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Supporting Information
for
Optimized design of a nanostructured SPCE-based multipurpose
biosensing platform formed by ferrocene-tethered
electrochemically-deposited cauliflower-shaped gold nanoparticles
Wicem Argoubi1, Maroua Saadaoui1, Sami Ben Aoun*2‡ and Noureddine Raouafi*1§
Address: 1University of Tunis El-Manar, Chemistry Department, Laboratory of
Analytical Chemistry and Electrochemistry (LR99ES15), campus universitaire de
Tunis El-Manar 2092, Tunis, Tunisia and 2Department of Chemistry, Faculty of
Science, Taibah University, PO. Box 30002 Al-Madinah Al-Munawarah, Saudi Arabia
Email: Noureddine Raouafi* - [email protected];
Sami Ben Aoun* - [email protected]
*Corresponding author
‡Tel.: +966590900727; Fax.: +966148628023 (Ext. 4326)
§Tel.: +21671872600 (Ext. 273); Fax.: +21671883424
Additional experimental data
S2
Table S1: Statistical distribution of formed gold nanoparticles per SEM frame
counted for 5 SEM frames and the density of nanoparticles per square
micrometer as function of the number of cycles used for the electrodeposition.
Number of cycles 5 10 15 20
Mean number of particles
dev.
/SEM frame
45 8 43 11 64 21 26 15
Density of gold nanoparticles
(nanoparticles/m2) 5.4 0.9 5.2 1.4 7.7 2.5 3.2 1.8
4.2 10-6
4.3 10-6
4.4 10-6
4.5 10-6
4.6 10-6
4.7 10-6
4.8 10-6
4.9 10-6
5 10-6
5.95 6 6.05 6.1 6.15 6.2
Cu
rre
nt
(A)
Time (s)
Figure S1: Current-time transients recorded for Au electrodeposition on a
screen-printed carbon graphite electrode at potential of +350 mV.
S3
0
1 10-6
2 10-6
3 10-6
4 10-6
5 10-6
6 10-6
7 10-6
2 3 5 6 12 20 44
Cu
rre
nt
(A)
Time (h)
Figure S2: Optimization of the incubation time of the electrode modified in 5
mM of ferrocene derivative solution.
2 10-8
4 10-8
6 10-8
8 10-8
1 10-7
1.2 10-7
1.4 10-7
1.6 10-7
1.8 10-7
-0.1 0 0.1 0.2 0.3 0.4
30 ng/mL hIgG100 ng/mL BSA100 ng/mL goat IgGWithout addition
Cu
rre
nt
(A)
Potential (v)
(a)
2 10-8
4 10-8
6 10-8
8 10-8
1 10-7
1.2 10-7
1.4 10-7
1.6 10-7
1.8 10-7
-0.1 0 0.1 0.2 0.3 0.4
30 ng/mL (hIgG + goat IgG + BSA)
Without addition100 ng/mL (goat IgG + BSA)
Cu
rre
nt
(A)
Potential (v)
(b)
Figure S3: (a) Selectivity and (b) specificity DPV studies of the immunosensor
response, immunoresponse of the hIgG sensor to BSA and gIgG interfering
proteins.
S4
1 10-7
1.05 10-7
1.1 10-7
1.15 10-7
1.2 10-7
1.25 10-7
1.3 10-7
1.35 10-7
0 5 10 15 20 25
y = 1.04e-7 + 1.3e-8x R= 1
Cu
rren
t (A
)
Concentration of hIgG added (ng/mL)
2 10-8
4 10-8
6 10-8
8 10-8
1 10-7
1.2 10-7
1.4 10-7
-0.1 0 0.1 0.2 0.3
SampleSecond addition of hIgG 10 ng/mLFirst addition of HIgG 10 ng/ml BSA/anti-hIgG/FcD/cfAuNPs/SPCE
Cu
rre
nt
(A)
Potential (V)
(a)
6 10-9
8 10-9
1 10-8
1.2 10-8
1.4 10-8
1.6 10-8
0 5 10 15 20 25
y = 6.15e-9 + 4.75e-10x R= 0.99983
Cu
rren
t (A
)
Concentration of hydrogen peroxide added (µM)
0
1 10-8
2 10-8
3 10-8
4 10-8
5 10-8
6 10-8
7 10-8
240 260 280 300 320 340 360 380 400
Cu
rre
nt
(A)
Time (s)
H2O
2/honey
10 µM
10 µM
(b)
Figure S4: Determination of the concentrations of (a) hIgG and (b) H2O2 in real
samples of serum and honey using standard addition method.