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Suppl. Figure 4 Amino acid Observed Mw(expt) Mw(calc) ppm Score Sequence position m/z Da Da 25 - 33 546.7761 1091.5376 1091.5169 19 35 -.DLIDEICSK.T 25 - 37 512.6048 1534.7927 1534.7549 25 21 -.DLIDEICSKTDVK.K 25 - 38 555.2926 1662.8559 1662.8498 4 21 -.DLIDEICSKTDVKK.V 38 - 45 536.7932 1071.5718 1071.5535 17 46 K.KVCYYALR.E 38 - 45 358.2041 1071.5903 1071.5535 34 33 K.KVCYYALR.E 38 - 49 523.9443 1568.8110 1568.7769 22 5 K.KVCYYALREDPR.S 39 - 45 472.7434 943.4722 943.4586 14 32 K.VCYYALR.E 39 - 49 481.2518 1440.7337 1440.6820 36 9 K.VCYYALREDPR.S 91 – 102 496.9257 1487.7554 1487.7191 24 28 K.SRYTLCLGNYNK.A 93 - 102 623.3071 1244.5996 1244.5859 11 61 R.YTLCLGNYNK.A 103 - 115 472.9448 1415.8124 1415.7694 30 43 K.ASDIVAKM PDLLK.S a 110 - 115 358.7112 715.4078 715.3938 19 14 K.MPDLLK.S 110 - 115 366.7135 731.4125 731.3887 32 29 K.M PDLLK.S a 116 – 131 563.2941 1686.8606 1686.8213 23 33 K.SKDYYGLSAN ASAAIR.E b 132 - 149 691.3409 2071.0009 2070.9568 21 22 R.EPSTCDNNFIEPPVEVPK.L 150 - 158 358.5428 1072.6066 1072.5625 41 31 K.LREASDNLR.G a underlined M was detected as the oxidized form b underlined N was detected as the deamidated form, after treatment with N-glycosydase A 25 DLIDEICSKT DVKKVCYYAL REDPR SKGAK PEGLMAIVID LSQKNATFAC 75 NLVSTLLKGT TDPKLKSRYT LCLGNYNKAS DIVAKMPDLL KSKDYYGLSA 125 NASAAIREPS TCDNNFIEPP VEVPKLREAS DNLR GLIDVI LVLVGFL

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Amino acid Observed Mw(expt) Mw(calc) ppm Score Sequence position m/z Da Da 25 - 33 546.7761 1091.5376 1091.5169 19 35 -.DLIDEICSK.T 25 - 37 512.6048 1534.7927 1534.7549 25 21 -.DLIDEICSKTDVK.K - PowerPoint PPT Presentation

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Page 1: Suppl. Figure 4

Suppl. Figure 4

Amino acid Observed Mw(expt) Mw(calc) ppm Score Sequence position m/z Da Da

25 - 33 546.7761 1091.5376 1091.5169 19 35 -.DLIDEICSK.T 25 - 37 512.6048 1534.7927 1534.7549 25 21 -.DLIDEICSKTDVK.K 25 - 38 555.2926 1662.8559 1662.8498 4 21 -.DLIDEICSKTDVKK.V 38 - 45 536.7932 1071.5718 1071.5535 17 46 K.KVCYYALR.E 38 - 45 358.2041 1071.5903 1071.5535 34 33 K.KVCYYALR.E 38 - 49 523.9443 1568.8110 1568.7769 22 5 K.KVCYYALREDPR.S 39 - 45 472.7434 943.4722 943.4586 14 32 K.VCYYALR.E 39 - 49 481.2518 1440.7337 1440.6820 36 9 K.VCYYALREDPR.S 91 – 102 496.9257 1487.7554 1487.7191 24 28 K.SRYTLCLGNYNK.A 93 - 102 623.3071 1244.5996 1244.5859 11 61 R.YTLCLGNYNK.A 103 - 115 472.9448 1415.8124 1415.7694 30 43 K.ASDIVAKMPDLLK.Sa

110 - 115 358.7112 715.4078 715.3938 19 14 K.MPDLLK.S 110 - 115 366.7135 731.4125 731.3887 32 29 K.MPDLLK.Sa

116 – 131 563.2941 1686.8606 1686.8213 23 33 K.SKDYYGLSANASAAIR.Eb 132 - 149 691.3409 2071.0009 2070.9568 21 22 R.EPSTCDNNFIEPPVEVPK.L 150 - 158 358.5428 1072.6066 1072.5625 41 31 K.LREASDNLR.G

aunderlined M was detected as the oxidized form bunderlined N was detected as the deamidated form, after treatment with N-glycosydase A

25 DLIDEICSKT DVKKVCYYAL REDPRSKGAK PEGLMAIVID LSQKNATFAC 75 NLVSTLLKGT TDPKLKSRYT LCLGNYNKAS DIVAKMPDLL KSKDYYGLSA 125 NASAAIREPS TCDNNFIEPP VEVPKLREAS DNLRGLIDVI LVLVGFL

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