team project chemicals- tryptophan
DESCRIPTION
Team Project Chemicals- Tryptophan. 組員： 林瑋耿 , 吳東耘 , 許博凱 , 彭嘉冠. Tryptophan. Molecular formula C 11 H 12 N 2 O 2 Molecular weight 204.23 Isoelectric point pH 5.89 CAS number 73-22-3. Functional group : indole. Where to get it ?. Tryptophan was available in health food - PowerPoint PPT PresentationTRANSCRIPT
System Biology
Team ProjectChemicals- Tryptophan
組員：林瑋耿 , 吳東耘 , 許博凱 , 彭
嘉冠
System Biology
Tryptophan
• Molecular formula– C11H12N2O2
• Molecular weight– 204.23
• Isoelectric point– pH 5.89
• CAS number– 73-22-3
Functional group : indole
System Biology
Where to get it ?
• Tryptophan was available in health food– Banana, milk, fish, and etc.
…
System Biology
What are its usage ?
• Tryptophan is effective sleep aid.
• It shows considerable promise as an antidepressant.
• Other promising indications include reduce some emotional disorders.– Impulsive, violent, and etc. disorders
System Biology
Cont.
• 人類必需胺基酸之一種 ( 無法自行合成 )• 合成 serotonin 的原料 , 是神經傳導物質之一種 ,
能抑制食慾產生飽足感• 合成 melatonin 的原料 , 能幫助睡眠 , 安定心情 ,
增強免疫力 , 常被用來調整時差• Serotonin 和 melatonin 的量會隨著日夜而波動 ,
推測與生理時鐘的調節有關• 維生素 B3 ( 菸鹼酸 , niacin) 的原料 , 作為輔脢
(coenzyme) 幫助代謝作用進行• 植物生長激素 IAA (auxin) 的原料
System Biology
System Biology
System Biology
NAD+ & NADH
2e- + 2H+
System Biology
Tryptophan Biosynthesis
System Biology
Inhibited by L-tyrosine
Inhibited by L-tryptophan
Inhibited byL-phenyalanine
1
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1- Phospho-2-dehydro-3-deoxyheptonate aldolase [EC:2.5.1.54]2- 3-dehydroquinate synthase [EC:4.2.3.4]3- 3-dehydroquinate dehydratase (3-dehydroquinase) [EC:4.2.1.10] Enolase [EC:4.2.1.11]4- shikimate 5-dehydrogenase [EC:1.1.1.25]5- shikimate kinase II (SKII) [EC:2.7.1.71]6- 3-phosphoshikimate 1-carboxyvinyltransferase [EC:2.5.1.19]7- chorismate synthase [EC:4.2.3.5]
System Biology
Inhibited by L-tryptophan
8 & 9- anthranilate phosphoribosyltransferase / anthranilate synthase component II [EC:2.4.2.18, 4.1.3.27]10 & 11- indole-3-glycerol phosphate synthase (IGPS) / N-(5'-phospho-ribosyl)anthranilate isomerase (PRAI) [EC:4.1.1.48, 5.3.1.24]12- tryptophan synthase [EC:4.2.1.20]
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12
System Biology
System Biology
Tryptophan operon in E.coli
trpD + trpE = enzyme 8 & 9
trpC = enzyme 10 & 11
trpA + trpB = enzyme 12
Control the 5 reactions from chorismate to tryptophan !!
System Biology
Trp operon mechanism
System Biology
References
• KEGG: http://www.genome.jp/kegg/
• EcoCyc: http://www.ecocyc.com/
• free encyclopedia: http://en.wikipedia.org/wiki/Main_Page
System Biology
The End