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Amino Acid Assay Kits

Key Features:

• Non-radioactive• Specific, homogeneous assay• Convenient: minimal sample preparation; fast protocols (1-2 hours)• Cost effective: 100 assays; High Throughput Screening compatible• Validated: using mammalian tissues, cells, biological fluids

Tech Help: Email: [email protected] | Fax:408.493.1801 | 3

*C: Colorimetric; F: Fluorometric

Figures. Glycine concentration in human serum, urine and saliva was detected using K589.

Amino Acid Assay Kits

Target Cat. No. Size Sample TypeAlanine (C/F) K652 100 Assays Serum, cells, tissues

Asparagine (C) K736 100 Assays Plasma, serum, cell lysates

Aspartate (C/F) K552 100 Assays Serum, cells, tissues

BCA (L-I-V) (C/F) K564 100 Assays Serum, cells, tissues

Cysteine (F) K558 100 Assays Serum, cells, tissues

Glutamate (C) K629 100 Assays Serum, cells, tissues, food

Glutamine (C) K556 100 Assays Serum, cells, tissues, urine

Glycine (F) K589 100 Assays Serum, cells, tissues, urine, saliva

Histamine (C) K506 100 Assays Meat products, milk, yogurt, cheese

Histamine (F) K386 100 Assays Food, condiments, bio samples

Homocysteine (F) K531 100 Assays Human or animal plasma or serum

Hydroxyproline (C) K555 100 Assays Tissues, urine

Hydroxyproline (C) K226 100 Assays Tissues, urine

L-Arginine (C) K749 100 Assays Biological fluids, beverages

Methionine (F) K442 100 Assays Serum, plasma, cells, tissues

Phenylalanine (F) K572 100 Assays Serum, cells, tissues

Phenylalanine (C) K481 100 Assays Cells, tissue lysates, serum

Sarcosine (C/F) K636 100 Assays Serum, cells, tissues

Serine (F) K743 100 Assays Serum, cells, tissues, CSF

Taurine (C) K988 100 Assays Urine, beverages

Threonine (F) K463 100 Assays Serum, cells, tissues, CSF

Total D-Amino Acid (F) K445 100 Assays Biological fluids, animal tissues

Total L-AA (C/F) K639 100 Assays Serum, cells, tissues

Tryptophan (F) K557 100 Assays Serum

Tyrosine (C) K573 100 Assays Tissues, cells, serum, urine

Seru

m/S

aliv

a G

lyci

ne (µ

M)

Urin

e G

lyci

ne

(µM

/mM

Crea

tinin

e)

Serum Saliva Urine


Ferroptosis

Metabolism of carbohydrates includes the process where in complex carbohydrates with 3 or more sugar units in a chain are broken down into simple mono- and disaccharides like glucose and sucrose which are important components of several metabolic pathways.

Assay Kits

Target Cat. No. Size *Detection

Ace

tyl C

oA

Citrate Assay Kit K655 100 Assays C/F

Coenzyme A (CoA) Assay Kit K367 100 Assays C/F

HAT (H4) Activity Assay Kit K336 100 Assays F

HAT Activity Assay Kit K334 100 Assays F

L-Carnitine Assay Kit K642 100 Assays C/F

p300 Inhibitor Screening Assay Kit K346 100 Assays F

pCAF Inhibitor Screening Kit K345 100 Assays F

PicoProbe™Acetyl-CoA Assay Kit K317 100 Assays F

β-Hydroxybutyrate (β-HB) Assay Kit K632 100 Assays C

Chol

este

rol

Alanine Assay Kit K652 100 Assays C/F

Alkaline Sphingomyelinase Activity Assay Kit K987 100 Assays C

CETP Activity Assay Kit II K595 100 Assays F

CETP Inhibitor Screening Kit II K594 100 Assays F

Cholesterol Detection Kit (cell-based) K587 100 Assays N/A

Cholesterol Efflux Assay Kit (cell-based) K582 100 Assays F

*C: Colorimetric; F: Fluorometric; N/A: Not Available


Ferroptosis FerroptosisTarget Cat. No. Size *Detection

Chol

este

rol

EZScreen™ Total Cholesterol & Cholesteryl Ester Assay Kit (384-Well) K957 100 Assays C

EZScreen™ Triglyceride Quantification Assay Kit (384-well) K952 100 Assays C

HDL and LDL/VLDL Quantification Kit K613 100 Assays C/F

HDL Uptake Assay Kit K586 100 Assays F

HMG-CoA Reductase Activity/Inhibitor Screening Kit K588 100 Assays C

Lipid (Oil Red O) staining Kit K580 100 Assays N/A

PicoProbe™Acetyl-CoA Assay Kit K317 100 Assays F

PLTP Activity Assay Kit II K593 100 Assays F

Total Bile Acids (TBA) Assay Kit K209 100 Assays C

Total Cholesterol and Cholesteryl Ester Assay Kit K603 100 Assays C/F

Total Cholesterol and Cholesteryl Ester Assay Kit II K623 100 Assays C

Cyst

athi

onin

e

Cystathionine β Synthase Activity Assay Kit K998 100 Assays F

Cystathionine β Synthase Inhibitor Screening Kit K695 100 Assays F

Cyst

eine

Cysteine Assay Kit K558 100 Assays F

Glu

tam

ate

Alanine Aminotransferase (ALT or SGPT) Activity Assay Kit K752 100 Assays C/F

Alanine Assay Kit K652 100 Assays C/F

Alpha-Ketoglutarate Assay Kit K677 100 Assays C/F

Aspartate Aminotransferase (AST or SGOT) Activity Assay Kit K753 100 Assays C

EZScreen™ Alanine Aminotransferase (ALT or SGPT) Activity Assay Kit (384-Well) K941 100 Assays N/A

Glutamate Assay Kit K629 100 Assays C

Glutamate Dehydrogenase Activity Assay Kit K729 100 Assays C

Glutamine Assay Kit K556 100 Assays C

PicoProbe™ Glutamate Assay Kit K413 100 Assays F

PicoProbe™ Tyrosine Aminotransferase Activity Assay Kit K486 100 Assays F

Glu

tath

ione

EZCell™ Glutathione Detection Kit (Blue Fluorescence) K504 100 Assays N/A

Glutathione (GSH/GSSG/Total) Assay Kit K264 100 Assays F

Glutathione Assay Kit K261 100 Assays C

Glutathione Assay Kit K251 100 Assays F

Glutathione Peroxidase Activity Assay Kit K762 100 Assays C

Glutathione Reductase Activity Assay Kit K761 100 Assays C

GST Activity Assay Kit K263 100 Assays C

GST Activity Assay Kit K260 100 Assays F

Methylglyoxal Assay Kit K500 100 Assays C

PicoProbe™ Methylglyoxal Assay Kit K461 100 Assays F

PicoProbe™ Reduced Glutathione (GSH) Assay Kit K740 100 Assays F

Reduced Glutathione (GSH) Assay Kit K464 100 Assays C



Target Cat. No. Size *DetectionG

luta

thio

ne Glutathione Reductase (GR)(rat) ELISA Kit E4625 100 Assays N/A

Glutathione Reductase (GR)(human) ELISA Kit E4623 100 Assays N/A

Glutathione Reductase, mitochondrial (Mouse) ELISA Kit E4624 100 Assays N/A

Gly

cine

Glycine Assay Kit K589 100 Assays F

G-6

-P Glucose-6-Phosphate Assay Kit K657 100 Assays CPicoProbe™ Glucose-6-Phosphate Assay Kit K687 100 Assays F

HM

G-C

oA

HMG-CoA Reductase Activity/Inhibitor Screening Kit K588 100 Assays C

Hom

ocys

tein

e

Homocysteine Assay Kit K531 100 Assays F

LP

Lipid Peroxidation (MDA) Assay Kit K739 100 Assays C/F

Paraoxonase 1 (PON1) Activity Assay Kit K999 100 Assays F

ME1 PicoProbe™ Malate Dehydrogenase Activity Assay Kit K538 100 Assays F

Malate Dehydrogenase Activity Assay Kit K654 100 Assays C

Met

hion

ine

Methionine Assay Kit K442 100 Assays F

NA

DP+

6-Phosphogluconate Dehydrogenase Activity Assay Kit K540 100 Assays C

Ammonia Assay Kit K370 100 Assays C

Cytochrome P450 Reductase (CPR) Activity Kit K700 100 Assays C

Dihydrofolate Reductase Activity Kit K246 100 Assays C

Dihydrofolate Reductase Inhibitor Screening Kit K247 100 Assays C

EZDetect™ Aldo-keto Reductase Activity Assay Kit K847 100 Assays C

EZScreen™ NAD+/NADH Assay Kit (384-well) K958 100 Assays C

Glucose-6-Phosphate Dehydrogenase Activity Assay Kit K757 100 Assays C

Glucose-6-Phosphate Dehydrogenase Inhibitor Screening Kit K225 100 Assays C

Glutathione Peroxidase Activity Assay Kit K762 100 Assays C

IDH1 R132H Mutant Inhibitor Screening Kit K493 100 Assays C

Isocitrate Assay Kit K656 100 Assays C

Isopropanol Dehydrogenase Activity Assay Kit K698 100 Assays C

Microsome Isolation Kit K249 100 Assays N/A

Mutant Isocitrate Dehydrogenase (Mutant IDH) Activity Assay Kit K985 100 Assays C

NAD/NADH Quantitation Kit K337 100 Assays C

Ferroptosis



Target Cat. No. Size *DetectionN

AD

P+NADP/NADPH Quantitation Kit K347 100 Assays C

PicoProbe™ Glucose-6-Phosphate Dehydrogenase Activity Assay Kit K751 100 Assays F

PicoProbe™ NADPH Quantitation Assay Kit K349 100 Assays F

Thioredoxin Reductase Activity Assay Kit K763 100 Assays C

6-ph

osph

oglu

cona

te

6-Phosphogluconic Acid (6-PGA) Assay Kit K217 100 Assays C

6-Phosphogluconate Dehydrogenase Activity Assay Kit K540 100 Assays C

Phos

phol

ipid

(PL)

Cardiolipin Assay Kit K944 100 Assays F

Choline Kinase (ChoK) Activity Assay Kit K476 100 Assays F

EZClick™ Global Phospholipid Synthesis Assay Kit (FACS/Microscopy), Red Fluorescence K717 100 Assays N/A

EZClick™ Total Phospholipid Assay Kit (Cell-Based) K537 100 Assays N/A

Glycerophosphorylcholine Assay Kit K433 100 Assays C/F

Lysophosphatidylcholine Assay Kit K735 100 Assays C/F

PAF Acetylhydrolase (PAF-AH) Inhibitor Screening Kit K766 100 Assays C

PAF Acetylhydrolase Activity Assay Kit K765 100 Assays C

Phosphatidylcholine Assay Kit K576 100 Assays C/F

Phosphatidylethanolamine Assay Kit K499 100 Assays F

Phosphatidylserine Assay Kit K565 100 Assays F

Phospholipase A2 Activity Assay Kit K400 100 Assays F

Phospholipase D (PLD) Activity Assay Kit K725 100 Assays C

Phospholipid Assay Kit K351 100 Assays C/F

PicoProbe™ Phosphatidic Acid Assay Kit K748 100 Assays F

PLTP Activity Assay Kit II K593 100 Assays F

PLTP Inhibitor Drug Screening Kit K605 100 Assays F

PUFA

s

PPARγ Ligand Screening/Characterization Assay Kit K437 100 Assays F

Seri

ne

EZClick™ O-GalNAc Modified Glycoprotein Assay Kit (FACS/Microscopy, Green Fluorescence) K560 100 Assays N/A

Phospho-p53 (Ser15) Translocation Assay Kit (Cell-Based) K966 50 Assays N/A

Sora

feni

b

PathwayReady™ EGFR Signaling Inhibitor Panel K863 1 Set N/A

PathwayReady™ MAP Kinase Signaling Inhibitor Panel K862 7 Inhibitors N/A


Ferroptosis


Target Cat. No. Size CAS. No.

Act

ivat

ors

Erastin 2231 1 mg, 5 mg 571203-78-6

Artesunate B2332 50 mg, 250 mg 88495-63-0

BAY 43-9006 (Free base) 2142 25 mg, 100 mg 284461-73-0

BAY 43-9006 (Tosylate) 1594 25 mg, 100 mg 475207-59-1

EZSolution™ BAY 43-9006 (Tosylate) 2031 25 mg 475207-59-1

EZSolution™ Erastin B1502 1 mg 571203-78-6

FIN56 B2308 5 mg, 25 mg 1083162-61-1

L-Buthionine-(S,R)-sulfoximine B2305 10 mg, 50 mg 83730-53-4

ML-162 B2304 1 mg, 5 mg 1035072-16-2

N-Acetyl-4-benzoquinone Imine B2306 1 mg, 5 mg 50700-49-7

RSL3 B2303 5 mg, 25 mg 1219810-16-8

Simvastatin 1693 50 mg, 250 mg 79902-63-9

Sulfasalazine B2331 1 g, 5 g 599-79-1

Inhi

bito

rs

Baicalein B2309 50 mg, 250 mg 491-67-8

Cilopirox olamine B2336 100 mg, 500 mg 41621-49-2

Coenzyme Q10 B2310 10 mg, 50 mg 303-98-0

Cycloheximide 1041 1 ml, 1 g, 5 g 66-81-9

Deferoxamine mesylate 1883 500 mg, 1 g 138-14-7

Diphenyleneiodonium chloride 2358 5 mg 4673-26-1

Ebselen 2169 5 mg, 25 mg 60940-34-3

EZSolution™ SP-600125 9541 5 mg 129-56-6

Ferrostatin -1 2230 5 mg, 25 mg 347174-05-4

GKT137831 9444 5 mg, 25 mg 1218942-37-0

Idebenone 2311 250 µg, 1 mg 82-89-3

Linagliptin 2240 50 mg, 250 mg 668270-12-0

Liproxstatin-1 B2312 5 mg, 25 mg 950455-15-9

N-Acetyl-L-cysteine B1813 1 g, 5 g 616-91-1

PD-146176 B2313 5 mg, 25 mg 4079-26-9

SB202190 2493 5 mg, 25 mg 152121-30-7

SP600125 1669 5 mg 129-56-6

SU-6656 B2334 5 mg, 25 mg 330161-87-0

Trolox 2076 50 mg, 250 mg 53188-07-1

U0126 1668 5 mg 109511-58-2

Vildagliptin 2188 10 mg, 50 mg 274901-16-5

Zileuton B2314 5 mg, 25 mg 111406-87-2

FerroptosisFerroptosis Biochemicals

Other Glutathione-Related ProductsTarget Cat. No. Size CAS. No.

Prot

eins

Active GST Protein 1243 1 mg N/A

Mouse Recombinant GSTMu 1 6348 100 μg N/A

Mouse Recombinant GSTMu 1 6349 100 μg N/A

Schistosoma japonicum Recombinant GST 6351 100 μg N/AN/A: Not Available


FerroptosisTarget Cat. No. Size CAS. No.

Bioc

hem

ical

s Glutathione ethyl ester B2307 10 mg, 50 mg 92614-59-0GST Inhibitor-1 (Cibacron Blue 3G-A, sodium salt) 1555R 1 g 84166-13-2

GST Inhibitor-2 (Ethacrynic acid) 1556 1 g 58-54-8

Oxidized Glutathione (GSSG) 1241 1 g 27025-41-8

Reduced Glutathione (GSH) 1242 1 g 70-18-8

Ferroptosis


Glucose

Figures. A) Glucose concentration in human urine and serum was estimated using K606-100. B) Hexokinase activity in various samples (orchid leaf, MCF-7, Jurkat and HeLa cell) was determined using K769-100. C) Flow Cytometry histograms, using K681-50 showing the inhibition of glucose uptake by phloretin in Jurkat cells (Black: negative control cells; orange: in the presence of phloretin; blue: without phloretin) . D) 1,5-AG uptake in HeLa cells using K684-50: HeLa cells showing the uptake of AGTrackerTM Reagent in the cytoplasm. Cells were stained with AGTrackerTM Reagent for 30 min. and fixed. Image was taken using a fluorescent microscope with a 60X oil objective lens.

Glucose is without a doubt, the most important sugar in mammalian metabolism. Glucose serves as a vital energy source for many organisms, including plants and animals. BioVision offers the most complete series of assays kits aimed to measure this sugar, metabolites and other enzymes involved in its metabolism.

Glucose


Key Features:

• Non-radioactive, homogeneous assays• Specific assays• Convenient: minimal sample preparation; fast protocols (1-2 hours )• Cost effective: 50/100 assays; High Throughput Screening compatible• Validated: using mammalian tissues, cells, biological fluids

Assay Kits

Target Cat. No. Detection* Detection Limit Sample Type

Met

abol

ite

Glucose K686 C 1000 pmol Serum, cells, tissues, food

Glucose K606 C/F 100 pmol Serum, cells, tissues, urine

Glucose K688 F 10 pmol Serum, cells, tissues, food

Glucose (384-well) K950 C 500 pmol Serum

Glucose/Maltose K618 C/F 500 pmol Serum, cells, tissues

Glucose/Sucrose K616 C/F 1000 pmol Cells, tissues, food

Glucose-1-Phosphate K697 C 1000 pmol Serum, cells, tissues, food

Glucose-6-Phosphate K657 C 1000 pmol Serum, cells, tissues

Glucose-6-Phosphate K687 F 5 pmol Serum, cells, tissues

Enzy

me

Glucose Dehydrogenase K786 C 0.01 mU Cells, tissues, PP

Glucose Oxidase K788 C/F 0.01 mU Cells, tissues, PP

Glucose-6-Phosphate Dehydrogenase K757 C 0.04 mU Cells, tissues, PP

Glucose-6-Phosphate Dehydrogenase K751 F 1 µU Cells, tissues, PP

Hexokinase K769 F 2 µU Serum, cells, tissues, PP

Phosphoglucomutase K774 C 1 mU Cells, tissues, PP

Phosphoglucomutase K770 F 20 µU Serum, cells, tissues, PP

Phosphoglucose Isomerase K775 C 0.1 mU Serum, cells, tissues

Cell

Bead

1,5-Anhydroglucitol Uptake** K684 F N/A Adherent/Suspension

2-NBDG Glucose Uptake** K682 F N/A Adherent/Suspension

Glucose Uptake** K681 F N/A Adherent/Suspension

Glucose Uptake K676 C 10 pmol Adherent/Suspension

Glucose Uptake K666 F 1000 pmol Adherent/Suspension

*C: Colorimetric; F: Fluorometric; PP: Protein Preparation** Suitable for Flow Cytometry and Fluorescence Microscopy

Glucose


Glucose Uptake Kits (Cell Based)Diabetes has been recognized as a disorder of glucose intolerance for many decades. Insulin stimulates the glucose uptake into muscle & adipose tissues to maintain the whole-body glucose homeostasis. Among many different methods available for measuring glucose uptake in cells & tissues, 2-deoxyglucose (2-DG) has been widely used because of its structural similarity to glucose. As with glucose, 2-DG can be taken up by glucose transporters, and metabolized to 2-DG-6-phosphate (2-DG6P). 2-DG6P, however, cannot be further metabolized, and thus accumulates in the cells. The accumulated 2-DG6P is, therefore, directly proportional to 2-DG (or glucose) uptake by cells. BioVision is proud to offer the Simplest, Convenient & Non-Radioactive Glucose Uptake Measurement Kits on the market.

Key Features:

• Super-fast Protocol • Highly Sensitive: Detects glucose uptake as low as 10 pmol • High-Throughput Adaptable • Both Fluorometric (Ex/Em = 535/587 nm) & Colorimetric (OD= 412 nm) formats • Does not require correction for extracellular 2-DG • Ample reagents to perform 100 assays in a 96-well plate format

Product Name Cat. No. SizeGlucose Uptake Colorimetric Assay Kit K676 100 Assays

Glucose Uptake Fluorometric Assay Kit K666 100 Assays

Figures. A) K676 Assay Principle: 2-DG6P is oxidized generating NADPH, and determined by an enzymatic recycling amplification reaction. B) K666 Assay Principle: 2-DG6P is enzymatically oxidized and coupled to BioVision’s PicoprobeTM, generating fluorescence in the presence of NADPH. C) 2-DG Uptake in various cell types, including HeLa, Human Adipocytes, and 3T3-L1 cells.

Assay Kits


Glutamine Metabolism

Key Features:

• Related to sepsis or burns• Required to IL-1 induction by macrophages in response to LPS stimulation• Important for generation of NO via Arginine — Gln has cytotoxic and antimicrobial properties• Gln is fluxed into TCA cycle and hexosamine p.• Gln promotes M2 macrophage polarization • T cell and B cell are Gln regulated• Gln levels affect mTORC1 activity as well

Target Cat. No. SizeGlutamine Colorimetric Assay Kit K556 100 Assays

Nitrite Assay Kit (Griess Reagent) K544 200 Assays, 500 Assays, 1000 Assays

Nitric Oxide Colorimetric Assay Kit K262 200 Assays

Nitric Oxide Fluorometric Assay Kit K252 200 Assays

Nitric Oxide Cell-Based HTS Assay Kit K979 100 Assays

Figure: Glutamine Standard Curve (a). Measurement of Glutamine concentration in human urine (5 µl) (b) & mouse tissues (brain [15 µg], liver [50 µg]) (c). Samples were deproteinized using 10K Spin Column (Cat # 1997) & spiked with known amount of Glutamine (6nmol). Assay was performed following the kit protocol.

Assay Kits


Glycolysis


Key Features:

• Non-radioactive, homogeneous assays• Specific assays• Convenient: minimal sample preparation; fast protocols (1-2 hours )• Cost effective: 100 assays; High Throughput Screening compatible• Validated: using mammalian tissues, cells, biological fluids

Assay KitsTarget Cat. No. Dectection* Dectection Limit Sample Type

Met

abol

ites

Dihydroxyacetone Phosphate K673 F 50 pmol Cells, tissues

Glucose K606 C/F 100 pmol Serum, cells, tissues, urine

Glucose K686 C 1000 pmol Serum, cells, tissues, food

Glucose K688 F 10 pmol Serum, cells, tissues, food

Glucose-6-phosphate K657 C 1000 pmol Serum, cells, tissues

Glucose-6-phosphate K687 F 5 pmol Serum, cells, tissues

2-Phosphoglycerate K778 C/F 20 pmol Cells, tissues

Phosphoenol Pyruvate K365 C/F 100 pmol Cells, tissues

Pyruvate K609 C/F 100 pmol Cells, tissues

Enzy

mes

Aldolase K665 C 100 mU Cells, tissues

Enolase K691 C/F 0.04 mU Cells, tissues

GADPH K680 C 10 mU Cells, tissues

Hexokinase K769 F 2 µU Serum, cells, tissues

Hexokinase K713 C - Potential Inhibitors

Phosphofructokinase K776 C 1 mU Cells, tissues

Phosphoglucose isomerase K775 C 0.1 mU Serum, cells, tissues

Pyruvate kinase K709 C/F 1000 pmol Cells, tissues

Triose Phosphate Isomerase K670 C 4 mU Cells, tissues

Coen

zym

es &

co

fact

ors

ADP K356 C 1000 pmol Tissue, cells, PP

ADP K355 C/F 100 pmol Tissue, cells, PP

ADP/ATP Ratio K255 L < 10 pmol Cells, PP

ATP K354 C/F 100 pmol Tissue, cells, PP

NADH K338 F < 0.5 pmol Tissue, cells, PP

NAD/NADH Ratio K337 C 10 pmol Tissue, cells, serum, urine

NADP/NADPH Ratio K347 C 10 pmol Tissue, cells, PP*C: Colorimetric; F: Fluorometric; L: Luminometer; PP: Protein Preparation

Figure: (a) Dihydroxyacetone Phosphate Standard Curve, (b) Measurement of Dihydroxyacetone Phosphate level in Jurkat (250 µg), and MCF-7 (150 µg) cell lysate and in rat liver (50 µg) and muscle (150 µg) lysate.

Glycolysis


Krebs Cycle Assay KitsThe Krebs Cycle, also known as Tricarboxylic Acid (TCA), is considered one of the central pathway in cellular metabolism. It oxidizes several metabolites containing acetyl groups. The list includes amino acids, carbohydrates and fatty acids. BioVision offers the most complete series of assays measuring various metabolites, cofactors, and enzymes involved in the glycolytic pathway.


Assay Kits

Product Name Cat. No. Detection* Detection Limit Sample Type

Met

abol

ite

Acetyl-CoA† K317 F 0.01 pmol Cells, tissues

Citrate K655 C/F 0.25 nmol Cells, tissues

Fumarate K633 C 2.5 nmol Cells, tissues

Isocitrate K656 C 2 nmol Cells, tissues

α-Ketoglutarate K677 C/F 0.1 nmol Cells, tissues

Malate K637 C 1 nmol Cells, tissues

Oxaloacetate K659 C 1 nmol Cells, tissues

Succinate K649 C 1 nmol Cells, tissues

Enzy

me

Aconitase K716 C 0.1 mU Tissue, cells, PP

Citrate Synthase K318 C 1 mU Tissue, cells PP

Fumarase K596 C 2.5 mU Tissue, cells, PP

Isocitrate dehydrogenase K756 C 0.01 mU Tissue, cells, PP

α-Ketoglutarate dehydrogenase K678 C 0.1 mU Tissue, cells, PP

Malate Dehydrogenase K654 C 0.5 mU Tissue, cells, PP

Pyruvate Dehydrogenase† K679 C 0.1 mU Tissue, cells, PP

Succinyl-CoA synthetase K597 C 0.1 mU Tissue, cells, PP

Succinate dehydrogenase K660 C 0.1 mU Tissue, cells, PP

Coen

zym

e/O

ther

s

FAD K357 C/F 25 pmol Tissue, cells, PP

NAD K337 C 10 pmol Tissue, cells, serum, urine

NADP/NADPH Ratio K347 C 10 pmol Tissue, cells, PP

Phosphate K420 F 25 pmol Tissue, cells, PP

Phosphate K410 C 500 pmol Tissue, cells, serum, urine

*C: Colorimetric; F: Fluorometric; PP: Protein Preparation; †: Not form part of the Cycle

Key Features:


Figure: NADH Standard Curve (A). Malate Dehydrogenase activity in rat muscle extract (0.8 µg), Jurkat cell lysate (0.6 µg), yeast mitochondria (1.2 µg) & MDH Positive Control (B). Referenced MDH Activity in Jurkat cell lysate, rat muscle extract and yeast mitochondrial lysate (C). Assays were performed following the kit protocol.

Krebs Cycle Assay Kits

(A) (B) (C)


Pentose Phosphate Pathway


C: Colorimetric; F: Fluorometric; NA: Not Available; pmol: picomole

Key Features:


Assay Kits


Met

abol

ite Fructose-6-Phosphate K689 C 10 pmo Tissue/Cell Lysate

Glucose-6-Phosphate K657 C 1000 pmol Tissue/Cell Lysate

Glucose-6-Phosphate K687 F 5 pmol Tissue/Cell Lysate

6-Phosphogluconate K217 C 1000 pmol Tissue/Cell Lysate

Enzy

me

Glucose-6-Phosphate Dehydrogenase K751 F 1 µU Serum, Tissues

Glucose-6-Phosphate Dehydrogenase K757 C 0.04 mU Serum, Tissues

Glucose-6-Phosphate Dehydrogenase K225 N/A - Inhibitors

6-Phosphogluconolactonase K540 C 0.04 mU Tissue/Cell Lysate

Coen

zym

e/Io

ns

NADP/NADPH K347 C 10 pmol Tissue/Cell Lysate

NAPDH K349 F 1 pmol Tissue/Cell Lysate

Pentose Phosphate Pathway, or hexose monophosphate shunt, is an alternative metabolic pathway that oxidizes glucose, and generates important building blocks such as NADP, and Ribose-5-phosphate. This biochemical pathway occurs exclusively in the cytoplasm, and can be divided into the oxidative (first three reactions), and non-oxidative stages (last five reactions). BioVision offers one of the most complete series of assays measuring various metabolites, cofactors, enzymes involved in this pathway.

(A) (B)

Figure: 6-Phosphogluconate standard curve, n=3 (A). Measurement of 6-Phosphogluconate in the lysates from Rat Liver (160 µg), Rat Kidney (120 µg), and Rat Heart (60 µg). (B). Assays were performed following kit protocol.

Pentose Phosphate Pathway


Tryptophan Metabolism

Key Features:

• Trp ↑ : phenotype characterized by aberrant eosinophil• IDO (Indoleamine-2,3-dioxygenase). Limiting step

— LPS stimulates IDO — IFNγ stimulates TRP metabolism via IDO

• T cells require TRP in vitro (cell proliferation)• No TRP: accumulation of charged tRNAs

Target Cat. No. SizeIndoleamine 2,3-Dioxygenase 1 (IDO1) Activity Assay Kit K972 100 Assays

Indoleamine 2,3-Dioxygenase 1 (IDO1) Inhibitor Screening Kit K976 100 Assays

Tryptophan Assay Kit (Fluorometric) K557 100 Assays

Figure: (a) Measurement of IDO1 inhibition in presence and absence of 1 μM of IDO5L (a potent, highly selective competitive IDO1 inhibitor) and 100 μM of norharmane (a natural product that acts as a weak IDO1 inhibitor). The no inhibitor reaction contained assay buffer with 0.1% DMSO (v/v) as a solvent control. (b) Dose-response curve for IDO1 inhibition by the included selective IDO1 Inhibitor IDO5L. The IC50 value (24.2 ± 1.3 nM) was derived by 4-parameter logistic curve fitting with each point representing the mean ± SEM of at least 3 replicates. All assays were performed according to the kit protocol.

Assay Kits


Tryptophan Metabolism Urea CycleMammals excrete excess nitrogen via the Urea cycle: urea is synthesized in liver, secreted into the bloodstream, delivered to the kidneys were it is finally excreted in urine. The Urea cycle involves five enzymatic reactions, the first two steps are mitochondrial and the last three reactions are cytosolic. BioVision offers one of the most complete series of assays measuring various metabolites, cofactors, enzymes involved in this pathway.


Key Features:



Met

abol

ite

α-ketoglutarate* K677 C/F 0.1 nmol Cell/Tissue lysate

Ammonia K370 C 1 nmol Urine, Cell Lysate

Ammonia K470 C 0.5 nmol Cell/Tissue lysate

Arginine K749 C 5 nmol Serum, beverages

Aspartate K552 C/F 0.1 nmol Serum, tissue lysate

Fumarate K633 C 1 nmol Cell/Tissue lysate

Glutamate* K629 C 1 nmol Serum, foodstuff

Malate* K637 C 1 nmol Serum, lysates

Oxaloacetate* K659 C/F 1 nmol Serum, lysates

Phosphate K420 F 0.1 nmol Serum, lysates

Phosphate K410 C 0.5 nmol Serum, urine

Urea K375 C 0.5 nmol Serum, urine

Urea K376 C 10 nmol Urine, serum, lysates

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me

Alanine Aminotransferase (ALT)* K752 C/F 10 mU Serum, lysates

Arginase K755 C 1 mU Cell/Tissue lysate

Arginase (Inhibitor Screening) K567 C NA Inhibitors

Asparate Aminotransferase* K753 C 10 mU Serum, lysates

Fumarase* K596 C 0.1 mU Cell/Tissue lysate

Glutamate Dehydrogenase* K729 C 0.01 mU Cell/Tissue lysate

Malate Dehydrogenase* K654 C 0.5 mU Cell/Tissue lysate

Malate Dehydrogenase* K538 F 0.04 mU Cell/Tissue lysate

Tyrosine Aminotransferase* K486 F 4 µU Cell/tissue lysates

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ors

ADP K356 C 1000 pmol Tissue, cells, PP

ADP K355 C/F 100 pmol Tissue, cells, PP

ATP K354 C/F 100 pmol Tissue, cells, PP

ADP/ATP Ratio K255 L < 10 pmol Cells, PP

NADH K338 F < 0.5 pmol Tissue, cells, PP

NAD/NADH Ratio K337 C 10 pmol Tissue, cells, serum, urine

NADP/NADPH Ratio K347 C 10 pmol Tissue, cells, PP*Not a part of the Urea Cycle; C: Colorimetric; F: Fluorometric; PP: Protein Preparation; NA: Not Available

Figure: Estimations of L-arginine in two human serum sample (30 µl) and orange juice (1.6 µl). L-arginine concentrations were 0.278 mM and 0.069 mM in the two human serum samples, and 5.113 mM in orange juice. Assays were performed following the kit protocol.

Urea Cycle

Assay Kits


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