target exon 1 atg

6

Plasmid ID Gene targeted Talen Sequence (Left talen/spacer/right talen) binding sequences tag Method Segment I. Segment II. Segment III. Segment IV. SD_14 SORL1_ATG-Left Set1 T GCAGTAGCGTTCGCC cgaacatggcgacac GGAGCAGCAGGAGGG A GCAG TAGC GTTC GCC GFP Musunuru- Cowan SD_15 SORL1_ATG-right Set1 T GCAGTAGCGTTCGCC cgaacatggcgacac GGAGCAGCAGGAGGG A CCCT CCTG CTGC TCC RFP Musunuru- Cowan target Exon 1 ATG

Upload: others

Post on 05-Dec-2021

5 views

Category:

Documents

0 download

Report

Download

Embed Size (px):

TRANSCRIPT

Page 1: target Exon 1 ATG

Plasmid ID Gene targeted Talen Sequence (Left talen/spacer/right talen)

binding sequences

tag MethodSegment I. Segment II. Segment III. Segment IV.

SD_14 SORL1_ATG-Left Set1 T GCAGTAGCGTTCGCC cgaacatggcgacac GGAGCAGCAGGAGGG A GCAG TAGC GTTC GCC GFP Musunuru-

Cowan

SD_15 SORL1_ATG-right Set1 T GCAGTAGCGTTCGCC cgaacatggcgacac GGAGCAGCAGGAGGG A CCCT CCTG CTGC TCC RFP Musunuru-

Cowan

target Exon 1 ATG

Page 2: target Exon 1 ATG

Page 3: target Exon 1 ATG

parental GFP control

TALEN SORL1

Page 4: target Exon 1 ATG

16 15 14 13 12 11 10 9 8 7 6 5 4 3 2 1 17 18 19

WT homKO

het KO

het KO

Primer for screening

TALsorlafw: AGAAAGTGCGCGAAAGGGAC 230 bp vor ATG

TALsorlarev: TTGCAAAACTGCTCACCTGTC 301 bp nach ATG

Produkt: 531 bp

Page 5: target Exon 1 ATG

clone 17 – ATG KO

clone 6 – ATG WT

Page 6: target Exon 1 ATG

250

50

SORLA

α-tubulin

ATG - Pipelines

CYP2D6 Variation v1 - PharmVarintron 1, exon 2, intron 2-exon 3, intron 4, exon 5, exon 7-intron 8, intron 7, exon 9 none Panserat et al. 1995 Gaedigk et al. 2010 Gaedigk et al, 2010

CRISPR/Cas9-mediated genome editing induces exon skipping ... · HeLa cells can cause skipping of exon 3, exon 4, or exons 3, 4, and 5 [18]. We also detected infrequent exon skipping

Exon Mobil Interest Netting

PowerPoint Presentation · Retinoblastoma WT QRX-421 Exon 12 Exon 13 Exon 14 Exon 12 Exon 14 In-frame Exon 11 Exon 14 Out of-frame No AON QRX-421 Exon 12 Exon 13 Exon 14 Erwin van

Exon Autumn 2010

4: Genome evolution. Exon Shuffling 3 types of exon shuffling exon duplication = the duplication of one or more exons within a gene (internal duplication)

Ryan Exon Portfolio

ClinicalStudy - Hindawi Publishing Corporationdownloads.hindawi.com/archive/2017/8196434.pdf · ChemotherapyResearchandPractice 3 Exon 19-censored Exon 21-censored Exon 19 Exon 21

ATG - SEO In ATG

AUTOMATED TEST GENERATION Muhammed İ. KALKAN. Automatic Test Generation (ATG) Index Index What is ATG? What is ATG? ATG Types ATG Types Why do we need

Desastre Exon Valdez

Exon Mobile Drilling Guide

ΠΑΡΟΥΣΙΑΣΗ ΠΕΡΙΣΤΑΤΙΚΟΥ · p.Met13Ile (p.Met33Ile) c.99G>C ATG>ATC Exon 2 non-amyloidogenic German Altland (1999) The 4th International Symposium on FAP and

Sources of off-target expression from recombinase ... · Sources of off-target expression from recombinase-dependent AAV vectors and mitigation with cross-over insensitive ATG-out

UvA-DARE (Digital Academic Repository) Molecular and genetic … · exon 6 [-21M13]- ACA ggA ACT gTC ATT gTC ATg [M13-Rev]- CAg gAA CgA AgA gAC CTA Ag exon 7 and 8 [-21M13]- Tgg AAg

Exon Mobil Spill 2011

DEFECTIVE(EXON(RECOGNITION( …library/doktorati/genetika/4Alanis.pdf · 2011. 10. 26. · 2.3.2.*Effect*of*modified*U1*snRNAs*on*FIX*exon*5*splicing**61 Section*2.4.!Effect!of!Exon!Specific!U1!snRNAs!(ExSpeU1s)!on!CFTR!exon!

EXON - exeter.ox.ac.uk

Exon selection factor

oracle atg training | oracle atg training courses | oracle atg commerce training

Exon-Mobile Drilling Guide

Chapter 15mmsalemscienceteacher.weebly.com/uploads/2/3/3/6/... · 2018. 9. 9. · Exon 1 Intron Exon 2 Branch point A snRNA Exon 1 Exon 2 Lariat 5′ 5′ 3′ 3′ 5′ 5′ 3′

presentacion atg

Exon Mobil Mock Case

YNYUR A Y (YYYYY) · (YYYYY) n Branch Point Polypyrimidine Tract ~40 nts YNYUR A Y Intron Exon A B Figure 1 . Intron de˜nition Exon de˜nition Splice-site recognition Exon A Exon