surgical recovery of porcine embryos - animal science...surgical recovery of porcine embryos by...
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Surgical Recovery of Porcine Embryos
by
Ginger Mills
Dr.Brett White-Research Professor-Animal Science
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Gilt in Heat shows vulva development
Gilt in Heat shows vulva development
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Gilt in Standing Heat, Ears Erect, Body Rigid
Gilt in Standing Heat, Ears Erect, Body Rigid
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• SUPEROVULATION PROCEDURE• Observation of first estrus• Day 16 –Administer 5 ml dose IM PG600(contains
400 IU Serum Gonadotropin(PMSG)+200IU Chorionic(HCG) Induces fertile estrus.
• Seventy-two hours later administer 1ml dose (hCG) human chorionic gonadtropin to induce ovulation.
• AI Breed gilts every 12 hours when in heat.• Surgical recovery of embryos via oviduct or uterus,
depending on the desired stage of development.
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The Pig is Walked up the Ramp into the Squeeze Chute/Administered
6ml TARK (anesthetic inj. cocktail)
The Pig is Walked up the Ramp into the Squeeze Chute/Administered
6ml TARK (anesthetic inj. cocktail)
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The Pig is Rolled From the Squeeze Chute to the Surgery Cart
The Pig is Rolled From the Squeeze Chute to the Surgery Cart
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Administration of 4ml TARK Into the Ear Vein Via a 21 Gauge Butterfly
Cannula
Administration of 4ml TARK Into the Ear Vein Via a 21 Gauge Butterfly
Cannula
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Placement of Endonasal Tubes into theNares of the Pig
Placement of Endonasal Tubes into theNares of the Pig
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Removal of Body Hair From the Surgical Site
Removal of Body Hair From the Surgical Site
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Betadine Scrubs to prepare surgical site/Leg rope restraint
Betadine Scrubs to prepare surgical site/Leg rope restraint
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Final Hair Removal with RazorFinal Hair Removal with Razor
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Cleanse Surgical Site with 70% Alcohol
Cleanse Surgical Site with 70% Alcohol
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Hand Scrub prior to surgeryHand Scrub prior to surgery
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Flushing Media is removed from incubator and poured into syringeFlushing Media is removed from incubator and poured into syringe
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Surgery to Collect Early Stage Embryos
Surgery to Collect Early Stage Embryos
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Pig is Maintained with Halothane Anesthesia During the Surgery
Pig is Maintained with Halothane Anesthesia During the Surgery
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Incision is made on abdomen of pig between umbilicus and last pair of
teats
Incision is made on abdomen of pig between umbilicus and last pair of
teats
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Mid-Lateral 2-3”Incision Puncturing Peritoneum to Expose the Uterus or
Oviduct
Mid-Lateral 2-3”Incision Puncturing Peritoneum to Expose the Uterus or
Oviduct
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Expose one horn of the uterus Passage of Cannula into the Fimbria
Expose one horn of the uterus Passage of Cannula into the Fimbria
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Insertion of Oviduct Flushing Cannulainto Infundibulum of the Oviduct
Insertion of Oviduct Flushing Cannulainto Infundibulum of the Oviduct
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Cannula must be into the infundibulum to ensure complete
recovery of embryos
Cannula must be into the infundibulum to ensure complete
recovery of embryos
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The Cannula is Held into a 50 ml Tube that will Collect the Flushing
Media Containing the Embryos
The Cannula is Held into a 50 ml Tube that will Collect the Flushing
Media Containing the Embryos
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Penetrate uterine lumen and move through utero-tubular junction and
into the oviduct with blunt-end needle
Penetrate uterine lumen and move through utero-tubular junction and
into the oviduct with blunt-end needle
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Blunt-end needle administering media into the oviduct
Blunt-end needle administering media into the oviduct
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15 mls of Flushing Media is Administered the First Few ML
contain the embryos
15 mls of Flushing Media is Administered the First Few ML
contain the embryos
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Ovary and numerous corpus luteum,the bloody appearance indicates rupture of the
CL at ovulation and release of an egg.
Ovary and numerous corpus luteum,the bloody appearance indicates rupture of the
CL at ovulation and release of an egg.
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Skin Suture to Close Surgical SiteSkin Suture to Close Surgical Site
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n=2018 gilts cycled
Avg – 33 Corpus Luteum/gilt81% Oviductal Recovery
88% Uterine Recovery (n=2)50% made it to surgery
(Superovulated)
n=2018 gilts cycled
Avg – 33 Corpus Luteum/gilt81% Oviductal Recovery
88% Uterine Recovery (n=2)50% made it to surgery
(Superovulated)
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Surgical Embryo RecoveryThe End
Surgical Embryo RecoveryThe End