supplementary material morphological and mitochondrial dna ... · email:...

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Invertebrate Systematics, 32, 159–195 © CSIRO 2018 doi:10.1071/IS17052_AC Supplementary material Morphological and mitochondrial DNA data reshuffle the taxonomy of the genera Atopochetus Attems, Litostrophus Chamberlin and Tonkinbolus Verhoeff (Diplopoda: Spirobolida: Pachybolidae), with descriptions of nine new species Piyatida Pimvichai A,F , Henrik Enghoff B , Somsak Panha C and Thierry Backeljau D,E A Department of Biology, Faculty of Science, Mahasarakham University, Mahasarakham 44150, Thailand. B Natural History Museum of Denmark, University of Copenhagen, Universitetsparken 15, DK-2100 Copenhagen Ø, Denmark. C Animal Systematics Research Unit, Department of Biology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand. D Royal Belgian Institute of Natural Sciences (RBINS), Vautierstraat 29, B-1000 Brussels, Belgium. E Evolutionary Ecology Group, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium. F Corresponding author. Email: [email protected]

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Page 1: Supplementary material Morphological and mitochondrial DNA ... · Email: piyatida_pimvichai@yahoo.com . Fig. S1. Phylogenetic relationships of Litostrophus and Atopochetus species

Invertebrate Systematics, 32, 159–195 © CSIRO 2018 doi:10.1071/IS17052_AC

Supplementary material

Morphological and mitochondrial DNA data reshuffle the taxonomy of the genera Atopochetus Attems, Litostrophus Chamberlin and Tonkinbolus Verhoeff (Diplopoda: Spirobolida: Pachybolidae), with descriptions of nine new species

Piyatida PimvichaiA,F, Henrik Enghoff B, Somsak PanhaC and Thierry BackeljauD,E ADepartment of Biology, Faculty of Science, Mahasarakham University, Mahasarakham 44150, Thailand. BNatural History Museum of Denmark, University of Copenhagen, Universitetsparken 15, DK-2100 Copenhagen Ø, Denmark. CAnimal Systematics Research Unit, Department of Biology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand. DRoyal Belgian Institute of Natural Sciences (RBINS), Vautierstraat 29, B-1000 Brussels, Belgium. EEvolutionary Ecology Group, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium. FCorresponding author. Email: [email protected]

Page 2: Supplementary material Morphological and mitochondrial DNA ... · Email: piyatida_pimvichai@yahoo.com . Fig. S1. Phylogenetic relationships of Litostrophus and Atopochetus species

Fig. S1. Phylogenetic relationships of Litostrophus and Atopochetus species based on maximum likelihood analysis (ML) and Bayesian Inference (BI) of 496 bp of 16S rRNA. Numbers at nodes indicate branch support based on bootstrapping (ML) / posterior probabilities (BI). Scale bar = 0.09 substitutions/site. # indicates branches which received < 50% ML bootstrap support, - indicates non-supported branches by posterior probability. Clade memberships and designations are shown as vertical bars.

Page 3: Supplementary material Morphological and mitochondrial DNA ... · Email: piyatida_pimvichai@yahoo.com . Fig. S1. Phylogenetic relationships of Litostrophus and Atopochetus species

Fig. S2. Phylogenetic relationships of Litostrophus and Atopochetus species based on maximum likelihood analysis (ML) and Bayesian Inference (BI) of 660 bp of COI. Numbers at nodes indicate branch support based on bootstrapping (ML) / posterior probabilities (BI). Scale bar = 0.08 substitutions/site. # indicates branches which received < 50% ML bootstrap support, - indicates non-supported branches by posterior probability. Clade memberships and designations are shown as vertical bars.

Page 4: Supplementary material Morphological and mitochondrial DNA ... · Email: piyatida_pimvichai@yahoo.com . Fig. S1. Phylogenetic relationships of Litostrophus and Atopochetus species

Fig. S3. Atopochetus cf. sumatranus Hof (specimen from Penchala, Malaysia) gonopods. A: anterior gonopod, anterior view. B: anterior gonopod, posterior view. C: left posterior gonopod. D: anterior gonopod, anterior view drawing. E: anterior gonopod, posterior view drawing. F: left posterior gonopod drawing.

Page 5: Supplementary material Morphological and mitochondrial DNA ... · Email: piyatida_pimvichai@yahoo.com . Fig. S1. Phylogenetic relationships of Litostrophus and Atopochetus species

Fig. S4. Atopochetus cf. sumatranus GBM (specimen from Gua Batu Madu), gonopods. A: anterior gonopod, anterior view. B: anterior gonopod, posterior view. C: left posterior gonopod. D: anterior gonopod, anterior view drawing. E: anterior gonopod, posterior view drawing. F: left posterior gonopod drawing. G: SEM, left posterior gonopod, posterior-mesal view. H: SEM, tip of posterior gonopod, mesal view. I: SEM, above = distalmost part of posterior gonopod, mesal view, below = mesal part of posterior gonopod, mesal view. J: SEM, female vulva (op = operculum).