Systems and Solutions forEM Sample PreparationThe New Product Portfolio from Leica Microsystems

EM Sample Preparation –Biological Samples

Ultramicrotomy

Leica EM UC7

Leica EM FC7

Leica EM KMR3

Contrasting Leica EM AC20

Processing

Leica EM AFS2/EM FSP

Leica EM TP

Leica EM AMW

TrimmingLeica EM TRIM2

Leica EM RAPID

Cryo Fixation

Leica EM PACT2/EM RTS

Leica EM HPM100

Leica EM GP

Leica EM CPC

Leica EM SPF

Freeze Etching/Freeze Fracture Leica EM BAF060

Cryo Transfer Leica EM VCT100

CoatingLeica EM ACE200

Leica EM ACE600

Drying Leica EM CPD300

The New Product Portfolio from Leica Microsystems

❙❘ Contrasting

Leica EM AC20Automatic contrasting of ultrathin sectionsfor electron microscopy.

• Enhanced safety

• Low reagent consumption

• High contrast

• Reproducible results

❙❘ Processing

Leica EM AFS2Freeze substitution and low temperatureembedding for light and electron microscopy.

• –140°C to +70°C working range

• Transfer function

• Fast LED UV polymerization

• Intuitive programming with USB port

• Stereomicroscope

• Fume exhaust system

• Mouse-controlled color screen

• Deep freeze to –140°C

• Mobile with clamping wheels

Leica EM FSPAn automatic reagent handling/dispensingsystem for freeze substitution and PLT.

• One step preparation

• Safe and convenient handling

• Flexible built-in UV light forpolymerization

• Up to 20 samples per run

Leica EM TPAutomated routine tissue processor.

• Safe

• Time saving

• Reproducible results

• Pre-heating and pre-cooling ofthe reagents

• Versatile: EM, EM high throughput and LM

❙❘ Ultramicrotomy

Leica EM UC7Ultramicrotome for ultrathin sectioning of biological and industrial samples

• Up to 100 different user/specimen/knife profi les can be set

• Electronic data transfer for reporting user, specimen, knife and storage parameters

• Knife usage monitoring

• Optimized ionizer

• Fully motorized knife stageand AutoTrim function

• Brightness-controlledmulti-LED illuminationand LED spot illumination

Leica EM FC7Low temperature sectioning system for ultrathin cryosectioning ofbiological and industral samples.

• Ergonomic design for fatigue-free operation

• Internal chamber illumination

• Temperature range from –185°C to –15°C

• Individual temperature for specimen, knife and gas

• Setting of temperature difference up to 130°C(between knife and specimen)

• GN2 gap between chamber andarm rest ensures a warm surfacefor the user contact

• Heated chamber walls preventicing over a prolongedworking time

• Easy section collectionusing micromanipulatorand EM CRION ionizer

Leica EM KMR3Balanced-break glass knife maker for producing 45° glass knivesfrom 6.4 mm, 8 mm and 10 mm glass.

• Highly reproducible, outstandingknife quality

• Automatic reset of thebreaking andscoring mechanism

• Ergonomic design forcomfortable use

• Easy to learn Versatile: EM, EM high throughput and LM

crosystemsLeica EM HPM100

High pressure freezing system for freezing of specimens up to 200 μm in

thickness and 6 mm in diameter without ice crystal damage.

• High cooling rates

• No cryo protectant required

• Short handling time before

freezing

• Easy one button operation

• Integrated stereomicroscope,

LED, workstation

• Dewar with drain

• USB interface for data storage

and transfer

Leica EM SPF

Self Pressure Freezer is a system suited for:

• Cryo-immobilization of biological samples

• Follow-on procedures for cryo- and room

temperature TEM

• Cryoprotectant

• Suited for CEMOVIS and freeze

substitution follow-on techniques

• Defi ned area of well frozen

samples

Leica EM CPC

Universal cryofi xation system for immersion

and metal mirror cryofi xation.

• Versatile

• Easy change of modules

• Schematic display

• Also for use as temperature controlled

cryopreparation chamber

• Safety alarm

Leica EM AMW

Automatic microwave tissue

processor for electron microscopy.

• Fast processing

• Minimized user interaction

• From fresh tissue to TEM in

the same day

❙❘ Trimming

Leica EM TRIM2

Specimen trimming device for TEM, SEM, LM.

• 1 μm step advance

• Perpendicular viewing of the sample

• LED illumination

Leica EM RAPID

Advanced specimen trimming device

for TEM, SEM, LM.

• 0.5,1,10,100 μm step advance

• Adjustable cutting speed 300–20000 rpm

• Advance indication on LCD display

• Unique system for preparation

of tablets for pharma industry

as well as for advanced

specimen trimming

❙❘ Cryo Fixation

Leica EM PACT2 with EM RTS

High pressure freezer with rapid transfer system.

• Freezing of specimens, up to 200 μm in thickness and 1.5 mm

in diameter without ice crystal damage

• High cooling rates by strong jet of LN2

• Rapid turnaround time

• Compact and mobile

• Automatic bake-out cycle

• Internal memory with

download on USB

• Dewar with drain

• Low LN2 consumption

• Low noise

• Suitable for correlative LM/EM

••

••

L

S

••

LM.

p to 200 μm in thickness and 1.5 mm

rystal damage

ng jet of LN2

M/EM

Leica EM GP

Automatic plunge freezer for the bare grid

technique.

• Programming allows reproducible processes

in a controlled sample environment

• Filling the secondary cryogen is fast,

easy and safe with the unique

liquefying head

• Single sided parallel blotting gives

an even fi lm thickness

❙❘ Coating

Leica EM ACE200

High quality desk-top coater designed to produce homogeneous coatings

of conductive metal or carbon as required for electron microscopy. The

fully automated instrument can be confi gured either as a sputter coater

or a carbon thread evaporation coater. Or, if preferred, the Leica EM

ACE200 can combine both methods with interchangeable heads on the

one instrument. Additional options include:

• Quartz crystal measurement –

for reproducible layers

• Planetary rotation – for even distribution

of coating material on fi ssured samples

• Glow discharge – to make TEM grids

hydrophilic

• Exchangeable shielding – for easy cleaning

Leica EM ACE600

The Leica EM ACE600 is a versatile high vacuum fi lm deposition system,

designed to produce very thin, fi ne-grained and conductive metal and

carbon coatings for the highest resolution analysis, as required for FE-SEM

and TEM applications. This fully automated table-top coater includes an

integrated oil-free vacuum system, quartz crystal fi lm thickness measure-

ment and a motorized stage (rotation, optional tilt and height). The Leica

EM ACE600 can be confi gured for the following methods:

• Sputtering

• Carbon thread evaporation

• Carbon rod evaporation (with an option

for thermal resistance evaporation)

• e-beam evaporation

• Glow discharge

• Leica EM VCT100 adaptation for cryo-

coating, freeze-fracture, double-replica,

freeze-drying and controlled environmental

transfer with the VCT Shuttle

Leica EM ACE600 with Leica EM VCT100

The Leica EM ACE600 outfi tted with a Leica EM VCT100 (vacuum cryo

transfer system) is the ideal solution for contamination-free cryo-SEM

sample preparation with complete environmental control. Cryo stage and

room temperature stages are interchangeable once the coater is cryo-

confi gured. With the Leica EM VCT100 confi guration, the Leica EM ACE600

connects to other VCT-outfi tted instruments such as glove boxes, SEMs

and the Leica EM UC7 with the Leica EM FC7T cryo chamber.

• Transfer under controlled environment

(vacuum and temperature)

• Cryo pump for higher vacuum

• Temperature-controlled stage for

freeze fracture, sublimation and freeze

drying

• Fracturing knife

with motor-driven height adjustment

• Scalpel for basic fracturing

• Double replica release

❙❘ Cryo Transfer

Leica EM VCT100

Versatile cryo vacuum transfer system enabeling transfer of specimen

between different preparation units and an analysis instrument via a

shuttle.

• Contamination-free transfer

• Suitable for either room temperature

or cryo transfer

• Preparation and analysis

can be performed

independently

• Light-weight, space saving shuttle design

offers minimal interference with the analysis instrument –

no vibrations

• Various sample holders for SEM, FIB-SEM, freeze-fracture and more

❙❘ Freeze Etching/Freeze Fracture

Leica EM BAF060

High-end preparation unit, featuring an advanced microtome, fl exible

shadowing options with electron beam sources and a load-lock transfer

system for:

• Freeze-fracture/etching

• Double replica (mirror fracturing)

• Freeze drying

• High resolution carbon/metal mix

coatings for TEM/SEM analysis

• Double layer coating of specimens

for cryo-SEM analysis

• Cryo coating for cryo-SEM using

the Leica EM VCT100

❙❘ Drying

Leica EM CPD300

The new Leica EM CPD300 dries specimens such as pollen, tissue, plants

and insects as well as industrial samples, such as Micro Electro

Mechanical Systems (MEMS), in a fully automated and controlled process.

This automated, controlled technique leads to perfect, reproducible

results and ensures the same sample quality from every run.

• Fully reproducible processes through

full automation

• Reduced process times

through Leica fi ller/sample

holder concept

• Minimized CO2 consumption

and minimal user interaction

time

• Integrated waste separator avoids direct contact with chemical waist

❙❘ Solid State Technology

Leica EM TXP

Target preparation device for milling, sawing,

grinding and polishing.

• Accurate location and preparation

of microtargets

• In-situ stereomicroscope

observation

• Automatic process control to

produce a mirror-like surface quality

Leica EM TIC 3X

The Triple Ion Beam Cutter, Leica EM TIC 3X allows production of cross

sections of hard/soft, porous, heat sensitive, brittle and heterogeneous

material for Scanning Electron Microscopy (SEM), Microstructure

Analysis (EDS, WDS, Auger, EBSD).

• Various sample holders for

almost every sample size

• Broad and deep cutting at high

speed for top-rate performance

• Individual confi gurations

possible by using

interchangeable stages

– Standard stage, Multiple

sample stage or Cooling stage

• Cooling Stage – High quality, low temperature processing of heat

sensitive samples such as rubber and water-soluble polymer fi bers can

be prepared by cooling down the sample holder and mask to -50°C

Leica EM RES102

Unique ion beam milling device that has two modifi ed saddlefi eld

ion sources with variable ion energy for optimum milling results. Like

no other instrument on the market, it combines the preparation of TEM,

SEM and LM samples in one single benchtop unit. In addition to

high-energy milling, the Leica EM RES102 can

also be used for the very gentle sample

processing using low ion energy.

• One single benchtop unit for TEM,

SEM and LM sample preparation

• External control of the miling

process via LAN

• Preparation of samples up

to 25 mm diameter

• Fully computer-controlled

milling parameters

Ultramicrotomy

Leica EM UC7

Leica EM FC7

Leica EM TRIM2

Leica EM KMR3

Leica EM RAPID

Cryo Fixation

Leica EM PACT2/EM RTS

Leica EM HPM100

Leica EM GP

Leica EM CPC

Freeze Etching/Freeze Fracture Leica EM BAF060

Cryo Transfer Leica EM VCT100

CoatingLeica EM ACE200

Leica EM ACE600

Solid State Technology

Leica EM TXP

Leica EM TIC 3X

Leica EM RES102

Drying Leica EM CPD300

EM Sample Preparation –Industrial Samples

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