SureSeq™ NGS Library Preparation KitStreamlined library preparation for industry-leading next generation sequencing(NGS) results

The SureSeq NGS Library Preparation Kit generates NGS libraries suitable for the capture of

targeted genomic regions using hybridisation. Optimisation of enzymes and buffers enables the

number of steps to be reduced, while still delivering libraries of the highest quality.

• Greater trust in your data — High performance with low

duplication rates, high sequence quality and high

percentage of on-target bases

• Faster process — Streamlined protocol reduces manual

handling steps to save time, increasing reliability as well

as throughput

• Reliable results — NGS targeted panel, NGS library

preparation and powerful NGS analysis software are

fully optimised for perfect results every time

Greater trust in your dataNGS data is increasingly being relied upon as a front line technology forthe generation of data for scientific and medical research. A combinationof quality metrics are used to give confidence that variants called are realand the number of false positive calls are low. The SureSeq NGS LibraryPreparation Kit delivers high performance in the quality metrics thatreally matter, giving more reliable, more trustworthy data.

Low levels of duplicationHigh duplication rates reduce the complexity of your NGS library and canlead to poor coverage of target genomic regions even though averagecoverage rates appear high.

The SureSeq NGS Library Preparation Kit gives exceptionally low levels ofduplication (Figure 1). This ensures more accurate calling, more evencoverage and higher levels of confidence in the data produced.

High quality sequence dataFor complete confidence in your data, the sequence quality scores should behigh and consistent across the length of each read. The SureSeq NGS LibraryPreparation Kit delivers exceptional sequence quality scores even 100 basesinto the read (Figure 2). This reduces the requirement for post-acquisitionbioinformatics, such as read size reduction, to remove poor qualitysequence, giving you complete confidence in your data.

Figure 1: The SureSeq NGS Library PreparationKit delivers low levels of sequence duplication.The duplication rates are shown for 8 samplessequenced using exome capture (AgilentSureSelect Human All Exon V5) with the SureSeqNGS Library Preparation Kit and a librarypreparation kit from an alternative supplier.

Figure 2: The SureSeq NGS Library PreparationKit delivers high sequence quality. Data shownwas generated using human genomic DNA, theOGT SureSeq Myeloid Panel and SureSeq NGSLibrary Preparation Kit

SureSeq: For Research Use Only; Not for Diagnostic Procedures.

Figure 3: High percentage of on-target bases.The percentage of on-target bases are shownfor exome capture (Agilent SureSelect HumanAll Exon V5) using the SureSeq NGS LibraryPreparation Kit and a library preparation kitfrom an alternative supplier.

Figure 4: A streamlined protocol, includingenrichment by hybridisation. The completeprocedure can be completed in 1.5 days withminimal handling time.

DNA shearing

End repair & 3’ A-tailing

Adaptor ligation

Pre-capture PCR 1

Design specific baits Purification

Purification

Hybridisation wash

Purification

Purification

Hybridisation

Post-capture PCR 2

Sample pooling & sequencing

Genomic DNA

Product Contents Cat. No.

SureSeq NGS Library Preparation Adaptors, PCR primers and enzymes sufficient for 500070Kit (16 reactions) 16 samples – Index Collection A included

SureSeq NGS Index Kit - Collection A (included 16 different indexes, each sufficient for 4 samples 500071with SureSeq NGS Library Preparation kit)

SureSeq Ovarian Cancer Panel 16 reactions Enrichment baits sufficient for 16 samples; 600073SureSeq Interpret Software

SureSeq Ovarian Cancer Panel 96 reactions Enrichment baits sufficient for 16 samples; 600074SureSeq Interpret Software

SureSeq Myeloid Panel 16 reactions Enrichment baits sufficient for 16 samples; 600075SureSeq Interpret Software

SureSeq Myeloid Panel 96 reactions Enrichment baits sufficient for 16 samples; 600076SureSeq Interpret Software

Oxford Gene Technology, Begbroke Science Park, Woodstock Road, Begbroke, Oxfordshire, OX5 1PF, UKT: +44(0)1865 856826 (US: 914-467-5285) E: products@ogt.com W: www.ogt.com

SureSeq Solid Tumour Panel and SureSeq Interpret Software: For Research Use Only; Not for Diagnostic Procedures. This document and its contents are © Oxford GeneTechnology IP Limited – 2015. All rights reserved. OGT™ and SureSeq™ are trademarks of Oxford Gene Technology IP Limited. The SureSeq NGS Library Preparation Kitwas jointly developed between Oxford Gene Technology and Bioline Reagents Limited.

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High levels of on-target basesIn order to maximize the amount of useful information that can beobtained for a given sequencing lane, high levels of on-target bases arerequired. The benefits of superior levels of specific sequence captureinclude, increased sequence complexity and greater depth of coverageof the target regions. The SureSeq NGS Library Preparation Kit delivers ahigh percentage of on-target bases compared to other librarypreparation kits (Figure 3).

Faster process with streamlined protocol By extensive testing of enzymes and buffer optimisation, it has beenpossible to streamline the standard library preparation protocol to reducethe number of hands-on steps and the overall processing time (Figure 4).

• End repair and ligation are combined into a single step

• Purification steps, where material is lost, are reduced

• Fewer steps reduce handling errors for increased reliability

• Reduced technician cost and increased sample throughput

Reliable results with full optimisation with SureSeqtargeted panels and analysis software The SureSeq NGS Library Preparation Kit works hand-in-hand with ourtargeted enrichment panels, which have been developed with world-leaders in their respective fields. This ensures you get the most sensitiveand reproducible variant detection and industry-leading coverageuniformity.

SureSeq Interpret Software — OGT’s powerful, standalone data analysisand reporting package — is provided free with all our SureSeq targetedpanels to convert FASTQ files into an intuitive interactive report. Variantsare fully annotated with links to various databases (e.g. dbSNP, COSMIC,Genecards and OMIM) providing results in context.

For more information about the SureSeq NGS Library PreparationKit, or any SureSeq NGS product, visit www.ogt.com or contactus at products@ogt.com

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