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Supporting Online Material for

Candidatus Chloracidobacterium thermophilum: An Aerobic Phototrophic

Acidobacterium

Donald A. Bryant,* Amaya M. Garcia Costas, Julia A. Maresca, Aline Gomez Maqueo Chew, Christian G. Klatt, Mary M. Bateson, Luke J. Tallon, Jessica Hostetler, William C.

Nelson, John F. Heidelberg, David M. Ward

*To whom correspondence should be addressed. E-mail: [email protected]

Published 27 July 2007, Science 317, 523 (2007) DOI: 10.1126/science.1143236

This PDF file includes:

Materials and Methods SOM Text Figs. S1 to S13 Table S1 References

1

Supporting Online Material for

A phototrophic acidobacterium discovered by metagenomics

D. A. Bryant*, A. M. Garcia Costas, J. A. Maresca, A. Gomez Maqueo Chew, C. Klatt, M. M.

Bateson, L. J. Tallon, J. Hostetler, W. C. Nelson, J. F. Heidelberg, and D. M. Ward

*To whom correspondence should be addressed. E-mail: [email protected]

This supplement contains:

Materials and Methods

SOM text

Figs. S1 to S13

Table S1

References

2

Supporting Online Material

MATERIALS AND METHODS

Library construction, DNA sequencing, and sequence analysis

The sequence of a representative Roseiflexus sp. isolate, strain RS1 (1-3) was determined

as part of a Joint Genome Institute-Department of Energy project to determine genome

sequences of filamentous anoxygenic phototrophs (http://genome.jgi-psf.org/; GenBank

accession AAQU00000000). The Synechococcus sp. OS-A and OS-B' genome sequences (4)

(GenBank accessions CP000239 and CP000240) and metagenomic clone libraries and databases

(4-6) used in this study were developed for an NSF Frontiers in Integrative Biology Research

project (7). Amplicon Express (Pullman, WA) constructed the bacterial artificial chromosome

(BAC) clone library. Sequence data derived from BAC clone M60-018 J19 (see Fig. 6S) have

been deposited in GenBank under accession number EF531339.

Sequencing and assembly of the metagenome were performed as described for genomes

sequenced by TIGR and the J. Craig Venter Institute (8). BAC closure was done by mechanical

shearing of the entire BAC followed by random shotgun sequencing on small insert (2-3 kbp)

clones. The plasmid sequences were jointly assembled using the TIGR Assembler (9). The

coverage criteria were that every position required at least double-clone coverage (or sequence

from a PCR product amplified from genomic DNA), and either sequence from both strands or

with two different sequencing chemistries. The sequence was edited manually, and additional

PCR (10) and sequencing reactions were performed to close gaps, improve coverage, and resolve

sequence ambiguities.

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An initial set of open reading frames likely to encode proteins (coding sequence, CDS)

were predicted for the BAC insert by Glimmer (11). All predicted proteins larger than 30 amino

acids were searched against a non-redundant protein database as previously described (12-14).

TopPred was used to predict the membrane-spanning domains of proteins (15). The 5' regions of

each CDS were inspected to define initiation codons using homologies, and position of

ribosomal binding sites and transcriptional terminators. Two sets of hidden Markov models were

used to determine CDS membership in families and superfamilies: pfam v11.0 (16) and

TIGRFAMs 3.0 (17). Pfam v11.0 hidden Markov models were also used with a constraint of a

minimum of two hits to find and mask repeated domains within proteins.

Database searches were performed using the various blast algorithms to search the

GenBank non-redundant databases (18, 19). For construction of phylogenetic trees, protein

sequences were aligned using the ClustalW tool in MacVector versions 7.2.3 or 9.0.2 (Accelrys,

San Diego, CA). Neighbor-joining trees for type-1 reaction centers and BchU were generated

from the alignments using PAUP 4.0 (Sinauer Associates, Inc., Sunderland, MA). The neighbor-

joining tree for RecA was generated using MacVector version 9.0.2 (Accelrys, San Diego, CA).

Enrichment culture methods and isolation of Anoxybacillus sp.

Enrichment cultures containing Synechococcus sp. strain JA-2-3B'a (2-13),

Chloracidobacterium (Cab.) thermophilum, and Anoxybacillus sp. (20) were grown in B-HEPES

medium at pH 8.0 (21) at 45-53°C. In cultures to maintain and enrich for Cab. thermophilum, the

B-HEPES medium was supplemented with 1 mM ammonium chloride and a mixture of carbon

sources: lactate, succinate, glycolate, butyrate, propionate, and acetate (1 mM each, added in the

form of sodium salts). Nitrate was omitted from the growth medium of cultures that did not

contain cyanobacteria. The medium was also supplemented with filter-sterilized vitamins at the

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following final concentrations: (10 µg L-1) riboflavin, vitamin B12 (100 µg L-1), thiamine-HCl

(100 µg L-1), L-ascorbic acid (100 µg L-1), D-Ca-panthothenate (100 µg L-1), folic acid (100 µg

L-1), nicotinic acid (100 µg L-1), 4-aminobenzoic acid (100 µg L-1), pyridoxine-HCl (100 µg L-1),

and lipoic acid (100 µg L-1). To inhibit the growth of the cyanobacterial cells in the original

enrichment, atrazine was added to a final concentration of 10 µM.

Unless otherwise specified, all cultures were incubated under oxic conditions with or

without shaking in constant light provided by a 60 W tungsten and a 60 W fluorescent bulb (total

visible light intensity of 67 µmol photons m-2 s-1). Synechococcus sp. strain JA-2-3B'a (2-13) was

eliminated from an enrichment that also contained Anoxybacillus sp. and Cab. thermophilum by

serial subculturing three times in the light in the presence of 10 µM atrazine. Anoxybacillus sp.

was isolated in axenic culture by plating cells from the enrichment cultures on Luria-Bertani

medium solidified with 1.5% (w/v) Difco Bacto-Agar (Becton-Dickinson, Franklin Lakes, NJ).

Anoxybacillus sp. was identified by amplifying and DNA sequencing a region of its 16S rRNA

by using universal bacterial primers (Table 1S).

Light microscopy

Cab. thermophilum cells were incubated in 1-hexanol-saturated phosphate-buffered

saline to disrupt bacteriochlorophyll (BChl) c aggregates (22) and visualized in an Olympus BX-

60 epifluorescence upright microscope equipped with an UplandFL 100× oil objective, a

mercury vapor lamp and Olympus filter cubes 41001 (Olympus, Center Valley, PA).

Absorption and fluorescence emission spectra

5

Absorption spectra were either recorded with a Cary 14 UV-Vis-NIR spectrophotometer

modified for computerized data acquistion by On-Line Systems, Inc. (Bogart, GA) or with a

GENESYS 10 spectrophotometer (Thermo Electron Corp., Rochester, NY). Data were analyzed

using Igor-Pro software (Wavemetrics, Inc. Lake Oswego, OR).

Chlorosome isolation and electron microscopy

Chlorosomes were isolated from fresh cells (2 L) as described by Vassilieva et al. (23).

Isolated chlorosomes were negatively stained with 1% (w/v) uranyl acetate and visualized in a

JEOL 1200 EXII Transmission electron microscope (Peabody , MA).

Pigment extraction and analysis

Pigments were extracted from 1-ml aliquots of cell suspension from late-exponential

phase enrichment cultures by sonication in acetone-methanol (7:2, v/v). Cell debris was removed

by centrifugation. The absorption spectrum of the pigment mixture was recorded after diluting

the sample at least 1:10 in methanol, and the absorption spectrum of the extracts was recorded

from 350 to 900 nm. Samples for HPLC analysis were filtered and 0.1 volume of 1 M

ammonium acetate was added to the filtrate immediately before injection into the HPLC column.

The pigments were separated on a 25 cm by 4.6 mm Discovery 5 µm C18 column (Supelco,

Bellefonte, PA) attached to a 1,024-element diode array detector (model G1315B, 1100 series;

Agilent Technologies, Palo Alto, CA) controlled with Agilent ChemStation software for HPLC.

The solvent system was previously described (24). BChl c and BChl a extracted from Chl.

tepidum and from Cfx. aurantiacus Y-400-fl served as standards (see Fig. 4). Elution of the

pigments was monitored at 667 and 770 nm, the absorbance maxima for BChl c and BChl a

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respectively; the absorption spectrum of the column eluate was recorded from 200 to 900 nm at

two-second intervals.

Polymerase chain reaction and agarose gel electrophoresis

In general, the polymerase chain reaction conditions consisted of 5 minutes at 95oC, 35

cycles of 45 seconds at 95oC, 45 seconds at 57oC and 1 minute at 72oC, followed by a final

elongation step of 10 minutes at 72oC. About 10-100 ng of DNA were included in each reaction

and primers (see Table 1S) were supplied at a final concentration of 1 µM. PCR products were

electrophoresed in a 0.8% (w/v) agarose gel and stained with ethidium bromide prior to

visualization.

SUPPORTING ON-LINE MATERIAL

History of discovery of photosynthetic bacteria and their differentiating characteristics

Five bacterial phyla (Cyanobacteria, Chlorobi, Proteobacteria, Chloroflexi, and

Firmicutes) contain members, chlorophototrophs, capable of phototrophy based upon BChl or

chlorophyll (Chl) (25). (Throughout this article, we have used the terms phylum and phyla as

employed in the Second Edition of Bergey’s Manual of Systematic Bacteriology (26), although

there is debate as to whether the major sublineages of Domains should be considered kingdoms,

as in the case of plants, animals, and fungi.). Studies of Cyanobacteria and photosynthetic

Proteobacteria (i.e. the purple bacteria) date to the first half of the 19th century, and green sulfur

bacteria (Chlorobi) were first described in 1906 (26, 27). Cyanobacteria perform oxygenic

photosynthesis by virtue of their Chl a-containing, type-1 (photosystem I) and type-2

(photosystem II) reaction centers, and they fix carbon by the reductive pentose-phosphate

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(Calvin-Benson-Bassham) cycle. Endosymbiosis of a cyanobacterium led to the acquisition of

oxygenic photosynthesis by eukaryotic algae and plants. The purple chlorophototrophic bacteria,

all members of the alpha, beta, and gamma subdivisions of the Proteobacteria, have relatively

simple, type-2 reaction centers with either BChl a or BChl b, do not evolve oxygen, and also fix

carbon via the reductive pentose phosphate pathway, although most can grow mixotrophically

when presented with light and a usable organic carbon source. Many purple bacteria are aerobic

chemoheterotrophs under oxic conditions, but are only facultatively phototrophic under anoxic

conditions. Chlorobi are strictly anaerobic, photoautotrophic organisms that have type-1 reaction

centers with chlorosomes as antennae; any given strain can produce three types of Chl (Chl a,

BChl a, and either BChl c, d, or e) and fix CO2 by the reverse TCA cycle.

In the 100 years since the initial description of the Chlorobi (27), only two additional

bacterial phyla have been shown to contain members that can produce BChls and reaction

centers. Pierson and Castenholz (28) isolated the filamentous anoxygenic phototroph,

Chloroflexus aurantiacus, from the same microbial mats of Octopus Spring and other thermal

features around the world. This moderate thermophile synthesizes BChls a and c, produces

chlorosomes as light-harvesting antennae, has type-2 reaction centers similar to those of purple

bacteria, and fixes CO2 by the 3-hydroxypropionate cycle. Cfx. aurantiacus is the type species of

the Chloroflexi, an early-diverging phylum of the Bacteria. Strains of both major subdivisions of

the Chloroflexi contain the genes required for carbon fixation by the 3-hydroxypropionate cycle

(2), although it should be noted that some chlorophototrophic Chloroflexi possess Rubisco and

fix carbon by the reductive pentose-phosphate pathway (29). The most recently discovered

reaction center-containing bacteria are the heliobacteria, which were originally described by Gest

and Favinger in 1983 (30). Heliobacteria are Gram-positive bacteria, i.e., Firmicutes, which form

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heat-resistant endospores and that are closely related to clostridia (see Fig. 8S). These strict

anaerobes produce type-1 reaction centers containing BChl g, but no known isolate can grow

photoautotrophically.

Sequence analysis of a pscAB-fmoA operon recovered from the metagenome of the

phototrophic mat community of alkaline siliceous hot springs

Inserts encoding the highly divergent pscA gene of an organism from the phototrophic

layer of the microbial mats of Octopus and Mushroom Springs in Yellowstone NP were

completely sequenced from four independent plasmids and one BAC insert (see below; GenBank

accession EF531339). The overlapping data revealed a probable operon, 5’pscAB-fmoA,

encoding the type-1 reaction center (PscAB) and the FmoA apoprotein of the BChl a-binding

Fenna-Matthews-Olson protein (Fig. 1B, Fig. 6S). Preliminary phylogenetic analyses of the

flanking genes suggested that these DNA sequences were derived from an organism related to

Acidobacterium sp. Ellin345 or Solibacter usitatus Ellin6076, two soil bacteria belonging to the

poorly characterized phylum Acidobacteria (26), whose genomes have recently been sequenced

(3).

The three sequences deduced from the pscAB-fmoA gene cluster were divergent from

known homologs in other anoxygenic chlorophototrophs. PscA was predicted to be a polypeptide

of 865 amino acids (aa), which was ~135 aa larger than the PscA subunits of green sulfur

bacterial type-1 reaction centers, to which it was most similar (31) (Fig. 1A, 1S). This reaction

center apoprotein was more distantly related to PshA (~610 aa) of heliobacteria and the PsaA

and PsaB subunits (730-750 aa) of cyanobacterial photosystem I (Figs. 1, 1S-3S). Like the

apoproteins of all other type-1 reaction centers, the mat PscA was predicted to contain eleven,

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transmembrane α-helices. An insertion of ~165 aa between predicted transmembrane α-helices

VII and VIII accounted for most of the size difference (Fig. 1B, 1S). This insertion corresponded

to a large extrinsic loop, which might form a docking site for a periplasmic electron donor to the

oxidized special pair. The C-terminal electron transfer domain of PscA, comprising the last four

transmembrane α-helices of the protein, was most similar to the same domain of green sulfur

bacterial PscA sequences (Figs. 1, 3S), but this domain was nevertheless quite divergent (48%

identical, 69% similar). However, the highly conserved motif, FPCIGPVKGGTCGVS,

containing two cysteine residues in the cytoplasmic loop between transmembrane helices VIII

and IX (Fig. 3S), implied that this reaction center contains an intersubunit [4Fe-4S] cluster

similar to FX of PS I (31). The N-terminal, antenna domain of this PscA-like protein is not well

conserved and was difficult to align to other reaction center sequences (Fig. 2S). It exhibits only

~29% identity and ~43% similarity to the N-terminal antenna domain of PscA from green sulfur

bacteria.

Similar to PscB of green sulfur bacterial Type-1 reaction centers (31) and the PsaC

protein of photosystem I (32, 33), the mat-derived PscB was predicted to be a small protein with

eight conserved cysteines, which should ligate two terminal, electron-accepting, [4Fe-4S]

clusters (see Fig. 4S). The region encompassing these cysteines was well conserved, but the

remainder of the protein was much less similar to PscB sequences of green sulfur bacteria. The

fmoA gene encodes the Fenna-Matthews-Olson protein, a BChl a-binding protein that occurs in

all green sulfur bacteria (34). The fmoA gene is only known to occur in green sulfur bacteria, but

in 12 sequenced green sulfur bacterial strains this gene is unlinked to pscAB operons. Although

the FmoA proteins of green sulfur bacteria are highly similar in sequence (~70% identity, ~85%

similarity), the pscAB-linked fmoA gene encoded a protein that is only ~40% identical and 58%

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similar to FmoA sequences of green sulfur bacteria (Fig. 5S). Based upon these data, we

hypothesized that a thermophilic acidobacterium with a novel type-1 reaction center and FmoA

occurs in the mats.

Identification and sequencing of a bacterial artificial chromosome insert encoding pscAB-

fmoA, recA, and the rDNA operon of an acidobacterium

To compile more information about the putative acidobacterium with type-1 reaction

centers, metagenomic reads from ~60°C and ~65°C sites of the Octopus and Mushroom Spring

microbial mats were assembled to produce consensus contigs. Most of the biomass in these mats

belongs to organisms closely related to Synechococcus sp. OS-A and OS-B' and Roseiflexus sp.

RS1 (35, 36), whose genomes have been sequenced (2-4, 37). Sequences showing >92% identity

over 80% of the read to these three organisms were first removed. Contigs with G+C contents of

~56 mol% and with multiple gene products showing best hits from blastx analyses to

acidobacteria and/or δ-proteobacteria were then binned as possibly being derived from a single

type of mat bacterium. This bin contained two scaffolds that encoded well-established

phylogenetic markers: a complete recA gene and a partial 16S rRNA gene. Bacterial artificial

chromosome (BAC) clones, constructed from DNA isolated from the photic-zone mat layer of

the ~60°C Mushroom Spring site, were end-sequenced and mapped onto the binned scaffolds

(Fig. 6S). These analyses predicted that the binned pscAB-fmoA, recA, and the rRNA genes were

encoded on a single BAC insert. Sequencing of this 271,846 bp BAC insert (Fig. 6S) confirmed

that the pscAB-fmoA and rRNA operons were separated by ~183 kbp with the recA gene between

them. The complete 16S rRNA sequence derived from the BAC clone is identical to the partial

sequence assembled from the metagenome and is nearly identical (differences at 2 of 1348

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shared positions) to that from an acidobacterium, denoted GFP1, the 16S rRNA sequence of

which was recovered from Green Finger Pool in the Lower Geyser Basin of Yellowstone NP (a

site ~5 km from Octopus Spring) (see Fig. 1 of 38) (Fig. 7S). These data unequivocally establish

that the highly divergent pscAB-fmoA genes are derived from a GFP1-like organism belonging to

the phylum Acidobacteria. Phylogenetic analyses of RecA are consistent with this conclusion

(Fig. 8S, 9S).

Analyses of sequences tentatively assigned to the GFP1-like acidobacterium.

The binned sequence assemblies putatively derived from the GFP1-like acidobacterium

were annotated by computer and analyzed. The acidobacterial sequence bin contained several

genes for BChl and carotenoid biosynthesis as well as genes associated with chlorosomes. For

example, bchU (Fig. 10S, 11S), whose product is a C-20 methyltransferase required for the

synthesis of BChl c of green bacteria (39) and bchK, whose product is the BChl c synthase (40),

were found. A radical-SAM-type methyltransferase similar to BchR, which methylates C-121 of

BChl c (41), was also identified. These data predicted that the acidobacterium should produce

methylated Bchl c homologs and assemble chlorosomes like green sulfur bacteria. Consistent

with this inference, a csmA gene, whose product is a BChl a-binding protein of the chlorosome

baseplate (42), was also found in a putative operon with bchG, encoding a BChl a synthase (see

Fig. 6S). Overlapping metagenome scaffolds and BAC clones physically linked these genes and

bchY, a subunit of chlorophyllide a reductase, to the genes on the sequenced BAC insert (Fig.

6S). No oxygen-independent magnesium-protoporphyrin IX monomethyl ester oxidative cyclase

(bchE) was identified. However, the presence of acsF, encoding an oxygen-dependent

magnesium-protoporphyrin IX monomethyl ester oxidative cyclase (41, 43, 44), implied that the

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mat acidobacterium should synthesize BChl under oxic conditions (Fig. 12S). Genes with strong

sequence similarity to crtB, crtP, and crtH further imply that the GFP1-like organism synthesizes

carotenoids by a pathway similar to that in Chlorobi and Cyanobacteria (45). Although an mcr

gene encoding malonyl-CoA reductase, an enzyme required for CO2 fixation by the 3-

hydroxypropionate pathway (2), was identified, no other genes specifically attributable to CO2

fixation were identified. No genes encoding hydrogenases or enzymes for sulfide/sulfur

oxidation were detected.

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Supplemental Figure Legends

Figure 1S. Diagram comparing the relative positions of transmembrane α-helices and loops of

various Type-1 reaction center polypeptides. Bars connecting the tops of the rectangles represent

loops in the cytoplasm and bars connecting the bottoms of the rectangles represent loops in the

periplasm or thylakoid lumen. Note the very large periplasmic loop between helices VII and VIII

of the Cab. thermophilum. The positions of the FX Cys ligands and the conserved histidine ligand

to the reaction center special pair (P700, P840, P780) are indicated. The antenna (helices I to VI)

and electron-transfer domains (helices VII to XI) of the polypeptides are indicated.

Abbreviations and sequence alignments of selected Type-1 reaction center proteins can be found

in Figures 2S and 3S.

Figure 2S. Sequence alignment of the N-terminal region of PscA of Cab. thermophilum and the

homologous PscA, PshA, PsaA, and PsaB proteins of the Type-1 reaction centers and other

photosynthetic bacteria. The region shown corresponds to that including transmembrane α–

helices I through VII, including all of the antenna domain and the first transmembrane helix of

the electron transfer domain; the positions of these helices for Thermosynechococcus elongatus,

as derived from the X-ray structure for photosystem I (47), are indicated. Note that the long

inserted loop sequence that follows helix VII of Cab. thermophilum has been truncated, since it

is not related in sequence to any portion of any other reaction center protein. Hyphens indicate

insertions/deletions included to optimize the alignment. Abbreviations: Cab.,

Chloracidobacterium; Chl., Chlorobium; Hbc., Heliobacillus; Hbt., Heliobacterium; Pld.,

Pelodictyon; Ptc., Prosthecochloris; T., Thermosynechococcus.

14

Figure 3S. Sequence alignment of the C-terminal region of PscA and the homologous PshA,

PsaA, and PsaB proteins of the reaction centers of Cab. thermophilum and other photosynthetic

bacteria. The region shown corresponds to transmembrane α–helices VIII through XI, including

most of the electron-transfer cofactor binding domain; the positions of these helices for

Thermosynechococcus elongatus as derived from the X-ray structure for photosystem I (47) are

shown. Hyphens indicate insertions/deletions included to optimize the alignment. The position of

the conserved, cysteine-containing loop (red boxes enclose the conserved cysteine residues) that

is predicted to provide the ligands to an intersubunit FX-like, [4Fe-4S] cluster is indicated by the

bar labeled FX. The conserved His in Helix X that is the putative ligand to one BChl/Chl of the

reaction center special pair is also indicated by a red box. Abbreviations are the same as in

Figure 3S.

Figure 4S. Sequence alignment of PscB sequences from various organisms. Hyphens indicate

insertions/deletions included to optimize the alignment. Abbreviations: Cab.,

Chloracidobacterium; Chl., Chlorobium; Ptc., Prosthecochloris. The eight conserved cysteines

that are likely to be the ligands to two [4Fe-4S] clusters are indicated in red boxes and in the

consensus sequence.

Figure 5S. Sequence alignment of selected FmoA proteins, the apoprotein of the BChl a-binding

Fenna-Matthews-Olson protein. Hyphens indicate insertions/deletions included to optimize the

alignment. Abbreviations: Cab., Chloracidobacterium; Chl., Chlorobium; and Ptc.,

Prosthecochloris.

15

Figure 6S. Physical map of a 950-kbp region of the consensus Cab. thermophilum genome,

including BAC clone M60-018 J19 and showing the relative positions of the rRNA operon, recA,

pscA-pscB-fmoA, bchG-orf-csmA, and bchY genes. A. Physical map of assembled scaffolds and

contigs from the Mushroom Spring metagenome (dark blue bars) mapped onto the sequence of

BAC clone M60-018 J19 (blue-green bar). This BAC clone is completely sequenced and

comprises 271,846 bp (GenBank accession EF531339). The colored boxes indicate the positions

of the 16S rRNA (purple), recA (red), and pscAB-fmoA (green) genes. B. Extension of the map in

Panel A that shows the physical map of sequenced BAC clone M60-018 J19 (blue-green bar) and

other BAC clones (gray bars) whose end-sequences (pink boxes) were mapped onto the scaffolds

and contigs (dark blue bars) assembled from the Mushroom and Octopus Springs metagenome.

The positions of the bchG-orf-csmA (yellow-green) and bchY (orange) genes are indicated,

establishing that these genes are also physically linked to the 16S rRNA gene in BAC clone

M60-018-J19.

Figure 7S. Comparison of the complete sequence of the 16S rRNA of Cab. thermophilum

derived from BAC clone M60-018 J19 with the nearly complete sequence from Acidobacterium

GFP1 (37). The red ellipses indicate the differences between the two sequences.

Figure 8S. Rooted neighbor-joining phylogram based upon the sequence alignment shown in

Figure 9S, which shows the relationships of RecA sequences of Cab. thermophilum and various

other organisms. The RadA-like protein (DR1105) of Deinococcus radiodurans was used as the

outgroup sequence. Some of the major eubacterial phyla are listed at the right.

Chlorophototrophs and the phyla that contain them are shown in colors, and all other phyla and

16

organisms are indicated in black. Bootstrap values were determined on the basis of 2000

resamplings.

Figure 9S. Alignment of the RecA sequences from the indicated organisms that was used to

generate the tree shown in Figure 8S. Hypens indicate insertions/delections included to optimize

the alignment. Abbreviations: Cab., Chloracidobacterium; Car., Carboxydothermus; Cfx.,

Chloroflexus; Hb., Heliobacterium; Pel., Pelotomaculum; Pld., Pelodictyon; The.,

Thermoanaerobacter.

Figure 10S. Rooted neighbor-joining phylogram derived from the sequence alignment shown in

Figure 11S showing the relationship of the BchU sequence of Cab. thermophilum to the BchU

and CrtF sequences of various organisms. The ComT methyltransferase of Ara. thaliana was

used as the outgroup. Abbreviations: A., Arabidopsis; Cab., Chloracidobacterium; Cfx.,

Chloroflexus; Chl., Chlorobium; Pld., Pelodictyon; Ptc., Prosthecochloris; Rhb., Rhodobacter;

Rps., Rhodopseudomonas; Rvx., Rubrivivax. The bootstrap values shown are based on 1000

resamplings.

Figure 11S. Alignment of BchU, CrtF, and ComT sequences from the indicated organisms that

was used to generate the tree shown in Figure 10S. Hyphens indicate insertions/deletions

included to optimize the alignment. Abbreviations: A., Arabidopsis; Cab., Chloroacidobacterium;

Cfx., Chloroflexus; Chl., Chlorobium; Pld., Pelodictyon; Ptc., Prosthecochloris; Rhb.,

Rhodobacter; Rps., Rhodopseudomonas; Rvx., Rubrivivax.

17

Figure 12S. Alignment of sequences for AcsF, the oxygen-dependent oxidative ring cyclase for

Mg-protoporphyrin IX monomethyl ester in BChl and Chl biosynthesis (41, 43, 44). Hyphens

indicate insertions/deletions included to optimize the alignment. Abbreviations: A., Arabidopsis;

Cab., Chloracidobacterium; Cfx., Chloroflexus; G., Gloeobacter; Por., Porphyra; Rb.,

Rhodobacter; Rfx., Roseiflexus; Rps., Rhodopseudomonas.

Figure 13S. Complete HPLC elution profile monitored at 667 nm for pigments extracted from

an enrichment culture containing Synechococcus sp. strain JA-2-3B'a (2-13), Cab. thermophilum,

and Anoxybacillus sp. The blue-colored block indicates the elution positions of BChl c homologs

esterified with farnesyl (indicated by the subscript “F”). The yellow colored block indicates the

elution position of BChl c homologs esterified with geranylgeraniol (indicated by the subscript

“GG”). Most of the BChl c is esterified with alcohols more hydrophobic than geranylgeraniol

(aqua colored block), and at least two distinct alcohol groups appear to be present because of the

complexity of the pattern observed. The peaks numbered 1 to 4 indicate the typical pattern of

[ethyl, methyl], [ethyl, ethyl], [propyl, ethyl] and [i-butyl, ethyl] BChl c homologs, and the

letters “a” and “b” indicate peaks probably derived from two different esterifying alcohol groups.

Peaks from Chl a of Synechococcus sp. strain JA-2-3B'a (2-13) are indicated by the two arrows.

The inset shows the spectrum of peak 3b, which is characteristic of BChl c under these solvent

conditions.

Synechococcus sp. 7002 PsaA

Synechococcus sp. 7002 PsaB

Chl. tepidum PscA

Hbt. modesticaldum PshA

Cab. thermophilum PscA

865 aa

608 aa

739 aa

733 aa

731 aa

Electron transfer domainAntenna domain

FX

FX

FX

FX

FX

P700

P700

P840

P840

P780

Figure 1S

= Transmembrane alpha-helix

PscA/PshA/PsaA/PsaB/ N-terminal Alignments

T. elongatus PsaBSynechococcus 7002 PsaBT. elongatus PsaASynechococcus 7002 PsaAHbc. mobilis PshAHbt. gestii PshAHbt. modesticaldum PshACab. thermophilum PscAPtc. aestuarii PscAPld. luteolum PscAChl. tepidum PscAChl. phaeobacteroides 266 PscAChl. phaeo. PscAChl. limicola PscAChl. chlorochromatii PscAChl. vibrioforme PscAChl. phaeo BS1 PscAChl. limicola thio. PscA

10 20 30 40 50 60 70 80 90 100 110 120M A T K F P K F S Q D L A Q D P - T T R R I W Y A I A M A H D F E S H D G M T E E N L Y Q K I F A S H F G H L A I I F L W V S G S L F H V A W Q G N F E Q W V QM A T K F P K F S Q D L A Q D P - T T R R I W Y G I A T A H D F E T H D G M T E E N L Y Q K I F A S H F G H L A I I F L W T S G T L F H V A W Q G N F E Q W I K

M P S F L P C L R E R R D S M T I S P P E R E P K V R V V V D N D P V P T S F E K W A K P G H F D R T L A R G P Q T T T W I W N L H A L A H D F D T H T - S D L E D I S R K I F S A H F G H L A V V F I W L S G M Y F H G A K F S N Y E A W L AM A V K V D K D P V P T S F E K W G K P G H F D R T L A R G P K T T T W I W N L H A D A H D F D S Q T - S D L E D I S R K I F S A H F G H L A V V F V W L S G M Y F H G A K F S N Y E A W L N

M A T A D A A F N P - - - - - - - - - - - R A Q V F E W F K - D K V P A T R G A V L K A H I N H L G M V A G F V S F V L V H - - - - - - - - - - - -M A T A E M A F N P - - - - - - - - - - - R A Q V F E Y F K - D K V P A T R G A V L K A H I N H L G N I S A M V S F I L V H - - - - - - - - - - - -M A T A E M A F N P - - - - - - - - - - - R A Q V F E Y F K - D K V P A T R G A V L K A H I N H L G N V A A M V S F I L V H - - - - - - - - - - - -

M A S F S S - - - - - - - - - - Y A N G V K R W Y Q K L E L P M P P E R I F G A H M M L I G G L A C L I G T Y F F A S M T M W N - D G Y V NM A D Q A N P T G V M P K G K V P P P K G K V P P P K G N - G S P G S A S V V Q E K D A A K M K R F L F Q R T E T R S T K W Y Q I F D T D K LM A E Q V N P A G V K P K G T V P P P K G S A P S P K A S - G A S G G P S V I K E Q D A A K M R R F L F Q R T E T R S T K W Y Q V F D T D K IM A E Q V K P A G V K P K G T V P P P K G N A P A P K A N - G A P G G A S V I K E Q D A A K M R R F L F Q R T E T R S T K W Y Q I F D T E K LM A E Q V N P A G V K P K G S V P P P K G N A P S P K G N - G A S G G P S V I K E Q D A A K M R R F L F Q R T E T R S T K W Y Q I F D T E K LM A D Q A N P T G V K P K G Q V P P P K G K V P P P K G N G A P A G G A S I I K E K D A A K M R R F L F Q R T E T R S T K W Y Q V F D T D K VM A E Q V N P A G V K P K G S V P P P K G N A P S P K G N - G A P G G P S V I K E Q D A A K M R R F L F Q R T E T R T T K W Y Q V F D T D K LM A E Q V N P A G V K P K G T V P P P K G N A P S P K G N - G A A G A P S V I K E Q D A A K M R R F L F Q R T E T R S T K W Y Q I F D T D R VM A E Q V N P A G A V P K A K V P P P K G N A P S P K G D - G T S G A P S V I K E Q D A A K M R R F L F Q R T E T R T T K W Y Q I F D T D K IM A D Q A N P T G V K P K G Q V P P P K G K V P P P K G N G A P A G G A S I I K E K D A A K M R R F L F Q R T E T R S T K W Y Q V F D T D K VM A E Q V K P A G V K P K G T V P P P K G N A P A P K A N - G A P G G A S V I K E Q D A A K M R R F L F Q R T E T R S T K W Y Q I F D T E K I

M P S F L P C L R E R R D S M T I S P P E R E P K V V D D P V P T S F E K F M A . Q . N P A G V K P K G V P P P K G . P P K G . G S V . K E . D A A K M . R F L F Q R T E T R . T K W Y Q . F D T . K .


130 140 150 160 170 180 190 200 210 220 230 240D P V N T R P I A H A I W D P Q F G K A A V D A F T Q A G A S N P V D I - - - - - A Y S G V Y H W W Y T I G M R T N G D L Y Q G A I F L L I L A S L A L F A G W L H L Q P K F R P S L S W F K N A E S R L N H H L A G - - L F G V S S L A W A GD P L N V R P I A H A I W D P H F G Q G A I D A F T Q A G A S N P V N I - - - - - A Y S G V Y H W F Y T I G M T T N A D L Y Q G A V F L L I L S S L F L F A G W L H L Q P K F R P S L A W F K D A E S R L N H H L A G - - L F G V S S L A W T GD P T G I K P S A Q V V W P I V G - - - Q G I L N G D V G G G F H G I Q - - - - - I T S G L F Q L W R A S G I T N E F Q L Y C T A I G G L V M A G L M L F A G W F H Y H - K R A P K L E W F Q N V E S M L N H H L A G - - L L G L G S L A W A GN P T V I K P S A Q V V W P V V G - - - Q G I L N G D V G G G F S G I Q - - - - - I T S G L F Y L W R A A G F T N S Y Q L Y V T A I G G L V M A A L M V F A G W F H Y H - K A A P K L E W F Q N A E A M M N H H L S V - - L L G C G S L G W T G- - - - - - - - - H L S W L S - - - - - - D Q V L F A P T P - - - - - - - - - - - - - - I F Y A R L Y Q L G L D A S - A R S A D A L M V A R L H L L A A I I F W I I G H - I K T P R E D E F L K N V S F G K T L V A Q F H F L A L V A T L W G M- - - - - - - - - H L S W D P A T - - - - Q G V L W A P A T - - - - - - - - - - - - - - M F Y A R L Y Q L G M D A T - A L S P D A L F V A R M H L L A A I I L W G V G H - I K S P A E E K F L E K V T M G K S L V A Q F H F F A L I A T L W G L- - - - - - - - - H L S W D P A T - - - - Q G V L W A P A T - - - - - - - - - - - - - - M F Y A R L Y Q L G L D A T - A L S P D A L F V A R M H L L A A I I L W G F G H - V K S P A E E K F L E K V T M G K A L V A Q F H F F A L I A T L W G LL T L R P R L I A L G I Y D P Y D T E Q I Q R V W L P L I G E F S T S - - - - - - - K L P F F G Q Y P L T M T D F R L F G W G C F H I G L G L W L V Y A G A A H Y Y G A R G G A T I G E I F W L L P Y V P G L K G L C Q I K W F T P D G P W Y KD D E Q V V G - A H L A L L G V L G F V M G I Y Y L S G M Q V M P W G - - - A P G F H D N W F Y L T I K P R M V S L G I D T Y S P K T E D L M A A S T K L M G W A I L H - F V S G S I L L F G - G W R H W T H N L T N P F T G R A G N F R D F RD D E Q V V G - G H L A L L G V L G F L M A I Y Y F S G I Q V F P A W L G G M P G F H D N W F Y L T I K P R M V S L G I D T Y S T Q T A D L Q Q A A T N L I G W A A F H - F L S G S I L L F G - G W R H W T H N L T N P F T G R A G N F R D F RD D E Q V V G - G H L A L L G V L G F I M G I Y Y I S G I Q V F P W G A P G - - - F H D N W F Y L T I K P R M V S L G I D T Y S T K T A D L E A A G A R L L G W A A F H - F L V G S V L I F G - G W R H W T H N L T N P F T G R C G N F R D F RD D E Q V V G - G H L A L L G V M G F L M A I Y Y I S G I Q V F P W G A P G - - - F H D N W F Y L T I K P R M V S M G I D T Y S T R S A D L E Q A A T N L L G W A L L H - V I S G S I L L F G - G W R H W T H N L T N P F T G R A G N F R D F RD D E Q V V G - G H L A L L G V L G F I M G I Y Y L S G M Q V L P W N G - - A P A F H D N W F Y L T I K P R M V S L G I D T Y S P R T E D L I A A S W K L M G W A I L H - F V S G S I L L F G - G W R H W T H N L T N P F T G R A G N F R E F RD D E Q V V G - G H L A L L G V M G F L M A I Y Y I S G I Q V F P G G Y A G - - - F H D N W F Y L T I K P R M V S L G I D T Y S T K T A D L E Q A A I N L L G W A L L H - V I S G S I L L F G - G W R H W T H N L T N P F T G R A G N F R D F RD D E Q V V G - A H L A L L G F L G Y L M A I Y Y I S G V Q V F P W G A P G - - - F Q D N W F Y L T I K P R M V S L G I D T Y S T K T E D L Q W A A S N L L G W A L F H - I I S G S I L I F G - G W R H W T H N L T N P F T G R A G N F R D F RD D E Q V V G - G H L A L L G V L G F L M A I Y Y I S G I Q V F P A F L G G G P G F H D N W F Y L T I K P R M V S L G I D T Y S T N T A D L Q Q A A T N L L G W A A F H - F L S G S I L L F G - G W R H W T H N L T N P F T G R A G N F R D F RD D E Q V V G - G H L A L L G V L G F I M G I Y Y L S G M Q V L P W N G - - A P A F H D N W F Y L T I K P R M V S L G I D T Y S P R T E D L I A A S W K L M G W A I L H - F V S G S I L L F G - G W R H W T H N L T N P F T G R A G N F R E F RD D E Q V V G - G H L A L L G V L G F I M A I Y Y I S G I Q V F P W G A P G - - - F H D N W F Y L T I K P R M V S L G I D T Y S T K T A D L E W A G T K L L L W A G Y H - F I A G S V L I F G - G W R H W T H N L T N P F T G R C G N F R D F RD D E Q V V G I G H L A L L G V . G F . M . I Y Y . S G . Q V F P . P G A P G F H D N W F Y L T I K P R M V S G I D T Y S . . D L A . L . G W A . . H P . S G S . L . F G G W R H W T H N L T N P F T G R . G N F R . F R


250 260 270 280 290 300 310 320 330 340 350 360H L I H V A I P E S R G - Q H V G W D N F L S T M P H P A G L A P F F T G N W G V Y A Q - - N P D T A S H V F G T A Q G A G T A I L T F L G G F H P Q T E S L W L T - D M A H H H L A I A V L F I V A G H M Y R T Q F G I G H S I K E M M D A KH L V H V A I P E S R G - V H V G W D N F L S V K P H P A G L M P F F T G N W G V Y A Q - - N P D T A G H V F G T S Q G A G T A I L T F L G G F H P Q T E S L W L T - D I A H H H L A I A V L F I I A G H M Y R T N V R I G H S I K E I L N A HH Q I H V S L P I N K L - L D A G V A A K D I P L P H E F I L N P S L M A E L Y P K V D W G F F S G V I P F F T F N W A A Y S D F L T F N G G L N P V T G G L W L S - D T A H H H L A I A V L F I I A G H M Y R T N W G I G H S L K E I L E A HH L I H V S L P V N K L - L D S G V A A K D I P L P H E F F D - A S V M A E L Y P - - - - S F A Q G L K P F F T L D W A A Y S D F L T F K G G L N P T T G S L W L S - D V A H H H L A I A V L F I I A G H M Y R T G F G I G H S M K E I L E A HH M A Y I G V R G A N G G I V P T G L S F D M F G P I T G A T L A G N H V A F G A L L - - - F L G G V F H H F A - - - G F N T K R F A F F E K D W E A V L S V S A Q - V L A F H F A T V V F A M I I W N R P D Q P I L S F Y F M Q D Y A L S N YH M A F Y G I L G P E G K L A P T G L S F D M F G P I T P A S M A G N H V A F G A V F - - - F L G G I F H Y F A - - - G F N T K K F A F F E K D W E A V L S V S C Q - V L A F H F A T V V F A M I I W Q H P - - - Q L G F G F M R E Y A V S Q YH M A F Y G I L G P S G K L E P T G L S F D M F G P I T P A T M A G N H V A F G A V F - - - F L G G I F H Y F A - - - G F N T K R F A F F E K D W E A V L S V S C Q - I L A F H F A T V V F A M I I W Q H P - - - Q L G F G F M R E Y A V S Q YV G L P W G S F A N T P W R I L R R T Y A D A L G A H T I Y I G L L F - F I W G F V L W F V L D K P P V P L Q P - - - A Q V M T P N G P M P I E Q A P F P Y G W F D P Y L N E V M H P M N T I N G E T T M S F V W G V L F V V L G A Y W W Y R PF L G K F G D A V F Q G - - T S A K T Y K D A L G P H T V Y M A L L F - L G W G F M L W G V L G I A P I P D F Q T S N S E T F M S F V W F V V F F A L G L Y W W K N P P N A A T H L N D D M K A A F S V H L T A I G Y V N I A L G I I A F V A FF L G K F G D M V F N G - - T S A K T Y K D A L G P H A V Y M S L L F - L G W G L L M W F V L G F A P V P D F Q T I N S E T F M S F V W F V A F F A L G I Y W W N N P P N A A I H L N D D M K A A F S V H L T A I G Y I N I A L G V I A F V A FF L G K F G D V V F N G - - T S A K S Y K E A L G P H A V Y M S L L F - L G W G I V M W A I L G F A P I P D F Q T I N S E T F M S F V F A V I F F A L G I Y W W N N P P N A A I H L N D D M K A A F S V H L T A I G Y I N I A L G C I A F V A FF L G K F G D V V F S G - - T S A K S Y K D A L G P H A V Y M S L L F - L G W G L I M W L A L G W A P V P D F Q T I N S E T F M S F V W F V V F F A L G I Y W W N N P P N A A T H L N D D M K A A F S V H L T A I G Y I N I A L G V I A F V A FF L G K F G D A V F Q G - - T S A K T Y K D A L G P H A V Y M S L L F - L G W G L M L W I V L G F A P I P D F Q T I N S E V F M S F V W F L V F F A L G L Y W W K N P P N A A I H L N D D M K A A F S V H L T A I G Y V N I A L G I I A F V A FF L G K F G D M A F N G - - T S A K T Y K D A L G P H T V Y M S L L F - L G W G L V M W F A L G I P P V P D F Q T I N S E P F M S F V F A V L F F A L G I Y W W N N P P N A A I H L N D D M K A A F S V H L T A I G Y I N I A L G V I A F V A FF L G K F G D V V F S G - - T S A K S Y K D A L G P H T V Y M S L L F - L G W G F V M W F V L G F A P I P D F Q T I N S E T F M S F I F A V I F F A A G I Y W W N N P P N A A T H L N D D M K A A F S V H L T A I G Y I N I A L G C I A F V A FF L G K F G D A V F N G - - T S A K T Y K D A L G P H A V Y M S L L F - L G W G L L M W L V L G Y A P V P D F Q T I N S E T F M S F V W F A V F F A L G L Y W W N N P P N A A I H L N D D M K A A F S V H L T A I G Y I N I A I G V I A F V A FF L G K F G D A V F Q G - - T S A K T Y K D A L G P H A V Y M S L L F - L G W G L M L W I V L G F A P I P D F Q T I N S E V F M S F V W F L V F F A L G L Y W W K N P P N A A I H L N D D M K A A F S V H L T A I G Y V N I A L G I I A F V A FF L G K F G D M V F S G - - T S A K S Y K E A L G P H A V Y M S L L F - L G W G L V M W L I L G F A P I P D F Q T I N S E T F M S F V F A V I F F A L G I Y W W N N P P N A A I H L N D D M K A A F S V H L T H R - L H Q H R A W V I A F V A FF L G K F G D V F G T S A K . Y K D A L G P H V Y M S L L F L G W G . . W V L G . P . P D F Q T I N S E F M S F . F . . F F A . G . Y W W N P P N A A H L N D D M K A A F S V H L T A I G . . . . . . G I A F V A F


370 380 390 400 410 420 430 440 450 460 470 480D F F G T K V E G P F N M P H Q G I Y E T Y N N S L H F Q L G W H L A C L G V I T - - - S L V A Q H M Y S - L P P - - Y A F I A Q D H T T M A A L Y T H H Q Y I A G F L M V G A F A H G A I F L V R D Y D P A Q N K G N V L D R V L Q H K E A IQ P P - - - - S G K L G A G H K G L Y D T V N N S L H F Q L G L A L A S L G V I T - - - S L V A Q H M Y S - L P S - - Y A F I A K D Y T T Q A A L Y T H H Q Y I A G F L M V G A F A H G A I F F V R D Y D P E A N K D N V L Y R M L E H K E A LK G P - - - - - - F T G A G H K G L Y E V L T T S W H A Q L A I N L A M M G S L S - - - I I V A Q H M Y A - M P P - - Y P Y L A T D Y P T Q L S L F T H H M W I G G F L V V G G A A H G A I F M V R D Y D P A M N Q N N V L D R V L R H R D A IK G P - - - - - - F T G E G H K G L Y E I L T T S W H A Q L A V N L A L L G S L T - - - I V I A H H M Y S - M P P - - Y P Y M A T D Y G T Q L S L F T H H T W I G G F L I V G A G A H G A I F M V R D Y D P A K N V N N L L D R V I R H R D A IA A P - - - E I R E I A S Q N P G F L I K Q V I L G H L V F G V M F W I G G V F H G - - A H F M L R A T N - D P K - - L A E A L K D F K M L K R C Y D H D F Q K K F L A L I M F G A F L P I F V S Y G - - - I A T H N T I A D L H H L A K A G MA G P - - - E L K M I A Q G N P G L L V K Q A I L G H L V M G I M F W I G G V F H G - - A H F M L R V L N - D P K - - M A E E M K D F K F I K R C Y D H E F Q K K F L A L I M F G A F L P I F V A Y G - - - I A T H N T I A D I H A A A K T G IA G P - - - E L K M I A Q S N P G L L V K Q A I L G H L V M G I M F W I G G V F H G - - A H F M L R V L N - D P K - - L A E E M K D F K F I K R C Y D H E F Q K K F L A L I M F G A F L P I F V S Y G - - - I A T H N T I A D I H A A S K T G LP R S - - I N I T H L E D T K A V F H V H L T S I G Y V S F A L A I V G F L A L R N H P S Y L I L N D M N V I I Y G K K I V N P G R M I H N M I T F N H V Q V G L L Y V A A G V F H G G Q Y L H G L N I S G A Y K Q A R S K F I T W F Q N P D LQ Q D - S F A P Y Y A E L D K L V F Y I Y G E P F N R V S F D F V Q E G G K V I A G S K E F E E F A A Y A I L P K N G E S F G M A R V V I N L I T F N H I I C G V L Y V F A G V Y H G G Q Y L L K I Q I N G L Y S Q I K S I W V A K G R D Q D LQ Q P - S F A P Y Y K E L D K L V F Y L Y G E P F N R V S Y D F V Q E G G R I V S G S K E F S E F A P F A I L P K N G A S F G M S R V V I N L I T F N H I I C G V L Y V F A G V Y H G G Q Y L L K I Q L N G L Y N Q I K S I W I T K G R N Q E VQ Q P - S F A P Y Y K E L D K L V F Y L Y G E P F N R V S F N F V E Q G G K V I S G A K E F A D F P A Y A I L P K S G E A F G M A R V V T N L I V F N H I I C G V L Y V F A G V Y H G G Q Y L L K I Q L N G M Y N Q I K S I W I T K G R D Q E VQ Q P - S F A P Y Y K E L D N L V F Y L Y G E P F N R V S Y D F V Q E G G Q I V S G S K E F T E F A P Y A I L P K N G E A F G M S R V V I N L I T F N H I I C G V L Y V F A G V Y H G G Q Y L L K I Q L N G L Y N Q I K S I W I T K G R D Q E VQ Q D - S F A P Y Y A E L D K L V F Y I Y G E P F N R V S F D F V E Q G G K V L S G S K E F A E F P A F A I L P K N G E S F G M A R V V I N L I V F N H I I C G V L Y V F A G V Y H G G Q Y L L K V Q I N G L Y S Q I K S I W V A K G R D Q E LQ Q P - S F H A Y Y K E L D N L V F Y L Y G E P F N R V S Y D F V Q E G G R I I S G K K E F V E F A P Y A I L P K N G Q A F G M S R V V I N L I T F N H I I C G V L Y V F A G V Y H G G Q Y L L K I Q L N G L Y N Q I K S I W I T K G R D Q E VQ Q P - S F A D Y Y K A L D S L V F Y I Y G E P F N R V S Y D Y V E A G G R I V S G S K E F A D F P A Y A I L P K D G A A F G M S R T V I N L I V F N H I I C G V L Y V F A G V Y H G G Q Y L L K I Q L N G L Y S Q I K S V F I T K G R D Q E VQ Q P - S F A P Y Y Q E L D N L V F Y L Y G E P F N R V S Y D F V Q E G G R I V S G S K E F A E F A P F A I L P K N G Q S F G M S R V V I N L I S F N H I I C G V L Y V F A G V Y H G G Q Y L L K I Q L N G L Y N Q I K S I W I T K G R D Q E VQ Q D - S F A P Y Y A E L D K L V F Y I Y G E P F N R V S F D F V E Q G G K V L S G S K E F A E F P A F A I L P K N G E S F G M A R V V I N L I V F N H I I C G V L Y V F A G V Y H G G Q Y L L K V Q I N G L Y S Q I K S I W V A K G R D Q E LQ Q P - S F A P Y Y K E L D K L V F Y I Y G E P F N R V S F N F V E Q G G K V I S G A K E F A E F P A Y A I L P R N G E S F G M A R V V T N L I T F N H I I C G V L Y V F A G V Y H G G Q Y L L K I Q L N G I Y N Q I K S I W I T K G R D Q E VQ Q P G S F A Y Y E L D . L V F Y . Y G E P F N R V S . . V G G V . S G S K E F . . F Y A I L P K N G . F G M R V N L I F N H I I C G V L Y V F A G V Y H G G Q Y L L K . Q . N G . Y N Q I K S . . I K G R Q . .


490 500 510 520 530 540 550 560 570 580 590 600I S H L S W V S L F L G F H T L G L Y V H N D V V V A F G T P E K Q I L I E P V F A Q F I Q A A H G K L L Y G F D T L L S N P D S I A S T A W P N Y G N V W L P G W L D A I N S G T N S L F L TI S H L S W V S L F L G F H T L G L Y V H N D V V V A F G T P E K Q I L I E P V F A Q F V Q A A S G K A L Y G F D V L L S N A D S I A S N A S A A Y - - - - L P G W L D A I N S G S N S L F L NI S H L N W V C I F L G F H S F G L Y V H N D T M R A F G R P Q D M F S D T G I Q L Q P V F A Q W V Q N L H T L A P G G T A P N A A A T A S V A F G G D V V A V G G K V A M M P I VI S H L N W V C I F L G F H S F G L Y I H N D T M R A L G R P Q D M F S D S A I Q L Q P I F A Q W I Q N I H T L A P G G T A P N A I A S A S Q V F G G D V V A I G N K V A L M P I TF A N M T Y I N I G T P L H D A I F G S H GF A H M T Y I N I G T P L H D A V F G S K GF A H M T Y I N I G T P L H D A I F G S K GQ T K I V G T T M F V S F V T V V F - G Y G M I C W N T G A E L D L N F G I Y Q F R S F R A I Q M D G E A G N I G Y R V F R P N N P W D P T A G G D W V K N P D G T A K L V K A R N L Q V G D R I L N E E L G I G S S P T Y S F T TQ V K V L G T I M A L C F A T M L S - V Y A V I V W N T I C E L N I F G T N - - - - I M M S F Y W L K P L P F L H W M F E D PQ V K I L G T V M A L C F A T M L S - V Y A V I V W N T I C E L N I F G T N - - - - I M M S F Y W L K P L P M F Q W M F A D PQ V K I L G T V M A L C F A T M L S - V Y A V I V W N T I C E L N I F G T N - - - - I T M S F Y W L K P L P I F Q W M F A D PQ V K I L G T V M A L C F A T M L S - V Y A V I V W N T I C E L N I F G T N - - - - I M M S F Y W L K P L P M F Q W M F A D PQ V K I L G T I M A L C F A T M L S - V Y A V I V W N T I C E L N F F G T N - - - - I M M S F Y W L K P L P F L H W M F E D PQ V K I L G T V M A L C F A T M L S - V Y A V I V W N T I C E L N I F G T N - - - - I M M S F Y W L K P L P I F Q W M F T D PQ V K I L G T V M A L C F A T M L S - V Y A V I V W N T I C E L N L F G T N - - - - I M M S F Y W L K P L P M F Q W M F N D PQ V K I L G T V M A L C F A T M L S - V Y A V I V W N T I C E L N I F G T N - - - - I M M S F Y W L K P L P M F Q W M F A D PQ V K I L G T I M A L C F A T M L S - V Y A V I V W N T I C E L N F F G T N - - - - I M M S F Y W L K P L P F L H W M F E D PQ V K I L G T V M A L C F S T M L S - V Y A V I V W N T I C E L N L F G T N - - - - I T M S F Y W L K P L P I F Q W M F A D PQ V K I L G T . M A L C F . T M L S Y V Y A V I V W N T I C E L N F G T N F I M M S F Y W L K P L P F W M F D P N I A S G V G A N S L F L I L N E E L G I G S S P T Y S F T T

I

II

III IV

V VI

VII

Figure 2S

Figure 3S

PscA/PshA/PsaA/PsaB C-terminal Alignments

T. elongatus PsaBSynechococcus 7002 PsaBT. elongatus PsaASynechococcus 7002 PsaAHbc. mobilis PshAHbt. gestii PshAHbt. modesticaldum PshACab. thermophilum PscAPtc. aestuarii PscAPld. luteolum PscAChl. tepidum PscAChl. phaeobacteroides 266 PscAPld. phaeo. PscAChl. limicola PscAChl. chlorochromatii PscAChl. vibrioforme PscAChl. phaeobacteroides BS1 PscAChl. limicola thio. PscA

10 20 30 40 50 60 70 80 90 100I G P G D F L V H H A I A L G L H T T T L I L V K G A L D A R G S K L M P D - - - K K D F G Y A F P C D G P G R G G T C D I S A W D A F Y L A M F W M L N T I G W V T F Y W H W K H L G V W E G N V A QI G P G D F L V H H A I A L G L H T T T L I L V K G A L D A R G S K L M P D - - - K K D F G Y S F P C D G P G R G G T C D I S A W D A F Y L A M F W M L N T L G W L T F Y W H W K H L G I W Q G N V A QL G T A D F M V H H I H A F T I H V T V L I L L K G V L F A R S S R L I P D - - - K A N L G F R F P C D G P G R G G T C Q V S G W D H V F L G L F W M Y N C I S V V I F H F S W K M Q S D V W G T V A PL G T A D F M V H H I H A F T I H V T V L I L L K G L L Y S R S S R L V P D - - - K G Q L G F R F P C D G P G R G G T C Q V S G W D H V F L G L F W M Y N S L S I V I F H F S W K M Q S D V W G T I L PT V S D F V A A H A I A G G L H F T M V P L W R M V F F S K V S P W T T K V G M K A K R D G E F P C L G P A Y G G T C S I S L V D Q F Y L A I F F S L Q V I A P A W F Y L D G C W M G S F V A T S S ES I S E F V A A H A I A G G L H F T M V P M W R M V F F S K V S P W T T K V G M K A K R D G E F P C L G P A Y G G T C S I S L V D Q F Y L A I F F S L Q V I A P A W F Y I D G C W M G S F V A V A Q PS I S E F V A A H A I A G G L H F T M V P M W R M V F F S K V S P W T T K V G M K A K R D G E F P C L G P A Y G G T C S I S L V D Q F Y L A I F F S L Q V I A P A W F Y I D G C W M G S F V A V A A PE I N D A I F F W L M I G G W L F G F I P L L R I A F H N Y Q S P W Y R D F E W R K Q S P - D F P C I G P V K G G T C G V S I Q D Q M W F C I L F S I K P L S A I A W Y L D G G W I A T M M A R G - -S I N D W V M A H V I V A G S L F S L I A L A R I A F F S H T S P L W D D L G L K K N S Y - S F P C L G P V Y G G T C G V S I Q D Q L W F A M L W G V K G L S A V C W Y I D G A W I A S M M Y G V P -S I N D W V M V H V I V A G S L F S L I A L V R I A F F A H T S P L W D D L G L K K N S Y - S F P C L G P V Y G G T C G V S I Q D Q L W F A M L W G V K G L S A V C W Y I D G A W I A S M M Y G V P -S I N D W V M A H V I T A G S L F S L I A L V R I A F F A H T S P L W D D L G L K K N S Y - S F P C L G P V Y G G T C G V S I Q D Q L W F A M L W G I K G L S A V C W Y I D G A W I A S M M Y G V P -S I N D W V M A H V I T A G S L F S L I A L V R I A F F A H T S P L W D D L G L K K N S Y - S F P C L G P V Y G G T C G V S I Q D Q L W F A M L W G V K G L S A V C W Y I D G A W I A S M M Y G V P -S I N D W V M V H V I V A G S L F S L I A L V R I A F F A H T S P L W D D L G L K K N S Y - S F P C L G P V Y G G T C G V S I Q D Q L W F A M L W G I K G L S A V C W Y I D G A W I A S M M Y G V P -S I N D W V M A H V I T A G S L F S L I A L V R I A F F S H T S P L W D D L G L K K N S Y - S F P C L G P V Y G G T C G V S I Q D Q L W F A M L W G V K G L S A V C W Y I D G A W I A S M M Y G V P -S I N D W V M V H V I T A G S L F S L I A L V R I A F F S H T S P L W D D L G L K K N S Y - S F P C L G P V Y G G T C G V S I Q D Q L W F A M L W G I K G L S A V C W Y I D G A W I A S M M Y G V P -S I N D W V M V H V I V A G S L F S L I A L V R I A F F A H T S P L W D D L G L K K N S Y - S F P C L G P V Y G G T C G V S I Q D Q L W F A M L W G V K G L S A V C W Y I D G A W I A S M M Y G V P -S I N D W V M A H V I V A G S L F S L I A L A R I A F F S H T S P L W D D L G L K K N S Y - S F P C L G P V Y G G T C G V S I Q D Q L W F A M L W G V K G L S A V C W Y I D G A W I A S M M Y G V P -S I N D W V M A H V I T A G S L F S L I A L V R I A F F A H T S P L W D D L G L K K N S Y - S F P C L G P V Y G G T C G V S I Q D Q L W F A M L W G I K G L S A V C W Y I D G A W I A S M M Y G V P -S I N D W V M . H V I . A G S L F S L I A L V R I A F F H T S P L W D D L G L K K N S Y S F P C L G P V Y G G T C G V S I Q D Q L W F A M L W G . K G L S A V C W Y I D G A W I A S M M Y G V P P


110 120 130 140 150 160 170 180 190 200F N - - - - - - - - - - - - - - E S S T Y L M G W L R D Y L W L N S S Q L I N G Y N P F G T N N L S V W A W M F L F G H L V W A T G F M F L I S W R G Y W Q E L I E T - - - - L V W A H E R T P L A N LF N - - - - - - - - - - - - - - E K S T Y L M G W F R D Y L W A N S A Q L I N G Y N P Y G V N N L S V W A W M F L F G H L V W A T G F M F L I S W R G Y W Q E L I E T - - - - I V W A H E R T P L A N ID G T V - - - S H I T G G N F A Q S A I T I N G W L R D F L W A Q A S Q V I G S Y G - - - - S A L S A Y G L L F L G A H F I W A F S L M F L F S G R G Y W Q E L I E S - - - - I V W A H N K L K V A P AD G S V - - - S H V T G G N F A T S A I T I N G W L R D F L W A Q S S Q V I N S Y G - - - - S A L S A Y G I M F L A G H F V F A F S L M F L F S G R G Y W Q E L I E S - - - - I V W A H N K L K L A P AV Y K Q A A E L F K A N P T W F S L H A V S N F T S E G Y F S Y I I S Q T T R M F K Y Y - - - - D G H L V Q A L L G A H F I W A F T F S M L F Q Y R G S R D E G A - - - - M V L K W A H E Q V G L G F AN N E I Y Q A A L A A Y N A H N P L H Q L S P L T N M G Y F S Y I I Q Q T T A M F S R Y - - - - D G H M I Q A L L G A H F I W A F T F S M L F Q Y R G S R D E G S - - - - M V L K W A H E Q V G V G F AY N D I Y Q A A L A T F N S H N P L H Q L S P L T N M G Y F S Y I I Q Q T T A M F S R Y - - - - D G H M I Q A L L G A H F I W A F T F S M L F Q Y R G S R D E G A - - - - M V L K W A H E Q V G V G F A- - - - - - - - - - - - - N E A Y Y L T H N I S H T G G V F L Y M W N E T T W I W T - - - - - - D N H L T A M L L L G H L I W F V S F A L W F K D R G S R A E G G D I Q S R W V R L M G K R L G I K T L- - - - - - - - A A D A K S W D A V A G L S H H Y S A G I F Y Y F W T E T V T I F S - - - - - - S S H L S V I L M I G H L I W F I S F A V W F E D R G S R L E G A D I Q T R T V R W L G K K F L N R D V- - - - - - - - A A D A K A W D S V A G L A H H Y T S G I F Y Y F W T E T V T I F S - - - - - - S S H L S T I L M I G H L V W F I S F A V W F E D R G S R L E G A D I Q T R T I R W L G K K F L G R D V- - - - - - - - A A D A K A W D S I A H L H H H Y T S G I F Y Y F W T E T V T I F S - - - - - - S S H L S T I L M I G H L V W F I S F A V W F E D R G S R L E G A D I Q T R T I R W L G K K F L N R D V- - - - - - - - V A D A K A W D S V A G L Q H H Y T S G I F Y Y F W T E T V T I F S - - - - - - S S H L S T I L M I G H L I W F I S F A V W F E D R G S R L E G A D I Q T R T V R W L G K K F L N R D V- - - - - - - - A A D A K A W D S V A G L Q H H Y T S G I F Y Y F W T E T V T I F S - - - - - - S S H L S T I L M I G H L V W F I S F A V W F E D R G S R L E G A D I Q T R T I R W L G K K F L N R D V- - - - - - - - V A D A K A W D S V A G L A H H Y T S G I F Y Y F W T E T V T I F S - - - - - - S S H L S V I L M I G H L I W F I S F A V W F E D R G S R L E G A D I Q T R T I R W F G K K F L N R D V- - - - - - - - A A D A K A W D S V A G L Q H H Y T S G I F Y Y F W T E T V T I F S - - - - - - S P H L S T I L M I G H L V W F I S F A V W F E D R G S R L E G A D I Q T R T I R W L G K K F L N R D V- - - - - - - - A A D A K A W D S V A G L A H H Y T S G I F Y Y F W T E T V T I F S - - - - - - S S H L S T I L M I G H L V W F I S F A V W F E D R G S R L E G A D I Q T R T I R W L G K K F L G R D V- - - - - - - - A A D A K A W D A V A G L S H H Y S A G I F Y Y F W T E T V T I F S - - - - - - S S H L S V I L M I G H L V W F I S F A V W F E D R G S R L E G A D V Q T R T I R W L G K K F L N R D V- - - - - - - - A A D A K A W D S I A H L Q H H Y T S G I F Y Y F W T E T V T I F S - - - - - - S S H L S T I L M I G H L V W F I S F A V W F E D R G S R L E G A D I Q T R T I R W L G K K F L N R D VN Y Q A A A D A K A W D . . A . L H H Y . G I F Y Y F W T E T V T I F S Y G S S H L S I L M I G H L V W F I S F A V W F E D R G S R L E G A D I Q T R T I R W . G K K F L . R D V


210 220 230 240 250 260 270 280 290 300V R W K D K P V A L S I V Q A R L V G L A H F S V G Y I L T Y A A F L I A S T A A K F GV R W K D K P V A L S I V Q A R L V G L A H F T V G Y V L T Y A A F L I A S T S G K F GI Q P R - - - - A L S I I Q G R A V G V A H Y L L G G I A T T W A F F L A R I I S V GI Q P R - - - - A L S I V Q G R A V G V A H Y L L G G I V T T W A F F L A R S L S I GG K V Y - - N R A L S L K E G K A I G T F L F F K M T V L C M W C L A M VG K V Y - - N R A L S L K E G K A I G T F L F F K M T I V C M W C L A M VG K M Y - - N R A L S L K E G K A I G C F L F F K M T I V C M W A L A M VQ E V R - - F P V S N L A T A K L W G T V F F Y T G T F V L V F L Y F A D G F F Q N RT F R - - - F P V L T I S D S K L A G T F L Y F G G T F M L V F L F I A N G F Y Q T N T P A L P P V G D A A I S G Q Q L L T Q V V D F I V K L I AN F R - - - F P V L T I S D S K L A G T F L Y F G G T F M L V F L F M A N G F Y Q T D S P L P P T V S D A A V S G Q V L L T Q V V D Y L M K L I AN F R - - - F P V L T I S D S K L A G T F L Y F G G T F M L V F L F L A N G F Y Q T N S P L P P P V S H A A V S G Q Q M L A Q L V D T L M K M I AT F R - - - F P V L T I S D S K L A G T F L Y F G G T F M L V F L F I A N G F Y Q T N S P L P P T V G D A S L S G Q Q M L T Q V V D Y L M K L I AT F R - - - F P V L T I S D S K M A G T F L Y F S G V F M L V F L F I G N G F Y Q T D S P L P P Q V G D A S V S G Q V M L T Q V V D Y L M K L I AT F R - - - F P V L T I S D S K L A G T F L Y F G G T F M L V F L F I G N G F Y Q T D S P L P P P V A E A A V S G Q Q M L T Q V V D F L I K L V AT F R - - - F P V L T I S D S K M A G T F L Y F G G V F M L V F L F I G N G F Y Q T D S P L P P Q V G D A S V S G Q M M L T Q V V D Y L L K L I AQ F R - - - F P V L T I S D S K L A G T F L Y F G G T F M L V F L F L A N G F Y Q T D S P L P P T V S D A A V S G Q A L L T Q V V D Y L M K L I AQ F R - - - F P V L T I S D S K L A G T F L Y F G G T F M L V F L F I A N G F Y Q T N T P A L P P V G D A A V S G Q Q L L T Q V V D F I M K L I AN F R - - - F P V L T I S D S K L A G T F L Y F G G T F M L V F L F I A N G F Y Q T N S P L P P P V S H A A V S G Q Q M L A Q L V D T L M K M I AF R D K F P V L T I S D S K L A G T F L Y F . G T F M L V F L F . . N G F Y Q T S P L P P V D A A V S G Q Q M L T Q V V D L M K L I A

VIII IX

X

XI

FX

P840P780P700

PscB Alignments

Cab. thermophilum PscBChl. tepidum PscBChl. limicola 245 PscBChl. phaeobacteroides PscBPtc. aestuarii PscB

10 20 30 40 50 60M A A E E K A E M A K D A S A E A P A E K A A T K A A A K T A A K S - - - - - - - - - -

M A E P V E N K N Q A P A P G A K V P P K G A P A A P K A G A P A A P K G P V A P K A G A P A A K T G A S A A K Q A G KM A E P V K Q P D I S P K P A S K A A P A A P K A S V A P K A A T P A A K Q D Q A K P K A S S P K P - - - - - - S S G KM A E P V K Q P D V P P A P V S K A A P A A P K A A P K A A S P A A K Q G E - V K P K I S A A P K Q - - - - - - T S G KM A D P V K K Q E - - - - - T A S G P A K A K P A A P K A K A D A P K G K P A P K A G G A S S L V K G - - - - - - - - -M A E P V K . . P A P . K A A P A A A P K A A A P K . K . . A . A . K G A S A A K S G K


70 80 90 100 110 120- - - - - - - - - - - - - - - - - A A A K A P A K A A A K A A P P P I P G - - - - - - - - - - - - P P S R - - - - - - -P R L A S L G V T L G R S G V R Q E S A L P Y V K P K A V P P P K P A A P - A A K G A P A P K G A P A A P - - - - - - -L R L E A L N V N L G R T G I R Q E S A L P V K K A A P K A A P K P A P G - A K P A A P A A K A A P A V G - - - - - - -I R L E A L N V N L G R T G I R Q E S A L P V K K A P P K A A P K P A P G - G A K P A P G A K P A P K T A G A P A G T P- R L D S L D V N L G R T G R R M E S A L P V V R P K P K A A P K L A P K G A P K G A P A G K A V P K G A P A A K G - -R L . L V N L G R T G . R Q E S A L P V . K A P K A A P K P A P G G A K . A P A . K . A P . . A G T P


130 140 150 160 170 180- - - - - L S R P K T A A P K K K R E R Q I Y T I I E E L C I G C G F C T D E C P P K V N A I L P R D V E A V L D G G E- - A A K A A P G A P V A K A A P K A K K H Y F I I E N L C V G C G L C L D K C P P K V N A I G Y K F Y G D V Q E G G F- - A A P A P K G A P V A K A A P R A K K H Y F I I E N L C V G C G L C L D K C P P K V N A I G Y K F Y G D V Q E G G FV A K S A P A A G T P L A K A A P R A K S H Y F I I E N L C V G C G L C L D K C P P K V N A I G Y K F Y G D V Q E G G F- A P A A K P A A K P I A K K A P R P K K H Y F I L E N L C V G C G L C L D T C P P K V N A I G Y K F Y G D V Q E G G FV A A . G P . A K A A P R A K K H Y F I I E N L C V G C G L C L D K C P P K V N A I G Y K F Y G D V Q E G G F


190 200 210 220 230 240T Y W I D Q T R C I S C S L C F V A G T C P T D A V V F T E G G V S R T Q Y M E D Y M H I E L V D E P Y W R T R D E L SR C Y I D Q A A C I S C S A C F S G D E C P S G A L I E V L P D G E V L D F S Y T P P E R L D F D L R F L H R F H R E AR C Y V D Q D A C I S C S A C F S G D E C P S G A L I E V L P D G Q I L D F S Y T P P E R L D F D L R F L H R F H R E AR C Y I D Q D A C I S C S A C F S T D E C P S G A L I E V L P D G Q V L D F S F T P P E R L D F D L R F L H R F H R E VR C Y I D Q D A C I S C A A C F S S D E C P S G A L I E I L P T G E V L D F T Y T P P E R L D F D L R F L H R F H K E SR C Y I D Q D A C I S C S A C F S . D E C P S G A L I E V L P D G V L D F S Y T P P E R L D F D L R F L H R F H R E .


250 260 270 280 290 300R L G S I L *RRRRR L G S I L

Figure 4S

Figure 5S FmoA Alignments

Cab. thermophilum FmoAChl. tepidum FmoAChl. limicola FmoAChl. phaeobacteroides FmoAPtc. aestuarii FmoA

10 20 30 40 50 60M G S K L T T A Y Y D L D L D A T G - - - - W G T L R A E A R I S N V P S A S P L L P I D G E L K L E A K - - K

M A L F G S N D V T T A H S D Y E I V L E G G S S S W G K V K A R A K V N - A P P A S P L L P A D C D V K L N V K P L DM A L F G T K D T T T A H S D Y E I I L E G G A S S W G K V K G R A K V N - V P P A S P L L P A D C N V K I N V K P L DM A L F G T K D T T T A H S D Y E I I L E G G S S S W G K V K G R A K V N - V P P A S P L L P A D C N V K I N V K P L DM A L F G T K D T T T A H S D Y E I I L E G G S S S W G Q V K G R A K V N - V P A A L P L L P A D C N I K I E A K P L DM A L F G T K D T T T A H S D Y E I I L E G G S S S W G K V K G R A K V N N V P P A S P L L P A D C N V K I N V K P L D


70 80 90 100 110 120I E D D V V R L T Y F G Q S I V D G I L G R V E G E T D I A N E S P T R R V A A G E G K I T V G K F S H R F E F E G V VP A K G F V R I S A V F E S I V D S T K N K L T I E A D I A N E T K E R R I S V G E G M V S V G D F S H T F S F E G S VP A K G F V R F S A V I E S I V D S T K N K L V V E A D I A N E T K E R R I C V G E G S V S V G D F S H S F S F E G S VP A K G F V R F S A V I E S I V D S T K N K L V V E A D I A N E T K E R R I C V G E G S V T V G D F S H S F S F E G S VT Q K G V V R F T S T I E S I V D S T K N K L V V E V D I A N E T K D R R V A V G E G E V T V G D F S H K F S F E G S VP A K G F V R F S A V I E S I V D S T K N K L V V E A D I A N E T K E R R I V G E G V T V G D F S H . F S F E G S V


130 140 150 160 170 180D C L R Y W R S K A I D D N I V A R K Q R L L C G N L F H D L S V R V P L D S E E V I D T W L E M Q D A F R N - S P N FV N L F Y Y R S D A V R R N V P N - - P I Y M Q G R Q F H D I L M K V P L D N N D L I D T W E G T V K A I G S - T G A FV N M Y Y Y R S D A V R R N V P N - - P I Y M Q G R Q F H D I I M K V P L D N P D V I D T W E G T V K A V Q S - T G A FV N L F Y Y R S D A V R R N V P N - - P I Y M Q G R Q F H D I I M K V P L D N N D V I D T W E G T V K A L Q S - T G S FV N M Y Y F R S D A V R R N V P N - - P I Y M Q G R Q F H D I M M K V P L D N N D L I D T W E G F Q Q S I S G G G A N FV N L . Y Y R S D A V R R N V P N R K P I Y M Q G R Q F H D I . M K V P L D N N D V I D T W E G T V K A . S G T G F


190 200 210 220 230 240G D Y I K D V W L I G P L W N A L E Q T G Q S L D N I D V Y Y F S E V E G - - G E K S R I D F R F A G G G T G I V D S IN D W I R D F W F I G P A F T A L N E G G Q R I S R I E V N G L N T E S G P K G P V G V S R W R F S H G G S G M V D S IN D W I R D F W F I G P A F T A L N E G G Q R I S R I E V N S I G T Q G S D K G P V G V S R W R F S H G G S G V V D S IN D W I R E F W F I G P A F T A L N E G G Q R I S R I E V N S I G T Q S G E K G P V G V S R W R F S H G G S G I V D S IG D W I R E F W F I G P A F T A I N E G G Q R I S P I Q V N N F G V E S G E K G P V G V S R W K F S H A G S G I V D S IN D W I R D F W F I G P A F T A L N E G G Q R I S R I E V N . G T S G . K G P V G V S R W R F S H G G S G I V D S I


250 260 270 280 290 300S R W L E L F P I D G L G K P V N Q G G R V A R L Q G N F N A S V Q G I E V K L F V E L P G F S V P I E G G K R K V L NS R W A E L F P S D K L N R P A Q - - - - - - - V E A G F R S D S Q G I E V K V D G E F P G V S V D A G G G L R R I L NS R WM E L F P S D K L N R P A S - - - - - - - V E A G F R S D S Q G I E V K V D G E F P G V S V D A G G G L R R I L NS R W A E L F P S D K L N K P A S - - - - - - - V E A G F R S D S Q G I E V K V D G E F P G V S V D A G G G L R R I L NS R W A E L F P V D Q L N K P A S - - - - - - - I E G G F R S D S Q G I E V K V D G N L P G V S R D A G G G L R R I L NS R W A E L F P S D K L N K P A S Q G G R V A R V E A G F R S D S Q G I E V K V D G E F P G V S V D A G G G L R R I L N


310 320 330 340 350 360H P L V P L A H H G I A V N S E P A D L S I R F K V S I P K G K K F I E T A N S F E W D R V A E N V R V F T G G R Y K AH P L I P L V H H G M V G K F N N F N V D A Q L K V V L P K G Y K I R Y A A P Q Y R S Q N L E E Y R - - W S G G A Y A RH P L I P L V H H G M V G K F N D F T V D T Q L K I V L P K G Y K V R Y A A P Q F R S Q N L E E Y R - - W S G G A Y A RH P L I P L V H H G M V G K F N D F T V D T Q L K I V L P K G Y K V R Y A A P Q F R S Q N L E E Y R - - W S G G A Y A RH P L I P L V H H G M V G K F N D F T V D T Q L K V V L P K G Y K I R Y A A P Q F R S Q N L E E Y R - - W S G G A Y A RH P L I P L V H H G M V G K F N D F T V D T Q L K V V L P K G Y K . R Y A A P Q F R S Q N L E E Y R R V W S G G A Y A R


370 380 390 400 410 420W A E G I C K G S Y S P F D I F F GW V E H V C K G G V G Q F E I L Y A QW V E H V C K G G T G Q F E V L Y A QW V E H V C K G G T G Q F E V L Y A QW V E H V C K G G T G Q F E V L Y A QW V E H V C K G G T G Q F E V L Y A Q

Ligands to BChl a

450,000 550,000 650,000 750,000 850,000 950,000350,000

B. bchG-orf-csmA bchY

Figure 6S

A.

0 50000 100000 150000 200000 250000 300000 350000 400000

16S rRNA recA pscAB-fmoA

BAC M60-018 J19

BAC M60-018 J19

Figure 7S16S rRNA Alignment

GFP1 16S rRNACab. thermophilum 16S rRNA

10 20 30 40 50 60 70 80 90 100 110 120T C G A A C G G G A A A G T C C T T C G G G A C G A G T A C A G T G G C G C A C G G G T G A G T A A C G C G T G G G T T A T C T A

G A G A G T T T G A T C C T G G C T C A G A A T C A A C G C T G G C G G C A T G C C T A A C A C A T G C A A G T C G A A C G G G A A A G T C C T T C G G G A C G A G T A C A G T G G C G C A C G G G T G A G T A A C G C G T G G G T T A T C T AG A G A G T T T G A T C C T G G C T C A G A A T C A A C G C T G G C G G C A T G C C T A A C A C A T G C A A G T C G A A C G G G A A A G T C C T T C G G G A C G A G T A C A G T G G C G C A C G G G T G A G T A A C G C G T G G G T T A T C T A


130 140 150 160 170 180 190 200 210 220 230 240C C T C C G G G T G G G G A A T A A C T C G G G G A A A C T C G A G C T A A T A C C G C A T A A T A C G T - C T A C G T T A A A G C A G C A A T G C G C C T G G A G A T G A G C C T G C G T C C G A T T A G C T A G T T G G T G G G G T A A T GC C T C C G G G T G G G G A A T A A C T C G G G G A A A C T C G A G C T A A T A C C G C A T A A T A C G T T C T A C G T T A A A G C A G C A A T G C G C C T G G A G A T G A G C C T G C G T C C G A T T A G C T A G T T G G T G G G G T A A T GC C T C C G G G T G G G G A A T A A C T C G G G G A A A C T C G A G C T A A T A C C G C A T A A T A C G T T C T A C G T T A A A G C A G C A A T G C G C C T G G A G A T G A G C C T G C G T C C G A T T A G C T A G T T G G T G G G G T A A T G


250 260 270 280 290 300 310 320 330 340 350 360G C C C A C C A A G G C G A C G A T C G G T A G C C G G C C T G A G A G G G C G G T C G G C C A C A C T G G C A C T G A A A C A C G G G C C A G A C T C C T A C G G G A G G C A G C A G T G A G G A A T C T T G G G C A A T G G G C G A A A G CG C C C A C C A A G G C G A C G A T C G G T A G C C G G C C T G A G A G G G C G G T C G G C C A C A C T G G C A C T G A A A C A C G G G C C A G A C T C C T A C G G G A G G C A G C A G T G A G G A A T C T T G G G C A A T G G G C G A A A G CG C C C A C C A A G G C G A C G A T C G G T A G C C G G C C T G A G A G G G C G G T C G G C C A C A C T G G C A C T G A A A C A C G G G C C A G A C T C C T A C G G G A G G C A G C A G T G A G G A A T C T T G G G C A A T G G G C G A A A G C


370 380 390 400 410 420 430 440 450 460 470 480C T G A C C C A G C A A T G C C G C G T G A A C G A T G A A G T C C T T C G G G A T G T A A A G T T C A T A A G C A G G A G A A G A A C A C C A T G A C G G T A T C C T G T G T A A G C C C C G G C C A A C T A C G T G C C A G C A G C C G C GC T G A C C C A G C A A T G C C G C G T G A A C G A T G A A G T C C T T C G G G A T G T A A A G T T C A T A A G C A G G A G A A G A A C A C C A T G A C G G T A T C C T G T G T A A G C C C C G G C C A A C T A C G T G C C A G C A G C C G C GC T G A C C C A G C A A T G C C G C G T G A A C G A T G A A G T C C T T C G G G A T G T A A A G T T C A T A A G C A G G A G A A G A A C A C C A T G A C G G T A T C C T G T G T A A G C C C C G G C C A A C T A C G T G C C A G C A G C C G C G


490 500 510 520 530 540 550 560 570 580 590 600G T A A T A C G T A G G G G G C A A G C G T T G T T C G G A T T T A C T G G G C G T A A A G G G C G C G T A G G C G G C G C G T A A A G T C G G A C A T G A A A T C A T T A G G C T C A A C C T A A T G G C T G T G T C C G A T A C T T A C G CG T A A T A C G T A G G G G G C A A G C G T T G T T C G G A T T T A C T G G G C G T A A A G G G C G C G T A G G C G G C G C G T A A A G T C G G A C A T G A A A T C A T T A G G C T C A A C C T G A T G G C T G T G T C C G A T A C T T A C G TG T A A T A C G T A G G G G G C A A G C G T T G T T C G G A T T T A C T G G G C G T A A A G G G C G C G T A G G C G G C G C G T A A A G T C G G A C A T G A A A T C A T T A G G C T C A A C C T R A T G G C T G T G T C C G A T A C T T A C G Y


610 620 630 640 650 660 670 680 690 700 710 720G C T T G G A G T G C G G G A G A G G C A G C C G G A A T T C T C G G T G T A G C G G T G A A A T G C G T A G A T A T C G A G A G G A A C A C C G G T G A C G A A G G T G G G T T G C T G G A C C G A C A C T G A C G C T G A G G C G C G A A AG C T T G G A G T G C G G G A G A G G C A G C C G G A A T T C T C G G T G T A G C G G T G A A A T G C G T A G A T A T C G A G A G G A A C A C C G G T G A C G A A G G T G G G T T G C T G G A C C G A C A C T G A C G C T G A G G C G C G A A AG C T T G G A G T G C G G G A G A G G C A G C C G G A A T T C T C G G T G T A G C G G T G A A A T G C G T A G A T A T C G A G A G G A A C A C C G G T G A C G A A G G T G G G T T G C T G G A C C G A C A C T G A C G C T G A G G C G C G A A A


730 740 750 760 770 780 790 800 810 820 830 840G C T A G G G G A G C A A A C G G G A T T A G A T A C C C C G G T A G T C C T A G C C C T A A A C G A T G A A T A C T T G A T G T G C G A G T T A C T A G G C A C T C G C G T G T C G A A G C T A A C G C G A T A A G T A T T C C G C C T G G GG C T A G G G G A G C A A A C G G G A T T A G A T A C C C C G G T A G T C C T A G C C C T A A A C G A T G A A T A C T T G A T G T G C G A G T T A C T A G G C A C T C G C G T G T C G A A G C T A A C G C G A T A A G T A T T C C G C C T G G GG C T A G G G G A G C A A A C G G G A T T A G A T A C C C C G G T A G T C C T A G C C C T A A A C G A T G A A T A C T T G A T G T G C G A G T T A C T A G G C A C T C G C G T G T C G A A G C T A A C G C G A T A A G T A T T C C G C C T G G G


850 860 870 880 890 900 910 920 930 940 950 960G A G T A T G A C C G C A A G G T T G A A A C T C A A A G G A A T N G A C G G G G A C C C G C A C A A G C G G T G G A G C A T G T G G T T T A A T T C G A C G C A A C G C G A A G A A C C T T A C C T G A G T T C A A G A T A C G A T G A A G CG A G T A T G A C C G C A A G G T T G A A A C T C A A A G G A A T T G A C G G G G A C C C G C A C A A G C G G T G G A G C A T G T G G T T T A A T T C G A C G C A A C G C G A A G A A C C T T A C C T G A G T T C A A G A T A C G A T G A A G CG A G T A T G A C C G C A A G G T T G A A A C T C A A A G G A A T T G A C G G G G A C C C G C A C A A G C G G T G G A G C A T G T G G T T T A A T T C G A C G C A A C G C G A A G A A C C T T A C C T G A G T T C A A G A T A C G A T G A A G C


970 980 990 1000 1010 1020 1030 1040 1050 1060 1070 1080G C C G G A A T C G G C G T G G C C G G G C A A C C G G A G T C G T A T C A G G T G C T G C A T G G C T G T C G T C A G C T C G T G T C G T G A G A T G T T G G G T T A A G T C C C G C A A C G A G C G C A A C C C C T G T C G C C A G T T G CG C C G G A A T C G G C G T G G C C G G G C A A C C G G A G T C G T A T C A G G T G C T G C A T G G C T G T C G T C A G C T C G T G T C G T G A G A T G T T G G G T T A A G T C C C G C A A C G A G C G C A A C C C C T G T C G C C A G T T G CG C C G G A A T C G G C G T G G C C G G G C A A C C G G A G T C G T A T C A G G T G C T G C A T G G C T G T C G T C A G C T C G T G T C G T G A G A T G T T G G G T T A A G T C C C G C A A C G A G C G C A A C C C C T G T C G C C A G T T G C


1090 1100 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200C A C C A G T T C G G C T G A G C A C T C T G G C G A G A C C G C T G A T G A T G A A T C A G A G G A A G G T G G G G A T G A C G T C A A G T C C T C A T G G C C C T T A T G C T C A G G G C T A C A C A C G T G C T A C A A T G G T G A A T AC A C C A G T T C G G C T G A G C A C T C T G G C G A G A C C G C T G A T G A T G A A T C A G A G G A A G G T G G G G A T G A C G T C A A G T C C T C A T G G C C C T T A T G C T C A G G G C T A C A C A C G T G C T A C A A T G G T G A A T AC A C C A G T T C G G C T G A G C A C T C T G G C G A G A C C G C T G A T G A T G A A T C A G A G G A A G G T G G G G A T G A C G T C A A G T C C T C A T G G C C C T T A T G C T C A G G G C T A C A C A C G T G C T A C A A T G G T G A A T A


1210 1220 1230 1240 1250 1260 1270 1280 1290 1300 1310 1320A C A A A G A G A T G C A A A C C C G C G A G G G G G A G C G A A T C T C A A A A A T T T C A C C T C A G T T C G G A T T G C A G T C T G C A A C T C G A C T G C A T G A A G T C G G A A T C G C T A G T A A T C G C A G A T C A G C A C G C TA C A A A G A G A T G C A A A C C C G C G A G G G G G A G C G A A T C T C A A A A A T T T C A C C T C A G T T C G G A T T G C A G T C T G C A A C T C G A C T G C A T G A A G T C G G A A T C G C T A G T A A T C G C A G A T C A G C A C G C TA C A A A G A G A T G C A A A C C C G C G A G G G G G A G C G A A T C T C A A A A A T T T C A C C T C A G T T C G G A T T G C A G T C T G C A A C T C G A C T G C A T G A A G T C G G A A T C G C T A G T A A T C G C A G A T C A G C A C G C T


1330 1340 1350 1360 1370 1380 1390 1400 1410 1420 1430 1440G C G G T G A A T A C G T T C C C G G G T C T T G T A C A C A C C G C C C G T C A C A T C A C G A A A G T A G G C T G C A C T A G A A G T G G C T G A G C T A A C C C GG C G G T G A A T A C G T T C C C G G G T C T T G T A C A C A C C G C C C G T C A C A T C A C G A A A G T A G G C T G C A C T A G A A G T G G C T G A G C T A A C C C G C A A G G G A G G C A G G T T A C A A C G G T G T G G T C T A T G A T TG C G G T G A A T A C G T T C C C G G G T C T T G T A C A C A C C G C C C G T C A C A T C A C G A A A G T A G G C T G C A C T A G A A G T G G C T G A G C T A A C C C G C A A G G G A G G C A G G T T A C A A C G G T G T G G T C T A T G A T T


1450 1460 1470 1480 1490 1500 1510 1520 1530 1540 1550 1560

G G G G T G A A G T C G T A A C A A G G T A G C T G T A G G A G A A C C T G T G G C T G G A T C A C C T C C T TG G G G T G A A G T C G T A A C A A G G T A G C T G T A G G A G A A C C T G T G G C T G G A T C A C C T C C T T

Deinococcus radiodurans DR1105

Campylobacter jejuniThiomicrospira denitrificansWolinella succinogenesHelicobacter pylori

Gloeobacter violaceus PCC 7421Synechococcus sp. 2-3B’a(2-13)Synechococcus sp. JA-3-3AbSynechocystis sp. PCC 6803Synechococcus sp. PCC 7002Synechococcus sp. PCC 6301Nostoc sp. PCC 7120Nostoc punctiforme PCC 73102

Petrotoga mobilisThermotoga maritimaThermosipho melanesiensisFervidobacterium nodosum

Corynebacterium glutamicum

Streptomyces coelicolorNocardia farcinica

Brevibacterium linensArthrobacter aurescens

Streptococcus pyogenesStaphylococcus aureusListeria monocytogenesBacillus subtilisBacillus cereusBacillus anthracis

Stigmatella aurantiacaAnaeromyxobacter sp.Anaeromyxobacter dehalogensGeobacter lovleyiGeobacter sulfurreducensGeobacter metallireducensChloracidobacterium thermophilumSolibacter usitatus Ellin6076Acidobacteria sp. Ellin345

Aquifex pyrophilusAquifex aeolicusDeinococcus radioduransThermus thermophilusThermus aquaticus

Erythrobacter litoralisBlastochloris viridisRhodopseudomonas palustrisBradyrhizobium japonicumRoseobacter denitrificansRhodobacter sphaeroidesRhodobacter capsulatusShewanella oneidensisProteus vulgarisShigella flexneriEscherichia coliHalorhodospira halophilaDechloromonas aromaticaBordetella pertussisBordetella parapertussis

Dehalococcoides ethenogenesHerpetosiphon aurantiacusChloroflexus aurantiacus J-10-flRoseiflexus sp. RS1Roseiflexus castenholziiThermoanaerobacter tengcongensisThermoanaerobacter ethanolicusClostridium novyiClostridium botulinumClostridium acetobutylicumPelotomaculum thermopropionicumCarboxydothermus hydrogenoformansHeliobacterium modesticaldumDesulfitobacterium hafniense

Chloroherpeton thalassium

Chlorobium phaeobacteroides BS1Chlorobium tepidumChlorobaculum parvum DSM 263

Chlorobium ferooxidansPelodictyon luteolum DSM 273Chlorobium phaeovibriodes DSM 265Chlorobium chlorochromatiiPelodictyon phaeoclathratiformeProsthecochloris aesturarii DSM 271Chlorobium phaeobacteroides DSM 266Chlorobium limicola DSM 245Chlorobium

Cytophaga hutchinsoniiPorphyromonas gingivalisBacteriodes thetaiotaomicronBacteriodes fragilis

95

92

83

81

99

99

73

99

99

70

73

94

100

70

99

100

79

9696

75

96 99

99

89

99

70

77

92

80

99

99

99

99

99

99

91

77

99

95

84

99

99

99

97

100

71

99

88

Chloroflexi

Cyanobacteria

Chlorobi

Acidobacteria

β-Proteobacteria

α-Proteobacteria

γ-Proteobacteria

Aquificae

Thermotogae

Deinococcus-Thermus

δ-Proteobacteria

Actinobacteria

Firmicutes (“Bacilli”)

Bacteriodetes

Firmicutes (“Clostridia”)

ε-Proteobacteria

Figure 8S

Cab. thermophilum RecASolibacter usitatus RecAAcidobacteria sp. Ellin345 RecA RecADehalococcoides ethenogenes RecAHerpetosiphon aurantiacus RecAChloroflexus aggregans RecACfx. aurantiacus J-10-fl RecARoseiflexus sp. RS1 RecARoseiflexus castenholzii RecAThe. tengcongensis RecAThe. ethanolicus RecAClostridium novyi RecAClostridium botulinum RecAClostridium acetobutylicum RecAPel. thermopropionicum RecACar. hydrogenoformans RecAHb. modesticaldum RecADesulfitobacterium hafniense RecAStreptococcus pyogenes RecAStaphylococcus aureus RecAListeria monocytogenes RecABacillus subtilis RecABacillus cereus RecABacillus anthracis RecAAquifex pyrophilus RecAAquifex aeolicus RecADeinococcus radiodurans RecAThermus thermophilus RecAThermus aquaticus RecACorynebacterium glutamicum RecABrevibacterium linens RecAArthrobacter aurescens RecAStreptomyces coelicolor RecANocardia farcinica RecAErythrobacter litoralis RecABlastochloris viridis RecARhodopseudomonas palustris RecABradyrhizobium japonicum RecARoseobacter denitrificans RecARhodobacter sphaeroides RecARhodobacter capsulatus RecAShewanella oneidensis RecAProteus vulgaris RecAShigella flexneri RecAEscherichia coli RecAHalorhodospira halophila RecADechloromonas aromatica RecABordetella pertussis RecABordetella parapertussis RecAStigmatella aurantiaca RecAAnaeromyxobacter sp. RecAAnaeromyxobacter dehalogens RecAGeobacter lovleyi RecAGeobacter sulfurreducens RecAGeobacter metallireducens RecACampylobacter jejuni RecAThiomicrospira denitrificans RecAWolinella succinogenes RecAHelicobacter pylori RecAChloroherpeton thalassium RecAChlorobium ferooxidans RecAPelodictyon luteolum RecAChlorobium vibrioforme RecAChlorobium chlorochromatii RecAPld. .phaeoclathratiforme RecAProsthecochloris aesturarii RecAChlorobium phaeobacteroides RecAChlorobium limicola RecAChlorobium phaeobacteroides BS1Chlorobium tepidum RecAChlorobaculum parvum RecACytophaga hutchinsonii RecAPorphyromonas gingivalis RecABacteriodes thetaiotaomicron RecABacteriodes fragilis RecAPetrotoga mobilis RecAThermotoga maritima RecAThermosipho melanesiensis RecAFervidobacterium nodosum RecAGloeobacter violaceus RecASynechococcus sp. JA-2-3B'a(2-13)Synechococcus sp. JA-3-3Ab RecASynechocystis sp. PCC 6803 RecASynechococcus sp. PCC 7002 RecASynechococcus sp. PCC 6301 RecANostoc sp. PCC 7120 RecANostoc punctiforme PCC 73102 RecADeinococcus radiodurans DR1105

1 60 1 59 1 61 1 58 1 58 1 0 1 60 1 60 1 60 1 57 1 56 1 57 1 61 1 57 1 57 1 57 1 56 1 59 1 71 1 57 1 56 1 56 1 56 1 56 1 63 1 60 1 70 1 57 1 57 1 70 1 68 1 59 1 58 1 60 1 68 1 56 1 70 1 69 1 70 1 70 1 70 1 59 1 59 1 59 1 59 1 59 1 58 1 66 1 66 1 61 1 61 1 61 1 57 1 58 1 58 1 57 1 57 1 59 1 59 1 71 1 71 1 70 1 71 1 72 1 70 1 70 1 70 1 70 1 70 1 71 1 72 1 66 1 66 1 72 1 74 1 64 1 60 1 60 1 59 1 74 1 56 1 56 1 60 1 61 1 63 1 61 1 61 1 149

M T D T R Q E R N K A I D A A I A S L E K Q F G K G V V M R L G E R - - - - - - - - - - - Q A L E V P A I S T T C L S L D A A I G I G G V P RM D E K E R S R A L E T A L G Q I E K Q F G K G S I L R L G S K D - - - - - - - - - - A I V P V S V I S T G S I S L D A A L G V G G F P R

M S D E R T E R G R S L E L A L S Q I E K Q F G K G S I M K L G A K G - - - - - - - - - - A I V P I S V I S T G A I S F D A A L G T G G F P RM T T E K D K A L E L T V G I I E K R F G K G S I M K L S D P T F - - - - - - - - - - R Q T V E F I P T S S L A L D I A L G V G G I P R

M A I S P E K E K A L A A A L S Q I D R Q F G K G A I M R M S D Q - - - - - - - - - - - - R L S I E A I P T G S I A L D L A L G V G G I P R

M A I T P E K E K A L A A T M A Q I D R K F G K G S I M K M G E A S S - - - - - - - - - - R V A I E A I P T G S I A L D I A L G I G G V P RM A L S P E K E K A L A A A M S Q I D R K Y G K G S I M R M G E V S S - - - - - - - - - - K L A I E V I P T G S I A L D I A L G V G G V P RM A L S P E K E K A L A A A M S Q I D R K Y G K G S I M R M G E A S S - - - - - - - - - - K L A I E V I P T G S I A L D I A L G V G G V P R

M M I E K Q K A L E M A I S Q I E R Q F G K G - A I M R L G D T A - - - - - - - - - - K L N V E V I P T G S L E L D I A L G V G G V P RM I E K Q K A L E M A I S Q I E R Q F G K G - S I M R L G D T A - - - - - - - - - - K L N V E V I P T G S L E L D I A L G V G G V P R

M N N E K M K A L Q A A I T Q I E K Q F G K G - S V M K L G E E H - - - - - - - - - - V L N V E A I S T G S L S L D I A L G I G G I P RM A N K V N P E K L K A I E A A M G Q I E K Q F G K G - S I M K L G E D S - - - - - - - - - - I L N V E S I S T G S L D L D I A L G I G G V P R

M D N E K L K A I D A A M S Q I E K Q F G K G - A I M K L G E Q D - - - - - - - - - - I L N I D A I S T G C L S L D I A L G I G G V P RM S D R Q K A L E L A L M Q I E R Q F G K G S I M K L G E A S A - - - - - - - - - - K L N V E V I P T G A L A L D I A L G V G G V P RM N D K L K A L E L T I S Q I E K Q F G K G S I M R L G E N T G - - - - - - - - - - K F Q V E V I P T G S L A L D L A L G V G G V P RD N K L Q A L Q Q A L N S I E K A F G K G S I M R L G E A S A - - - - - - - - - - K L L V E V I P T G S I A L D M A L G I G G V P R

M A G N D K L K A L D M A L S Q I E K Q F G K G S I M R L G E A S A - - - - - - - - - - K M A V E V I P T G C L A I D I A L G V G G V P RM A K K L K K N E E I T K K F G D E R R K A L D D A L K N I E K D F G K G A V M R L G E R A E - - - - - - - - - - - Q K V Q V M S S G S L A L D I A L G A G G Y P K

M D N D R Q K A L D T V I K N M E K S F G K G A V M K L G D N I G - - - - - - - - - - - R R V S T T S T G S V T L D N A L G V G G Y P KM N D R Q A A L D Q A L K Q I E K Q F G K G S I M K L G E H S D - - - - - - - - - - - Q N I S T I S S G S L A L D I A L G V G G Y P RM S D R Q A A L D M A L K Q I E K Q F G K G S I M K L G E K T D - - - - - - - - - - - T R I S T V P S G S L A L D T A L G I G G Y P RM S D R Q A A L D M A L K Q I E K Q F G K G S I M K L G E Q A E - - - - - - - - - - - R K V S T V S S G S L A L D V A L G V G G Y P RM S D R Q A A L D M A L K Q I E K Q F G K G S I M K L G E Q A E - - - - - - - - - - - R K V S T V S S G S L A L D V A L G V G G Y P R

M A R V S E N L S E K M K A L E V A L S S I E K R F G K G A V M P L K A V E T - - - - - - - - - - - V E V E T I P T G S I S L D I A T G V G G I P KM S E N L S E K K K A L E V A L S N I E K R F G K G A V M P L K S V E K - - - - - - - - - - - V Q V E T I P T G S L A L D I A T G V G G I P K

M S K D A T K E I S A P T D A K E R S K A I E T A M S Q I E K A F G K G S I M K L G A E S K - - - - - - - - - - - L D V Q V V S T G S L S L D L A L G V G G I P RM D E S K R K A L E N A L K A I E K E F G K G A V M R L G E M P K - - - - - - - - - - - Q Q V D V I P T G S L A L D L A L G I G G I P RM D E S K R K A L E N A L K A I E K E F G K G A V M R L G E M P K - - - - - - - - - - - Q Q V D V I P T G S L A L D L A L G I G G I P R

M A P K K T A T K A T A A K G N D R Q K A L D A A L A L I E K D F G K G A V M R L G - D E N - - - - - - - - - - R P P I Q T I S S G N T A I D I A L G I G G F P RM A R T P K N L Q I P T G G D K S K A L D A A L G Q I D R N Y G K G A I M R L G - E G V - - - - - - - - - - R E P I A C I P T G S I A L D I A L G I G G L P R

M A A T P D R E K A L E A A L A Q I D K Q F G K G S I M R L G - D D T - - - - - - - - - - R A P I E V I P T G S I A L D V A L G I G G L P RM A G T D R E K A L D A A L A Q I E R Q F G K G A V M R M G - D R T - - - - - - - - - - N E P I E V I P T G S T A L D V A L G V G G I P R

M A P Q A Y D R D K A L E L A L A Q V E K S F G K G A V M R L G - E E A - - - - - - - - - - R Q P I S V I P T G S I A L D V A L G I G G L P RM S A N L K L V E K E N N V D R Q K A L D A A L A Q I D R A F G K G S A M K L G S K E - - - - - - - - - - - A M N V E A I S T G S L G L D I A L G I G G L P K

M D K T K A L D A A L T Q I E R A F G K G S I M R L G K S G A - - - - - - - - - - A L E V E T I S T G S L G L D I A L G I G G L P RM A A P T A L R I V E G S S M D K S K A L S A A L S Q I E R Q F G K G S V M R L G K N D R - - - - - - - - - - S M D I E T I S S G S L G L D I A L G V G G L P KM S A T A L R I V E G S S M D K S K A L A A A L S Q I E R Q F G K G S V M K L G K N D R - - - - - - - - - - S M D V E A V S S G S L G L D I A L G I G G L P K

M A T A D L L S M N S K K S A D K Q K A L D S A L A Q I E R Q F G K G S I M K L G A E G A - - - - - - - - - - V Q D I Q A S S T G S L G L D I A L G I G G L P MM A T A N L L D L N G R R E M D K A K A L E S A L A Q I E R Q F G K G S I M K L G A N S P - - - - - - - - - - V M E I E A T S T G S L G L D I A L G I G G L P KM A T T G L F E M N D K G K A D K Q K A L E C A L A Q I E R Q F G K G S I M K L G G D T P - - - - - - - - - - P P E I E A T S T G S L G L D I A L G I G G L P K

M K V D P N K E K A L A A V L S Q I E K Q F G K G S I M K L G E D - - - - - - - - - - - R S M D V E T I S T G S L S L D V A L G A G G L P MM A I D E N K Q K A L A A A L G Q I E K Q F G K G S I M R L G E D - - - - - - - - - - - R S M D V E T I S T G S L S L D I A L G A G G L P MM A I D E N K Q K A L A A A L G Q I E K Q F G K G S I M R L G E D - - - - - - - - - - - R S M D V E T I S T G S L S L D I A L G A G G L P MM A I D E N K Q K A L A A A L G Q I E K Q F G K G S I M R L G E D - - - - - - - - - - - R S M D V E T I S T G S L S L D I A L G A G G L P M

M D D D R K K A L G A A L G Q I E K Q F G K G A V M R M G D A R - A - - - - - - - - V R G D V K S I S T G S L A L D V A L G I G G L P RM D D N K A K A L A A A L Q Q I E K Q F G K G S I M K M G D A - - E - - - - - - - - I D E G I Q V V S T G S L G L D I A L G V G G L P R

M D D K T S K A A A A E K A K A L A A A L S Q I E K Q F G K G S I M R Y G D N - - E - - - - - - - - V E H D I Q V V S T G S L G L D I A L G V G G L P RM D D K T S K A A A A E K A K A L A A A L S Q I E K Q F G K G S I M R Y G D N - - E - - - - - - - - V E H D I Q V V S T G S L G L D I A L G V G G L P R

M A V N S E K E K A I E L A M S A V E R Q F G K G S I M R L G K D E P - - - - - - - - - L T R D I Q A I S T G S I S L D I A L G V G G V P KM A V A P E K E K A I E L A V S S I E K A F G K G S I M R L G N E E A - - - - - - - - - L V K D V Q A V S T G S V S L D I A L G V G G F P RM P V G P E K E K A I E L A V A S I E K A F G K G S I M R L G N E D A - - - - - - - - - L V K D V Q A V S T G A I S L D I A L G V G G F P R

M S D K N K A I E L A L S Q I E K Q F G K G A I M R L G N D E - - - - - - - - - - V L P G V E S I S T G A I S L D L A L G V G G V P RM T Q E R E K A I E L A L S Q I E K Q F G K G A I M R L G A D E - - - - - - - - - - A L P D V A A I P T G S L S L D L A L G V G G V P RM T Q E R E K A I E L A L S Q I E K Q F G K G A I M R L G A D E - - - - - - - - - - A L P D I D A I P T G A L S L D I A L G V G G V P RM D D N K R K S L D A A L K S L D K T F G K G T I L R L G - D K E - - - - - - - - - - V E Q I D S I G T G S V G L D L A L G I G G V P KM D A N K Q K S L D L A M K Q I D K A F G K G A L M R L G - D K E - - - - - - - - - - I M P I E S I S T G S L G L D L A L G I G G I P Q

M A L D E N K R K A I D L A I K Q I D K A F G K G A L V R L G - E K P - - - - - - - - - - V E K I D S I S T G S L G L D I A L G I G G I P QM A I D E D K Q K A I S L A I K Q I D K V F G K G A L V R L G - D K Q - - - - - - - - - - V E K I D S I S T G S L G L D L A L G I G G V P K

M A K E K A S S Q E Q E Q K V K D E K L K Q L N L A V E S L E K Q F G K G A I M R L G - D D A - - - - - - - - - - V V Q V P V V S T G S I S L D Y A L G V G G L P KM D T Q K I E Q S V P P V V D P A K L K Q L N L A V D A L E K Q F G K G A I M R L G D S S A - - - - - - - - - - V M Q V L S I S T G S M T L D F A L G V G G L P RM A T P R P A E T A P G V D P A K L K Q L N L A V E A L E K Q F G K G A I M R L G D D S A - - - - - - - - - - V M Q V Q S I S T G S M T L D F A L G V G G L P R

M D T T S K K E K K V P G I D P A K L K Q L D L A V D S L E K Q F G K G A I M R L G D D S A - - - - - - - - - - V M Q V Q A I S T G S M T L D F A L G V G G L P RM T M D N P K V E Q A G H A V D S A K L K Q L N L A V D A L E K Q F G K G T I M R M G D G S A - - - - - - - - - - G L T V Q A I S T G S M A L D F A L G V G G L P R

M D T Q K I E K K T V T V D P A K L K Q L N L A V E A L E K Q F G K G A I M R L G D D S A - - - - - - - - - - G M K V Q S I S T G S M T L D F A L G V G G L P RM D T Q K I E K K T V T V D P A K L K Q L N L A V E A L E K Q F G K G A I M R L G D D S A - - - - - - - - - - G M K V Q S I S T G S M T L D F A L G V G G L P RM D N I K T E Q K S D L V D P A K L K Q L N L A V D A I E K Q F G K G A I M R L G D D S A - - - - - - - - - - G M H V Q A I S T G S M T L D F A L G V G G L P RM D N I K T E Q K S D L V D P A K L K Q L N L A V D A I E K Q F G K G A I M R L G D D S A - - - - - - - - - - G M H V Q A I S T G S M T L D F A L G V G G L P RM D T K K N V Q E K K T A D P A K L K Q L S L A V E T L E K Q F G K G A I M C L G D Q G A - - - - - - - - - - I I P V Q S V S T G S I A L D F A L G V G G F P R

M E K E A T P Q Q Q P P V V D P A R L K Q L N L A I E T L E K Q F G K G A I M R L G D D S A - - - - - - - - - - V M H V Q V I S T G S M A L D Y A L G V G G L P RM D K E N S K Q T A Q P P V V D P A R L K Q L N L A I E T L E K Q F G K G S I M R L G D D S A - - - - - - - - - - V M H V K A I S T G S M A L D Y A L G V G G F P R

M S K S T S T P N P Q N E K L K A L Q L T I D K L E K T Y G K G T V M K L S - D E V - - - - - - - - - - V M D V P V I S T G S L G L D I A L G I G G L P KM A E E K I P T V Q D E K K L Q A L R M A T E K I E K T F G K G A I M N M G - A N T - - - - - - - - - - V E D V S V I P S G S I G L D L A L G V G G Y P R

M A K K D E L N F E T D N - K M A S S E K L K A L Q A T M E K I E K N F G K G S I M K M G - D D S - - - - - - - - - - V E Q V E V I P T G S I A L N V A L G V G G Y P RM A K K E D E L N F E T D N N K M A S S E K L K A L Q A A M D K I E K S F G K G S I M K M G - E E V - - - - - - - - - - V E Q V E V I P T G S I A L N A A L G V G G Y P R

M A K N D S K D A N K E E L L E K L V K E L E K N H G T G S V M L M G K G L D - - - - - - - - - - N S N L A V V P S G C L S L D I A L G V G G Y P RM P E E K Q K K S V L E K A L K R I E E N F G K G S I M I L G D E T Q - - - - - - - - - - V Q P V E V I P T G S L A I D I A T G V G G Y P RM S D N K Q K K E V L K K A I G K I E K T F G K G S I M F L G E E N A - - - - - - - - - - V Q N I E T I S S G S V A I D I A S G V G G Y P RM S D E M K K D V L K K A I S K I E K T Y G K G S I M V L G E G N L - - - - - - - - - - V Q E I D V I P S G S L A I D I A S G V G G Y P R

M A R T T D D S K K A A P A A G T A D E A Q K Q K A L K M V L T Q I K R N F G E G A I M R L G - E N T - - - - - - - - - - R I R V E T V P S G A I T L D L A L G G G L P - RM K S E K R K A L E Q V L S Q I E R N F G K G A I M R L G - D A T - - - - - - - - - - Q M R V E T I P S G A I T L D L A L G G G Y P - KM K S E K R K A L D Q V L G Q I E R N F G K G A I M R L G - D A A - - - - - - - - - - Q M R V E T I P S G A I T L D L A M G G G Y P - K

M A S T N I S D R E K A L N A A L A Q I E R S F G K G A I M R L G - D A T - - - - - - - - - - Q M R V E T I S T G A L T L D L A L G G G L P - KM S A I S N N P D K E K A L N L V L N Q I E R N F G K G A I M R L G - D A A - - - - - - - - - - Q M K V A T I P S G A L T L D Q A M G G G F P - R

M A S K S D A I A P E K E K A L N L V L S Q I E R N F G K G A I M R L G - D A A - - - - - - - - - - R L R V E T I S T G A L T L D L A L G G G L P - KM A I N T D T S G K Q K A L T M V L N Q I E R S F G K G A I M R L G - D A T - - - - - - - - - - R M R V E T I S T G A L T L D L A L G G G L P - KM A I N T D T S G K Q K A L T M V L N Q I E R S F G K G A I M R L G - D A T - - - - - - - - - - R M R V E T I S S G A L T L D L A L G G G L P - K

M E R V A V L F R R T C N S E N C S K G R R P L R E R P L A G L F F C S L P A P L S I P C L S L P G S N P G D F V P K V K T N Y I C N S C G Y Q S A K P L G R C P N C Q A W N S F E E E V P T A S T S G K S G R G G L G G Y G G V K G G K L T P L S T V G R R E E P R T P S G I P E L D R V L G G G L V - A

Cab. thermophilum RecASolibacter usitatus RecAAcidobacteria sp. Ellin345 RecADehalococcoides ethenogenes RecAHerpetosiphon aurantiacus RecAChloroflexus aggregans RecACfx. aurantiacus J-10-fl RecARoseiflexus sp. RS1 RecARoseiflexus castenholzii RecAThe. tengcongensis RecAThe. ethanolicus RecAClostridium novyi RecAClostridium botulinum RecAClostridium acetobutylicum RecAPel. thermopropionicum RecACar. hydrogenoformans RecAHb. modesticaldum RecADesulfitobacterium hafniense RecAStreptococcus pyogenes RecAStaphylococcus aureus RecAListeria monocytogenes RecABacillus subtilis RecABacillus cereus RecABacillus anthracis RecAAquifex pyrophilus RecAAquifex aeolicus RecADeinococcus radiodurans RecAThermus thermophilus RecAThermus aquaticus RecACorynebacterium glutamicum RecABrevibacterium linens RecAArthrobacter aurescens RecAStreptomyces coelicolor RecANocardia farcinica RecAErythrobacter litoralis RecABlastochloris viridis RecARhodopseudomonas palustris RecABradyrhizobium japonicum RecARoseobacter denitrificans RecARhodobacter sphaeroides RecARhodobacter capsulatus RecAShewanella oneidensis RecAProteus vulgaris RecAShigella flexneri RecAEscherichia coli RecAHalorhodospira halophila RecADechloromonas aromatica RecABordetella pertussis RecABordetella parapertussis RecAStigmatella aurantiaca RecAAnaeromyxobacter sp. RecAAnaeromyxobacter dehalogens RecAGeobacter lovleyi RecAGeobacter sulfurreducens RecAGeobacter metallireducens RecACampylobacter jejuni RecAThiomicrospira denitrificans RecAWolinella succinogenes RecAHelicobacter pylori RecAChloroherpeton thalassium RecAChlorobium ferooxidans RecAPelodictyon luteolum RecAChlorobium vibrioforme RecAChlorobium chlorochromatii RecAPld. phaeoclathratiforme RecAProsthecochloris aesturarii RecAChlorobium phaeobacteroides RecAChlorobium limicola RecAChlorobium phaeobacteroides BS1Chlorobium tepidum RecAChlorobaculum parvum RecACytophaga hutchinsonii RecAPorphyromonas gingivalis RecABacteriodes thetaiotaomicron RecABacteriodes fragilis RecAPetrotoga mobilis RecAThermotoga maritima RecAThermosipho melanesiensis RecAFervidobacterium nodosum RecAGloeobacter violaceus RecASynechococcus sp. JA-2-3B'a(2-13)Synechococcus sp. JA-3-3Ab RecASynechocystis sp. PCC 6803 RecASynechococcus sp. PCC 7002 RecASynechococcus sp. PCC 6301 RecANostoc sp. PCC 7120 RecANostoc punctiforme PCC 73102 RecADeinococcus radiodurans DR1105

61 170 60 169 62 171 59 168 59 168

1 108 61 170 61 170 61 170 58 167 57 166 58 167 62 171 58 167 58 167 58 167 57 166 60 169 72 181 58 167 57 166 57 165 57 166 57 166 64 173 61 170 71 180 58 167 58 167 71 180 69 178 60 169 59 168 61 170 69 178 57 166 71 180 70 179 71 180 71 180 71 180 60 169 60 169 60 169 60 169 60 169 59 168 67 176 67 176 62 171 62 171 62 171 58 167 59 168 59 168 58 167 58 167 60 169 60 169 72 181 72 181 71 180 72 181 73 182 71 180 71 180 71 180 71 180 71 180 72 181 73 182 67 176 67 176 73 182 75 184 65 174 61 170 61 170 60 169 75 184 57 166 57 166 61 170 62 171 64 173 62 171 62 171

150 299

G R I V E I Y G P E S G G K T T L A L H V A A E A Q R T G G N V A F I D A E H A L D P G Y A A K L G V D I D N L F V S Q P D N - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E A L V R S A A F D L V V V D S V A A L V P K A E L D G - - - - - D M G D A L P G L Q AG R I N E I F G P E S S G K T T I A L Q V V A E A Q K A G G I A A F I D V E H A L D P T Y A R K L G V D V D N L L V S Q P D Y - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I T A H L I A S G Q I D V L V V D S V A A L V P K S E L D G - - - - - E M G D S H M G V Q AG R V V E I F G P E S S G K T T I A L Q V I A E A Q K A G G M A A F V D A E H A L D P T Y A R K L G V D V E N L L V S Q P D Y - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I T E A L V S S K A I D I L V V D S V A A L V P K A E L D G - - - - - E M G D S H M G L Q AG R I S E I F G P E G S G K T T L A Q H I I A Q A Q K M G E K A A Y I D V E H A L D P K Y A S T C G V N L D E L L I S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - E A L G I A E E L V R S T A I G V I V I D S V A A L V P K A E I E G - - - - - D M G D S H V G L Q AG R V I E I Y G P E S S G K T T L T Q H I V A E T Q K M G G V A A F I D A E H A F D P V Y A A N C G V N V E D L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I C E T L V R S G A I D V I V V D S V A A L V P R A E I E G - - - - - D M G D S H V G L Q A

M I E I F G P E S S G K T T L A Q H I I A E A Q K M G G V G A F I D A E H A F D P V Y A A R C G V N I S D L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I C E M L V R S N A V D V I V I D S V A A L V P R A E I E G - - - - - D M G D S M P G M Q AG R V V E I F G P E S S G K T T L A Q H I I A E A Q K M G G V C A F I D A E H A F D P V Y A A R C G V N I D D L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I C E M L V R S N A I D V I V I D S V A A L V P R A E I E G - - - - - E M G D S M P G M Q AG R V V E I Y G P E S S G K T T L A Q H I I A E A Q K M G G I A A F I D A E H A F D P V Y A A R C G V D V N N L L V S Q P D Y - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I C E A L V R S N A V D V V V V D S V A A L V P R A E I E G - - - - - D M G D S L P G L Q AG R V V E I Y G P E S S G K T T L A Q H I I A E A Q K M G G V A A F I D A E H A F D P V Y A K R C G V D V D N L L V S Q P D Y - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I C E T L V R S N A V D V V V V D S V A A L V P R A E I E G - - - - - D M G D S L P G L Q AG R I I E I F G P E S S G K T T L A L H M I A E A Q K I G G T G A F I D A E H A L D P V Y A K N L G V N I D D L L V A Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E A L V R S G A V D I I V I D S V A A L V P K A E I E G - - - - - E M G D S H V G L Q AG R I I E I F G P E S S G K T T L A L H M I A E A Q K M G G T G A F I D A E H A L D P V Y A K N L G V N I E D L L V A Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E A L V R S G A V D I I V I D S V A A L V P K A E I D G - - - - - E M G D S H V G L Q AG R I I E I F G P E S S G K T T V A L H I I A E A Q K S G G T A A F I D A E H A L D P V Y A K A L G V D I D N L I V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I C E A L V R S G A I D V I V V D S V A A L V P K A E I E G - - - - - E M G D S H V G L Q AG R I I E I F G P E S S G K T T V A L H I L A E A Q K I G G A A A F I D A E H A L D P S Y A R N L G V D I D N L I V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I T E A L V R S G A V D V I V V D S V A A L V P R A E I E G - - - - - E M G D S H V G L Q AG R V V E I Y G P E S S G K T T V A L H I L A E A Q K K G G A A A F I D A E H A L D P Q Y A R A L G V D I D N L V V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E A L V R S G A I D V I V V D S V A A L V P K A E I E G - - - - - E M G D S H V G L Q AG R V I E I F G P E S S G K T T V A L H V I A E A Q K M G G T A A F V D A E H A L D P V Y A K N L G V D I E N L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E A L V R S G A V D V I V V D S V A A L V P R A E I E G - - - - - E M G D A H V G L Q AG R V V E I F G P E S S G K T T V A L H I I A E A Q K M G G I A A F I D A E H A L D P V Y A Q K L G V D I E N L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E S L V R S G A I D V I V I D S V A A L V P R A E I E G - - - - - E M G D A H V G L Q AG R I V E V Y G P E S S G K T T V A L H I I A E A Q K M G G M A A F I D A E H A L D P V Y A R N L G V D I D N L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I C E A L V R S G A I D V V V I D S V A A L V P K A E I D G - - - - - E M G D A H V G L Q AG R V I E I Y G P E S S G K T T V A L H I A A E A Q K L G G V A A F I D A E H A L D P V Y A K A L G V N I D N L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I C E A L V R S G A I D V I I I D S V A A L V P R A E I E G - - - - - E M G D S H V G L H AG R I I E I Y G P E S S G K T T V A L H A V A Q A Q K E G G I A A F I D A E H A L D P A Y A A A L G V N I D E L L L S Q P D S - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q G L E I A G K L I D S G A V D L V V V D S V A A L V P R A E I D G - - - - - D I G D S H V G L Q AG R I I E I Y G P E S S G K T T V A L H A I A E V Q S N G G V A A F I D A E H A L D P E Y A Q A L G V D I D N L Y L S Q P D H - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q G L E I A E A F V R S G A V D I V V V D S V A A L T P K A E I E G - - - - - E M G D T H V G L Q AG R I I E V Y G P E S S G K T T V A L H A I A E V Q A Q G G T A A F I D A E H A L D P A Y A K N L G V N I D E L L L S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E A L V R S G A V D M L V I D S V A A L V P R A E I E G - - - - - E M G D A H V G L Q AG R I I E V Y G P E S S G K T T V A L H A I A E V Q Q Q R - T S A F I D A E H A L D P V Y A Q K L G V N I E E L L L S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E A L V R S G A V D I V V V D S V A A L V P K A E I E G - - - - - D M G D S H V G L Q AG R I I E I Y G P E S S G K T T V S L H A I A E V Q R Q G G Q A A F I D A E H A M D P V Y A Q K L G V N I D E L L L S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q G L E I A E A L V R S G A V D I I V I D S V A A L V P K A E I E G - - - - - D M G D S H V G L Q AG R I I E I Y G P E S S G K T T V S L H A I A E V Q R Q G G Q A A F I D A E H A M D P V Y A Q K L G V N I D E L L L S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q G L E I A E A L V R S G A V D I I V I D S V A A L V P K A E I E G - - - - - D M G D S H V G L Q AG R I T E I F G V E S S G K T T L A L H V I A E A Q K R G G V A V F I D A E H A L D P K Y A K K L G V D V D N L Y I S Q P D Y - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E S L I N S G A V D V I V V D S V A A L V P K D E L E G - - - - - E M G E A Q V G K Q AG R I T E I F G V E S S G K T T L A L H V I A E A Q K R G G V A V F I D A E H A L D P K Y A K K L G V D V E N L Y I S Q P D Y - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E S L I N S G A V D V I V V D S V A A L V P K D E L E G - - - - - E M G E A Q V G K Q AG R I T E I Y G P E S G G K T T L A L A I V A Q A Q K A G G T C A F I D A E H A L D P V Y A R A L G V N T D E L L V S Q P D N - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I M E L L V R S G A I D V V V V D S V A A L T P R A E I E G - - - - - D M G D S L P G L Q AG R I V E I Y G P E S G G K T T L G L T I I A Q A Q R R G G V A A F V D A E H A L D P L Y A Q R L G V Q V E D L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I V E L L A R S G A V D V I V V D S V A A L V P R A E I E G - - - - - E M G D Q H V G L Q AG R I V E I Y G P E S G G K T T L A L T I I A Q A Q R R G G V A A F V D A E H A L D P L Y A Q R L G V Q V E D L L V S Q P E T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I V E L L A R S G A V D V I V V D S V A A L V P R A E I E G - - - - - E M G D Q H V G L Q AG R I V E V Y G P E S S G K T T V A L H A I A Q A Q K A G G I A A F I D A E H A L D P D Y A R K L G V D T D A L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A D M L V R S G A I D I I V I D S V A A L T P K A E I E G - - - - - E M G D S H V G L Q AG R V V E I Y G P E S S G K T T V A L H A V A N A Q K T G G I A A F I D A E H A L D P E Y A K K L G V D T D Q L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A D M L I R S G A L D V I V I D S V A A L V P K A E I E G - - - - - E M G D S H V G L Q AG R V V E I Y G P E S S G K T T V A L H A V A N A Q R A G G I A A F I D A E H A L D P D Y A A K L G V D T D A L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I M D M L V G S G S L D I V V I D S V A A L V P R A E I E G - - - - - E M G D S H V G L Q AG R V V E V Y G P E S S G K T T L T L H A V A N A Q K A G G Q V A F V D A E H A L D P E Y A K K L G V D I D N L I L S Q P D N - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I V D M L V R S G A L D L I V I D S V A A L V P R A E I E G - - - - - E M G D S H V G L Q AG R I V E I Y G P E S S G K T T V A L H A V A N A Q A A G G V A A F I D A E H A L D P D Y A R K L G V D T D A L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A D M L V R S G A I D I I V I D S V A A L V P R A E I E G - - - - - E M G D S H V G L Q AG R V I E V Y G P E S S G K T T L A L H A I A E A Q K A G G T A A F V D A E H A L D P V Y A K A L G V D I D E L I V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I T D T L V R S N A V D I L V V D S V A A L V P R A E I E G - - - - - E M G D S H V G L Q AG R V V E I F G P E S S G K T T L A L H T V A E A Q K K G G V C A F I D A E H A L D P L Y A R K L G V N L D D L L I S Q P D A - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A D T L V R S G A I D V L V V D S V A A L V P R A E L E G - - - - - E M G D S Q P G M Q AG R I V E I Y G P E S S G K T T L A L H C V A E A Q K K G G I C A F V D A E H A L D P V Y A R K L G V N V D D L L I S Q P D H - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A D T L V R S G A I D V L I V D S V A A L V P R A E L E G - - - - - E M G D A L P G L Q AG R V V E I Y G P E S S G K T T L A L H T V A E A Q K K G G I C A F I D A E H A L D P V Y A R K L G V N I D E L L I S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I C D T L V R S G A V D V L V V D S V A A L V P K A E L E G - - - - - E M G D A L P G L Q AG R I V E I Y G P E S S G K T T L T L H C V A E Q Q K A G G V C A F V D A E H A L D P T Y A K K L G V D L D E L L I S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I T D T L V R S G A V N M V I V D S V A A L T P K S E L E G - - - - - D M G D S S V G V Q AG R I I E I Y G P E S S G K T T L T L H V V A E E Q K K G G V C A F V D A E H A L D P Q Y A K K L G V N L D E L L I S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I V D T L V R S G A V N L I V V D S V A A L T P K S E I E G - - - - - D M G D M Q M G S Q AG R I V E I Y G P E S S G K T T L T L H C I A E E Q K K G G V C A F V D A E H A L D P Y Y A K K L G V S L E D L L I S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I V D T L V R S G A V S L V V V D S V A A L T P K A E I E G - - - - - D M G D A T V G A Q AG R I V E I Y G P E S S G K T T L T L E V I A A A Q R E G K T C A F I D A E H A L D P I Y A K K L G V D I D N L L C S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I C D A L T R S G A V D V I I V D S V A A L T P K A E I E G - - - - - E I G D S H M G L A AG R I V E I Y G P E S S G K T T L T L Q V I A A A Q R E G K T C A F I D A E H A L D P I Y A K K L G V D I D N L L C S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I C D A L T R S G A V D V I I V D S V A A L T P K A E I E G - - - - - E I G D S H M G L A AG R I V E I Y G P E S S G K T T L T L Q V I A A A Q R E G K T C A F I D A E H A L D P I Y A R K L G V D I D N L L C S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I C D A L A R S G A V D V I V V D S V A A L T P K A E I E G - - - - - E I G D S H M G L A AG R I V E I Y G P E S S G K T T L T L Q V I A A A Q R E G K T C A F I D A E H A L D P I Y A R K L G V D I D N L L C S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I C D A L A R S G A V D V I V V D S V A A L T P K A E I E G - - - - - E I G D S H M G L A AG R V V E I Y G P E S S G K T T L T L H I I A E A Q K A G G A A A F V D A E H A L D P D Y A E A V G V D I N D L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A D M L V R S G G L D V V V V D S V A A L T P K A E I E G - - - - - E M G D S H V G L Q AG R V V E I Y G P E S S G K T T L C L Q V V A E M Q K L G G V A A F I D A E H A L D P Q Y A Q K L G V N V G D L L I S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A D M L V R S G S V D I I V V D S V A A L T P R A E I E G - - - - - E M G D Q M V G L H AG R V I E V Y G P E S S G K T T L T L Q V I A E M Q K L G G T C A F V D A E H A L D V Q Y A S K L G V N L T D L L I S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I T D A L V R S G S V D L I V I D S V A A L V P K A E I E G - - - - - E M G D S L P G L Q AG R V I E V Y G P E S S G K T T L T L Q V I A E M Q K L G G T C A F V D A E H A L D V Q Y A S K L G V N L T D L L I S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I T D A L V R S G S V D L I V I D S V A A L V P K A E I E G - - - - - E M G D S L P G L Q AG R I V E I F G P E S S G K T T L C L H I V A E A Q K R G G I C G Y I D A E H A L D V G Y A R K L G V R T D D L L L S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E M L V R S G A I D V L V V D S V A A L V P K A E L E G - - - - - E M G D A H M G V Q AG R I I E I Y G P E S S G K T T L A L H A V A E A Q K K G G I A A F V D A E H A L D V G Y A R K L G V R T D D L L I S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E T L V R S G A I D V L V V D S V A A L V P K A E L E G - - - - - E M G D A H M G V Q AG R I I E I Y G P E S S G K T T L A L H A I A E A Q R R G G I A A F V D A E H A L D V G Y A R K L G V R T D D L L I S Q P D S - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I V E T L V R S G A I D V L V V D S V A A L V P K A E L E G - - - - - E M G D A H M G V Q AG R V I E V Y G P E S S G K T T L A L H I V A E A Q K S G G I A A F V D A E H A L D I N Y A R K L G V K T D D L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E T L V R S G A I D V L V I D S V A A L V P K A E I E G - - - - - E M G D S H V G L Q AG R I I E I Y G P E S S G K T T L A L H I A A E A Q K M G G I A A F V D A E H A L D I G Y A R K L G V K T D D L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E M L V R S G A V D V L V I D S V A A L V P K A E I E G - - - - - E M G D S H M G L Q AG R V I E I Y G P E S S G K T T L A L H I A A E A Q K L G G I A A F V D A E H A L D I G Y A R K L G V R T D D L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E M L V R S G A V D V L V I D S V A A L V P K A E I E G - - - - - E M G D S H V G L Q AG R I I E I Y G P E S S G K T T L T L H I I A E C Q K A G G V C A F I D A E H A L D V K Y A K N L G V N T D D L Y V S Q P D F - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I V E T I A R S G A V D L I V V D S V A A L T P K A E I E G - - - - - D M G D Q H V G L Q AG R V V E I Y G P E S S G K T T L A L Q I T A E C Q K A G G V C A F I D A E H A L D V G Y A K N L G V D V D N L L V S Q P D Y - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L D I V E T I A R S G A I D L I V I D S V A A L T P K S E I E G - - - - - E M S D Q N V G V Q AG R I I E I Y G P E S S G K T T L A L Q V I A E C Q K K G G I C A F I D A E H A L D V S Y A K R L G V D A E N L L V S Q P D Y - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I L E T L T R S G A V D L I V V D S V A A L T P K S E I D G - - - - - D M G D Q H V G L Q AG R I I E I Y G P E S S G K T T L S L H I I A E C Q K N G G V C A F I D A E H A L D V H Y A K R L G V D T E N L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I L E T I T R S G G I D L V V V D S V A A L T P K A E I D G - - - - - D M G D Q H V G L Q AG R I V E I Y G P E S S G K T T L A L H A I A E A Q K A G G I A A F V D A E H A F D Q S Y A R K L G I D I K S L L I S Q P E S - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L S I T E T L V R S G A V D I I V V D S V A A L V P Q A E L E G - - - - - E M G D S Q M G L Q AG R V T E I Y G P E S S G K T T L A L H V I A E A Q K E G G I A A L V D A E H A F D P S Y A R R L G V D I N S M L I S Q P E S - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L S I V E T L V R S G A V D V V V V D S V A A L V P Q A E L E G - - - - - E M G D S V V G L Q AG R V T E I Y G P E S S G K T T L A L H V I A E A Q K A G G I A A L V D A E H A F D P S Y A R K L G V D I N A M L I S Q P E S - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L S I V E T L V R S G A V D V V V V D S V A A L V P Q A E L E G - - - - - E M G D S V V G L Q AG R V T E I Y G P E S S G K T T L A L H V I A E A Q K A G G I A A I V D A E H A F D P S Y A R R L G V D I N A L L I S Q P E S - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L S I V E T L V R S G A V D V V A V D S V A A L V P Q A E L E G - - - - - E M G D T V V G L Q AG R V T E I Y G P E S S G K T T L A L H V I A E A Q K E G G I T A I V D A E H A F D P S Y A R K L G V D I N A L L I S Q P E S - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L S I V E T L V R S G A V D V V V V D S V A A L V P Q A E L E G - - - - - E M G D S S M G L Q AG R V T E I Y G P E S S G K T T L A L H V I A E A Q K E G G I A A I V D A E H A F D P T Y A R K L G V D I N A L L I S Q P E S - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L S I V E T L V R S N A I D V V V V D S V A A L V P Q A E L E G - - - - - E M G D S V M G L Q AG R V T E I Y G P E S S G K T T L A L H V I A E A Q K E G G I A A I V D A E H A F D P T Y A R K L G V D I N A L L I S Q P E S - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L S I V E T L V R S N A I D V V V V D S V A A L V P Q A E L E G - - - - - E M G D S V M G L Q AG R V T E I Y G P E S S G K T T L A L H V I A E A Q K E G G I A A I V D A E H A F D P T Y A R K L G V D I N A L L I S Q P E S - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L T I V E T L V R S G A V D V V V V D S V A A L V P Q A E L E G - - - - - E M G D S V M G L Q AG R V T E I Y G P E S S G K T T L A L H V I A E A Q K E G G I A A I V D A E H A F D P T Y A R K L G V D I N A L L I S Q P E S - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L T I V E T L V R S G A V D V V V V D S V A A L V P Q A E L E G - - - - - E M G D S V M G L Q AG R V T E I Y G P E S S G K T T L A L H A I A E A Q K G G G I A A M V D A E H A F D Q T Y A K K L G L D I G S L L V S Q P E S - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L S I V E T L V R S G A V D I V V V D S V A A L V P Q A E L E G - - - - - E M G D S V M G L Q AG R V T E I Y G P E S S G K T T L A L H A I A E A Q K N G G I A A L V D A E H A F D P T Y A R K L G V D I N A L L V S Q P E S - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L S I V E T L V R S G A V D I I V I D S V A A L V P Q A E L E G - - - - - E M G D S V V G L Q AG R V T E I Y G P E S S G K T T L A L H A I A E A Q K E G G I A A L V D A E H A F D P T Y A R K L G V D I N S L L V S Q P E S - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L S I V E T L V R S G A V D I V V V D S V A A L V P Q A E L E G - - - - - E M G D S V V G L Q AG R I V E I Y G P E S S G K T T L S M H C I A E A Q K A G G I A A F I D A E H A F D K T Y A E K L G I D T T N L L I S Q P D N - 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- - - - D M G D N K V G L Q AG R I I E I Y G N E S S G K T T L A L H S L A E V Q K L N G I V A F V D A E H A L D V E Y A R K L G V D V D K L I V S Q P D Y - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I V D S L V R S N I V D L I V V D S V A A L V P K A E I E G - - - - - A M G D S H M G L Q AG R I V E I F G Q E S S G K T T L A L H A I A E A Q K M G G V A A F I D A E H A L D P V Y A K N L G V D L K S L L I S Q P D H - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I V D E L V R S G V V D L I V V D S V A A L V P R A E I E G - - - - - A M G D M Q V G L Q AG R I V E I Y G P E S S G K T T I A L H A I A S A Q K N G G I A A F V D A E H A L D P V Y A K N L G V D V E N L L I A Q P D Y - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I V D E L V R S N A V D L V V I D S V A A L V P R A E I E G - - - - - S M G D V Q V G L Q AG R I I E I Y G P E S S G K T T I A L H A I A S V Q K L G G I A A F V D A E H A L D L N Y A K N L G I D L S E L L V S Q P D Y - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L D I V D E L V R S N A V D L I V V D S V A A L V P R A E I E G - - - - - A M G D A Q I G L Q AG R V I E I Y G P E S S G K T T L A L H A I A E I Q K T G G V A A F V D A E H A L D P A Y A K V L G V N V D D L I I S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - M A M E I V D Q L V R S A A I D V I V I D S V A A L V P R A E I E G - - - - - E M G D A H V G L Q AG R I I E V F G P E S S G K T S I A L H A I A E V Q K Q G G T A A F V D A E H A L D P T Y A A A L G V D I Q N L L I S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - A A L E I V D Q L V R S A A V D I V V V D S V A A L V P R A E I E G - - - - - D M G D A H V G L Q AG R I I E V F G P E S S G K T S V A L H A I A E V Q K R G G T A A F V D A E H A L D P A Y A A A L G V D I Q N L L I S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - A A L E I V D Q L V R S A A V D I V V V D S V A A L V P R A E I E G - - - - - D M G D A H V G L Q AG R I V E I Y G P E S S G K T T L A L H A V A A T Q Q A G G V A A F V D A E H A L D P V Y S K A L G V D I D N L L V A Q P D N - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - S A L E I V D Q L V R S T A V D I I V V D S V A A L V P R A E I E G - - - - - E M G D T S V G S Q AG R I V E I Y G P E S S G K T T V A L H A I A E V Q K A G G V A A F I D A E H A L D P T Y S A A L G V D I E N L L V A Q P D N - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - S A L E I A D Q L V R S A A V D L I V I D S V A A L V P R A E I E G - - - - - E M G D V Q V G L Q AG R I I E V Y G P E S S G K T T L T L H A I A E V Q K Q G G I A A F V D A E H A L D P V Y A T S V G V D I D N L L I S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - M A L E I V D Q L V R S A A V D I V V I D S V A A L V P R A E I E G - - - - - E M G D A Q V G L Q AG R V I E I Y G P E S S G K T T V A L H A I A E V Q K E G G I A A F V D A E H A L D P T Y A S A L G V D I Q N L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - S A L E I V D Q L V R S A A V D I V V I D S V A A L V P R A E I E G - - - - - D M G D A H V G L Q AG R V I E I Y G P E S S G K T T V A L H A L A E V Q R N G G I A A F V D A E H A L D P T Y A A A L G V D I D N L L I S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - S A L E I V D Q L V R S A A V D I V V I D S V A A L V P R A E I E G - - - - - D M G D A H V G L Q AG G V T L I G G E P G I G K S T L L L Q V A D K V A S R G G T V L Y V A G E E S L E Q I R L R A D R L G V A A D L Q M T R D T R A E H I A A L L E E H K P A L C I V D S I Q T V T V E G E G A P G G V A Q V R D G T A M L T R A A K E T G T A T V L V G H V T K D G T V A G P K V M E H I V D T T V F L E T

Cab. thermophilum RecASolibacter usitatus RecAAcidobacteria sp. Ellin345 RecADehalococcoides ethenogenes RecAHerpetosiphon aurantiacus RecAChloroflexus aggregans RecACfx. aurantiacus J-10-fl RecARoseiflexus sp. RS1 RecARoseiflexus castenholzii RecAThe. tengcongensis RecAThe. ethanolicus RecAClostridium novyi RecAClostridium botulinum RecAClostridium acetobutylicum RecAPel. thermopropionicum RecACar. hydrogenoformans RecAHb. modesticaldum RecADesulfitobacterium hafniense RecAStreptococcus pyogenes RecAStaphylococcus aureus RecAListeria monocytogenes RecABacillus subtilis RecABacillus cereus RecABacillus anthracis RecAAquifex pyrophilus RecAAquifex aeolicus RecADeinococcus radiodurans RecAThermus thermophilus RecAThermus aquaticus RecACorynebacterium glutamicum RecABrevibacterium linens RecAArthrobacter aurescens RecAStreptomyces coelicolor RecANocardia farcinica RecAErythrobacter litoralis RecABlastochloris viridis RecARhodopseudomonas palustris RecABradyrhizobium japonicum RecARoseobacter denitrificans RecARhodobacter sphaeroides RecARhodobacter capsulatus RecAShewanella oneidensis RecAProteus vulgaris RecAShigella flexneri RecAEscherichia coli RecAHalorhodospira halophila RecADechloromonas aromatica RecABordetella pertussis RecABordetella parapertussis RecAStigmatella aurantiaca RecAAnaeromyxobacter sp. RecAAnaeromyxobacter dehalogens RecAGeobacter lovleyi RecAGeobacter sulfurreducens RecAGeobacter metallireducens RecACampylobacter jejuni RecAThiomicrospira denitrificans RecAWolinella succinogenes RecAHelicobacter pylori RecAChloroherpeton thalassium RecAChlorobium ferooxidans RecAPelodictyon luteolum RecAChlorobium vibrioforme RecAChlorobium chlorochromatii RecAPld. phaeoclathratiforme RecAProsthecochloris aesturarii RecAChlorobium phaeobacteroides RecAChlorobium limicola RecAChlorobium phaeobacteroides BS1Chlorobium tepidum RecAChlorobaculum parvum RecACytophaga hutchinsonii RecAPorphyromonas gingivalis RecABacteriodes thetaiotaomicron RecABacteriodes fragilis RecAPetrotoga mobilis RecAThermotoga maritima RecAThermosipho melanesiensis RecAFervidobacterium nodosum RecAGloeobacter violaceus RecASynechococcus sp. JA-2-3B'a(2-13)Synechococcus sp. JA-3-3Ab RecASynechocystis sp. PCC 6803 RecASynechococcus sp. PCC 7002 RecASynechococcus sp. PCC 6301 RecANostoc sp. PCC 7120 RecANostoc punctiforme PCC 73102 RecADeinococcus radiodurans DR1105

171 305 170 303 172 305 169 302 169 302 109 242 171 304 171 304 171 304 168 301 167 300 168 301 172 305 168 301 168 301 168 301 167 300 170 303 182 319 168 301 167 300 166 280 167 300 167 300 174 307 171 304 181 315 168 302 168 302 181 314 179 312 170 303 169 302 171 304 179 312 167 300 181 314 180 313 181 314 181 314 181 314 170 303 170 303 170 303 170 303 170 303 169 302 177 310 177 310 172 305 172 305 172 305 168 301 169 302 169 302 168 302 168 302 170 304 170 304 182 315 182 315 181 314 182 315 183 316 181 314 181 314 181 314 181 314 181 314 182 315 183 316 177 311 177 310 183 316 185 318 175 313 171 309 171 309 170 308 185 318 167 301 167 301 171 305 172 306 174 307 172 305 172 305 300 446

R L M S Q A L R K L T A V A N R T N T C L I F I N Q I R E K I G - - - - - - - V T W G S P E T T T G G R A L K F Y A S V R I D I R R T A S I K D G - E E - - - V I G S R T K A K I A K N K L A P P F K E V E F D I I Y G K G I C R E G D L L D L G V A H K L I E K S G T W F S L K S - - - - G E R L G Q G RR L M S Q A L R K L T G S V N K S R T C L I F I N Q I R E K I G - - - - - - - V M F G N P E T T T G G R A L K F Y S T V R L D I R R I G A I K D G - D A - - - V V G N R T K V K V V K N K V A S P F R E A E F D I I Y G E G V S K E G D L L D L G V A Q N L V E K S G S W F S Y K - - - - - G D R I G Q G RR L M S Q A L R K L T G T V S K S G T C L I F I N Q I R E K I G - - - - - - - V M F G N P E T T T G G R A L K F Y S S V R V D I R R I A A I K D G - D T - - - V I G S R T K V K V V K N K T A A P F R E A E F D I V Y G E G I S K E G D M L D L A V E A N I V E K S G S W F S Y K - - - - - T E R I G Q G RR L M S Q A L R K L T A S I G Q T R T A V I F I N Q L R E K V G - - - - - - - V M F G N P E V T P G G R A L K F Y S S V R I D L R R I E T I K Q G - T V - - - A I G T R V R A K V V K N K V A P P F R T A E F D I M F D S G I S R E G N L I D L G V T S E V I R K A G A F F S Y G - - - - - D I R L G Q G RR L M S Q A L R K L S G A V S K S R T A L I F I N Q L R M K I G - - - - - - - V M F G N P E T T T G G N A L K F Y A S V R L D I R K V E S I K Q G - Q D - - - V T G A R A R V K V V K N K V A P P F R Q A E F D I M Y N E G I S R E G N I V D V G V T M E I L R K S G A W F Y L G - - - - - D D R L G Q G RR L M S Q A L R K L S G A I S K S R A V V I F I N Q L R M K I G - - - - - - - V M F G S P E T T T G G Q A L K F Y A S V R L D I R R I E T L K Q G - Q E - - - A I G S R V R V K V I K N K V A P P F R Q A E F D I L A N E G I S R E G N I I D I G T E L G I I R K S G A W F Y L G - - - - - E D R L G Q G RR L M S Q A L R K L S G A I S K S R T V V I F I N Q L R M K I G - - - - - - - V M L G T P E T T T G G Q A L K F Y T S V R L D I R R V E T L K Q G - Q E - - - A I G S R V R V K V I K N K V A P P Y R Q A E F D I L A N E G I S R E G N I I D I G T E L G I I R K S G A W F Y L G - - - - - E D R L G Q G RR L M S Q A L R K L S G A I S K S R A V V I F L N Q L R L K I G - - - - - - - V M F G S P E T T T G G Q A L K F Y A S V R M D I R R I E T L K N G - Q E - - - T I G S R T R V K V V K N K V A P P F R Q A E F D I M H N E G I S R A G N I L D V G V E L D I I R K S G A W F Y L G - - - - - D D R L G Q G RR L M S Q A L R K L S G A I S K S R V V V I F L N Q L R L K I G - - - - - - - V M F G S P E T T T G G Q A L K F Y A S V R M D I R R I E T L K N G - Q E - - - T I G S R T R V K V V K N K V A P P F R Q A E F D I M H N E G I S R A G N I L D V G V E L D I I R K S G A W F Y L G - - - - - E D R L G Q G RR L M S Q A L R K L A G V T S K S K S I V V F I N Q L R E K V G - - - - - - - V M F G N P E T T P G G R A L K F Y A T I R L D V R K V D N I K Q G - N E - - - I V G S R T R V K V V K N K I A P P F K Q A E F D I M Y G E G I S R E G S I L D L G T A L D I I E K S G S W Y S Y K - - - - - D I K L G Q E ER L M S Q A L R K L A G V T S K S K T I V V F I N Q L R E K V G - - - - - - - V L F G N P E T T P G G R A L K F Y A T I R L D V R K V D A I K Q G - N E - - - I I G N R T R V K V V K N K I A P P F K Q A E F D I M Y G E G I S R E G S I L D I G T S I D I I E K S G A W Y S Y G - - - - - D I R L G Q G RR L M S Q A L R K L T G S I N K S K C A T I F I N Q L R E K V G - - - - - - - I M F G N P E T T P G G R A L K F Y S S V R L D V R K I E T I K Q G - D E - - - F L G S R T R V K V V K N K V A P P F K Q A E F D I M Y G T G I S F E G N V L D V G V D N E I I Q K S G S W F S Y N - - - - - D T R L G Q G RR L M S Q A L R K L A G S I N K S K C V A I F I N Q L R E K V G - - - - - - - I M F G N P E T T P G G R A L K F Y S S V R L D V R R I D S I K Q G - D Q - - - I L G N R T R V K I N K N K V A P P F K M A E F D I M Y N E G I S K V G N I L D V G V R E E L V D K S G S W F S Y K - - - - - D T R L G Q G RR L M S Q A L R K L T G S I N K S K C I A I F I N Q L R E K V G - - - - - - - I M F G N P E T T P G G R A L K F Y A S V R M D I R K I D T I K Q G - E E - - - M I G N R V R V K V V K N K V A P P F K Q A E F D I M Y N E G I S R E G N I V D V G V K E N I I Q K S G A W F S Y G - - - - - D I R L G Q G RR L M S Q A L R K L T G A I S K S R T T V I F I N Q I R E K V G - - - - - - - V V Y G S P E T T T G G R A L K F Y A S I R L E V R K Q E N I K Q G - T D - - - I I G S R T R V K V V K N K V A P P F K Q A D F D I M Y G T G I S K E G S I L D M A T E L N I V E K S G A W Y S F G - - - - - E E R L G Q G RR L M S Q A L R K L T G A I S K S K T V A I F I N Q M R E K V G - - - - - - - V M F G N P E T T P G G R A L K F Y A S I R M D V R K I D V I K Q G - N D - - - I I G S R T R V K I V K N K V A P P F K Q A D F D I M Y G E G I S K E G S L L D V A V E L K I V Q K V G S W Y S Y N - - - - - D E R L G Q G RR L M S Q A L R K L T G V V S K S K T C C L F I N Q I R E K V G - - - - - - - V M F G N P E T T P G G R A L K F Y S S V R L E V R K V D T L K Q G - S D - - - M I G S R T R V K V V K N K V A P P F K V A D F D I M Y G E G I S R E G S L V D L A V D M G I V E K S G A W Y S Y K - - - - - G D R L G Q G RR L M S Q A L R K L T G A I S K S N T C V I F I N Q I R E K V G - - - - - - - I M F G N P E T T T G G R A L K F Y A S V R L E V R R A D T L K Q G - Q D - - - M I G N R T K V K V V K N K V A P P F K Q A E F D I M Y G E G I S R E G S M V D V G A E L D I I N K S G A W Y S Y K - - - - - G E R L G Q G RR M M S Q A M R K L S A S I N K T K T I A I F I N Q L R E K V G V - - - - - - - M F G N P E T T P G G R A L K F Y A S V R L D V R G T T Q I K G T G D Q K D S S I G K E T K I K V V K N K V A P P F K V A E V E I M Y G E G I S R T G E L V K I A S D L D I I Q K A G A W F S Y N - - - - - G E K I G Q G SR L M S Q A L R K L S G A I S K S N T T A I F I N Q I R E K V G V - - - - - - - M F G N P E T T P G G R A L K F Y S S V R L E V R R A E Q L K Q G Q E - - - - I V G N R T K I K V V K N K V A P P F R V A E V D I M Y G Q G I S K E G E L I D L G V E N D I V D K S G A W Y S Y N - - - - - G E R M G Q G KR L M S Q A L R K L S G V I N K S K T I A I F I N Q I R E K V G V - - - - - - - M F G N P E I T P G G R A L K F Y S T V R L E V R R A E Q L K Q G T D - - - - V M G N K T K I K V V K N K V A P P F R I A E V D I M Y G E G I S R E G E L V D M A A E V D V I N K S G S W Y S Y K - - - - - E E R I G Q G RR L M S Q A L R K L S G A I N K S K T I A I F I N Q I R E K V G V - - - - - - - M F G N P E T T P G G R A L K F Y S S V R L E V R R A E Q L K Q G N D - - - - V M G N K T K I K V V K N K V A P P F R T A E V D I M Y G E G I S K E G E I I D L G T E L D IR L M S Q A L R K L S G A I N K S K T I A I F I N Q I R E K V G V - - - - - - - M F G N P E T T P G G R A L K F Y S T V R L E V R R A E Q L K Q G N D - - - - I V G N K T K V K V V K N K V A P P F R V A E V D I M Y G E G I S R E G E I L D M A S E L D I V Q K S G A W Y S Y N - - - - - E E R L G Q G RR L M S Q A L R K L S G A I N K S K T I A I F I N Q I R E K V G V - - - - - - - M F G N P E T T P G G R A L K F Y S T V R L E V R R A E Q L K Q G N D - - - - I V G N K T K V K V V K N K V A P P F R V A E V D I M Y G E G I S R E G E I L D M A S E L D I V Q K S G A W Y S Y N - - - - - E E R L G Q G RR L M S Q A L R K L K G A V H R S N T A L I F I N Q I R E K I G - - - - - - - V M F G N P E T T P G G R A L K F F S D M R L E V R R L G - D V K E G G E K K - - G - Y R V K V R V V K N K L A P P F Q E A E F D V I Y G E G I C R I C D I I D T A A N L G V I T K S G S W Y S Y G - - - - - E K R L G Q G RR L M S Q A L R K L K G A V H K S N T A L I F I N Q I R E K I G - - - - - - - V M F G N P E T T P G G R A L K F F S D M R L E V R R L G - D V K E G G E K K - - G - Y R V K V R V V K N K L A P P F Q E A E F D I I Y G E G I C K L C D L I E V A T N L G I F T K S G S W Y S Y G - - - - - D K R L G Q G KR L M S Q A L R K L T A I L S K T G T A A I F I N Q V R E K I G - - - - - - - V M Y G N P E T T T G G R A L K F Y A S V R L D V R K I G Q P T K V G N D A V - - A - N T V K I K T V K N K V A A P F K E V E L A L V Y G K G F D Q L S D L V G L A A D M D I I K K A G S F Y S Y G - - - - - D E R I G Q G KR L M S Q A L R K L T A V L A K S N T A A I F I N Q V R E K V G - - - - - - - V T Y G N P E T T P G G R A L K F Y A S V R L D V R K S G Q P I K V G N E A V - - A - V K V R V K V V K N K L A P P F R E A E L E I Y F G R G L D P V A D L V N V A V A A G V I E K A G S W F S Y G - - - - - E L R L G Q G KR L M S Q A L R K L T A V L A K S N T A A I F I N Q V R E K V G - - - - - - - V T Y G N P E T T P G G R A L K F Y A S V R L D V R K S G Q P I K V G N E A V - - G - V K V R V K V V K N K L A P P F R E A E L E I Y F G R G L D P V A D L V N V A V A A G V I E K A G S W F S Y G - - - - - E L R L G Q G KR L M S Q A L R K M T G A L Y N S G T T A I F I N Q L R E K I G - - - - - - - V M F G S P E T T T G G K A L K F Y A S V R C D I R R I Q T L K D G - Q D - - - A I G N R T R L K V V K N K V S P P F K I A E F D I M Y G E G I S R E S S V I D L A V D N G I V K K S G S W F T Y E - - - - - G E Q L G Q G KR L M S Q A L R K I A G A L S Q S K T T A I F I N Q L R E K V G - - - - - - - V F F G S P E T T S G G K A L K F Y A S V R I D V R R I E T L K E G - Q D - - - A V G N R T R A K I V K N K I A P P F K Q A E F D I I Y G Q G I S R E G S L I D M G V D N G I I R K S G S W F T Y D - - - - - G D Q L G Q G KR L M S Q A L R K I T G R L S Q T K T T A I F I N Q L R E K I G - - - - - - - V F F G S P E T T T G G K A L K F Y A S V R I D V R R I Q T L K E G - A D - - - S V G N R T K A K I V K N K M A P P F K I A E F D I I Y G Q G I S R E G G I I D M G V E H G I I K K S G S W F T Y D - - - - - G D Q L G Q G MR L M S Q A L R K I T S A L N Q S K T T A I F I N Q L R E K I G - - - - - - - V M F G S P E T T T G G R A L K F Y A S V R L D I R R I E T L K D G - T D - - - A V G N R T R V K V V K N K V A P P F K Q A E F D I L Y G Q G I S R E G G L I D M G V E N G F V R K A G A W Y T Y E - - - - - G D Q L G Q G KR L M S Q A L R K M T S A L N N S G T T A I F I N Q L R E K I G - - - - - - - V M F G S P E T T T G G K A L K F Y A S V R L D V R R I E T L K D G - S D - - - A V G N R T R V K V V K N K V S P P F K Q A E F D I L Y G Q G I S K E G S L I D M G V E Q G F I R K S G S W Y T Y E - - - - - G D Q L G Q G KR L M S Q S L R K L T G S I N R S K C M V I F I N Q L R M K I G - - - - - - - V M Y G N P E T T T G G N A L K F Y A S V R L D I R R T G Q I K D R - D E - - - V V G N S T R V K V V K N K V A P P F K Q V E F D I M Y G E G I S K I G E I L D L G V K A G I V E K S G S W F S Y D - - - - - S I R I G Q G RR L M S Q A L R K L T A S I S R S N C M V I F I N Q I R M K I G - - - - - - - V M Y G S P E T T T G G N A L K F Y A S V R L D I R R I G A I K E R - D E - - - V V G N Q T R V K V V K N K L A P P F K Q V E F D I M Y G E G I S K A G E L I D L G V K A G V V D K S G S W F S H D - - - - - S Q R I G Q G RR L M S Q A L R K L T A S I N K S N T M V I F I N Q I R M K I G - - - - - - - V M Y G S P E T T T G G N A L K F Y A S V R L D I R R I G Q I K E R - D E - - - V V G N Q T R V K V V K N K L A P P F K Q V E F D I M Y G E G V S K M G E I L D L G V K A G I V E K S G A W F S H D - - - - - S Q R L G Q G RR L M S Q A L R K L T A S I N K S H T M V I F I N Q I R M K I G - - - - - - - V M Y G S P E T T T G G N A L K F Y A S V R L D I R R I G A I K E R - D E - - - V V G N T T R V K V V K N K L A P P F K Q V E F D I M Y G E G V S K M G E I L D L G V K A G I V E K S G A W F S Y D - - - - - S Q R L G Q G RR L M S Q A M R K L T G S I S R S N C M V I F I N Q I R M K I G - - - - - - - V M F G S P E T T T G G N A L K F Y S S V R L D I R R I G A L K D R - D E - - - V V G N H T R V K V V K N K V A A P F K Q V E F D I M Y G E G I S K M G E L L D L G V A A G V V D K S G S W F S Y G - - - - - D E R I G Q G RR L M S Q A M R K L T A S I G R S N C M V I F I N Q I R M K I G - - - - - - - V M F G N P E T T T G G N A L K F Y A S V R L D I R R T G A I K D R - D N - - - V I G N T T K V K V V K N K V A P P F R E V E F D I M Y G E G I S K T G E L V D L G V K A G V V E K S G S W Y S Y G - - - - - D E R I G Q G RR L M S Q A M R K L T A S I G R S N C M V I F I N Q I R M K I G - - - - - - - V M F G S P E T T S G G N A L K F Y A S V R L D I R R T G A I K D R - D E - - - V I G N Q T R V K V V K N K V A P P F R E V E F D I L Y G E G I S K V G E L V D L G V K A G V V A K S G A W Y S Y G - - - - - D E R I G Q G RR M M S Q A M R K L A G N L K Q S N T L L I F I N Q I R M K I G - - - - - - - V M F G N P E T T T G G N A L K F Y A S V R L D I R R T G A I K E G - D E - - - V V G N E T R V K V V K N K V A A P F K Q A E F Q I L Y G Q G I N R T G E L V D L G V A H K L I E K A G A W Y S Y K - - - - - G D K I G Q G RR M M S Q A M R K L A G N L K Q A N T L L I F I N Q I R M K I G - - - - - - - V M F G N P E T T T G G N A L K F Y A S V R L D I R R T G A I K D G - E E - - - V V G S E T R V K V V K N K V A A P F K Q A E F Q I L Y G E G I N I H G E L V D L G V K H K L I E K A G A W Y S Y N - - - - - G D K I G Q G KR M M S Q A M R K L A G N L K Q S N T L L I F I N Q I R M K I G - - - - - - - V M F G N P E T T T G G N A L K F Y A S V R L D I R R I G A V K E G - E N - - - V V G S E T R V K V V K N K I A A P F K Q A E F Q I L Y G E G I N F Y G E L V D L G V K E K L I E K A G A W Y S Y K - - - - - G E K I G Q G KR M M S Q A M R K L A G N L K Q S N T L L I F I N Q I R M K I G - - - - - - - V M F G N P E T T T G G N A L K F Y A S V R L D I R R I G A V K E G - E N - - - V V G S E T R V K V V K N K I A A P F K Q A E F Q I L Y G E G I N F Y G E L V D L G V K E K L I E K A G A W Y S Y K - - - - - G E K I G Q G KR L M S Q A L R K L T G S I Q R S N T L V V F I N Q I R M K I G - - - - - - - V M F G S P E T T T G G N A L K F Y S S V R M D I R R L G A I K K G - D E - - - V L G N E T R V K V V K N K M A P P F K Q A E F E I L Y G E G I S R E G E I I D M G V A Q G F V D K S G A W Y S Y N - - - - - G D R I G Q G KR L M S Q A L R K L T A N I K K T N T L V I F I N Q I R M K I G - - - - - - - V M F G S P E T T T G G N A L K F Y A S V R L D I R R I G A I K K G - D E - - - V I G S E T K V K V V K N K V A P P F R E A I F D I L Y G E G I S R Q G E I V E M G V A H K L V D K S G A W Y A Y K - - - - - G E K I G Q G KR L M S Q A L R K L T A T I K R T N C M V I F I N Q I R M K I G - - - - - - - V M F G N P E T T T G G N A L K F Y S S V R L D I R R I G A I K K G - D E - - - V V G N E T R V K V V K N K V A P P F K Q A E F D I M Y G S G I S R E G E I I D L G V Q A N V V D K S G A W Y S Y S - - - - - G N R I G Q G KR L M S Q A L R K L T A T I K R T N C M V I F I N Q I R M K I G - - - - - - - V M F G N P E T T T G G N A L K F Y S S V R L D I R R I G A I K K G - D E - - - V V G N E T R V K V V K N K V A P P F K Q A E F D I M Y G S G I S R E G E I I D L G V Q A N V V D K S G A W Y S Y S - - - - - G N R I G Q G KR L M S Q A L R K L T G T I S K S Q T C V I F I N Q I R M K I G - - - - - - - V M F G N P E T T T G G N A L K F Y A S Q R L D I R R I G A I K N G - E N - - - V V G S R T R V K V V K N K V A P P F K E V E F D I M Y G T G I S R E G D L I D L A S N E N I V E K S G S W F A F K - - - - - G E R I G Q G RR L M S Q A L R K L T G T I S K S S T I V I F I N Q I R M K I G - - - - - - - V M F G N P E T T T G G N A L K F Y A T Q R L D I R R I G A I K D G - D Q - - - V I G N R T R V K V V K N K V A P P F K E V E F D I M Y G H G I S R E G D L L D L G S N E N I V E K S G T W F S F G - - - - - G E R I G Q G RR L M S Q A L R K L T G T I S K S S T I V I F I N Q I R M K I G - - - - - - - V M F G N P E T T T G G N A L K F Y A S Q R L D I R R I G A I K D G - D S - - - V I G N R T R V K V V K N K V A P P F K E V E F D I M Y G H G I S R E G D V L D L A S N E G I V E K S G T W F S F G - - - - - G E R I G Q G RR L M S Q A L R K L T G I I S K S N C C V I F I N Q I R M K I G - - - - - - - V M F G N P E T T T G G N A L K F Y A S V R L D I R K I A T L K Q G - D Q - - - V I G S R T R V K V V K N K V A P P F K E A E F D I L Y G E G I S R T G D V L D L A V D R N I I D K S G A W F S Y N - - - - - K E R I G Q G RR L M S Q A L R K L T G I I S K S N C C V I F I N Q I R M K I G - - - - - - - V M F G N P E T T T G G N A L K F Y A S V R L D I R K I A S L K Q G - Q D - - - V I G S R T K V K V V K N K V A P P F K E V E F D I Y Y G E G I S R E G D I L D L A V E K G I V D K S G A W F S Y G - - - - - G D R I G Q G RR L M S Q A L R K L T G I I S K S N C C V I F I N Q I R M K I G - - - - - - - V M F G S P E T T T G G N A L K F Y A S V R L D I R K I A T L K Q G - D A - - - V I G S R T K V K V V K N K V A P P F R E V E F D I Y Y G E G I S R L G D L L D L A V D R K I I D K S G A W F S Y G - - - - - S D R I G Q G RR L M S Q A L R K L T G I V H K M N T T V I F I N Q I R M K I G - - - - - - A M G Y G T P E T T T G G N A L K F Y A S V R L D V R K V A T L K Q N - E E - - - P I G N R V K V K V V K N K V A P P F R Q A E F D V M F G E G L S R E G E L I D Y G V K L D I V D K S G A W F S Y K - - - - - D K K L G Q G RR L M S K A L R K L T G I L H K M N C T V I F I N Q I R M K I G - - - - - - M M G Y G S P E T T T G G N A L K F Y A S V R I D V R R I A S L K Q G - E S - - - Q I G N R V K A K V I K N K V A P P F R Q A E F D I M F G E G I S K E G E L V D Y G V K L D I I D K S G A W F S Y G - - - - - E T K L G Q G RR L M S Q A L R K V T G V L H K M N T T V I F I N Q I R M K I G - - - - - - M M G Y G S P E T T T G G N A L K F Y A S V R I D V R R I A S L K Q G - E Q - - - H I G N R V K A K V V K N K V A P P F R E A E F D I M F G E G I S Q E G E L I D Y G V K L D I V D K S G S W L S Y G - - - - - D K K L G Q G KR L M S H A L R K I T G V L H K M N T T L I F I N Q I R M K I G - - - - - - M M G Y G S P E T T T G G N A L K F Y A S V R I D I R R I A S L K Q N - E Q - - - H I G N R A K A K V V K N K V A P P F R E A E F D I M F G E G I S K E G E I I D Y G V K L D I V D K S G A W L S Y Q - - - - - D K K L G Q G RR L M S Q A L R K L T G A I S K S N C V A I F I N Q L R D K I G - - - - - - - V M Y G S P E T T T G G K A L K F Y A S V R L D I R K I A Q I K D G T E - - - - I V G N R T K V K V V K N K V A P P F K T V E F D I I Y G E G V S R I G E L I D L A V E L G I V K K A G A W F S Y E - - - - - N D K L G Q G RR L M S Q A L R K L T G A I S K S S C V C I F I N Q L R D K I G - - - - - - - V M Y G S P E T T T G G K A L K F Y S S I R L D I R R I A Q L K D G D E - - - - I I G N R T K V K V V K N K V A P P F R T A E F D I L Y G E G I S V I G E L I D L A V E F G V V K K S G S W F S Y G - - - - - P D K L G Q G RR L M S Q A L R K L T G A I S K S S S V C I F I N Q L R D K I G - - - - - - - V M Y G S P E T T T G G K A L K F Y S S I R L D I R K I A Q L K D G D E - - - - I I G N R T K V K V V K N K V A P P F R I A E F D I L Y G E G I S L M G E L I D L A V E F G V V K K A G S W F S Y G - - - - - P D K L G Q G RR L M S Q A L R K L T G A I S K S S C S C I F I N Q L R D K I G - - - - - - - V M Y G S P E T T T G G K A L K F Y S S I R L D I R R I A Q L K D G D A - - - - I I G N R T K V K V V K N K V A P P F R I A E F D I L Y G E G I S M L G E L I D L A V E F G V V K K A G S W F S Y G - - - - - P D K L G Q G RR L M S Q A L R K L T G A I S K S S T V C I F I N Q L R D K I G - - - - - - - V M Y G S P E T T T G G K A L K F Y S S V R L D I R K I A Q L K D G D E - - - - L T G S R T R V K V V K N K V A P P F K M A E F D I L Y G E G I S A L G E L I D L G V E F G V I K K A G S W F S Y G - - - - - T E K L G Q G RR L M S Q A L R K L T G A I S K S S C V C I F I N Q L R D K I G - - - - - - - V V Y G N P E T T T G G K A L K F Y S S V R L D I R K I A Q I K D G E E - - - - I T G N R T R V K V V K N K V A P P F R T A E F D I L Y G E G I S A M G E L I D L A V E F G V V K K A G S W F S Y G - - - - - T D K L G Q G RR L M S Q A L R K L T G A I S K S S C V C I F I N Q L R D K I G - - - - - - - V V Y G N P E T T T G G K A L K F Y S S V R L D I R K I A Q I K D G E E - - - - I T G N R T R V K V V K N K V A P P F R T A E F D I L Y G E G I S A M G E L I D L A V E F G V V K K A G S W F S Y G - - - - - T D K L G Q G RR L M S Q A L R K L T G A I S K S S T V C I F I N Q L R D K I G - - - - - - - V M Y G S P E T T T G G K A L K F Y A S V R L D I R K I A Q I K D G D E - - - - I V G S R T K V K V V K N K V A P P F K T A E F D I L Y G E G I S S M G E L I D L A V E F A V V K K A G S W F S F G - - - - - Q E K L G Q G RR L M S Q A L R K L T G A I S K S S T V C I F I N Q L R D K I G - - - - - - - V M Y G S P E T T T G G K A L K F Y A S V R L D I R K I A Q I K D G D E - - - - I V G S R T K V K V V K N K V A P P F K T A E F D I L Y G E G I S S M G E L I D L A V E F A V V K K A G S W F S F G - - - - - Q E K L G Q G RR L M S Q A L R K L T G A I S K S S A V V I F I N Q L R D K I G - - - - - - - V V Y G N P E T T T G G K A L K F Y S S V R L E I R K I A Q I K D G A E - - - - I I G N R T K V K V V K N K V A P P F K I V E F D I L Y G E G I S V L G E L I D L A V E F G I V K K A G A W F S Y D - - - - - S D K L G Q G RR L M S Q A L R K L T G A I S K S S S V C L F I N Q L R D K I G - - - - - - - V M Y G S P E T T T G G K A L K F Y S S V R L D I R K I A Q I K D G E E - - - - L V G N R T K V K V V K N K V A P P F K T A E F D I L Y G E G I S V L G E L I D L A V E F G I I K K S G A W F S Y G - - - - - T E K L G Q G RR L M S Q A L R K L T G A I S K S S S V C I F I N Q L R D K I G - - - - - - - V M Y G S P E T T T G G K A L K F Y S S V R L D I R R I A Q I K D G E E - - - - V V G N R T K V K V V K N K V A P P F K I A E F D I L Y G E G I S V L G E L I D L A V E F G I I K K A G A W F S Y G - - - - - S E K L G Q G RR L M S Q A L R K L T G A I N K T G C C C I F I N Q L R E K I G - - - - - - - V M F G N P E T T T G G N A L K F Y A S V R L D I R R I G Q I K E S A D N - - - I T G N R T K V K V V K N K M A P P F K V I E F D I M Y G E G I S K I G E I I D L G V E L G I I N K A G S W F S Y E - - - - - G T K L G Q G RR L M S Q A L R K M T G A I S K S N T T C I F I N Q L R E K I G - - - - - - - V L F G N P E T T T G G N A L K F Y A S I R I D I R K S T P I K D G E E - - - - I M G H L T K V K V L K N K V A P P F R K A E F D I V F G E G I S R S G E I I D L G V E L D I I K K S G S W F S Y G - - - - - D T K L G Q G RR L M S Q A L R K L T G T V S K T R T T C I F I N Q L R E K I G - - - - - - - V M F G N P E T T T G G N A L K F Y A S V R I D I R G S Q P I K D G E E - - - - V L G K L T K V K V V K N K V A P P F R K A E F D I M F G E G I S H S G E I I D L G A E L G I I K K S G S W Y S Y N - - - - - D T K L G Q G RR L M S Q A L R K L T S A V S K T R T T C I F I N Q L R E K I G - - - - - - - V M F G N P E T T T G G N A L K F Y A S V R L D I R G S Q Q I K D G E E - - - - V I G K Q T K V K V V K N K V A P P F R K A E F D I M F G E G I S H S G E I I D L G A D L G I I K K S G S W Y S Y N - - - - - D T K L G Q G RR L M S Q A L R K L A G S I N K S K S I V I F I N Q V R M K I G - - - - - - - V V Y G N P E T T T G G I A L K F Y A T I R V E V R K G N P I R E G K D Q - - - - I G N E T T L K V V K N K V A P P F K Q A N V D M I F G R G I P K E N D I F N I A V E E E L I Q R K G A W F S F I N E N G E E I S L G Q G KR L M S Q A L R K I A G S V N K S K A V V I F T N Q I R M K I G - - - - - - - V M F G S P E T T T G G L A L K F Y A T M R M E V R R G E P I K E G K D V - - - - I G N V I S V K I V K N K V A P P F K T A Q T Y I I Y G K G I D R E Y E L F N I A V N E G I V D R K G S W Y Y Y T T L K G E E V S L G Q G SR L M S Q A L R K L A G N I N K S K A V V I F I N Q T R M K I G - - - - - - - V M Y G N P E T T T G G V A L K F Y S T M R L E V R S I G K I T E G K E S - - - - I G N Q V R I K F V K N K V A P P F K E A I V D V I Y G K G I V R E N E L F N L A V D E G L I S R R G S W F S Y V D S T G T E H S L G Q G KR L M S Q A L R K L A G N V N K S K V V V I F I N Q T R M K I G - - - - - - - V M Y G N P E T T T G G V A L K F Y A T M R I E V R S A G K I V E G N E H - - - - I G N E V N I K F V K N K V A P P F R T A S V D I I Y G K G I V R E N E L F N I A V N E G L I D R R G S W F T Y E D L S G K E H S L G Q G KR L M S Q A L R K I T G N I G K T G C M V I F L N Q L R S K I G - - - - - - - V M Y G N P E T T T G G N A L K F Y A S V R L D I R K A E T L K K G - Q D - - - E Y G N R V R V K V V K N K V A P P F R K A E F D I I F G K G I S S L G C I L D L A V E M E I V E R K G A W Y S Y G - - - - - S E R L G Q G RR L M S Q A L R K I A G N V S K T G S I V L F L N Q L R S K I G - - - - - - G I G Y G P Q E T T T G G N A L K F Y A S V R L D I R R V Q T L K K G - A E - - - E Y G I R A R V K V V K N K V A P P F R R A E F D I I F G Q G I S N I G C I I D L A D E M G I L K R R G S W Y S Y K - - - - - E E N V A Q G RR L M S Q A L R K I A G N V S K T G S I V L F L N Q L R S K I G - - - - - - G I G Y G L Q E T T T G G N A L K F Y A S V R L D I R R V Q T L K K G - A E - - - E Y G I R A R V K V V K N K V A P P F R R A E F D I I F G Q G I S N I G C I V D L A E E M G I L K R R G S W Y S Y K - - - - - E E N V A Q G RR L M S K A M R K I A G N I G R S G C L V I F L N Q L R Q K I G - - - - - - - V T Y G S P E V T T G G N A L K F Y A S V R L D I R R I Q T L K K G T E G - - - E Y G I R A K V K V A K N K V A P P F R I A E F D I I F G Q G I S R M G C T I D L A E K C E V I T R K G A W Y S Y N - - - - - G E N I A Q G RR L M S K A L R K I A G N M G R S G C T V I F L N Q L R Q K I G - - - - - - - I S Y G N P E V T T G G T A L K F Y A S V R L D I R R I Q T L K K G S E G - - - E F G I R A K V K V A K N K V A P P F R I A E F D I I F G K G I S R V G C M L D L A E Q T G V I T R K G A W Y S Y E - - - - - G D N I A Q G RR L M S Q A M R K I T G N I G K S G C T V I F L N Q L R Q K I G - - - - - - - V T Y G S P E T T T G G Q A L K F Y A S V R L D I R R I Q T L K K G - T E - - - E Y G T R A K V K V V K N K V A P P F R I A E F D I L F G K G I S T L G C L V D L A E E T G V I L R K G A W Y S Y N - - - - - G D N I G Q G RR L M S Q A L R K I T G N I G K S G C T V I F I N Q L R Q K I G - - - - - - - V T Y G S P E T T T G G N A L K F Y A S V R L D I R R I Q T L K K G - T D - - - E F G N R V K V K V A K N K V A P P F R I A E F D I I F G K G V S T L G C L V D L A E E T G I L I R K G A W Y S Y N - - - - - G D N I S Q G RR L M S Q A L R K I T G N I G K S G C T V I F I N Q L R Q K I G - - - - - - - V T Y G S P E T T T G G N A L K F Y A S V R L D I R R I Q T L K K G - T D - - - E Y G N R V K V K V A K N K V A P P F R I A E F D I I F G K G I S T L G C V V D L A E E T G I I I R K G A W Y S Y N - - - - - G D N I S Q G RV G A F R L L R S V K N R F G Q A G E L G V F E M R G E G L I A V D N P S A A F L A E R P L D V P G S V V A A T V D G Q R P M L L E V Q A L A S K T P Y P N - A R R V V V G L D P R R V D V V L A V L E R R L D L T L G G L D V Y V N L A G G L K V P D P G L D L A V A L A V Y S - - A V V G R A L P Q N V

Cab. thermophilum RecASolibacter usitatus RecAAcidobacteria Ellin345 RecADehalococcoides ethenogenes RecAHerpetosiphon aurantiacus RecAChloroflexus aggregans RecACfx. aurantiacus J-10-fl RecARoseiflexus sp. RS1 RecARoseiflexus castenholzii RecAThe. tengcongensis RecAThe. ethanolicus RecAClostridium novyi RecAClostridium botulinum RecAClostridium acetobutylicum RecAPel. thermopropionicum RecACar. hydrogenoformans RecAHb. modesticaldum RecADesulfitobacterium hafniense RecAStreptococcus pyogenes RecAStaphylococcus aureus RecAListeria monocytogenes RecABacillus subtilis RecABacillus cereus RecABacillus anthracis RecAAquifex pyrophilus RecAAquifex aeolicus RecADeinococcus radiodurans RecAThermus thermophilus RecAThermus aquaticus RecACorynebacterium glutamicum RecABrevibacterium linens RecAArthrobacter aurescens RecAStreptomyces coelicolor RecANocardia farcinica RecAErythrobacter litoralis RecABlastochloris viridis RecARhodopseudomonas palustris RecABradyrhizobium japonicum RecARoseobacter denitrificans RecARhodobacter sphaeroides RecARhodobacter capsulatus RecAShewanella oneidensis RecAProteus vulgaris RecAShigella flexneri RecAEscherichia coli RecAHalorhodospira halophila RecADechloromonas aromatica RecABordetella pertussis RecABordetella parapertussis RecAStigmatella aurantiaca RecAAnaeromyxobacter sp. RecAAnaeromyxobacter dehalogens RecAGeobacter lovleyi RecAGeobacter sulfurreducens RecAGeobacter metallireducens RecACampylobacter jejuni RecAThiomicrospira denitrificans RecAWolinella succinogenes RecAHelicobacter pylori RecAChloroherpeton thalassium RecAChlorobium ferooxidans RecAPelodictyon luteolum RecAChlorobium vibrioforme RecAChlorobium chlorochromatii RecAPld. phaeoclathratiforme RecAProsthecochloris aesturarii RecAChlorobium phaeobacteroides RecAChlorobium limicola RecAChlorobium phaeobacteroides BS1Chlorobium tepidum RecAChlorobaculum parvum RecACytophaga hutchinsonii RecAPorphyromonas gingivalis RecABacteriodes thetaiotaomicron RecABacteriodes fragilis RecAPetrotoga mobilis RecAThermotoga maritima RecAThermosipho melanesiensis RecAFervidobacterium nodosum RecAGloeobacter violaceus RecASynechococcus sp. JA-2-3B'a(2-13)Synechococcus sp. JA-3-3Ab RecASynechocystis sp. PCC 6803 RecASynechococcus sp. PCC 7002 RecASynechococcus sp. PCC 6301 RecANostoc sp. PCC 7120 RecANostoc punctiforme PCC 73102 RecADeinococcus radiodurans DR1105

306 351 304 355 306 361 303 337 303 358 243 289 305 351 305 353 305 353 302 307 301 341 302 350 306 352 302 349 302 342 302 343 301 322 304 346 320 378 302 347 301 348 281 280 301 343 301 343 308 348 305 345 316 363 303 340 303 340 315 376 313 370 304 347 303 374 305 347 313 352 301 348 315 363 314 362 315 359 315 357 315 355 304 357 304 325 304 353 304 353 304 354 303 343 311 353 311 353 306 360 306 358 306 358 302 336 303 338 303 339 303 343 303 345 305 350 305 347 316 357 316 353 315 348 316 349 317 353 315 348 315 348 315 350 315 350 315 350 316 346 317 347 312 355 311 340 317 344 319 347 314 368 310 356 310 349 309 357 319 369 302 358 302 358 306 354 307 348 308 356 306 357 306 359 447 503

E N A K Q A L A A N P A L R E Q L E R E L R A I L M P H R V P A T P P E G N A K A K G A S EE N A R Q F L K D N P D I R K A V D T E L R K T L G L V K S D A A P V P P T P A P E P A A A K P A A R RE N A K Q F L K D N K D I A A R V E A E V R K H V G L V K E P A A P A P N A A P P V P P N G H A E K A A V A R KE S A K N Y L A A N P D L A Q E I E E K I R A S A V T L C S I G D G DE N A K Q F L K D N P A L A D E I E K M I R A A S P G A P T A K V I V G A A A A N G A A P A D D G D G I F D D EE N V R E F L K N N P A L T D E I E R L I K A Q A L T N P S A I A P S - - A D I G D D D G V F E EE N V R E F L K N N P A L T D E I E R L I K A Q A L T N P T V I A P S - - V D V G D D D A I F E EE N A K Q F L N E N P A L A D E I E R L I R A H A M A A P I S I V S P K E D D A V E D A G L F E EE N A K Q F L N E N P A L A D E I E R L I R A H A M A A P I S I A A S K A D D V V D D A G L F E EK M Q S S FE N A K Q F L K E N K E I A D E I E R K I R E N F N L A Y N K I K S S P D A I V EE N V K Q F L R E N P S I L L E V E N K I R E K H N L K T R N T A D S K V T G A K D E K S K E E KE N A K Q F L K D N M N I A L E I E N T I R K K H D L P V V D A K N L N I E N K K E D N K E GE N A K L F F K E N I D I R S E V E N K I R E K Y D L P I Q K V K N E K P K I E K G T E N N T KE N V K E Y L R E H P E I T R E I E R K V R Q A L N V G G F K A P A A T V S E E GE N A K E F L R Q N P E I Y R E I E Q K I R E Q V L N S P E S S S F K N S E E F E EE N V K E F L R N H P A L A A E I E S M I RE N V K E F L K K N P E I A A E I E Q G I R A S I A N V S A P V A D D V Q E E P E D IE N A K R Y L A D H P E L F D E I D R K V R V K F G L L E E S E E E S A M V V A S E E I D D L A L D L D N G I E I E DE N V K M Y L K E N P Q I K E E I D R K L R E K L G I S D G D V E E T E D A P K S L F D E EE N A K Q Y L K E H T D I R D E I S K R V R E E Y E I D G S S K E P L D E K E E T L S L L D D E

E N S K Q F L K E N T D L R E E I A F F I R E H H G I S E D S G A E D M E D P S L L DE N S K Q F L K E N T D L R E E I A F F I R E H H G I S E D S G A E G M E D P N L L DE Q A K K Y L L E H P E M L E E I E R K V R E V S G L V R P D T E N S V G E K S EE Q V K K Y L Q E H P E L I D E I D Q K V R E A A G L A G S N I E E S T G E E G KE K T I A Y I A E R P E M E Q E I R D R V M A A I R A G N A G E A P A L A P A P A A P E A A E AE K A A E A L R E R P E L L E E I R T K V L E R A G E V V L A A G E D E G EE K A A E A L R E R P E L L E E I R A K V L E R S D Q V V L A A G E D E G EE K V R L S L K E N P E L T D E L E D K I F K K L G V G K Y A A A S D E L T D D P V E L V P N V D F D D E A D T E A D A E DE N V R N F L R D N P G L A E E I E L K I K H K M G L I K V D E P E A D A D A N Q A D G A S A E S S K S D D V E V SE N S R R F L R D N P E L A Q E L E R L I K E K L G V G V V K T E E D S P K L K A V D GE N A R N F L K D N P D L A N E I E K K I K Q K L G V G V H P E E S A T E P G A D A A S A A P A D A A P A V P A P T T A K A T K S K A T A A K SE N A R K F L L E N T D V R D E I E K K I K E K L G I G A D L T A E D A A E V P A D FE N A K T Y L K E N P E I C D K L E A A I R G K T D E V A E E M M T G P D A E DE N V K G F L K G H P D V A G R I E A A I R Q N A G L I A E R I L G S S - E D S E D G E D A A E AE N A K A F L K A N P D M T A K I E A A I R Q N S G L I A E Q I L A G S P E S D A D G E E P M E EE N S K A F L K A N P D I T A K I E T A I R Q N S G L I A E Q I L A G T P E R D A D G E E P A D EE N A K G F L R E N V Q M A L E I E D K I R A A H G L D F D M P A H E K K A D D D I L E AE N A K A F L R A N P T V A G D I E D R I R A S H G L D F S T G E D G K G D D L V D ME N A K Q F L R D N P D I A Y E I E D K I R A S H G L E F G V D P T A E D L T E EA N A G K Y L T E N P A I A A E I D K T L R E L L L S N P S A L A S S A S D D D N V E G N I D L E T G E V FA N A C N F L K E N S L V K E T K N F N G CA N A T A W L K D N P E T A K E I E K K V R E L L L S N P N S T P D F S V D D S E G V A E T N E D FA N A T A W L K D N P E T A K E I E K K V R E L L L S N P N S T P D F S V D D S E G V A E T N E D FD N A R Q F L R N N P Q I A A D I E A R I R E K V L G T A L P E A E P T E D A A S D T E Q K S G K G SD N A R E F L K A N P E I A Q E I E A G I R E A A S T N V I K P V K A A K A A D AD N V R E Y L K E H K E M A I E I E N K V R E N Q G I V S R A A T F P A S E A E D G ED N V R E Y L K E H K E M A I E I E N K V R E N Q G I V S R A A T F P A S E A E D G EE N A K D Y L R E H P D T Y K E I E A R V L E K Y G V T K P A V A A V P D E P E P S S E G E K R P R V K A V KE Q A K A F L R E H P E V L Q Q V E G K L F E K F G I R R G P V A V P P P P P D E A E E K K G R A R S G RE Q T K A F F R E H P E I L Q Q V E A R L F E K F G I H R G P V A V P S P P A E E P A E E R K P R A K A KE N S R Q F L K E N P V M L A E I E A R L M E L I K P A P K E E K A EE N S R L F L K E R P E L V N E I E G K V Y D V A G I P R K G A K E A AE N S R N F L K E H P E M V A E I E D K I Y E T A G I P R K G G K E E A AE N S K A F L K E N P E I A D E I T K A I Q N S M G I E G M I S G S E D D E G E EE N V K A K F M E D K V L A H E I E E K I K A A M G L G D L M T M D S L D I E D V D LE N A K A F L K E N K E I A N E I Q A K I I G A I G S S D E I T T F G D D E D E S E N L E SE N A K A L L K E D K A L A D E I T L K I K E S I G S N E E I M P L P D E P L E E M EE T V K T L L K A D E A L F N K I Y A Q V K E Q M V G L K L E G S S D D D S S S D SE T V K K A L R E D P V L F K K I H A Q V K E L M K V P A E I V K D K E Q AE A V K K A L R E D P E L F K K V H S Q V K D V M T G N P E A L Q PE A V K K A L G E D R E L F E K V H G Q V R N L M M G N P E V L K PE S V K K I L R E D P V L Y Q K I H M Q V K E L M T G H T E I I S S P T EE T V K K A L R E D S A L Y S K I Q A Q V K E L M S G P A E I I N GE T V K K A L R E D S A L Y S K I Q A Q V K E L M S G P A E I I N GE A V K K A L R E D H A L F Q Q I Y M Q V K E F M T G K V E I I S G G KE A V K K A L R E D H A L F Q Q I Y M Q V K E F M T G K V E I I S G G KE A V K K T L R E D S A L L S T I R K Q V R E M M N P S P D V V D T A VE N V K K L L K E D E T L R N T I R Q Q V R D M L T G A P T AE N V K R L L K E D E T V R N T I R Q Q V R D T L T G T P T ED A V R T V F L D N P E M Q D E I E L K I R Q K V Q L S G V P A A M E A K E L E E E E AE A A K E M I R D N E E L A E E L T E K I R E A I R N K H SD A A K V C I K D N P E L A E E L E G L I F E A L N K KD A A K Q C I A D N P E L A E E L E G L I F E K L R E H KT N S V S Y L M E N P D I L D Y L E Y T I R K K H N L I I P D Y L I E K F E S N S S K G K K V K S E E L Q E NS N A V Q F L K D N P E I A G E I E R R I R E K Y G L L S V E K E E Q R K E K K S S G E E A SN N S L D Y L L N H P E L L D E I E E K I R E K H G L L K K G E K S E E E S L ES N A V N Y L V E H S E V A D E I E K R I R E K Y L A K P D Q V S Q E K D G E N E K L Q K Q A T KE N V L A L L E E N A A Q A Q E I E I K V R E K I A S G A A V P A A A V A A P D E G D D D L G D E E VD N L V V Y L K E N P E L L A E V E A A V R E K L S A G V T V S A S R P A A M E E G E Q E E N E D A V F V E E E FD N L V V Y L K E K P E I L A E V E A A V R E K L S A G V T V S A S R P G G I E E G E Q E E V E A P I F A D E E FD N A M K Y L E E N P E I A A T I D Q Q V R E K L S L V N A V F P V E T E D G A E E Q G E D G D FD N A V K Y L E E N P D V A A I V T Q K V R E N L D M S S M G F G D E H H T T E E ED N T I T H L E E H P D F R A T V E Q Q V R E K L A L G A Q V S A N T V G A A P A K T A E D T D SD N A I K Y L E E K P E F S E Q I K Q Q V R E K L D K G A V V S A N S V A K N N E D D E D E D V E E E ED N A I K Y L E E K P E F A E E I K K L V R E K L D K G A V V S A N S V A K A S E E D E E E E V D L E P E EA V F G E V G L A G E V R S T Q M A L R R A E E A G R A G Y K R L V V P P G L D G Q A G V K S V E E A V K A V W R

Figure 9S

Chl. vibrioforme

Pld. luteolum

Chl. phaeobacteroides BS1

Ptc. aestuarii

Chl. chlorochromatii

Pld. phaeoclathratiforme

Chl. limicola

Chl. phaeobacteroides

Chl. tepidum

Cab. thermophilum

Cfx. aurantiacus

Cfx. aggregans

Rhb. sphaeroides CrtF

Rps. palustris CrtF

Rvx. gelatinosus CrtF

A. thaliana COMT

100

100

63

76

100

53

62

100

99

93

Figure 10S

Cab. thermophilum BchUCfx. aurantiacus BchUCfx. aggregans BchUChl. vibrioforme BchUPld. luteolum BchUChl. phaeobacteroides BS1 BchUPtc. aestuarii BchUChl. chlorochromatii BchUPld. phaeoclathratiforme BchUChl. limicola BchUChl. phaeobacteroides BchUChl. tepidum BchURps. sphaeroides CrtFRps. palustris CrtFRvx. gelatinosus CrtFA. thaliana ComT

1 42 1 44 1 44 1 51 1 28 1 28 1 28 1 29 1 28 1 28 1 28 1 28 1 62 1 58 1 70 1 51

M S N P V S T A A E A L S T V R E E V L Y E Q Y L R - - - - A C D M T Y R G T V D M F V L KM T T V P S V D A T K T N G T D L T Q H D L F A A T N R - - - - A Y D M V F K G T V D F F V I KM T T A P S I D A T T T A G T A L E Q Q E L F A A T N R - - - - A Y D M V F K G T V D F F V I K

M L P E P A A C E R L F L S I N C S Q Y M T M M N D I Q L L Q Q N E R - - - - A N E L I F K G L V E F G C F KM N E I A L L K Q N H R - - - - A N E L I F K G L V E F G C F KM N D N E L L K R N H R - - - - A N E I L F K G L V E F G C F KM N D N E L L K Q N H R - - - - A N E I V F K G L V E F G C F K

M M N T N E L L N Y N H R - - - - A N E L V F K G L V E F G C I KM D A N E L L K Q N H R - - - - A N E L I F K G L V E F G C F KM N D L E L L K Q N H R - - - - A N E L I F K G L V E F G C F KM N D I E L L Q Q N H R - - - - A N E L C F K G L V E F G C F KM S N N D L L N Y Y H R - - - - A N E L V F K G L I E F S C M K

M S A L R P P A R G G L G L S R L G V R L A G S R R F Q L I A E R V P F L R R I G R R E G E E L F D I V A G F V H S Q V L YM V S L S L R D R L L G W R D S I L S N P R F Q R F A A V F P L M R P V A R R R A V A M F D L V A G F V Y S Q I L L

M D R A E T R P E A G P R R S P T L A E R F R D W K H R T I A R P G F Q K W A A A F P L T R P V A R K H A R E L F D L L A G F V Y S Q I L LM I S L Q T S G G S S E E E D M L L A I Q L G G L N F V P Y I V K T A R - - - - - E L D L F E I M A K A R P L G


43 108 45 108 45 108 52 115 29 92 29 92 29 92 30 93 29 92 29 92 29 92 29 92 63 132 59 128 71 140 52 120

A A Y E L G L F R H L A D Q P Q S V E A L A A A T G S R L N R L V K F T D M L V E M E L L A K D A E G R L T L T P F A R Q F F L P P - - - -A A K D L G L F D L L A S A P R T L A D L A T L T E T V P P R L E K F L I T M E Q I G L V T R Q G D - A W Q L T P F A D Q F F A D - - - - -A A K D L G L F D L L A A E P R T L A D L A T L T E T V P P R L E K F L I T L E Q V G L V A R D A D - R W K L T P F A E Q F F A D - - - - -A A L E L D L F T H M A G S P K E A E T L A D D C Q A I P S R F V M L L E A L R Q I G I T L E S D G - K W S L T P L A R A M F V P - - - - -A A L E L D L F A L L A D G A K D T A T L A R E A G A V P P R L G M L L E A L R Q I G V T T E N A G - Q W D L T P M A R T M F V P - - - - -A A L E L D L F T E L A E E A K D A E T L S S A L E A V P Q R L E M L L E A L R Q I G I V R I E N G - K W A L T D F A R S M F V P - - - - -A A L E L D L F K H L A G E A K D V E S L A T S I E A V P Q R L A M L L E A L R Q I G I T R Q E D G - K W S L T P F A S N M F V P - - - - -A S L E L D L F T H L A G E A K D T E T I A A N V G A I P Q R L V I L L E T L A Q I G V L A K N D G - K W S L T P F A A T M F L P - - - - -A A L E L D L F T L L A D E A K N V E T L A A S A G A I P Q R L I M L L E A L R Q I G V T A E T G G - K W S L T P F A Q K M F L P - - - - -A A L E L D L F S Q L A D E A K D T G T L A A A T G S V P P R L G M L L E A L R Q M G V T N E D A G - K W S L T P S A K A M F V P - - - - -A A V E L D L F S L L A D E P K D T E D L A S S V G A V P M R L G M L L E A L R Q I G I T E E R D G - K W A L T S F S K A M F V P - - - - -A A I E L D L F S H M A E G P K D L A T L A A D T G S V P P R L E M L L E T L R Q M R V I N L E D G - K W S L T E F A D Y M F S P - - - - -A L V E L R V L H L V A E G P Q T V Q A L A A A T G L A P E R M Q F L L Q G G A A L K L L K P R R Q R Q F H L G G P G A A F L A V P G V Q AA C V Q L R L F D I V A E R P A T A E D L A A Q L S L P V D S L R V L L D G A V A L R L L Q M R S G H R Y G L G Q L G A E L R G N P G V L AA C V R L G V F K M L A D G P L P L A T V A E R C G L S L E A A R R L V A A A D S L E L L D L Q S G D L V T L G R L G A P L V A N E A I T AS Y L S P V D L A S M A - A P K N P H A P M M I D R L L R F L V A Y S V C T C K L V K D E E G R E S R A Y G L G K V G K K L I K D E D G F S


109 176 109 176 109 176 116 183

93 160 93 160 93 160 94 161 93 160 93 160 93 160 93 160

133 195 129 193 141 209 121 188

N P N N E N L T M E P F V R Y A I T I M E R Y H L H L A K V I R G E M D F T A L T P W P P - - R T R E D S A F Y E E I H R S N N H F V R N LP E R H R N M T M V P F V D Y I A D L I E N Y Y L R L A D V V R G K V D F T S I V P H P P - - R T R E D S L F Y E T L H R S N I Q L F T E LP D R H R N M T M V P F V D Y I S D L I E N Y Y L R L A D V V R G K V D F T S I V P H P P - - R T R E D S L F Y E T L H R S N I Q L F V E LQ A D Q P N M Y M V P V G K A M A H L S E N Y Y L K I A D A V R G K H E F R A E V P Y P P - - I S R E D N L Y F E E I H R S N A Y F A I K LA A D Q P N M F M V P V A K A M A H L A D S F Y L K M A D A V K G N L N F K G E V P Y P P - - V T R E D N W Y F E E I H R S N A H F A I K LS D E H P N L Y M T P V A K A M A Y T A E N F Y M H M A E A V R G K L D Y K G E T S Y P P - - K T R E E N L Y F E D I H R R N A H F S I K LN D E H P N L Y M S P V A E A M A Y T A E N F Y M H M A Q A V K G K L D Y K G E T S Y P P - - K T K E E N L Y F E E I H R R N A H F S I K LN N E L P N L Y M M P V T K A M A H L S E N F Y L K I A D A V R G N H I F K A E V P Y P P - - M T R E D N W Y F E E I H R S N A H F S I L LN H E N Q N L Y M I P V A K A M A N L S E N F Y L Q I A D A V R G N M N F K G E V A Y P P - - V T R E D N L Y F E E I H R S N A Y F A I M LD A E H P N L Y M I P V A K A M A H L S D N F Y L K L A D A V K G Q L N F K G E V P Y P P - - V T R E D N W Y F E E I H R S N A H F A I K LN S E H P N M Y M T P V A R A M V H L S D N F Y M D L A S A V K G T M N F K G E V P Y P P - - V T R E D N W Y F E E I H R S N A H F A I K LT P K E P N L H Q T P V A K A M A F L A D D F Y M G L S Q A V R G Q K N F K G Q V P Y P P - - V T R E D N L Y F E E I H R S N A K F A I Q LM W G H H H V L Y R D L A D P V A F L K G E T E P E L A R F W P Y V F G A G G A T D P - - - - - - - E V T A K Y S R L M T E S Q G L V A E DL V A H H A M L Y R D L A D P V A L L R A P R G G G E L A A Y W A Y V R G E R P G E - - - - - L P A D A I A S Y T A L M A A S Q P M I A R EM V E H H A T L Y G D L T D P V A L L R G - Q G R P A M A G Y W P Y A A A A E A G P G Q P A T L A S E K V A E Y S K L M T A S Q P L V A R EI A P Y V L A G C T K A K G G V W S Y L T E A I Q E G G A S A W E R A N E A L I F E Y M K - - K N E N L K K I F N E S M T N H T S I V M K K


177 245 177 244 177 244 184 251 161 228 161 228 161 228 162 229 161 228 161 228 161 228 161 228 196 263 194 261 210 277 189 250

L V E E A D - L E G V T H L L D V G G G N G D I A I A L A K R F P S I E R I T L I N L P S A L D I V R D N V A A H G L S D R I V P V A L D ML I K R A R - L A D V Q T L I D V G G G I G D I A A A L C Q A F P Q L K - V T L I N L P S A L D L V R E N V A A K G L A D R I T P V A I D ML V K R A R - L A D V Q T L V D V G G G I G D I A A A L C Q A F P N L K - V T L I N L P S A L D L V R E N V A A K G L S D R I T P V A I D ML L E A A E - L S G T K N L V D V G G G I G D I S A A L L K K Y P E L N - S T I L N L P G A I G L V D E N A A A Q G V G G R L K G A A V D IL L E E A R - L E G T K T L V D V G G G I G D I S A A L L K K H P Q L D - S T I L N L P G A I G L V D E N A V A Q G V G G R L R G A A V D IL L E E A D - L Q N N K K M V D V G G G I G D I S A A L C N K F S Q L D - T T I L N L P G A I E L V N E N A A E K G L A D R L R G A A V D IL L E E A R - L D D T K K M I D V G G G I G D I A A A L C K H Y P N L D - T T I L N L P G A I E L V D E N A A E K G L G D R L R G A A V D IL L E E A N - L S N V K T L V D V G G G I G D I S A A L L Q K Y P Q M D - S T I L N L P G A V E L V N E N A T E K G L A D R L R G S V V D IL L E E A D - L E T A K K L V D V G G G I G D I S A A L L K K F P Q L H - S T I L N L P G A V E L V N E N A V E K G V D N R L K G A A V D IL L E E A D - L S A A K T M V D V G G G I G D I S A A L L Q K F T N L Q - S T I L N L P G A I E L V N E N A I E K G V G D R L K G V A V D IL L E E A D - L S A A K T L V D V G G G I G D I S A A L L K K Y T G L Q - S T I L N L P G A V P L V N D N A A E K G V A D R L R G A V V D IL L E E A K - L D G V K K M I D V G G G I G D I S A A M L K H F P E L D - S T I L N L P G A I D L V N E N A A E K G V A D R M R G I A V D IA L R L V D - L M G V R R L M D V G G G T G A F L A A V G R A Y P L M E - L M L F D L P V V A E A A P Q R L T E A G L A G R F T V H G G S FV L H V Y S - F R D H R C L L D V G G G D G S F L S A V A A Q A P Q L R - C M L F D L P A V A E K A A A R F E A N G L K G R A T A V G G S FV I A A Y R - F G R H K V L M D V G G G E G A F V R C V A A E V P G V E - L R V F D L P A V A E R A N E N F Q R W G L A G R A Q A F G G D FI L E N Y I G F E G V S D F V D V G G S L G S N L A Q I L S K Y P H I K - G I N F D L P H I V K E A P Q I H G V E H I G G - - - - - - - D M


246 314 245 312 245 312 252 319 229 296 229 296 229 296 230 297 229 296 229 296 229 296 229 296 264 333 262 331 278 347 251 318

Y R D P Y P A - C D A V L F A R I L Y P F N A Q L C A M L L G K A H A A L P P G G R V V V A D M L L R D E R G L A N Y D Y L A H F L T S I GY R E P Y P P - G D A V L F S R I L Y P M N A Q F C T M L L R K A Y D A L P S G G R I L I L D M V I S D P N - H P N Y D Y L T H Y L C A I GY R D P Y P P - G D A V L F S R I L Y P M N A Q F C S M L L K K A Y D A L P S G G R V L I L D M V I S D P Q - H P N Y D Y L T H Y L C A I GY K E A Y P S - A D A V M F C R I L Y S A N E Q L T G M M C R K A Y D A L P A G G R I L I L D M I V D N P E - E P N F D Y L S H Y I L G A GY K D A Y P A - A D A V M F C R I L Y S A N E Q L T E M M C R K A Y D A V P A G G K V L I L D M I V D D R E - N P N F D Y L S H Y I L G A GY R D E Y P A - A D A V M F C R I L Y T A N E Q L T E M M C T K A F N A L E A G G K L L V L D M I V D E Q D - N P N Y D Y L S H Y I M G I GY R D E Y P A - T D A V M F C R I L Y S A N E Q L T E M M C T K A F N A L S P G G K V M V L D M I V D D P S - N P N Y D Y L S H Y I M G I GY K E E Y P K - A D A V M F C R I L Y S A N E Q I T S M M C K K A L D A L Q E G G K V L I L D M I I D E P E - D P N F D Y L S H Y I L G A GY K D A Y P E - A D A I M F C R I L Y S A N E Q L T T M L C K K A Y D A L T P G G K V L I L D M I I D D P E - N P N F D Y L S H Y I L G A GY K D V Y P K - A D A V M F C R I L Y S A N E Q L S E M M C R K A F E A L P S G G K V L I L D M I I D D P E - S P N F D Y L S H Y I L G A GY K E A Y P Q - A D A V M F C R I L Y S A N E Q L T D M M C R K A F E A L S A G G K L I I L D M V I D D P E - T P N F D Y L S H Y I L G A GY K E S Y P E - A D A V L F C R I L Y S A N E Q L S T I M C K K A F D A M R S G G R L L I L D M V I D D P E - N P N F D Y L S H Y I L G A GR D D P L P L G A D A I S L V R V L F D H S D E T V K L L L H R V R E A L P A G G R V I V A E A M S G G A R P H R E T D T Y M A F Y T A A MT T D P L P E G A D I V S L V R V I H D H D D E V V E A L L R A V H K A L P D N G K L L I A E P I A G L P G T A A I S D A Y F A F Y L Y A MF A D E L P R G A D V I S L V R V A F D H P D E R V L T L F K N V R R A L P D N G T L V L A E A M A E V P G V E P I G D A Y F G F Y L L A MF - D E I P R - G E V I L M K W I L H D W N D E K C V E I L K N C K K A L P E T G R I I V I E M I V P R E V S E T D L A T K N S L S A D L T


315 356 313 354 313 354 320 362 297 339 297 339 297 339 298 338 297 339 297 339 297 339 297 338 334 379 332 373 348 390 319 363

V D P A I M T F K R Q - - D E Y F D V F K Q L G F I D A R K V E R H G H V L Y Q A R K AM G F S V L E F K D H - - A V Y P D L L R Q V G F T D V T L D E G Y E H V L Y Q A V K PM S F S V L E F K D H - - A I Y P D L L R Q I G F T D V T F D E G Y D H V L Y Q A V K PL P F S V L G F K S Q - - E S Y R G I L E E I G F S D I S I T R R Y N H L L C Q A V K K GM P F S V L G F K S Q - - E A Y R Q I L E S I G F T D V R M V R K Y D H L L C E A V K P AM P F S V L G F K E Q - - S N Y K N I L E K I G F S D V R M V R R Y D H L Y V E A V K P SM P F S V L G Y K D Q - - D N Y K H I L E K I G F T D V R M V R R Y D H L F V E A V K P AM P F S V L G F K Q Q - - E R Y K E L L E A I G F R D V R I V R K Y G H L L C E A V KM P F S V L G F K Q Q - - S A Y K E I L E S L G F R D I R T V R K Y G H L L C E A V K A LM P F S V L G F K P Q - - N A Y K E I L E K I G F T G V R I V R R Y D H L L C E A V K P AM P F S V L G F K P Q - - S A Y K E I L E A I G F T D V R M V R K Y D Q L L C E A V K P AM P F S V L G F K E Q - - A R Y K E I L E S L G Y K D V T M V R K Y D H L L V Q A V K PR T G R V R S A A E I - - A E L L T G Q G F S E I K I F P G L R P Y V A S A V T A V R P S D A PG S G K A R S F P R - - - - - L Q A M L E N A G F S Q V T L H P V P M P L V A S V I T A S K RG R G R P R S A A R - - - - - L T Q M L H E A G F A S V R P L S T H M P L Q A G V L V A R C T AM M S L T S G G K E R T K K E F E D L A K E A G F K L P K I I Y G A Y S Y W I I E L Y P N

Figure 11S

Figure 12S

AcsF Alignments

Cab. thermophilum AcsFCfx. aggregans AcsFCfx. aurantiacus AcsFRfx. sp. RS1Rfx. castenholzii AcsFRps. palustris AcsFRb. sphaeroides AcsFRb. sphaeroides 2.4.1 AcsFSynechocystis sp. 6803 AcsFG. violaceus AcsFNostoc sp. 7120 AcsFPor. yezoensis AcsFA. thaliana AcsF

10 20 30 40 50 60 70 80 90 100 110M I A T E S V I R E T K A A A A D K S L I S P R F Y V T D Y K K I N A L R F D H - - - - - M A E A F K A M H K R F A D D P N K H F

M F W I N P L M M E M P G E F S R T T R H A L K E A I L S P R F Y T T D F K A I D K L E I D R N G - - - L R D E F D W I R D E F A Y D Y N R T HM F W I N P L M M E M P G E F S R T T R H A L K E A I L S P R F Y T T D F K A I D R L E I D R N G - - - L R D E F D W I R D E F A Y D Y N R T H

M M N I P G E Y T P T T R H A L R E A I L N P R F Y T T D F R A I D R L N V A H - - - - - M R D E F D W I R N E F E V D Y N R K HM I N M P G E Y S P T T R H A L R E A I L N P R F Y T T D F R A I D R L N V A H - - - - - M R D E F D W I R N E F E F D Y N K K H

M I P M E G G A Q G A L R T N S R P A I K G S V D S L N I A K E D T I L T P R F Y T T D Y A A M D K L D V S L - - - - - V R S E W N A M M N E M R A D Y N K A HM N A S P G D L S P A L D P E A V A D T T A M A T E T H I L N P R F Y T T D F D E L D R V D V T P - - - - - I R A Q W D E L I A E M K A D P N K G HM N A P A G G L S P A L T P E A V A D T T A M A T E T H I L N P R F Y T T D F D E L D R V D V T P - - - - - V R K D W D A L I A E M K A D P N K A H

M V N T L E K P G F D E I R P G V K T P A K E T I L T P R F Y T T D F D E M A K M D I S P N - - - - - E D E L R A I L E E F R V D Y N R H HM V D A L R E A N T A S Q S E R R E T L L T P R F Y T T D F E A L A K L D I S A M - - - - - E A D F E A L L A E F Q A D Y N R E H

M V D S L K K P G F E E I R P G V K V P A K E T L L T P R F Y T T D F D E M A R M D I S V N - - - - - E D E L L A I L E E F R T D Y N R H HM Q T T I N N G Q T S S K E T L L T P R F Y T T D F E E M A N M D I S G N - - - - - Q E D F L A I L E E F R A D Y N S E H

M A A E M A L V K P I S K F S S P K L S N P S K F L S G R R F S T V I R M S A S S S P P P P T T A T S K S K K G T K K E I Q E S L L T P R F Y T T D F E E M E Q L F N T E I N K N L N E A E F E A L L Q E F K T D Y N Q T HM A A E M A L V K P I S K F S S P K L S N P S K F L S G R R M . E T T . A . . E . I L . P R F Y T T D F . . D . L D . . N G K N L R . E F . A I . E F . . D Y N . H


120 130 140 150 160 170 180 190 200 210 220F R R D D - - - D P D F D Q D F S H L P P S F K D F L I R G C I G E F S G A L L Y K E I A D R I E D - - P I I S D T Y R L M A R D E G R H S G F I R L A M R D I N T D L D L Q M L Q K E K E F V S M H P K I I L Y T T Y L SF R R T D E F L Q N F D D M P E - - - R D A F I D F L E R S C T A E F S G C L L Y A E M V K H L H - - D P T L R A I F K Y M S R D E G R H A G F L N R T M A D L N V A L D L T V L Q K R K K Y T Y F Q P K F I F Y S V Y L SF R R T D E F L Q D F D D M P E - - - R D S F I D F L E R S C T A E F S G C L L Y A E M V K H L H - - D P T L R A I F K Y M S R D E G R H A G F L N R T M A D L N V A L D L T V L Q K R K K Y T Y F Q P K F I F Y S V Y L SF V R N D E F L A N F D D M P A - - - R D L F I E F L E R S C T A E F S G C L L Y A E M V K H L H - - D P T L K A I F R C M S R D E G R H A G F L N K T M A D L G V E M N L Q V L H T R K K Y T Y F Q P K F I F Y S V Y L SF V R N E E F L A N F D D M P A - - - R D L F I E F L E R S C T A E F S G C L L Y A E M V K H L H - - D P T L R A I F R C M S R D E G R H A G F L N K T M A D L G V E M N L Q V L H T R K K Y T Y F Q P K F I F Y S V Y L SF K K T D E F L E S D L D K L P P E L L I E F K D F L V S S L T A E F S G C V L Y S E I K K R I K - - N P E I R E L F G L L S R D E A R H A G F I N E I L K D H G I G V D L S F L T K V K K Y T Y F R P K F I F Y A T Y L SF K K T D A W D R I D W E A M D P A L R V E F I D F L V S S C T A E F S G C V L Y K E M K R R G S - - N P D I C E L F S Y M A R D E A R H A G F I N D A L R E A G V A V N L G F L T K A K K Y T Y F R P K F I Y Y A T Y L SF K K T E A W D R I D W D G M D P A L R V E F I D F L V S S C T A E F S G C V L Y K E M K R R G S - - N P D I R E L F N Y M A R D E A R H A G F I N D A L R E A G V A V N L G F L T K A K K Y T Y F R P K F I Y Y A T Y L SF V R N E S F N K S W D H I D G E - K R Q L F V E F L E R S C T A E F S G F L L Y K E L G R R L K N K N P L L A E C F N L M S R D E A R H A G F L N K A M S D F N L S L D L G F L T K S R K Y T F F K P K F I F Y A T Y L SF Q R D A G F V E Q A H Q I D P Q - W R A L M L E F L E R S C T S E F S G F L L Y K E I S R R L K A A N P L L A E C F K A M S R D E A R H A G F L N K A M G D Y D L G L D L S N L A K Q K K Y T F F K P K F I F Y A T Y L SF V R D A E F E K S W D H I D G D - T R R L F V E F L E R S C T A E F S G F L L Y K E L G R R L K D K S P V L A E C F N L M S R D E A R H A G F L N K A M S D F N L S L D L G F L T K S R N Y T F F K P K F I F Y A T Y L SF I R D E E F N Q S W S N L E H K - T K S L F I E F L E R S C T A E F S G F L L Y K E L S R K L K D R N P V I A E C F L L M S R D E A R H A G F L N K A I G D F N L S L D L G F L T K S R K Y T F F S P K F I F Y A T Y L SF V R N K E F K E A A D K L Q G P - L R Q I F V E F L E R S C T A E F S G F L L Y K E L G R R L K K T N P V V A E I F S L M S R D E A R H A G F L N K G L S D F N L A L D L G F L T K A R K Y T F F K P K F I F Y A T Y L SF R . E F . D D . L R . F I E F L E R S C T A E F S G C L L Y K E . . R R L . N P L E . F . M S R D E A R H A G F L N K A M D . N . . L D L F L T K K K Y T Y F . P K F I F Y A T Y L S


230 240 250 260 270 280 290 300 310 320 330E V I G Y F R Y I N I Y S H L E A H P E Y R Y H P I F K Y F G G W C Q D E L K H G D F I G L Q L E A Q K H L Y L S G G I N R L L I R F F S L A V Y V T M W L R D L K A Q E F Y A S I G L N W R E Y D L K V I R E T N A Y A QE K I G Y S R Y I N I Y R H L E Q N P Q Y C I H P I F K W F E Q W C N D E Y R H G E F F A L L M R S Q P E M L E G - - A N R R W I R F F L L A V Y A T M Y L N D A R R A N F Y A A L G L N W R D Y D Q K V I R L C N D I A TE K I G Y S R Y I T I Y R H L E R N P Q Y C I H P I F K W F E Q W C N D E Y R H G E F F A L L M R S Q P E M L E G - - V N R R W I R F F L L A V Y A T M Y L N D A R R S N F Y A A L G L D W R D Y D Q T V I R L C N D I A TE K I G Y A R Y I T I Y R H L Q K Y P Q G M I H P I F K W F E K W C N D E Y R H G E F F S L L M R S Q P D L L R G - - G N L R W I R F F L L A V Y A T M Y L N D A R R A G F Y Q A L G L D W R D Y D Q R V I R L T N H I A TE K I G Y A R Y I T I Y R H L Q H H P Q G M I H P I F K W F E K W C N D E Y R H G E F F S L L M R S Q P D L L R G - - G N L R W I R F F L L A V Y A T M Y L N D A R R A G F Y E A L G L N W R D Y D Q R V I R L T N H I A TE K I G Y A R Y I T I Y R Q M E R H P E R R F H P I F K W F E R W C N D E F R H G E A F A L L M R A D P S L L R G - - V N K L W I R F F L L A V F A T M Y V R D H M R P A F Y D A L G V D A T D Y D M Q V F R V T T E I S KE K I G Y A R Y I T I Y R H L E A N P E H R F H P I F K W F K E W C N D E F R H G E A F A L L M K T D P K L T D S T - V N R L W I R F F L T A V Y S T M W V R D H A R P E F H K A L G V D I D W Y D Q E V F R K T S E I A RE K I G Y A R Y I T I Y R H L E A N P E H R F H P I F K W F K E W C N D E F R H G E A F A L L M K T D P K L T D T T - V N R L W I R F F L T A V Y S T M W V R D H A R P E F H K A L G V D I D W Y D Q E V Y R K T S E I A RE K I G Y W R Y I T I Y R H L E K N P N D C I Y P I F E F F E N W C Q D E N R H G D F F D A I M R A Q P H T L N D W - K A K L W C R F F L L S V F A T M Y L N D T Q R A D F Y A C L G L E A R S Y D K E V I E K T N E T A GE K I G Y W R Y I A I F R H L E R H P E N R I H P L F N Y F K N W C Q D E N R H G D F F G L I L K S Q P Q L L K G P - I N R L W V R F F L L S F C L T M Y L N D R K P H N L Y S A L G L D A S H Y A M E V L R Q T N L D S GE K I G Y W R Y I T I Y R H L E A H P E D R I Y P I F R F F E N W C Q D E N R H G D F F D A I M K S Q P Q M L N D W - K A K L W S R F F L L S V F A T M Y L N D I Q R K D F Y A T I G L D A R D Y D I Y V I K K T N E T A GE K I G Y W R Y I T I Y R H L E Q H P E H R I Y P I F R F F E N W C Q D E N R H G D F F A A L L K S Q P H F L N D W - K A K M W C R F F L L S V F A T M Y L N D F Q R I D F Y N A I G L D S R Q Y D M Q V I R K T N E S A AE K I G Y W R Y I T I Y R H L K E N P E F Q C Y P I F K Y F E N W C Q D E N R H G D F F S A L M K A Q P Q F L N D W - Q A K L W S R F F C L S V Y V T M Y L N D C Q R T N F Y E G I G L N T K E F D M H V I I E T N R T T AE K I G Y R Y I T I Y R H L E P E R I H P I F K W F E . W C N D E . R H G E F F . L L M . S Q P L L G . N . L W I R F F L L A V Y A T M Y L N D R F Y A L G L D R . Y D V I R T N . I A


340 350 360 370 380 390 400 410 420 430 440R V F G L R L D V D N P K F I R I L D H M A A C Y A K M L K R E A D G A S K L A L M P L Q V A M A V D Y L R L L A M K V R T D A P P P I P E Y P V A P V I K G F A T K Y K G Q Y K G Q P A M A *Q V Y P E T M N L D D P R F F G Y M D K L V E Y D R K L R E L E G R K D P V A L A Q S T R L K A A I A L R L L A I Y R L P T R K T T E A I R W K G L P G F P N Y P G P N R P Q R V A A A DQ V Y P E T M N L D D P R F F A Y M D K L V E Y D R A L R A L E S R K D P I A M A H S T R L K A A I A L R L L A I Y R L P T R K T T E A I R W K G L P G F P N Y P G P N R P Q R V V A A DQ V F P V T L P V D D P R F F R H L D A C V T Y D A Q I R A L E G R S D P I A T A Q R A R L G A A I A A R L L A T Y R L P V V P T T D A N R W Q G L E G F P N Y P G P G W K Q D A T L N Q R V I S AQ V F P V T L P V D D P R F F R H L D A C V R Y D A Q I R A L E G R N D P I A Q V Q R A R L G A G I A A R L L A T Y R L P P A P T T D A N R W K G L E G F P N Y P G P G W K Q D A S L N E R V I S AQ V F P V M I N L D D P R F L A G L E R L R K V S E K I A E - S R S Q G F V G K L K R P L Y A A S A A I A F G R L F L L P A K R N E L P R V I G L R P A WQ I F P V E L D I D H P R W K P A L N R M N A A F L R M D R G N R R G G L A G R L D K V L G G A Q A L A A F T A L Y T I P V R T N A L P A N V R L E P S YQ I F P V E L D I D H P R W K P A L R R M N E A F L R I D R G T R R G G I A G R L E K A L G G A Q A L A A F V S L Y T I P V R T H T L P E N V R L E P S YR V F P I I L D V N N P E F Y N R L E T C V S N N E Q L R A I D A S G A P G V I K A L R K L P I F A S N G W Q F I K L Y L M K P I A V D Q L A G A V RK V F P L I L D L D N P R F Q R H L D R C A D L N L Q L E A L S G K S P - - - - - - L A R L P V I A A L A G T I A Q L F L L K P V A P P A P E M V RR V F P I I L D V D S P E F Y E R L D I C V E N S E K L S A I A S S N T P K F L Q F F Q K L P V L A S T G W Q L L R L Y L M K P I D A V S A Q G A A RR V F P V A L D V D N P K F F K Y L D T C A C D N R A L I D I D N N N S P L F I K S I V K I P L Y F S L F A N L L K I Y L I K P I D S K T V W N T V RR I F P A V L D V E N P E F K R K L D R M V V S Y E K L L A I G E T D D A S F I K T L K R I P L V T S L A S E I L A A Y L M P P V E S G S V D F A E F E P N L V YQ V F P . L D V D P R F . L D . . V . L A . . . P . . . . . L A . . . . . . . . . . P . P . . . . P N Y P G P Q R V I S A

15 20 25 30 35 40 45 50elution time (min)

abso

rban

ce a

t 667

nm

1 2 3 4 4321

1a

2a

3a

4b

3b

2b

1b

Bchl cF Bchl cGG

Chl a

400 600 800-2

0

2

4

6

8

10

12

14

abso

rban

ce

wavelength (nm)

Absorbance spectrumof peak 3b

667

436

Figure 13S

Table 1S. Oligonucleotide primers used in this study. Gene Description Forward (5’ to 3’) Reverse (5’ to 3’) Cab. thermophilum 16S rRNA Ribosomal RNA used

as phylogenetic marker

GAT CCT GGC TCA GAA TC (38)

GGC TTA CAC AGG ATA CC

Anoxybacillus sp. 16S rRNA Ribosomal RNA used as phylogenetic marker

CGA AAG TCT GAC GGA GCA AC

TCA TCG TTT ACG GCG TGG AC

Bacterial 16S rRNA Ribosomal RNA used as phylogenetic marker

AGA GTT TGA TCA TGG CTC AG (46)

GGT TAC CTT GTT ACG ACT T (46)

Cab. thermophilum recA DNA repair protein used as phylogenetic marker

GCA TGG AAG GCG CTG TAG CC

CAG ACA GCC TGC TTC CTA CC

Cab. thermophilum pscA Type 1 reaction center protein

GCT TTT CGT CCT ATG CCA ACG

AGG GGT CAA ACC AAC CGT AAG G

Cab. thermophilum fmoA BChl a-binding protein

GGC AAG ATT ACG GTC GGA AAG

CCC TCA ATA GGA ACA CTA AAC CCC

Cab. thermophilum csmA BChl a-binding protein located in the baseplate of chlorosomes

GTC GTG GAC TCC ATC GTC

TCC TTC GGC TGT GAA ACG

Cab. thermophilum bchU C-20 methyltransferase for BChl c biosynthesis

TTC TCT ACG AGC AGT ATT TGC GTG

CGG TGG ATT TCC TCA TAA AAC G

Cab. thermophilum acsF Oxidative cyclase for BChl a,c biosynthesis under oxic conditions

CAG GAT TTC TCC CAT CTC CCA C

CCA ATA AAG TCG CCG TGT TTG AT

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