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Supplementary Figure S1. 1 H- 15 N HSQC spectra of 15 N-labeled ZG16p titrated with Man-O-Thr (a), Man- O-peptide (b), Manα1-3Man (c) and Glc1-3Glc (d). Marked peaks are selected for the calculation of dissociation constants (Figure 4). 15 N 15 N 1 H 1 H Arg side chain (Hδ) G147 G33 S146 G147 G35 E29 S146 G33 S146 G147 G33 (a) Man-O-Thr (b) Mannose-O-peptide (c) Mana1-3Man (d) Glc1-3Glc 0 1 2 5 10 20 50 100 0 1 2 5 10 20 50 100 0 1 5 10 20 50 100 200 0 1 5 10 20 50 100 Molar ratio Ligand:prot ein Molar ratio Ligand:prot ein Molar ratio Ligand:prot ein Molar ratio Ligand:prot ein 1 H 1 H

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Supplementary Figure S1. 1 H- 15 N HSQC spectra of 15 N-labeled ZG16p titrated with Man- O - Thr (a), Man- O -peptide (b), Manα1-3Man (c) and Glc  1-3Glc (d). Marked peaks are selected for the calculation of dissociation constants (Figure 4). (a) Man- O -Thr. (b) Mannose- O -peptide. - PowerPoint PPT Presentation

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Page 1: Supplementary Figure S1

Supplementary Figure S1.1H-15N HSQC spectra of 15N-labeled ZG16p titrated with Man-O-Thr (a), Man-O-peptide (b), Manα1-3Man (c) and Glc1-3Glc (d). Marked peaks are selected for the calculation of dissociation constants (Figure 4).

15N

15N

1H 1H

Arg side chain (Hδ)

G147

G33

S146

G147

G35

E29

S146

G33

S146

G147

G33

(a) Man-O-Thr (b) Mannose-O-peptide

(c) Mana1-3Man (d) Glcb1-3Glc

0125

102050

100

0125

102050

100

015

102050

100200

0

1

5

10

20

50

100

Molar ratioLigand:protei

n

Molar ratioLigand:protei

n

Molar ratioLigand:protei

n

Molar ratioLigand:protei

n

1H 1H

Page 2: Supplementary Figure S1

(a) Man-O-Ser (b) Man-O-Thr

(c) Mana1-3Man (d) Glcb1-3Glc

Supplementary Figure S2. The observed electron density of each ligand. Fo-Fc electron density maps of each ligand are shown in gray mesh, countered at 1s level ((a), (c) and (d)) or 0.5 s level (b). (a) Man-O-Ser/ZG16p, (b) Man-O-Thr/ZG16p, (c) Manα1-3Man/ZG16p and (d) Glc1-3Glc/ZG16p.

Asp151

Tyr104 Tyr104

Asp151

Tyr104

Asp151

Asp151

Tyr104

Ser148

Ser148

Ser148Ser

148


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