Upload File

supplementary table 1 supplementary table 1. rt-pcr primers for analysis genedirectionsequence...

  • Home
  • Documents
  • Supplementary Table 1 Supplementary Table 1. RT-PCR primers for analysis GeneDirectionSequence TNF-αForwardCCCTCACACTCAGATCATCTTCT TNF-α Reverse GCTACGACGTGGGCTACAG
8
Supplementary Table 1 Supplementary Table 1. RT-PCR primers for analysis Gene Direction Sequence TNF-α Forward CCCTCACACTCAGATCATCTTCT TNF-α Reverse GCTACGACGTGGGCTACAG IL-6 Forward TAGTCCTTCCTACCCCAATTTCC IL-6 Reverse TTGGTCCTTAGCCACTCCTTC MCP-1 Forward CTTCTGGGCCTGCTGTTCA MCP-1 Reverse CCAGCCTACTCATTGGGATCA Vegfr2 Forward CCTGGTAGAAGATTCAGGCATTG Vegfr2 Reverse CCTCACCCTGCGGATAGTCA Tek Forward AAGCATGCCCATCTGGTTAC Tek Reverse GCCTGCCTTCTTTCTCACAC αSMA Forward CCAGAGCAAGAGAGGGATCCT αSMA Reverse TGTCGTCCCAGTTGGTGATG TIMP1 Forward GCCCTTCGCATGGACATTTA TIMP1 Reverse CCCCGATCTGCGATGATG IL-1β Forward CCAGCTTCAAATCTCACAGCAG IL-1β Reverse CTTCTTTGGGTATTGCTTGGGATC Col1α1 Forward CACGGCTGTGTGCGATGA Col1α1 Reverse TCGCCCTCCCGTCTTTG Col4α1 Forward CCAGGATGCAACGGTACAAA Col4α1 Reverse AACGTGGCCGAGAATTTCAC GAPDH Forward AGGTCGGTGTGAACGGATTTG GAPDH Reverse TGTAGACCATGTAGTTGAGGTCA

Upload: kellie-kathryn-joseph

Post on 04-Jan-2016

227 views

Category:

Documents


0 download

Report

Tags:

TRANSCRIPT

Page 1: Supplementary Table 1 Supplementary Table 1. RT-PCR primers for analysis GeneDirectionSequence TNF-αForwardCCCTCACACTCAGATCATCTTCT TNF-α Reverse GCTACGACGTGGGCTACAG

Supplementary Table 1

Supplementary Table 1. RT-PCR primers for analysis

Gene Direction Sequence

TNF-α Forward CCCTCACACTCAGATCATCTTCTTNF-α Reverse GCTACGACGTGGGCTACAGIL-6 Forward TAGTCCTTCCTACCCCAATTTCCIL-6 Reverse TTGGTCCTTAGCCACTCCTTCMCP-1 Forward CTTCTGGGCCTGCTGTTCAMCP-1 Reverse CCAGCCTACTCATTGGGATCAVegfr2 Forward CCTGGTAGAAGATTCAGGCATTGVegfr2 Reverse CCTCACCCTGCGGATAGTCATek Forward AAGCATGCCCATCTGGTTACTek Reverse GCCTGCCTTCTTTCTCACACαSMA Forward CCAGAGCAAGAGAGGGATCCTαSMA Reverse TGTCGTCCCAGTTGGTGATGTIMP1 Forward GCCCTTCGCATGGACATTTATIMP1 Reverse CCCCGATCTGCGATGATGIL-1β Forward CCAGCTTCAAATCTCACAGCAGIL-1β Reverse CTTCTTTGGGTATTGCTTGGGATCCol1α1 Forward CACGGCTGTGTGCGATGACol1α1 Reverse TCGCCCTCCCGTCTTTGCol4α1 Forward CCAGGATGCAACGGTACAAACol4α1 Reverse AACGTGGCCGAGAATTTCACGAPDH Forward AGGTCGGTGTGAACGGATTTGGAPDH Reverse TGTAGACCATGTAGTTGAGGTCA

Page 2: Supplementary Table 1 Supplementary Table 1. RT-PCR primers for analysis GeneDirectionSequence TNF-αForwardCCCTCACACTCAGATCATCTTCT TNF-α Reverse GCTACGACGTGGGCTACAG

Supplementary Figure 1

Control CDAA 4 w

Supplementary Figure 1: Transmission electron microscopic images of liver sinusoidal endothelial cells (LSECs) in control and choline-deficient, L-amino acid-defined (CDAA) 4-week mice. Arrowheads indicate fenestrae. The arrow indicates thickened and defenestrated LSECs. The asterisk indicates collagen deposits in the space of Disse. Bar = 1 μm. S, sinusoid lumen; SD, space of Disse.

Page 3: Supplementary Table 1 Supplementary Table 1. RT-PCR primers for analysis GeneDirectionSequence TNF-αForwardCCCTCACACTCAGATCATCTTCT TNF-α Reverse GCTACGACGTGGGCTACAG

Control CDAA 4 w

Supplementary Figure 2

Supplementary Figure 2: Scanning electron microscopic images of Kupffer cells (top row), hepatic stellate cells (HSCs) (bottom left panel), and collagen deposits in the space of Disse (bottom right panel) in control and choline-deficient, L-amino acid-defined (CDAA) 4-week mice. Arrowheads indicate an extended pseudopod of Kupffer cell. The uneven surface of the HSC indicates intracellular storing of lipid droplets such as vitamin A. The asterisk indicates collagen deposits in the space of Disse. Bar = 5 μm. HSC, hepatic stellate cell; K, Kupffer cell; LD, lipid droplet; S, sinusoid lumen.

Page 4: Supplementary Table 1 Supplementary Table 1. RT-PCR primers for analysis GeneDirectionSequence TNF-αForwardCCCTCACACTCAGATCATCTTCT TNF-α Reverse GCTACGACGTGGGCTACAG

Supplementary Figure 3

Supplementary Figure 3: Macroscopic photograph (top panel), hematoxylin and eosin staining (bottom left panel), and Azan staining (bottom right panel) of livers from choline-deficient, L-amino acid-defined (CDAA) 22-week mice. Asterisks indicate hepatocellular adenomas. The dashed lines depict the borders between the hepatocellular adenoma and non-neoplastic parenchyma. Bar = 200 μm.

CDAA 22 w

Page 5: Supplementary Table 1 Supplementary Table 1. RT-PCR primers for analysis GeneDirectionSequence TNF-αForwardCCCTCACACTCAGATCATCTTCT TNF-α Reverse GCTACGACGTGGGCTACAG

Control 8 w Recovery 4 wCDAA 8 w

Supplementary Figure 4

Supplementary Figure 4: Toluidine blue staining of resin-embedded liver sections for control 8-week, choline-deficient, L-amino acid-defined (CDAA) 8-week, and recovery 4-week (8 weeks of CDAA feeding followed by an additional 4 weeks of standard diet feeding) mice. Arrowheads indicate quiescent hepatic stellate cells (lipid storing cells) in the space of Disse. Arrows indicate activated hepatic stellate cells (myofibroblasts, spindle-shaped collagen producing cells). Bar = 10 μm. LD, lipid droplet.

Page 6: Supplementary Table 1 Supplementary Table 1. RT-PCR primers for analysis GeneDirectionSequence TNF-αForwardCCCTCACACTCAGATCATCTTCT TNF-α Reverse GCTACGACGTGGGCTACAG

HFD 8 w HFD 22 w

Supplementary Figure 5

Supplementary Figure 5: Transmission electron microscopic images of livers of high fat diet (HFD) 8-week and HFD 22-week mice. Arrowheads indicate fenestrae. Arrows indicate small lipid particles. The asterisk indicates collagen deposits in the space of Disse. Bar = 500 nm. S, sinusoid lumen; SD, space of Disse.

Page 7: Supplementary Table 1 Supplementary Table 1. RT-PCR primers for analysis GeneDirectionSequence TNF-αForwardCCCTCACACTCAGATCATCTTCT TNF-α Reverse GCTACGACGTGGGCTACAG

CDAA 4 w CDAA 8 w CDAA 22 w

Recovery 4 w

Supplementary Figure 6

Supplementary Figure 6: Transmission electron microscopic images of livers in choline-deficient, L-amino acid-defined (CDAA) 4-week (bar = 500 nm), CDAA 8- week (bar = 2 μm), CDAA 22-week (top right panel: bar = 5 μm, bottom panel: bar = 1 μm), and recovery 4-week (8 weeks of CDAA feeding followed by an additional 4 weeks of standard diet feeding: bar = 1 μm) mice. Black arrows indicate enlarged endoplasmic reticulum. Black arrowheads indicate autophagosomes and lysosomes (high-density organelles). White arrowheads indicate loss of microvilli in the bile canaliculus. White arrows indicate deformed mitochondria. The asterisk indicates a pseudoinclusion body. N, nucleus of hepatocyte; LD, lipid droplet; BC, bile canaliculus.

CDAA 22 w

Page 8: Supplementary Table 1 Supplementary Table 1. RT-PCR primers for analysis GeneDirectionSequence TNF-αForwardCCCTCACACTCAGATCATCTTCT TNF-α Reverse GCTACGACGTGGGCTACAG

HFD 8 w HFD 22 w

HFD 22 w

Supplementary Figure 7

Supplementary Figure 7: Transmission electron microscopic images of livers in high fat diet (HFD) 8-week (bar = 1 μm) and HFD 22-week (top right panel: bar = 500 nm, bottom left panel: bar = 5 μm, bottom right panel: bar = 500 nm) mice. A white arrow indicates a deformed mitochondrion. Black arrows indicate enlarged endoplasmic reticula. Black arrowheads indicate autophagosomes and lysosomes (high-density organelles). Hepatocyte nuclei and a bile canaliculus show normal appearance. N, nucleus of hepatocyte; LD, lipid droplet; BC, bile canaliculus.

HFD 22 w


Supplementary table 1: List and characteristics of

SUPPLEMENTARY INFORMATION - Nature · Dcn CCTTCTGGCACAAGTCTCTTGG TCGAAGATGACACTGGCATCGG ... SUPPLEMENTARY INFORMATION 2 | RESEARC Supplementary Table 2 List of primers used for human

Supplementary Data: Table of Contents

Online Supplementary Table 1 Macronutrients and their

SUPPLEMENTARY TABLE OF STANDARDS AND GAPSsiteresources.worldbank.org/.../Publications/448813-1195243379044/... · SUPPLEMENTARY TABLE OF STANDARDS AND GAPS ... Education and Training

Supplementary figure 1. Supplementary figure 1: TNF-α receptor 2 expression in the skin keratinocytes of C57 wild-type mice after TNF-α induction. (A)

Knight et al – Supplementary Table

File Name Supplementary Information Figures, 1710.1038...File Name: Supplementary Information Description: Supplementary Figures, Supplementary Table, Supplementary Note and 17 Supplementary

Supplementary Table 1

Supplementary Table 1. Characteristics of Studies Included

Supplementary Table 1. Clinicopathologic Information for ...clincancerres.aacrjournals.org/content/suppl/2008/03/31/14.7.1938.DC1/...Supplementary Table 1. Clinicopathologic Information

Supplementary Table S1: Primers for DNA sequencing (A ...download.lww.com/wolterskluwer_vitalstream_com/PermaLink/JTO/… · Supplementary Table S1: Primers for DNA sequencing (A),

Supplementary Table S1: 109 genes whose expression was ...download.lww.com/wolterskluwer_vitalstream_com/... · Radom-Aizik et al. - Supplementary material 1 Supplementary Table S1

SUPPLEMENTARY INFORMATION - Nature · | 3 SUPPLEMENTARY INFORMATION RESEARCH 5 Supplementary Table 3.Correspondence between class A …

Supplementary material: Mapping tables Table 1 Technology

Online Supplementary Document Table S1: Documents included

Supplementary Table 5: Description of interventions and

Supplementary Table I. Morpholino oligonucleotides and primer

Table of Contents: Supplementary Material

Supplementary Table 1

Supplementary materials - Welcome to ESC …eprints.esc.cam.ac.uk/3013/3/mmc1.pdfSupplementary materials Supplementary Table 1 2 Supplementary Table 2 ... Table 1: Experimental conditions

SUPPLEMENTARY MATERIAL Supplementary Table 1 … ·  · 2012-04-16SUPPLEMENTARY MATERIAL Supplementary Table 1 Enrichment of phage titers following each round of biopanning.

Supplementary Materials for€¦ · 1/31/2011  · Results are expressed ... SUPPLEMENTARY TABLE 1 n vitro conversion of CD4. CD25- peripheral T cells . SUPPLEMENTARY TABLE 2. Induction

Supplementary table 1. Supplementary table 2 Supplementary table 3

media.nature.com · 1 Supplementary Materials Supplementary Table S1 Supplementary Table S2 Supplementary Table S3 Supplementary Figure S1 Supplementary Figure S2 Supplementary Figure

Supplementary Material Table S01

SUPPELEMNTARY TABLE 1 · Web view8 Supplementary Figure S2 9 Supplementary Figure S3 10 SUPPLEMENTARY METHODS 13 References 14 GERAD1 author list Supplementary Table S1. Association

Supplementary Table 2

Supplementary Table S1 - ncbi.nlm.nih.gov

SUPPLEMENTARY MATERIALS AND METHODS, TABLE, FIGURES …

Supplementary Table 1. Immunological Characteristics of Patients

Data Descriptor Template · Web viewSupplementary Table S 2: Full list of emissions files3. Supplementary Table S 3: Full list of land-use files8. Supplementary Figures. Supplementary

Supplementary Information Using population admixture to ......Supplementary Table 10. List of identified PSVs between the three PRIM2 paralogs.....51 Supplementary Table 11. List of

Supplementary Appendix Table of contents

Supplementary Table 1