t5000 and plex id overview - nfstc projects · plex ‐ id ™ system features and benefits •...
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TechnologyTransition Workshop
Ibis™ T5000™ and PLEX‐ID™
Hardware Overview
Technology Transition Workshop| Kristin S. Lowery, Ph.D.
TechnologyTransition Workshop
Ibis™ T5000™ Components
• Bruker Daltonics micrOTOF™
• Automated heat sealer• Clean‐up module• Thermo Electron robotic arm• LEAP Technologies autosampler• Ibis™ T5000™ software
• Ibis™ T5000™ processor• Processing computer• Data acquisition computer• JUN‐AIR® compressor (optional)
Forensic SNP Analysis 2Ibis™ T5000™ and PLEX‐ID™ Hardware Overview
TechnologyTransition Workshop
Loading Ibis™ T5000™ – PlatesHotel 2Magnetic bead plate
Barcodes must face the arm
Hotel 3Elution (ESI) plate
Barcode reading station
Hotel 1Crude PCR plate
Forensic SNP Analysis 3Ibis™ T5000™ and PLEX‐ID™ Hardware Overview
TechnologyTransition Workshop
Loading Ibis™ T5000™ – Reagents
• Reagent consumption about 50 ml per plate
• System liquid about 150 ml per plate
• System waste about 300 ml per plate (25 to 30% MeOH)
Clean‐up reagent 1, 2 and 3 bottles
System water (front)
System waste (back)
Clean‐up reagent 3
System waste
Forensic SNP Analysis 4Ibis™ T5000™ and PLEX‐ID™ Hardware Overview
TechnologyTransition Workshop
Ibis™ T5000™ – In Action(Video T5000_0002.wmv)
Forensic SNP Analysis 5Ibis™ T5000™ and PLEX‐ID™ Hardware Overview
TechnologyTransition Workshop
PLEX‐ID™ System
Forensic SNP Analysis 6Ibis™ T5000™ and PLEX‐ID™ Hardware Overview
© 2009 Abbott
TechnologyTransition Workshop
PLEX‐ID™ System
Forensic SNP Analysis 7
© 2009 Abbott
Ibis™ T5000™ and PLEX‐ID™ Hardware Overview
TechnologyTransition Workshop
PLEX‐ID™ System
Forensic SNP Analysis Ibis™ T5000™ and PLEX‐ID™ Hardware Overview 8
© 2009 Abbott
TechnologyTransition Workshop
PLEX‐ID™ System – In Action(Video PLEXIDVIDEO_0001.wmv)
Forensic SNP Analysis 9Ibis™ T5000™ and PLEX‐ID™ Hardware Overview
TechnologyTransition Workshop
PLEX‐ID™ System Features and Benefits
• Expedites delivery of results with the ability to process 300 samples in a 24 hour period
• Offers the efficiency of 15 tray load capability− 6 or 12 samples / tray
• Has STAT capabilities
Forensic SNP Analysis 10Ibis™ T5000™ and PLEX‐ID™ Hardware Overview
TechnologyTransition Workshop
Questions?
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TechnologyTransition Workshop
Contact Information
Kristin S. Lowery, Ph. D.
Ibis Biosciences, a subsidiary of Abbott Molecular
Forensic SNP Analysis 12Ibis™ T5000™ and PLEX‐ID™ Hardware Overview