L L_ L L7 L. E I2 Yeung produced _ _ .0 _ 0 C 0 . .

Adult mortality L - L . .1 L _3 Young produced f Q CL

Adult mortality L

4 Young produced 5 3 . I _'isI Adult mortality L L. t t| 1s Youngproduced c 1 0 |Z 10 Ls *3l . .

Adult mortality L-L 1 = l T I L6 Young produced ( ) |l a C) 1 0 D 0 |

Adult mortality | L. L L- .1 1|.l- L.

7 Youngproduced |'I 1|S I 9it 'I i 8 jj iS 1 I'T1IiTotalyoungproduced |34 |30 31 |$ 5| . 35 i3 31 | |FInal Adult Mortality | l I t t? i. |J ||

X for 3^Brood s S J | e g -X | 5| < r~ f

Concentration: I| %Mortality: I ^ |

Mean ringFemale: t3.3

600 mg NnCllL Survival and Reproduction Data- | Replicate nuober

Day 1 2 3 4 S[ 7 a 91 lI Young produced 0 0 |_ C

Adult mortaity L tl\ltl W - -l-|t|t2 Young produced D ) ) . 0 F| ) .. t ) .13 . 0 o

Adultmortality U _|I L |L- e ILIL3 j Youngproduced C) 0 C o 1 Q i V - o - - - I -

Aduitmortality L. L- L- l4 |Young produced I4 q IqISISI

Adult mortality I l. L- L L I Is Young produced 1

.Q _~I .Adult mortality .. L. C L C .(C CS Young produced 0 IC|C) 1 C I1

Adult mortality |, L- | L | -| |- -- - - - - - -7 Young produced 1 2o i- I-.L' Itr ao0 16 Is~Totalyoungproduced W| 3 | |4 | 33 3S 56 3|S | 5 37 | 36

Fina) Adult Mortality | L | | | I * L |

f CAk1 asve

|Concentration.-|% Mortality- MO

IMean Offspring/Female: I A3 I% Reduction fiom Control: -lt

of 6... _MMM

Species: Cerlodaphnia dublaCdNaCLCR#: Z. .

800 mn NaCIIL Survival and Reproduction DataReli-cate number

Day 11 2 3 4 5 T 7 a 9 10I Young produced 01 0L

0 C - .. n 0 1 0 T Loo

Adult mortality %- - > 12 Young produced 0 O lo t O C. ) n O

I *A L - - - L

3 Youngproduced tic Q crtal- - -

4 Young produced Ln lo S .Adult mortality L .Z-..~.~

S Young produced * * I I I O1Adult mortallty . t U t _ U U - U

. Ynng produced _I _ _ _ O 1 C)OAd ult mortality L L- L- . '-.. 1 t*

7 Yet a&produced Ig i's IS |( ZC II a I'sI ZO I 11T6lyoung produced 35 32 .5L Q.3 0 0 .s 7- 3 T1 |%

FinalAdult mortalty 1 L. L L L -t: ? L. 1

|Concenratifon:a % MdaityL 0 I| Mean Offspring/Femnale: 33.6 |

% Reduction from Control: -O.RV |

1000 mg NaCIlL Survival and Reproducton Data| - ~Repcte number - -

Day___ I 2 3 4 5 f 7 3 9 10I Young produced

Adult mortality L L. i U. T Z L | |

- -2 Young produced *0 0 T O I O I

Adult mortality L L TT L.- L I L

Adultmortality ugl I4 Young produced | __ || I 3 I

Adult mortality L L- I| L* L|

s Young produced | 1 | j | | 1 | 10 Ib

Adult mortalty L. .. L-. L- L- U -s Ymung produced C 0 0 |

Adult mortality L , . . I - --

7 Youngproduced 1N |- _ i | | 1 1 to|

Total young produced VI 32c0 -2. Zi - 5 1 316 Z, -3'( 3L 350Final Adult Ifortality . C t? T C ?

x CAM 61e41

|Concentraion |%Mortalit 07

| Mean Offspring/Female: I .So.° |

% Reduction from Control: 1.'. |

.Environmental Testing Solutions, Inc. Page 4 of 6

Species: Ceriodaphnia dublaCdNaCLCR #: Z(

1200 m2 NaCI/L Survival and Reproduction Data- Replicate number

Day 1 2 3 4 5 f 7 1 9 10I Young produced | o | 0 I O 0

Adult nortalty|t

2 Young produced i.{ L..._ . - - -Adult mortiit

Adult mortality 4. j L. % . .- %.-~

3 Young produced S0. .. L f 5 a.O..I.). Ž.Adult mortality L- ' Cs Young produced CI . O 1 0 0 S O C)

Adult mortality I| L u4 Yeung produced | Z - I 3 35 -O3 0

Adult mortality |. %_ C_ L~ - |. -

7 Young produced '- to I 0 O

Adult inortdlity L _ U U_ l.W U. i- '

Toa young produced I 1- L- 1 12. 11 4Tebl yo ng produced iz 'z 2 O 2 Z j £' Q 13 6q @ |Final Adult Mortality L | Z 1 L- t r

I Concentration:% Mortalit CyI

. Mean Offspnng/Fremale: I It . 2. |% Reduction from Control: |A .A1Z I

1400 mg NaCI/L Survival and Reproduction Data- - -Rep - cte number -

Day _ 1 2 3 4 5 6 7 a 6 10I Young produced | .. | O

Adultmortallty U | L U

A -lt--ortality L L L23 Youngproduced _& J O 1 0 1) Q o Q

Adult mortal I L i7 T - L L L L L

| Young produced - - -5 -I-

A|dult mortality |L L L | U_ | L L .

Adult morrhlity | I I|

| Concentruono UAu mMooayty: |I .U|

a

| t i4I oGdc4

I Mean Offspring/Female: I 'I.'I II % Reduction from Control: I &5 .5% |


Verification of Ceriodaphnia Reproductfon Totals

ControlDyROtC nmuber

1 2 1 3 | 4 5 1 6 7 9 10 T

I 0 0 0 0 0 0 0 0 0 0 02 0 0 0 0 0 0 0o0 0 0 o3 0 0 0 0 0 0 0 0 0 0 0

5 3 5 4 4 4 4 4 4 3 405 10 12 0 12 10 13 14 13 11 10 1056 0 0 10 0 0 0 0 0 00 107 19 15 16 19 19 1 1520 19 19 17S

Total 34 30 31 - 35 32 3 37 34 32 333

600 mg NaCI/LDay R~e at. amrberTO

1 2 1 3 1 4 5 1 6 75910 ao9

1 0 0 0 0 0 0 0 0 0 0 02_ 0 0 0 0. 0 0 0 0 0 0 03 O 0 O 0 0 0 0 0 I 0 04 4 5 4 4 4 6 5 4 5 5. 465 it 13 0 12 13 11 10 13 121 12 1076 0 0 10 0 0 1 01 01 0 1 0 117 17 152017181820161519 175

Total 32 3 343 35 36 35 33 32 36 339

800 mg NaCIIL

- 3 - - - -3 4 4 a 9 l

1 0 0 0 .0 0 0 0 0 0 0 02 0 00 0 0 0 0 0 0 0 03 0 0 0 0 0 0 0 0 0 0 04 4 64 6 44 54 44 455 13 11 0 10 14 12 1 1 10 13 10 1046 0 0 11 0 0 I 0 0 0 0 0 17 18 is 15S 20 161 19 18 18 20 17 176

TOta 35 32 3 36 34 3 34 32 37 31 336

1000 mg NaCI/LIa Repnt Rwbe TO

1 2 3 4 5 6 7 8 9 101 0 0 0 0 0 0 0 0 0 0 0

2 0 0 0 0 0 0 0 0 0 0 03 0 0 0 0 0 0 0 0 0 0 04 4 4 4 5 4 3 5 4 435 10 10 11 10 10 13 9 12 10 10 1056 0 0 0 0 0 0 0 0 27 14 15 17 15 1518 13 19 17 16 159

Total 29 30 32 29 31 36 26 34 32 30 309

1200 mg NaCI/L| SY | phl lcae unmber TOW

1>6|11 2 3 4 5 1 6 1 7 3 9 1101 0 0 0 0 I0 0 I0 0 0 0 02 0 0 0 0 0 0 0 0 0 0 03 0 0 0 0 0 0 0 0 0 0 04 0 2 0 3 1 0 3 3 0 0 125 1 0 1 0 0 3 0 0 0 0 56 4 4 2 5 0 0 6 0 5 0 267 7 6 7 1 4 10 5 4 6 37 7 5

Tota 12 12 10 12 11I 8 i 13 9 8 7 102

1400 mg NaCI/LDay RepTcTte nTmber Total

1 T2 3 4 5 6 -7 -1 0 0 0 0 0 .0 0 0 0 0 02 0 0 0 0 0 0 0 0 0 0 03 0 j Q 0 0 0 0 0 0 0 04 2 0 0 2 1 1 0 0 0 2 85 0°0 4 1 O 0 o O O 56- 1 51 3 4 0 0 0 1 0 0 147 6 0 3 4' 0 1 1 2 3 2 22

TOtl 9 5 10I 11 1 2 z 1 1 3 3 4 49


Chronic Whole Effluent Toxicity Test (EPA821-R-02-013, Method 1002.0)Species: Cerfedephnia dibla

Quality ControlVerification of Data Entry, Calculations, and Statistical Analyses

Tat amuben CdN*C1C #53 ( A J3epot SL)

Tet das ly 7-14, 2004 Reewd ___ ___ __ __ _ _ _

Co hatle Repikcate Rwbe Su. rv AvmV red- i.(HIL NS0 1 2 3 4 S 6 t (%) (effiprfugffule) m (%) "Nov)

- - - a- --- -

Com"l9 34 30 31 35 32 35 33 37 34 32 I"0 33.3 63 Not applkabk

6" 32 33 34 33 35 36 35 33 32 36 1I" 33. 4.5 -1J

n 35 32 30 36 34 35 34 32 37 31 1" 33. di -. 9

10 29 30 32 29 31 36 26 34 32 30 I" 303 9 7.2

121 12 12 10 12 1 8 13 9 a 7 10 10. 20 69.4

1400 9 5 10 II 1 2 1 3 3 4 1" 4.9 76A a3

Dpwwo M5Q "FMSDh

2-9847J

MSD- Mtmwn Siipcant DifaencePMSD- PermK Mbilmwn Slgidflamft Dfemmc

PMSDhsamesoftstpreldm Thet!MSD demtumn perce difecbetwe dcntrolid betmntw tcbe dece stkaclyifiCntl a vwole effluent tacty le Onawg, a signfificat diffe occur 1fbr EvineTa Tstn Soldoeu. dwcn lmdty t tsswbtfnafodcat redduce Cwedaphnizmducdoibyl.% floatecontoLLowePMSDbo dete dbyUSPA(Iercent) -11%Upp ?MSD bvmd dmIned byUSEA (9 pecetk) - 37Y.The lower a uppboms e calcuatd bydie USEPA Wng_ 393 tts condtel 33 biwoe for C a reodctio mcinc

c tee ofnt t

USEUA. 200Q Uud nd A IbrMetd V-blt Wole EuetTxityAUdtreNaoml ntDdp Elbno EA334 03. US Envhro tal PrtctionArg , Cindti. O


Ceriodephats Survivl mid Repd ifon Tt-RrdetoStit D| ; ; 9 Test 1d CdNCICR S he ID: R RefTaxcvntEnd D : 7/142004 Lab lD: TS-Envf. Testing Sol. SaITy e umcLbPotswi clorideSan& e Date: Fktooml: CHRON7I47A-821-R.02.013 Test Spies CD-Coaa dubiaCormments:Cone-ug/L 1 2 3 4 5 6 7 8 9 10

D.Contr.O 34.000 30.000 31.000 35.000 32.000 35.000 33.000 37.000 34.000 32.000600 32.000 33.000 34.000 33.000 35.000 36.000 35.000 33.000 32.000 36.000800 35.000 32.000 30.000 36.000 34.000 35.000 34.000 32.000 37.000 31.000

1000 29.000 30.000 32.000 29.000 31.000 36.000 26.000 34.000 32.000 30.0001200 12.000 12.000 10.000 12.000 11.000 8.000 13.000 9.000 8.000 7.0001400 9.000 5.000 10.000 11.000 1.000 2.000 1.000 3.000 3.000 4.000

Trunrorn: Untrantforaed 1-Tailed leatomacCsuc"ng/L Mean N-Mean Mean Min Max CV% N t-Stat Citical MMD Meal N-Mean

13Cxntrml 33.300 1.0000 33.300 30.000 37.000 6.339 10 33.600 1.0000600 33.900 1.0180 33.900 32.000 36.000 4.495 10 40.531 2!287 2584 33.600 1.0000800 33.600 1.0090 33.600 30.000 37.000 6.758 10 -0.265 2.287 2.584 33.600 1.0000

1000 30.900 0.9279 30.900 26.000 36.000 9.083 10 2.124 2.287 2.584 30.900 0.9196*1200 10.200 0.3063 10.200 7.000 13.000 20.565 10 20.441 2.287 2.584 10.200 0.3036'1400 4.900 0.1471 4.900 1.000 11.000 76.633 10 25.131 2.287 2.584 4.900 0.1458

AdiiHrM Tedst Statistic Critical Skew VintKohnnagrrD Ted hiats nomnl diulfrttion (p > 0.01) 0.877379 1.035 0.35713203 -0.2168864Badtlett' Tet indices eqal viances (p - 0.14) 8.24163818 15.0862722Hypetheds Test (1-ta% 0.05) NORC LORC ChV TJ MSf MSB MSE F-Prob aftDimnven Test 1000 1200 1095.44512 2.58407194 0.07759976 1756.42667 6.38518519 4.1E3-37 5,54Treatments vs D.Contro

07-07.04


Ih-r ftemlad- (29 Rmie")-POW MOL SD 95% CL Skew

^ .......

IC05d0o

IC15ICPM

I=251040IC50

924444441006376811022606?

108.84051055.072461103.768121136.23188

52.6343121.7M30743748117327.69035637.123111625.818694

819.245879935.2138161005477311022.73855104136132109253456

1003.91331019.1912410344666910MSQO M1065.191731I12399M

11453017

0.1971-L622440.9067-0.3027Q3090

-0.2419-0.0779

0.3

0. I

0.75.43527524 1125.10246

0.6-o0 s5

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Dos mL

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07-07-04

Envlronmental Testing Solutions, Inc. Page S of 6


Species: Ceriodaphnia dubla

Daily Ckemistrv:

CdNaCLCR M Z:

ow fl~ A fee&&tIl e9 io&ft.'UI Dav IU I

I o 1 I12 1 ofCLR I

1 I 2 I,,U

Analyst IConcentration I Parameter

pH (S.U.)DO (mg/L)

CONTROL Conductivity(umhos/cm)

-t

9AMkaunity*(mf CaCOL)_t o _ , _

Hardness(Mw CaRCO/L) a

4'Temperature 2S I . t I W 0 II | .2.z 74.8

INm -- - 1IpH (S. .) 1 .o0 IJS4 I V7s! I lT.1 I Iin^I IL 1 T- 7L I a I AI vJvJ ILWJA.]

600 mg NaCUL Conductivity(Rmhos/cm) 1 cZ

,I 31

24 .i

T .'.

r1qo0LS z-?Temperature

('C) 2S3. I I z4.6 I z4 . IpH (S.U) 7 0 s . .4 . ;90DO (mg1L) w 6.69> t;t 1

800 mg NaCUL Conductivity(jgmhos/cm) 1 S 0Temperature Z5 LB

pHSHU. 7. / 7 7 7.55 _ SS tDO(mWL) t 7.6 .7 V.V _ 78

1000 mig NaCI/L Conductivity(wzhoslcm) _____

Temperature 2ZS zSZ Z ,1rc)PH(S.U.) 1_7°_ ?. T6 S. s 7.qE1 . .a IDO (nmgL) i t 6 7.7

1200 mg NaCI/L Conductivim y ) 5° 2qtZ(g&mhos/cm)VtTemperature 2I'2 Z4.q ZZ Zs.i

C. _ _T _ I VA Z4. _ _ _

OH (S.U.) .4o I 4.60 1 7.ss. 1 .146 1 7. TDO fimngL)

1400 mg NaCVL Conductivity(;L )

Sooo g PWS. *TiI SqI 7( 7.o4

Z5lTemperature1C) I 24.¶1 I Z11.9 I

STOCK Conductivit I 1 JI D_ _ _ _ _ _ _ _ _ (Ig m hos/cin _I 000

__ __

MWE_niIa Ial

Ini ial I I itial - I -- F i -al l- - Ii ti-a Ij Final I

Environmental Testing Solutions, Inc. Page 6 of 6Enirnena TetnouinIc ae6o

Species: Ceriodaphnia dubia

CdNaCLCR M 2:

Analyst _ Ana . __&4.Concentration Parameter

pH (S.U.) 13-12 gi7l It Z.2A#DO (mgL) >. l 8.0 6

I CONTROL Conductivity A 9 O

Alkalinity

Hlardness

'Temperature

.pHI (S.U. 909<1 "o o 8-17- e.as exit 1BoDO (mg/L) e B Z.z -L . 6So

600 mg NaCVL Conductivity

. T scmpe )tr 1ZS. MO1 2- co . ;wTemperature X. b 2 2.O 75.0 . tt g

pE (SU)ZS0Q f 1 3t .D 8.1 31 0 q.q11 1q~

IpH _______ e i7-ex

: DO (mgL)8. I800 mg NaCUL ConductiityZ

Temperature 25 ?A.6 2'(.1 2S.0 I25. Z 1 2q! *

pH (S.U.) I- 4 3jJ - __ -___1DO (mg/L) ___8~. 6. . 8

g1000 mg NaCVfL Conductivity

Temperature 2.S 1z4i 2l1 24 7.I L. MS 2.5.ca.- 2-6 -+ Z. -;2.

1200 mg NaCIL

pH (S.U.) 5.ln BZ49 I 8so.. I 5.1 I ggz~ I I.' -7J'16 I.,_ _ _ _ _

DO (mg/L)Conductivity(pmhos/cm)Temperature(00C

ZS. O

.J9

zwoo MM

7q.-I I zq.j 4A 25-

_8 6.1 8 sl

2. q1 I 2qt

I 1.0

I II _- _ _ _

-. - r . _ _

Big (S.U.) 5-a- I QrLn I q.o7 I f.10

1400 mg NaCVLDO (mgAL) I -:;.e - _-_ . . _ ._

Conductivity(sLmhos/cm)TemperatureCC)

zoqc2q. L q t- 7 ZQ

. Final _ Inltial Fl

2q. 4 2.q zq.(aA I

~OK Conucivity1Initial

I I

Initial I Flail IIIitia

PERMITTEE NAME/ADDRESS (Incude FecilfvNarnieocat8on DfflmName TVA - SEQUOYAH NUCLEAR PLANTAddress P.Q. Bo XO200 0 - _________

- -- yQ mmlifSY T 37384 - - 7---:d _ _ _AHNUL -& 3 A84pdjhf_ _MA -SEgQX,5jH tXARO___Ti Q_&51UOCU __________ -- -- --

NATIONAL POLLUTANT DISCHARGE ELMNATION SYSTEM (NPDES) MAJORDISCHARGE MONITORING REPORT (DURJ (SUBR 01)

! TN0026450 101 Y I F - FINALI PERMIT NUMBER I DISCHARGE NUMBER ANNUAL MONITORING (PCBS)

| MONIRING ,PERIp | EFFLUENT

Form Approved.OMB No. 2040-0004

ATTN: Stephanie A. Howard From 03 08 |01 To | 04 | Q 7| 31 | NO DISCHARGE [ InuOIE: Read hisftucfton befrm co"

NAMEtTITLE PRINCIPAL EXECUTIVE OFFICER.4

J. Randy Douet

Site Wice President

I Cmf und Puy cil ahmWadalleStedvnuw t. _er I _dermWdirswoor supmviom hI a w~h a yslsm deftd as t d perm

pmp" r aend mW vluata Vi titon Kubmgtd Ban mvy Iqtft of en pato or 4 4wtlPW5 who mwmp fi SYMM o ion PWWW *5tIyY re for sph Oe n Principal Environmental EngineerWa.f Il dt.W U is,toI boo of nrW awfwldge vfWd bed Mm8,

mat, aid comnp. Imae Vioitaagrica penles rfor abmhfiW SIGNATURE OF PRINCIPAL EXECUTIVk i ew rdngpoasIbid fawyidhspd- bl brl ba OFFICER OR AUTHlOR17Fn AGE-NT4,

TYPED OR PRINTED I - -- . ... - .

LNIYIMNT IANU LAELANATIIN C)F ANT VIOLATIONS (RleMencq . aftacmden hqM)

--- . .... I jIs J r u w_ ma an use Page 1 o._1EPA FOrmll 33Z01 lRE-V 3m} "Umsus eamons may De tlse Page I of I

PERM TFEE NAME/ADDRESS (Inchlde FslfNrrmedocaln if DiffeentName -A SEQUOYAH NUCLEAR PLANTAddress E.Q )C290

_ __ IfNROFM1E SSB-E _ __ _ _C -- -- - -AS -TN -7e

Laty.... Y -_ASEQJJ OAH NUCLEA E3L8jLoca__ HAMIL TOCQUNY ___


NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAMORDIRCHARGE MONITORING REPORT IDMR_

Form Approved.nunt I- - -n

(SUBR01) JMOr

TN026450 103 G F - FINALI PERMIT NUM E [CHARGENMBER LOW VOL. WASTE TREATMENT POND

TV. Suuw

ff - --- ffi - - |

RIai~rrf%0I1f1 oco~lfn l EFFLUENTI rull1 nn --- vL

From T 04 o 07 | 01 | NO DISCHARGE [ 0NOTE Read Insuctn befo complet this forrm

PARAMIETEiR QUANTITY OR LOADING QUAUTY OR CONCENTRATION NO. FREQUENCY SAMPLE__ __ __ __ _ _ __ __ ____ __ _ __ _ __ __ __ _ _ __ __ __ _EX OF TYPE

AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS

PH SAMPLE 7.0 8.7 12 O 13131 GRABMEASUREMENT

000 *7~a ~~OY> SU ~ FIER ~T

EFFLUENT GROSS VALUE . .. . .SU E '

SOUDS, TOTAL SUSPENDED SASPLE 159 219 25 16 19 19 0 4131 GRAB

00530 1 0 0 LSD ~ -- 711

EFFLUENT GROSS VALUE LBS/DY M GIL JX <

OILAND GREASE SAMPLE <48 <58 26 <5 <5 19 O 4131 GRAB

00556 1 0 0 LDS/DY MGIL '7 MM< 7m T'EFFLUENT GROSS VALUE 6 M VT DI~iX hA~ IbAILM~FLOW, IN CONDUIT OR THRU SAMPLE 1201900 . ~ 311 TTLTREATMENTPLANT MEASUREMENT 1.230 1.950 03 . 31/31 TOTALZ

50050 1 0 0 ROMGD T 0 A f OYM

NGROSS VALUESAMPLE

IMMAUREMENT

MEASUREMENT CT 7II7EM7NT ;

SAMPLEMEASUREMENTPLE RMT,

NAMEITITLE PRINCIPAL EXECUTIVE OFFICER I Cd_ todr Panaly of low tt tNis domd dard #A aad-w. Vepd Urrmy Ku TELEPHONE DATEd adlo- Nor srisim n hi adi ut, wi a systm designd to *s*a wstt qualfwd pwrarVn nW~~)<

J. Randy Douet PPIY gatherandevaluatthefWttan e b Baed enmyhdf taepon or

hk Vi Preton ae n 1ronneurn -mis.* to b s od my ofbowerdgs awid Wet, Mm, Principal Environmental Enghieer 423 843-8700 04 08 12ade V -n a, l e m s1 t dpentesfor lftfaie SIGNATURE OF PRINCIPAL EXECUTIVE J

TYPED OR PRINTED hgipoq d tO. Imd hr b r J OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR MO DAYCOMMENTS AND EXPLANATION OF ANY VIOLATIONS (Rernc. ad tachmnts here)

EPA Form 3320-1 (REV 31) Ptyus Pufs mayS bue Page I of I

PERMITTEE NAME/ADDRESS (Induds FacilitvNameocatn IDfDilerentNams TA - SEQUOYAH NUCLEAR PLANTAddres PO B) 00XJOO_ _ _ _ _ _

_ _ __ tfEROLF CE S- -2B - - - - - - - -____ODDY- DAISY__JN 3738L

Loclon _AMILTO COU ______NTY_

NATIONAL POLUUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OMB No. 2040-0004

[ TN0026450 107 G F - FINAL

PERMIT NUMBER DISCH GE NUMBER METAL CLEANING WASTE POND1

"nmrIT>ZImNNf nermlon I EFFLUENTIV"- Iullnp=u ,

YEAR I MO I DAY | YEAR MO D NO DISCHARGE [M INOTE- Read Ihstdlons before comnotina tIs form.ATrN: Stephanie A. Howard

PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SATMIPLE_________ ____ ________ _________- EX OF TYPE

AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS

pH SAMPLE 1MEASUREMENT 12

000 0 0 SUuMN1 ~~ "~

EFFLUENT GROSSVALUE - M U _ zPHOSHORU, TOAL (S P)SAMPLE -

PHOSPHORUS, TOTAL (AS P) MEASUREMENT 19

00665 1 0 0 MGILEFFLUENT GROSS VALUE - MGL $SOUDS, TOTAL SUSPENDED SAMPLE - 19

00530 1 0 0 "" ¾~ e*** .' MOS..iL;

OIL AND GREASE SAMPLE -*- *" *" *MEASUREMENT 19

-P **l*E** i*n** MGL00555 1 0 0 z~UIEE1 AALM>___

EFFLUENT GROSS VALUE -M

COPPERA TOTAL (ASCU) SAMPLE i19MEASUREMENT 1

0104 1 0 0 - MGII /2TL

EFFLUENT GROSS VALUE ,-. ;_:d . D IL MGQ _ ___

IRON, TOTAL (AS FE) SAMPLEN T ****.** 19

MEASUREMENT 1

01045 1 0 0 MOIL~; ss *.**

EFFLUENT GROSS VALUE R = MG/L ¶ b V

FLOW, IN CONDUIT OR THRU SAMPLE 03 _*TREATMENT PLANT MESUREMENT 0

50050 1 0 0 F R MI'O"1T MGDcmii77 aEFFLUENT GROSS VALUE MGD _ * m

NAMEmflTLE PRINCIPAL EXECUTIVE OFFICER I Cwu nder pmiIy d owf w M docum d t m rerd wI y _ |I TELEPHONE DATEdieciton orsupwisimionehwhIN codith.system degned to semw qt dpt ,lTELEP HONE DAn

J. Randy Douet sgr ed e n b Bed on my nqu dof w peon ork, hO d b ofti055 pifI md' diw w beK Principal Environmental Engineer 423 843-6700 04 08 12

Site Vice Poesident , amcm I ws e gnt pre fr dxug b e SIGNATURE OF PRINCIPAL EXECUTIVETYPED OR PRINTED ar-xn. khdard hw pmalfofflmipd ht i W bri b OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR MO DAY

COMMENTS AND EXPLANATION OF ANY VK)LATIONS (Reabtw aD atachments hem)

No Discharge this Period

EPA Form 3320-1 (REV 3/99) Phwiu edflom may be used Page I of I

PERMITTEE NAME/ADDRESS (ncude Fei#tvNinmocatnI HDifferent)Name TVA - SEQUOYAH NUCLEAR PLANTAddress P.O. BOX 2000 _ _ _ _ _ ____ PEN ERF IXSdLL&A ---------

S__ ODOY - DAISY_ Th3_ _______Fadjly_ _MA- SE ,AH NUCLEAfl TjLocton HAMILTO_CLN QUY ________

NATIONALPOLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJORDISCHARGE MONITORING REPORT (R) (SUBR 01)

I TN0026450 110 G F - FINAL

I PERMIT NUMBER I ISCHARGE NUMBER I RECYCLED COOLING WATER

|-_ _,_MONIRING ERIO EFFLUENT

Form Apprioved.OMB No. 2040-0004

ATTN: Stephanie A. HowardYEAR I LMOf IDAY oI

From i04 |07 O1 | ITo 04 | 07 | 311 NO DISCHARGE []NOTE Read Irtsbuclons beore Oomolelhis toftnm

PARAMETER QUANTITY OR LOADING QUALTY OR CONCENTRATION NO. FREQUENCY SAMPLE- EX OF TYPE

AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS

pH SAMPLEMEASUREMENT 12

000 I 0 0 ~ ~SuEFFLUENT GROSS VALUE _ U

____ m INmuJ -A-

SOUDS, TOTAL SUSPENDED SAMPLEMEASUREMENT 19

00530 1 0 0 MCIIIMN1L~~,h~ -'

EFFLUENTGROSS VALUE __ t - z _

OIL AND GREASE SAMPLE ***.*. ** 19MEASUREMENT 19

00556 1 0 0 MGIL ~ 1AL~e oEFFLUENT GROSS VALUE _ MGIL _____

COPPER, TOTAL (AS CU) SAMPLE .... .*aw*.MEASUREMENT 19

01042 1 0 0 ¶' MI. FEFFLUENT GROSS VALUE _ E~tIZEMIRON, TOTAL (AS FE) SAMPLE * DAL-X _____

MEASUREMENT 19

01045 1 0 0 ~' I-~~*¶

EFFLUENT GROSS VALUE _ MG/L " 7

PHOSPHORUS, TOTAL (AS P) SAMPLE * * *n* ** * ** -- 19MEASUREMENT 19

00555 1 0 0 *.*.

EFFLUENT GROSS VALUE , T7 - Y MG/L " AFLOW, IN CONDUIT OR THRU SAMPLE03aTREATMENT PLANT MEASUREMENT 03

50050 1 0 0 PERMGD _ UEFFLUENT GROSS VALUE MAG.AIY X ________

NAMEtTITLE PRINCIPAL EXECUTIVE OFFICER I C ypealtoatIsdo md a reped d tdrmy _ TELEPHONEDTrh nndm s do ttinswqualfedporl TELEPHONE

J. Randy Douet utsr - svs himsln bmtled sW mo InqukyOf d poncr or

Site Vice President . tWi I lo~lmbwran~dp~b" , Puincipal Environmental Engineer 423 843-8700 04 08 12~ sidom!tee.I m e Mst hee m sg~lflmwpealtesf~ ukltlngfase SIGNATURE OF PRINCIPAL EXECUTIVE . 4JLTYPED OR PRINTED thk poesslly odf finW nd htonwtfbr nwhigft OFFICER OR AUTHORIZED AGENT A EA| NUMBER YEAR MO DAY

COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reemch aIachnents here)No Discharge this Period

FA F hrm yFV 41D Pk...urn rue *n bout W ton v ad rrwtmffnnn lay Lo Usev Page 1 of I

PERMITTEE NAMEIADDRESS i7ncd FUclitV Nactln fDiffent)Name TVA - SEQUOYAH NUCLEAR PLANTAddress P.O.BOX 2000 - - __

SODDY - DAISY_ T 37384_d_ _VA E L. . . .

_tlp - M LTON OsU _kjLXv_________ -- --

NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJORDISCHARGE MONITORING REPORT (DMR) (SUBR 01)

TN0026450 116 G F - FINAL

I PERMIT NUMBER I I DISCHARGE NUMBER BACKWASH

| MONITORING ,PERIO . . EFFLUENT


From IDAY | F MOFrom 04 107 1 J1 To 04 107 31 I NO DISCHARGE =

NOTE! Rand khsteMns befam ammOMUM this brnn.ATTN: Stephanie A. Howard

PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE

AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS I

DEBRIS, FLOATING (SEVERITY) SAMPLE 9A 0 1/31 VISUALMEASUREMENT

01345 1 0 0 ' pq 1.iPASSNO . IUIEFFLUENT GROSS VALUE . FAILmIOIL AND GREASE VISUAL SAMPLE O 31 VISUALMEASUREMENT

84086 1 0 0 RT YES-1 M7- i -4EE -EFFLUENT GROSS VALUE N_-_ _ _ O z

SAMPLEMEASUREMENT

SAMPLEMEASUREMENT

SAMPLEMEASUREMENT

SAMPLEMEASUREMENT

SAMPLEMEASUREMENT

NAMEmTLE PRINCIPAL EXECUTIVE OFFICER CuIf du petdy o w dond m ettmntw pu d uad mny 1 TELEPHONE DATEdrcton or q ohawam In c-r lo Wfhaaydmo e toueVWqmIfedd b d pA .rs nAQJcl _J. Randy Douet pgtr id aate ftliun atbuited easd on my kuqry of toh pomn or M

Site Voft hb mbt~d myIoftbbodpWanm d b~ef, VPrincipal Environmental Engineer 423 843-6700 04 08 12'S Vnt acuade md c t. I mo mea ee me slgdi pats forb behr n SIGNATURE OF PRINCIPAL EXECUTIVETYPED OR PRINTED Ihh. m',kck eftpossbftq db"*pi=. b rlobwrhg visbi OFFICER OR AUTHORIZED AGENT EA NMER YEAR MO DA

COMMENTS AND EXPLANATION OF ANY VIOLATIONS ftawmqff d athments here)Operations performs visual Inspections for floatIng debris and oil and grease during an backwashes.

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PERMITTEE NAMEIADDRESS (Incude fault Narnmeocfton fDff~wertName __A - SEQUOYAH NUCLEAR PLANT ____AAdAsL__E £Q:2)C2000 ----____(INU&ERFICE SB-20 ____

___j SQDY AIS_ Nj~tw _______

Bca~on _AMILTONCQ U_______

ATTN: Stephan A. Howard

NATIONAL POLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJORDISCHARGE MONITORING REPORT MUM (SUBR(01)

LI TN026450 117 G F-FINALI PERMITNUMBER I ISCHARGE NUMBERl BACKWASH

I MONITORING PERIOD I EFFLUENT


YFARro MO I DU A YEAR L LMO DAFrom 04 T 07 T 01 I To 04 1 7 13i1 NO DISCHARGE GE

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_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ EX OF TYPEAVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANA YSIS

DEBRIS, FLOATING (SEVERITY) SAMPLE 0 9A O 1 /31 VISUALMEASUREMENT

01345 1 0 0 ,r~REQ* JIR MEWN 77 A YMO 7 i A7 . FAIL-I0EFFLUENT GROSS VALUE FAI_1 _ _ _ MOIL AND GREASE VISUAL SAMPLE 0 e***1131 VISUALMEASUREMENT

84065 1 0 0 *G 7i YESI = -

EFFLUENT GROSS VALUE TNORSAMPLE

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NAMEtlTILE PRINCIPAL EXECUTIVE OFFICER ICly wer pey or p W y bout docanmiid satad we' er wpddr my IE TELEPHONE DATEdbeon or superYin mi do wih a systav designed to eaxmm t qimtlfed peruae = -g-I

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t~nwtn.Vekftomutko Wmhite Ia . to do. bedat nrm lmaldg NW beter true 423__843_6700__04____12SVi.res and a.nqW I cm I mn soon p M thi wen algriwfcmg buetx d n f' SIGNATURE OF PRINCIPAL EXECUTIVE

TYPED OR PRINTED , hdIM h p d h - OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAYCOMMENTS AND EXPLANATION OF ANY VIOLATIONS (1me al atcmenb hee)Operations peforrns visual Inspections for floating debris and oil and grease during aD backwashes.

EPA Foffn 3320-1 (REV 31") PYVVIM edmons may be USW Page I of I

PERMITTEE NAME/ADDRESS llnduds Fad7 Nva4ocon fDffIeren)Name TVA - SEQUOYAH NUCLEAR PLANTAddK LP.O_. QBOX20W ____________

SODDY- DAIY TN 73e4fldft _[VX -SE2jJOYAHN UCEAR PLANT


NATIONAL POLUTANT DISCHARGE ELMINATION SYSTEM (NPDES) MAJOR IDISCHARGE MONITORING REPORT (DMRU

(SUBR 01)CTN0026450 118 G F- FINAL

PRMIT NUMER I DISCHARGE NUMBER WASTEWATER & STORM WATER

I MNITORING FRI --- I_ EFFLUENT

Form Approved.OM8 No. 2040-0004

YEomTMO YER |From 04 107 Q1 01 To 04 107 131 NO DISCHARGE [ 1

NOTE: Read bIstnucion befr complena this bnnm.

NAMEnTLE PRINCIPAL EXECUTIVE OFFICER I Cayudrpeayof lwia * downd alt diwswemwd td my |I TELEPHONE DATEJrdeeftcrsuipwvlsn bi a -dencwlfi asytem dahlgradto aswe tatq~miffed pu'aramm AQaAdAm-MJ. Randy Douet p s r w oakni ft blannebn nibittb Bda an Irq hquiry df b Pam or J

Site VRne President _ _ k O h _a __o'g b ",h b Principal Environmental Engineer 423 843-700 04 08 12sos, wnd ax. I ewrmetereme sla peneforSbblttlh fse SIGNATURE OF PRINCIPAL EXECUTIVE

TYPED OR PRINTED h . dh g poftltoy df few end hiir lsonl fbrv5kl5a. OFFICER OR AUTHORIZED AGENT EA NUMBER YEAR MO DAY

COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reftrence olf uffachnmens hee)During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfHl.

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tenrnessee valley authority. post office box 2000, soddy-daisy ... · dhee0n orupeision h ardieh...

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