Supplementary Figure 1

2-cluster 3-cluster

4-cluster 5-cluster

Red: stable co-clustering Blue: no co-clustering

Supplementary Figure 2

Supplementary Figure 3

Supplementary Figure 4

P=0.16

EGFR mutation

Supplementary Figure 5

KRAS mutation

EGFR mutation

Supplementary Figure 6

Supplementary Figure 7

147

10

5

17

1626

EGFR mutation

DUSP4 deletionp16 deletion

104

n=199

p=0.007

p=0.0001

p=0.00003

0

1E-16

2E-16

3E-16

4E-16

5E-16

6E-16

Wild typeEGFR

EGFRdel19

EGFRL858R

Supplementary Figure 8D

US

P4

mR

NA

con

c (u

M)

WT

- N

o E

GF

WT

- 3

0 m

in

WT

- 1

hr

WT

- 2

hrs

WT

- 4

hrs

WT

- 6

hrs

del1

9 -

No

EG

F

del1

9 -

30 m

in

del1

9 -

1 hr

del1

9 -

2 hr

s

del1

9 -

4 hr

s

del1

9 -

6 hr

s

L85

8R -

No

EG

F

L85

8R -

30

min

L85

8R -

1 h

r

L85

8R -

2 h

rs

L85

8R -

4 h

rs

L85

8R -

6 h

rs

H1650

GFP DUSP4-GFP

Supplementary Figure 9

GFP

DUSP4-GFP

27KD

70KD

ACTIN

H358

Vect

orD

USP

4A B

H1650

GFP DUSP4-GFP

H1650 cells24hrs afterelectroporation

H1650 cellsafter one week selection

Supplementary Figure 10