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EUROPEAN COMMISSION HEALTH AND CONSUMERS DIRECTORATE-GENERAL Director General
SANCO/10254/2013
Programmes for the eradication, control and monitoring of certain animal diseases and zoonoses
Survey programme for Avian Influenza
Czech Republic
Approved* for 2013 by Commission Decision 2012/761/EU
* in accordance with Council Decision 2009/470/EC
Commission européenne, B-1049 Bruxelles / Europese Commissie, B-1049 Brussel - Belgium. Telephone: (32-2) 299 11 11.
Page 2 sur 32
Standard requirements for the submission of surveillance programmes for avian influenzaversion : 2.2
1. Identification of the programme
Member state : CESKA REPUBLIKA
Disease : avian influenza in poultry and wild birds
Request of Community co-financing from beginning of : 2013 to end of 2013
1.1 Contact
Name : Petr Šatrán
Phone : +420 227 010 150
Fax. : +420 227 010 195
Email : [email protected]
2. Description and implementation of the surveillance programme in poultry
2.1.1 Designation of the central authority in charge of supervising and coordinating the departments responsible for implementing the programme
(max. 32000 chars) :
The central authority responsible for the programme is the Central Veterinary Administration, Department of Animal Health and Welfare. The plan with the number of sampled holdings in each category of poultry in every region is made by the Central Veterinary Administration. Implementation of the programme is performed by Regional Veterinary Administrations (RVA). The particular tested holdings are chosen by RVA. The samples are taken by veterinary officer or by private veterinarian who provides veterinary care regularly in the tested holding. The private veterinarian have to make a contract with RVA. Only three laboratories are involved into the programme. These laboratories are State Veterinary Institute in Prague (National Reference Laboratory for Avian Influenza), State Veterinary
Page 3 sur 32
Standard requirements for the submission of surveillance programmes for avian influenzaversion : 2.2
Institute in Jihlava and State Veterinary Institute in Olomouc. They are under supervision of the Central Veterinary Administration. In the programme, the external agencies or laboratories are not involved.
2.1.2 System in place for the registration of holdings
(max. 32000 chars) :
Holdings in the Czech Republic are registered in the Database of Farms in accordance to the provisions of the law No. 154/2000, Breeding Act and corresponding Decree No. 136/2004 laying down details for identification of animals and their registration and registration of holdings and person established by Breeding Act. Each holding where a flock is kept of more than 100 head of poultry produced hatching eggs and laying hens (Gallus gallus) produced table eggs witch are marketed too and each holding where a flock is kept of more than 500 head of poultry shall be assigned, by the entrusted person, by a registration number of the holding, and all farmers’ records shall be kept in accordance with Breeding Act and Decree No 136/2004, as amended.
2.1.3 Design (risk based or surveillance based on representative sampling)
(max. 32000 chars) :
The Czech Republic is out of the main migratory flight paths of birds from central and eastern Asia, the Caspian Sea and the Black Sea areas. Due to this reason and according to the epidemiological and ornithological data we have not identified any region at higher risk for the introduction of AI. There are identified only areas with high density of poultry which is kept in an open air holdings close to water areas in the Czech Republic. For these reasons, the Czech Republic does not carry out risk based surveillance for surveillance for avian influenza. Surveillance based on a representative sampling scheme is implemented in the Czech Republic. Generally, the programme is applied on the whole territory of the Czech Republic, so that samples are considered as representative for the whole of the Czech Republic. The programme is implemented in all 14 regions in the Czech Republic. All regions are divided into the smaller areas which are named as districts. Programme is implemented on the regional level and Regional Veterinary Administrations (RVA) are in charge for performing of the programme.
2.1.3.1 Short description of predominant poultry population and types of poultry production
Page 4 sur 32
Standard requirements for the submission of surveillance programmes for avian influenzaversion : 2.2
(max. 32000 chars) :
Holdings where the fattening broilers flocks of Gallus gallus and laying hens (Gallus gallus) produced table eggs are kept represent the main type of poultry production in the Czech Republic. There is seasonal production of the fattening turkeys before Christmas and Easter and small numbers of holdings with fattening ducks and geese. Table eggs are produced in relatively large amount in backyard flocks, especially in the country.
2.1.3.2 Criteria and risk factors for risk based surveillance(1)
(max. 32000 chars) :
The Czech Republic does not carry out risk based surveillance method for surveillance for avian influenza. Surveillance based on a representative sampling scheme is implemented in the Czech Republic.
(1) Including maps showing target sampling sites identified as being particularly at risk for the introduction of avian influenza virus, taking into account criteria set out in point 4 of Annex I to Commission Decision 2010/367/EC.
2.2 Target populations (2)
(max. 32000 chars) :
The surveillance programme for avian influenza in poultry will be included these categories: (a) laying hens; (b) free range laying hens; (c) turkey breeders; (d) duck breeders; (e) geese breeders; (f) fattening turkeys; (g) fattening ducks; (h) fattening geese; (i) farmed game birds (gallinaceous) ; (j) farmed game birds (waterfowl);
Page 5 sur 32
Standard requirements for the submission of surveillance programmes for avian influenzaversion : 2.2
(2) including MS specific exceptional circumstances as described in Annex I point 3 of Commission Decision 2010/367/EU)
Page
6 su
r 32
versio
n : 2
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Stan
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Page
7 su
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8 su
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10 su
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11 su
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12 su
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Sero
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Ann
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mm
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13 su
r 32
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n : 2
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Stan
dard
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Page
14 su
r 32
versio
n : 2
.2
Stan
dard
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for t
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20EL
ISA
test
xC
Z 06
2 So
uth
Mor
avia
11
2020
ELIS
A te
stx
CZ
071
Olo
mou
c1
120
20EL
ISA
test
x
Tota
l7
780
140
Add
a n
ew ro
w
(a)
Hol
ding
s or h
erds
or f
lock
s or e
stab
lishm
ents
as a
ppro
pria
te.
(b)
Refe
rs to
the
loca
tion
of th
e ho
ldin
g of
orig
in. I
n ca
se N
UTS
(2) c
ode
can
not b
e us
ed, r
egio
n as
def
ined
in th
e pr
ogra
mm
e by
the
Mem
ber S
tate
is re
ques
ted
Cate
gory
: fa
tteni
ng g
eese
dele
te t
his
cate
gory
NU
TS (2
) (b)
Tota
l num
ber o
f duc
k an
d
gees
e ho
ldin
gs
Tota
l num
ber o
f duc
k an
d ge
ese
hold
ings
to b
e sa
mpl
ed
Num
ber o
f sam
ples
per
ho
ldin
gTo
tal n
umbe
r of t
ests
Met
hod
of la
bora
tory
ana
lysis
CZ
031
Sout
h Bo
hem
ia2
220
40EL
ISA
test
x
Page
15 su
r 32
versio
n : 2
.2
Stan
dard
requ
irem
ents
for t
he su
bmis
sion
of
surv
eilla
nce
prog
ram
mes
for a
vian
influ
enza
CZ
061
Vysočin
a1
120
20EL
ISA
test
xC
Z 08
1 M
orav
ia-S
ales
ian
11
2020
ELIS
A te
stx
Tota
l4
460
80
Add
a n
ew ro
w
(a)
Hol
ding
s or h
erds
or f
lock
s or e
stab
lishm
ents
as a
ppro
pria
te.
(b)
Refe
rs to
the
loca
tion
of th
e ho
ldin
g of
orig
in. I
n ca
se N
UTS
(2) c
ode
can
not b
e us
ed, r
egio
n as
def
ined
in th
e pr
ogra
mm
e by
the
Mem
ber S
tate
is re
ques
ted
Cate
gory
: fa
rmed
gam
e (w
ater
fow
l e.g
. mal
lard
s)de
lete
thi
s ca
tego
ry
NU
TS (2
) (b)
Tota
l num
ber o
f duc
k an
d
gees
e ho
ldin
gs
Tota
l num
ber o
f duc
k an
d ge
ese
hold
ings
to b
e sa
mpl
ed
Num
ber o
f sam
ples
per
ho
ldin
gTo
tal n
umbe
r of t
ests
Met
hod
of la
bora
tory
ana
lysis
CZ
031
Sout
h Bo
hem
ia9
920
180
ELIS
A te
stx
CZ
032
Plzeň
22
2040
ELIS
A te
stx
CZ
062
Sout
h M
orav
ia1
120
20EL
ISA
test
xC
Z 07
1 O
lom
ouc
11
2020
ELIS
A te
stx
CZ
081
Mor
avia
-Sile
sian
11
2020
ELIS
A te
stx
Tota
l14
1410
028
0
Page
16 su
r 32
versio
n : 2
.2
Stan
dard
requ
irem
ents
for t
he su
bmis
sion
of
surv
eilla
nce
prog
ram
mes
for a
vian
influ
enza
Add
a n
ew ro
w
(a)
Hol
ding
s or h
erds
or f
lock
s or e
stab
lishm
ents
as a
ppro
pria
te.
(b)
Refe
rs to
the
loca
tion
of th
e ho
ldin
g of
orig
in. I
n ca
se N
UTS
(2) c
ode
can
not b
e us
ed, r
egio
n as
def
ined
in th
e pr
ogra
mm
e by
the
Mem
ber S
tate
is re
ques
ted
Add
a c
ateg
ory
Tota
l Du
cks
and
gees
e64
6446
01
280
Gra
nd T
otal
Po
ultry
+ D
ucks
/Gue
ese
282
189
840
2 53
0
Page
17 su
r 32
versio
n : 2
.2
Stan
dard
requ
irem
ents
for t
he su
bmis
sion
of
surv
eilla
nce
prog
ram
mes
for a
vian
influ
enza
2.3
Sa
mpl
ing
proc
edur
es, s
ampl
ing
perio
ds a
nd fr
eque
ncy
of te
stin
g
(max
. 320
00 c
hars
) :
Bloo
d sa
mpl
es fo
r ser
olog
ical
exa
min
atio
n w
ill b
e co
llect
ed fr
om la
ying
hen
s, fr
ee ra
nge
layi
ng h
ens,
turk
ey b
reed
ers,
duc
k br
eede
rs, g
eese
bre
eder
s,
fatt
enin
g tu
rkey
s, fa
tten
ing
duck
s, fa
tten
ing
gees
e, fa
rmed
gam
e bi
rds
wat
erfo
wl a
nd g
allin
aceo
us. T
he b
lood
sam
ples
for s
erol
ogic
al te
stin
g w
ill b
e ta
ken
in th
e ho
ldin
gs. W
hen
the
poul
try
will
be
disp
atch
ed fo
r sla
ught
erin
g ab
road
, the
sam
ples
will
be
take
n in
the
hold
ing
befo
re d
ispa
tch.
Sa
mpl
es fr
om 1
0 bi
rds
(exc
ept d
ucks
, gee
se a
nd fa
rmed
gam
e bi
rds
wat
erfo
wl)
will
be
take
n in
eac
h te
sted
hol
ding
and
from
the
diffe
rent
she
ds, i
f mor
e th
an o
ne s
hed
will
pre
sent
on
a ho
ldin
g.
In d
ucks
, gee
se a
nd fa
rmed
gam
e bi
rds
wat
erfo
wl h
oldi
ngs
will
be
sam
pled
20
bird
s fr
om th
e di
ffere
nt s
heds
, if m
ore
than
one
she
d w
ill b
e pr
esen
t on
a ho
ldin
g.
Bird
s in
the
poul
try
hold
ings
will
be
sam
pled
con
tinuo
sly
durin
g al
l yea
r 201
3. T
he ti
me
perio
d fo
r sam
plin
g in
the
poul
try
hold
ing
coin
cide
with
sea
sona
l pr
oduc
tion
for e
ach
poul
try
prod
uctio
n ca
tego
ry. S
easo
nal p
rodu
ctio
n is
the
mos
t dis
tinct
ive
by fa
tten
ing
turk
eys,
duc
ks a
nd g
eese
, usu
ally
bef
ore
Chris
tmas
and
Eas
ter.
How
ever
, whe
re a
ppro
pria
te, s
ampl
ing
will
be
adap
ted
to o
ther
iden
tifie
d pe
riods
. The
sam
plin
g of
all
poul
try
cate
gorie
s w
ill b
e ca
rrie
d ou
t onc
e du
ring
2013
.
2.4.
La
bora
tory
test
ing
: des
crip
tion
of th
e la
bora
tory
test
s use
d an
d fo
llow
up
inve
stig
atio
ns
Page
18 su
r 32
versio
n : 2
.2
Stan
dard
requ
irem
ents
for t
he su
bmis
sion
of
surv
eilla
nce
prog
ram
mes
for a
vian
influ
enza
Des
crip
tion
of th
e us
ed se
rolo
gica
l tes
ts :
(max
320
00 c
hars
)
Labo
rato
ry te
sts
will
be
carr
ied
out i
n ac
cord
ance
with
the
avia
n in
fluen
za d
iagn
ostic
man
ual (
Com
mis
sion
Dec
isio
n 20
06/4
37/E
C) la
ying
dow
n th
e pr
oced
ures
for t
he c
onfir
mat
ion
and
diffe
rent
ial d
iagn
osis
of a
vian
influ
enza
. EL
ISA
test
for i
nitia
l scr
eeni
ng a
nd H
IT fo
r con
firm
atio
n w
ill b
e us
ed in
sam
pled
pou
ltry
hold
ings
.
Test
s La
ying
hen
s, fr
ee ra
nge
layi
ng h
ens
and
turk
eys
(bot
h br
eede
rs a
nd fa
tten
ing)
EL
ISA
test
(gro
up a
ntig
en),
HI t
est (
typi
ng w
ith H
5 an
d H
7 su
btyp
es o
f ant
igen
). Fo
r ELI
SA te
st w
ill b
e us
ed A
VIA
N IN
FLU
ENZA
AN
TIBO
DY
TEST
Kit
prod
uced
by
IDEX
X la
bora
torie
s (U
S).
In th
e ca
se o
f pos
itive
reac
tion,
typi
ng b
y H
I tes
t will
be
perf
orm
ed (t
ypin
g w
ith H
sub
type
s of
ant
igen
). Fo
r HIT
will
be
used
ant
igen
s pr
ovid
ed b
y CR
L in
W
eybr
idge
. D
ucks
and
gee
se (b
oth
bree
ders
and
fatt
enin
g), f
arm
ed g
ames
bird
s w
ater
fow
l EL
ISA
test
(gro
up a
ntig
en),
HI t
est (
typi
ng w
ith H
5 an
d H
7 su
btyp
es o
f ant
igen
). Fo
r ELI
SA te
st h
as b
een
used
AVI
AN
INFL
UEN
ZA V
IRU
S A
NTI
BOD
Y TE
ST K
it, E
LISA
(Mul
ti-Sc
reen
) pro
duce
d by
IDEX
X la
bora
torie
s (U
S).
In th
e ca
se o
f pos
itive
reac
tion,
typi
ng b
y H
I tes
t wou
ld b
e pe
rfor
med
(typ
ing
with
H s
ubty
pes
of a
ntig
en).
All
posi
tive
sero
logi
cal f
indi
ngs
shal
l be
conf
irmed
by
the
NRL
at S
VI P
ragu
e fo
r avi
an in
fluen
za b
y a
haem
aggl
utin
atio
n-in
hibi
tion
test
, usi
ng d
esig
nate
d st
rain
s su
pplie
d by
the
Com
mun
ity R
efer
ence
Lab
orat
ory
for A
vian
Influ
enza
: H
5
(a) I
nitia
l tes
t usi
ng te
al/E
ngla
nd/7
894/
06 (H
5N3)
(b
) Tes
t all
posi
tives
with
chi
cken
/Sco
tland
/59
(H5N
1) to
elim
inat
e N
3 cr
oss
reac
tive
antib
ody.
H
7
(a) I
nitia
l tes
t usi
ng T
urke
y/En
glan
d/64
7/77
(H7N
7)
(b) T
est a
ll po
sitiv
es w
ith A
fric
an S
tarli
ng/9
83/7
9 (H
7N1)
to e
limin
ate
N7
cros
s re
activ
e an
tibod
y.
Page
19 su
r 32
versio
n : 2
.2
Stan
dard
requ
irem
ents
for t
he su
bmis
sion
of
surv
eilla
nce
prog
ram
mes
for a
vian
influ
enza
Test
ing
of s
ampl
es w
ill b
e ca
rrie
d ou
t in
the
Nat
iona
l Ref
eren
ce L
abor
ator
y (N
RL) i
n th
e S
tate
Vet
erin
ary
Inst
itute
(SVI
) in
Prag
ue a
nd in
SVI
in O
lom
ouc
and
SVI i
n Ji
hlav
a. T
he la
bora
torie
s in
SVI
Olo
mou
c an
d SV
I Jih
lava
are
und
er c
ontr
ol o
f the
NRL
. The
NRL
coo
pera
tes
with
Com
mun
ity R
efer
ence
Lab
orat
ory
for A
vian
Influ
enza
(CRL
) in
Wey
brid
ge.
All
resu
lts w
ill b
e se
nt to
the
Com
mun
ity R
efer
ence
Lab
orat
ory
for A
vian
Influ
enza
(CRL
) fo
r col
latio
n.
3.
Des
crip
tion
and
impl
emen
tatio
n of
the
surv
eilla
nce
prog
ram
me
in w
ild b
irds
3.1.
1 D
esig
natio
n of
the
cent
ral a
utho
rity
in c
harg
e of
supe
rvis
ing
and
coor
dina
ting
the
depa
rtm
ents
resp
onsi
ble
for i
mpl
emen
ting
the
prog
ram
me
and
rele
vant
col
labo
ratin
g pa
rtne
rs (e
.g. e
pide
mio
logi
sts,
orni
thol
ogis
ts, n
atur
e bi
rd o
bser
vatio
n an
d hu
nter
or
gani
satio
ns).
(max
. 320
00 c
hars
) :
The
cent
ral a
utho
rity
resp
onsi
ble
for t
he p
rogr
amm
e is
the
Cent
ral V
eter
inar
y A
dmin
istr
atio
n, D
epar
tmen
t of A
nim
al H
ealth
and
Wel
fare
. Im
plem
enta
tion
of th
e pr
ogra
mm
e is
per
form
ed b
y Re
gion
al V
eter
inar
y A
dmin
istr
atio
ns (R
VA).
Pass
ive
surv
eilla
nce
on s
ick
and
dead
wild
bird
s is
per
form
ed in
the
Czec
h Re
publ
ic. R
VA c
oope
rate
with
orn
ithol
ogis
ts, n
atur
e bi
rd o
bser
vatio
n an
d hu
nter
org
anis
atio
ns in
acc
orda
nce
with
Art
icle
1, C
omis
sion
Dec
isio
n N
o 36
7/20
10. T
rain
ig c
ours
e fo
r the
se o
rgan
isat
ions
are
org
aniz
ed b
y RV
A in
eac
h re
gion
eve
ry y
ear.
3.1.
2 D
escr
iptio
n an
d de
limita
tion
of th
e ge
ogra
phic
al a
nd a
dmin
istr
ativ
e ar
eas i
n w
hich
the
prog
ram
me
is to
be
appl
ied
Page
20 su
r 32
versio
n : 2
.2
Stan
dard
requ
irem
ents
for t
he su
bmis
sion
of
surv
eilla
nce
prog
ram
mes
for a
vian
influ
enza
max
. 320
00 c
hars
) :
The
prog
ram
me
will
be
appl
ied
in a
ll te
rrito
ry o
f the
Cze
ch R
epub
lic a
ccor
ding
to ru
les
sett
ing
by th
e Ce
ntra
l Vet
erin
ary
Adm
inis
trat
ion.
Re
gion
s in
the
Czec
h Re
publ
ic w
here
the
prog
ram
me
is to
be
appl
ied:
CZ
011
CA
PITA
L CI
TY P
RAG
UE
CZ
021
CEN
TRA
L BO
HEM
IAN
REG
ION
CZ
031
SO
UTH
ERN
BO
HEM
IAN
REG
ION
CZ
032
REG
ION
OF
PLZE
N
CZ 0
41 R
EGIO
N O
F KA
RLO
VY V
ARY
CZ
042
REG
ION
OF
UST
I N. L
ABE
M
CZ 0
51 R
EGIO
N O
F LI
BERE
C
CZ 0
52 R
EGIO
N O
F H
RAD
EC K
RALO
VE
CZ 0
53 R
EGIO
N O
F PA
RDU
BICE
CZ
061
REG
ION
OF
VYSO
CIN
A
CZ 0
62 S
OU
THER
N M
ORA
VIA
N R
EGIO
N
CZ 0
71 R
EGIO
N O
F O
LOM
OU
C CZ
072
REG
ION
OF
ZLIN
CZ
081
MO
RAVI
A-S
ILES
IAN
REG
ION
3.1.
3 Es
timat
ion
of th
e lo
cal a
nd/o
r mig
rato
ry w
ildlif
e po
pula
tion
(max
. 320
00 c
hars
) :
The
Czec
h Re
publ
ic is
out
of t
he m
ain
mig
rato
ry fl
ight
pat
hs o
f bird
s fr
om c
entr
al a
nd e
aste
rn A
sia,
the
Casp
ian
Sea
and
the
Blac
k Se
a ar
eas.
Due
to re
ason
ac
cord
ing
the
epid
emio
logi
cal a
nd o
rnith
olog
ical
dat
a w
e ha
ve n
ot id
entif
ied
any
regi
on a
t hig
her r
isk
for t
he in
trod
uctio
n of
AI.
Ther
e ar
e id
entif
ied
only
re
gion
s w
ith th
e la
rge
wat
ers
area
s w
here
the
maj
ority
of w
ater
fow
l nes
t.
Page
21 su
r 32
versio
n : 2
.2
Stan
dard
requ
irem
ents
for t
he su
bmis
sion
of
surv
eilla
nce
prog
ram
mes
for a
vian
influ
enza
3.2
Des
ign,
crit
eria
, ris
k fa
ctor
s and
targ
et p
opul
atio
n(3)
(max
. 320
00 c
hars
) :
Pass
ive
surv
eilla
nce
on s
ick
and
dead
wild
bird
s w
ill b
e ta
rget
ed o
n:
• bird
s be
long
ing
to id
entif
ied
“hig
her r
isk”
spe
cies
(M
alla
rd, M
ute
Swan
, Gre
ylag
Goo
se, C
orm
oran
t, H
eron
, Com
mon
Gul
l)
• are
as w
here
incr
ease
d m
orta
litie
s oc
cur
• are
as c
lose
to th
e po
nds,
lake
s an
d w
ater
way
s w
here
bird
s w
ere
foun
d de
ad; a
nd in
par
ticul
ar w
hen
thes
e ar
eas
are
in p
roxi
mity
to d
omes
tic p
oultr
y
fa
rms
• in
area
s w
here
cas
es o
f HPA
I H5N
1 ha
ve b
een
iden
tifie
d in
wild
bird
s or
pou
ltry
to p
ossi
bly
iden
tify
asym
ptom
atic
car
riers
• i
n ar
eas
epid
emio
logi
cally
link
ed to
thes
e ca
ses
Viro
logi
cal s
urve
ys fo
r avi
an in
fluen
za in
wild
bird
s w
ill b
e ai
med
at i
dent
ifyin
g th
e ris
k fo
r int
rodu
ctio
n of
AI v
iruse
s (L
PAI a
nd H
PAI)
to d
omes
tic p
oultr
y by
: • e
nsur
ing
of a
n ea
rly d
etec
tion
of H
PAI H
5N1
by in
vest
igat
ing
sick
and
dea
d w
ild b
irds
and
incr
ease
d m
orta
litie
s, in
par
ticul
ar in
sel
ecte
d “h
ighe
r ris
k”
spec
ies
• Ans
erifo
rmes
(wild
fow
l) to
be
the
mai
n sa
mpl
ing
targ
ets
to a
sses
s if
they
car
ry L
PAI v
iruse
s of
H5
and
H7
subt
ypes
(whi
ch w
ill in
any
cas
e al
so d
etec
t H
PAI H
5N1
and
othe
r HPA
I, if
pres
ent)
• i
n ca
se o
f HPA
I H5N
1 is
det
ecte
d in
wild
bird
s in
an
area
, ass
essi
ng s
peci
fical
ly th
e ris
k fo
r the
intr
oduc
tion
of H
PAI H
5N1
to p
oultr
y –
by in
vest
igat
ing
dead
and
livi
ng w
ild b
irds
of o
ther
bird
spe
cies
to p
ossi
bly
iden
tify
asym
ptom
atic
car
riers
and
the
risk
that
spe
cies
com
ing
in c
lose
con
tact
with
dom
estic
po
ultr
y ho
ldin
gs m
ight
func
tion
as “b
ridge
spe
cies
”
Page
22 su
r 32
versio
n : 2
.2
Stan
dard
requ
irem
ents
for t
he su
bmis
sion
of
surv
eilla
nce
prog
ram
mes
for a
vian
influ
enza
• int
ensi
ficat
ion
of s
urve
illan
ce o
f HPA
I H5N
1 fir
stly
in th
ose
area
s w
here
its
pres
ence
has
bee
n co
nfirm
ed.
Map
of H
PAI H
5N1
occu
rren
ces
in w
ild b
irds
is a
ttec
hed.
(3)
Are
as a
t ri
sk (
wet
land
s in
par
ticu
lar
whe
re l
inks
wit
h hi
gh d
ensi
ty p
oult
ry p
opul
atio
ns),
pre
viou
s po
siti
ve f
indi
ngs
as r
efer
red
to i
n po
int
2 of
Par
t 1
of A
nnex
II t
o Co
mm
issi
on D
ecis
ion
2010
/367
/EC
shou
ld b
e ta
ken
into
acc
ount
and
if
poss
ible
com
plem
ente
d by
a m
ap.
3.2.
1 W
ILD
BIR
DS
focu
ssed
on
targ
et sp
ecie
s
Inve
stig
atio
ns a
ccor
ding
to th
e su
rvei
llanc
e pr
ogra
mm
e se
t out
in P
art 2
of A
nnex
II to
Dec
isio
n 20
10/3
67/E
C
NU
TS (2
) cod
e/re
gion
(a)
Wild
bird
s to
be
sam
pled
(b)
Tota
l num
ber o
f bird
s to
be
sam
pled
Estim
ated
tota
l num
ber o
f sa
mpl
es to
be
take
n fo
r act
ive
surv
eilla
nce
(c)
Estim
ated
tota
l num
ber o
f sa
mpl
es to
be
take
n fo
r pas
sive
surv
eilla
nce
the
Cze
ch R
epub
licsic
k an
d de
ad w
ild b
irds,
mai
nly
A30
00
300
x
Tota
l0
300
030
0
Add
a n
ew ro
w
(a)
Refe
rs to
the
plac
e of
col
lect
ion
of b
irds/
sam
ples
. In
case
NU
TS 2
(Nom
encl
atur
e of
Ter
ritor
ial U
nits
for S
tatis
tics)
can
not
be
used
, reg
ion
as d
efin
ed in
the
prog
ram
me
by th
e M
embe
r Sta
te is
requ
este
d. P
leas
e fil
l-in
thes
e va
lues
dire
ctly
in th
e fie
ld.
(b
) G
ener
al d
escr
iptio
n of
the
wild
bird
s are
inte
nded
to b
e sa
mpl
ed in
the
fram
ewor
k of
the
activ
e an
d pa
ssiv
e su
rvei
llanc
e.
(c)
Volu
ntar
y, to
be
incl
uded
for i
nfor
mat
ion
purp
oses
, not
elig
ible
for c
ofin
anci
ng.
Page
23 su
r 32
versio
n : 2
.2
Stan
dard
requ
irem
ents
for t
he su
bmis
sion
of
surv
eilla
nce
prog
ram
mes
for a
vian
influ
enza
3.3
Sam
plin
g pr
oced
ures
and
sam
plin
g pe
riods
max
320
00 c
hars
:
Taki
ng s
ampl
es, w
rapp
ing
and
pack
agin
g of
sam
ples
and
thei
r tra
nspo
rt to
the
NRL
will
be
arra
nged
by
an o
ffici
al v
eter
inar
ian
from
the
rele
vant
Reg
iona
l Ve
terin
ary
Adm
inis
trat
ion.
• I
n th
e ca
se o
f mor
talit
y in
crea
se (m
ore
than
5 b
irds)
at l
east
5 c
arca
sses
to b
e ta
ken.
In th
e ca
se o
f hig
her a
mou
nts
of d
ead
bird
s, c
loac
al a
nd tr
ache
al/
orop
hary
ngea
l sw
abs
form
oth
er b
irds
can
be ta
ken
as w
ell.
• S
wab
s m
ust b
e ch
illed
imm
edia
tely
on
ice
or w
ith fr
ozen
gel
pac
ks a
nd s
ubm
itted
to th
e la
bora
tory
as
quic
kly
as p
ossi
ble.
• C
loac
al s
wab
s an
d tr
ache
al/o
roph
aryn
geal
sw
abs
and/
or ti
ssue
s (n
amel
y th
e br
ain,
hea
rt, l
ung,
kid
ney
and
inte
stin
es) f
rom
wild
bird
s w
ill b
e sa
mpl
ed fo
r vi
rus
isol
atio
n an
d m
olec
ular
det
ectio
n (R
T-PC
R).
3.4
Labo
rato
ry te
stin
g : d
escr
iptio
n of
the
labo
rato
ry te
sts u
sed
max
320
00 c
hars
:
Labo
rato
ry te
sts
will
be
carr
ied
out i
n ac
cord
ance
with
the
avia
n in
fluen
za d
iagn
ostic
man
ual.
All
sam
ples
col
lect
ed in
the
surv
ey fo
r avi
an in
fluen
za in
w
ild b
irds
will
be
test
ed a
s s
oon
as p
ossi
ble
by m
olec
ular
tech
niqu
es a
ccor
ding
to th
e di
agno
stic
man
ual.
Initi
al s
cree
ning
usi
ng M
gen
e PC
R w
ill b
e us
ed,
with
rapi
d te
stin
g of
pos
itive
s fo
r H5
(but
with
in 2
wee
ks) a
nd in
cas
e of
a p
ositi
ve fi
ndin
g an
alys
is o
f the
cle
avag
e si
te s
houl
d be
und
erta
ken
as s
oon
as
poss
ible
to d
eter
min
e w
heth
er o
r not
it h
as a
hig
hly
pat
hoge
nic
avia
n in
fluen
za (H
PAI)
or a
low
pat
hoge
nic
avia
n in
fluen
za (L
PAI).
All
sam
ples
pos
itive
for
M g
ene
to b
e te
sted
by
viru
s is
olat
ion
test
on
eggs
. At t
he la
bora
tory
poo
ling
of u
p to
five
sam
ples
take
n fr
om th
e sa
me
spec
ies
colle
cted
at t
he s
ame
site
an
d sa
me
time
is p
erm
itted
whe
n it
can
be e
nsur
ed th
at, i
n ca
se o
f a p
ositi
ve fi
ndin
g, th
e in
divi
dual
sam
ples
can
be
iden
tifie
d an
d re
test
ed.
Resu
lts, a
s w
ell a
s al
l iso
late
s w
ill b
e se
nt to
the
CRL.
Page
24 su
r 32
versio
n : 2
.2
Stan
dard
requ
irem
ents
for t
he su
bmis
sion
of
surv
eilla
nce
prog
ram
mes
for a
vian
influ
enza
4.
Des
crip
tion
of th
e ep
idem
iolo
gica
l situ
atio
n of
the
dise
ase
in p
oultr
y du
ring
the
last
five
yea
rsm
ax 3
2000
cha
rs :
In 2
011
only
one
hol
ding
with
bre
eder
duc
ks a
nd o
ne h
oldi
ng w
ith b
reed
er g
eese
wer
e se
rolo
gy p
ositi
ve fo
r sub
type
H5.
PCR
test
s w
ere
in b
oth
case
s ne
gativ
e.
Ove
r yea
r 201
0 ei
ther
hig
hly
path
ogen
ic a
vian
influ
enza
or l
ow p
atho
geni
c av
ian
influ
enza
sub
type
H5/
H7
wer
e no
t det
ecte
d w
ithin
the
surv
eilla
nce
in
poul
try
in th
e Cz
ech
Repu
blic
. The
re w
as o
nly
one
posi
tive
hold
ing
of fa
rmed
gam
e bi
rds
(mal
lard
s) fo
r LPA
I sub
type
H6N
9 in
pou
ltry.
In
200
9 th
ere
wer
e de
tect
ed 2
out
brea
ks o
f low
pat
hoge
nic
influ
enza
in p
oultr
y ho
ldin
gs.
Firs
t out
brea
k: H
odon
ín
Low
pat
hoge
nic
avia
n in
fluen
za (L
PAI)
of s
ubty
pe H
7N9
was
det
ecte
d in
pou
ltry
hold
ing
keep
ing
goos
e an
d du
ck fl
ocks
in F
ebru
ary
2009
. Th
e ho
ldin
g ow
ned
by c
omm
erci
al fi
sher
ies
Hod
onín
s. r
. o. i
s si
tuat
ed o
n th
e po
nds
clos
e to
the
city
Hod
onín
, in
Sout
hern
Mor
avia
n re
gion
, dis
tric
t H
odon
in.
On
pond
Pís
ečen
ský
wer
e fo
und
infe
cted
gee
se. S
ampl
es w
ere
take
n in
the
fram
ewor
k of
rout
ine
AI s
urve
illan
ce in
a p
oultr
y ho
ldin
g. T
here
wer
e 2
shed
s of
679
hea
ds o
f gee
se w
ere
kille
d us
ing
CO2
in g
as-t
ight
con
tain
ers
on th
e 25
th F
ebru
ary
2009
. Se
cond
out
brea
k: T
řebí
n LP
AI o
f sub
type
H5N
3 w
as d
etec
ted
in h
oldi
ng p
rodu
cing
cap
tive
mal
lard
s fo
r res
tock
ing
in N
ovem
ber 2
009.
Th
e ho
ldin
g ow
ned
by L
esy
a ry
bník
y m
ěsta
Čes
kých
Bud
ějov
ic s
.r.o.
is s
ituat
ed o
n po
nd R
yšáv
ek n
ear m
unic
ipal
ity T
řebí
n. T
here
wer
e ke
pt 2
80 m
alla
rds
for r
esto
ckin
g. S
ampl
es w
ere
take
n in
the
fram
ewor
k of
rout
ine
AI s
urve
illan
ce in
farm
ed g
ame
bird
s ho
ldin
g.
All
280
mal
lard
s ha
ve b
een
culle
d by
priv
ate
vete
rinar
ian
used
“T 6
1” o
n 6t
h N
ovem
ber a
nd s
afet
y di
spos
ed to
the
rend
erin
g pl
ant.
In 2
008
eith
er h
ighl
y pa
thog
enic
avi
an in
fluen
za (H
PAI)
or lo
w p
atho
geni
c av
ian
influ
enza
(LPA
I) w
ere
not d
etec
ted
in th
e Cz
ech
Repu
blic
in p
oultr
y.
In 2
007
ther
e w
as h
isto
rical
ly fi
rst o
utbr
eak
of H
PAI i
n po
ultr
y ho
ldin
g. T
otal
ly 4
hol
ding
s ow
ned
by tw
o di
ffere
nt fa
rmer
s w
ere
affe
cted
in 2
007;
all
hold
ings
wer
e si
tuat
ed in
Par
dubi
ce R
egio
n, d
istr
ict Ú
stí n
ad O
rlicí
. Due
to a
nim
al h
ealth
reas
ons,
103
378
hea
d of
pou
ltry
(4 5
86 tu
rkey
s an
d a
98 7
92 h
ead
Page
25 su
r 32
versio
n : 2
.2
Stan
dard
requ
irem
ents
for t
he su
bmis
sion
of
surv
eilla
nce
prog
ram
mes
for a
vian
influ
enza
of s
peci
es G
allu
s ga
llus)
of t
otal
wei
ght o
f 358
262
kg
wer
e ki
lled
in c
omm
erci
al fl
ocks
and
ano
ther
two
cont
act h
oldi
ngs
with
the
tota
l num
ber o
f pou
ltry
67 8
79 h
ead
(54
125
broi
lers
and
13
754
turk
eys)
wer
e de
popu
late
d pr
even
tivel
y. F
urth
erm
ore,
it w
as d
ecid
ed to
kill
pre
vent
ivel
y po
ultr
y ke
pt in
non
-co
mm
erci
al fl
ocks
in m
unic
ipal
ities
situ
ated
in c
atas
tral
terr
itorie
s of
HPA
I out
brea
ks (2
026
hea
d of
pou
ltry)
. The
firs
t out
brea
k w
as c
onfir
med
in a
turk
ey
flock
on
21 Ju
ne 2
007,
the
seco
nd o
utbr
eak
was
con
firm
ed in
a b
roile
r flo
ck o
n 27
June
200
7, th
e th
ird a
nd fo
urth
out
brea
ks w
ere
conf
irmed
in
repr
oduc
tion
poul
try
flock
s of
spe
cies
Gal
lus
gallu
s, b
oth
outb
reak
s on
the
sam
e da
y, i.
e. o
n 11
July
200
7. M
easu
res
orde
red
in z
ones
wer
e re
peal
ed o
n 14
A
ugus
t 200
7, in
acc
orda
nce
with
the
legi
slat
ion
in fo
rce.
5.
Des
crip
tion
of th
e ep
idem
iolo
gica
l situ
atio
n of
the
dise
ase
in w
ild b
irds d
urin
g th
e la
st fi
ve y
ears
(max
. 320
00 c
hars
) :
In 2
011,
with
in th
e su
rvei
llanc
e of
wild
bird
s th
ere
wer
e te
sted
624
wild
bird
s. In
the
fram
e of
act
ive
surv
eilla
nce,
ther
e w
ere
test
ed 5
64 h
unte
d m
alla
rds
and
othe
r 60
bird
s w
ere
exam
ined
in th
e fr
ame
of p
assi
ve s
urve
illan
ce. T
here
wer
e 22
wild
bird
s po
sitiv
e te
sted
for v
irus
AI f
rom
wha
t 21
posi
tive
mal
lard
s w
ere
test
ed in
act
ive
surv
eilla
nce
and
1 po
sitiv
e m
ute
swan
was
foun
d de
ad. I
n on
e m
alla
rd, t
here
wer
e 2
posi
tive
sam
ples
, bot
h cl
oaca
l and
trac
heal
sw
ab
(it m
eans
22
posi
tive
swab
s in
21
mal
lard
s).
With
in th
e su
rvei
llanc
e of
wild
bird
s, th
ere
wer
e te
sted
in to
tal 6
53 b
irds
in 2
010.
In th
e fr
ame
of a
ctiv
e su
rvei
llanc
e, th
ere
wer
e te
sted
500
hun
ted
wild
bi
rds
(mal
lard
s) w
here
as 7
6 m
alla
rds
of th
em w
ere
viro
logi
cal p
ositi
ve fo
r LPA
I (on
e po
sitiv
e fin
ding
of L
PAI H
5N3)
. Oth
er 1
53 w
ild b
irds
wer
e ex
amin
ed fo
r av
ian
influ
enza
in th
e fr
ame
of p
assi
ve s
urve
illan
ce (f
ound
dea
d). O
nly
one
bird
(mal
lard
) was
pos
itive
for L
PAI s
ubty
pe H
11N
9. In
tota
l the
re w
ere
77
posi
tive
wild
bird
s fo
r LPA
I. W
ithin
the
surv
eilla
nce
of w
ild b
irds
ther
e w
ere
test
ed 7
51 b
irds
in 2
009.
Out
of t
his
num
ber w
ere
test
ed 5
32 b
irds
(mal
lard
s –
4 cl
inic
ally
hea
lth a
nd 5
28
hunt
ed) i
n th
e fr
ame
of a
ctiv
e su
rvei
llanc
e an
d 21
9 bi
rds
(foun
d de
ath)
with
in p
assi
ve s
urve
illan
ce. I
n to
tal 2
5 bi
rds
wer
e po
sitiv
e.
In 2
008,
ther
e w
ere
only
pos
itive
ser
olog
ical
find
ings
with
in th
e su
rvei
llanc
e in
wild
bird
s. In
tota
l 814
wild
bird
s w
ere
exam
ined
from
wha
t pas
sive
su
rvei
llanc
e pr
esen
ts 2
59 w
ild b
irds
(foun
d de
ad) a
nd a
ctiv
e su
rvei
llanc
e 55
5 w
ild b
irds
- mal
lard
(4 c
linic
ally
hea
lthy,
551
hun
ted)
. In
tota
l the
re w
ere
57
Page
26 su
r 32
versio
n : 2
.2
Stan
dard
requ
irem
ents
for t
he su
bmis
sion
of
surv
eilla
nce
prog
ram
mes
for a
vian
influ
enza
sero
logi
cal p
ositi
ve fr
om w
hat f
ive
bird
s w
ere
posi
tive
for L
PAI H
5N3.
H5
and
H7
subt
ypes
wer
e ru
led
out i
n ot
her c
ases
. Sam
ples
wer
e ex
amin
ated
to
conf
irm o
r rul
e ou
t the
pre
senc
e of
viru
s A
I. A
ll re
sults
hav
e be
en n
egat
ive.
W
ithin
the
surv
eilla
nce
in w
ild b
irds,
649
wild
bird
s in
tota
l wer
e ex
amin
ed d
urin
g th
e pe
riod
from
1 Ja
nuar
y 20
07 to
31
Dec
embe
r 200
7 an
d on
e po
sitiv
e vi
rolo
gica
l fin
ding
HPA
I H5N
1 w
as d
etec
ted
in m
ute
swan
in S
outh
-Mor
avia
n Re
gion
(cat
astr
al te
rrito
ry o
f mun
icip
ality
Led
nice
) on
25th
June
200
7.
6.
Mea
sure
s in
plac
e as
rega
rds t
he n
otifi
catio
n of
the
dise
ase
(max
. 320
00 c
hars
) :
The
mea
sure
s in
pla
ce a
re in
acc
orda
nce
with
Cou
ncil
Dire
ctiv
e 20
05/9
4/EC
of 2
0 D
ecem
ber 2
005
on C
omm
unity
mea
sure
s fo
r the
con
trol
of a
vian
in
fluen
za. T
he C
ounc
il D
irect
ive
has
alre
ady
been
impl
emen
ted
in C
zech
legi
slat
ion
trou
gh to
Dec
ree
No.
36/
2007
col
l.
7.
Cost
s
7.1
Det
aile
d an
alys
is o
f the
cos
ts
7.1.
1 P
oultr
y
Page
27 su
r 32
versio
n : 2
.2
Stan
dard
requ
irem
ents
for t
he su
bmis
sion
of
surv
eilla
nce
prog
ram
mes
for a
vian
influ
enza
(max
. 320
00 c
hars
) :
The
over
all e
stim
ated
cos
t of t
he A
I sur
veill
ance
in p
oultr
y du
ring
year
201
3 is
28
093.
50 €
. The
est
imat
ed n
umbe
r of 1
89 p
oultr
y ho
ldin
gs is
to b
e te
sted
in
the
fram
e of
AI s
urve
illan
ce p
rogr
amm
e du
ring
2013
. The
cos
t of t
he p
rogr
amm
e in
clud
ing
the
cost
of a
lum
p su
m o
f 0,5
€ p
er p
oultr
y sa
mpl
ed, l
abor
ator
y te
stin
g by
pre
-scr
eeni
ng s
erol
ogic
al te
sts
and
cost
for f
ollo
win
g te
sts
in c
ase
of p
ositi
ve p
re-s
cree
ning
test
. The
fina
ncia
l con
trib
utio
n by
the
Uni
on s
hall
incl
ude
a lu
mp
sum
of 0
,5 €
per
pou
ltry
sam
pled
and
sha
ll be
at t
he ra
te o
f 60
% o
f the
cos
ts to
be
incu
rred
for t
he c
osts
of c
arry
ing
out l
abor
ator
y te
st.
7.1.
2
Wild
bird
s
(max
. 320
00 c
hars
) :
The
over
all e
stim
ated
cos
t of t
he A
I sur
veill
ance
in w
ild b
irds
durin
g ye
ar 2
013
is 1
9 69
7.30
€. T
he e
stim
ated
num
ber o
f 300
wild
bird
s is
to b
e te
sted
in th
e fr
ame
of A
I sur
veill
ance
pro
gram
me
durin
g 20
13 (p
assi
ve s
urve
illan
ce o
n si
ck a
nd d
ead
wild
bird
s). T
he c
ost o
f the
pro
gram
me
incl
udin
g th
e co
st o
f a
lum
p su
m o
f 5 €
per
wild
bird
sam
pled
and
viro
logi
cal l
abor
ator
y te
stin
g. T
he c
ompe
tent
aut
horit
y w
ishe
s 60
% o
f co-
finan
cing
of t
he c
ost t
o be
incu
rred
fo
r the
cos
ts o
f car
ryin
g ou
t lab
orat
ory
test
s an
d a
lum
p su
m o
f 5 €
per
wild
bird
sam
pled
as
the
finan
cial
con
trib
utio
n of
the
Uni
on.
Page
28 su
r 32
versio
n : 2
.2
Stan
dard
requ
irem
ents
for t
he su
bmis
sion
of
surv
eilla
nce
prog
ram
mes
for a
vian
influ
enza
7.2
Sum
mar
y of
the
cost
s
7.2.
1
Poul
try
surv
eilla
nce
Det
aile
d an
alys
is o
f the
cos
t of t
he p
rogr
amm
e - p
oultr
y
Labo
rato
ry te
stin
g Met
hods
of l
abor
ator
y an
alys
isN
umbe
r of t
ests
Uni
tary
test
cos
t (pe
r met
hod)
in €
Tota
l cos
t (€)
ELIS
A te
st2
530
4.45
1125
8.5
agar
gel
imm
une
diffu
sion
test
00
0
Hae
mag
glut
inat
ion-
inhi
bitio
n-te
st (H
I) fo
r H5
(spe
cify
num
ber o
f tes
ts fo
r H5)
350
4.45
1557
.5
Hae
mag
glut
inat
ion-
inhi
bitio
n-te
st (H
I) fo
r H7
(spe
cify
num
ber o
f tes
ts fo
r H7)
350
4.45
1557
.5
Viru
s iso
latio
n te
st20
56.6
111
32.2
PCR
test
200
40.4
480
88
Sequ
encin
g H
cle
avag
e sit
e20
80.8
716
17.4
Sequ
encin
g N
cle
avag
e sit
e20
80.8
716
17.4
Add
a n
ew ro
w
Page
29 su
r 32
versio
n : 2
.2
Stan
dard
requ
irem
ents
for t
he su
bmis
sion
of
surv
eilla
nce
prog
ram
mes
for a
vian
influ
enza
Sam
plin
g
Num
ber o
f sam
ples
Uni
tary
cos
t in
€To
tal c
ost (
€)
Sam
ples
2 53
00.
512
65
Oth
er m
easu
res
Num
ber o
f sam
ples
Uni
tary
cos
t in
€To
tal c
ost (
€)
Oth
er p
leas
e sp
ecify
her
e0
00
x
Add
a n
ew ro
w
Tota
l pou
ltry
Te
stin
g +
Sam
plin
g +
Oth
er m
easu
res
6 02
028
093
,50
€
Page
30 su
r 32
versio
n : 2
.2
Stan
dard
requ
irem
ents
for t
he su
bmis
sion
of
surv
eilla
nce
prog
ram
mes
for a
vian
influ
enza
7.2.
2
Wild
bird
surv
eilla
nce
Det
ail a
naly
sis o
f the
cos
t of t
he p
rogr
amm
e - w
ild b
irds
Labo
rato
ry te
stin
g Met
hods
of l
abor
ator
y an
alys
isN
umbe
r of t
ests
Uni
tary
test
cos
t (pe
r met
hod)
in €
Tota
l cos
t (€)
Viru
s iso
latio
n te
st50
56.6
128
30.5
PCR
test
300
40.4
412
132
Sequ
encin
g H
cle
avag
e sit
e40
80.8
732
34.8
Sequ
encin
g N
cle
avag
e sit
e40
80.8
732
34.8
Oth
er p
leas
e sp
ecify
her
e0
00
Add
a n
ew ro
w
Sam
plin
g
Num
ber o
f sam
ples
Uni
tary
cos
t in
€To
tal c
ost (
€)
Sam
ples
300
515
00
Page
31 su
r 32
versio
n : 2
.2
Stan
dard
requ
irem
ents
for t
he su
bmis
sion
of
surv
eilla
nce
prog
ram
mes
for a
vian
influ
enza
Oth
er m
easu
res
Num
ber
Uni
tary
cos
t in
€To
tal c
ost (
€)
Oth
er p
leas
e sp
ecify
her
e0
00
x
Add
a n
ew ro
w
Tota
l wild
bird
s T
estin
g +
Sam
plin
g +
Oth
er m
easu
res
690
19 6
97,3
0 €
Gra
nd T
otal
Po
ultry
+ W
ild b
irds
6710
47 7
90,8
0 €
Page
32 su
r 32
versio
n : 2
.2
Stan
dard
requ
irem
ents
for t
he su
bmis
sion
of
surv
eilla
nce
prog
ram
mes
for a
vian
influ
enza
Atta
chm
ents
IMP
OR
TAN
T :
1) T
he m
ore
files
you
atta
ch, t
he lo
nger
it ta
kes
to u
ploa
d th
em .
2)
Thi
s at
tach
men
t file
s sh
ould
hav
e on
e of
the
form
at li
sted
her
e : .
zip,
jpg,
jpeg
, tiff
, tif,
xls
, doc
, bm
p, p
na.
3)
The
tota
l file
siz
e of
the
atta
ched
file
s sh
ould
not
exc
eed
2 50
0Kb
(+- 2
.5 M
b). Y
ou w
ill re
ceiv
e a
mes
sage
whi
le a
ttach
ing
whe
n yo
u try
to lo
ad to
o m
uch.
4)
IT C
AN
TA
KE
SEV
ERA
L M
INU
TES
TO U
PLO
AD
ALL
TH
E A
TTA
CH
ED
FIL
ES
. Don
't in
terr
upt t
he u
ploa
ding
by
clos
ing
the
pdf a
nd w
ait u
ntil
you
have
rece
ived
a
Sub
mis
sion
Num
ber!
5) Z
ip fi
les
cann
ot b
e op
ened
(by
clic
king
on
the
Ope
n bu
tton)
. All
othe
r file
form
ats
can
be o
pene
d.
MAP OF HPAI H5N1 OCCURRENCES IN WILD BIRDS blue – 2006, red – 2007