supplementary material type i interferon receptors and

https://doi.org/10.1071/RD19430_AC © CSIRO 2020 Reproduction, Fertility and Development, 2020, 32(11), 953–966. Supplementary Material Type I interferon receptors and interferon-t-stimulated genes in peripheral blood mononuclear cells and polymorphonuclear leucocytes during early pregnancy in beef heifers G. D. Melo A , L. M. F. Pinto A , C. C. Rocha A , I. G. Motta A , L. A. Silva B , J. C. da Silveira B , A. M. Gonella-Diaza C , M. Binelli D and G. Pugliesi A,E A Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, Avenida Duque de Caxias Norte, 225, Campus Fernando Costa, University of Sa˜o Paulo, AQ1 13635-900, Pirassununga, SP, Brazil. B Department of Veterinary Medicine, School of Animal Science and Food Engineering, Avenida Duque de Caxias Norte, 225, Campus Fernando Costa, University of Sa˜o Paulo, 13635-900, Pirassununga, SP, Brazil. C North Florida Research and Education Center, Institute of Food and Agricultural Sciences, University of Florida, Marianna, 4932 Highway 162, Marianna, FL 32446, USA. D Department of Animal Sciences, University of Florida, 2250 Shealy Drive, Gainesville, FL 32608, USA. E Corresponding author. Email: [email protected] File S1. Agilent Bioanalyzer profiles of RNA extracted form PMNs and PBMCs. RIN: RNA Integrity Number. Table S1. Pearson’s coefficient correlation and P values between ISGs and IFNARs expression in PMNs and PBMCs on D16, D18, D20 and D0-D20 of pregnant and non-pregnant heifers.

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Page 1: Supplementary Material Type I interferon receptors and

https://doi.org/10.1071/RD19430_AC © CSIRO 2020 Reproduction, Fertility and Development, 2020, 32(11), 953–966. Supplementary Material

Type I interferon receptors and interferon-t-stimulated genes in peripheral blood mononuclear cells and polymorphonuclear leucocytes during early pregnancy in beef heifers

G. D. MeloA, L. M. F. PintoA, C. C. RochaA, I. G. MottaA, L. A. SilvaB, J. C. da SilveiraB, A. M.

Gonella-DiazaC, M. BinelliD and G. PugliesiA,E

ADepartment of Animal Reproduction, School of Veterinary Medicine and Animal Science, Avenida

Duque de Caxias Norte, 225, Campus Fernando Costa, University of Sa˜o Paulo, AQ1 13635-900,

Pirassununga, SP, Brazil.

BDepartment of Veterinary Medicine, School of Animal Science and Food Engineering, Avenida

Duque de Caxias Norte, 225, Campus Fernando Costa, University of Sa˜o Paulo, 13635-900,

Pirassununga, SP, Brazil.

CNorth Florida Research and Education Center, Institute of Food and Agricultural Sciences,

University of Florida, Marianna, 4932 Highway 162, Marianna, FL 32446, USA.

DDepartment of Animal Sciences, University of Florida, 2250 Shealy Drive, Gainesville, FL 32608,

USA.

ECorresponding author. Email: [email protected]

File S1. Agilent Bioanalyzer profiles of RNA extracted form PMNs and PBMCs. RIN: RNA

Integrity Number.

Table S1. Pearson’s coefficient correlation and P values between ISGs and IFNARs expression in

PMNs and PBMCs on D16, D18, D20 and D0-D20 of pregnant and non-pregnant heifers.

Page 2: Supplementary Material Type I interferon receptors and

File S1. Agilent Bioanalyzer profiles of RNA extracted form PMNs and PBMCs. RIN: RNA Integrity Number.

Page 3: Supplementary Material Type I interferon receptors and

Table S1. Pearson’s coefficient correlation and P values between ISGs and IFNARs expression in PMNs and PBMCs on D16, D18, D20 and D0-D20 of pregnant and non-

pregnant heifers

IFNAR I IFNAR II

D16 D18 D20 D0-D20 D16 D18 D20 D0-D20

PMNs ISG15 0.40 (NS) 0.27 (NS) 0.02 (NS) -0.05 (NS) 0.31 (NS) -0.13 (NS) -0.32 (NS) -0.04 (NS) OAS1 0.43 (NS) 0.11 (NS) 0.04 (NS) -0.004 (NS) -0.14 (NS) -0.23 (NS) -0.26 (NS) -0.10 (NS)

MX1 0.30 (NS) 0.37 (NS) 0.23 (NS) 0.36 (0.0005) 0.37 (NS) 0.20 (NS) 0.21 (NS) -0.14 (NS) MX2 0.47 (NS) -0.11 (NS) 0.26 (NS) -0.14 (NS) 0.66 (0.01) -0.16 (NS) -0.16 (NS) 0.27 (0.02)

PBMCs ISG15 0.08 (NS) 0.06 (NS) -0.34 (NS) -0.05 (NS) 0.01 (NS) -0.04 (NS) -0.44 (NS) -0.11 (NS) OAS1 0.02 (NS) 0.15 (NS) -0.34 (NS) -0.05 (NS) 0.06 (NS) 0.006 (NS) -0.66 (NS) -0.33 (NS)

MX1 0.09 (NS) 0.17 (NS) 0 (NS) 0.07 (NS) 0.06 (NS) -0.0001 (NS) -0.16 (NS) 0.20 (0.03) MX2 -0.25 (NS) -0.10 (NS) -0.35 (NS) -0.005 (NS) -0.15 (NS) -0.12 (NS) --0.36 (NS) -0.07 (NS)

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