supplementary methods - images.nature.com · supplementary methods ... all the raw data files...

Supplementary Methods

Reprocessing of raw data

All the raw data files analyzed in this report have been originally generated by Steven Gygi’s

lab1. Experimental details and instruction about how to download the raw files from Tranche

(https://proteomecommons.org/) can be found in the extended version of the article that

includes supplemental information and can be downloaded with the following URL:

http://www.sciencedirect.com/science/MiamiMultiMediaURL/1-s2.0-S0092867410013796/1-

s2.0-S0092867410013796-

mmc5.pdf/272196/html/S0092867410013796/818d8142e6d9ca30c93e71532ad0ffdf/mmc5.pd

f

Raw data were processed with the MaxQuant proteomics software package (version 1.2.2.5)2,

integrated with the search engine Andromeda3. This suite of programs can be downloaded as

freeware at http://maxquant.org. The database search was performed against the mouse

International Protein Index (IPI) database (version 3.68) with the initial maximum allowed mass

deviation set to 7 ppm for peptide masses and 0.5 Da for CID fragment ions. Enzyme specificity

was set to trypsin, allowing for cleavage N-terminal to proline residues and up to four missed

cleavages. Cysteine carbamidomethylation was set as a fixed modification. The following

variable modifications were used: methionine oxidation; protein N-acetylation;

phosphorylation on serine, threonine and tyrosine residues; ADP-ribosylation (mass shift of

541.0611; composition C15 H21 N5O13P2) and phosphate-ribosylation (mass shift of 212.0086;

composition C5 H9 N5O7P) on glutamate, aspartate and arginine. In case of ADP-ribosylation,

MaxQuant automatically searched for the following diagnostic peaks4,5 in the fragmentation

spectra: adenine (mass 135.0545; composition C5H5N5), adenosine –H2O (mass 249.0862;

composition C10H11N5O3), adenosine monophosphate (AMP; mass 347.0631; composition

C10H14N5O7P), adenosine diphosphate (ADP; composition C10H15N5O7P2). Because diagnostic

ions of ribose-phosphate cannot be detected in the positive mode they have not been added to

MaxQuant.

Nature Methods: doi:10.1038/nmeth.2106

https://proteomecommons.org/

http://www.sciencedirect.com/science/MiamiMultiMediaURL/1-s2.0-S0092867410013796/1-s2.0-S0092867410013796-mmc5.pdf/272196/html/S0092867410013796/818d8142e6d9ca30c93e71532ad0ffdf/mmc5.pdf




http://maxquant.org/

Manual evaluation and assignment of MS/MS spectra

The MS/MS spectra were manually validated and filtered by the following stringent criteria: (i)

only peptides containing an internal modified arginine and fragment ions from ADP-ribose were

accepted; (ii) after MaxQuant recalibration maximum mass deviation smaller than 3 ppm; (iii)

extensive coverage of peptide backbone fragment ions; (iv) preferential cleavage N-terminal to

proline and C-terminal to aspartate and glutamate.

In addition to the diagnostic ions that are automatically annotated by MaxQuant as described

above, we manually assigned other fragment ions generated from the fragmentation of ADP-

ribose. In accordance to previous reports4,5 the following losses from the precursor ion and, less

commonly, from b and/or y ions, appear to be the most pronounced: adenine; adenine –H2O;

adenine –2H2O; adenosine –H2O; adenosine –2H2O; AMP; AMP –H2O; AMP –2H2O. These

characteristic ions pinpoint ADP-ribosylation with very high confidence. Their relative

intensities vary profoundly depending on the nature of the substrate peptide. For some spectra

this fragmentation behavior allows the presence of multiple high quality fragment ions derived

from the peptide backbone and does not reduce the confidence of peptide assignment while

for others a more pronounced fragmentation of the ADP-ribose branch makes the sequence

assignment more challenging6. The N-glycosidic bond between arginine and ADP-ribose is

remarkably stable during CID as only very few fragments derived from the loss of the whole

ADP-ribose have been observed here. On the contrary, as described previously7, the ester bond

joining the ADP-ribose to the side chain of glutamate appears to be much more labile: the most

intense fragment ion of the only ADP-ribosyl glutamate containing peptide that was identified

here is the loss of ADP-ribose from the precursor ion. This perhaps explains at least in part the

apparent underrepresentation of glutamate ADP-ribosylation in these experimental

conditions1.


Supplementary References

1 Huttlin, E. L. et al. A tissue-specific atlas of mouse protein phosphorylation and

expression. Cell 143, 1174-1189, doi:10.1016/j.cell.2010.12.001 (2010).

2 Cox, J. & Mann, M. MaxQuant enables high peptide identification rates, individualized

p.p.b.-range mass accuracies and proteome-wide protein quantification. Nat Biotechnol

26, 1367-1372, doi:nbt.1511 [pii]10.1038/nbt.1511 (2008).

3 Cox, J. et al. Andromeda: a peptide search engine integrated into the MaxQuant

environment. J Proteome Res 10, 1794-1805, doi:10.1021/pr101065j (2011).

4 Laing, S., Koch-Nolte, F., Haag, F. & Buck, F. Strategies for the identification of arginine

ADP-ribosylation sites. J Proteomics 75, 169-176, doi:10.1016/j.jprot.2011.07.003

(2011).

5 Hengel, S. M. & Goodlett, D. R. A review of tandem mass spectrometry characterization

of adenosine diphosphate-ribosylated peptides. International Journal of Mass

Spectrometry 312, 114-121, doi:10.1016/j.ijms.2011.06.003 (2012).

6 Hengel, S. M., Icenogle, L., Collins, C. & Goodlett, D. R. Sequence assignment of ADP-

ribosylated peptides is facilitated as peptide length increases. Rapid Commun Mass

Spectrom 24, 2312-2316, doi:10.1002/rcm.4600 (2010).

7 Tao, Z., Gao, P. & Liu, H. W. Identification of the ADP-ribosylation sites in the PARP-1

automodification domain: analysis and implications. J Am Chem Soc 131, 14258-14260,

doi:10.1021/ja906135d (2009).


Supplementary data

Representative fragmentation spectra of all modified peptides reported in the Supplementary Table are shown. These spectra were produced with the Viewer tool of the MaxQuant suite of algorithms and manually validated.

adpr = mono ADP-ribosylation

ri = ribose phosphate


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f2_02_w01363

5942

Acat3; Acat3; Q80X81; Q8R4V3

117.7

ITMS; CID; 1

ac

_ M N T

y₁₁

adpr

R

y₁₀

b₅

S

y₉

b₆

D P

y₇

b₈

V

y₆

b₉²⁺

V

y₅

b₁₀*

I

y₄

b₁₁*

V

y₃

S A A R _

y₃317.2

AMP348.1 y₄

404.2

428.0

y₉²⁺456.3

y₅503.3

y₆616.4

b₆²⁺644.7

y₇715.4

b₉²⁺792.3

b₁₀²⁺848.8

b₁₁²⁺898.4

y₉911.6 y₁₀

1026.6

y₁₁1113.6

b₅-H₂O1155.3

b₅1173.3

b₆-H₂O1270.4

b₆-NH₃1271.3

b₆1288.4

1349.6

1448.6

b₈1484.5

b₆²⁺-H₂O635.6

b₁₀-AMPb₁₁-AMP

400 600 800 1000 1200 1400 1600 1800

m/z (full)

020

4060

8010

012

0

Rel

ativ

e Ab

unda

nce

050

100150

200250

Intensity

400 600 800 1000 1200 1400 1600 1800

m/z (zoom)

ADP

1032.6

MH₂²⁺-adenine

1023.6

MH₂²⁺-adenine-H₂O

1014.5MH₂²⁺-adenine-2H₂O

975.6MH₂²⁺-adenosine-H₂O

926.6

MH₂²⁺-AMP

MH₂²⁺-adenosine-2H₂O966.8

MH₂²⁺-AMP-H₂O917.6


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f2_01_w01047

4613

Adss; Adss2; mCG_14946; P46664; B9EIE9; Q8C909

61.82

ITMS; CID; 1

ac

_ S

y₁₉²⁺

I

y₁₈²⁺

b₃

S

y₁₇²⁺

b₄-H₂O

E

y₁₆²⁺

b₅

S

y₁₅²⁺

b₆-H₂O

S

y₁₄²⁺

P

y₁₃²⁺

A

y₁₂²⁺

A T S

y₉²⁺

L P

y₇

N

b₁₅

G D C G

y₂

adpr

R P R _

y₂272.2

b₃330.2

b₄-H₂O441.2

b₅-H₂O528.2

b₅546.2

b₆-H₂O615.3

y₉²⁺785.3

y₁₂²⁺935.9

y₁₃²⁺971.4

y₁₄²⁺1046.6

y₁₅²⁺1055.4

y₁₆²⁺1098.9

y₁₇²⁺1142.4

y₁₈²⁺1207.0

y₁₉²⁺1250.5 y₇

1358.4

b₁₅1441.7

1006.9

y₁₅²⁺-H₂O

300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700 1800 1900

m/z (full)

020

4060

8010

012

0

Rel

ativ

e Ab

unda

nce

050

100150

200

Intensity

300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700 1800 1900

m/z (zoom)

1304.1MH₂²⁺-adenine

1295.1MH₂²⁺-adenine-H₂O


1198.0MH₂²⁺-AMP

1189.2MH₂²⁺-AMP-H₂O

903.0MH₃3⁺-2H₂O


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f6_02_w01367

4532

Ahsg; Fetua; mCG_128255; P29699; O35399; Q3TIU3; Q3UEK5; Q3UEK9

74.95

ITMS; CID; 1

_ V G

y₁₆²⁺

Q P

y₁₄²⁺

G

y₁₃²⁺

b₆

A

y₁₂²⁺

b₇

A

b₈

G P

b₁₀

V

y₈²⁺

S P M

y₅

C P G

adpr

R I R _

adenosine - H2O250.1

AMP348.1

ADP428.0

b₆

510.3b₇

581.3

b₈638.3

y₈²⁺764.3

b₁₀834.4

y₁₂²⁺934.4

y₁₃²⁺969.9

y₁₄²⁺1005.4

y₁₆²⁺1082.4

y₅1139.4

1157.0

1147.8

1100.4

1100.4

721.9

y₁₆3⁺

020

4060

8010

012

0

Rel

ativ

e Ab

unda

nce

0500

10001500

20002500

30003500

4000

Intensity

300 400 500 600 700 800 900 1000 1100 1200

m/z (zoom)

MH₂²⁺-adenine


MH₂²⁺-adenosine-H₂O

MH₂²⁺-AMP


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f5_01_w01050

6428


92.77

ITMS; CID; 1

_ P

b₂

P A S

b₅

V

b₆

V

y₁₃²⁺

V

y₁₂²⁺

b₈

G

y₁₁²⁺

b₉²⁺

P

y₁₀²⁺

b₁₀

V

y₉²⁺

b₁₁

V

y₈²⁺

V P

adpr

R G L S

y₂

D H R _

b₂195.1


y₂312.2

AMP348.1

b₈²⁺354.2

b₉²⁺402.7

ADP428.0

b₅452.3

b₁₁²⁺501.8

b₆-H₂O533.3

b₆551.3

b₈707.4

y₈²⁺739.8

y₉²⁺789.3

y₁₀²⁺838.8

y₁₁²⁺888.4

b₁₀903.5

y₁₂²⁺936.9

y₁₃²⁺965.4

b₁₁1002.6

625.1

y₁₂3⁺

200 300 400 500 600 700 800 900 1000 1100

m/z (full)

020

4060

8010

012

0

Rel

ativ

e Ab

unda

nce

01000

20003000

40005000

6000

Intensity

200 300 400 500 600 700 800 900 1000 1100

m/z (zoom)

815.4MH3

3⁺-adenine

809.7

MH33⁺-adenine-H₂O

745.0MH3

3⁺-AMP

740.5

MH33⁺-AMP-H₂O


Source:

Scannumber:

Protein:

Peptide Score:

Method:

o05362

4002


71.15

ITMS; CID; 1

_ T

a₂

Y H D

b₅-H₂O

L

y₂₁²⁺

adpr

R H A F

y₁₇²⁺

b₁₀²⁺

S

y₁₆²⁺

P

y₁₅²⁺

b₁₂²⁺

V

y₁₄²⁺

b₁₃²⁺

A

b₁₄²⁺

S

y₁₂

V

y₁₁²⁺

E

y₁₀²⁺

S

y₉²⁺

A

y₈²⁺

S G

y₆²⁺

E T L

y₃

H S P K _

a₂237.1


y₃331.2

y₆²⁺341.7

AMP348.1

ADP428.0

y₈²⁺434.7

y₉²⁺478.3

y₁₀²⁺513.8

y₁₁²⁺557.3

b₅-H₂O612.3

y₁₂²⁺621.8

y₁₄²⁺714.8

y₁₅²⁺750.4

y₁₆²⁺799.9

y₁₇²⁺848.4

b₁₀²⁺885.3

b₁₂²⁺983.4 b₁₃²⁺

1018.9

b₁₄²⁺1062.4

y₂₁²⁺1069.5

y₁₂-NH₃1225.6

y₁₂1242.6

833.4

828.8

824.3

804.7

780.1

1053.4

b₁₄²⁺-H₂O566.1

y₁₇3⁺

595.2y₁₈3⁺

020

4060

8010

012

0

Rel

ativ

e Ab

unda

nce

01000

20003000

Intensity

200 300 400 500 600 700 800 900 1000 1100 1200 1300

m/z (zoom)

MH₄4⁺-adenine

MH₄4⁺-adenine-H₂O

MH₄4⁺-adenine-2H₂O

MH₄4⁺-adenosine-H₂O

MH₄4⁺-AMP


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f9_02_w01370

10560

Akr1a1; Akr1a4; RP23-177C18.1-001; Q9JII6; Q3UJW9; Q540D7; Q80XJ7

119.4

ITMS; CID; 1

_ A

b₂

W

y₁₉²⁺

adpr

R H P

b₆²⁺

D E P

b₉²⁺

V

b₁₀²⁺

L

y₁₁

b₁₁²⁺

L

y₁₀²⁺

b₁₂²⁺

E

y₉

b₁₃²⁺

E P

y₇

b₁₅²⁺

V

y₆

b₁₆²⁺

V

y₅

L

y₄

b₁₈²⁺

A

y₃

L

y₂

A E K _

b₂258.1

y₂276.1

y₃347.2

AMP348.1

y₄460.3

y₉²⁺470.3

y₅531.3

y₁₀²⁺534.8

y₆644.4

b₆²⁺652.7

y₇743.5

b₉²⁺815.3

b₁₀²⁺871.8

b₁₁²⁺928.4

y₉939.6

b₁₂²⁺992.9

y₁₉²⁺1049.6

b₁₃²⁺1057.4

b₁₅²⁺1155.5

y₁₁1197.7

b₁₆²⁺1205.0

b₆-H₂O1286.4

b₁₈²⁺1297.1

b₁₃3+

705.3

662.2b₁₂3+

b₁₁3+

619.4

803.7

b₁₆3+

969.7b₂₁3+903.0

b₁₉3+

926.9b₂₀3+

864.9b₁₈3+

841.5b₁₇3+

400 600 800 1000 1200 1400 1600

m/z (full)

020

4060

8010

012

0

Rel

ativ

e Ab

unda

nce

050

100150

200250

300

Intensity

400 600 800 1000 1200 1400 1600

m/z (zoom)


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f6_02_w01367

7239

Alb; Alb1; Alb-1; alb; mCG_1705; P07724; Q546G4

67.4

ITMS; CID; 1

_ L

y₁₈²⁺

b₂

C

b₃

A

y₁₆²⁺

b₄

I P

y₁₄*

b₆

N L

adpr

R

b₉²⁺

E N

y₉

b₁₁*

Y G

y₇

b₁₃²⁺

E

y₆

b₁₄*

I

y₅

A

y₄

b₁₆²⁺

D C C T K _

b₂274.1

b₃345.2

AMP348.1

ADP428.0

b₄458.2

y₄568.2

b₆669.3

y₅683.2

y₆754.3

b₉²⁺804.8

y₇867.4

b₁₃²⁺1036.4

y₉1053.4

b₁₆²⁺1186.0

y₁₆²⁺1241.0

y₁₈²⁺1333.0

b₁₁-AMP1538.7

b₁₄-AMP1837.8

y₁₄-AMP1922.8

1402.2

1393.1

MH₂²⁺-adenine



MH₂²⁺-adenine-H₂O-NH₃1384.6

1345.2

1296.2

MH₂²⁺-AMP

1232.1

y₁₆²⁺-H₂O

1178.2

b₁₆²⁺-H₂O

1173.5

y₁₆²⁺-adenine

1067.2

y₁₆²⁺-AMP

1116.0

y₁₆²⁺-adenosine-H₂O

400 600 800 1000 1200 1400 1600 1800

m/z (full)

020

4060

8010

012

0

Rel

ativ

e Ab

unda

nce

0500

10001500

2000

Intensity

400 600 800 1000 1200 1400 1600 1800

m/z (zoom)


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f2_02_w01363

7393

Apeh; Apeh; Q8R146-1; Q8R146; Q8R146-2; Q8R146

88.77

ITMS; CID; 1

ac

_ M E

adpr

R

y₁₄²⁺

Q

y₁₃

V

y₁₂

b₆

L

y₁₁

b₇²⁺

L

y₁₀²⁺

b₈-NH₃

S

y₉

b₉

E

b₁₀²⁺

P Q E A

y₄

A

y₃

b₁₅²⁺

A

b₁₆²⁺

L Y R _

AMP348.1

y₃

451.3 y₉²⁺509.8

y₄

522.3

y₁₀²⁺574.3

b₆²⁺670.7

y₁₂²⁺674.3

b₇²⁺727.3

y₁₄²⁺780.4

b₉²⁺835.3

b₁₀²⁺883.8

b₆-AMP993.4

y₉1018.5

b₁₅²⁺1118.9

b₁₆²⁺1175.5

y₁₁1234.6

b₆1340.5

y₁₂1347.7

y₁₃1460.7

b₈-NH₃1523.6

b₉1669.6


1267.2


1170.5

MH₂²⁺-AMP-H₂O

MH₂²⁺-AMP

1161.4

826.3b₉²⁺-H₂O

400 600 800 1000 1200 1400 1600 1800

m/z (full)

020

4060

8010

012

0

Rel

ativ

e Ab

unda

nce

010

2030

4050

6070

Intensity

400 600 800 1000 1200 1400 1600 1800

m/z (zoom)


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f6_01_w01051

8379

Arg1; Q61176; Q8K3K5

114.83

ITMS; CID; 1

_

y₁₂²⁺

V

y₁₁*

a₂

M

y₁₀²⁺

b₃

E

y₉²⁺

b₄

E

y₈²⁺

T

y₇²⁺

b₆

F

y₆²⁺

S Y

b₉

L

y₃-NH₃

L G

adpr

R K _

a₂203.1

AMP348.1

b₃360.2

ADP428.0

b₄489.2

b₉²⁺550.7

y₆²⁺645.8

y₇²⁺689.3

b₆737.3

y₈²⁺762.8

y₉²⁺813.3

y₁₀²⁺877.9

y₃-NH₃884.3

y₁₁²⁺942.4

y₁₂²⁺1007.9

b₉-H₂O1082.5

b₉1100.5 y₁₁-AMP

1536.7

y₁₁²⁺-H2O933.2

990.1

981.4

200 400 600 800 1000 1200 1400 1600

m/z (full)

020

4060

8010

012

0

Rel

ativ

e Ab

unda

nce

00.5

11.5

22.5

Intensity [10e4]

200 400 600 800 1000 1200 1400 1600

m/z (zoom)

MH₂²⁺-adenine



Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f6_02_w01367

8940

Arhg; Rhog; Sid10750; mCG_23419; P84096; B2KKU6; Q3UDZ1

83.11

ITMS; CID; 1

_ T

y₁₇²⁺

V

b₃

N

y₁₅²⁺

b₄

L

y₁₄²⁺

N

y₁₃²⁺

b₆

L

y₁₂

b₇

W D

y₁₀²⁺

T

y₉²⁺

A G Q E

b₁₄-NH₃

E

b₁₅-NH₃

Y

y₃

b₁₆²⁺

D

adpr

R L R _

b₃315.2

AMP348.1

b₄428.3

b₆-H₂O637.4

y₃-AMP638.3

b₆655.4

b₇841.5

y₉²⁺853.8

y₁₀²⁺889.3

b₁₆²⁺925.4

y₁₂²⁺997.4

y₁₃²⁺1090.4

y₁₄²⁺1146.9

y₁₅²⁺1204.0

y₁₇²⁺1317.5

b₁₄-NH₃1554.7

b₁₅-NH₃1717.8

y₁₂1993.7

1350.0

1341.3

1292.7


1244.2MH₂²⁺-AMP

1235.2


1135.5y₁₄²⁺-H₂O

1081.3

y₁₃²⁺-H₂O

878.8y₁₇3⁺

873.2y₁₇3⁺-NH₃

400 600 800 1000 1200 1400 1600 1800 2000

m/z (full)

020

4060

8010

012

0

Rel

ativ

e Ab

unda

nce

0200

400600

8001000

Intensity

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m/z (zoom)

MH₂²⁺-adenine



Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f5_01_w01050

5746

Car; Cxadr; Car; Cxadr; P97792-1; P97792; Q3TV22; P97792-2; P97792

60.62

ITMS; CID; 1

_ T Q

y₂₁²⁺

Y N

y₁₉²⁺

b₅

Q

y₁₈²⁺

b₆

V P S E

y₁₄²⁺

D F

y₁₂²⁺

E

adpr

R

y₁₀

A P

y₈

Q S

y₆

b₁₈²⁺

P

y₅

T

b₂₀²⁺

L A P A K _

AMP348.1

y₅499.3

y₁₀²⁺505.3

y₆600.4

b₅635.3

b₆-H₂O716.4

b₆-NH₃717.3

b₆734.3 y₈

784.5

y₁₂²⁺889.4

y₁₀1009.6

y₁₄²⁺1027.4

y₁₈²⁺1241.5

y₁₉²⁺1291.1

b₁₈²⁺1308.5

y₂₁²⁺1412.1

b₂₀²⁺1415.6

1541.0

1531.8

1523.0

1483.6

1474.9

1434.8


MH₂²⁺-AMP

1425.7

020

4060

8010

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200300

400500

600

Intensity

m/z (zoom)

MH₂²⁺-adenine


MH₂²⁺-adenosine-2H₂O

MH₂²⁺-adenine-2H₂O



Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f5_01_w01050

5773

Car; Cxadr; Car; Cxadr; P97792-1; P97792; Q3TV22; P97792-2; P97792

30.78

ITMS; CID; 1

_ T

y₂₂²⁺

Q

y₂₁²⁺

Y

b₄

N

y₁₉²⁺

b₅

Q

y₁₈²⁺

b₆

V P S

b₉-H₂O

E D

y₁₃

F

y₁₂²⁺

E

ri

R

y₁₀²⁺

A P Q S P

b₁₉²⁺

T L A P A K _

b₄-H₂O489.2

y₁₀²⁺505.3

b₄507.2

b₅-H₂O617.3

b₅635.3

b₆-NH₃717.3

y₁₂²⁺724.9

b₆734.3

b₉-H₂O1029.5

y₁₈²⁺1077.0

y₁₉²⁺1126.5

b₁₉²⁺1194.5

y₂₁²⁺1247.6

y₂₂²⁺1329.1

y₁₃1577.8

300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600

m/z (full)

020

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8010

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unda

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0200

400600

8001000

Intensity

300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600

m/z (zoom)


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f2_01_w01047

7927

Catnb; Ctnnb1; Ctnnb1; Q02248; Q80VE7; Q3UZT7

96.41

ITMS; CID; 1

ac

_ A

y₁₆²⁺

T

y₁₅²⁺

b₃-H₂O

Q

y₁₄²⁺

b₄

A

y₁₃²⁺

b₅

D

y₁₂²⁺

b₆

L

y₁₁²⁺

M E

y₉²⁺

b₉

L

y₈²⁺

D

y₇

b₁₁²⁺

M

b₁₂-H₂O

A M

b₁₄-NH₃

E

b₁₅²⁺

P D

b₁₇²⁺

adpr

R K _

b₃-H₂O325.2

AMP348.1

b₄-H₂O396.2

b₄414.2

ADP428.0 b₅

529.2

b₁₁²⁺631.3

b₆642.3

y₈²⁺759.8

y₉²⁺817.3

b₁₅²⁺845.4

y₁₁²⁺938.3

y₁₂²⁺1003.9

b₉1015.5

y₁₃²⁺1060.4

y₁₄²⁺1117.9

y₁₅²⁺1153.4

y₁₆²⁺1217.462

b₁₇²⁺1251.5

b₁₂-H₂O1314.6

y₇1387.5

b₁₄-NH₃1575.6

826.4y₂-H₂O

y₁₁²⁺-H₂O929.2


MH₂²⁺-adenine-H₂O1256.8

1151.2MH₂²⁺-AMP

300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600

m/z (full)

020

4060

8010

012

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Rel

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050

100150

Intensity

300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600

m/z (zoom)


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f6_02_w01367

8884

Ccbl2; Kat3; Ccbl2; Kat3; Q71RI9-1; Q71RI9; Q71RI9-2; Q71RI9

59.61

ITMS; CID; 1

_ W

y₁₄²⁺

b₂

T S S

y₁₁²⁺

b₅

D

y₁₀²⁺

W

y₉

T

y₈²⁺

F

y₇²⁺

b₉

D

y₆

P

adpr

R E

y₃

L E S K _

b₂288.1

AMP348.1

y₃363.2

ADP428.0

b₅-H₂O559.2

b₅577.2

y₇²⁺700.3

y₈²⁺757.8

y₁₀²⁺881.8

y₁₁²⁺974.9

y₁₄²⁺1119.4

b₉1126.4

y₆

1302.5

y₉-AMP1314.6

y₈-NH₃1497.5

1195.6

1186.4


1089.6MH₂²⁺-AMP

830.71

MH₃3⁺-2H₂O

400 600 800 1000 1200 1400 1600 1800 2000

m/z (full)

020

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8010

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400600

800

Intensity

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m/z (zoom)

MH₂²⁺-adenine


MH₂²⁺-adenine-2H₂O1177.1


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f5_01_w01050

3664

Chchd2; mCG_130675; Scand3; Q9D1L0; B2RPU8; Q8CEW7

117.29

ITMS; CID; 1

_

adpr

R

y₂₂²⁺

b₂

A P A A

y₁₈

b₆

Q

y₁₇

b₇²⁺

P

y₁₆²⁺

b₈

P

b₉²⁺

A

y₁₄

b₁₀²⁺

A

b₁₁²⁺

A

y₁₂

b₁₂

A P S

y₉

A

y₈

b₁₆

V

y₇

b₁₇

G

y₆

b₁₈²⁺

S P A

b₂₁²⁺

A A P R _

AMP348.1

ADP428.0 y₁₂²⁺

540.8

y₆582.3

b₇²⁺617.2

b₈²⁺665.5

y₇669.4

y₁₆²⁺682.9

b₉²⁺701.3

y₈726.4

y₁₇²⁺731.4

b₁₀²⁺736.8

b₂769.2

b₁₁²⁺772.3

y₁₈²⁺779.9

b₁₂²⁺807.8

y₉825.5

y₂₂²⁺963.5

b₁₆²⁺984.9

b₁₈²⁺1056.9

y₁₂1080.6

b₆1136.4

b₂₁²⁺1176.5

y₁₄1222.654

y₁₇1461.8

y₁₈1558.8

b₁₂1614.6

b₁₆-AMP1621.8

b₁₇-AMP1678.8

1280.2


MH₂²⁺-adenine

1174.0

MH₂²⁺-AMP

1479.2

b₁₂-adenine

400 600 800 1000 1200 1400 1600

m/z (full)

020

4060

8010

012

0

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0200

400600

Intensity

400 600 800 1000 1200 1400 1600

m/z (zoom)


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liverf7_02_w01368

4645

Clec4f; Clecsf13; Kclr; P70194

101.6

ITMS; CID; 1

_ S

y₁₂²⁺

N

y₁₁²⁺

b₃

V

y₁₀²⁺

b₄

Q

y₉²⁺

b₅

M

y₈²⁺

b₆

L

y₇²⁺

b₇

Q

y₆²⁺

S N L

y₃-NH₃

Q

adpr

R A K _

b₃-NH₃284.1

b₃301.2

AMP348.1

ADP428.0

b₄429.2

b₅-NH₃543.2

b₅560.2

y₆²⁺635.8

b₆673.3

y₇²⁺679.3

y₈²⁺743.3

y₉²⁺799.8

b₇801.4

y₁₀²⁺865.4

y₃-NH₃898.3

y₁₁²⁺929.4

y₁₂²⁺978.9

1012.3

MH₂²⁺-adenine

1003.1




955.0

920.8

y₁₁²⁺-NH₃

911.8y₁₁²⁺-NH₃-H2O

906.1

MH₂²⁺-AMP

020

4060

8010

012

0

Rel

ativ

e Ab

unda

nce

0500

10001500

20002500

30003500

Intensity

m/z (zoom)


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f8_02_w01369

2831

Cnn3; Q9DAW9; Q99JN5

70.09

ITMS; CID; 1

_ G A

y₁₁²⁺

S

y₁₀²⁺

b₄-H₂O

Q

y₉²⁺

b₅

A

y₈²⁺

G

y₇²⁺

M

y₆²⁺

b₈

L

b₉

A P G

y₂

T

adpr

R R _

b₄-H₂O326.1

AMP

348.1 b₅-H₂O397.2

b₅-NH₃398.2

b₅415.2

ADP428.0

y₆²⁺599.7

y₇²⁺656.3

b₈716.3

y₈²⁺

721.8

y₉²⁺750.3

y₁₀²⁺785.8

b₉787.4

y₁₁²⁺849.8

y₂

872.3

880.7


595.9

y₁₂3⁺

589.8

y₁₂3⁺-H₂O

567.0

y₁₁3⁺

y₁₁3⁺-H₂O561.1

020

4060

8010

012

0

Rel

ativ

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unda

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0200

400600

800

Intensity

300 350 400 450 500 550 600 650 700 750 800 850 900

m/z (zoom)



Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f4_01_w01049

6818

Cps1; Q8C196; Q561M8

96.06

ITMS; CID; 1

_ G

y₁₀

N D

y₈

b₄

V

y₇

b₅

L

y₆

b₆

V

y₅

b₇

I

adpr

E

y₃

C

y₂-NH₃

N L R _

y₂-NH₃271.2

348.1

b₄386.2

y₃402.2

428.0

b₅-H₂O481.2

b₅499.3

b₆-NH₃581.3

b₆598.3

y₆²⁺673.2

b₇-NH₃694.4

b₇711.4

y₇²⁺722.8

y₈²⁺779.3 y₅-AMP

885.3

y₁₀²⁺886.3

y₆-AMP998.4

y₇-AMP1097.5

y₈-AMP1210.6

y₁₀-AMP1424.6

701.5

MH₂²⁺-ADP ribose

798.3

MH₂²⁺-AMP

903.5

y₇-ADP ribose

1114.5

b₁₀-ADP ribose

617.0

y₅²⁺

200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400

m/z (full)

020

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0

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0200

400600

8001000

1200

Intensity

200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400

m/z (zoom)

AMP ADP


Source:

Scannumber:

Protein:

Peptide Score:

Method:

08146

3014

Croc1; Ube2v1; RP23-118A2.7-001; Q9CZY3-1; Q9CZY3; Q569Y6

110.57

ITMS; CID; 1

_ V N

y₂₀²⁺

M

y₁₉²⁺

S

b₅

G

y₁₇²⁺

b₆

V

y₁₆²⁺

b₇

S

y₁₅²⁺

S

y₁₄²⁺

S

y₁₃²⁺

N G

y₁₁

V

y₁₀²⁺

b₁₃

V

y₉

b₁₄

D

b₁₅²⁺

P

adpr

R

y₆-NH₃

A T A

y₃

V L A K _

y₃331.2

AMP

348.1

ADP428.0

b₅489.2

b₆-H₂O570.3

b₆-NH₃571.3

y₆-NH₃585.4

b₆588.3

b₇-H₂O657.3

b₇-NH₃658.3

b₁₄²⁺667.3

b₇675.3

b₁₅²⁺715.8

y₉²⁺734.3

y₁₀²⁺791.8

y₁₁²⁺841.3

y₁₃²⁺919.4

y₁₄²⁺976.4

y₁₅²⁺1019.9

y₁₆²⁺1063.5

y₁₇²⁺1107.0

y₉-AMP1120.6

y₁₉²⁺1185.0

b₁₃1218.6

y₂₀²⁺1228.5

b₁₄-H₂O1315.6

b₁₄1333.6

y₁₁-AMP1334.7

y₉1467.6

725.6y₉²⁺-H2O

922.3MH₃3⁺-2H₂O

863.1

y₂₁3⁺

400 600 800 1000 1200 1400 1600 1800 2000

m/z (full)

020

4060

8010

012

0

Rel

ativ

e Ab

unda

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0100

200300

400

Intensity

300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500

m/z (zoom)



Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f8_01_w01053

2326

Crsbp1; Lyve1; Xlkd1; Q8BHC0

84.21

ITMS; CID; 1

_

y₈²⁺

I

y₇²⁺

b₂

F

y₆²⁺

b₃

S

y₅

b₄-H₂O

N P

y₃

b₆

adpr

R C G K _

a₂233.2

b₂261.2

b₃-H₂O330.2

b₃348.2

y₃364.2

ADP428.0

b₄-H₂O444.2

y₆²⁺636.7

y₇²⁺680.2

y₈²⁺753.8

y₅-AMP811.3

b₆-AMP909.4

b₆-NH₃1239.4

742.7

733.9

MH₂²⁺-adenine

724.9

685.6


y₇²⁺-adenine612.9

604.0

y₇²⁺-adenine-H₂O

1023.4

y₅-adenine

200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400

m/z (full)

020

4060

8010

012

0

Rel

ativ

e Ab

unda

nce

02000

40006000

8000

Intensity

200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400

m/z (zoom)




Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f9_01_w01054

7029

Egfr; mCG_140518; RP23-295E4.1-001; Q01279; Q3TQS6; Q5SVE8; Q9EP98

76.15

ITMS; CID; 1

_ L L Q E

y₁₉²⁺

adpr

R

y₁₈²⁺

b₆-NH₃

E

y₁₇²⁺

b₇²⁺

L

y₁₆²⁺

V

y₁₅²⁺

b₉*

E P

y₁₃²⁺

L

y₁₂

b₁₂-H₂O

T

y₁₁

P S

y₉

G

y₈²⁺

E A P N

b₂₀²⁺

Q A H L R _

AMP348.1

y₈²⁺453.7

y₁₂²⁺638.8

y₁₃²⁺689.3 b₇²⁺

712.3

y₁₅²⁺794.4

y₁₆²⁺858.9

y₁₇²⁺908.5

y₁₈²⁺965.0

y₁₉²⁺1029.5

y₉1035.5

y₁₁1179.6

y₁₂1276.6

b₆-NH₃1293.5

b₉-AMP1304.6 b₂₀²⁺

1372.1

b₁₂-H₂O1944.8

785.6

y₁₅²⁺-H2O

849.9

y₁₆²⁺-H2O996.8MH₂3⁺-adenosine-H₂O

14021.3b₉-adenosine-H₂O

400 600 800 1000 1200 1400 1600 1800

m/z (full)

020

4060

8010

012

0

Rel

ativ

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unda

nce

050

100150

200250

300

Intensity

400 600 800 1000 1200 1400 1600 1800

m/z (zoom)


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f3_01_w01048

8635

Ehd2; Ehd3; Q9QXY6; Q8R0V6

70.06

ITMS; CID; 1

ac

_

y₁₀²⁺

M

y₉²⁺

F

y₈

b₃

S

y₇²⁺

b₄

W

y₆²⁺

b₅

L G N

b₈²⁺

D D

adpr

R R _

AMP348.1

b₃-H₂O390.1

b₃408.1

b₈²⁺497.2

b₄-H₂O576.2

b₄594.2

y₆²⁺637.2

y₇²⁺693.7

b₅707.3

y₈²⁺786.8

y₉²⁺830.3

y₁₀²⁺903.8

y₈-AMP1225.5

922.8

MH₂²⁺-adenine

913.9

904.6



807.9

816.9

894.8

200 300 400 500 600 700 800 900 1000 1100 1200 1300

m/z (full)

020

4060

8010

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0

Rel

ativ

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020

4060

80100

120

Intensity

200 300 400 500 600 700 800 900 1000 1100 1200 1300

m/z (zoom)

MH₂²⁺-AMP


y₁₀²⁺-H₂O

603.07y₁₀3⁺


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f2_02_w01363

7990

Eif3j; Eif3s1; Eif3j; Eif3s1; ENSMUSG00000043424; mCG_11598; RP23-34E24.2-001; Q66JS6; Q2YDW1; Q3UGC7; Q66JW9; Q99JK5

93.58

ITMS; CID; 1

ac

_ A A A A

b₅

A

y₂₃²⁺

A

y₂₂²⁺

b₇

A

y₂₁²⁺

b₈

A

y₂₀²⁺

b₉

A

y₁₉²⁺

b₁₀

A

y₁₈²⁺

b₁₁

G

b₁₂²⁺

D

y₁₆²⁺

b₁₃

S D

y₁₄²⁺

b₁₅

S

y₁₃²⁺

W

y₁₂²⁺

D

b₁₈

A

y₁₀²⁺

D

y₉

b₂₀²⁺

T F S M E

y₄

D P V

adpr

R K _

b₅398.2

b₁₂²⁺463.2

b₇540.3

b₈611.3

b₉682.4

b₁₀753.4

b₁₁810.4

y₁₀²⁺875.8

b₂₀²⁺901.9

y₁₂²⁺968.9

b₁₃1012.5

y₁₃²⁺1026.4

y₄1040.4

y₁₄²⁺1119.4

b₁₅1214.5

y₁₆²⁺1220.4

y₁₈²⁺1321.5

y₁₉²⁺1350.0

y₂₀²⁺1385.5

y₂₁²⁺1421.0

y₂₂²⁺1456.5

y₂₃²⁺1492.1

b₁₈1586.7

y₉1649.6

1340.7

y₁₉²⁺-H2O

400 600 800 1000 1200 1400 1600 1800

m/z (full)

020

4060

8010

012

0

Rel

ativ

e Ab

unda

nce

010

2030

4050

6070

Intensity

400 600 800 1000 1200 1400 1600 1800

m/z (zoom)

MH₂²⁺-adenine1658.9

1552.7

MH₂²⁺-AMP


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f3_01_w01048

2362

Eif4ebp1; Q60876; Q3TII9

46.16

ITMS; CID; 1

ac

_ S A G

y₁₃²⁺

S S

y₁₁²⁺

C

b₇-H₂O

S

y₉²⁺

Q

y₈²⁺

b₉

T P

y₆²⁺

S

adpr

R

y₄

A

y₃

b₁₄

I P T R _

y₁₆3⁺

AMP348.1

y₃373.2

ADP428.0

y₄486.3

y₆²⁺627.8

b₇-H₂O661.2

y₈²⁺719.8

y₉²⁺770.3

y₁₁²⁺877.9

b₉-H₂O890.3

b₉908.3

b₁₄²⁺987.4

y₁₃²⁺1001.4

b₁₄-AMP1626.6

1106.0

1097.0

MH₂²⁺-adenine


1088.0


1049.1

1040.0

1000.1



991.0

MH₂²⁺-AMP


740.1

733.8

y₁₆3⁺

y₁₆3⁺-H₂O

020

4060

8010

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400600

8001000

Intensity

m/z (zoom)


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f8_01_w01053

2330

Eif4h; Wbscr1; mCG_16718; Eif4h; Wbscr1; mCG_16718; mKIAA0038; Q9WUK2-1; Q9WUK2; Q3V244; Q564E5; Q9WUK2-2; Q9WUK2; Q3TG58; Q80U88; Q9ET00

90.11

ITMS; CID; 1

_

y₁₁²⁺

A

y₁₀

b₂

Y

y₉²⁺

b₃

S

y₈²⁺

b₄

S

y₇²⁺

F

b₆-H₂O

G G G

adpr

R G S R _

a₂207.1

b₂235.1


b₃-H₂O304.1

b₃322.1

AMP348.1

b₄409.2

ADP428.0

y₇²⁺594.2

b₆-H₂O595.3

y₈²⁺667.7

y₉²⁺711.3

y₁₀²⁺754.8

y₁₁²⁺836.3

y₁₀-AMP1161.5

804.2

795.5

MH₂²⁺-adenine

786.3

738.3

698.2

689.27

503.8y₁₀3⁺

y₁₀3⁺-H2O497.6

200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500

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200300

400500

600

Intensity

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m/z (zoom)





MH₂²⁺-AMP


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f6_01_w01051

7505

Eif4h; Wbscr1; mCG_16718; Eif4h; Wbscr1; mCG_16718; mKIAA0038; Q9WUK2-1; Q9WUK2; Q3V244; Q564E5; Q9WUK2-2; Q9WUK2; Q3TG58; Q80U88; Q9ET00

38.74

ITMS; CID; 1

_ T

y₂₁²⁺

V

y₂₀²⁺

A

y₁₉²⁺

b₄

T P

b₆-H₂O

L N

y₁₅²⁺

Q

y₁₄²⁺

b₉-NH₃

V

y₁₃²⁺

b₁₀-H₂O

A

y₁₂²⁺

b₁₁

N

y₁₁-NH₃

P N S

y₈

A

y₇²⁺

b₁₆

I F

b₁₈²⁺

G G

b₂₀

A

adpr

R P R _

b₄

373.2

b₆-H₂O565.3

y₇²⁺651.2

b₁₆²⁺796.4

y₁₂²⁺892.4

b₁₈²⁺898.5

b₉-NH₃907.5

y₁₃²⁺949.4

b₁₀-H₂O977.5

y₁₄²⁺984.9

y₁₅²⁺1034.4

b₁₁1109.6

y₁₉²⁺1260.6

y₂₀²⁺1311.1

y₂₁²⁺1346.6

y₈1414.6

b₁₆1591.8

y₁₁-NH₃1669.6

b₂₀1924.0

840.7

y₁₉3⁺

834.9

y₁₉3⁺-H₂O 874.5y₂₀3⁺

1193.3y₁₉²⁺-adenine

1184.6y₁₉²⁺-adenine-H₂O

789.8y₁₉3⁺-adenine-H₂O

400 600 800 1000 1200 1400 1600 1800

m/z (full)

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8010

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m/z (zoom)

1379.2

1369.8

1361.1

MH₂²⁺-adenine



1322.2


1273.1

MH₂²⁺-AMP


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f2_01_w01047

7609

Eif5a; Ptms; P63242; Q9D0J8; Q66JR8; Q80WS9

80.16

ITMS; CID; 1

ac

_ A D D

y₂₂²⁺

b₄

L

y₂₁²⁺

b₅

D

y₂₀²⁺

b₆

F

y₁₉²⁺

b₇

E

y₁₈²⁺

T

y₁₇²⁺

G D

y₁₅²⁺

A G

y₁₃²⁺

b₁₃-H₂O

A

y₁₂

S

b₁₅-H₂O

A

y₁₀²⁺

T

y₉²⁺

F P

y₇²⁺

M Q C S A L

adpr

R K _

AMP348.1

ADP428.0

b₄457.2

b₅572.2

y₇²⁺702.3 b₆

719.3

y₉²⁺816.3

b₇848.3

y₁₀²⁺889.8

y₁₂²⁺975.9

y₁₃²⁺1019.4

y₁₅²⁺1083.4

y₁₇²⁺1176.5

y₁₈²⁺1205.0

y₁₉²⁺1255.497

b₁₃-H₂O1302.5

y₂₀²⁺1320.0

y₂₁²⁺1393.5 y₂₂²⁺

1451.066

b₁₅-H₂O1460.6

y₁₂-AMP1603.7


1602.7



1505.8MH₂²⁺-AMP

1496.7


1408.9

MH₂²⁺-ADPMH₃3⁺-2H₂O1108.0

400 600 800 1000 1200 1400 1600 1800

m/z (full)

020

4060

8010

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020

4060

80

Intensity

400 600 800 1000 1200 1400 1600 1800

m/z (zoom)


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f7_01_w01052

3620

Erp; Erp60; Grp58; Pdia3; P27773

97.73

ITMS; CID; 1

_

adpr

R

y₁₀²⁺

L

y₉

b₃

A P

y₇

b₅

E Y

y₅

b₇²⁺

E

y₄

A

y₃

b₉²⁺

A

y₂

A T R _


y₂276.2

y₃347.2

AMP348.1

y₄418.2

ADP

428.0

y₅489.3

y₉²⁺504.2

y₁₀²⁺539.8

b₇²⁺700.7

b₉²⁺771.8

y₇781.38

b₃882.3

y₉1007.5

b₅1108.4

b₇-NH₃1383.45

877.4

868.2

MH₂²⁺-adenine


MH₂²⁺-AMP771.2



820.5

811.8

300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500

m/z (full)

020

4060

8010

012

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0200

400600

800

Intensity

300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500

m/z (zoom)


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f3_02_w01364

5838

Errfi1; Mig6; mCG_1312; RP23-272N19.4-001; Q99JZ7; Q3TJN8; Q3TK48

107.09

ITMS; CID; 1

ac

_ S

y₁₃²⁺

T

y₁₂²⁺

b₃-H₂O

A

y₁₁²⁺

b₄

G

y₁₀²⁺

b₅

V

y₉²⁺

b₆

A

y₈²⁺

b₇

A

y₇²⁺

Q

b₉²⁺

D

y₅-NH₃

I

b₁₁²⁺

adpr

R

y₃

b₁₂²⁺

V P

b₁₄²⁺

L K _

b₃-H₂O284.1

AMP348.1

y₃357.2

b₄359.2

b₉²⁺422.2

ADP428.0

b₅-H₂O440.2

b₅458.2

b₆529.3

b₇600.3

y₇²⁺691.3

y₈²⁺755.3

y₉²⁺790.8

y₁₀²⁺826.4

b₁₁²⁺827.3

y₁₁²⁺875.9

b₁₂²⁺876.9

y₁₂²⁺904.4

y₁₃²⁺939.9

b₁₄²⁺981.9

y₅-NH₃1136.5



MH₂²⁺-adenosine-H₂O930.8

MH₂²⁺-AMP881.4

300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600

m/z (full)

020

4060

8010

012

0

Rel

ativ

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unda

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0100

200300

400500

600700

Intensity

300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600

m/z (zoom)


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f7_01_w01052

7725

Es31; Es31; Q8VCU1-1; Q8VCU1; Q8VCU1-2; Q8VCU1; Q63880-1; Q63880; Q63880-2; Q63880

66.26

ITMS; CID; 1

_ L

y₉

b₂

Q

y₈²⁺

b₃

F

y₇²⁺

W

y₆²⁺

T E T

y₃

b₈-H₂O

L

b₉-H₂O

P

adpr

R K _

b₂-NH₃225.1

b₂242.1

AMP348.1

b₃389.2

ADP

428.0y₃-AMP

594.3

y₆²⁺642.8

y₇²⁺693.3

y₈²⁺786.3

y₉²⁺859.9

b₈-H₂O1001.5

b₈-NH₃1002.5

b₉-H₂O1098.6 y₉-AMP

1371.6912.7

903.7

895.0

850.8y₉²⁺-H₂O

792.3y₉²⁺-adenine

1583.21469.8

200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600

m/z (full)

020

4060

8010

012

0

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0500

10001500

20002500

3000

Intensity

200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600

m/z (zoom)

855.9

MH₂²⁺-adenine


MH₂²⁺-adenine-2H₂OMH₂²⁺-adenosine-H₂O

y₉-adeniney₉-adenosine-H₂O


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f6_01_w01051

3868

F11r; Jam1; Jcam; Jcam1; F11r; O88792; Q8VC39

76.07

ITMS; CID; 1

_

y₁₄²⁺

V

y₁₃²⁺

I

y₁₂²⁺

Y

y₁₁²⁺

b₄

S

y₁₀²⁺

b₅

Q P

y₈

S T

y₆²⁺

adpr

R S

y₄

b₁₁²⁺

E G

y₂

E F K _

y₂294.2

AMP348.1

y₆²⁺348.7

b₄463.3

y₄

480.2 b₅-NH₃574.3

b₅591.3

y₆-NH₃679.3

y₈²⁺747.8

y₁₀²⁺839.8

b₁₁²⁺895.3

y₁₁²⁺903.8

y₁₂²⁺947.4

y₁₃²⁺1028.9

y₁₄²⁺1085.4

y₈1494.5


1010.1


961.5

652.6

300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700

m/z (full)

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200300

400500

600

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m/z (zoom)


MH₂²⁺-AMP


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liverf7_02_w01368

2041

Fam105b; Q3UCV8

76.14

ITMS; CID; 1

_ V

y₁₀*

b₂

T

y₉²⁺

b₃-H₂O

A

y₈

b₄

G

y₇²⁺

A

y₆²⁺

b₆-H₂O

Q P

y₄

b₈²⁺

adpr

R

b₉²⁺

A A T R _

b₂201.1


b₃-H₂O254.1

b₄329.2

AMP348.1

y₄418.2

ADP428.0

b₆-H₂O510.3

y₆²⁺606.7

b₈²⁺661.7

y₇²⁺670.8

b₉²⁺697.3 y₉²⁺

734.8

y₁₀²⁺770.3

y₁₀-AMP1192.5

b₉-NH₃1376.5

y₈1411.6

802.6

793.9

784.9745.7MH₂²⁺-adenosine-H₂O

687.8MH₂²⁺-AMP-H₂O

547.4

y₁₁3⁺

514.0

y₁₀3⁺

200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400

m/z (full)

020

4060

8010

012

0

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0200

400600

8001000

12001400

1600

Intensity

200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400

m/z (zoom)

MH₂²⁺-adenine




Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f6_01_w01051

3771

Fam63a; Ni; mCG_140520; Fam63a; Ni; Q76LS9-1; Q76LS9; B7ZMR0; Q6IS37; Q76LS9-2; Q76LS9; Q91VW2

63.68

ITMS; CID; 1

_ V E T A E

y₁₂²⁺

b₆

V

y₁₁²⁺

C

b₈-H₂O

S

y₉

b₉

adpr

R P

y₇

Q

y₆

E

y₅

b₁₃²⁺

L P Q S P R _

AMP348.1

ADP428.0

y₉²⁺526.3

y₅584.3

b₆629.3

y₆-NH₃680.4

y₆697.4

y₇826.4

b₈-H₂O858.4

y₁₁²⁺918.4

y₁₂²⁺998.4

b₁₃²⁺1020.9

y₉1051.6 b₉-NH₃

1556.5

b₉1573.5

1245.1

1235.8

1227.1

MH₂²⁺-adenine




1139.1MH₂²⁺-AMP

MH₂²⁺-AMP-2H₂O

1129.8


1041.8


1012.2b₁₃²⁺-H₂O

MH₂3⁺-2H₂O863.6

300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700 1800 1900

m/z (full)

020

4060

8010

012

0

Rel

ativ

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unda

nce

020

4060

80100

120140

160180

Intensity

300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700 1800 1900

m/z (zoom)


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f6_01_w01051

5611

Fkbp1; Fkbp1a; mCG_13187; RP23-419G21.5-001; FKBP12-Exip3; Fkbp1a; FKBP12-T1; Fkbp1a; FKBP12-T2; Fkbp1a; P26883; A2AT05; Q3UBA0; Q3ULN5; Q1JUQ6; Q1JUQ8; Q1JUQ7

97.2

ITMS; CID; 1

_ G V

y₁₄²⁺

Q

y₁₃²⁺

b₄

V

y₁₂²⁺

b₅

E

y₁₁²⁺

b₆

T

y₁₀²⁺

b₇

I

y₉²⁺

b₈

S

y₈²⁺

P

y₇

G

y₆

D G

adpr

R

y₃

T F P K _


AMP348.1

y₃-NH₃374.1

b₄384.2

y₃391.2

ADP428.0 b₅-H₂O

495.3

b₅513.3

b₆-H₂O596.3

b₆614.3

y₆²⁺623.7

y₈²⁺709.8

b₇727.4

y₉²⁺758.3

b₈-H₂O796.4

y₁₀²⁺801.8

b₈814.4

y₁₁²⁺858.3

y₆-AMP899.4

y₁₂²⁺908.9

y₁₃²⁺973.4

y₇-AMP1014.3

y₁₄²⁺1022.9

964.3

955.4

1097.6

1088.4

1079.6

991.4


MH₂²⁺-adenine


MH₂²⁺-AMP

y₁₅3⁺725.1

719.2y₁₅3⁺-H2O

020

4060

8010

012

0

Rel

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unda

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0200

400600

8001000

12001400

16001800

2000

Intensity

m/z (zoom)

y₁₃²⁺-H2O

y₁₃²⁺-2H2O


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f5_01_w01050

5815

Gnmt; mCG_2695; RP23-76I16.4-001; Q9QXF8; A9C489; Q5I0T9; Q8C4E3

85.94

ITMS; CID; 1

_ T

y₂₀²⁺

b₂

adpr

R S

b₄-H₂O

L G

y₁₆²⁺

V

y₁₅²⁺

A

y₁₄

A

y₁₃

b₉

E

y₁₂²⁺

b₁₀²⁺

G

y₁₁

b₁₁

L P D

y₈

Q

y₇

Y

y₆

A

y₅

b₁₇²⁺

D G E A A R _

AMP348.1 ADP

428.0

y₅503.3

y₁₁²⁺596.8

y₆618.3

y₁₂²⁺653.3 y₇

689.3

b₉²⁺713.8

b₁₀²⁺742.3

y₁₅²⁺781.9

b₁₁²⁺798.8

b₂799.2

y₁₆²⁺817.4

y₈852.4

b₄-H₂O981.3

y₂₀²⁺995.5

b₁₇²⁺1143.5

y₁₁1192.5

1270.3

y₁₃1362.6

b₉1426.5

y₁₄1491.6

b₁₁1596.6

1327.7

1318.7

MH₂²⁺-adenine

1222.0

MH₂²⁺-AMP

m/z (full)

020

4060

8010

012

0

Rel

ativ

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unda

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0200

400600

8001000

Intensity

m/z (zoom)




Source:

Scannumber:

Protein:

Peptide Score:

Method:

liverf7_02_w01368

7711

Gpx3; P46412

85.38

ITMS; CID; 1

_

y₈

M

y₇²⁺

b₂

D

y₆²⁺

b₃

I

b₄

L

b₅

S Y

b₇

M

adpr

R R _

y₅²⁺

b₂247.1

AMP348.1

b₃360.2

b₇²⁺427.7

b₄473.2

b₅560.3

y₆²⁺683.8

y₇²⁺740.3

y₈²⁺797.8

b₇854.4

y₈-AMP1247.5

795.7




690.0MH₂²⁺-AMP

627.4

y₅²⁺

020

4060

8010

012

0

Rel

ativ

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unda

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0200

400600

8001000

Intensity

m/z (zoom)

MH₂²⁺-adenine


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f5_01_w01050

7569

Gsb; Gsn; mCG_21267; RP23-186B18.2-001; Gsb; Gsn; RP23-186B18.2-004; mCG_21267; P13020-1; P13020; A2AL35; Q3TGJ9; Q3U9Q8; Q3UCW8; P13020-2; P13020; A6PWS5; Q6PAC1

66.87

ITMS; CID; 1

_ M Q

y₁₉²⁺

Y P

adpr

R Q T Q

y₁₃²⁺

b₉²⁺

V S

y₁₁

b₁₁

V

y₁₀

b₁₂

L

y₉

P E

b₁₅

G G

y₅

E

y₄

T P L F K _

AMP348.1

ADP428.0

y₄504.3

y₅605.4 y₁₃²⁺

687.4

b₉²⁺837.3

b₁₁²⁺930.4

y₉977.5

b₁₂²⁺986.9

y₁₀1074.5

y₁₁1187.6

y₁₉²⁺1312.6

b₁₁-AMP1512.7 b₁₂-AMP

1625.7 b₁₅-AMP1908.9

b₁₂1972.8


1447.4



400 600 800 1000 1200 1400 1600 1800 2000

m/z (full)

020

4060

8010

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0200

400600

800

Intensity

400 600 800 1000 1200 1400 1600 1800 2000

m/z (zoom)

1350.2

MH₂²⁺-AMP

MH₂²⁺-AMP-H₂O1341.5

1253.5



Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f5_01_w01050

5833

Hcls1; Hs1; Hcls1; P49710; Q542G0; Q3V192; Q8CA06; Q922I8

102.37

ITMS; CID; 1

_ M S

y₂₀²⁺

adpr

R

y₁₉²⁺

E V

y₁₇²⁺

b₆

Q

y₁₆

b₇

Q P S

y₁₃

b₁₀

M P

b₁₂²⁺

V E

y₉

b₁₄²⁺

E P A

y₆

b₁₇²⁺

A P

y₄-NH₃

A

y₃

Q L P K _

AMP348.1

y₃357.2

ADP428.0

y₄-NH₃468.3

y₆653.4

y₁₃²⁺673.9

y₁₆²⁺831.4

b₁₀²⁺858.3

y₉892.5

y₁₇²⁺895.5

b₁₂²⁺956.4

y₁₉²⁺1009.0

y₂₀²⁺1073.5

b₁₄²⁺1085.4

b₁₇²⁺1205.0

b₆1272.4

y₁₃1346.7

b₇1400.5

y₁₆1661.9

b₁₀-H₂O1697.6

b₁₀-NH₃1698.6

b₁₀1715.6

y₁₆²⁺-NH₃822.9

1357.8MH₂²⁺-AMP



400 600 800 1000 1200 1400 1600 1800

m/z (full)

020

4060

8010

012

0

Rel

ativ

e Ab

unda

nce

050

100150

200250

300350

Intensity

400 600 800 1000 1200 1400 1600 1800

m/z (zoom)


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f2_02_w01363

6776

Hmox1; Hmox1; P14901; Q3U5H8; Q3U5U6; Q3TVV4

99.75

ITMS; CID; 1

ac

_ M E

y₁₅²⁺

adpr

R

y₁₄

b₄

P

y₁₃

Q P

b₇

D

b₈-H₂O

S

y₉

b₉

M P

y₇

Q

y₆

b₁₂

D

y₅

L

y₄

S

y₃

E A L K _

y₃331.2

AMP348.1

ADP428.0

y₄-NH₃443.2

y₄460.3

y₉²⁺500.8

y₅547.3

y₆660.4

y₁₃²⁺715.8

b₇²⁺719.2

y₇775.4

y₁₄²⁺779.9

y₁₅²⁺828.4

b₁₂²⁺998.3

y₉1000.5

b₄1097.3

b₉-AMP1308.5

y₁₃-NH₃1413.7 y₁₃

1430.7

b₇1437.5

b₈-H₂O1506.5

y₁₄1558.7

b₉-H₂O1637.5

b₉-NH₃1638.5

b₁₂-AMP1648.6

b₉1655.5

1260.3

MH₂²⁺-adenine

1251.6

1203.7

1154.6


MH₂²⁺-AMP

y₁₅²⁺-H2O819.3

707.0y₁₃²⁺-H2O

400 600 800 1000 1200 1400 1600 1800 2000

m/z (full)

020

4060

8010

012

0

Rel

ativ

e Ab

unda

nce

050

100150

200250

300350

Intensity

400 600 800 1000 1200 1400 1600 1800 2000

m/z (zoom)




Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f3_02_w01364

5433

Hpd; P49429

118.87

ITMS; CID; 1

_ G

y₁₃²⁺

N

y₁₂²⁺

b₃

L

y₁₁

b₄

T

y₁₀²⁺

b₅-H₂O

D

y₉²⁺

b₆-NH₃

L

y₈²⁺

b₇-NH₃

E P

y₆²⁺

N G

y₄

b₁₁

V

y₃

b₁₂²⁺

adpr

R S G M _


b₃-NH₃268.1

b₃285.2

y₃294.1

348.1

b₄386.2

b₅-H₂O483.2

b₅-NH₃484.2

y₆²⁺574.2

b₆-NH₃597.3

y₈²⁺679.7

b₇-NH₃726.3

y₉²⁺744.3

y₁₀²⁺800.8

y₁₁²⁺858.3

b₁₂²⁺904.4

y₁₂²⁺908.8

y₁₃²⁺965.4

y₄991.3

b₁₁-H₂O1092.5

b₁₁-NH₃1093.5

b₁₁1110.5

y₁₁-AMP1368.5

AMP



877.2

MH₂²⁺-AMP

496.2

y₄²⁺

400 600 800 1000 1200 1400 1600 1800

m/z (full)

020

4060

8010

012

0

Rel

ativ

e Ab

unda

nce

0100

200300

400500

Intensity

400 600 800 1000 1200 1400 1600 1800

m/z (zoom)


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f2_01_w01047

7552

Iah1; Q9DB29

86.47

ITMS; CID; 1

ac

_ S L C E

y₁₁

adpr

R

y₁₀²⁺

b₆²⁺

A

y₉

b₇²⁺

A

y₈

S G S

y₅

b₁₁²⁺

A

y₄

b₁₂²⁺

L

y₃

b₁₃²⁺

L

y₂

b₁₄²⁺

W P R _

y₂272.2

AMP348.1

ADP428.0

y₃458.3

y₁₀²⁺529.3

y₄571.3

b₆²⁺650.7

y₅684.4

b₇²⁺686.2

b₁₁²⁺837.3

b₁₂²⁺893.8

y₈899.5

b₁₃²⁺950.4

y₉986.5

b₁₄²⁺1043.4

y₁₁1128.6

1102.5

1111.4

1093.6

MH₂²⁺-adenine



1005.6

997.1

MH₂²⁺-AMP


941.4

b₁₃²⁺-H₂O

774.5

MH₃3⁺-2H₂O

020

4060

8010

012

0

Rel

ativ

e Ab

unda

nce

020

4060

80

Intensity

m/z (zoom)


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f4_01_w01049

9465

Ifi1; Iigp3; Irgm; Irgm1; Ifi1; Iigp3; Irgm; Irgm1; Ifi1; Irgm; Irgm1; RP23-14F5.1-003; Q60766-1; Q60766; Q60766-2; Q60766; Q5NCB5

56.95

ITMS; CID; 1

ac

_ A

y₂₈²⁺

E

y₂₇²⁺

T H

b₅

Y

b₆

A

b₇-H₂O

P

y₂₂²⁺

b₈

L S S

y₁₉²⁺

b₁₁²⁺

A

y₁₈²⁺

b₁₂

F P F

y₁₅²⁺

V

y₁₄²⁺

T

y₁₃²⁺

S Y Q T G

y₈-NH₃

S S

y₆

adpr

R

y₅

L P E V S R _

AMP348.1

ADP428.0

b₈²⁺463.2

b₁₁²⁺585.8

y₅587.3

b₅644.3

b₁₂²⁺659.3

y₆700.4

b₆715.3

b₇-H₂O794.3

b₈925.4

y₁₃²⁺1010.9

y₁₄²⁺1054.4

y₁₅²⁺1104.9

b₁₁-H₂O1152.5

y₁₈²⁺1276.5

b₁₂1317.6

y₁₉²⁺1350.1

y₈-NH₃1467.6

y₂₂²⁺1472.6

y₂₇²⁺1763.3

y₂₈²⁺1813.8

1245.4

1239.7

1174.7MH₂3⁺-AMP

MH₂3⁺-adenine-H₂O

MH₂3⁺-adenine

851.5y₁₈3⁺

900.6y₁₉3⁺

1095.256

b₂₅3⁺

300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700 1800 1900

m/z (full)

020

4060

8010

012

0

Rel

ativ

e Ab

unda

nce

050

100150

200250

Intensity

300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700 1800 1900

m/z (zoom)


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f6_02_w01367

9927

Itgb1; P09055; Q8BUD1

79.8

ITMS; CID; 1

_ N

y₁₈²⁺

V

b₃

L S

y₁₅²⁺

b₅-H₂O

L

y₁₄²⁺

T

y₁₃²⁺

b₇

D

b₈²⁺

adpr

R G

y₁₀

b₁₀²⁺

E

b₁₁²⁺

F

y₈

F

b₁₃²⁺

N

y₆

E

y₅

b₁₅²⁺

L

y₄

b₁₆²⁺

V G

y₂

Q Q R _

y₂303.2

b₃327.2

AMP348.1

y₄488.3

b₅-H₂O509.3

y₅587.3

y₆700.4

b₈²⁺720.8

b₇743.4

b₁₀²⁺813.8 b₁₁²⁺

887.3

y₈943.5

b₁₃²⁺

1017.903

y₁₃²⁺1060.9

y₁₄²⁺1118.4

b₁₅²⁺1139.0

y₁₅²⁺1169.0

b₁₆²⁺1188.5

y₁₀1237.6

y₁₈²⁺1325.6

1364.7

1355.7

1307.9



MH₂²⁺-adenine

1258.5

1250.0

300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700 1800

m/z (full)

020

4060

8010

012

0

Rel

ativ

e Ab

unda

nce

020

4060

80100

120140

160180

200

Intensity

300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700 1800

m/z (zoom)

MH₂²⁺-AMP-NH₃

MH₂²⁺-AMP


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f2_02_w01363

5645

Lcp1; Pls2; Q61233; Q3TJX0; Q3U6N7; Q3U6Z1; Q3U9M7

105.99

ITMS; CID; 1

ac

_ A

y₁₂

adpr

R G

y₁₀

S

y₉

V

b₆

S

y₇²⁺

b₇²⁺

D

y₆

b₈²⁺

E

b₉²⁺

E

y₄

b₁₀

M

y₃

b₁₁²⁺

M

y₂

b₁₂²⁺

E L R _

y₂288.2

AMP348.1

y₃417.2

ADP428.0

y₇²⁺469.2

y₄

548.3 y₁₀²⁺619.8

b₇²⁺628.7

b₈²⁺693.2

b₉²⁺757.7

y₆808.4

b₁₀²⁺823.3

b₁₁²⁺888.8

b₁₂²⁺953.3

b₆-NH₃1124.3

y₉1139.5

b₆1141.4

y₁₀1238.5

b₁₀-AMP1298.4

y₁₂1382.6


200 400 600 800 1000 1200 1400 1600

m/z (full)

020

4060

8010

012

0

Rel

ativ

e Ab

unda

nce

020

4060

80100

120140

160180

Intensity

200 400 600 800 1000 1200 1400 1600

m/z (zoom)

1029.5

1020.5



1011.2


972.4

923.5

MH₂²⁺-AMP

MH₂²⁺-AMP-H₂O914.2

826.3

MH₂²⁺-adenine


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f6_02_w01367

10095

Mcm2; Serpina3k; Spi2; P07759

96.49

ITMS; CID; 1

_ A

y₂₂²⁺

V

y₂₁²⁺

L

y₂₀²⁺

b₄

D

y₁₉²⁺

b₅

V

y₁₈²⁺

b₆

A

y₁₇²⁺

b₇

E

y₁₆²⁺

b₈

T

y₁₅²⁺

G T

y₁₃²⁺

E A A A

y₉²⁺

A T G

b₁₈²⁺

V I

b₂₀²⁺

G G I

b₂₃²⁺

adpr

R K _

AMP348.1

b₄399.2

ADP

428.0

b₅498.3

b₆569.3

b₇698.4

y₉²⁺721.3

b₈799.4

b₁₈²⁺814.4

y₁₃²⁺

863.3897

b₂₀²⁺899.5

y₁₅²⁺978.4

y₁₆²⁺1006.9

y₁₇²⁺1057.5

y₁₈²⁺1122.0

y₁₉²⁺1157.5

y₂₀²⁺1207.0

y₂₁²⁺1264.6

1281.6

y₂₂²⁺1321.1

b₂₃²⁺1333.1

1148.6y₁₉²⁺-H₂O

1112.9y₁₈²⁺-H₂O

1048.2y₁₇²⁺-H₂O

y₂₀²⁺-NH₃

1198.6

y₁₉²⁺-AMP983.6 1232.7

MH₂²⁺-AMP


300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600

m/z (full)

020

4060

8010

012

0

Rel

ativ

e Ab

unda

nce

050

100150

200

Intensity

300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600

m/z (zoom)


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f5_01_w01050

7497

Mcm2; Serpina3k; Spi2; P07759

94.38

ITMS; CID; 1

_ A

y₂₂²⁺

V

y₂₁²⁺

L

y₂₀²⁺

b₄

D

y₁₉²⁺

b₅

V

b₆

A

y₁₇²⁺

b₇

E

y₁₆²⁺

b₈

T G T E A A A A T G

b₁₈²⁺

V I G G I

ri

R K _

y₁₈²⁺

b₄399.2

b₅498.3

b₆-H₂O551.3

b₆569.3 b₇-H₂O

680.3

b₇698.4

b₈799.4

b₁₈²⁺

y₁₆²⁺842.4

y₁₇²⁺892.9

957.5

y₁₉²⁺993.0

y₂₀²⁺1042.5

y₂₁²⁺1100.0

y₂₂²⁺1156.6

814.4

833.9y₁₆²⁺-H₂O

y₂₂3⁺

y₂₂3⁺-H₂O

y₁₈²⁺

948.3

y₁₈²⁺-H₂O

1090.9y₂₁²⁺-H₂O

1147.6y₂₂²⁺-H₂O

984.1y₁₉²⁺-H₂O

400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600

m/z (full)

020

4060

8010

012

0

Rel

ativ

e Ab

unda

nce

02000

40006000

Intensity

400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600

m/z (zoom)

771.4

765.8


Source:

Scannumber:

Protein:

Peptide Score:

Method:

o05357

4999

Mg53; Trim72; Q1XH17

107.74

ITMS; CID; 1

_

adpr

R E

y₁₁

L

y₁₀

S

y₉

S

y₈

b₆²⁺

L

y₇

b₇

N

y₆

S

y₅

Y

y₄

b₁₀

L

y₃

b₁₁

E

y₂

Q L R _


y₂288.2

AMP348.1

y₃416.3

ADP428.0 y₄

545.3

b₆²⁺614.2

y₅658.4

b₇²⁺671.2

y₆821.5

b₁₀²⁺852.8

y₇908.5

b₁₁²⁺917.4

y₈1022.5

y₉1135.6

b₆-H₂O1209.4

y₁₀1222.6

y₁₁1309.7

b₇-H₂O1323.5

b₇1341.5

b₁₀-AMP1357.6

b₁₁-AMP1486.6

020

4060

8010

012

0

Rel

ativ

e Ab

unda

nce

0200

400600

8001000

12001400

1600

Intensity

m/z (zoom)

1057.8

1048.5

1039.5

MH₂²⁺-adenine




951.7MH₂²⁺-AMP

942.4MH₂²⁺-AMP-H₂O

738.6MH₂²⁺-2H₂O

732.2MH₂²⁺-3H₂O


Source:

Scannumber:

Protein:

Peptide Score:

Method:

o05396

4998


108.56

ITMS; CID; 1

_

ri

R E L

b₄-H₂O

S

b₅-H₂O

S

y₈

b₆²⁺

L

y₇

b₇²⁺

N

y₆

b₈

S

y₅

b₉

Y

y₄

b₁₀²⁺

L

y₃

b₁₁²⁺

E

y₂

b₁₂²⁺

Q

b₁₃²⁺

L R _

y₂288.2

y₃416.3

b₆²⁺449.7

y₇²⁺454.7

b₇²⁺506.7

y₄545.3

b₈²⁺550.2

b₉²⁺631.4 y₅

658.4

b₄-H₂O680.3

b₁₀²⁺688.3

b₁₁²⁺752.8

b₅-H₂O767.3

b₁₂²⁺816.9 y₆

821.5

b₁₃²⁺873.4

y₇908.5

y₈1022.5 b₈

1099.5 b₉1262.5

b₁₃²⁺-H₂O864.4

679.4b₁₀²⁺-H₂O

622.3

b₉²⁺-H₂O

752.9b₁₁²⁺-H₂O

200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400

m/z (full)

020

4060

8010

012

0

Rel

ativ

e Ab

unda

nce

0100

200300

400500

600700

Intensity

200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400

m/z (zoom)


Source:

Scannumber:

Protein:

Peptide Score:

Method:

o05353

6865


55.47

ITMS; CID; 1

_ I L S E

b₅

S

b₆

P

y₁₆²⁺

b₇

P P

y₁₄²⁺

A

adpr

R L D I

y₉²⁺

b₁₄²⁺

Q

y₈

b₁₅

L

y₇

P V

y₅

I

y₄

S D

y₂

D F K _

b₁₆3⁺


y₂294.2

AMP348.1

y₈²⁺460.7

b₅-H₂O512.3

y₉²⁺517.3

y₄524.2

b₅530.3

y₅611.3

b₆627.3

b₇724.4

y₇823.4

y₈920.5

b₁₄²⁺1029.9

b₁₅²⁺1086.5

y₁₄²⁺1100.5

y₁₆²⁺1184.5

b₁₅-AMP1824.9

b₁₅²⁺-H₂O1077.3

1021.3

b₁₄²⁺-H₂O

854.9

y₁₈3⁺

833.7y₁₉3⁺

b₁₄3⁺687.3

725.1b₁₅3⁺

757.0

b₁₆3⁺

200 400 600 800 1000 1200 1400 1600 1800

m/z (full)

020

4060

8010

012

0

Rel

ativ

e Ab

unda

nce

0200

400600

800

Intensity

200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700 1800

m/z (zoom)

y₁₈3⁺y₁₉3⁺


Source:

Scannumber:

Protein:

Peptide Score:

Method:

o05391

6497


91.59

ITMS; CID; 1

_ I

y₂₁²⁺

L S

y₁₉²⁺

E

y₁₈²⁺

b₅

S

y₁₇²⁺

b₆

P

b₇-H₂O

P P

b₉-H₂O

A

b₁₀

ri

R L

y₁₁

b₁₂²⁺

D I

y₉

b₁₄

Q

y₈-NH₃

b₁₅

L P

y₆

V

y₅

I

y₄

S D

y₂

b₂₁²⁺

D F K _

y₂294.2

b₅-H₂O512.3

y₄524.2

b₅530.3

y₅611.3

b₆627.3

b₇-H₂O706.4

y₆724.4

b₁₂²⁺744.9

b₁₄²⁺865.4

b₉-H₂O874.5

y₈-NH₃903.4

y₉1033.6

y₁₇²⁺1068.5

y₁₈²⁺1117.1

y₁₉²⁺1160.6

b₁₀-phosphate1162.6

b₂₁²⁺1235.1

b₁₀1260.6

y₂₁²⁺1268.6

y₁₁1274.7

b₁₄-H₂O1711.8

b₁₄1729.8 b₁₅-H₂O

1824.9

b₁₅1842.9

300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700 1800 1900

m/z (full)

020

4060

8010

012

0

Rel

ativ

e Ab

unda

nce

0100

200300

400500

600

Intensity

300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700 1800 1900

m/z (zoom)


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f7_01_w01052

7328

Mms2; Ube2v2; Uev2; mCG_126823; mCG_127675; RP23-310H3.1-001; mCG_126823; RP23-310H3.1-003; Ube2v2; Q9D2M8-1; Q9D2M8; Q4VBX4; A6X925

67.65

ITMS; CID; 1

_

y₁₅²⁺

W

y₁₄

T

b₃-H₂O

G

y₁₂²⁺

b₄-H₂O

M

y₁₁²⁺

b₅

I

y₁₀

b₆

I

y₉²⁺

b₇

G P P

y₆

adpr

R

y₅

T N Y E N R _


b₃-H₂O327.1

AMP348.1

ADP428.0

b₄-H₂O458.2

b₅-H₂O571.3

b₅589.3

y₅

695.3

b₆702.4

b₇759.4

y₆796.4

y₉²⁺844.3

y₁₀²⁺872.8

y₁₁²⁺929.4

y₁₂²⁺985.9

y₁₄²⁺1079.9

y₁₅²⁺1130.5

y₁₀-AMP1397.6

y₁₄-AMP1811.8

1156.2

1147.1

1138.6

1098.5

1050.2MH₂²⁺-AMP

1041.1MH₂²⁺-AMP-H₂O

m/z (full)

020

4060

8010

012

0

Rel

ativ

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unda

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050

100150

Intensity

m/z (zoom)

MH₂²⁺-adenine





Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f7_01_w01052

3692

Kiaa1248; Ndr2; Ndrg2; Kiaa1248; Ndr2; Ndrg2; Q9QYG0-1; Q9QYG0; Q8CGR9; Q9QYG0-2; Q9QYG0; Q9CTJ5

56.57

ITMS; CID; 1

_ T

y₁₄²⁺

A

y₁₃²⁺

b₃-H₂O

S

y₁₂²⁺

b₄

L

y₁₁²⁺

b₅

T

y₁₀²⁺

S

y₉²⁺

A A S I

b₁₁

D G S

y₂

adpr

R S R _

b₃-H₂O242.1

y₂262.2

AMP348.1

b₄-H₂O355.2

b₄373.2 ADP

428.0

b₅-H₂O456.2

b₅474.3

y₉²⁺745.3

y₁₀²⁺780.8

y₁₁²⁺824.3

y₁₂²⁺874.8

y₁₃²⁺931.4

y₁₄²⁺974.9

b₁₁1018.5

993.4

936.9

MH₂²⁺-adenine


887.6MH₂²⁺-AMP


966.1

y₁₄²⁺-H₂O650.7

y₁₄3⁺-H₂O644.5

020

4060

8010

012

0

Rel

ativ

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01000

20003000

4000

Intensity

m/z (zoom)

789.8

MH₃3⁺-H₂O695.8

y₁₄3⁺


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f6_01_w01051

8375

Nipsnap1; mCG_14685; RP23-64E17.1-001; Nipsnap1; O55125; Q5SVF7; Q3UGS1

69.6

ITMS; CID; 1

_

adpr

R G W D E N

y₈

b₇²⁺

V

y₇

b₈²⁺

Y

y₆

b₉²⁺

Y

y₅

b₁₀²⁺

T

y₄

b₁₁²⁺

V

b₁₂²⁺

P L V R _

AMP348.1

ADP428.0

y₄484.3

y₅583.4

y₆684.4

b₇²⁺699.7

b₈²⁺781.3

y₇847.5

b₉²⁺862.8

b₁₀²⁺913.3

b₁₁²⁺962.9

y₈1010.6

b₁₂²⁺

1011.4

1137.2

MH₂²⁺-adenine

1128.0


1118.8


1079.2

1031.2MH₂²⁺-AMP

954.0

903.9

020

4060

8010

012

0

Rel

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020

4060

80

Intensity

m/z (zoom)

MH₂²⁺-adenosine-H₂Ob₁₁²⁺-H₂O

b₁₀²⁺-H₂O


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f3_02_w01364

8701

Np; Pnp; Pnp1; Pnp; Pnp1; P23492; Q543K9

162.22

ITMS; CID; 1

_ F

y₁₄

b₂

V

b₃

S

y₁₂²⁺

b₄

I

y₁₁²⁺

b₅

L

y₁₀²⁺

b₆

M

y₉²⁺

E

y₈²⁺

b₈

S

y₇

b₉-H₂O

I

y₆²⁺

P

y₅

b₁₁

L P D

adpr

R G S _

b₂247.1

b₃-H₂O316.2

b₃334.2

AMP

348.1

ADP428.0

b₄-H₂O429.2

b₄447.3

b₅-H₂O542.3

b₅560.3

y₆²⁺593.2

y₇²⁺641.7

b₆-H₂O673.4

b₆691.4

y₈²⁺698.3

y₉²⁺741.8

y₁₀²⁺806.3

y₁₁²⁺871.8

b₈907.5

y₁₂²⁺928.4

b₉-H₂O1002.5

y₁₄²⁺1028.4

y₅1072.3

b₁₁-H₂O1212.7

b₁₁

1230.7

y₇1282.4

1708.8

b₉-H₂O

y₁₄²⁺-NH₃

862.73

632.9

y₁₄-AMP

m/z (full)

020

4060

8010

0

Rel

ativ

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unda

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0100

200300

400500

Intensity

m/z (zoom)



1020.0

y₁₄²⁺-H₂O 1019.2

y₁₂²⁺-H₂O 919.5

y₁₁²⁺-H₂O

797.2y₁₀²⁺-H₂O

y₇²⁺-H₂O


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f4_02_w01365

7388

Pah; Pah; P16331; Q3UEH8; Q91WV1

79.09

ITMS; CID; 1

ac

_ A

y₁₁²⁺

A

y₁₀²⁺

b₃

V

y₉²⁺

b₄

V

y₈²⁺

b₅

L

y₇²⁺

E

y₆²⁺

N

b₈

G

b₉-NH₃

V L

b₁₁

S

adpr

R K _

b₃284.2

AMP

348.1

b₄383.2

ADP428.0

b₅496.3

y₆²⁺600.7 y₇²⁺

657.8

y₈²⁺722.3

y₉²⁺778.8

b₈-NH₃779.4

b₈796.4

y₁₀²⁺828.4

y₁₁²⁺877.9

b₉-NH₃878.5

b₁₁1095.6


893.26


020

4060

8010

012

0

Rel

ativ

e Ab

unda

nce

0200

400600

8001000

12001400

16001800

2000

Intensity

m/z (zoom)


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f9_01_w01054

6400

Pbp; Pebp; Pebp1; Pbp1; mCG_7941; P70296; Q3TGC5; Q5EBQ2

104.17

ITMS; CID; 1

_ L

y₁₆²⁺

Y

y₁₅²⁺

b₃-H₂O

T

y₁₄²⁺

b₄

L

y₁₃²⁺

b₅

V

y₁₂²⁺

L

y₁₁²⁺

T

y₁₀

b₈

D P

y₈²⁺

D A P S

adpr

R K

y₂

b₁₆²⁺

D P K _

y₂244.2

AMP348.1

b₃-H₂O360.2

ADP428.0

b₄491.3

b₅-H₂O572.3

b₅

590.3548

y₈²⁺720.3

y₁₀²⁺826.3

y₁₁²⁺883.8

b₈919.5

y₁₂²⁺934.4

y₁₃²⁺990.9

y₁₄²⁺1040.4

y₁₅²⁺1097.0

y₁₆²⁺1147.5

b₁₆²⁺1164.0

y₁₀-AMP1304.6

1218.2

MH₂²⁺-adenine

1209.3b₁₆²⁺-H₂O

1164.0

1112.2


MH₂²⁺-AMP

1103.3

200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600

m/z (full)

020

4060

8010

012

0

Rel

ativ

e Ab

unda

nce

020

4060

80100

120

Intensity

200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600

m/z (zoom)



Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f6_01_w01051

7215

Pdap1; mCG_17262; Q3UHX2; B2RTB0; Q1WWJ8

65.47

ITMS; CID; 1

_ G V

y₁₆²⁺

E G

y₁₄²⁺

b₅

I

y₁₃²⁺

b₆

I

y₁₂²⁺

b₇

D I

y₁₀²⁺

b₉

E

y₉²⁺

N P

b₁₂²⁺

N

mo

R

y₅

b₁₄²⁺

V

b₁₅²⁺

A

b₁₆²⁺

Q T

b₁₈²⁺

T K _

AMP348.1

b₅456.2

y₅548.3

b₆569.3

b₁₂²⁺626.3

b₇684.4

y₉²⁺778.3

y₁₀²⁺835.3

b₉926.5

y₁₂²⁺956.4

y₁₃²⁺1013.9

b₁₄²⁺1024.4

b₁₅²⁺1059.9

y₁₄²⁺1070.5

b₁₆²⁺1124.0

y₁₆²⁺1155.512

b₁₈²⁺1225.0

1230.3

1221.7

1173.6

1212.6

MH₂²⁺-adenine

MH₂²⁺-adenosine-H₂O-NH₃


MH₂²⁺-adenine-NH₃

1115.8

1124.8


MH₂²⁺-AMP

020

4060

8010

012

0

Rel

ativ

e Ab

unda

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0100

200300

400

Intensity

m/z (zoom)


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f6_01_w01051

9281

Pgm2; RP24-156I23.2-002; Pgm2; RP24-156I23.2-001; Pgm1; A2CEK3; Q3U6X6; Q3UGE3; Q5RJV4; Q66JR7; Q7TNU0; Q99JW6; Q9D0F9

130.01

ITMS; CID; 1

_ D

y₁₂²⁺

a₂

L

y₁₁

b₃

E

y₁₀²⁺

b₄

A

y₉²⁺

b₅

L

y₈

b₆

M

y₇²⁺

b₇

L

y₆

D

adpr

R S F

y₂

V G K _

a₂201.1

y₂204.1

AMP348.1

b₃358.2

ADP428.0

b₄429.2

b₅542.3

y₆²⁺

617.7

b₆673.3

y₇²⁺675.2

y₈²⁺731.8

b₇-H₂O768.4

b₇

786.4

y₉²⁺797.3

y₁₀²⁺853.9

y₁₁²⁺889.4

y₁₂²⁺953.9

y₆1234.5

y₁₁1777.7

944.8y₁₂²⁺-H₂O

1000.6

MH₂²⁺-adenine


894.3MH₂²⁺-AMP

200 400 600 800 1000 1200 1400 1600 1800

m/z (full)

020

4060

8010

012

0

Rel

ativ

e Ab

unda

nce

0200

400600

8001000

1200

Intensity

200 400 600 800 1000 1200 1400 1600 1800

m/z (zoom)


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f3_02_w01364

4800

Kiaa1092; Plce2; Plcl2; Q8K394

64.38

ITMS; CID; 1

ac

_ A E C

y₁₉²⁺

G

b₅

adpr

R

b₆

G A

y₁₅

b₈

A

b₉

G

b₁₀²⁺

G

y₁₂

b₁₁

A

y₁₁

b₁₂

L

b₁₃²⁺

P

y₉

T

y₈

S

y₇

b₁₆²⁺

P S P A L G A K _

AMP348.1

y₁₁²⁺513.3

y₁₂²⁺569.8

y₇643.4

b₁₀²⁺735.7

y₈740.4

b₁₁²⁺771.2

y₉827.5

b₁₃²⁺876.3

b₁₆²⁺1018.9

y₁₁1025.6

y₁₉²⁺1110.5

y₁₂-NH₃1121.6

y₁₂1138.6

b₅1157.3

b₆1214.3

b₁₂-AMP1307.5

y₁₅1323.73

b₈1356.4

b₉1413.4

b₁₁1541.5

b₁₂1654.6

1272.6

1263.6

MH₂²⁺-adenine



1166.5MH₂²⁺-AMP

300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700 1800

m/z (full)

020

4060

8010

012

0

Rel

ativ

e Ab

unda

nce

020

4060

80100

120140

160

Intensity

300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700 1800

m/z (zoom)


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f6_01_w01051

8891

mKIAA0106; Prdx6; Aop2; Ltw4; Prdx5; Prdx6; Q6A0D0; Q6GT24; O08709; Q53ZU7

71.59

ITMS; CID; 1

_ P G G L

b₅

L

y₁₇²⁺

b₆

L

y₁₆²⁺

b₇

G

y₁₅²⁺

b₈

D

y₁₄²⁺

b₉

E

y₁₃²⁺

b₁₀

A P

b₁₂

N

y₁₀²⁺

b₁₃²⁺

F

y₉²⁺

E

b₁₅

A N T

b₁₈-NH₃

T I G

adpr

R I R _

b₉²⁺426.7

b₅438.3

b₁₀²⁺

462.2

b₆551.4

b₁₂²⁺567.8

b₇608.4

b₁₃²⁺641.3

b₈

723.4y₉²⁺

771.8

y₁₀²⁺836.3

b₉852.4

b₁₀923.5

y₁₃²⁺1015.4

y₁₄²⁺

1050.9

y₁₅²⁺1115.5

b₁₂-H₂O1116.6

b₁₂

1134.6

y₁₆²⁺1173.0

y₁₇²⁺

1201.5

b₁₅

1481.7b₁₈-NH₃

1780.8

677.5

y₁₃3⁺

671.8y₁₃3⁺-H₂O

1006.7y₁₃²⁺-H₂O


300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700 1800

m/z (full)

020

4060

8010

012

0

Rel

ativ

e Ab

unda

nce

0500

10001500

2000

Intensity

300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700 1800

m/z (zoom)


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f5_01_w01050

7762


73.11

ITMS; CID; 1

_ P

y₂₁²⁺

G G L

b₅

L

y₁₇²⁺

b₆

L

b₇

G

b₈

D

y₁₄

E

y₁₃²⁺

b₁₀

A P

y₁₁

b₁₂-H₂O

N

y₁₀²⁺

F

b₁₄

E

y₈

b₁₅

A

b₁₆

N

y₆²⁺

b₁₇-H₂O

T T

b₁₉

I G

adpr

R I R _

AMP348.1

ADP428.0

b₅438.3

b₆551.4

b₇608.4

y₆²⁺628.8

b₁₄²⁺705.8

b₈723.4

y₁₀²⁺836.3

b₁₀923.5

y₁₃²⁺1015.4

y₁₄²⁺1050.9

b₁₂-H₂O1116.6

y₈-AMP1124.5

y₁₇²⁺1201.5

y₂₁²⁺1399.6

b₁₄1410.7

b₁₅-NH₃1464.7

y₁₁-AMP1471.7

b₁₅1481.7

b₁₆1595.8

b₁₇-H₂O1678.8

y₁₄-AMP

1753.8

b₁₉1910.9

1352.2

1303.3


1294.7


933.9MH₃3⁺-adenine-H₂O

MH₃3⁺-adenine-2H₂O927.9

901.8

MH₃3⁺-adenosine-H₂O

869.3

863.2

MH₃3⁺-AMP-H₂O

MH₃3⁺-AMP

400 600 800 1000 1200 1400 1600 1800

m/z (full)

020

4060

8010

012

0

Rel

ativ

e Ab

unda

nce

02000

40006000

8000

Intensity

400 600 800 1000 1200 1400 1600 1800

m/z (zoom)

MH₂²⁺-AMP


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f6_01_w01051

8853


88.55

ITMS; CID; 1

_ P G

y₂₀²⁺

G

y₁₉²⁺

b₄

L

y₁₈²⁺

b₅

L

y₁₇²⁺

b₆

L

y₁₆²⁺

b₇

G

y₁₅²⁺

b₈

D E

y₁₃²⁺

b₁₀

A P N

b₁₃-NH₃

F

y₉²⁺

b₁₄

E A

y₇

N

y₆²⁺

b₁₇-H₂O

T

y₅

T I G

ri

R I R _

b₄325.2

b₅438.3

y₆²⁺464.2

b₆551.4

y₉²⁺607.3

b₇608.4

b₈723.4 y₅

826.4

y₁₃²⁺850.9

y₆-NH₃910.4

b₁₀923.5

y₁₅²⁺950.9 y₁₆²⁺

1008.5

y₇1028.5

y₁₇²⁺1037.0

y₁₈²⁺1093.5

y₁₉²⁺1150.0 y₂₀²⁺

1206.6

b₁₃-NH₃1264.6

b₁₄-H₂O1392.7

b₁₄1410.7

b₁₇-H₂O1678.8

300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700

m/z (full)

020

4060

8010

012

0

Rel

ativ

e Ab

unda

nce

0100

200300

400500

Intensity

300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700

m/z (zoom)


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f3_02_w01364

5860

Prl2; Ptp4a2; mCG_13525; RP23-114O4.2-001; O70274; A2ACH4; Q3UXF9

92.86

ITMS; CID; 1

ac

_ M N

y₁₂

b₃

adpr

R P

y₁₀

b₅

A P

y₈

b₇²⁺

V

y₇

b₈²⁺

E I

b₁₀²⁺

S

y₄

Y

y₃

E N M R _

AMP348.1

y₃420.2

ADP428.0

y₇²⁺

456.7

y₄549.2

y₁₀²⁺619.3

b₇²⁺675.3

y₁₂²⁺703.3

b₈²⁺739.8

b₁₀²⁺839.8

y₇912.4

b₃985.3

y₈1041.5

b₅

1153.4y₁₀

1237.6y₁₂

1405.7

1128.0

MH₂²⁺-adenine


1070.7


1021.8

MH₂²⁺-AMP

839.8b₁₀²⁺-H₂O

300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700 1800

m/z (full)

020

4060

8010

012

0

Rel

ativ

e Ab

unda

nce

020

4060

80100

120140

160180

Intensity

300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700 1800

m/z (zoom)


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liverf7_02_w01368

2289

Ptma; mCG_133549; Gm12504; mCG_1041267; OTTMUSG00000007209; P26350; Q0VGU2; Q3U6E4; Q3U8X6; Q3UA54; Q8C2T3; Q8CAQ5

102.97

ITMS; CID; 1

_

adpr

R

y₁₂

b₂

V

y₁₁

A

y₁₀

E

y₉

D

b₆

D

y₇

b₇²⁺

E

b₈

D

b₉²⁺

D

y₄

b₁₀²⁺

D V

y₂

b₁₂

D T K _

y₂248.2

AMP348.1

ADP428.0

y₄462.3 y₁₀²⁺

583.7

b₆²⁺614.2

b₇²⁺

678.7

y₁₂²⁺683.8 y₇-NH₃

790.3

b₉²⁺793.7

b₂797.2

y₇807.3

b₁₀²⁺851.3

b₁₂²⁺958.3

y₉1051.4

y₁₀1166.4

b₆

1227.4

y₁₁1295.5

y₁₂

1366.5

b₈1471.4

b₁₂-AMP1568.5


1005.3

957.3

908.5

899.7 947.4b₁₂²⁺-H₂O

781.5b₂-H₂O

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MH₂²⁺-adenosine

MH₂²⁺-AMP



Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f3_02_w01364

6628

Rab11b; mCG_22989; Rab11B; RAB11B; Rab11; Rab11a; mCG_12782; Rab11A; Rab11b; P46638; Q78ZJ8; P62492; P97345; Q0PD45; Q3UZ37; Q9QWU0

77.19

ITMS; CID; 1

ac

_ G T

y₉

b₃-H₂O

adpr

R

y₈

b₄

D

y₇

b₅

D

y₆

E

y₅

Y

y₄

b₈

D

y₃

Y

b₁₀

L F K _

AMP

348.0704

y₃

407.2653

ADP428.0367

y₄

570.3286

y₅

685.3556

b₈²⁺768.2216

b₅-AMP781.24

y₆848.4

b₃-H₂O880.2

y₇977.5

b₄

1013.3

y₈1092.5

b₅

1128.3

b₈-AMP1188.4

y₉1207.5

b₈-H₂O1517.4

b₈1535.4

b₁₀1811.6

985.4

976.46

967.2




928.1

879.3

870.8MH₂²⁺-AMP-H₂O

300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700 1800 1900

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400600

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MH₂²⁺-adenine

MH₂²⁺-AMP


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f3_02_w01364

6575

Rab11b; mCG_22989; Rab11B; RAB11B; Rab11; Rab11a; mCG_12782; Rab11A; Rab11b; P46638; Q78ZJ8; P62492; P97345; Q0PD45; Q3UZ37; Q9QWU0

172.13

ITMS; CID; 1

ac

_ G T

y₉

ri

R

y₈

b₄

D

y₇

b₅

D

y₆

b₆

E

b₇

Y

y₄

b₈

D

y₃

b₉

Y

y₂

b₁₀

I

b₁₁

F K _

y₂294.2

y₃407.3

y₄570.3

b₄-NH₃667.2

b₄684.2

b₅-NH₃782.2

b₅799.3

y₆848.4

b₆-H₂O910.3

b₆928.3

y₇-NH₃960.4

y₇977.5

b₇-H₂O1073.3b₇

1091.4

y₈1092.5

b₈-H₃PO₄1108.4

b₈-H₂O1188.4

b₈-NH₃1189.4

b₈1206.4

y₉1207.5

b₉-H₂O1351.4b₉-NH₃

1352.4

b₉1369.4

b₁₀-NH₃1465.5

b₁₀1482.5

b₁₁-H₂O1611.6

b₁₁1629.6

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MH₂²⁺-H₂O879.5

870.3MH₂²⁺-2H₂O

839.5MH₂²⁺-H₃PO₄


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f6_01_w01051

3653

Scfd1; Stxbp1l2; Scfd1; Stxbp1l2; Scfd1; Stxbp1l2; Q8BRF7-1; Q8BRF7; Q8BRF7-3; Q8BRF7; Q8BRF7-2; Q8BRF7

65.11

ITMS; CID; 1

_ V

y₁₇²⁺

N

y₁₆²⁺

b₃

L

y₁₅²⁺

E

y₁₄²⁺

b₅

E

y₁₃²⁺

b₆-H₂O

S T G

y₁₀²⁺

b₉-NH₃

V E

b₁₁

N

b₁₂

S P

b₁₄

A

y₄

G A

adpr

R P K _

b₃327.2

AMP348.1

ADP

428.0

b₅585.3

b₆-H₂O654.3

y₁₀²⁺784.3

b₉-NH₃912.4

y₁₃²⁺912.9

y₁₄²⁺956.4

y₄1012.4

y₁₅²⁺1020.9

y₁₆²⁺1085.4

y₁₇²⁺1142.0

b₁₁-H₂O1154.5

b₁₁1172.5

b₁₂1259.6

b₁₄1427.7


1074.7

MH₂²⁺-AMP

820.9MH₃3⁺-2H₂O

799.8y₁₇3⁺

894.1y₁₃²⁺-H₂O

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Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f3_01_w01048

4931

Sec14l4; mCG_9615; RP23-81P12.7-001; Q8R0F9; Q5SQ22

89.51

ITMS; CID; 1

ac

_ M

adpr

R

b₃

G

y₁₄

Q

y₁₃

V G

y₁₁²⁺

b₇²⁺

D

y₁₀²⁺

b₈

L

y₉

b₉-H₂O

S

y₈

P

y₇

Q

y₆

b₁₂²⁺

Q

y₅

b₁₃²⁺

Q

y₄

E

y₃

A L A R _

348.1

y₃359.2

y₄430.3

y₉²⁺520.8

y₅559.3

y₁₀²⁺564.3

y₁₁²⁺620.8

b₇²⁺664.2

y₆687.4

y₁₃²⁺706.9

b₈²⁺720.8

y₁₄²⁺756.4

y₇815.4

b₃928.2

b₁₂²⁺940.9

y₈943.5

b₁₃²⁺1004.9

y₉1040.5

1110.6

y₁₃1412.7

b₈1440.5

b₉-H₂O1509.5

b₉-NH₃1510.5

y₁₄1511.8

1101.5

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AMP




MH₂²⁺-AMP

1159.6



Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f3_01_w01048

3794

D5Ertd606e; Sept11; D5Ertd606e; Sept11; D5Ertd606e; Sept11; Q8C1B7-1; Q8C1B7; Q8C1B7-2; Q8C1B7; Q8C1B7-3; Q8C1B7

76.93

ITMS; CID; 1

ac

_ A

y₁₁²⁺

b₂

V

y₁₀²⁺

b₃

A

y₉

b₄

V

y₈²⁺

b₅

G

y₇²⁺

b₆²⁺

adpr

R

y₆

b₇

P

y₅-NH₃

S N E

y₂

E L R _

b₂213.1

b₃284.2

y₂

288.2

AMP348.1

b₄383.2

y₇²⁺422.7

b₅

440.3

b₆²⁺569.2

b₇²⁺617.7

y₅-NH₃643.3

y₆747.4

y₈²⁺771.3

y₉²⁺799.8

y₁₀²⁺849.3

y₁₁²⁺884.9

b₇1234.5

y₉-AMP1251.5

923.4

914.5

MH₂²⁺-adenine

866.5

817.3

590.4

y₁₁3⁺623.4

y₁₂3⁺

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400

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MH₂²⁺-AMP


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f2_01_w01047

7323

Serpina3g; Spi2A; Serpina3f; BC049975; Serpina3f; Q5I2A0; Q3U0Y1; Q4V9W0; B2RT49; Q3UDB3; Q3UE77; Q3UTZ5; Q80X76

105.39

ITMS; CID; 1

ac

_

y₂₁²⁺

A G

y₁₉²⁺

b₃

V

y₁₈²⁺

b₄

S

y₁₇²⁺

P

y₁₆²⁺

A

y₁₅²⁺

b₇

V

y₁₄²⁺

b₈

F

y₁₃²⁺

b₉

G

y₁₂²⁺

C P D V T

y₇²⁺

L G

adpr

R N T A V R _

b₃270.1

b₄-H₂O339.2

AMP348.1

b₄

357.2

ADP

428.0

b₇624.3

y₇²⁺

657.8

b₈771.4

b₉828.4

y₁₂²⁺

920.4

y₁₃²⁺1000.4

y₁₄²⁺1028.9

y₁₅²⁺1102.5

y₁₆²⁺1152.0

y₁₇²⁺1187.5

y₁₈²⁺1236.0

y₆-NH₃1240.4

y₁₉²⁺1279.5

y₂₁²⁺1357.6


1337.7

1289.59

1241.0MH₂²⁺-AMP

824.5

y₁₈3⁺

853.4

y₁₉3⁺

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Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f3_01_w01048

8950

Serpina3g; Spi2A; Q5I2A0; Q3U0Y1; Q4V9W0

78.49

ITMS; CID; 1

_

adpr

R L C F G

y₇

b₆²⁺

D P

y₅

b₈²⁺

L

y₄

b₉²⁺

C

b₁₀²⁺

L

y₂

b₁₁

I

b₁₂

G Q _

y₂204.1

AMP348.1

ADP428.0

y₄430.3

y₅590.3

b₆²⁺645.7

b₈²⁺750.8

y₇

800.4

b₉²⁺830.8

b₁₀²⁺887.3

b₁₁²⁺943.9

b₁₂²⁺972.4

b₁₁-AMP1539.7

b₁₂-AMP1596.7

978.1


MH₂²⁺-adenine

968.9871.7MH₂²⁺-AMP

1402.5

b₁₂-ADP ribose1751.29

b₁₁-adenine

200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700 1800

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020

4060

80100

120

Intensity

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Source:

Scannumber:

Protein:

Peptide Score:

Method:

o05313

4607

Serpinb1; Serpinb1a; Serpinb1c; Q9D154; Q8BK60; Q5SV42

101.42

ITMS; CID; 1

_

y₁₅

A D

y₁₃

b₃

L

y₁₂

b₄

S G

y₁₀²⁺

b₆

M

y₉²⁺

b₇-H₂O

S G S

adpr

R D

b₁₂

L

y₃

b₁₃

F

y₂

I S K _

y₂234.1


b₃

300.2

y₃347.2

AMP348.1

b₄387.2

ADP428.0 b₆-H₂O

557.2

b₆

575.2

b₇-H₂O644.3

y₉²⁺782.3

y₁₀²⁺825.8

y₁₂²⁺919.9

b₁₃²⁺939.8

y₁₃²⁺963.4

b₁₂-AMP

1384.5

y₁₂-AMP

1491.7

b₁₃-AMP1531.6

y₁₃-AMP1578.7

y₁₅-AMP1806.8

y₁₂1838.7

y₁₃1925.7

1045.5

1036.2

979.3

988.3

930.4

730.1

724.2

y₁₄3⁺680.3

m/z (full)

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MH₂²⁺-adenine





MH₃3⁺-3H₂O

MH₃3⁺-2H₂O


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f8_02_w01369

3934

At3; Serpinc1; AT3; mCG_9405; P32261; Q543J5

78.93

ITMS; CID; 1

_ S

y₈²⁺

b₂

L

y₇

b₃

N P N

b₆

adpr

R

y₃

V T F K _

a₂

173.1b₂

201.1


b₃-NH₃298.1

b₃315.2 AMP

348.1y₃

395.2

ADP428.0

b₆²⁺612.2

y₇²⁺701.8

y₈²⁺758.8

b₆-AMP876.4

y₇-AMP1055.5

782.5

773.2

685.4y₈3⁺

200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400

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MH₂²⁺-AMP

506.2


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f3_02_w01364

7524

Slc38a3; Sn1; Snat3; Q9DCP2

101.9

ITMS; CID; 1

ac

_ M E

y₁₄²⁺

b₃

I

y₁₃²⁺

P

y₁₂

b₅

adpr

R Q T

y₉

b₈²⁺

E

y₈²⁺

M

y₇

b₁₀

V

y₆

b₁₁²⁺

E

y₅

b₁₂

L

y₄

b₁₃

V P N G K _

AMP348.1

b₃-H₂O398.2

y₄415.2

b₃416.2

y₈²⁺428.3

y₅-NH₃497.3

y₅514.3

y₆627.4

y₁₂²⁺672.8

y₇756.4

b₈²⁺784.8

b₁₀²⁺899.8 b₁₁²⁺

964.4

y₉986.5

b₁₂²⁺1020.9

y₁₃²⁺1021.4

y₁₄²⁺1069.9

b₅-H₂O1192.4

b₅1210.4

y₁₂

1344.7

1451.6

1693.7

1792.8


MH₂²⁺-AMP

1201.0

1104.6

1095.3MH₂²⁺-AMP-H₂O

1153.1


b₁₀-AMP

b₁₂-AMP

b₁₃-AMP

400 600 800 1000 1200 1400 1600 1800 2000

m/z (full)

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4060

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0100

200300

400500

Intensity

400 600 800 1000 1200 1400 1600 1800 2000

m/z (zoom)


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f2_02_w01363

7446

Slc38a3; Sn1; Snat3; Q9DCP2

95.63

ITMS; CID; 1

ac

_ M

y₁₅²⁺

E

y₁₄²⁺

b₃

I

y₁₃²⁺

P

ri

R Q T

b₈

E

b₉²⁺

M

y₇

V

y₆

b₁₁²⁺

E

y₅

b₁₂²⁺

L

y₄

V P N

b₁₆²⁺

G K _

y₄-NH₃398.2

y₄415.2

b₃416.2

y₅-NH₃497.3

y₅514.3

b₈²⁺620.3

y₆627.4

b₉²⁺685.8 y₇

756.4

b₁₁²⁺799.8

b₁₂²⁺856.4

y₁₃²⁺856.9

y₁₄²⁺905.4

y₁₅²⁺962.0

b₁₆²⁺1040.0

b₈1239.5

y₁₄²⁺-NH₃897.2

300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600

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Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f3_01_w01048

4080

Snt2b1; Sntb1; Snt2b1; Sntb1; Q99L88-1; Q99L88; Q99L88-2; Q99L88

78.29

ITMS; CID; 1

ac

_ A

y₁₈²⁺

V

y₁₇²⁺

A

y₁₆²⁺

b₄

A

y₁₅²⁺

b₅

A

y₁₄²⁺

b₆

A

y₁₃²⁺

b₇

V

y₁₂²⁺

b₈

A

y₁₁²⁺

b₉

A P A G G G G

y₄

b₁₆²⁺

A

adpr

R A Q R _

AMP348.1

b₄355.2

b₅426.2

ADP428.0

b₆497.3

b₇596.3

b₁₆²⁺603.3

b₈667.4

b₉738.4

y₁₁²⁺769.8

y₁₂²⁺805.3

y₁₃²⁺840.8

y₁₄²⁺890.4

y₁₅²⁺925.9

y₁₆²⁺961.4

y₁₇²⁺996.9

y₁₈²⁺

1032.4

y₄

1071.4


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10001500

20002500

30003500

Intensity

300 400 500 600 700 800 900 1000 1100 1200 1300 1400

m/z (zoom)


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f4_01_w01049

2398

RP23-356J7.1-003; Snx12; mCG_2194; RP23-356J7.1-001; Snx12; Snx12; mCG_2194; RP23-356J7.1-002; Snx12; Q3V2H3; Q99M52; Q3TGS7; O70493; Q6ZWQ5

88.95

ITMS; CID; 1

ac

_

y₉²⁺

S

y₈²⁺

D

y₇²⁺

b₃

T

y₆²⁺

b₄

A

b₅

V A D T

adpr

R R _

b₃-H₂O328.1

b₃346.1

AMP348.1

b₄-H₂O399.2

b₄417.2

ADP428.0

b₅516.2

y₆²⁺629.7

y₇²⁺665.3

y₈²⁺715.8

y₉²⁺773.3

761.2

752.4



704.1


MH₂²⁺-AMP664.5

y₅²⁺

547.0

MH₂3⁺-2H₂O

509.9

y₉²⁺-H2Oy₉3⁺-H2O

764.1

020

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0

Rel

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unda

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0200

400600

8001000

12001400

Intensity

250 300 350 400 450 500 550 600 650 700 750 800 850

m/z (zoom)

580.2


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f8_02_w01369

10191

RP23-356J7.1-003; Snx12; mCG_2194; RP23-356J7.1-001; Snx12; Snx12; mCG_2194; RP23-356J7.1-002; Snx12; Q3V2H3; Q99M52; Q3TGS7; O70493; Q6ZWQ5

46.79

ITMS; CID; 1

_ L N S K P

b₆-NH₃

Q D L

y₂₆²⁺

T

b₁₀

D

y₂₄²⁺

b₁₁

A

y₂₃²⁺

b₁₂

Y

b₁₃

G

y₂₁²⁺

P P S

y₁₈²⁺

N F L

y₁₅

E

y₁₄²⁺

I

y₁₃

D

y₁₂²⁺

I

y₁₁²⁺

F

y₁₀²⁺

N

y₉

P Q T V G

b₃₁²⁺

V G

adpr

R A R _

y₂₂²⁺

ADP

428.0

b₆-NH₃651.3

b₁₂²⁺673.8

b₁₃²⁺702.3

y₉²⁺742.8

y₁₀²⁺791.3

y₁₁²⁺848.4

y₁₂²⁺921.9

y₁₃²⁺978.4

1009.5

y₁₄²⁺1035.9

b₁₀1112.6

b₁₁

1183.6

y₁₈²⁺1287.1

b₁₂1346.7

b₁₃1403.7

y₂₁²⁺1436.1

1484.6

y₂₃²⁺1513.2

y₂₄²⁺1594.7

b₃₁²⁺1686.3

y₂₆²⁺1687.7

y₁₅-AMP1836.9 y₁₃

1955.9

1374.7

1342.3

1278.0y₁₈²⁺-H₂O

1503.8

1585.4

y₂₃²⁺-H₂O

y₂₄²⁺-H₂O

990.2

y₂₂3⁺

y₂₂²⁺

y₂₃3⁺

1063.5

y₂₄3⁺

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MH₃3⁺-adenosine-H₂O

MH₃3⁺-AMP


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f4_02_w01365

8907

Snx2; Q9CWK8; Q3U5L3; Q91VZ1

61.52

ITMS; CID; 1

_ E L I

y₂₄²⁺

L

y₂₃²⁺

b₅

S

y₂₂²⁺

S

y₂₁²⁺

b₇

E P

y₁₉²⁺

b₉-H₂O

S P A

y₁₆²⁺

b₁₂

V

y₁₅²⁺

b₁₃

T

b₁₄

P

y₁₃²⁺

V

y₁₂²⁺

T P T T L

y₇²⁺

I A P

adpr

R I E S K _

ADP

428.0

b₅556.3

y₇²⁺671.3

b₇-H₂O754.4

b₇772.4

y₁₂²⁺933.9

b₉-H₂O938.5

y₁₃²⁺984.4

y₁₅²⁺1082.5

y₁₆²⁺1133.0

b₁₂-H₂O1205.6

b₁₂1223.7

y₁₉²⁺1266.6

b₁₃1324.7

y₂₁²⁺1358.7

b₁₄1421.8

y₂₂²⁺1423.2

y₂₃²⁺1466.7

y₂₄²⁺1510.2



1610.8


1570.6


MH₂²⁺-AMP

1561.8

1350.1

y₂₁²⁺-NH₃

400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700 1800 1900 2000

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Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f6_01_w01051

10728

mCG_121767; Snx3; Q78ZM0; Q9CSC2; Q9CU14

66.32

ITMS; CID; 1

_ A F L

adpr

R

b₅

Q L P

b₈²⁺

F

adpr

R G D

y₁₁²⁺

D G

y₉

b₁₄²⁺

I

y₈

b₁₅²⁺

F D

y₆

b₁₇²⁺

D

y₅

N

y₄

F

y₃

I

y₂

E E R _

y₂304.2

AMP348.1

ADP428.0

y₃433.2 y₄

546.3

y₉²⁺592.8

y₁₁²⁺

677.8173

y₅693.4 b₈²⁺

757.8

y₆807.4

y₈1037.5

b₅1157.4

y₉1184.5

b₁₄²⁺1335.0

y₁₁-NH₃1337.6

b₁₅²⁺1408.5

b₁₇²⁺1523.5

1240.0

MH₂3⁺-adenine

1234.0MH₂3⁺-adenine-H₂O

1201.7

MH₂3⁺-adenosine-H₂O

MH₂3⁺-AMP1169.2

890.6

b₁₄3⁺

939.3

b₁₅3⁺

1054.0

b₁₈3⁺

862.0

1103.4

b₁₉3⁺

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y₁₇3⁺


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f3_02_w01364

2075

mCG_121767; Snx3; Snx3; Q78ZM0; Q9CSC2; Q9CU14; O70492

96.25

ITMS; CID; 1

ac

_

y₈²⁺

A

y₇²⁺

b₂

E

y₆

b₃

T

b₄

V A D T

adpr

R R _

y₅²⁺

b₂243.1


b₃-H₂O326.1

b₃344.1

AMP348.1 b₄-H₂O

425.2

ADP428.0

b₄443.2

y₆²⁺629.7

y₇²⁺

680.3

y₈²⁺744.8

y₆-AMP911.4

733.9

724.9

715.7

627.7

619.0

580.3

y₅²⁺

200 300 400 500 600 700 800 900 1000 1100 1200

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20002500

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MH₂²⁺-adenine




MH₂²⁺-AMP


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f9_02_w01370

10105

mCG_121767; Snx3; Q78ZM0; Q9CSC2; Q9CU14

86.48

ITMS; CID; 1

_ A F L

adpr

R Q

y₁₇²⁺

b₆

L P F R G

y₁₂

D D G

y₉²⁺

b₁₄²⁺

I

y₈²⁺

b₁₅²⁺

F

y₇

b₁₆²⁺

D

y₆-NH₃

b₁₇²⁺

D

y₅

N

y₄

b₁₉²⁺

F

y₃

I

y₂

E E R _

y₂-NH₃287.1

y₂304.2

AMP

348.1

ADP428.0

y₃433.2

y₈²⁺519.2

y₄546.3

y₉²⁺592.8

b₆²⁺635.8

y₅693.4

y₆-NH₃790.4

y₇922.4

b₆-AMP923.4 y₁₇²⁺

1021.5

b₁₄²⁺1064.5

b₁₅²⁺1138.0

b₁₆²⁺1195.5

b₁₇²⁺1253.0

b₁₉²⁺

1383.6

y₁₂1469.7

988.4MH₂²⁺-AMP

710.1b₁₄3⁺

759.2

b₁₅3⁺

797.4

b₁₆3⁺

874.0

b₁₈3⁺960.6

b₂₀3⁺

1003.5b₂₁3⁺

y₉²⁺-NH₃693.4

891.0

b₁₄²⁺-AMP

400 600 800 1000 1200 1400 1600 1800

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800

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Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f3_01_w01048

6486

Kiaa0120; Tagln2; Q9WVA4

72.62

ITMS; CID; 1

_ G A S

y₁₃²⁺

Q

y₁₂²⁺

b₅

A

y₁₁²⁺

b₆

G

y₁₀²⁺

b₇

M

y₉²⁺

T G

y₇²⁺

b₁₀

Y

y₆²⁺

G

y₅

b₁₂

M

b₁₃-H₂O

P

adpr

R Q

b₁₆²⁺

I I _

AMP348.1

b₅-H₂O397.2

b₅415.2

b₆-H₂O454.2

b₆472.2

b₇-H₂O585.2

b₇603.3

y₆²⁺649.8

y₇²⁺678.3

y₉²⁺788.3

y₁₀²⁺838.8

y₁₁²⁺904.4

b₁₀924.4

y₁₂²⁺932.9

y₁₃²⁺968.4

b₁₆²⁺1074.4

b₁₂1112.4

y₅

1167.5

b₁₃-H₂O1191.5

1072.6

1063.5

966.4

1063.5

584.4

y₅²⁺

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MH₂²⁺-adenine



MH₂²⁺-AMP


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liverf7_02_w01368

8230

IPI00944697

70.49

ITMS; CID; 1

_ N

y₉²⁺

b₂

L

y₈

b₃

I

y₇²⁺

b₄-NH₃

F F C

b₇

Q M G

adpr

R R _

a₂200.1

b₂

228.1


b₃341.2 AMP

348.1

ADP428.0

b₄-NH₃

471.3

y₇²⁺748.3

y₈²⁺821.8

y₉²⁺

878.3

b₇923.4

y₈-AMP1295.5

915.4


818.23


585.8

y₉3⁺

754.2

y₈²⁺-adenine

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MH₂²⁺-AMP


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f7_01_w01052

3128

RP23-195K8.11-001; Tln1; mKIAA1027; Tln; Tln1; A2AIM8; Q0V930; Q3TBC3; Q3UHS6; Q80TM2; Q8C9Z8; P26039

77.06

ITMS; CID; 1

_ A

y₉²⁺

I

y₈²⁺

b₃

A

y₇²⁺

V

y₆

b₅

N

b₆-NH₃

P

adpr

R

y₃

b₈²⁺

D

y₂

P P R _


b₃256.2

y₂

272.1717

AMP348.1

y₃369.2

b₅469.3

b₆-NH₃549.3

y₆²⁺639.7

b₈²⁺689.8

y₇²⁺696.8

y₈²⁺746.3

y₉²⁺781.8

y₆-AMP931.4

806.5

797.6

788.7


700.5

1143.4

y₆-adenine

521.7y₉3⁺

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MH₂²⁺-adenine



MH₂²⁺-AMP


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liverf7_02_w01368

10102

Trp32; Txnl; Txnl1; Txnl1; Q8CDN6; Q3U8R9; Q3UBF4; Q3UNH3

53.26

ITMS; CID; 1

_ S M D F E E A E

y₂₃²⁺

b₉

adpr

R S

y₂₁²⁺

b₁₁

E P

y₁₉²⁺

T

y₁₈²⁺

Q

y₁₇²⁺

A

y₁₆²⁺

L

y₁₅²⁺

E

y₁₄²⁺

L

y₁₃

T

y₁₂²⁺

E D

b₂₂²⁺

D

b₂₃²⁺

I K E

y₆

D G

y₄

I

y₃

V P L R _

AMP348.1

y₃385.3

y₄484.3

y₆654.4

y₁₂²⁺685.4

y₁₄²⁺800.4

y₁₅²⁺

857.0

y₁₆²⁺921.5

b₁₁²⁺926.8

y₁₇²⁺978.0

y₁₈²⁺1013.5

y₁₉²⁺

1077.6

y₂₁²⁺1176.6

y₂₃²⁺1284.7

y₁₃1498.8

b₁₁-AMP1505.5

b₂₂²⁺1533.1

b₂₃²⁺1589.6

b₉1636.5

1357.1


1319.2

1286.4

1280.3

1274.7

785.1

y₂₁3⁺

918.0b₁₁²⁺-H₂O

300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700 1800 1900

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MH₂²⁺-AMP


MH₂²⁺-AMP-2H₂O


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f6_02_w01367

8877

Tuba1b; Tuba2; Tuba1c; Tuba6; Tuba4; Tuba4a; P05213; O89052; Q99KA2; P68368

78.32

ITMS; CID; 1

_

adpr

R

y₁₂²⁺

S I

y₁₀

Q

y₉

F

y₈

b₆²⁺

V

y₇

b₇

D

y₆

W

y₅

b₉

C P T G F K _

b₅²⁺b₄²⁺

AMP348.1

ADP428.0

y₇²⁺448.2

y₅549.3

y₁₀²⁺628.8

b₆²⁺636.7

b₇²⁺694.3

y₆709.3 y₁₂²⁺

749.4

b₉²⁺867.3

y₇895.4

y₈1010.4

y₉1109.5

b₆-H₂O1254.5

y₁₀1256.6

b₉-AMP1386.5

b₇

1387.5

1074.0

1064.7

MH₂²⁺-adenine




968.0MH₂²⁺-AMP

685.4b₇²⁺-H₂O

587.2b₅²⁺

578.5b₅²⁺-NH3

513.7

b₄²⁺

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200300

400500

600

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Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f6_01_w01051

8743

Tuba1c; Tuba6; Tuba8; P68373; Q3TIZ0; Q52L87; Q9JJZ2

79.47

ITMS; CID; 1

_

adpr

R T I

y₁₀

Q

b₅

F

y₈

b₆

V

y₇

b₇

D

y₆

W

y₅

b₉²⁺

C P

y₃

T

y₂-NH₃

G F K _

y₂-NH₃277.2

y₃-NH₃334.2

AMP348.1

y₃

351.2

ADP428.0

y₅549.3

643.8

b₇²⁺701.3

y₆709.3

b₉²⁺874.3

y₇895.4

y₈1010.4

b₅1187.4

y₁₀1256.6 b₆

1286.5 b₇1401.5

635.0b₆²⁺-H₂O

594.3

b₅²⁺

520.8b₄²⁺

512.4

b₄²⁺-NH3

b₆²⁺

717.5b₁₃3⁺

m/z (full)

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m/z (zoom)

1081.0

1071.7

MH₂²⁺-adenine

MH₂²⁺-adenine-2H₂O1062.9


966.0

974.8MH₂²⁺-AMP



Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f6_02_w01367

9685

Tubb2; Tubb2a; Tubb2b; mCG_1395; Q7TMM9; Q99J49; Q9CWF2; B2RSN3; Q8BK89

45.61

ITMS; CID; 1

_ G S Q Q Y

y₁₅

adpr

R

y₁₄

A

b₈

L

y₁₂

b₉²⁺

T

y₁₁

b₁₀

V P

b₁₂

E L T Q

y₅

Q

y₄

M

y₃

F D S K _

AMP348.1

y₃349.2

ADP

428.0

y₄496.2

y₅627.3

y₁₁²⁺662.3 y₁₂²⁺

711.9b₉²⁺773.8

b₁₀²⁺823.3

b₈-AMP

1098.5

b₁₀-AMP1298.6

y₁₁1323.625

y₁₂1422.7 b₁₂-AMP

1524.7

b₁₀-H₂O1627.6

y₁₄

1636.8

y₁₅

1707.9

1408.2

1417.3

1399.3

1360.6


1311.2

MH₂²⁺-AMP

1302.2


1510.5b₁₀-adenine

b₁₀-adenine-H₂O1492.4

814.1b₁₀²⁺-H₂O

805.2

b₁₀²⁺-2H₂O

865.14

y7-H₂O

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MH₂²⁺-adenine




Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f5_01_w01050

8893

Tubb4; Tubb3; Tubb2c; Tubb2c1; Q9D6F9; Q9ERD7; Q9CRT0; P68372; Q9CVR0; Q9DCR1

45.61

ITMS; CID; 1

_ G S Q Q

y₁₆²⁺

Y

adpr

R

y₁₄-NH₃

A

y₁₃

L

y₁₂²⁺

b₉²⁺

T

y₁₁

b₁₀

V P

b₁₂²⁺

E

y₈

L T Q Q

y₄

b₁₇²⁺

M

y₃

b₁₈²⁺

F D A K _

y₃333.2

AMP348.1

y₄480.2

y₁₁²⁺654.3

y₁₂²⁺703.9

b₉²⁺773.8

b₁₀²⁺823.3

b₁₂²⁺936.4

y₈968.5

y₁₆²⁺1195.0

b₁₇²⁺1237.0

y₁₁1307.6

b₁₈²⁺1310.6

y₁₃1507.7

y₁₄-NH₃1603.8

b₁₀1645.6

1409.2

MH₂²⁺-adenine

1400.0



MH₂²⁺-AMP

1294.4

814.4

b₁₀²⁺-H₂O

765.0b₉²⁺-H₂O

300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700 1800

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0100

200300

400500

600

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300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700 1800

m/z (zoom)


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f7_01_w01052

7393

Tubb4; Tubb3; Tubb2c; Tubb2c1; Tubb2; Tubb2a; Tubb5; Tubb2; Tubb2a; Tubb2b; mCG_1395; Q9D6F9; Q9ERD7; Q9CRT0; P68372; Q9CVR0; Q9DCR1; Q62363; P99024; Q80ZV2; Q7TMM9; Q99J49; Q9CWF2; B2RSN3; Q8BK89

78.94

ITMS; CID; 1

_

y₁₀²⁺

I

y₉²⁺

b₂

S

y₈²⁺

b₃

E

y₇²⁺

Q

y₆²⁺

F T A

y₃

b₈²⁺

M

y₂-NH₃

b₉²⁺

F

adpr

R R _

b₂201.1


b₃-H₂O312.2

b₃330.2

AMP348.1 ADP

428.0

b₈²⁺454.7

b₉²⁺528.2

y₆²⁺661.7

y₃-AMP672.3 y₇²⁺

735.3

y₈²⁺799.3

y₂-NH₃855.3

y₉²⁺863.8

y₁₀²⁺907.3

020

4060

8010

012

0

Rel

ativ

e Ab

unda

nce

0100

200300

400

Intensity

200 300 400 500 600 700 800 900 1000

m/z (zoom)

887.3

878.5

839.5

790.6

781.6




MH₂²⁺-AMP



Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f6_01_w01051

9745

Tubb5; P99024; Q80ZV2

61.1

ITMS; CID; 1

_ G S Q Q

y₁₆²⁺

Y

adpr

R A

y₁₃

L

y₁₂

b₉²⁺

T

y₁₁

b₁₀

V P

y₉

E L

b₁₄²⁺

T

y₆

Q

y₅

Q

y₄

b₁₇²⁺

V F D A K _

AMP348.1

ADP428.0

y₄480.2 y₅

579.3

y₁₁²⁺638.3

y₁₂²⁺687.9

y₆707.4

b₉²⁺773.8

b₁₀²⁺823.3

b₁₄²⁺1043.4 y₉

1049.6

y₁₆²⁺1179.0

b₁₇²⁺1221.0

y₁₁1275.7

y₁₂1374.7

y₁₃1475.8

b₉-H₂O1528.6

b₁₀1645.6


1384.0



1190.5

MH₃3⁺-2H₂O

MH₃3⁺-3H₂O

814.3

805.3

1169.6

629.5

300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700 1800 1900

m/z (full)

020

4060

8010

012

0

Rel

ativ

e Ab

unda

nce

050

100150

200250

Intensity

300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700 1800 1900

m/z (zoom)

1336.2

1286.9MH₂²⁺-AMP

MH₂²⁺-AMP-H₂O1277.9


962.1

956.3

b₁₀²⁺-H₂O

b₁₀²⁺-2H₂O

y₁₆²⁺-H2O

y₁₁²⁺-H2O


Source:

Scannumber:

Protein:

Peptide Score:

Method:

o05368

1778

Ubch10; Ube2c; mCG_17518; RP23-370H21.6-001; RP23-370H21.6-002; Ube2c; Q9D1C1; A2A4Z0; Q3UWQ1; A2A4Z1

87.43

ITMS; CID; 1

ac

_

y₁₅²⁺

A S Q

y₁₂²⁺

N

y₁₁²⁺

adpr

R

y₁₀

b₆

D P

y₈

A

y₇

A

y₆

b₁₀

A

y₅

S

y₄

V

y₃

A

y₂

A V R _

y₂274.2

y₃345.2

AMP348.1

y₄416.3

ADP428.0

y₁₀²⁺456.8

y₁₁²⁺514.3

y₅

515.3

y₆602.4

b₆²⁺628.2

y₇673.4

y₈744.4

b₁₀²⁺783.3

y₁₂²⁺862.9

y₁₀912.5

y₁₅²⁺1027.4

b₁₀-AMP1218.5

b₆1255.4

1016.3

1007.4

998.55

959.4


MH₂²⁺-adenosine-2H₂O950.2

910.5

685.2

711.0

705.3

MH₃3⁺-2H₂O

MH₃3⁺-3H₂O

y₁₅3⁺

020

4060

8010

012

0

Rel

ativ

e Ab

unda

nce

0200

400600

8001000

12001400

Intensity

1900

m/z (zoom)

MH₂²⁺-adenine



MH₂²⁺-AMP


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f6_02_w01367

5765

Ufm1; P61961

94.19

ITMS; CID; 1

_

y₁₁²⁺

I

y₁₀²⁺

a₂

T

y₉²⁺

L

y₈

b₄

T S

y₆

b₆

D P

adpr

R

y₃

L

y₂

P Y K _

a₂187.1

y₂

310.2AMP348.1

y₃-NH₃390.2

y₃407.2

ADP428.0

b₄429.3

b₆-H₂O613.3

b₆631.3 y₆²⁺

657.8

y₈²⁺758.8

y₉²⁺809.3

y₁₀²⁺865.9

y₁₁²⁺916.4

y₆-AMP967.5

y₈-AMP1169.5

y₆

1314.5

905.5

896.7

MH₂²⁺-adenine


799.6

MH₂²⁺-AMP

702.4

MH₂²⁺-ADPribose

200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600

m/z (full)

020

4060

8010

012

0

Rel

ativ

e Ab

unda

nce

01000

20003000

40005000

60007000

Intensity

200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600

m/z (zoom)


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f2_02_w01363

7873

Uroc1; Uroc1; Q8VC12; Q3UEL5

61.13

ITMS; CID; 1

ac

_ S S

y₁₆²⁺

b₃

L

y₁₅²⁺

Q

y₁₄²⁺

b₅

E

y₁₃²⁺

b₆

L C

y₁₁²⁺

b₈-NH₃

S

b₉-H₂O

G

y₉²⁺

L P

y₇²⁺

L

y₆

b₁₃²⁺

adpr

R

y₅

P

y₄

b₁₅²⁺

L P E N R _

b₃

330.2

AMP348.1

ADP428.0

y₄515.3

b₅587.3

y₅

628.3 b₆700.4

y₇²⁺711.8

y₆725.4

y₉²⁺816.9

y₁₁²⁺901.9

b₈-NH₃930.4

b₉-H₂O986.4

b₉-NH₃987.4

b₁₃²⁺1012.9

y₁₃²⁺1025.4

y₁₄²⁺1082.0

b₁₅²⁺1118.0

y₁₅²⁺1146.5

y₁₆²⁺1210.5

1307.7

MH₂²⁺-adenine


1250.6

1201.4

MH₂²⁺-AMP

1104.7MH₂²⁺-ADP ribose



1289.8

1298.4

400 600 800 1000 1200 1400 1600 1800 2000

m/z (full)

020

4060

8010

012

0

Rel

ativ

e Ab

unda

nce

020

4060

80

Intensity

300 400 500 600 700 800 900 1000 1100 1200 1300

m/z (zoom)


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liverf7_02_w01368

7142

BC025105; Vsig4; Q80WA3

96.01

ITMS; CID; 1

_ I

y₁₀

b₂

Q

y₉²⁺

b₃

C

y₈²⁺

b₄

I

b₅-NH₃

Y

y₆²⁺

b₆

D P L

y₃

b₉²⁺

adpr

R G Y R _

b₂-NH₃225.1

b₂242.1

AMP348.1

b₃-NH₃385.1

y₃395.2

b₃

402.2

ADP428.0 b₄

515.3

y₆²⁺651.8

b₅-NH₃661.3

y₈²⁺790.8

b₆793.4

y₉²⁺847.3

b₉²⁺850.8

y₁₀²⁺927.4

y₁₀-AMP1506.6

980.5

971.5


MH₂²⁺-AMP874.3

200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600

m/z (full)

020

4060

8010

012

0

Rel

ativ

e Ab

unda

nce

0500

10001500

2000

Intensity

200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600

m/z (zoom)

MH₂²⁺-adenine


Source:

Scannumber:

Protein:

Peptide Score:

Method:

liver_f6_02_w01367

7032

BC025105; Vsig4; Q80WA3

58.68

ITMS; CID; 1

_

y₁₁²⁺

I

y₁₀²⁺

b₂

Q

y₉²⁺

b₃

C

y₈²⁺

I

y₇²⁺

Y

y₆

b₆

D P L

y₃

ri

R G Y R _

b₂-NH₃225.1

b₂242.1

y₃395.2

b₃402.2

y₆²⁺487.2

y₇²⁺544.7

y₈²⁺626.3

y₉²⁺682.8

y₁₀²⁺762.8

b₆793.4

y₁₁²⁺826.9

y₆973.5

200 300 400 500 600 700 800 900 1000 1100 1200 1300

m/z (full)

020

4060

8010

012

0

Rel

ativ

e Ab

unda

nce

01000

20003000

40005000

Intensity

200 300 400 500 600 700 800 900 1000 1100 1200 1300

m/z (zoom)


supplementary methods - images.nature.com · supplementary methods ... all the raw data files...

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