sympathetic denervation in the mice ~ my life in san diego
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Sympathetic denervation in the mice ~ My life in San Diego. 生科 04 楊慧儒 891644 2002.12.06. 劉新光 教授 John H. K. Liu, Ph.D.. the regulation of intraocular pressure An elevated intraocular pressure ─ primary open-angle glaucoma - PowerPoint PPT PresentationTRANSCRIPT
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Sympathetic denervation in the mice
~ My life in San Diego
生科 04 楊慧儒 891644
2002.12.06
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劉新光 教授 John H. K. Liu, Ph.D.
the regulation of intraocular pressure An elevated intraocular pressure ─
primary open-angle glaucoma the circadian (24-hour) rhythms of intraocular pr
essure and related ocular functions. Human study─ eye drop for glaucoma
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Summer research
Superior cervical ganglionectomy Way to measure the pupil size of the mice Test of eye drop on postsurgical mice
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Superior Cervical Ganglion (SCG)
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Superior Cervical Ganglion (SCG)
Sympathetic nerve─change in pupillary reflex Removal or sectioning of this part can reduce th
e circadian IOP elevation The outcome of removal of SCG or cutting the c
ervical sympathetic trunk will mimic human Horner’s syndrome─drug test
PAST~ Palpebral ptosis and become irreversible from 20th hr Palpebral retraction
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Pharmacology of the sympathetic nervous system
hydroxyamphetamine
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Drug to Test Horner’s Syndrome
Hydroxyamphetaine(HA)
& Tyramine(TA)
Dilation→
no dilation
Postganglionic
Cocaine(C) Dilation→
No dilation
Pre-,postganglionic
Epinephrine(E) No dilation→
Dilation
Postganglionic
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Ganglionectomy
Mouse(C57BL/6)
Male, 30g, 4 mice
Anesthesia
(Ketamine+Xylazine)
Fix it on the bench and preparation of instruments
Surgery
Toe & tail
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Ganglionectomy
Shave Incision Find SCG Cut SCG (right side) Suture back the fats &
the skin
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Ganglionectomy
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Measurement of pupil size
Anesthetized Ketamine pentobarbital
Conscious Tube Cone
Ruler
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Eye Drop Test
Solution (saline) 1 micro-liter, twice, on each eye Record every 5-10mins Experimental time: 70mins
M: mouse number M0: normal mouse (day): the day after surgery
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Result- Hydroxyamphetamine & Tyramine
Conscious Ketamine Pentobarbital
HA (1%) M1(9),M2(8),
M0
M1(7),M2(6) M1(10)
HA (0.1%) M2(7)
TA (5%) M1(15),M2(14),M3(7),M0
TA (1%) M1(16),M2(15),M3(8)
TA (0.1%) M1(17),M2(16),M3(9)
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Result- Cocaine
Conscious Pentobarbital
Cocaine M1(18),
M2(17),
M4(1,3),M0
M2(9)
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Result- Epinephrine
Conscious
Epinephrine 0.1% M1(10)
M2(13)
M3(6,10,15)
M4(8)
M0
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Discussion
Compare to rabbits: Cocaine─ 5~30mins Hydroxyamphetamine─ 30~45mins
Cocaine, hydroxyamphetamine, and tyramine work on rabbits but not mice.
Only using epinephrine at certain day after surgery(10th-15th days) can have significant result to confirm the success of surgery.
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Furthermore
Increase the number of experiment Find a critical way to measure the pupil size of
mice Electrode stimulation The mechanism of drug effect
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Life in San Diego
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Life in San Diego
Balboa park
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Life in San Diego
Mission bay & Balboa park
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University of California,San Diego
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Campus
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