supplementary figure legends

5
Supplementary Figure 1. (a) NECs and CECs isolated from all five patients were incubated on Matrigel for 16 h after irradiation with 4 Gy, and then photographed. (b) NECs and CECs isolated from all five patients were irradiated with 4 Gy. After incubation for 24 h, RT-PCR was used to assess the mRNA expression levels of MMP2, plasminogen and the 18S rRNA. The experiments were repeated in triplicate and the data shown represent typical results. (c) NECs and CECs isolated from all five patients were irradiated with 4 Gy. After incubation for 24 h, zymography was used to analyze the activities of MMP-2 and MMP-9. The experiments were repeated in triplicate, and the data shown represent typical results. (d) NECs and CECs isolated from all five patients were irradiated with 4 Gy. After incubation for 24 h, ELISA was used to analyze the generation of angiostatin. The results from three independent experiments are expressed as means ± SEM. Supplementary Figure 2. (a) NECs and CECs isolated from all five patients were irradiated with 4 Gy. After incubation for 24 h, ELISA was used to analyze the generation of IL-6. The results from three independent experiments are expressed as means ± SEM. Supplementary Figure 3. NECs and CECs isolated from patient A were incubated on Matrigel for 16 h after irradiation with various doses of IR (2, 3, 4, 6, 8 or 10 Gy), and then photographed.

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Page 1: SUPPLEMENTARY FIGURE LEGENDS

SUPPLEMENTARY FIGURE LEGENDS

Supplementary Figure 1. (a) NECs and CECs isolated from all five patients were incubated on Matrigel for 16 h after irradiation with 4 Gy, and

then photographed. (b) NECs and CECs isolated from all five patients were irradiated with 4 Gy. After incubation for 24 h, RT-PCR was used to

assess the mRNA expression levels of MMP2, plasminogen and the 18S rRNA. The experiments were repeated in triplicate and the data shown

represent typical results. (c) NECs and CECs isolated from all five patients were irradiated with 4 Gy. After incubation for 24 h, zymography was

used to analyze the activities of MMP-2 and MMP-9. The experiments were repeated in triplicate, and the data shown represent typical results. (d)

NECs and CECs isolated from all five patients were irradiated with 4 Gy. After incubation for 24 h, ELISA was used to analyze the generation of

angiostatin. The results from three independent experiments are expressed as means ± SEM.

Supplementary Figure 2. (a) NECs and CECs isolated from all five patients were irradiated with 4 Gy. After incubation for 24 h, ELISA was

used to analyze the generation of IL-6. The results from three independent experiments are expressed as means ± SEM.

Supplementary Figure 3. NECs and CECs isolated from patient A were incubated on Matrigel for 16 h after irradiation with various doses of IR

(2, 3, 4, 6, 8 or 10 Gy), and then photographed.

Page 2: SUPPLEMENTARY FIGURE LEGENDS

NECs

CECs

Cont 4 Gy

Patient A

Cont 4 Gy

Patient B

Cont 4 Gy

Patient C

Patient D Patient E

Cont 4 Gy Cont 4 Gy

Supplementary figure 1.

NECs

CECs

(A)

Page 3: SUPPLEMENTARY FIGURE LEGENDS

4 Gy

A B C D E

NECs

18S rRNA

MMP2

Patient

- + - + - + - + - +

18S rRNA

Plasminogen

CECs

A B C D EPatient

4 Gy - + - + - + - + - +

Supplementary figure 1.

Zymography

MMP2

4 Gy - + - + - + - + - +

NECs

A B C D EPatient

MMP9

(B)

(C) (D)

A B C D EPatient A B C D E

NECs CECs

Generation of angiostatin

Page 4: SUPPLEMENTARY FIGURE LEGENDS

Supplementary figure 2.

Generation of IL-6

A B C D EPatient A B C D E

NECs CECs

Page 5: SUPPLEMENTARY FIGURE LEGENDS

Supplementary figure 3.

NECs

Control 2 Gy 3 Gy 4 Gy 6 Gy 10 Gy

CECs

8 Gy