supplementary figure 1-8 - images.nature.com · vz 100μm wt disc1 li 0 100 200 300 400 number of...
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VZ
100μm
WTDisc1 LI
0
100
200
300
400
Num
ber o
f Brd
U-
posi
tive
cells
WT Disc1 LI
*
Brd
UB
rdU
/DA
PI
Supplementary Figure 1
SVZ
VZ
a
100μm
WTDisc1 LI
0
10
20
30
40
Num
ber o
f PH
3-po
sitiv
e ce
lls in
the
VZ
WT Disc1 LI
*
PH
3P
H3/
DA
PI
b
SVZ
VZ
VZ
100μm 0 10 20 30 40 50 60 (%)
1
2
3
4
5
6
7
8
9
10
Cel
l dis
tribu
tion
of b
in a
naly
sis
WTDisc1 LI
0
0.2
0.4
0.6
0.8
Mig
ratio
ndi
stan
ce
(A.U
.)
WT Disc1 LI**
*
**
Brd
UB
rdU
/DA
PI
Supplementary Figure 2
CP
IZ
VZ/SVZ
a
100μm 0
1
2
3
4
5
6
7
8
9
10
Cel
l dis
tribu
tion
of b
in a
naly
sis
WTDisc1 LI
0
0.2
0.4
0.6
0.8
Mig
ratio
ndi
stan
ce
(A.U
.)
WT Disc1 LI**
**
***
**
GFP
GFP
/DA
PI CP
IZ
VZ/SVZ
b
10 20 30 40 50 60 (%)
CP
IZ
VZ/SVZ
CP
IZ
VZ/SVZ
100μm 0
1
2
3
4
5
6
7
8
9
10
Cel
l dis
tribu
tion
of b
in a
naly
sis
Cont RNAiDISC1 RNAi
0
0.2
0.4
0.6
0.8
Mig
ratio
ndi
stan
ce
(A.U
.)
Cont RNAi DISC1 RNAi
WT **
***
**
*
GFP
GFP
/DA
PI
Supplementary Figure 3
CP
IZ
VZ/SVZ
a
100μm 0
1
2
3
4
5
6
7
8
9
10
Cel
l dis
tribu
tion
of b
in a
naly
sis
Cont RNAiDISC1 RNAi
0
0.2
0.4
0.6
0.8
Mig
ratio
ndi
stan
ce
(A.U
.)
Cont RNAi DISC1 RNAi
Disc1 LI
n.s.
GFP
GFP
/DA
PI CP
IZ
VZ/SVZ
b
10 20 30 40 50 60 (%)
10 20 30 40 50 60 (%)
CP
IZ
VZ/SVZ
CP
IZ
VZ/SVZ