t. · lck lck2/ick nek2 nek3 nek4 nek5 nek6 nek7 nek9 nek11 nim1 nlk osr1/oxsr1 p38a/mapk14...

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Whats Inside?

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Kinase Assays Wild Type....................................2-4 Mutant...........................................5 Atypical.........................................6 Lipid...............................................6 Full Panel.......................................6

Methyltransferase Assays Including Histone Methyltransferases and DNA Methyltransferases

Reader Domain Assays Bromodomain Chromodomain PHD Domain SRA Domain Tudor Domain Multiple Domain

Histone Deacetylase (HDAC) AssaysSirtuin (SIRT) Assays Histone Acetyltransferase (HAT) AssaysDemethylase AssaysDeubiquitinase (DUB) Assays PARP Assays Isocitrate Dehydrogenase (IDH) Assays Protease Assays Cytochrome P450s (CYP) AssaysPhosphodiesterase (PDE) AssaysHeat Shock Protein 90 (HSP90) AssayshERG AssaysB-cell Lymphoma (BCL) AssaysPhosphatase AssaysNew Assays Cell AssaysThermal Shift AssaysIsothermal Titration Calorimetry (ITC) AssaysSurface Plasmon Resonance (SPR) Assays

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Reaction Biology feels like a family company to me but is a big company in a good way. I feel like a person, a client and am treated 1st class. However, I get results that are of the highest professional standard, worthy of a bigger corporation. I feel that big companies treat you as a “number”, churn out data and forget about you once the invoice has been settled. With you, it’s like I know you all personally and you always have time to respond to queries and are very friendly

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Over 1000 Assays For Drug Discovery Research

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g

Kinase HotSpot600+ Kinases

Wild Type Kinases (369)

CAMK2bCAMK2dCAMK2g CAMK4CAMKK1CAMKK2CDC7/DBF4CDK1/cyclin ACDK1/cyclin BCDK1/cyclin ECDK2/cyclin A1CDK2/cyclin ACDK2/cyclin ECDK2/cyclin OCDK3/cyclin ECDK4/cyclin D1CDK4/cyclin D3CDK5/p25CDK5/p35CDK6/cyclin D1CDK6/cyclin D3CDK7/cyclin HCDK9/cyclin KCDK9/cyclin T1CDK9/cyclin T2CDK14 (PFTK1)/cyclin YCDK16/cyclin YCDK17/cyclin Y (PCTK2)CDK18/cyclin YCDK19/cyclin C CHK1 CHK2CK1a1CK1a1L

CK1d CK1epsilonCK1g1CK1g2CK1g3CK2aCK2a2c-KitCLK1CLK2CLK3CLK4c-MERc-METCOT1/MAP3K8CSKc-SrcCTK/MATKDAPK1DAPK2DCAMKL1DCAMKL2DDR1DDR2DLK/MAP3K12DMPKDMPK2/CDC42BPGDRAK1/STK17ADYRK1/DYRK1ADYRK1BDYRK2DYRK3DYRK4EGFR

ABL1ABL2/ARGACK1AKT1AKT2AKT3ALKALK1/ACVRL1ALK2/ACVR1ALK3/BMPR1AALK4/ACVR1BALK5/TGFBR1ALK6/BMPR1BARAFARK5/NUAK1ASK1/MAP3K5Aurora AAurora BAurora CAXLBLKBMPR2BMX/ETKBRAFBRKBRSK1BRSK2BTKCAMK1aCAMK1bCAMK1dCAMK1gCAMK2a

Kinase


Gold Standard Radiolabeled Data.Avoid false postives & negatives caused by other assay formats.

608 Kinases & Growing.The largest panel of activity based kinase assays.

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Stringent Control System.One free control compound included in every kinase assay and in your report.

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Choose One of our Bi-monthly Full Panels:

369 Wild Type Kinase Panel202 Mutant Kinase Panel20 Atypical Kinase Panel

17 Lipid Kinase Panel

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Wild Type Kinases (continued)LIMK1 LIMK2LKB1LOK/STK10LRRK2LYNLYN BMAKMAPKAPK2MAPKAPK3MAPKAPK5/PRAKMARK1MARK2/PAR-1BaMARK3MARK4MEK1MEK2MEK3 MEK5MEKK1MEKK2MEKK3MEKK6MELKMINK/MINK1MKK4MKK6MKK7MLCK/MYLKMLCK2/MYLK2MLK1/MAP3K9MLK2/MAP3K10MLK3/MAP3K11MLK4MNK1MNK2MRCKa/CDC42BPAMRCKb/CDC42BPB

EPHA1EPHA2EPHA3EPHA4EPHA5EPHA6EPHA7EPHA8EPHB1EPHB2EPHB3EPHB4ERBB2/HER2ERBB4/HER4ERK1/MAPK3ERK2/MAPK1ERK5/MAPK7ERK7/MAPK15ERN1/IRE1ERN2/IRE2FAK/PTK2FERFES/FPSFGFR1FGFR2FGFR3FGFR4FGRFLT1/VEGFR1FLT3FLT4/VEGFR3FMSFRK/PTK5FYNGCK/MAP4K2GLK/MAP4K3GRK1GRK2

Kinase


GRK3GRK4 GRK5GRK6GRK7GSK3aGSK3bHaspinHCKHGK/MAP4K4HIPK1HIPK2HIPK3HIPK4HPK1/MAP4K1IGF1RIKKa/CHUKIKKb/IKBKBIKKe/IKBKEIRIRAK1IRAK4IRR/INSRRITKJAK1JAK2JAK3JNK1JNK2JNK3KDR/VEGFR2KHS/MAP4K5KSR1KSR2LATS1LATS2LCKLCK2/ICK

NEK2NEK3NEK4NEK5NEK6NEK7NEK9NEK11NIM1NLKOSR1/OXSR1P38a/MAPK14P38b/MAPK11P38d/MAPK13P38g/MAPK12p70S6K/RPS6KB1p70S6Kb/RPS6KB2PAK1PAK2PAK3PAK4PAK5PAK6PASKPBK/TOPKPDGFRaPDGFRb

CAMK4CAMKK1CAMKK2CDC7/DBF4CDK1/cyclin ACDK1/cyclin BCDK1/cyclin ECDK2/cyclin A1

MSK1/RPS6KA5 MSK2/RPS6KA4MSSK1/STK23MST1/STK4MST2/STK3MST3/STK24MST4MUSKMYLK3MYLK4MYO3AMYO3BNEK1NEK2NEK3NEK4NEK5NEK6NEK7NEK8NEK9NEK11NIM1NLKOSR1/OXSR1P38a/MAPK14P38b/MAPK11P38d/MAPK13P38g/MAPK12p70S6K/RPS6KB1p70S6Kb/RPS6KB2PAK1PAK2PAK3PAK4PAK5PAK6PASK

PKCaPKCb1PKCb2PKCdPKCepsilonPKCetaPKCgPKCiotaPKCmu/PRKD1PKCnu/PRKD3

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Wild Type Kinases (continued)

STK33STK38/NDR1STK38L/NDR2STK39/STLK3SYKTAK1TAOK1TAOK2TAOK3/JIKTBK1TECTESK1TESK2TGFBR2TIE2/TEKTLK1TLK2TNIK TNK1TRKATRKBTRKCTSSK2TSSK3/STK22CTTBK1 TTBK2TXKTYK1/LTKTYK2TYRO3/SKYULK1ULK2ULK3VRK1VRK2WEE1

Kinase


WNK1WNK2 WNK3YES/YES1YSK4/MAP3K19ZAK/MLTKZAP70ZIPK/DAPK3

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PBK/TOPKPDGFRa PDGFRbPDK1/PDPK1PEAK1PHKg1PHKg2PIM1PIM2PIM3PKAPKAcbPKAcgPKCaPKCb1PKCb2PKCdPKCepsilonPKCetaPKCgPKCiotaPKCmu/PRKD1PKCnu/PRKD3PKCthetaPKCzetaPKD2/PRKD2 PKG1aPKG1bPKG2/PRKG2PKN1/PRK1PKN2/PRK2PKN3/PRK3PLK1PLK2PLK3PLK4/SAK

PRKXPYK2RAF1RETRIPK2RIPK3RIPK4RIPK5ROCK1ROCK2RON/MST1RROS/ROS1RSK1RSK2RSK3RSK4SBK1SGK1SGK2SGK3/SGKLSIK1SIK2SIK3SLK/STK2SNARK/NUAK2SNRKSRMSSRPK1SRPK2SSTK/TSSK6STK16STK21/CITSTK22D/TSSK1STK25/YSK1STK32B/YANK2STK32C/YANK3

GET RESULTS

Reports will include raw data, % enzyme activity or % inhibition, control compound’s IC50 values and IC50 values with curve fitting.

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Mutant Kinases continued (202+)


Kinase

ABL1 (E255K)ABL1 (E255V)ABL1 (F317I)ABL1 (F317L)ABL1 (G250E)ABL1 (H396P)ABL1 (M351T)ABL1 (Q252H)ABL1 (T315I)ABL1 (V299L)ABL1 (Y253F)ABL1 (Y253H)AKT1 (E17K)AKT2 (E17K)AKT3 (E17K)AKT3 (G171R)ALK (C1156Y)ALK (L1152R)ALK (L1196M)ALK (F1174L)ALK (F1174L)-NPM1ALK (F1174S)ALK (G1202R)ALK (G1269A)ALK (G1269S)ALK (R1275Q)ALK (S1206R)ALK (T1151M)ALK (T1151-L1152insT)ALK (F1174L)-EML4ALK-NPM1ALK-TFGALK-TPM3ALK (Q207D)ALK2 (R206H)AXL (R499C)BRAF (d485-489/P490Y)BRAF (G464V)BRAF (G469A)BRAF (K601E)BRAF (L597V)

BRAF (T599_V600insT)BRAF (V599E)BRAF (V600A)BRAF (V600D)BRAF (V600K)BRAF (R506_K507insVLR)BRAF-FAM131B (Fex2Bex9)BRAF-KIAA1549 (Kex15Bex9)BRAF-KIAA1549 (Kex16Bex9)BRAF-SRGAP3 (Sex12Bex9)BTK (C481S)BTK (E41K)BTK (P190K)CHK2 (I157T)c-Kit (A829P)c-Kit (d557-558)c-Kit (D816E)c-Kit (D816F)c-Kit (D816Y)c-Kit (D816H)c-Kit (D816I)c-Kit (D816V)c-Kit (D820E)c-Kit (D820Y)c-Kit (K642E)c-Kit (T670I)c-Kit (V559A)c-Kit (V559D)c-Kit (V559D/V654A)c-Kit (V559D/T670I)c-Kit (V560G)c-Kit (V560G/D816V)c-Kit (V560G/N822K)c-Kit (V654A)c-Kit (Y823D)c-MER (A708S)c-MET (D1228H)c-MET (D1228N)c-MET (F1200I)c-MET (M1250T)c-MET (P991S)

c-MET (T992I)c-MET (T1173I) c-MET (V1092I)c-MET (Y1230A)c-MET (Y1230C)c-MET (Y1230D)c-MET (Y1230H)c-MET (Y1235D)c-Src (T341M)DDR2 (N456S)DDR2 (T654M)EGFR (A763_Y764insFHEA)EGFR (C797A)EGFR (C797S)EGFR (T790M/C797S/L858R)EGFR (d746)EGFR (d746-750/T790M/C797S)EGFR (d747-749)EGFR (D761Y)EGFR (D770GY)EGFR (G719C)EGFR (G719D)EGFR (G719S)EGFR (L747S)EGFR (L858R)EGFR (L861Q)EGFR (T790M)EGFR (T790M, L858R)EGFR (d746-750/T790M)EGFR (d746-750)EGFR (d746-750/C797S)EGFR (d747-749/A750P)EGFR (d747-752/P753S)EGFR (d752-759)EGFR (D770_N771insNPG)ERBB2 (D769H)ERBB2 (D769Y)ERBB2 (P780_Y781insGSP)ERBB2 (V777L)ERBB2 (V777_G778insCG)FGFR1 (V561M)

FGFR1OP-FGFR1FGFR2 (E565G)FGFR2 (K526E)FGFR2 (K641R)FGFR2 (K659N) FGFR2 (N549H)FGFR2 (R612T)FGFR2 (V564F)FGFR3 (V555M) FGFR3 (G697C)FGFR3 (K650E)FGFR3 (K650M)FGFR3 (K650Q)FGFR4 (N535K) FGFR4 (V550E) FGFR4 (V550L)FGFR4 (V550M)FLT3 (D835Y)FLT3 (ITD)FLT3 (ITD)-NPOSFLT3 (ITD)-W51FLT3 (F594_R595insR)FLT3 (F595_R595insREY)FLT3 (R595_E596insEY)FLT3 (Y591_V592insVDFREYEYD)FYN (Y531F)JAK2 (617F)KSR1 (A635F)KSR1 (L639F)KSR2 (R676S)LRRK2 (G2019S)LRRK2 (I2020T)LRRK2 (R1441C)MEK1 (P124L)MELK (T460M)p38a (T106M)PDGFRa (D842V)PDGFRa (T674I)PDGFRa (V561D)PDGFRa-FIP1L1

PDGFRb-TPM3PKD2 (G870E)RET (A883F)RET (E762Q)RET (G691S)RET (L790F) RET (M918T)RET (R749T)RET (R813Q)RET (S891A)RET (S904F)RET (S904A)RET (V778I)RET (V804E)RET (V804L)RET (V804M)RET (Y791F)RET (Y806H) RET-BCRRET-CCDC6 (PTC1)RET-PRKAR1A (PTC2)RET-NCOA4 (PTC3)ROS1 (G2032R)ROS1-GOPCROS1-TPM3RSK2 (I416V)RSK2 (L608F)TIE2 (A1124V)TIE2 (P883A)TIE2 (R849W)TIE2 (Y897C)TIE2 (Y897STIE2 (Y1108F)TRKA (G667C)TRKA-TFG (TRK-T3)TRKA-TPM3TRKA-TPRYES1 (T348I)ZAP70 (Y319F)

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Kinase HotSpot


Large Scale HTS Large Scale Single Dose Duplicate ProfilingIC50 Profiling-5 or 10 Dose with Curve Fitting

ATP Competitive Evaluation Compound Binding Reversibility TestsKi Determination Assay Services Custom Assay Development

Substrate Determination Assay Early Drug Discovery Collaboration

Atypical Kinases (20)

AMPK(A1/B1/G1) AMPK(A1/B1/G2) AMPK(A1/B1/G3) AMPK(A1/B2/G1) AMPK(A2/B1/G1) AMPK(A2/B2/G1)AMPK(A2/B2/G2)AMPK(A2/B2/G3)DNA-PKEEF2K

EIF2AK1/HRIEIF2AK2EIF2AK3/PERKEIF2AK4/GCN2mTOR/FRAP1PDK1/PDHK1 PDK2/PDHK2 PDK3/PDHK3 PDK4/PDHK4TRPM7/CHAK1

PI3K (p110a(E542K)/p85a)PI3K (p110a(E545K)/p85a)PI3K (p110a(H1047R)/p85a)PI3K (p110a/p65a)PI3Ka (p110a/p85a)PI3Kb (p110b/p85a) PI3Kd (p110d/p85a)PI3Kg (p120g)PI3KC2a

PI3KC3PI4K2API4KaPI4KbPIP5K1APIP5K1CSPHK1SPHK2

Lipid Kinases (17)

Kinase

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Our kinase panels are run on a monthly schedule, which allows us to offer very competitive pricing, with a turnaround time of only two weeks!

The Largest Kinase Panel (600+ Kinases)

All Assays Radioisotope Based (with the exception of lipid kinases)

2 Week Turnaround

As you know, we were a little hesitant on such a broad panel at such an early stage in our project, but we feel it was well worth it.

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600+ Kinases

6

gThe Largest Methyltransferase PanelOne Free Control Compound in Every Assay

HMT HotSpot40+ Methyltransferases

Histone Lysine & Histone Arginine Methyltransferases

ASH1LDOT1LEZH1 ComplexEZH2 ComplexEZH2 (Y641F) ComplexG9a GLPMLL1 ComplexMLL2 ComplexMLL3 ComplexMLL4 ComplexNSD1NSD2NSD2 (E1099K)NSD2 (T1150A)NSD3PRDM9PRMT1 PRMT3 PRMT4PRMT5PRMT5/MEP50PRMT6PRMT7PRMT8SET1B ComplexSET7 SET8SETD2SMYD1SMYD2SUV39H1 SUV39H2 SUV420H1-tv2

Epigenetic Assays

1


Target Radioactivity Based

DNMT1 DNMT3a DNMT3b DNMT3b /3L

Protein Available

Choose between Nucleosomes,

Histone Proteins, andPeptides as Substrates

DNA Methyltransferases

Other

Target Radioactivity Based Protein Available

COMT*COMT (V108M)*METTL21ANRMT1NRMT2

*FlashPlate Assay

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HTS & Profiling Alphascreen, Thermal Shift & ITC Assays One Free Control Compound in Every Assay

Reader Domain Assays

Epigenetic Assays


ASH1L-[BRD] ATAD2ATAD2BBAZ1A BAZ1BBAZ2ABAZ2BBPTF-[BRD] BPTF-[PHD-BRD] BRD1BRD2-1BRD2-2BRD2-TandemBRD3-2BRD3-2BRD3-TandemBRD4 Full LengthBRD4-1BRD4-2BRD4-TandemBRD7BRD9BRDT-1BRDT-2BRDT-TandemBRPF1aBRPF1bBRPF3BRWD1-2BRWD3-2CBX7-[CHR] CECR2CDgamma1-[CHR]CHD1-[CHR]CHD2-[CHR]CHD4-[CHR]CHD4-[PHD-CHR]CHD7-[CHR]CREBBPEP300HP1alpha

Reader Domain Proteins (Available with GST or His tag)Thermal Shift AlphaScreen Protein AvailableTarget

Continued on next page >

Melt Curve for BRD4-1 (His) Thermal Shift

Temperature (Celsius)

RFU

(10^

3)

Target BRD4-1 (His)

CompoundJQ1PFI1BET151DMSO

Melt Temp57.553.557.548

Tm9.55.59.50

Log [Compound] (M)

% A

ctiv

ity

Reference Compound IC50 for BRD4 (AlphaScreen)

EVENING DESERT SAFARI

*

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HTS & Profiling Alphascreen, Thermal Shift & ITC Assays AvailableOne Free Control Compound in Every Assay

Reader Domain Assays

Epigenetic Assays


HP1alpha-[CHR] HP1betaHP1beta-[CHR]HP1gamma KAT2AKAT2BKAT5-2-[CHR]KAT5-3-[CHR]L3MBTL1MPP8-[CHR] PB1-1PB1-2PB1-3PB1-4PB1-5PB1-6PHIP-2PHIP-TandemSMARCA2aSMARCA2bSMARCA4SP100SP110CSP140SP140LTAF1-1TAF1-2TAF1L-1TAF1L-2TAF1L TandemTRIM24TRIM28TRIM33aTRIM33bTRIM66UHRF1-[PHD]UHRF1-[SRA]UHRF1-[TDR]UHRF1-[TDR-PHD]UHFR1-FL

Reader Domain Proteins (Available with GST or His tag)

Thermal Shift AlphaScreen Protein AvailableTarget

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Thank you for the beautiful curves! I am very comfortable with this data.“ ”

*No small molecule control is available

**

*

***

*

Reference Compound IC50 for BRDT-1 (AlphaScreen)

g

HTS & ProfilingOne Free Control Compound in Every Assay

HDACs/HAT Assays

HDAC (Histone Deacetylase) Assays:

HDAC1HDAC2HDAC3/NCOR2HDAC4HDAC5HDAC6HDAC7HDAC8HDAC9HDAC10HDAC11HeLa Nuclear extracts

HAT (Histone Acetyltransferase) Assays:

SIRT (Sirtuin) Assays:

SIRT1SIRT2SIRT3SIRT5

P300CBPhGCN5KAT5MYST2MYST4pCAF

Epigenetic Assays


Fluorescence BasedFluorescence BasedFluorescence BasedFluorescence BasedFluorescence BasedFluorescence BasedFluorescence BasedFluorescence BasedFluorescence BasedFluorescence BasedFluorescence BasedFluorescence Based

Target Technology

Fluorescence BasedFluorescence BasedFluorescence BasedFluorescence Based

Protein Also Available

Target Technology Protein Also Available

Target Technology

Radioactivity BasedRadioactivity BasedRadioactivity BasedRadioactivity BasedRadioactivity BasedRadioactivity BasedRadioactivity Based

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I am very happy with the dedication of the professional services provided by Reaction Biology. You guys are awesome!

“”

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Additional Epigenetic Assays

A20Ataxin3BAP1NEDP1SENP1UCHL1UCHL3USP10USP14USP2USP5USP8

PARP Assays

PARP1PARP2PARP5B

Epigenetic Assays

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Target Technology

FluorescentFluorescent Fluorescent FluorescentFluorescentFluorescentFluorescentFluorescentFluorescentFluorescentFluorescentFluorescent

Target Technology

Radioactivity Radioactivity Radioactivity

Isocitrate dehydrogenase (IDH) Assays

IDH1 R132CIDH1 R132HIDH1 WTIDH2 R140QIDH2 R172QIDH2WT

FluorescentFluorescent Fluorescent FluorescentFluorescentFluorescent

Target Technology

Protein Available

Demethylase Assays

KDM4AKDM4DKDM5AKDM5BKDM6BLSD1

FluorescentFluorescent Fluorescent FluorescentFluorescentFluorescent

DUB (Deubiquitinase) Assays

Target Technology

Protein Available

Quinone Oxidoreductase (NQO) Assays

NQO1 Fluorescent

gHTS & Profiling 62 Targets & GrowingOne Free Control Compound in Every Assay

Protease Assays

Proteases

ACE1ACE2ADAM10BACE1Calpain 1Caspase 1Caspase 2Caspase 3Caspase 4Caspase 5Caspase 6Caspase 7Caspase 8Caspase 9Caspase 10Caspase 11Cathepsin BCathepsin CCathepsin GCathepsin HCathepsin LCathepsin SCathepsin VChymaseChymotrypsinDPPIVDPPVIIIDPPIX

Other Assays

Proteinase KTACEThrombin aTrypsinTryptase b2Tryptase g1Urokinase

DPPIXElastaseFVIIaFXaFXIaHIV-1Kallikrein 1Kallikrein 5Kallikrein 7Kallikrein 12Kallikrein 13Kallikrein 14MMP 1MMP 2MMP 3MMP 7MMP 8MMP 9MMP 10MMP 12MMP 13MMP 14Matriptase 2NeprilysinPapainPlasma KallikreinPlasminProteinase A

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gHTS & Profiling

One Free Control Compound in Every Assay

Additional Assays for Early Drug Discovery

Cytochrome P450s (CYPs)

CYP 1A2CYP 2C19CYP 2C9CYP 2D6CYP 3A4

Other Assays

hERG

hERG

B-cell Lymphoma (BCL)

Bcl-2BCL-XLPhosphodiesterases (PDEs)

PDE1APDE2APDE3APDE4APDE4BPDE4CPDE4DPDE5APDE7APDE8APDE9APDE10A

Heat Shock Protein 90 (HSP90)

HSP90aHSP90b

Phosphatase

PP1APP1BPP2A alphaPTPN1_PTP1BPTPN2/TC-PTPPTPN6_SHP1PTPN7_LC-PTPPTPN11_SHP2PTPN12/PTP-PESTPTPRC/CD45DUSP22/MKPX

New Assays

MTH1


Target TechnologyFluorescent basedFluorescent based Fluorescent basedFluorescent basedFluorescent based

Target Technology

Fluorescent basedFluorescent based Fluorescent basedFluorescent based Fluorescent based Fluorescent based Fluorescent basedFluorescent based Fluorescent based Fluorescent based Fluorescent basedFluorescent based

Target Technology

Fluorescent basedFluorescent based

Target TechnologyFluorescent based

Target TechnologyFluorescent basedFluorescent based

Target Technology

Fluorescent basedFluorescent based Fluorescent basedFluorescent based Fluorescent based Fluorescent based Fluorescent basedFluorescent based Fluorescent based Fluorescent based Fluorescent based

Fluorescent based

Target Technology

Inhibitor of Apoptosis Proteins (IAPs)

cIAP-1cIAP-2XIAP

Binding AssayBinding AssayBinding Assay

Target Technology

100+ Cell Lines

Cell Assays

Breast Cancer

Breast Cancer

Liver Cancer

Bone Carcinoma

Ovarian Cancer

Prostate Cancer

Cervical CancerFibrosarcoma

Gastrointestinal Cancer

Head & Neck Cancer

Leukemia

Skin Cancer


Kidney Cancer

Breast Cancer

143BU2OSBT474BT-549 Hs 578BstHs 578TMCF-7MDA-MB-157MDA-MB-231 MDA-MB-361 MDA-MB-468SK-BR-3ZR-75-1HeLaHT-1080DLD-1HCT 116HT-29 MIA PaCa-2Panc-1SNU-16SW480A-253Cal 27DBTRG-05MGDetroit 562IMR-32SCC-25SCC-9T98GU87MGHep G2Hep3BPLC-PRF-5SK-Hep-1SNU-182THLE-3A-549 HCC-78MSTO-211HNCI-H441NCI-H460A-498

Lung Cancer

BV-173CMKH-9HC-1HH HL-60HuT 78HuT 102JurkatK-562KASUMI-2MinoMJ(G11)MOLT-4MV-4-11NK-92NOMO-1RajiREHSUP-B15SU-DHL-1SU-DHL-6SUP-T1SZ-4THP-1KMS-11MM.1SRPMI 8226U266OVCAR-3SK-OV-3DU 145LNCapPC-322Rv1A-375 or A-375-PA-431DU4475FaDuMM127SK-MEL-2TE354.TUACC-62

Myeloma

Normal

CCD1106HDMECHEK-293HGF-1HOKHPLFHUVECMCF 10AMRC-5THLE-3WI-38

Animal

Neuro-2a (N2a)B16-F10CHO-K1MacrophageNIH 3T3NMuMGPC-12RIN-m5FSf9

Other Assays

Cell Assays

Cell Viability & Proliferation

ATPliteCellTiter-Glo® Luminescent

cyQuantMTTMTS

BrdU AssayWST-8 assay

Cell Apoptosis

Apo-ONE® caspase 3/7Mitochondrial Apoptosis Detection

ELISAWestern Blot

AlphaLISAELISA (DELFIA)Western Blot

AlphaLISAELISA (DELFIA)Western Blot

OtherOther Assays


Protein Protein Interactions

NanoBRET Assay

HDAC Inhibitor Testing- Westernblot:• 10uM Tubastatin A (Tu)• 5uM SAHA (S)• 1uM TSA (Ts)• DMSO (D)

15

No chemoattracant Compound A (0 µM)

+ chemoattractant

Compound A (0.1 µM) + chemoattractant




Tumor Invasion Assay

Cell Phosphorylation

Cell Based Histone Methylation

Cell Based Histone Acetylation

Thermal Shift AssaysBiophysical Assays

Test the selectivity of any compound against a wide range of reader domains in our thermal shift assays.


Extensive Coverage of the Reader Domain Family

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Temp, C

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BRD2-1 (his) BRD2-2 (his)

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Isothermal Titration Calorimetry (or ITC) assays provide a complete thermodynamic profile of the interaction of molecules free in solution without modifications, tags, or coupling to a surface.

ITC Assays

With ITC technology, RBC provides:

• Equilibrium binding affinity (Ka)• Binding stoichiometry• Enthalpy of binding ΔH• Entropy (ΔS), free energy of binding (ΔG),

and dissociation constant (Kd) by extension

Biophysical Assays


SPR AssaysBiophysical Assays


SPR Assays (or Surface Plasmon Resonance) measure biomolecular intonations, including small molecules/protein and protein/protein interactions.

Reaction Biology is equipped with the state of the art Biacore 8K instrument with high throughput capability.

With the new Biacore 8K SPR platform, RBC provides:• High throughput fragment library screening• Kineticsandaffinitydetermination• Binding site mapping and competition analysis• Specificitydetermination• Antibody characterization

www.ReactionBiology.comReaction Biology Corporation

1 Great Valley Parkway, Suite 2Malvern, PA 19355

USA

Quality Data. Quality Support.

With the largest functional kinase activity assay panel in the industry, RBC provides ki-nase, epigenetic, and other profiling services to over 700 clients worldwide. RBC special-izes in custom assay conditions, high quality reproducible data, and outstanding service.

“Reaction Biology feels like a family company to me but is a big company in a good way. I feel like a person, a client and am treated 1st class. However, I get results that are of the highest professional standard, worthy of a bigger corporation. I feel that big companies treat you as a “number”, churn out data and forget about you once the invoice has been settled. With you, it’s like I know you all personally and you always have time to respond to queries and are very friendly”

“It is always a pleasure to work with all of you! Your service has always been very reliable (and affordable!) in the past years. It is amazing to see how RBC grows bigger

and stronger over the years!”

“I really appreciate the quick turnaround time from your team in generating a quote for us! (We were in the midst of grant preparation for the next phase of our project.)”

“Thanks for getting the results to my client in such a timely way. They were also impressed with the speed”

“To the Reaction Biology team,Many thanks for the high quality dataset and the rapid turnaround time. The data was

received well.”

“Thank you very much for the data. It caused so much excitement I forgot to thank you! The results are very consistent with data you generated previously.”

“I am currently working with the EZH2 complex that I got from your company. It is beautifully working in my in vitro assay and is a very pure prep.”

t. · lck lck2/ick nek2 nek3 nek4 nek5 nek6 nek7 nek9 nek11 nim1 nlk osr1/oxsr1 p38a/mapk14...

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